CN107594260A - A kind of method that continuous submerged fermentation prepares tremella beverage - Google Patents
A kind of method that continuous submerged fermentation prepares tremella beverage Download PDFInfo
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Abstract
The invention discloses a kind of method that continuous submerged fermentation prepares tremella beverage, by white fungus mycelia or fructification, seed liquor culture is carried out under additional electromagnetic field auxiliary, then by seed liquor being fixed of thalline and its band is made to be magnetic, by the white fungus thalline of immobilization, under the auxiliary of high-pressure pulse electric carrying out submerged fermentation is prepared into tremella beverage.There is advantages below by the preparation-obtained tremella beverage of the present invention：White fungus extracts active ingredients are abundant, and the production cycle greatly reduces；Magnetic immobilized thalline can repeatedly be fermented, and be advantageous to the separation and purifying of product, reduce the burden of downstream technique；The problems such as utilization rate of active components is low, microbiological contamination is more, fermentation period is long in current tremella beverage preparation technology is improved, technical foundation is provided for later diversification production.
Description
Technical field
The invention belongs to fermentation engineering field, and tremella beverage method is prepared more particularly to a kind of fermentation technique.
Background technology
White fungus is a kind of medicine-food two-purpose bacterium with very high economic value, is the traditional rare excellent tonic product in China, existing
The effect of tonifying spleen is whetted the appetite, there is QI invigorating gut purge, nourishing Yin and moistening lung again.Body immunity can be strengthened, tumour trouble can be strengthened again
Tolerance of the person to Radiotherapy chemotherapy.White fungus has the function that enriching yin rich in natural plant colloid, additional its, is to take for a long time
Good moisturizing food.The multiple efficacies of white fungus make scholar carry out many research and development to it, mainly in medicine, health care
Product, edible etc..Therefore, there are good market prospects to prepare the deep processed products such as beverage using white fungus.But at present
The edible fungi beverage in market uses using fruit body of edible fungi as raw material more, after leaching juice or squeezing, obtains having containing part
Effect composition juice be diluted again, adds auxiliary and condiment after be made, this technique is complex, growth cycle grow, and
The active ingredient of edible mushroom can not fully be extracted.Therefore need to develop the high production method of a kind of high efficiency, material utilization
Carry out the preparation of edible fungi beverage.
Magnetic field can influence the normal physiological function of cell, and prepare culture medium using magnetized solution is carried out to yeast cells
Culture, the yeast count showed increased compared with unused magnetized solution is cultivated；It is red using relatively low magnetic induction intensity culture
Beans China fir cell, it is found that its cell viability significantly improves, cometabolism has strengthened, it is seen that suitable magnetic field has positive make to cell
With.But current magnetic field assisted fermentation exist the production cycle it is long the problem of.It is a kind of disclosed in patent of invention CN201310025724.7
High viable bacteria content complex microorganism granular fertilizer and preparation method thereof, reach trophosome number most in liquid deep layer fermenting using microorganism
When more, material is imported in the magnetic field environment of 3724 Gausses and tamed 13 hours, substantially increases anti-adversity and the guarantor of beneficial bacterium
Deposit the time.
High-pressure pulse electric is a kind of new foods processing technique in recent years, and it is that the sample solution of liquid is served as into electricity
Xie Zhi, two electrodes to be insulated in the electric field with container can produce high-tension current, have electric pulse effect, so as to be carried out to food
Continuous non-thermal technology processing, in terms of being currently used primarily in food sterilization, managed using the material of high-voltage pulse electric field technology extraction
Change structure, mouthfeel, flavor etc. to be not easy to be changed, and there is the characteristics of Extracting temperature is low, and the time is short, power consumption is low and efficient.
In recent years, both at home and abroad using the active ingredient in high-voltage pulse electric field technology extraction food, such as polysaccharide, protein, DNA, solvable
Property calcium, pigment and vitamin etc..A kind of composite fungi amylose and combinations thereof disclosed in patent of invention CN201610169995.3
Functional food, raw material is handled using high-pressure pulse electric, improve polysaccharide yield.Research is found, in certain limit
The impulse electric field of electric-field intensity can change membrane passage, be advantageous to absorption of the cell to external substance and intracellular
Tolerant oozes out, but prolonged high voltage electric field effect can be cell rupture death, and this is unfavorable to fermentation.
Immobilized cell technology is used for fermentation technique earliest, and the technology protects cells from fermentation process to a certain extent
In mechanical agitation shear property model, increase cell concentration and ferment strength, to realize that cell separates offer facility with product；Cell
Cell density is big after immobilization, reaction speed is fast, resistance to malicious ability is strong, can realize continuous operation, is greatly improved production capacity.Hair
A kind of carrier immobilized enzyme of temperature control phase-change material micro-capsule and preparation method thereof disclosed in bright patent CN201610552601.2,
Solution conventional carriers immobilised enzymes applicable working temperature narrow range and storage stability, heat endurance and cyclical stability are poor to ask
Topic, the stability and activity for making institute's immobilized enzyme are significantly improved.
