CN107595706A - A kind of skin conditioning composition - Google Patents
A kind of skin conditioning composition Download PDFInfo
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Abstract
The present invention provides a kind of skin conditioning composition, it is characterised in that the skin conditioning composition includes：Butanediol, Bacillus acidi lactici/pear juice tunning filtrate, amur cork tree bark extract, white willow bark extract, Chinese glutinous rehmannia root extract, neem leaf extract, radix scutellariae root extract, houttuynia extract, Wild soybean albumen, Pi Aoning.
Description
Technical field
The present invention relates to a kind of plant extract skin care product, is more related to a kind of skin conditioning composition.
Background technology
Skin refers to tissue of the body surface bag outside muscle, is the maximum organ of human body.It is main carry protection body,
Perspire, feel the functions such as cold and hot and pressure.Skin covers whole body, and it makes various in vivo organize with organ from physical, mechanical
Property, the chemically invasion and attack with pathogenic microorganism.The skin of people and higher mammal is by epidermis, corium (mesoderm), hypodermis
Up of three layers.
According to the different stages of development and Morphological Features of cell, epidermis ecto-entad can be divided into 5 layers：Cuticula, hyaline layer,
Stratum granulosum, stratum spinosum epidermidis and basalis.
Cuticula is made up of several layers of keratinocyte, contains keratin.It can resist friction, prevent from blending chemicals outside body fluid
Invaded in matter.Keratin water absorbing force is stronger, and general water content is not less than 10%, to maintain the soft and moist of skin, is such as less than this value, skin
Then dry, the scales of skin that peel off or chap occur.Because position is different, its difference in thickness is very big, such as eyelid, foreskin, forehead, belly, the fossa cubitalis
Relatively thin Deng position, the palm, foot plantar position are most thick.The acellular core of cell of cuticula, if having, core is remaining, referred to as parakeratosis.Cuticula
It is the second layer of physical protection, its internal constituent possesses large effect to profound skin.
Sebaceous glands is mostly acinous gland, by the acinus of one or several cryptomeres and one between hair follicle and arrectores pilorum
Common tubulature is formed.Conduit is stratified squamous epithelium, is opened on hair follicle epimere mostly, and also some are directly open in skin table
Face.Acinus periphery is one layer of less juvenile cell, there is abundant organelle, and has active splitting ability, generates new gland
Cell.Sebum composition difference because of ethnic, hereditary, eating habit, the sebum of someone is saturated fatty acid, and fatty fusing point is high, point
Air then condenses furfur to the cold after secreting；The sebum of someone is unrighted acid, and fatty fusing point is low, and secretion thin oil makes skin light more
It is bright, hair bunchy.The secretion of sebum and the property of skin have substantial connection.
In view of the above-mentioned problems, the present invention provides a kind of skin conditioning composition, can prevent from activating sebaceous glands, stripping portion subangle
Matter layer, the effect of antibacterial is played, avoid the occurrence of the skin disease such as acne, acne.
The content of the invention
The present invention relates to a kind of skin conditioning composition, the skin conditioning composition includes：Butanediol, Bacillus acidi lactici/
Pear juice tunning filtrate, amur cork tree bark extract, white willow bark extract, Chinese glutinous rehmannia root extract, the extraction of azadirachta leaf
Thing, radix scutellariae root extract, houttuynia extract, Wild soybean albumen, Pi Aoning.
As one embodiment of the present invention, in parts by weight, the skin conditioning composition includes：
As one embodiment of the present invention, it is characterised in that in parts by weight, the skin conditioning composition bag
Contain：
As one embodiment of the present invention, it is characterised in that in parts by weight, the skin conditioning composition bag
Contain：
As one embodiment of the present invention, it is characterised in that the extracting method of the amur cork tree bark extract is as follows：
500g amur cork tree barks are taken, are ground to 100-200 mesh, 4000mL distilled water/alcohol mixeding liquid is added, is heated to reflux
12h, room temperature is cooled to, filtered, collect filtrate, add NaOH to pH=11, then added 500mL toluene, liquid separation, collect toluene
Layer, adds 15mL polyphosphoric acids, and 100mL distilled water is added after stirring, after standing 2 hours, liquid separation, removes toluene layer, subtracts
Pressure distillation, 100mL is concentrated into by liquor capacity, is filtered, and is collected solid, is added 800mL distilled water, be heated to reflux 45min, so
50mL saturation sodium sulfide solutions are added afterwards, are continued the 1h that flows back, are subsequently cooled to room temperature, add 100mL saturation calcium chloride solutions,
30min is stirred, filtering, collects filtrate, hydrochloric acid is added to pH=6, reuses Rotary Evaporators and solution is concentrated into 50mL, mistake
Filter, solid is collected, be dried in vacuo 12h, produce.
As one embodiment of the present invention, it is characterised in that the extracting method of the white willow bark extract is as follows：
500g white willow barks are taken, washing, after drying, 100-200 mesh is ground to, adds 1000mL distilled water and 5g Vitamin Cs
Acid, the method continuously extracted using ultrasonic adverse current, after being extracted 2 hours under conditions of 40-70 DEG C, centrifugation, filtrate is taken, it is rotated
Filtrate is evaporated by evaporimeter, obtains brown solid；Column chromatography for separation is carried out to brown solid, stationary phase uses neutral alumina,
First eluted with the first mobile phase, after elution completely, reuse the elution of the second mobile phase, collect the elution production of the second mobile phase
Thing, 4h is dried in vacuo, obtains dried object；Dried object is dissolved in 30mL 20wt% hydrochloric acid, after stirring 2 hours, adds 5mL
68wt% nitric acid, after 30min, after 5 DEG C are cooled in ice-water bath, collect solid, after deionized water is washed, dry,
Produce.
As one embodiment of the present invention, it is characterised in that the system of the Bacillus acidi lactici/pear juice tunning filtrate
Standby process is as follows：
(1) by European pear, clean, peeling, defibrination；1000g defibrinations are taken, add distilled water water, control the sugar content of defibrination
8~18%；
(2) dry ferment is added into composite fruit juice fermentation raw material, 28 DEG C~30 DEG C bars by 0.01%~0.05wt% additions
Ferment 9~17 days obtained one time fermentation liquid under part, and controlling pH is between 2g/L~5g/L；
(3) secondary fermentation：Saccharomycete, acetobacter aceti, lactobacillus acidophilus and lactobacillus species viable count >=0.8 ×
107Cfu/mL, by volume saccharomycete：Acetobacter aceti：Lactobacillus acidophilus：Bacillus acidi lactici=1：1：1：4 are made mixed bacteria；
Mixed bacteria is added into one time fermentation liquid by 8% inoculum concentration, ferment 1~3 day obtained composite fruit juice zymotic fluid under the conditions of 28 DEG C, hair
Zymotic fluid total acid is controlled in more than 4.5g/dL；
(4) above-mentioned zymotic fluid is taken, is filtered by vacuum, stood using diatomite, take filtrate.
As one embodiment of the present invention, it is characterised in that the extracting method of the neem leaf extract is as follows：
Azadirachta leaf 300g is taken, is cleaned, chopping, is added 2000mL absolute ethyl alcohols, 24h is soaked at 4 DEG C, is flowed back at 50 DEG C
Extraction 3 hours, filtering, filtrate is collected, is concentrated under reduced pressure into 200mL, added 200mL chloroforms, liquid separation, collect chloroform extract,
200mL 4wt%NaOH are added, liquid separation, collect water layer, hydrochloric acid is then added to pH=6, adds 200mL chloroforms, liquid separation, receive
Collect chloroform layer, with Rotary Evaporators solvent evaporated, obtain solid；
Solid is dispersed in water, adds 0.01g glucose and 0.2g/L spine root peroxidase and 5mL 20wt%
Hydrogen peroxide, stirring reaction is after 2 hours at 40 DEG C, filtering, after 10mL ether washs 3 times, dries, produces.
As one embodiment of the present invention, it is characterised in that the extracting method of the radix scutellariae root extract is as follows：
500g scutellariae,radixs are taken, are ground to 100-200 mesh, add 1000mL distilled water, the temperature of distilled water is 60-70
DEG C, after soaking 24 hours, filtering, taking precipitate；300mL benzene and 0.05g ferrocene is added in sediment, is heated to reflux 3
After hour, benzene liquid is collected, 100mL is concentrated into after cooling, add 100mL distilled water, collected distillation water layer, use Rotary Evaporators
Solvent evaporated, obtain solid, then using column chromatography, stationary phase is polyamide column, mobile phase be respectively 30vol% ethanol,
50vol% ethanol, 80vol% ethanol, first using 3000mL 30vol% ethanol elutions, then using 6000mL 50vol% second
Alcohol elutes, and finally using 4000mL 80vol% ethanol elutions, collects the eluent of 80vol% ethanol, is evaporated under reduced pressure to without second
Alcohol, 200mL absolute ethers are then added, filtering, filtrate are evaporated, obtain solid, 2h is dried in vacuo, produces.
