JP2018509178A - 核酸の高度並列型および正確な測定方法 - Google Patents

核酸の高度並列型および正確な測定方法 Download PDF

Info

Publication number
JP2018509178A
JP2018509178A JP2017560901A JP2017560901A JP2018509178A JP 2018509178 A JP2018509178 A JP 2018509178A JP 2017560901 A JP2017560901 A JP 2017560901A JP 2017560901 A JP2017560901 A JP 2017560901A JP 2018509178 A JP2018509178 A JP 2018509178A
Authority
JP
Japan
Prior art keywords
primer
rna
pcr
dna
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017560901A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018509178A5 (https=
Inventor
アジット パテール，アビジット
アジット パテール，アビジット
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JP2018509178A publication Critical patent/JP2018509178A/ja
Publication of JP2018509178A5 publication Critical patent/JP2018509178A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2017560901A 2015-02-13 2016-02-14 核酸の高度並列型および正確な測定方法 Pending JP2018509178A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562116302P 2015-02-13 2015-02-13
US62/116,302 2015-02-13
US201562135923P 2015-03-20 2015-03-20
US62/135,923 2015-03-20
PCT/US2016/017920 WO2016131030A1 (en) 2015-02-13 2016-02-14 Methods for highly parallel and accurate measurement of nucleic acids

Publications (2)

Publication Number Publication Date
JP2018509178A true JP2018509178A (ja) 2018-04-05
JP2018509178A5 JP2018509178A5 (https=) 2018-10-25

Family

ID=56615681

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017560901A Pending JP2018509178A (ja) 2015-02-13 2016-02-14 核酸の高度並列型および正確な測定方法

Country Status (7)

Country Link
US (1) US20180010176A1 (https=)
EP (1) EP3256607B1 (https=)
JP (1) JP2018509178A (https=)
CN (1) CN107636166A (https=)
CA (1) CA2974398A1 (https=)
RU (1) RU2017131622A (https=)
WO (1) WO2016131030A1 (https=)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210214781A1 (en) * 2016-02-14 2021-07-15 Abhijit Ajit Patel Measurement of nucleic acid
US11371087B2 (en) * 2016-06-10 2022-06-28 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
EP3601593B1 (en) * 2017-03-24 2021-12-22 Bio-Rad Laboratories, Inc. Universal hairpin primers
CN106835292B (zh) * 2017-04-05 2019-04-09 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的方法
DE202019005627U1 (de) 2018-04-02 2021-05-31 Grail, Inc. Methylierungsmarker und gezielte Methylierungssondenpanels
EP3833776A4 (en) * 2018-08-06 2022-04-27 Billiontoone, Inc. DILUTION MARKER FOR QUANTIFICATION OF BIOLOGICAL TARGETS
WO2020069350A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
BR112021018125A2 (pt) 2019-10-25 2021-11-16 Illumina Cambridge Ltd Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado
US20230033987A1 (en) * 2019-12-16 2023-02-02 Cargill, Incorporated Isolated oleosome composition and process for preparing it
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
WO2021195277A1 (en) * 2020-03-24 2021-09-30 President And Fellows Of Harvard College Multiplexed methods for detecting target rnas
JP2023524016A (ja) * 2020-04-29 2023-06-08 フリーノム ホールディングス，インク． 結腸細胞増殖性障害を特定するためのｒｎａマーカと方法
US10941453B1 (en) * 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
WO2021262422A1 (en) * 2020-06-25 2021-12-30 Huang Chung Ying Method and system of dna and rna detection
CN112592968B (zh) * 2020-12-27 2022-07-26 苏州科诺医学检验实验室有限公司 高通量测序用分子标签接头及其合成方法与应用
CN115125295B (zh) * 2022-03-10 2025-03-14 安徽师范大学 一种用于多位点可持续使用的基因分型标准品
KR20250019610A (ko) 2022-03-21 2025-02-10 빌리언투원, 인크. 치료 모니터링을 위한 메틸화된 세포 유리 dna의 분자 계수
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
WO2012112804A1 (en) * 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
WO2013181170A1 (en) * 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
WO2014124338A1 (en) * 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US7270983B1 (en) * 2004-02-19 2007-09-18 Research Foundation Of The University Of Central Florida, Inc. Messenger RNA profiling: body fluid identification using multiplex reverse transcription-polymerase chain reaction (RT-PCR)
US20060019258A1 (en) * 2004-07-20 2006-01-26 Illumina, Inc. Methods and compositions for detection of small interfering RNA and micro-RNA
JPWO2007141912A1 (ja) * 2006-06-07 2009-10-15 住友ベークライト株式会社 Ｒｎａ検出方法
JP5299986B2 (ja) * 2007-11-01 2013-09-25 国立大学法人山口大学 核酸の定量方法
EP3395957B1 (en) * 2011-04-25 2020-08-12 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
AU2012304328B2 (en) * 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
WO2014152155A1 (en) * 2013-03-14 2014-09-25 The Broad Institute, Inc. Massively multiplexed rna sequencing
MX2017010857A (es) * 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
WO2012112804A1 (en) * 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
WO2013181170A1 (en) * 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
WO2014124338A1 (en) * 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation

Also Published As

Publication number Publication date
RU2017131622A3 (https=) 2019-10-25
EP3256607A1 (en) 2017-12-20
CN107636166A (zh) 2018-01-26
EP3256607A4 (en) 2019-03-13
RU2017131622A (ru) 2019-03-13
WO2016131030A4 (en) 2016-10-20
EP3256607B1 (en) 2021-01-20
WO2016131030A1 (en) 2016-08-18
US20180010176A1 (en) 2018-01-11
CA2974398A1 (en) 2016-08-18

Similar Documents

Publication Publication Date Title
JP2018509178A (ja) 核酸の高度並列型および正確な測定方法
US20240376533A1 (en) Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
CN112154216B (zh) 生物分子探针以及检测基因和蛋白表达的方法
EP2619329B1 (en) Direct capture, amplification and sequencing of target dna using immobilized primers
EP4247969A1 (en) Combined analysis of cell-free nucleic acids and single cells for oncology diagnostics
EP3539035B1 (en) Methods for expression profile classification
WO2018195217A1 (en) Compositions and methods for library construction and sequence analysis
AU2018429361A1 (en) Methods for the analysis of circulating microparticles
CN108699553A (zh) 用于筛选甲状腺癌中突变的组合物和方法
US20210214781A1 (en) Measurement of nucleic acid
CN104271770A (zh) 靶dna富集和测序
KR20220012980A (ko) 차세대서열결정법을 이용한 표적 단백질의 집단적 정량 방법과 그 용도
KR20170133270A (ko) 분자 바코딩을 이용한 초병렬 시퀀싱을 위한 라이브러리 제조방법 및 그의 용도
CN106591425A (zh) 基于连接反应的多重靶向检测核酸指标的方法
JP7759883B2 (ja) 並列化サンプル処理とライブラリー調製
WO2024073510A2 (en) Methods and compositions for fixed sample analysis
Deharvengt et al. Molecular assessment of human diseases in the clinical laboratory
US20250059530A1 (en) Labeling and analysis method for single-cell nucleic acid
Camps et al. New Technologies in Translational Medicine
CN107974486A (zh) 一种乳腺癌术后复发风险检测方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171011

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180726

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180911

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190115

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191029

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20191025

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200602