The content of the invention
Present invention aim to address current tremella beverage prepare existing for complex process, utilization rate of active components is low etc. to ask
Topic, there is provided it is a kind of by continuous submerged fermentation, high-pressure pulse electric auxiliary deep layer hair is coordinated with magnetic field, microcapsules immobilization technology
The method that ferment produces tremella beverage.
Technical scheme is as follows：A kind of method that continuous submerged fermentation prepares tremella beverage, comprises the following steps：
1. a kind of method that continuous submerged fermentation prepares tremella beverage, it is characterised in that preparation method step includes：
F1：Take white fungus mycelia or fructification, after cleaning, drying to moisture are 6 ~ 8%, be crushed to the acquisition of 100 ~ 120 mesh
White fungus powder, white fungus powder and water are pressed into solid-liquid ratio 1：（40~60）It is well mixed, seed material is obtained after sterilizing；
F2：By seed material obtained by F1 aseptically by weight percentage 15 ~ 20% inoculum concentration be seeded to it is compound after sterilizing
In amino acid media, it is subsequently placed on shaking table and is passed through electromagnetic field and carry out culture 24 ~ 48 hours, 23 ~ 25 DEG C of cultivation temperature, obtains
Seed liquor；
F3：The sediment that the centrifugation of F2 seed liquors is obtained, aseptically is well mixed to obtain with magnetic sodium alginate soln
Mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out 20 ~ 30min of solidification, magnetic is obtained and carries thalline alginic acid
Calcium coagulates pearl；Magnetic calcium alginate is coagulated into pearl with water washing is distilled 2 ~ 3 times, then with the wall material solution after sterilizing aseptically
Mix, 15 ~ 30min is vibrated under 200 ~ 230rpm rotating speeds and carries out film formation reaction, obtain magnetic and carry thalline microcapsules；
Described magnetic sodium alginate soln, it is that magnetic material is added in 20 ~ 25mg/ml sodium alginate solns to be well mixed
It is prepared；The wall material solution concentration is 0.8 ~ 1.2mg/ml；Described calcium chloride solution concentration is 18 ~ 22mg/ml；It is described
It is 1 to solidify mixed liquor with calcium chloride solution volume ratio：（1.5~2）；The magnetic calcium alginate coagulates pearl and wall material solution volume ratio
For 1：（5~10）；
The magnetic material is some magnetic particles, has the material that saturation magnetic intensity is low but stability is good, it is micro- to may be selected as magnetic
Grain only has a few, mainly there is metal oxide γ-Fe2O3, MeFe2O4（Me=Co, Mn, Ni）, Fe3O4, W metal, Co,
Fe and its alloy Fe-Co, Ni-Fe etc.；The present invention uses Fe3O4；
The wall material solution, it is that complex reaction can occur with anionic polyelectrolytes such as sodium alginates in aqueous, is formed
The polymeric membrane being made up of polyelectrolyte complex compound；Be mainly used in wall material solution preparation material have protein-based, natural plant gum class,
Inorganic salts, polylysine, chitosan, PDDA, cellulose sodium sulfate, polymethylene bisguanides etc.,
The present invention prepares wall material solution using chitosan；
F4：Submerged fermentation culture medium is added in fermentation tank by the 60 ~ 80% of fermenter volume, after stirring, by potting of fermenting
Sterilized well；After submerged fermentation culture medium cooling, magnetic obtained by F3 is carried into thalline microcapsules and connect by inoculum concentration for 10 ~ 12%
Kind obtains submerged fermentation liquid into submerged fermentation culture medium；It is 5.2 ~ 6.2 to adjust submerged fermentation liquid pH, controls submerged fermentation temperature
For 22 ~ 25 DEG C, filtrated air throughput is 2.0 ~ 2.2 m3/ min, 50 ~ 80r/min of mixing speed, tank presses 38 ~ 45Pa, and leads to
Enter high-pressure pulse electric and carry out submerged fermentation, after fermenting 72 ~ 144 hours, discharge submerged fermentation mash；
F5：Fermentation liquid obtained by F4 is taken, filtering adds auxiliary material to carry out pasteurization after allocating, and packing both obtains finished product tremella beverage.
The technical principle that the present invention realizes is：Added with beneficial to thalli growth and be advantageous to intracellular organic matter synthesis it is compound
Amino acid improves the speed of thalli growth and intracellular organic matter synthesis in culture medium；Magnetic field and the effect of high-pressure pulse electric, no
It only can significantly promote thalli growth, moreover it is possible to improve metabolic activity in cells, increase membrane passage；In addition, microcapsules
The thalline of change, influence of the raising to thalline of external substance concentration is reduced, be more beneficial for releasing for thalli growth and intracellular organic matter
Put；In the submerged fermentation system, microencapsulation thalline can under the electric field action of high intensity continuing fermentation；With being entered with free thalline
Row submerged fermentation is compared, and the thalline of microencapsulation can also be utilized repeatedly, be easily isolated, and improves production efficiency.