The second aspect of the present invention provides a kind of skin conditioning agent, and it is prepared into by a kind of above-mentioned skin conditioning composition
Arrive, it is characterised in that the preparation process is as follows：
Each component is weighed by weight, the proud peace radix scutellariae root extract of skin is added in butanediol, after stirring, first
Sequentially add Bacillus acidi lactici/pear juice tunning filtrate, amur cork tree bark extract, it is to be mixed uniformly after, after being heated to 40 DEG C, according to
Secondary addition surplus materials, after stirring, is cooled to room temperature.
Embodiment
Participate in the election of the detailed description of the invention below for being preferable to carry out method and including embodiment this hair can be more easily understood
Bright content.Unless otherwise defined, all technologies used herein and scientific terminology have common with art of the present invention
The identical implication that technical staff is generally understood that.When contradiction be present, the definition in this specification is defined.
As used herein term " by ... prepare " it is synonymous with "comprising".Term "comprising" used herein, " comprising ",
" having ", " containing " or its any other deformation, it is intended that cover including for non-exclusionism.For example, the combination comprising listed elements
Thing, step, method, product or device are not necessarily limited to those key elements, but can include not expressly listed other key elements or
Such a composition, step, method, product or the intrinsic key element of device.
Conjunction " Consists of " excludes any key element do not pointed out, step or component.If be used in claim, this
Phrase will make claim be closed, it is not included the material in addition to the material of those descriptions, but relative normal
Except rule impurity.When being rather than immediately following in the clause that phrase " Consists of " appears in claim main body after theme,
It is only limited to the key element described in the clause；Other key elements are not excluded outside the claim as entirety.
Equivalent, concentration or other values or parameter are excellent with scope, preferred scope or a series of upper limit preferred values and lower limit
During the Range Representation that choosing value limits, this, which is appreciated that, specifically discloses by any range limit or preferred value and any scope
All scopes that any pairing of lower limit or preferred value is formed, regardless of whether the scope separately discloses.For example, when open
During scope " 1 to 5 ", described scope should be interpreted as including scope " 1 to 4 ", " 1 to 3 ", " 1 to 2 ", " 1 to 2 and 4 to
5 ", " 1 to 3 and 5 " etc..When number range is described herein, unless otherwise indicated, otherwise the scope is intended to include its end
Value and all integers and fraction within the range.
Singulative includes plural number and object is discussed, unless the context clearly dictates otherwise." optional " or it is " any
It is a kind of " refer to that the item that describes thereafter or event may or may not occur, and the description include situation that event occurs and
The situation that event does not occur.
Approximate term in specification and claims is used for modifying quantity, and it is specific to represent that the present invention is not limited to this
Quantity, include the part of the amendment of the acceptable change without cause related basic function close to the quantity.Phase
Answer, modify a numerical value with " about ", " about " etc., mean that the invention is not restricted to the exact numerical.It is approximate in some examples
Term likely corresponds to the precision of the instrument of measured value.In present specification and claims, scope limits can be with
Combine and/or exchange, these scopes include all subranges contained therebetween if not stated otherwise.
In addition, indefinite article " one kind " before key element of the present invention or component and "one" to key element or the quantitative requirement of component
(i.e. occurrence number) unrestriction.Therefore "one" or " one kind " should be read as including one or at least one, and odd number
The key element or component of form also include plural form, unless the obvious purport of the quantity refers to singulative.
The present invention provides a kind of skin conditioning composition, and the skin conditioning composition includes：Phellodendron extract, glutinous rehmannia carry
Thing, white willow bark extract, European pear fruit juice fermentation Product filtrate, neem leaf extract, Baical Skullcap root P.E, cordate houttuynia is taken to carry
Take thing, Pi Aoning, butanediol.
As one embodiment of the present invention, in parts by weight, the skin conditioning composition includes：
Bacillus acidi lactici/pear juice tunning filtrate
The pear juice, it is the juice of European pear, its tree is arbor, up to 15 meters, and tree crown is extensively conical；Sprig has thorn sometimes,
Blade is avette, subcircular to ellipse, umbrella shape raceme, 6-9, tool flower, bract film quality, wire lanceolar, and calyx tube is outer soft
Hair, sepal triangle lanceolar, petal obovate, white；Fruit obovate is subsphaeroidal, green, yellow, dilute band blush, has spot
Point, sepal harbor.April at florescence, the fruiting period 7-9 months.
According to record in Taiwan Department of Health nutrition ingredient in food data bank, 100 g of European pear, water content 83.5
G, 0.3 g of protein, fat 0.3 g, heat 60 block, 15.6 g of carbohydrate, also containing Alphalin, B2,
C, fiber, potassium, calcium, phosphorus, iron ... etc., meta-alkalescence food is belonged to, more fructose and glucose in contained sugared part, are the basic of salubrity
Nutrition, separately contain malic acid and citric acid, radiating diuresis of quenching one's thirst.Except internal gastrointestinal disturbances can be helped, it can also increase faecal volume, be
A kind of high fruit of nutritive value.
As one embodiment of the present invention, the preparation process of the Bacillus acidi lactici/pear juice tunning filtrate is as follows：
(1) by European pear, clean, peeling, defibrination；1000g defibrinations are taken, add distilled water water, control the sugar content of defibrination
8~18%；
(2) dry ferment is added into composite fruit juice fermentation raw material, 28 DEG C~30 DEG C bars by 0.01%~0.05wt% additions
Ferment 9~17 days obtained one time fermentation liquid under part, and controlling pH is between 2g/L~5g/L；
(3) secondary fermentation：Saccharomycete, acetobacter aceti, lactobacillus acidophilus and lactobacillus species viable count >=0.8 ×
107Cfu/mL, by volume saccharomycete：Acetobacter aceti：Lactobacillus acidophilus：Bacillus acidi lactici=1：1：1：4 are made mixed bacteria；
Mixed bacteria is added into one time fermentation liquid by 8% inoculum concentration, ferment 1~3 day obtained composite fruit juice zymotic fluid under the conditions of 28 DEG C, hair
Zymotic fluid total acid is controlled in more than 4.5g/dL；
(4) above-mentioned zymotic fluid is taken, is filtered by vacuum, stood using diatomite, take filtrate.
Amur cork tree bark extract
Golden cypress, alias：Amur cork tree, the bark that golden cypress is rutaceae amur cork tree or wampee, it is heat-clearing solution the effect of golden cypress
Poison, heat-clearing and damp-drying drug, the effect of golden cypress are to control damp-heat dysentery, diarrhea, jaundice, nocturnal emission, stranguria with turbid discharge, with inferior.
Main component：Containing small tiller alkali, golden cypress paulownia, obakulactone, dictamnolactone.
Pharmacological action：
1st, antibacterial：In vitro test is most strong to staphylococcus inhibitory action, to shigella dysenteriae, corynebacterium diphtheriae, pneumococcus, brain
Meningococcus and streptococcus also have stronger bactericidal action.
2nd, it is antimycotic：The similar coptis of effect, but effect is weaker.
3rd, anti-inflammatory is restrained：Zoopery confirms that golden cypress can mitigate contrafluxion.
In addition, also diuresis, stomach invigorating, external application promote the effects such as subcutaneous hemorrhage absorption
In the present invention, the extracting method of the amur cork tree bark extract is as follows：
500g amur cork tree barks are taken, are ground to 100-200 mesh, 4000mL distilled water/alcohol mixeding liquid is added, is heated to reflux
12h, room temperature is cooled to, filtered, collect filtrate, add NaOH to pH=11, then added 500mL toluene, liquid separation, collect toluene
Layer, adds 15mL polyphosphoric acids, and 100mL distilled water is added after stirring, after standing 2 hours, liquid separation, removes toluene layer, subtracts
Pressure distillation, 100mL is concentrated into by liquor capacity, is filtered, and is collected solid, is added 800mL distilled water, be heated to reflux 45min, so
50mL saturation sodium sulfide solutions are added afterwards, are continued the 1h that flows back, are subsequently cooled to room temperature, add 100mL saturation calcium chloride solutions,
30min is stirred, filtering, collects filtrate, hydrochloric acid is added to pH=6, reuses Rotary Evaporators and solution is concentrated into 50mL, mistake
Filter, solid is collected, be dried in vacuo 12h, produce.
In the distilled water/alcohol mixeding liquid, the volume ratio of distilled water and ethanol is 8：2.
White willow bark extract
White willow, Chinese medicine name.For the leaf, bud or root of Salicaceae sallow white willow.It is distributed in Xinjiang, Gansu, Qinghai, Tibet
Etc. ground.With clearing heat and detoxicating, the effect of dispelling wind and eliminating dampness.It is usually used in acute tonsillitis, sphagitis, the gland cheek is scorching, icterepatitis,
Pelvic infecton, ephritis, boil, rheumatic arthritis, rheumatoid arthritis.
