CN107624159B - 用于检查和处理显微的样本的方法和检查系统 - Google Patents

用于检查和处理显微的样本的方法和检查系统 Download PDF

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CN107624159B
CN107624159B CN201680029004.XA CN201680029004A CN107624159B CN 107624159 B CN107624159 B CN 107624159B CN 201680029004 A CN201680029004 A CN 201680029004A CN 107624159 B CN107624159 B CN 107624159B
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image
slide
laser microdissection
marking
information data
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CN107624159A (zh
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福尔克·施劳德拉夫
布拉戈维斯塔·韦格纳
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Leica Microsystems CMS GmbH
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Leica Microsystems CMS GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/32—Micromanipulators structurally combined with microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/70—Determining position or orientation of objects or cameras
    • G06T7/73—Determining position or orientation of objects or cameras using feature-based methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/282—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on with mapping; Identification of areas; Spatial correlated pattern
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving
    • G01N2001/2886—Laser cutting, e.g. tissue catapult
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30204—Marker

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Microscoopes, Condenser (AREA)
CN201680029004.XA 2015-05-20 2016-05-20 用于检查和处理显微的样本的方法和检查系统 Active CN107624159B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102015108017.6 2015-05-20
DE102015108017.6A DE102015108017A1 (de) 2015-05-20 2015-05-20 Verfahren und Untersuchungssystem zur Untersuchung und Bearbeitung einer mikroskopischen Probe
PCT/EP2016/061494 WO2016185040A1 (de) 2015-05-20 2016-05-20 Verfahren und untersuchungssystem zur untersuchung und bearbeitung einer mikroskopischen probe

Publications (2)

Publication Number Publication Date
CN107624159A CN107624159A (zh) 2018-01-23
CN107624159B true CN107624159B (zh) 2020-05-12

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CN201680029004.XA Active CN107624159B (zh) 2015-05-20 2016-05-20 用于检查和处理显微的样本的方法和检查系统

Country Status (6)

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US (1) US10533931B2 (enExample)
EP (1) EP3298380B1 (enExample)
JP (1) JP6743054B2 (enExample)
CN (1) CN107624159B (enExample)
DE (1) DE102015108017A1 (enExample)
WO (1) WO2016185040A1 (enExample)

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* Cited by examiner, † Cited by third party
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EP3250900B1 (en) * 2015-01-31 2021-08-18 Roche Diagnostics GmbH Systems and methods for meso-dissection
WO2016120433A1 (en) 2015-01-31 2016-08-04 Roche Diagnostics Gmbh Systems and methods for meso-dissection
CN110140040A (zh) 2016-11-09 2019-08-16 豪夫迈·罗氏有限公司 自动化的组织切割仪器及其使用方法
US11994664B2 (en) 2019-01-09 2024-05-28 Google Llc Augmented reality laser capture microdissection machine
DE102019102852B3 (de) * 2019-02-05 2020-07-02 Leica Microsystems Cms Gmbh Verfahren zur Lasermikrodissektion, Lasermikrodissektionssystem und Computerprogramm
DE102020100587B4 (de) * 2020-01-13 2025-12-24 Leica Microsystems Cms Gmbh Verfahren zum Überprüfen eines Dissektiervorgangs in einem Laser-Mikrodissektionssystem und Mittel zu dessen Durchführung
EP3904112A1 (en) * 2020-04-29 2021-11-03 Fa-Tech Diagnostics Italia S.R.L. Process for the inscription of glass microscope slides and glass microscope slides obtained thereby
JP2023525031A (ja) * 2020-05-08 2023-06-14 ダームテック，インク． 自動化されたサンプル走査および分離のシステムと方法
EP4246119B1 (en) 2022-03-17 2026-03-04 Leica Microsystems CMS GmbH Method and system for extracting a part of a microscopic sample
CN115979750B (zh) * 2022-12-14 2023-07-25 江苏医药职业学院 一种基于机器视觉的细胞载玻片精确点胶方法及系统
HUP2400294A1 (hu) * 2024-06-04 2025-12-28 Single Cell Tech Kft Eljárások biológiai minták gyors, automatizált, nagy áteresztõképességû vizsgálatára, valamint azok végrehajtására szolgáló rendszer
EP4737978A1 (en) * 2024-10-30 2026-05-06 Leica Microsystems CMS GmbH Controller for a laser microdissection system, laser microdissection system, and method for laser microdissection