As a further improvement on the present invention, described electromagnetic field be copper cash around radome formed solenoid, by copper cash with
Rectifier, pressure regulator, power supply are connected to form electromagnetic field generation device, and seed liquor is positioned in radome and cultivated, and pass through pressure regulation
Device changes voltage to change magnetic induction intensity.The magnetic induction intensity of culture seed liquor be 10 ~ 20mT, the magnetic fields time for 5 ~
20min/h。
As a further improvement on the present invention, be defined by 1000ml amounts, seed liquor medium pH be 5.8 ~ 6.2, containing with
The component of lower ratio of weight and number：30 ~ 40 parts of glucose, 15 ~ 25 parts of soluble starch, KH2PO40.8 ~ 1.2 part, MgSO4•7H2O
0.5 ~ 1 part, 0.9 ~ 1.1 part of compound amino acid.The compound amino acid is first addition glutamic acid and glycine 1:1 combination 0.5 ~
0.6 part, add glycine and serine 1:1 0.4 ~ 0.5 part of combination.
As a further improvement on the present invention, described magnetic material is the ferroso-ferric oxide of purity assay grade, in sea
Content is 0.004 ~ 0.006g/ml in solution of sodium alginate.
As a further improvement on the present invention, described wall material solution concentration is 0.8 ~ 1.2mg/ml, by 4 ~ 60,000 molecular weight
Chitosan be dissolved in volume ratio be 1% acetum in be prepared.
As a further improvement on the present invention, the magnetic carries the preparation of thalline microcapsules, using peristaltic pump with it is multiple simultaneously
Row combinations of syringe, forms semi-automatic Dropping feeder, and syringe needle is special orifice aperture syringe needle, and orifice aperture is 420 ~
Between 460 μm, obtained microcapsule diameter is between 300 ~ 450 μm.
As a further improvement on the present invention, the submerged fermentation culture medium contains following components in percentage by weight：Wheat
Bud sugar 3 ~ 5%, soybean protein isolate 3 ~ 5%, pollen powder of chrysanthemun flower 1 ~ 1.8%, corn flour 1 ~ 2%, wolfberry fruit powder 0.2 ~ 0.5%, camellia oil 0.1 ~
0.3%th, magnesium sulfate 0.2 ~ 0.3%, zinc sulfate 0.2 ~ 0.3%, dipotassium hydrogen phosphate 0.2 ~ 0.3%, sodium chloride 0.2 ~ 0.3%, combination amino
Acid 0.5 ~ 0.7%.Described combination amino acid is glutamic acid and glycine 1:1 combination.
As a further improvement on the present invention, the high-pressure pulse electric is arranged in fermentation tank, in fermentation top tank structure phase
Position is made up of two pieces of parallel electrode plates, battery lead plate is parallel with fermentation tank tank skin, and the size of battery lead plate can be according to fermentation tank
Actual volume is adjusted and installed, and battery lead plate height will exceed zymotic fluid liquid level, ensures fermentation liquid energy fully in the electric field, and two
The positive and negative the two poles of the earth of plate electrode respectively with Pulser apparatus outside tank body are connected, and Pulser apparatus connection supply unit forms high pressure
Pulse electric-field generating device；Described high-pressure pulse electric electric field frequency is 40 ~ 60Hz, the μ s of pulsewidth 20 ~ 60, electric-field intensity 20 ~
50v/m, electric field action time are 20 ~ 40min.
As a further improvement on the present invention, described adjustable electromagnetic field is magnetic field caused by electrified wire；Fermenting
Tank wall is provided with major diameter wire, and wire is perpendicular to the ground to be affixed on tank skin, and wire both ends connect the power supply of adjustable voltage；It is deep
Layer fermentation closes power supply when carrying out and does not produce magnetic field, at the end of submerged fermentation, turns on the power, is adjusted by adjusting voltage swing
Magnetic field intensity.Due to Fe3O4The introducing of magnetic particle, by being passed through electromagnetic field to Zymolysis Equipment, magnetic microsphere is carried cell and determine
To movement and it is attached on fermentation tank tank skin, after discharging fermentation liquid, is passed through new submerged fermentation culture medium, cancels electromagnetic field,
In Fe3O4Under superparamagnetism effect, well suspended in fresh medium, carries out secondary fermentation to microballoon again, so as to realize fermentation
During metabolite and immobilized cell online, quick and serialization lock out operation.
The technical program has the following advantages that：
1. compound amino acid improves the synthesis of thalli growth speed and intracellular organic matter, the cycle of continuous submerged fermentation is shortened；
Magnetic fields significantly accelerate thalli growth speed, and high-pressure pulse electric enhances metabolic activity in cells, increases cell membrane penetration
Property, make intracellular organic matter secretion more abundant；The tremella beverage being prepared into using the present invention, tremella polysaccharides content >=8.98g/L, together
When do not influence the taste and flavor of beverage.