In the present invention, the extracting method of the white willow bark extract is as follows：
500g white willow barks are taken, washing, after drying, 100-200 mesh is ground to, adds 1000mL distilled water and 5g Vitamin Cs
Acid, the method continuously extracted using ultrasonic adverse current, after being extracted 2 hours under conditions of 40-70 DEG C, centrifugation, filtrate is taken, it is rotated
Filtrate is evaporated by evaporimeter, obtains brown solid；Column chromatography for separation is carried out to brown solid, stationary phase uses neutral alumina,
First eluted with the first mobile phase, after elution completely, reuse the elution of the second mobile phase, collect the elution production of the second mobile phase
Thing, 4h is dried in vacuo, obtains dried object；Dried object is dissolved in 30mL 20wt% hydrochloric acid, after stirring 2 hours, adds 5mL
68wt% nitric acid, after 30min, after 5 DEG C are cooled in ice-water bath, collect solid, after deionized water is washed, dry,
Produce.
First mobile phase is by chloroform and methanol with volume ratio 6：1 is mixed to get, and the second mobile phase is by chloroform and methanol with body
Product ratio 1：1 is mixed to get.
Chinese glutinous rehmannia extract
Glutinous rehmannia, Scrophulariaceae glutinous rehmannia category herbaceos perennial.According to《Chinese book on Chinese herbal medicine》Effect is classified：Fresh rehmannia root is that heat-clearing is cool
Blood medicine；Prepared rhizome of rehmannia is then tonic.
The chemical composition of glutinous rehmannia is based on glucoside, wherein again based on iridoid glycosides.The cyclenes got from fresh rehmannia root
Ether terpene glucoside has：Motherwort glucoside, Aucubin, Catalpol, ground common glucoside A, B, C, D, the U.S. appropriate double glucosides of profit, all square rafter glucoside, square rafter
Glucoside, 8- table vomiting nuts glucoside acid, boat bone oxalic acid, 6-O-E- asafoetide acyl group ajugols, 6-O-Z- asafoetide acyl group ajugols, 6-O-
Vanilla acyl group ajugol, 6-O- is to fragrant acyl group ajugol, 6-O- (4 "-O- α-L- rhamnopyranosyloxyhies are good medicine glucoside) vanilla acyl group
Ajugol, burnt Rehmannioside A, B etc.；With the content highest of Catalpol.Contain carbohydrate again：D-Glucose, D- galactolipins, D-Fructose, sugarcane
Sugar, gossypose, stachyose, manninotriose, verbascose, with the content highest of stachyose, up to 64.9%.Also contain lysine, group
Propylhomoserin, arginine, aspartic acid, glutamic acid, threonine, edge propylhomoserin, glycine, alanine, valine, isoleucine, bright ammonia
The amino acid such as acid, tyrosine, phenylalanine, γ-aminobutyric acid and gucosamine, D- glucosides dew alcohol, phosphoric acid, gland glucoside and inorganic member
Element etc..
Root is containing Rehmannioside A, B, C, D, dihydro Catalpin, Aucubin, Catalpin.Fresh quench 0.11%. containing Catalpin is fresh
Still contain cupreol, mannitol, daucosterol, 1- ethyl-β-D- galactosides and sucrose in root alcohol extract.Water soluble ingredient
In contain a variety of sugar, be 32.1%~48.3% wherein with content of stachyose highest, and close several amino acids, wherein with arginine
Content highest, it is 2%~4.2%, separately has in Y aminobutyric acids, phosphoric acid commodity glutinous rehmannia and once got a small amount of cupreol, stigmasterol
And get series fatty acid, cupreol, palmitic acid, succinic acid, Daucosterol and S8 ring-types in micro campesterol dried rehamnnia roots
Compound.
In the present invention, the extracting method of the Chinese glutinous rehmannia extract is as follows：
40 parts of Chinese glutinous rehmannia are taken, four parts is equally divided into, washing, after drying, is ground to 100-200 mesh, is separately added into 50 parts
Methanol, it is heated to reflux, merges the extract solution of four times, methanol is then recovered under reduced pressure, obtained methanol extract is diluted with water, selects
Extracting n-butyl alcohol three times, takes n-butanol layer, and after being then concentrated under reduced pressure, add diethyl ether dissolving, takes brown color insoluble matter.To brown color not
Molten thing carries out column chromatography for separation, and stationary phase uses activated carbon diatomite, is eluted by the use of water as mobile phase, collects the elution of mobile phase
Product, 4h is dried in vacuo, dried object is obtained, produces.
Neem leaf extract
The leaf of chinaberry leaf, chinaberry leaf Meliaceae plant melia toosendan or China tree.Pharmacological action：The aqueous extract (2~5%) of China tree cotyledon can
The infringement to plant such as grasshopper, locust is prevented, and it is nontoxic to people, animal, and its active ingredient is carotenoids hydrocarbon.
Major function：Analgesic, desinsection.Control hernia, roundworm, treating swelling and pain by traumatic injury, furunculosis, skin eczema.①《Detailed outline》：Hernia enters capsule
Bitterly, beverage is decocted when facing hair.②《Guangdong Chinese medicine》Ⅱ：Bitter fruit leaf, grind external application.Treating swelling and pain by traumatic injury is controlled, only knife wound bleeding.
As one embodiment of the present invention, the extracting method of the neem leaf extract is as follows：
Azadirachta leaf 300g is taken, is cleaned, chopping, is added 2000mL absolute ethyl alcohols, 24h is soaked at 4 DEG C, is flowed back at 50 DEG C
Extraction 3 hours, filtering, filtrate is collected, is concentrated under reduced pressure into 200mL, added 200mL chloroforms, liquid separation, collect chloroform extract,
200mL 4wt%NaOH are added, liquid separation, collect water layer, hydrochloric acid is then added to pH=6, adds 200mL chloroforms, liquid separation, receive
Collect chloroform layer, with Rotary Evaporators solvent evaporated, obtain solid；
Solid is dispersed in water, adds 0.01g glucose and 0.2g/L spine root peroxidase and 5mL 20wt%
Hydrogen peroxide, stirring reaction is after 2 hours at 40 DEG C, filtering, after 10mL ether washs 3 times, dries, produces.
Azadirachta leaf possesses excellent performance, but the inventors discovered that the beneficial effect of azadirachta leaf can only be covered in skin
Skin top layer, it is impossible to be deep into skin nexine, can only cure the symptoms, not the disease, present invention combines its distinctive material feature, carries
For above-mentioned preparation method, the beneficial effect of azadirachta leaf can be increased, be deep into skin nexine, keep long-term activity.
Radix scutellariae root extract
Radix scutellariae, alias Radix Scutellariae, tsuchikane tea root, it is lamiaceae labiatae scutellaria herbaceos perennial；Meat rhizome is plump, leaf
Hard papery, lanceolar to wire lanceolar, raceme basidixed on stem and branch, corolla is purple, purplish red to blue, and filigree is flat,
Style is elongated, floral disc ring-type, ovary brown, pyrene ovoid.
The root of radix scutellariae is used as medicine, bitter, cold in nature, there is heat-clearing and damp-drying drug, purging intense heat and detonicating, hemostasis, antiabortive and other effects.Cure mainly warm
Disease, the infection of the upper respiratory tract, cough with lung heat, neonatal jaundice caused by dampness-heat, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swells furuncle
The diseases such as sore.The clinical antibiotic property of radix scutellariae is better than the coptis, and does not develop immunity to drugs.
Scutellariae,radix contains flavone compound：Baicalein, neobaicalein, i.e. skullcapflavone II, baicalin, wogonin, the Chinese
Baicalin, oroxylin A, 7- methoxyl group baicalein, skullcapflavone I, dihydro oroxylin A, Chrysin, 2,5,8- trihydroxies-
7- methoxy flavones, 2,5,8- trihydroxy -6,7- dimethoxy flavones, 4,5,7- trihydroxy -6- methoxy flavanones, 2,3,5,
6,7- penta hydroxy group flavanones, wogonin -5- β-D-Glucose glucoside, 2- (3- hydroxyl -4- methoxyphenyls)-ethyl -1-O- α -
L- rhamnopyranosyls (1 → 3)-β-D (4- asafoetides acyl group)-glucose, Chrysin -6-C- α-L-arabinose glucoside, Chrysin -6-C-
α-L-arabinose glucoside -8-C- β-D-Glucose glucoside, (2S) -2,5,6,7- tetrahydroxy flavanones, 5,7,2,6- kaempferols,
5,8- dihydroxy -6,7- dimethoxy flavones, 5,7,4- trihydroxy -8- methoxy flavones, oroxylin A-7-O- grape alditols
Sour glucoside, 5,7,2- trihydroxy methoxy flavones, 5,2- dihydroxy -6,7,8- trimethoxy flavones, 5- gate of a lane base -7,8- dimethoxys
Flavones, Norwogonin, dihydro baicalein, 5,7,2- trihydroxyflavones, 5,7,2- trihydroxy -8,6- dimethoxy flavones,
5,7,2,5- tetrahydroxy -8,6- dimethoxy flavones are viscidulin Ⅲ, 5,2,5- trihydroxy trimethoxy flavones, radix scutellariae
Element -7-O- β-D- glucopyanosides, 5,7,2- trihydroxy -8- methoxy flavones, 5,7,2,3- kaempferols, 5,7,2,3-
Four great base flavones, 3,5,7,2,5,6- pentahydroxyflavones are viscidulin Ⅰ, (2S) -7,2,6- trihydroxy -5- methoxyl group flavane
Ketone, 2,6,2,4- tetrahydroxy -6- methoxyl group chalcones, 5,7,2,5- kaempferols, left-handed eriodictyol, Sculellaria barbata kind are plain and viscous
Hair -2-O- β-D- glucopyanosides of baicalein III etc..Also contain cupreol, campesterol and stigmasterol in addition.