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
DE10018253C2 (de) 2000-04-13 2003-08-21 Leica Microsystems Laser-Mikro-Dissektionsgerät
US20040085443A1 (en) * 2000-12-13 2004-05-06 Kallioniemi Olli P Method and system for processing regions of interest for objects comprising biological material
DE10152404C5 (de) * 2001-10-24 2017-06-08 Carl Zeiss Microscopy Gmbh Laser-Mikrodissektionssystem
CN101194263B (zh) * 2005-06-13 2012-02-22 三路影像公司 利用显微镜成像装置再定位载片上样品中的目标的系统和方法
DE102005028062C5 (de) * 2005-06-16 2015-10-22 Leica Microsystems Cms Gmbh Laser-Mikrodissektionsverfahren und Vorrichtung zur Laser-Mikrodissektion
US20080056952A1 (en) * 2006-08-25 2008-03-06 Angros Lee H Analytic plates with markable portions and methods of use
US8229194B2 (en) * 2006-11-16 2012-07-24 Visiopharm A/S Feature-based registration of sectional images
WO2009086521A2 (en) * 2007-12-28 2009-07-09 Carl Zeiss Microimaging Ais, Inc. System, device, and method for laser capture microdissection
AT505669B1 (de) * 2008-01-24 2009-03-15 Oridis Biomed Forschungs Und E Verfahren und vorrichtung zur manipulation mit proben
RU2553078C2 (ru) * 2009-04-28 2015-06-10 Конинклейке Филипс Электроникс Н.В. Способ микродиссекции и система обработки информации
US8744163B2 (en) * 2010-02-09 2014-06-03 International Genomics Consortium System and method for laser dissection
FI20115057L (fi) * 2011-01-21 2012-07-22 Wallac Oy Menetelmä ja laite yhden tai useamman näytealueen leikkaamiseksi näytteen kantajasta
JP6210882B2 (ja) * 2011-11-25 2017-10-11 オリンパス株式会社 組織分割装置、細胞分取装置、細胞分取システム、組織表示システム、組織分割方法および細胞分取方法
DE102012005587A1 (de) * 2012-03-20 2013-09-26 Metasystems Hard & Software Gmbh Automatische Kalibrierung eines Mikroskop-Scanningsystems
DE102012214664B4 (de) * 2012-08-17 2024-08-22 Leica Microsystems Cms Gmbh Objektträger mit Bezugspunkten
US20150262329A1 (en) * 2012-10-03 2015-09-17 KONINKLIJKE PHILIPS N.V. a corporation Combined sample examinations
DE102012218382B4 (de) * 2012-10-09 2015-04-23 Leica Microsystems Cms Gmbh Verfahren zum Festlegen eines Lasermikrodissektionsbereichs und zugehöriges Lasermikrodissektionssystem
DE102013216938B3 (de) * 2013-08-26 2015-01-08 Leica Microsystems Cms Gmbh Verfahren zur Kalibrierung einer Laserablenkeinrichtung einesLasermikrodissektionssystems und Lasermikrodissektionssystem
DE102013217532A1 (de) * 2013-09-03 2015-03-05 Siemens Aktiengesellschaft Mikrodissektionsgerät und Verfahren zum Isolieren von Zellen eines vorbestimmten Zelltyps

Also Published As

Publication number Publication date
EP3298380B1 (de) 2020-10-21
US10533931B2 (en) 2020-01-14
JP2018521309A (ja) 2018-08-02
JP6743054B2 (ja) 2020-08-19
DE102015108017A1 (de) 2016-11-24
EP3298380A1 (de) 2018-03-28
US20180149561A1 (en) 2018-05-31
WO2016185040A1 (de) 2016-11-24
CN107624159A (zh) 2018-01-23

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