2. microencapsulation thalline carries out submerged fermentation, protect somatic cells not crushed in high-pressure pulse electric, reduce
The influence that external substance concentration is secreted to thalli growth and intracellular organic matter, cell density is big, reaction speed is fast after microencapsulation,
Resistance to malicious ability is strong, and the cyst wall of semi-transparent film character is more beneficial for the secretion of intracapsular small-molecule substance；Microencapsulation thalline can enter simultaneously
The multiple batches of fermentation of row, magnetic material is introduced in microcapsules, is easy to be separated with zymotic fluid, improves production efficiency, simultaneously
Nutritional ingredient, thalli growth and the metabolism of zymotic fluid are not influenceed；4 ~ 5 batches can be carried out by the submerged fermentation of the present invention.
4. being added in submerged fermentation culture medium, pollen powder of chrysanthemun flower, wolfberry fruit powder, corn flour, camellia oil etc. are natural, have medicinal valency
The material of value so that tremella beverage mouthfeel more preferably, has medicinal curative effect, improves the nutritive value of tremella beverage.
Embodiment
Technical scheme is further illustrated with reference to embodiment.
Tremella fructification used is that the high-quality nothing of in the market purchase is gone mouldy disease-free Tremella fructification in embodiment.
Fermented in embodiment using 50L fermentation tanks.
Embodiment 1
F1：The present embodiment takes Tremella fructification 50g, cleans dries pulverizing to 100 mesh and obtains white fungus powder, by white fungus powder and water according to material
Liquor ratio 1:40 well mixed sterilizings, you can obtain seed material.
F2：The inoculum concentration of the present embodiment seed liquor by weight percentage 15% is seeded to the compound amino acid culture after sterilizing
In base, it is subsequently placed on shaking table and is passed through electromagnetic field and cultivated, after 23 DEG C, 48 hours of cultivation temperature, obtains seed liquor；
The present embodiment electromagnetic field intensity is 10mT, action time 20min/h；
The present embodiment seed liquid culture medium adds 7.5 parts of soluble starch, 15 parts of glucose, KH in terms of 500ml water2PO40.4
Part, MgSO4•7H20.25 part of O, glutamic acid and glycine 1:1 0.5 part of combination；It is 6.2 to adjust pH, is stirring evenly and then adding into sweet ammonia
Acid and serine 1:1 0.4 part of combination, is sub-packed in 250ml triangular flasks, liquid amount 100ml, 121 DEG C of high-temperature sterilization 30min, treats
Used after being cooled to room temperature.
F3：The sediment that the centrifugation of F2 seed liquors is obtained, is aseptically well mixed with magnetic sodium alginate soln
Obtain mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out solidification 30min, magnetic is obtained and carries thalline alginic acid
Calcium coagulates pearl；Magnetic calcium alginate is coagulated into pearl with water washing is distilled 2 times, then aseptically mixed with the wall material solution after sterilizing
Close, 30min is vibrated under 200rpm rotating speeds and carries out film formation reaction, obtain magnetic and carry thalline microcapsules；
The magnetic sodium alginate soln of the present embodiment, it is by magnetic material Fe3O4It is added in 20mg/ml sodium alginate solns and mixes
Conjunction is uniformly prepared, Fe3O4Content is 0.004g/ml in magnetic Na-alginate solution；
The wall material solution concentration of the present embodiment is 0.8mg/ml, is dissolved in 1% by 40,000 molecular weight chitosans（v/v）Made in acetum
It is standby and obtain；
The calcium chloride solution concentration of the present embodiment is 18mg/ml；
The present embodiment mixed liquor is 1 with calcium chloride solution volume ratio：1.5；
It is 1 that the present embodiment magnetic calcium alginate, which coagulates pearl with wall material solution volume ratio,：5；
F4：Submerged fermentation culture medium is added in fermentation tank by the 60% of fermenter volume, after stirring, fermentation tank sealed
Sterilized；After submerged fermentation culture medium cooling, magnetic obtained by F3 is carried into thalline microcapsules and is seeded to depth by inoculum concentration for 12%
Submerged fermentation liquid is obtained in layer fermentation medium；It is 6.2 to adjust submerged fermentation liquid pH, and it is 25 DEG C to control submerged fermentation temperature, nothing
Bacterium air vent amount is 2.2 m3/ min, mixing speed 50r/min, tank pressure 38Pa, and be passed through high-pressure pulse electric and carry out deep layer
Fermentation, after fermenting 72 hours, adjustable electromagnetic field outside fermentation tank is opened, magnetic field intensity 50mT, action time, 20min carried magnetic
The outer capsule of thalline is attracted to fermentor walls, is then discharged out fermentation liquid, then accesses new culture medium, cancels electromagnetic field, regulation hair
Ferment parameter is consistent with aforementioned parameters, carries out submerged fermentation.
The present embodiment submerged fermentation culture medium contains following components in percentage by weight：Maltose 3%, soybean protein isolate
3%th, pollen powder of chrysanthemun flower 1%, corn flour 1%, wolfberry fruit powder 0.2%, camellia oil 0.1%, magnesium sulfate 0.2%, zinc sulfate 0.2%, dipotassium hydrogen phosphate
0.2%th, sodium chloride 0.2%, glutamic acid and glycine 1:1 combination 0.5%；
The present embodiment high-voltage pulse electric field frequencies range is 40Hz, pulsewidth 60 μ s, electric-field intensity 20v/m, and the electric field action time is
40min。
F5：After taking fermentation liquid filtering obtained by F4, after addition auxiliary material is allocated, dispense and obtain into after progress pasteurization
Product.