As one embodiment of the present invention, the extracting method of the radix scutellariae root extract is as follows：
500g scutellariae,radixs are taken, are ground to 100-200 mesh, add 1000mL distilled water, the temperature of distilled water is 60-70
DEG C, after soaking 24 hours, filtering, taking precipitate；300mL benzene and 0.05g ferrocene is added in sediment, is heated to reflux 3
After hour, benzene liquid is collected, 100mL is concentrated into after cooling, add 100mL distilled water, collected distillation water layer, use Rotary Evaporators
Solvent evaporated, obtain solid, then using column chromatography, stationary phase is polyamide column, mobile phase be respectively 30vol% ethanol,
50vol% ethanol, 80vol% ethanol, first using 3000mL 30vol% ethanol elutions, then using 6000mL 50vol% second
Alcohol elutes, and finally using 4000mL 80vol% ethanol elutions, collects the eluent of 80vol% ethanol, is evaporated under reduced pressure to without second
Alcohol, 200mL absolute ethers are then added, filtering, filtrate are evaporated, obtain solid, 2h is dried in vacuo, produces.
Contain enzyme in scutellariae,radix, certain humidity and at a temperature of, can digest two kinds of glucosides in radix scutellariae, i.e., baicalin and
Wogonoside, produce glucuronic acid and two kinds of glucoside members, i.e. baicalein and wogonin.And baicalein therein, it is a kind of adjacent
The trihydroxyflavone of position, itself is unstable, using this extracting method of the present invention it is possible to prevente effectively from the generation of this case,
The effective performance of product is not interfered with, while in the preparation process in later stage, can by the proud peaceful mixing in advance of Baical Skullcap root P.E and skin
To avoid the unstable of the two materials.
Houttuynia extract
Cordate houttuynia is the herbal medicine that Chinese Pharmacopoeia is included, and herbal medicine source is the dry aerial parts of saururaceae plant houttuynia cordata.
Cordate houttuynia acrid flavour, cold, return lung warp.Can clearing heat and detoxicating, detumescence treat sore, promoting urination and removing dampness, clearing away heat to cure dysentery, stomach strengthening and digestion promoting, with controlling
Real heat, heat toxin, damp evil, disease heat are the lung carbuncle of trouble, sore swollen toxin, hemorrhoidal hemorrhage, retention of heat in the spleen and stomach etc..Modern pharmacology, which is tested, to be shown,
This product has the effect such as antibacterial, antiviral, raising immunity of organisms, diuresis.
Cordate houttuynia aerial part contains volatile oil, includes antibiotic effective ingredient decanoyl acetaldehyde, lauryl aldehyde, a- firpenes (a-
Pinene) and linalool, the above two simultaneously have special foul smell.Also n-nonyl ketone containing methyl, lauro lene, laurene, limonene, acetic acid
Borneol acetate, cloves alkene.Usually said decanoy acetaldehyde refers to the addition product of the bisulfite pin of capric acid acetaldehyde.Separately contain A Fu
Glucoside, hyperin, rutin, chlorogenic acid and cupreol, stearic acid, oleic acid, linoleic acid.Leaf contains quercitrin, flower and fruit ear
Containing Isoquercitrin.
In the present invention, the extracting method of the houttuynia extract is as follows：Fresh cordate houttuynia is taken, it is rapid to clean, segment, add
The water of 10 times of weight of cordate houttuynia piece, and the Ginger Polysaccharide of 0.05 times of weight of cordate houttuynia, 1h is soaked, decocted 2 hours, filtering, the dregs of a decoction
The water of 8 times of weight of medicinal material is added, is decocted 1 hour, decoction filters out, and merges decoction liquor twice, is concentrated into 2 times of weight of cordate houttuynia
Boil liquid, ethanol is added the percent by volume of alcohol content is stirred, is stood, filtering is stand-by up to 75%.
Wild soybean albumen
Wild soybean, pulse family Glycine annual herb plant.Alias：Wu Dou, wild soya bean etc..First focused protection of country
Wild plant, second class protection plant.China's most area is distributed.Complete stool is the feed of domestic animal eating, can plant and make herbage, green
Fertilizer and soil-and-water conservation effect.Seed and root, stem, leaf can be used as medicine.
Wild soybean herb tonifying speen and tonifying kidney controls spontaneous perspiration, and human metabolism is of great benefit to, night sweat wandering arthritis hidrosis.Seed is sweet
Warm-natured, vinegar soya bean will fry soya bean and be put into porcelain vase, and there is the effect of flat liver improving eyesight, strong to control dizziness, blurred vision lumbago due to the kidney deficiency,
Therefore its institute's heat content is become for the food with effect of weight reducing, arthralgia and myalgia, often feeding less than this feature of other foods
Vinegar soya bean not only can reduce the delicate smooth wrinkle of skin, children's indigestion.
In the present invention, the Wild soybean albumen purchase is liked from the strong peace in the U.S..
Skin is proud peaceful
Tasteless yellow crystalline powder, it is the disinfecting that a kind of safety can be long-term use of to be dissolved in 1,3-BDO and propane diols
Agent, antibacterial action, the particularly propionic acid to causing comedo and acne can be produced to bacterium and a variety of fungies under relatively low concentration
Bacillus has stronger antibacterial action, can preferably improve acne and multiple acne symptom.
The proud peaceful purchase of heretofore described skin is former from Japanese woods.
The second aspect of the present invention provides a kind of skin conditioning agent, and it is prepared into by a kind of above-mentioned skin conditioning composition
Arrive, it is characterised in that the preparation process is as follows：
Each component is weighed by weight, the proud peace radix scutellariae root extract of skin is added in butanediol, after stirring, first
Sequentially add Bacillus acidi lactici/pear juice tunning filtrate, amur cork tree bark extract, it is to be mixed uniformly after, after being heated to 40 DEG C, according to
Secondary addition surplus materials, after stirring, is cooled to room temperature.
In the preparation method of the present invention, skin it is proud it is peaceful be photoactive substance, need to be placed in colored bottle during conventional storage,
Although the skin conditioning composition being added in the present invention is fewer, it is also susceptible to the influence of light source, treats one
For the people of a little sensitiveness skin, skin is proud peaceful with potential dangerous.In the preparation process of the present invention, first by the proud peace radix scutellariae of skin
Extract stirs together, adds surplus materials, it is possible to prevente effectively from Pi Aoning this shortcoming, improves the stabilization of product, no
It can cause allergic reaction so that this product can be directed to various people and use.
As one embodiment of the present invention, in parts by weight, the skin conditioning composition includes：
As one embodiment of the present invention, in parts by weight, the skin conditioning composition includes：
The present embodiment of embodiment 1. provides a kind of skin conditioning composition, and the skin conditioning composition includes：It is yellow
Cypress extract, glutinous rehmannia extract, white willow bark extract, European pear fruit juice fermentation Product filtrate, neem leaf extract, radix scutellariae
Extract, houttuynia extract, Pi Aoning, butanediol.
A kind of skin conditioning composition described in the embodiment 1 of embodiment 2., in parts by weight, the skin condition
Composition includes：
A kind of skin conditioning composition described in the embodiment 1 of embodiment 3., in parts by weight, the skin condition
Composition includes：
A kind of skin conditioning composition described in the embodiment 1 of embodiment 4., in parts by weight, the skin condition
Composition includes：
A kind of skin conditioning composition described in the embodiment 1 of embodiment 5., the extraction of the amur cork tree bark extract
Method is as follows：
500g amur cork tree barks are taken, are ground to 100-200 mesh, 4000mL distilled water/alcohol mixeding liquid is added, is heated to reflux
12h, room temperature is cooled to, filtered, collect filtrate, add NaOH to pH=11, then added 500mL toluene, liquid separation, collect toluene
Layer, adds 15mL polyphosphoric acids, and 100mL distilled water is added after stirring, after standing 2 hours, liquid separation, removes toluene layer, subtracts
Pressure distillation, 100mL is concentrated into by liquor capacity, is filtered, and is collected solid, is added 800mL distilled water, be heated to reflux 45min, so
50mL saturation sodium sulfide solutions are added afterwards, are continued the 1h that flows back, are subsequently cooled to room temperature, add 100mL saturation calcium chloride solutions,
30min is stirred, filtering, collects filtrate, hydrochloric acid is added to pH=6, reuses Rotary Evaporators and solution is concentrated into 50mL, mistake
Filter, solid is collected, be dried in vacuo 12h, produce.
