CA2974398A1 - Methods for highly parallel and accurate measurement of nucleic acids - Google Patents
Methods for highly parallel and accurate measurement of nucleic acids Download PDFInfo
- Publication number
- CA2974398A1 CA2974398A1 CA2974398A CA2974398A CA2974398A1 CA 2974398 A1 CA2974398 A1 CA 2974398A1 CA 2974398 A CA2974398 A CA 2974398A CA 2974398 A CA2974398 A CA 2974398A CA 2974398 A1 CA2974398 A1 CA 2974398A1
- Authority
- CA
- Canada
- Prior art keywords
- mir
- primers
- pcr
- dna
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562116302P | 2015-02-13 | 2015-02-13 | |
| US62/116,302 | 2015-02-13 | ||
| US201562135923P | 2015-03-20 | 2015-03-20 | |
| US62/135,923 | 2015-03-20 | ||
| PCT/US2016/017920 WO2016131030A1 (en) | 2015-02-13 | 2016-02-14 | Methods for highly parallel and accurate measurement of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2974398A1 true CA2974398A1 (en) | 2016-08-18 |
Family
ID=56615681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2974398A Abandoned CA2974398A1 (en) | 2015-02-13 | 2016-02-14 | Methods for highly parallel and accurate measurement of nucleic acids |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20180010176A1 (https=) |
| EP (1) | EP3256607B1 (https=) |
| JP (1) | JP2018509178A (https=) |
| CN (1) | CN107636166A (https=) |
| CA (1) | CA2974398A1 (https=) |
| RU (1) | RU2017131622A (https=) |
| WO (1) | WO2016131030A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210214781A1 (en) * | 2016-02-14 | 2021-07-15 | Abhijit Ajit Patel | Measurement of nucleic acid |
| US11371087B2 (en) * | 2016-06-10 | 2022-06-28 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| EP3601593B1 (en) * | 2017-03-24 | 2021-12-22 | Bio-Rad Laboratories, Inc. | Universal hairpin primers |
| CN106835292B (zh) * | 2017-04-05 | 2019-04-09 | 北京泛生子基因科技有限公司 | 一步法快速构建扩增子文库的方法 |
| DE202019005627U1 (de) | 2018-04-02 | 2021-05-31 | Grail, Inc. | Methylierungsmarker und gezielte Methylierungssondenpanels |
| EP3833776A4 (en) * | 2018-08-06 | 2022-04-27 | Billiontoone, Inc. | DILUTION MARKER FOR QUANTIFICATION OF BIOLOGICAL TARGETS |
| WO2020069350A1 (en) | 2018-09-27 | 2020-04-02 | Grail, Inc. | Methylation markers and targeted methylation probe panel |
| BR112021018125A2 (pt) | 2019-10-25 | 2021-11-16 | Illumina Cambridge Ltd | Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado |
| US20230033987A1 (en) * | 2019-12-16 | 2023-02-02 | Cargill, Incorporated | Isolated oleosome composition and process for preparing it |
| US11211144B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Methods and systems for refining copy number variation in a liquid biopsy assay |
| US11475981B2 (en) | 2020-02-18 | 2022-10-18 | Tempus Labs, Inc. | Methods and systems for dynamic variant thresholding in a liquid biopsy assay |
| US11211147B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing |
| WO2021195277A1 (en) * | 2020-03-24 | 2021-09-30 | President And Fellows Of Harvard College | Multiplexed methods for detecting target rnas |
| JP2023524016A (ja) * | 2020-04-29 | 2023-06-08 | フリーノム ホールディングス，インク． | 結腸細胞増殖性障害を特定するためのｒｎａマーカと方法 |
| US10941453B1 (en) * | 2020-05-20 | 2021-03-09 | Paragon Genomics, Inc. | High throughput detection of pathogen RNA in clinical specimens |
| WO2021262422A1 (en) * | 2020-06-25 | 2021-12-30 | Huang Chung Ying | Method and system of dna and rna detection |
| CN112592968B (zh) * | 2020-12-27 | 2022-07-26 | 苏州科诺医学检验实验室有限公司 | 高通量测序用分子标签接头及其合成方法与应用 |
| CN115125295B (zh) * | 2022-03-10 | 2025-03-14 | 安徽师范大学 | 一种用于多位点可持续使用的基因分型标准品 |
| KR20250019610A (ko) | 2022-03-21 | 2025-02-10 | 빌리언투원, 인크. | 치료 모니터링을 위한 메틸화된 세포 유리 dna의 분자 계수 |
| US11680293B1 (en) | 2022-04-21 | 2023-06-20 | Paragon Genomics, Inc. | Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules |
| US12091715B2 (en) | 2022-04-21 | 2024-09-17 | Paragon Genomics, Inc. | Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6060240A (en) * | 1996-12-13 | 2000-05-09 | Arcaris, Inc. | Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom |
| US20040067492A1 (en) * | 2002-10-04 | 2004-04-08 | Allan Peng | Reverse transcription on microarrays |
| US7270983B1 (en) * | 2004-02-19 | 2007-09-18 | Research Foundation Of The University Of Central Florida, Inc. | Messenger RNA profiling: body fluid identification using multiplex reverse transcription-polymerase chain reaction (RT-PCR) |
| US20060019258A1 (en) * | 2004-07-20 | 2006-01-26 | Illumina, Inc. | Methods and compositions for detection of small interfering RNA and micro-RNA |
| JPWO2007141912A1 (ja) * | 2006-06-07 | 2009-10-15 | 住友ベークライト株式会社 | Ｒｎａ検出方法 |
| JP5299986B2 (ja) * | 2007-11-01 | 2013-09-25 | 国立大学法人山口大学 | 核酸の定量方法 |
| WO2012112804A1 (en) * | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| EP3395957B1 (en) * | 2011-04-25 | 2020-08-12 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| AU2012304328B2 (en) * | 2011-09-09 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| AU2013267609C1 (en) * | 2012-05-31 | 2019-01-03 | Board Of Regents, The University Of Texas System | Method for accurate sequencing of DNA |
| KR20200140929A (ko) * | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| WO2014152155A1 (en) * | 2013-03-14 | 2014-09-25 | The Broad Institute, Inc. | Massively multiplexed rna sequencing |
| MX2017010857A (es) * | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
-
2016
- 2016-02-14 EP EP16750045.3A patent/EP3256607B1/en active Active
- 2016-02-14 WO PCT/US2016/017920 patent/WO2016131030A1/en not_active Ceased
- 2016-02-14 JP JP2017560901A patent/JP2018509178A/ja active Pending
- 2016-02-14 CN CN201680021886.5A patent/CN107636166A/zh active Pending
- 2016-02-14 RU RU2017131622A patent/RU2017131622A/ru not_active Application Discontinuation
- 2016-02-14 US US15/544,834 patent/US20180010176A1/en not_active Abandoned
- 2016-02-14 CA CA2974398A patent/CA2974398A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| RU2017131622A3 (https=) | 2019-10-25 |
| EP3256607A1 (en) | 2017-12-20 |
| CN107636166A (zh) | 2018-01-26 |
| EP3256607A4 (en) | 2019-03-13 |
| RU2017131622A (ru) | 2019-03-13 |
| WO2016131030A4 (en) | 2016-10-20 |
| EP3256607B1 (en) | 2021-01-20 |
| WO2016131030A1 (en) | 2016-08-18 |
| US20180010176A1 (en) | 2018-01-11 |
| JP2018509178A (ja) | 2018-04-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20170814 |
|
| EEER | Examination request |
Effective date: 20210212 |
|
| FZDE | Discontinued |
Effective date: 20230718 |