Wherein, first batch submerged fermentation, fermentation liquid polyoses content are 6.23g/L；Second lot submerged fermentation, fermentation
Mash polyoses content is 7.08g/L；3rd batch submerged fermentation, fermentation liquid polyoses content are 7.84g/L；4th deep layer is sent out
Ferment, fermentation liquid polyoses content are 7.21g/L.
Embodiment 2
F1：The present embodiment takes 10cm2The white fungus mycelia of flat board culture, goes the removal of impurity, with water according to solid-liquid ratio 1:60 is well mixed,
Seed material can be obtained.
F2：The inoculum concentration of the present embodiment seed liquor by weight percentage 20% is seeded to the compound amino acid culture after sterilizing
In base, it is subsequently placed on shaking table and is passed through electromagnetic field and cultivated, after 24 DEG C, 24 hours of cultivation temperature, obtains seed liquor；
The present embodiment electromagnetic field intensity is 20mT, action time 5min/h；
The present embodiment seed liquid culture medium adds 25 parts of soluble starch, 40 parts of glucose, KH in terms of 1000ml water2PO41.2
Part, MgSO4•7H21 part of O, glutamic acid and glycine 1:1 0.6 part of combination；It is 5.8 to adjust pH, is stirring evenly and then adding into glycine
With serine 1:1 0.5 part of combination, is sub-packed in 250ml triangular flasks, liquid amount 100ml, 121 DEG C of high-temperature sterilization 30min, treats cold
But to using after room temperature.
F3：The sediment that the centrifugation of F2 seed liquors is obtained, is aseptically well mixed with magnetic sodium alginate soln
Obtain mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out solidification 20min, magnetic is obtained and carries thalline alginic acid
Calcium coagulates pearl；Magnetic calcium alginate is coagulated into pearl with water washing is distilled 3 times, then aseptically mixed with the wall material solution after sterilizing
Close, 30min is vibrated under 200rpm rotating speeds and carries out film formation reaction, obtain magnetic and carry thalline microcapsules；
The magnetic sodium alginate soln of the present embodiment, it is by magnetic material Fe3O4It is added in 25mg/ml sodium alginate solns and mixes
Conjunction is uniformly prepared, Fe3O4Content is 0.006g/ml in magnetic Na-alginate solution；
The wall material solution concentration of the present embodiment is 1.2mg/ml, is dissolved in 1% by 60,000 molecular weight chitosans（v/v）Made in acetum
It is standby and obtain；
The calcium chloride solution concentration of the present embodiment is 22mg/ml；
The present embodiment mixed liquor is 1 with calcium chloride solution volume ratio：2；
It is 1 that the present embodiment magnetic calcium alginate, which coagulates pearl with wall material solution volume ratio,：10；
F4：Submerged fermentation culture medium is added in fermentation tank by the 80% of fermenter volume, after stirring, fermentation tank sealed
Sterilized；After submerged fermentation culture medium cooling, magnetic obtained by F3 is carried into thalline microcapsules and is seeded to depth by inoculum concentration for 10%
Submerged fermentation liquid is obtained in layer fermentation medium；It is 5.2 to adjust submerged fermentation liquid pH, and it is 22 DEG C to control submerged fermentation temperature, nothing
Bacterium air vent amount is 2.0m3/ min, mixing speed 80r/min, tank pressure 45Pa, and be passed through high-pressure pulse electric and carry out deep layer hair
Ferment, after fermenting 144 hours, adjustable electromagnetic field outside fermentation tank is opened, magnetic field intensity 100mT, action time, 30min carried magnetic
The outer capsule of thalline is attracted to fermentor walls, is then discharged out fermentation liquid, then accesses new culture medium, cancels electromagnetic field, regulation hair
Ferment parameter is consistent with aforementioned parameters, carries out submerged fermentation.
The present embodiment submerged fermentation culture medium contains following components in percentage by weight：Maltose 5%, soybean protein isolate
5%th, pollen powder of chrysanthemun flower 1.8%, corn flour 2%, wolfberry fruit powder 0.5%, camellia oil 0.3%, magnesium sulfate 0.3%, zinc sulfate 0.3%, phosphoric acid hydrogen two
Potassium 0.3%, sodium chloride 0.3%, glutamic acid and glycine 1:1 combination 0.7%；
The present embodiment high-voltage pulse electric field frequencies range is 60Hz, pulsewidth 20 μ s, electric-field intensity 50v/m, and the electric field action time is
40min。
F5：After taking fermentation liquid filtering obtained by F4, after addition auxiliary material is allocated, dispense and obtain into after progress pasteurization
Product.
Wherein, first batch submerged fermentation, fermentation liquid polyoses content are 7.23g/L；Second lot submerged fermentation, fermentation
Mash polyoses content is 7.65g/L；3rd batch submerged fermentation, fermentation liquid polyoses content are 8.46g/L；4th batch deep layer
Fermentation, fermentation liquid polyoses content is 8.41g/L；5th batch submerged fermentation, the old liquid polyoses content of big foot are 8.29g/L.