A kind of skin conditioning composition described in the embodiment 1 of embodiment 6., the extraction of the white willow bark extract
Method is as follows：
500g white willow barks are taken, washing, after drying, 100-200 mesh is ground to, adds 1000mL distilled water and 5g Vitamin Cs
Acid, the method continuously extracted using ultrasonic adverse current, after being extracted 2 hours under conditions of 40-70 DEG C, centrifugation, filtrate is taken, it is rotated
Filtrate is evaporated by evaporimeter, obtains brown solid；Column chromatography for separation is carried out to brown solid, stationary phase uses neutral alumina,
First eluted with the first mobile phase, after elution completely, reuse the elution of the second mobile phase, collect the elution production of the second mobile phase
Thing, 4h is dried in vacuo, obtains dried object；Dried object is dissolved in 30mL 20wt% hydrochloric acid, after stirring 2 hours, adds 5mL
68wt% nitric acid, after 30min, after 5 DEG C are cooled in ice-water bath, collect solid, after deionized water is washed, dry,
Produce.
A kind of skin conditioning composition described in the embodiment 1 of embodiment 7., the Bacillus acidi lactici/pear juice tunning
The preparation process of filtrate is as follows：
(1) by European pear, clean, peeling, defibrination；1000g defibrinations are taken, add distilled water water, control the sugar content of defibrination
8~18%；
(2) dry ferment is added into composite fruit juice fermentation raw material, 28 DEG C~30 DEG C bars by 0.01%~0.05wt% additions
Ferment 9~17 days obtained one time fermentation liquid under part, and controlling pH is between 2g/L~5g/L；
(3) secondary fermentation：Saccharomycete, acetobacter aceti, lactobacillus acidophilus and lactobacillus species viable count >=0.8 ×
107Cfu/mL, by volume saccharomycete：Acetobacter aceti：Lactobacillus acidophilus：Bacillus acidi lactici=1：1：1：4 are made mixed bacteria；
Mixed bacteria is added into one time fermentation liquid by 8% inoculum concentration, ferment 1~3 day obtained composite fruit juice zymotic fluid under the conditions of 28 DEG C, hair
Zymotic fluid total acid is controlled in more than 4.5g/dL；
(4) above-mentioned zymotic fluid is taken, is filtered by vacuum, stood using diatomite, take filtrate.
A kind of skin conditioning composition described in the embodiment 1 of embodiment 8., the extraction of the neem leaf extract
Method is as follows：
Azadirachta leaf 300g is taken, is cleaned, chopping, is added 2000mL absolute ethyl alcohols, 24h is soaked at 4 DEG C, is flowed back at 50 DEG C
Extraction 3 hours, filtering, filtrate is collected, is concentrated under reduced pressure into 200mL, added 200mL chloroforms, liquid separation, collect chloroform extract,
200mL 4wt%NaOH are added, liquid separation, collect water layer, hydrochloric acid is then added to pH=6, adds 200mL chloroforms, liquid separation, receive
Collect chloroform layer, with Rotary Evaporators solvent evaporated, obtain solid；
Solid is dispersed in water, adds 0.01g glucose and 0.2g/L spine root peroxidase and 5mL 20wt%
Hydrogen peroxide, stirring reaction is after 2 hours at 40 DEG C, filtering, after 10mL ether washs 3 times, dries, produces.
A kind of skin conditioning composition described in the embodiment 1 of embodiment 9., the extraction side of the radix scutellariae root extract
Method is as follows：
500g scutellariae,radixs are taken, are ground to 100-200 mesh, add 1000mL distilled water, the temperature of distilled water is 60-70
DEG C, after soaking 24 hours, filtering, taking precipitate；300mL benzene and 0.05g ferrocene is added in sediment, is heated to reflux 3
After hour, benzene liquid is collected, 100mL is concentrated into after cooling, add 100mL distilled water, collected distillation water layer, use Rotary Evaporators
Solvent evaporated, obtain solid, then using column chromatography, stationary phase is polyamide column, mobile phase be respectively 30vol% ethanol,
50vol% ethanol, 80vol% ethanol, first using 3000mL 30vol% ethanol elutions, then using 6000mL 50vol% second
Alcohol elutes, and finally using 4000mL 80vol% ethanol elutions, collects the eluent of 80vol% ethanol, is evaporated under reduced pressure to without second
Alcohol, 200mL absolute ethers are then added, filtering, filtrate are evaporated, obtain solid, 2h is dried in vacuo, produces.
A kind of method for preparing a kind of skin conditioning composition described in embodiment any one of 1-9 of embodiment 10., institute
It is as follows to state step：
Each component is weighed by weight, the proud peace radix scutellariae root extract of skin is added in butanediol, after stirring, first
Sequentially add Bacillus acidi lactici/pear juice tunning filtrate, amur cork tree bark extract, it is to be mixed uniformly after, after being heated to 40 DEG C, according to
Secondary addition surplus materials, after stirring, is cooled to room temperature.
The present invention is expanded on further with reference to specific embodiment.
Embodiment 1：The present embodiment provides a kind of skin conditioning composition, in parts by weight, the skin conditioning composition
Thing includes：
Amur cork tree bark extract：The extracting method of the amur cork tree bark extract is as follows：
500g amur cork tree barks are taken, are ground to 100-200 mesh, 4000mL distilled water/alcohol mixeding liquid is added, is heated to reflux
12h, room temperature is cooled to, filtered, collect filtrate, add NaOH to pH=11, then added 500mL toluene, liquid separation, collect toluene
Layer, adds 15mL polyphosphoric acids, and 100mL distilled water is added after stirring, after standing 2 hours, liquid separation, removes toluene layer, subtracts
Pressure distillation, 100mL is concentrated into by liquor capacity, is filtered, and is collected solid, is added 800mL distilled water, be heated to reflux 45min, so
50mL saturation sodium sulfide solutions are added afterwards, are continued the 1h that flows back, are subsequently cooled to room temperature, add 100mL saturation calcium chloride solutions,
30min is stirred, filtering, collects filtrate, hydrochloric acid is added to pH=6, reuses Rotary Evaporators and solution is concentrated into 50mL, mistake
Filter, solid is collected, be dried in vacuo 12h, produce.
In the distilled water/alcohol mixeding liquid, the volume ratio of distilled water and ethanol is 8：2.
Chinese glutinous rehmannia extract：The extracting method of the Chinese glutinous rehmannia extract is as follows：
40 parts of Chinese glutinous rehmannia are taken, four parts is equally divided into, washing, after drying, is ground to 100-200 mesh, is separately added into 50 parts
Methanol, it is heated to reflux, merges the extract solution of four times, methanol is then recovered under reduced pressure, obtained methanol extract is diluted with water, selects
Extracting n-butyl alcohol three times, takes n-butanol layer, and after being then concentrated under reduced pressure, add diethyl ether dissolving, takes brown color insoluble matter.To brown color not
Molten thing carries out column chromatography for separation, and stationary phase uses activated carbon diatomite, is eluted by the use of water as mobile phase, collects the elution of mobile phase
Product, 4h is dried in vacuo, dried object is obtained, produces.
White willow bark extract：The extracting method of the white willow bark extract is as follows：
500g white willow barks are taken, washing, after drying, 100-200 mesh is ground to, adds 1000mL distilled water and 5g Vitamin Cs
Acid, the method continuously extracted using ultrasonic adverse current, after being extracted 2 hours under conditions of 40-70 DEG C, centrifugation, filtrate is taken, it is rotated
Filtrate is evaporated by evaporimeter, obtains brown solid；Column chromatography for separation is carried out to brown solid, stationary phase uses neutral alumina,
First eluted with the first mobile phase, after elution completely, reuse the elution of the second mobile phase, collect the elution production of the second mobile phase
Thing, 4h is dried in vacuo, obtains dried object；Dried object is dissolved in 30mL 20wt% hydrochloric acid, after stirring 2 hours, adds 5mL
68wt% nitric acid, after 30min, after 5 DEG C are cooled in ice-water bath, collect solid, after deionized water is washed, dry,
Produce.
First mobile phase is by chloroform and methanol with volume ratio 6：1 is mixed to get, and the second mobile phase is by chloroform and methanol with body
Product ratio 1：1 is mixed to get.
Bacillus acidi lactici/pear juice tunning filtrate：The preparation process of the Bacillus acidi lactici/pear juice tunning filtrate is such as
Under：
(1) by European pear, clean, peeling, defibrination；1000g defibrinations are taken, add distilled water water, control the sugar content of defibrination
10%；
(2) dry ferment is added into composite fruit juice fermentation raw material by 0.02wt% additions, fermented under the conditions of 28 DEG C~30 DEG C
15 days obtained one time fermentation liquid, controlling pH is between 2g/L~5g/L；
(3) secondary fermentation：Saccharomycete, acetobacter aceti, lactobacillus acidophilus and lactobacillus species viable count >=0.8 ×
107Cfu/mL, by volume saccharomycete：Acetobacter aceti：Lactobacillus acidophilus：Bacillus acidi lactici=1：1：1：4 are made mixed bacteria；
Mixed bacteria is added into one time fermentation liquid by 8% inoculum concentration, ferment 1~3 day obtained composite fruit juice zymotic fluid under the conditions of 28 DEG C, hair
Zymotic fluid total acid is controlled in more than 4.5g/dL；
(4) above-mentioned zymotic fluid is taken, is filtered by vacuum, stood using diatomite, take filtrate.