Embodiment 3
F1：The present embodiment takes 20cm2The white fungus mycelia of flat board culture, goes the removal of impurity, with water according to solid-liquid ratio 1:50 is well mixed,
Seed material can be obtained.
F2：The inoculum concentration of the present embodiment seed liquor by weight percentage 15% is seeded to the compound amino acid culture after sterilizing
In base, it is subsequently placed on shaking table and is passed through electromagnetic field and cultivated, after 25 DEG C, 36 hours of cultivation temperature, obtains seed liquor；
The present embodiment electromagnetic field intensity is 15mT, action time 15min/h；
The present embodiment seed liquid culture medium adds 15 parts of soluble starch, 30 parts of glucose, KH in terms of 1000ml water2PO41 part,
MgSO4•7H20.8 part of O, glutamic acid and glycine 1:1 0.6 part of combination；Adjust pH be 6.0, be stirring evenly and then adding into glycine with
Serine 1:1 0.4 part of combination, is sub-packed in 250ml triangular flasks, liquid amount 100ml, 121 DEG C of high-temperature sterilization 30min, to be cooled
Used after to room temperature.
F3：The sediment that the centrifugation of F2 seed liquors is obtained, is aseptically well mixed with magnetic sodium alginate soln
Obtain mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out solidification 30min, magnetic is obtained and carries thalline alginic acid
Calcium coagulates pearl；Magnetic calcium alginate is coagulated into pearl with water washing is distilled 3 times, then aseptically mixed with the wall material solution after sterilizing
Close, 30min is vibrated under 200rpm rotating speeds and carries out film formation reaction, obtain magnetic and carry thalline microcapsules；
The magnetic sodium alginate soln of the present embodiment, it is by magnetic material Fe3O4It is added in 22mg/ml sodium alginate solns and mixes
Conjunction is uniformly prepared, Fe3O4Content is 0.005g/ml in magnetic Na-alginate solution；
The wall material solution concentration of the present embodiment is 1.0mg/ml, is dissolved in 1% by 50,000 molecular weight chitosans（v/v）Made in acetum
It is standby and obtain；
The calcium chloride solution concentration of the present embodiment is 20mg/ml；
The present embodiment mixed liquor is 1 with calcium chloride solution volume ratio：2；
It is 1 that the present embodiment magnetic calcium alginate, which coagulates pearl with wall material solution volume ratio,：8；
F4：Submerged fermentation culture medium is added in fermentation tank by the 80% of fermenter volume, after stirring, fermentation tank sealed
Sterilized；After submerged fermentation culture medium cooling, magnetic obtained by F3 is carried into thalline microcapsules and is seeded to depth by inoculum concentration for 11%
Submerged fermentation liquid is obtained in layer fermentation medium；It is 6 to adjust submerged fermentation liquid pH, and it is 25 DEG C to control submerged fermentation temperature, sterile
Air vent amount is 2.2m3/ min, mixing speed 80r/min, tank pressure 40Pa, and be passed through high-pressure pulse electric and carry out deep layer hair
Ferment, after fermenting 120 hours, adjustable electromagnetic field outside fermentation tank is opened, magnetic field intensity 100mT, action time, 30min carried magnetic
The outer capsule of thalline is attracted to fermentor walls, is then discharged out fermentation liquid, then accesses new culture medium, cancels electromagnetic field, regulation hair
Ferment parameter is consistent with aforementioned parameters, carries out submerged fermentation.
The present embodiment submerged fermentation culture medium contains following components in percentage by weight：Maltose 4%, soybean protein isolate
4%th, pollen powder of chrysanthemun flower 1.5%, corn flour 1.5%, wolfberry fruit powder 0.4%, camellia oil 0.2%, magnesium sulfate 0.2%, zinc sulfate 0.2%, phosphoric acid hydrogen
Dipotassium 0.2%, sodium chloride 0.2%, glutamic acid and glycine 1:1 combination 0.6%；
The present embodiment high-voltage pulse electric field frequencies range is 50Hz, pulsewidth 15 μ s, electric-field intensity 35v/m, and the electric field action time is
30min。
F5：After taking fermentation liquid filtering obtained by F4, after addition auxiliary material is allocated, dispense and obtain into after progress pasteurization
Product.
Wherein, first batch submerged fermentation, fermentation liquid polyoses content are 7.81g/L；Second lot submerged fermentation, fermentation
Mash polyoses content is 8.55g/L；3rd batch submerged fermentation, fermentation liquid polyoses content are 8.98g/L；4th deep layer is sent out
Ferment, fermentation liquid polyoses content are 8.50g/L；5th batch submerged fermentation, polyoses content is 8.01g/L in fermentation liquid.
Comparative example 4
F1：The present embodiment takes Tremella fructification 50g, cleans dries pulverizing to 100 mesh and obtains white fungus powder, by white fungus powder and water according to material
Liquor ratio 1:40 well mixed sterilizings, you can obtain seed material.