Neem leaf extract：The extracting method of the neem leaf extract is as follows：
Azadirachta leaf 300g is taken, is cleaned, chopping, is added 2000mL absolute ethyl alcohols, 24h is soaked at 4 DEG C, is flowed back at 50 DEG C
Extraction 3 hours, filtering, filtrate is collected, is concentrated under reduced pressure into 200mL, added 200mL chloroforms, liquid separation, collect chloroform extract,
200mL 4wt%NaOH are added, liquid separation, collect water layer, hydrochloric acid is then added to pH=6, adds 200mL chloroforms, liquid separation, receive
Collect chloroform layer, with Rotary Evaporators solvent evaporated, obtain solid；
Solid is dispersed in water, adds 0.01g glucose and 0.2g/L spine root peroxidase and 5mL 20wt%
Hydrogen peroxide, stirring reaction is after 2 hours at 40 DEG C, filtering, after 10mL ether washs 3 times, dries, produces.
Radix scutellariae root extract：The extracting method of the radix scutellariae root extract is as follows：
500g scutellariae,radixs are taken, are ground to 100-200 mesh, add 1000mL distilled water, the temperature of distilled water is 60-70
DEG C, after soaking 24 hours, filtering, taking precipitate；300mL benzene and 0.05g ferrocene is added in sediment, is heated to reflux 3
After hour, benzene liquid is collected, 100mL is concentrated into after cooling, add 100mL distilled water, collected distillation water layer, use Rotary Evaporators
Solvent evaporated, obtain solid, then using column chromatography, stationary phase is polyamide column, mobile phase be respectively 30vol% ethanol,
50vol% ethanol, 80vol% ethanol, first using 3000mL 30vol% ethanol elutions, then using 6000mL 50vol% second
Alcohol elutes, and finally using 4000mL 80vol% ethanol elutions, collects the eluent of 80vol% ethanol, is evaporated under reduced pressure to without second
Alcohol, 200mL absolute ethers are then added, filtering, filtrate are evaporated, obtain solid, 2h is dried in vacuo, produces.
Houttuynia extract：The extracting method of the houttuynia extract is as follows：Fresh cordate houttuynia is taken, it is rapid to clean, cut
Section, add the water of 10 times of weight of cordate houttuynia piece, and the Ginger Polysaccharide of 0.05 times of weight of cordate houttuynia, soak 1h, decoct 2 hours, filtering,
The dregs of a decoction add the water of 8 times of weight of medicinal material, decoct 1 hour, and decoction filters out, and merge decoction liquor twice, are concentrated into 2 times of weights of cordate houttuynia
The boil liquid of amount, ethanol is added the percent by volume of alcohol content is stirred, is stood, filtering is stand-by up to 75%.
Wild soybean albumen：The Wild soybean albumen purchase is liked from the strong peace in the U.S..
Skin is proud peaceful：The proud peaceful purchase of the skin is former from Japanese woods.
The second aspect of the present embodiment provides a kind of skin conditioning agent, and it is prepared by a kind of above-mentioned skin conditioning composition
Obtain, it is characterised in that the preparation process is as follows：
Each component is weighed by weight, the proud peace radix scutellariae of skin is added in butanediol, after stirring, is first once added
Foreign pear juice tunning filtrate, phellodendron extract, it is to be mixed uniformly after, after being heated to 40 DEG C, sequentially add surplus materials, stir
After mixing uniformly, room temperature is cooled to.
Embodiment 2：Difference with embodiment 1 is that in parts by weight, the skin conditioning composition includes：
Embodiment 3：Difference with embodiment 1 is that in parts by weight, the skin conditioning composition includes：
Embodiment 4：Difference with embodiment 1 is that the amur cork tree bark extract is commercially available, buys the prosperous life from Shaanxi
Thing Technology Co., Ltd..
Embodiment 5：Difference with embodiment 1 is that the radix scutellariae root extract is commercially available, buys the prosperous biology from Shaanxi
Technology Co., Ltd..
Embodiment 6：Difference with embodiment 1 is that the neem leaf extract is commercially available, buys real from Shanghai Ao highland barleys
Industry Co., Ltd.
Embodiment 7：Difference with embodiment 1 is that the extracting method of the white willow bark extract is as follows：Take 500g white
Willow bark, washing, after drying, 100-200 mesh is ground to, adds 1000mL distilled water, the side continuously extracted using ultrasonic adverse current
Method, after being extracted 2 hours under conditions of 40-70 DEG C, centrifugation, filtrate is taken, filtrate is evaporated by rotated evaporimeter, obtains brown and consolidates
Body；Column chromatography for separation is carried out to brown solid, stationary phase uses neutral alumina, is first eluted with the first mobile phase, waits to have eluted
Quan Hou, the elution of the second mobile phase is reused, collect the eluted product of the second mobile phase, be dried in vacuo 4h, obtain dried object；Will be dry
Dry thing is dissolved in 30mL 20wt% hydrochloric acid, after stirring 2 hours, adds 5mL 68wt% nitric acid, after 30min, in frozen water
After 5 DEG C are cooled in bath, solid is collected, after deionized water is washed, dries, produces.
Embodiment 8：Difference with embodiment 1 is that the extracting method of the neem leaf extract is as follows：Take azadirachta
Leaf 300g, clean, chopping, add 2000mL absolute ethyl alcohols, soak 24h at 4 DEG C, refluxing extraction 3 hours at 50 DEG C, filtering,
Filtrate is collected, is concentrated under reduced pressure into 200mL, 200mL chloroforms is added, liquid separation, collects chloroform extract, add 200mL 4wt%
NaOH, liquid separation, water layer is collected, then add hydrochloric acid to pH=6, add 200mL chloroforms, liquid separation, chloroform layer is collected, with rotation
Evaporimeter solvent evaporated, obtains solid.
Solid is dispersed in water, adds 0.01g glucose and 5mL 20wt% hydrogen peroxide, is stirred at 40 DEG C anti-
After answering 2 hours, filtering, after 10mL ether washs 3 times, dry, produce.
Embodiment 9：Difference with embodiment 1 is that the extracting method of the neem leaf extract is as follows：
Azadirachta leaf 300g is taken, is cleaned, chopping, is added 2000mL absolute ethyl alcohols, 24h is soaked at 4 DEG C, is flowed back at 50 DEG C
Extraction 3 hours, filtering, filtrate is collected, is concentrated under reduced pressure into 200mL, added 200mL chloroforms, liquid separation, collect chloroform extract,
200mL 4wt%NaOH are added, liquid separation, collect water layer, hydrochloric acid is then added to pH=6, adds 200mL chloroforms, liquid separation, receive
Collect chloroform layer, with Rotary Evaporators solvent evaporated, obtain solid；
Solid is dispersed in water, 0.01g glucose and 0.2g/L spine root peroxidase is added, is stirred at 40 DEG C
After reaction 2 hours, filtering, after 10mL ether washs 3 times, dry, produce.
Embodiment 10：Difference with embodiment 1 is that the extracting method of the radix scutellariae root extract is as follows：
500g scutellariae,radixs are taken, are ground to 100-200 mesh, add 1000mL distilled water, the temperature of distilled water is 60-70
DEG C, after soaking 24 hours, filtering, taking precipitate；300mL benzene is added in sediment, after being heated to reflux 3 hours, collects benzene
Liquid, 100mL is concentrated into after cooling, adds 100mL distilled water, collected distillation water layer, with Rotary Evaporators solvent evaporated, obtain
Solid, then using column chromatography, stationary phase is polyamide column, mobile phase be respectively 30vol% ethanol, 50vol% ethanol,
80vol% ethanol, first using 3000mL 30vol% ethanol elutions, then using 6000mL 50vol% ethanol elutions, finally
Using 4000mL 80vol% ethanol elutions, the eluent of 80vol% ethanol is collected, is evaporated under reduced pressure to without ethanol, then adds
200mL absolute ethers, filtering, filtrate is evaporated, and obtains solid, is dried in vacuo 2h, is produced.
Embodiment 11：Difference with embodiment 1 is, the step of the method for preparing a kind of above-mentioned skin conditioning composition
It is rapid as follows：Each component is weighed by weight, and each material is added in butanediol, after being heated to 40 DEG C, after stirring, cooling
To room temperature.
Test：
The application method of the skin conditioning composition of the present invention is to clean up the face of user, using normal
The skin care step of rule：The first step is first to wipe toner；Second step is to wipe face cream, and the 3rd step smears the skin condition of the present invention
Composition.
Wherein, the mildy wash of the trier, toner, face cream are same.
Test 1, heavy metal analysis experiment
Shanghai Wei Pu Chemical Engineering Technologies Services Co., Ltd is entrusted, heavy metal in embodiment 1-11 skin conditioning agent is contained
Amount is detected, and test result is shown in Table 1.
Table 1：
| Embodiment | Mercury (mg/kg) | Lead (mg/kg) | Arsenic (mg/kg) | Cadmium (mg/kg) |
| Example 1 | <1 | <1 | <1 | <2 |
| Example 2 | <1 | <1 | <1 | <2 |
| Example 3 | <1 | <1 | <1 | <2 |
| Example 4 | <1 | <1 | <1 | <2 |
| Example 5 | <1 | <1 | <1 | <2 |
| Example 6 | <1 | <1 | <1 | <2 |
| Example 7 | <1 | <1 | <1 | <2 |
| Example 8 | <1 | <1 | <1 | <2 |
| Example 9 | <1 | <1 | <1 | <2 |
| Example 10 | <1 | <1 | <1 | <2 |
| Example 11 | <1 | <1 | <1 | <2 |
The heavy metal analysis result of the pore composition that compacts of all embodiments is qualified.
2nd, skin conditioning agent carries out clinical effect trial.
First, subject enrollment standard：
(1) men and women is unlimited, coordinates product beta test requirement；(2) face has obvious acne, small pox and enlarged pores；(3) energy
Good compatibility test, good daily life system time and eating habit can be kept in the trial employment period.
2nd, given the test agent, sample application method and time
Given the test agent is embodiment 1-11 skin conditioning agent and skin conditioning agent is not used in embodiment 12, and specification is every
5mL；
The usage time of sample：Usage time is two weeks, 2 times a day, once in the morning and once at night；
3rd, the course for the treatment of is tested
Time on probation is two weeks, is observed in three times, respectively treat the 1st, treat 1 week after and treatment end day.
Time on probation is：Morning 8:30-10:00, afternoon 5:03-6:30.
4th, result on trial is analyzed
Trial period is two weeks.
Person on probation must not be less than 1000 people.
A total of 1200 people participates in the on probation of cosmetics (added with the pore composition that compacts).12 groups are randomly divided into, respectively
Use embodiment 1-12 cosmetics.During subject's Evaluation product, do not occur pruitus, shouting pain, redness etc..
Method of testing and effect standard
Test (1) Evaluation of Synthetic Effect of Holistic pore compacts situation (visually observing), skin lesion degree, skin lesion distribution, symptom, sign
Total mark calculate curative effect rate.Skin lesion counts * before curative effect=(skin lesion counts after skin lesion counting-treatment before treatment)/treatment
100%.Cure：Visually observe and can't see pore；It is effective：Pore compacts 80%, symptom mitigation；Effectively：Pore compacts 20%~
60%, symptom improves；It is invalid：Pore compacts<20%, clinical symptoms do not improve.Total effective rate=cure rate+obvious effective rate+effectively
Rate.Treatment time is 2 weeks.
Test result is shown in Table 2
Table 2：Compact pore clinical test results
| Embodiment | Cure | It is effective | Effectively | It is invalid |
| Example 1 | 90% | 5% | 5% | 0% |
| Example 2 | 87% | 7% | 6% | 0% |
| Example 3 | 87% | 8% | 5% | 0% |
| Example 4 | 82% | 8% | 10% | 0% |
| Example 5 | 77% | 5% | 7% | 11% |
| Example 6 | 80% | 5% | 5% | 10% |
| Example 7 | 60% | 11% | 10% | 19% |
| Example 8 | 64% | 9% | 10% | 17% |
| Example 9 | 73% | 6% | 5% | 16% |
| Example 10 | 55% | 7% | 16% | 22% |
| Example 11 | 50% | 6% | 17% | 27% |
| Example 12 | 3% | 7% | 10% | 70% |
Test (2) reference《New Chinese medicine guideline of clinical investigations》, Evaluation of Synthetic Effect of Holistic is with skin lesion degree, skin lesion point
Cloth, symptom, the total mark of sign calculate curative effect rate.Before curative effect=(skin lesion counts after skin lesion counting-treatment before treatment)/treatment
Skin lesion counts * 100%.Cure：Skin lesion regression more than 90%, only a little pigmentation；It is effective：Skin lesion regression 60%~
90%, symptom mitigation；Effectively：Skin lesion regression 20%~60%, symptom improves；It is invalid：Skin lesion is disappeared<20%, clinical symptoms are not
Improve.Total effective rate=cure rate+obvious effective rate+effective percentage.Treatment time is 2 weeks.
Test result is shown in Table 3
Table 3：Test (2) result of the test
Test (3), moisture retention effect assessment
Test philosophy：According to composition moisture absorption, the difference of performance of keeping humidity, active force of the different moisturizing agent molecules to hydrone
Difference, it is different with the ability of moisture is kept to absorb moisture.Oil content has sealing process to moisture, can prevent scattering and disappearing for moisture.Moisture absorption
Active force is big, strong to water molecules power, absorbs and keeps that the amount of moisture is also bigger, closure is good, moisture loss
Also it is few.The biomaterial of cuticula, epidermis etc. is imitated using adhesive tape, cosmetics are coated with adhesive tape, simulates practical application situation.
Sample is placed under conditions of constant humidity after certain time, is weighed and is put front and rear of poor quality, obtains the loss of sample size, can calculate
The effect of moisturizing ingredient moisturizing.It is shown in Table 4.
Moisturizing rate calculation formula：Moisturizing rate=(M2–M0)/(M1–M0) × 100%
In formula：
M0：Hollow plate quality/g；
M1：Glass sheet quality/g after sample-adding；
M2：Quality/g after some hours is placed in drier；
Test its relative humidity be 60% when 4h and 8h in moisturizing rate；
Table 4
Test (4), bacteriostasis property evaluation
Due to air pollutions such as hazes so that in air, Escherichia coli, staphylococcus aureus, propionibacterium acnes connect
Tactile skin easily cause skin acne, it is sensitive phenomena such as.
Bacteriostasis property evaluation reference Chinese Patent Application No. in the application：CN201510395607.9.
Odontothrips loti：Corresponding culture medium, 121 DEG C of high pressure steam sterilization, 20min are configured according to different strains testeds.Make
Solid medium flat board, each culture dish about pours into 20mL culture mediums, and after culture medium solidifying, 100 μ L bacterium solutions are taken with liquid-transfering gun
(bacteria suspension for having been subjected to Maxwell turbidimetry for Determination concentration) is instilled in culture dish, and using spread plate, bacteria suspension is uniformly applied
It is distributed on solid medium.Media surface directly vertically put Oxford cup (internal diameter 6mm, external diameter 8mm, high 10mm circle
Tubule), gently pressurize, it is contacted tight with culture medium.Each culture dish places three Oxford cups.Add in each Oxford cup
Enter 200 μ L testing samples, the culture dish of bacterium is gently placed in bacteriological incubator or so 37 DEG C of cultures, 16 hours.It is shown in Table 5.
Note：Antibacterial circle diameter >=30mm has been judged to very good bacteriostasis, antibacterial circle diameter 20mm for extremely sensitive " ++++"
≤ bacteriostatic diameter<30mm has been judged to bacteriostasis, 15mm≤bacteriostatic diameter for extremely sensitive " +++ "<20mm is Gao Min " ++ "；10mm
≤ bacteriostatic diameter<15mm quick "+" in being；Bacteriostatic diameter<10mm is invalid, is judged to no bacteriostasis.Wherein, the bigger suppression of inhibition zone
Bacterium effect is better.
The fungistatic effect evaluation table of table 5
The present invention product for Escherichia coli, staphylococcus aureus, propionibacterium acnes have preferably it is antibacterial
Effect.
Test (5), physical and chemical index test
Sensory testing：The character of product is visually observed, sees if there is exception；
Resistance to Thermal test：Sample is put into 24h in the electro-heating standing-temperature cultivator of (40 ± 1) DEG C, seen whether after recovering room temperature
There is phenomena such as thinning, discoloration, layering and firmness change, with the heat resistance of judgement sample；
Low temperature resistant test：Sample is put into 24h in the refrigerator of (- 5~-10) DEG C ± 1 DEG C, seen whether after recovering room temperature
Phenomena such as thinning, discoloration, layering and firmness change, with the cold tolerance of judgement sample；
Centrifugal test：Sample is placed in a centrifuge, with (2000~4000) r/min speed test 30min, observes sample
The separation of product, layering situation.
The physical and chemical index result of table 3
Foregoing example is merely illustrative, some features of the feature for explaining the disclosure.Appended claim
It is intended to require the scope as wide as possible being contemplated that, and embodiments as presented herein is only according to all possible embodiment
Combination selection embodiment explanation.Therefore, the purpose of applicant is appended claim not by the explanation present invention
Feature example selectional restriction.And the progress in science and technology will not formed due to the inaccuracy of language performance and not
The possible equivalent or son being presently considered are replaced, and these changes should also be interpreted by appended in the conceived case
Claim covers.
Claims (10)
1. a kind of skin conditioning composition, it is characterised in that the skin conditioning composition includes：
Butanediol, Bacillus acidi lactici/pear juice tunning filtrate, amur cork tree bark extract, white willow bark extract, Chinese Radix Rehmanniae
Extract, neem leaf extract, radix scutellariae root extract, houttuynia extract, Wild soybean albumen, Pi Aoning.