F2：The inoculum concentration of the present embodiment seed liquor by weight percentage 15% is seeded to the compound amino acid culture after sterilizing
In base, it is subsequently placed on shaking table and is cultivated, after 25 DEG C, 72 hours of cultivation temperature, obtains seed liquor；
The present embodiment seed liquid culture medium adds 30 parts of soluble starch, 20 parts of glucose, KH in terms of 1000ml water2PO41 part,
MgSO4•7H20.8 part of O, glutamic acid and glycine 1:16 parts of combination；It is 6.0 to adjust pH, is stirring evenly and then adding into glycine and silk
Propylhomoserin 1:14 parts of combination, is sub-packed in 250ml triangular flasks, liquid amount 100ml, 121 DEG C of high-temperature sterilization 30min, is cooled to room
Used after temperature.
F3：Submerged fermentation culture medium is added in fermentation tank by the 80% of fermenter volume, after stirring, by fermentation tank
Seal and sterilized；After submerged fermentation culture medium cooling, seed liquor obtained by F2 is seeded to submerged fermentation by inoculum concentration for 11%
Submerged fermentation liquid is obtained in culture medium；It is 6 to adjust submerged fermentation liquid pH, and it is 25 DEG C to control submerged fermentation temperature, and filtrated air leads to
Tolerance is 2.2m3/ min, mixing speed 80r/min, tank pressure 40Pa, after fermenting 190 hours, discharge fermentation liquid.
The present embodiment submerged fermentation culture medium contains following components in percentage by weight：Maltose 4%, soybean protein isolate
4%th, pollen powder of chrysanthemun flower 1.5%, corn flour 1.5%, wolfberry fruit powder 0.4%, camellia oil 0.2%, magnesium sulfate 0.2%, zinc sulfate 0.2%, phosphoric acid hydrogen
Dipotassium 0.2%, sodium chloride 0.2%, glutamic acid and glycine 1:1 combination 0.6%；
In the present embodiment, polyoses content is 5.35g/L in karusen.
F4：After taking fermentation liquid filtering obtained by F3, after addition auxiliary material is allocated, dispense and obtain into after progress pasteurization
Product.
Relevant parameter such as table 1 is determined by above example：
The parameters situation table of table 1
The present invention has the features such as cycle is short, and active constituent content is high in tremella beverage production, and final obtained beverage is without right
Human harmful substance, taste flavor are up to state standards.
Claims (8)
1. a kind of method that continuous submerged fermentation prepares tremella beverage, it is characterised in that preparation method step includes：
F1：Take white fungus mycelia or fructification, after cleaning, drying to moisture are 6 ~ 8%, be crushed to the acquisition of 100 ~ 120 mesh
White fungus powder, white fungus powder and water are pressed into solid-liquid ratio 1：（40~60）It is well mixed, seed material is obtained after sterilizing；
F2：Seed material obtained by F1 is aseptically seeded to the seed liquid culture medium after sterilizing by 15 ~ 20% inoculum concentration
In, it is subsequently placed on shaking table and is passed through electromagnetic field and carry out culture 24 ~ 48 hours, 23 ~ 25 DEG C of cultivation temperature, obtains seed liquor；
F3：The sediment that seed liquor centrifugation obtained by F2 is obtained, is aseptically well mixed with magnetic sodium alginate soln
Obtain mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out 20 ~ 30min of solidification, magnetic is obtained and carries thalline sea
Calcium alginate coagulates pearl；Magnetic calcium alginate is coagulated into pearl with water washing is distilled 2 ~ 3 times, then with the wall material solution after sterilizing in sterile bar
Mixed under part, 15 ~ 30min is vibrated under 200 ~ 230rpm rotating speeds and carries out film formation reaction, obtained magnetic and carry thalline microcapsules；
Described magnetic sodium alginate soln, it is that magnetic material is added in 20 ~ 25mg/ml sodium alginate solns to be well mixed
It is prepared；The wall material solution concentration is 0.8 ~ 1.2mg/ml；Described calcium chloride solution concentration is 18 ~ 22mg/ml；It is described
Mixed liquor is 1 with calcium chloride solution volume ratio：（1.5~2）；It is 1 that the magnetic calcium alginate, which coagulates pearl with wall material solution volume ratio,：
（5~10）；
F4：Submerged fermentation culture medium is added in fermentation tank by the 60 ~ 80% of fermenter volume, after stirring, by potting of fermenting
Sterilized well；After submerged fermentation culture medium cooling, magnetic obtained by F3 is carried into thalline microcapsules and connect by inoculum concentration for 10 ~ 12%
Kind obtains submerged fermentation liquid into submerged fermentation culture medium；It is 5.2 ~ 6.2 to adjust submerged fermentation liquid pH, controls submerged fermentation temperature
For 22 ~ 25 DEG C, filtrated air throughput is 2.0 ~ 2.2 m3/ min, 50 ~ 80r/min of mixing speed, tank presses 38 ~ 45Pa, and leads to
Enter high-pressure pulse electric and carry out submerged fermentation, after fermenting 72 ~ 144 hours, discharge submerged fermentation mash；
The submerged fermentation culture medium contains following components in percentage by weight：Maltose 3 ~ 5%, soybean protein isolate 3 ~ 5%, chrysanthemum
Flower pollen 1 ~ 1.8%, corn flour 1 ~ 2%, wolfberry fruit powder 0.2 ~ 0.5%, camellia oil 0.1 ~ 0.3%, magnesium sulfate 0.2 ~ 0.3%, zinc sulfate
0.2 ~ 0.3%, dipotassium hydrogen phosphate 0.2 ~ 0.3%, sodium chloride 0.2 ~ 0.3%, combination amino acid 0.5 ~ 0.7%；
F5：Fermentation liquid obtained by F4 is taken, filtering adds auxiliary material to carry out pasteurization after allocating, and packing both obtains finished product tremella beverage.