A kind of 2. skin conditioning composition described in claim 1, it is characterised in that in parts by weight, the skin condition
Composition includes：
A kind of 3. skin conditioning composition described in claim 1, it is characterised in that in parts by weight, the skin condition
Composition includes：
A kind of 4. skin conditioning composition described in claim 1, it is characterised in that in parts by weight, the skin condition
Composition includes：
A kind of 5. skin conditioning composition described in claim 1, it is characterised in that the extraction side of the amur cork tree bark extract
Method is as follows：
500g amur cork tree barks are taken, are ground to 100-200 mesh, 4000mL distilled water/alcohol mixeding liquid is added, is heated to reflux 12h, it is cold
But to room temperature, filtering, filtrate is collected, adds NaOH to pH=11, then added 500mL toluene, liquid separation, collect toluene layer, then
15mL polyphosphoric acids is added, 100mL distilled water is added after stirring, after standing 2 hours, liquid separation removes toluene layer, and decompression is steamed
Evaporate, liquor capacity is concentrated into 100mL, filter, collect solid, add 800mL distilled water, be heated to reflux 45min, Ran Houjia
Enter 50mL saturation sodium sulfide solutions, continue the 1h that flows back, be subsequently cooled to room temperature, add 100mL saturation calcium chloride solutions, stir
30min, filtering, filtrate is collected, add hydrochloric acid to pH=6, reuse Rotary Evaporators and solution is concentrated into 50mL, filter, receive
Collect solid, be dried in vacuo 12h, produce.
A kind of 6. skin conditioning composition described in claim 1, it is characterised in that the extraction side of the white willow bark extract
Method is as follows：
500g white willow barks are taken, washing, after drying, 100-200 mesh is ground to, adds 1000mL distilled water and 5g ascorbic acid,
The method continuously extracted using ultrasonic adverse current, after being extracted 2 hours under conditions of 40-70 DEG C, centrifugation, take filtrate, rotated steaming
Filtrate is evaporated by hair instrument, obtains brown solid；Column chromatography for separation is carried out to brown solid, stationary phase uses neutral alumina, first
Eluted with the first mobile phase, after elution completely, reuse the elution of the second mobile phase, collect the eluted product of the second mobile phase,
4h is dried in vacuo, obtains dried object；Dried object is dissolved in 30mL 20wt% hydrochloric acid, after stirring 2 hours, adds 5mL's
68wt% nitric acid, after 30min, after 5 DEG C are cooled in ice-water bath, solid is collected, after deionized water is washed, is dried, i.e.,
.
A kind of 7. skin conditioning composition described in claim 1, it is characterised in that the Bacillus acidi lactici/pear juice tunning
The preparation process of filtrate is as follows：
(1) by European pear, clean, peeling, defibrination；Take 1000g defibrinations, add distilled water water, control the sugar content of defibrination 8~
18%；
(2) dry ferment is added into composite fruit juice fermentation raw material by 0.01%~0.05wt% additions, under the conditions of 28 DEG C~30 DEG C
Ferment 9~17 days obtained one time fermentation liquid, and controlling pH is between 2g/L~5g/L；
(3) secondary fermentation：Saccharomycete, acetobacter aceti, lactobacillus acidophilus and lactobacillus species viable count are >=0.8 × 107cfu/
ML, by volume saccharomycete：Acetobacter aceti：Lactobacillus acidophilus：Bacillus acidi lactici=1：1：1：4 are made mixed bacteria；Connect by 8%
Mixed bacteria is added one time fermentation liquid by kind amount, and ferment 1~3 day obtained composite fruit juice zymotic fluid under the conditions of 28 DEG C, and zymotic fluid is total
Acid control is in more than 4.5g/dL；
(4) above-mentioned zymotic fluid is taken, is filtered by vacuum, stood using diatomite, take filtrate.
A kind of 8. skin conditioning composition described in claim 1, it is characterised in that the extraction side of the neem leaf extract
Method is as follows：
Azadirachta leaf 300g is taken, is cleaned, chopping, is added 2000mL absolute ethyl alcohols, soak 24h at 4 DEG C, refluxing extraction at 50 DEG C
3 hours, filtering, filtrate is collected, is concentrated under reduced pressure into 200mL, added 200mL chloroforms, liquid separation, collect chloroform extract, add
200mL 4wt%NaOH, liquid separation, water layer is collected, then add hydrochloric acid to pH=6, add 200mL chloroforms, liquid separation, collect chlorine
Imitative layer, with Rotary Evaporators solvent evaporated, obtains solid；
Solid is dispersed in water, adds 0.01g glucose and 0.2g/L spine root peroxidase and 5mL 20wt% peroxides
Change hydrogen, stirring reaction is after 2 hours at 40 DEG C, filtering, after 10mL ether washs 3 times, dries, produces.
A kind of 9. skin conditioning composition described in claim 1, it is characterised in that the extracting method of the radix scutellariae root extract
It is as follows：
500g scutellariae,radixs are taken, are ground to 100-200 mesh, add 1000mL distilled water, the temperature of distilled water is 60-70 DEG C, leaching
Bubble is after 24 hours, filtering, taking precipitate；300mL benzene and 0.05g ferrocene is added in sediment, is heated to reflux 3 hours
Afterwards, benzene liquid is collected, 100mL is concentrated into after cooling, adds 100mL distilled water, distillation water layer is collected, is evaporated with Rotary Evaporators
Solvent, solid is obtained, then using column chromatography, stationary phase is polyamide column, and mobile phase is respectively 30vol% ethanol, 50vol%
Ethanol, 80vol% ethanol, first using 3000mL 30vol% ethanol elutions, then using 6000mL 50vol% ethanol elutions,
Finally use 4000mL 80vol% ethanol elutions, collect the eluent of 80vol% ethanol, be evaporated under reduced pressure to without ethanol, then
200mL absolute ethers are added, filtering, filtrate are evaporated, obtain solid, 2h is dried in vacuo, produces.
10. a kind of skin conditioning agent, it is prepared as a kind of skin conditioning composition described in claim any one of 1-9,
Characterized in that, the preparation process is as follows：
Each component is weighed by weight, the proud peace radix scutellariae root extract of skin is added in butanediol, after stirring, first successively
Add Bacillus acidi lactici/pear juice tunning filtrate, amur cork tree bark extract, it is to be mixed uniformly after, after being heated to 40 DEG C, successively plus
Enter surplus materials, after stirring, be cooled to room temperature.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109260119A (en) * | 2018-11-27 | 2019-01-25 | 大连民族大学 | A kind of anti-acne repairs facial mask and preparation method thereof |
| CN111534455A (en) * | 2020-04-02 | 2020-08-14 | 广州栋方生物科技股份有限公司 | Preparation of lactobacillus sporolysis product and application of lactobacillus sporolysis product in cosmetics |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1556101A (en) * | 2003-12-31 | 2004-12-22 | 中国药科大学 | Extraction technology of Hanbaicalein, medicinal composition and preparation technology of medicine |
| CN102961364A (en) * | 2012-12-12 | 2013-03-13 | 百岳特生物科技（上海）有限公司 | Sensitive and color-changing anti-acne repairing diaphragm |
| CN104861013A (en) * | 2015-06-15 | 2015-08-26 | 白心亮 | Method for extracting salicin from white willow bark |
| CN105770160A (en) * | 2016-04-15 | 2016-07-20 | 广州宝生堂化妆品有限公司 | Skin care composition with acne removing function |
| CN106821952A (en) * | 2017-04-05 | 2017-06-13 | 广州市胜梅化妆品有限公司 | It is the dead skin condensation of dispelling of raw material to use plant extraction liquid |
-
2017
- 2017-10-18 CN CN201710970524.7A patent/CN107595706A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1556101A (en) * | 2003-12-31 | 2004-12-22 | 中国药科大学 | Extraction technology of Hanbaicalein, medicinal composition and preparation technology of medicine |
| CN102961364A (en) * | 2012-12-12 | 2013-03-13 | 百岳特生物科技（上海）有限公司 | Sensitive and color-changing anti-acne repairing diaphragm |
| CN104861013A (en) * | 2015-06-15 | 2015-08-26 | 白心亮 | Method for extracting salicin from white willow bark |
| CN105770160A (en) * | 2016-04-15 | 2016-07-20 | 广州宝生堂化妆品有限公司 | Skin care composition with acne removing function |
| CN106821952A (en) * | 2017-04-05 | 2017-06-13 | 广州市胜梅化妆品有限公司 | It is the dead skin condensation of dispelling of raw material to use plant extraction liquid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109260119A (en) * | 2018-11-27 | 2019-01-25 | 大连民族大学 | A kind of anti-acne repairs facial mask and preparation method thereof |
| CN111534455A (en) * | 2020-04-02 | 2020-08-14 | 广州栋方生物科技股份有限公司 | Preparation of lactobacillus sporolysis product and application of lactobacillus sporolysis product in cosmetics |
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Effective date of registration: 20190116 Address after: 214400 Room 301, Building 48, Sanyuanfang Lane, Jiangyin City, Wuxi City, Jiangsu Province Applicant after: Chen Xiao Address before: 201599 Block C, Room 2083, Building 218 Xianju Road, Zhujing Town, Jinshan District, Shanghai Applicant before: (Shanghai) International Trading Co., Ltd. |
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Application publication date: 20180119 |