2. the method that a kind of continuous submerged fermentation according to claim 1 prepares tremella beverage, it is characterised in that：Described in F1
Electromagnetic field intensity is 10 ~ 20mT, 5 ~ 20min/h of action time.
3. the method that a kind of continuous submerged fermentation according to claim 1 prepares tremella beverage, it is characterised in that：Described in F2
Seed liquor medium pH is 5.8 ~ 6.2, the component containing following ratio of weight and number：30 ~ 40 parts of glucose, soluble starch 15 ~
25 parts, KH2PO40.8 ~ 1.2 part, MgSO4•7H20.5 ~ 1 part of O, 0.9 ~ 1.1 part of compound amino acid.
4. the method that a kind of continuous submerged fermentation according to claim 1 prepares tremella beverage, it is characterised in that：Described in F3
Magnetic material be purity assay grade ferroso-ferric oxide, content is 0.004 ~ 0.006g/ in magnetic sodium alginate soln
ml；The wall material solution preparation method is as follows：The chitosan of molecular weight 4 ~ 60,000 is taken to be dissolved in volume ratio in 1% acetum；Institute
It is 300 ~ 450 μm that the magnetic stated, which carries thalline microcapsule diameter,.
5. the method that a kind of continuous submerged fermentation according to claim 1 prepares tremella beverage, it is characterised in that：Described in F4
Combination amino acid be glutamic acid and glycine 1:1 combination.
6. the method that a kind of continuous submerged fermentation according to claim 1 prepares tremella beverage, it is characterised in that：Described in F4
High-pressure pulse electric be arranged in fermentation tank；High-voltage pulse electric field frequencies range is 40 ~ 60Hz, the μ s of pulsewidth 20 ~ 60, electric-field intensity
20 ~ 50v/m, electric field action time are 20 ~ 40min.
7. the method that a kind of continuous submerged fermentation according to claim 1 prepares tremella beverage, it is characterised in that：F4 is discharged
The method of fermentation liquid is that magnetic is carried into thalline microcapsules orientation using adjustable electromagnetic field to be attracted on fermentation tank tank skin, bacterium solution
After separation, fermentation liquid is discharged；Adjustable electromagnetic field is arranged at fermentation tank wall, 50 ~ 100mT of magnetic field intensity, and action time 20 ~
30min。
8. the method that a kind of continuous submerged fermentation according to claim 3 prepares tremella beverage, it is characterised in that：It is described multiple
Amino acid is closed first to add glutamic acid and glycine 1:1 0.5 ~ 0.6 part of combination, adds glycine and serine 1:1 combination 0.4
~ 0.5 part.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142448A (en) * | 2021-04-26 | 2021-07-23 | 江西水圣实业有限公司 | Medlar and chrysanthemum fermented beverage and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101467780A (en) * | 2007-12-27 | 2009-07-01 | 天津市中英保健食品有限公司 | Edible fungus beverage |
| DE102012210740A1 (en) * | 2011-06-25 | 2012-12-27 | Werner Sitzmann | Accelerating material conversion in bioreactors or fermenters, comprises applying pulsed electrical high voltage fields on material mixture during material conversion |
-
2017
- 2017-09-21 CN CN201710858173.0A patent/CN107594260A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101467780A (en) * | 2007-12-27 | 2009-07-01 | 天津市中英保健食品有限公司 | Edible fungus beverage |
| DE102012210740A1 (en) * | 2011-06-25 | 2012-12-27 | Werner Sitzmann | Accelerating material conversion in bioreactors or fermenters, comprises applying pulsed electrical high voltage fields on material mixture during material conversion |
Non-Patent Citations (3)
| Title |
|---|
| 付璞玉: "直流电场对微生物的影响", 《科技创新与应用》 * |
| 刘萍等: "脉冲电场制备磁性Fe3O4海藻酸盐微球的工艺研究", 《食品与发酵工业》 * |
| 王蓓等: "静磁场辅助液态发酵对樟芝菌丝体及三萜产量的影响", 《中国农业科技导报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142448A (en) * | 2021-04-26 | 2021-07-23 | 江西水圣实业有限公司 | Medlar and chrysanthemum fermented beverage and preparation method thereof |
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