CN107593622A - A kind of preparation method of Se-enriched honey - Google Patents
A kind of preparation method of Se-enriched honey Download PDFInfo
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- CN107593622A CN107593622A CN201711007691.8A CN201711007691A CN107593622A CN 107593622 A CN107593622 A CN 107593622A CN 201711007691 A CN201711007691 A CN 201711007691A CN 107593622 A CN107593622 A CN 107593622A
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Abstract
The invention discloses a kind of preparation method of Se-enriched honey, based on filling spleen feeding, coordinates pollen to feed honeybee by the mode of honeybee free choice feeding, by by analysis for soybean powder, malt flour, Se-enriched yeast powder, saleratus, magnesium sulfate, vitamin C, lysine, pollen substitute Se-enriched feedstuff made of methionine feed honeybee, promote honeybee to assimilate ability, so as to obtain high protein, high nutritive value Se-enriched honey.
Description
Technical field
The present invention relates to honey preparation technology, and in particular to a kind of preparation method of Se-enriched honey.
Background technology
Honey is honeybee takes the nectar or secretion that water content is about 80% from the spending of plant, be stored in oneself second
In stomach, pass through the fermentation of 30 minutes in the presence of invertase in vivo, return in honeycomb and spue, temperature is always maintained in honeycomb
35 DEG C or so, after a period of time, moisture evaporation, turns into the honey that moisture is less than 20%, is stored into nest hole, uses beeswax
Sealing.
Its main component of honey is fructose and glucose, both contents are total account for 70%. still containing a small amount of sucrose (8%),
Maltose, dextrin, natural gum and nitrogen-containing compound, organic acid (about 5%), moisture (16%-25%), volatile oil, pigment, wax,
Natural perfume material, vegetable debris (particularly pollen grain), yeast, enzyme, inorganic salts etc..
The traditional Chinese medical science thinks that honey is sweet, mild-natured, returns spleen, lung, the heart, stomach, large intestine channel.With nourishing Yin and moistening dryness, qi-restoratives moistening lung, solution
Poison, the effect of coordinating the drug actions of a prescription, are usually used in xeropulmonary cough, physically weak, dry constipation of intestines, aphtha, scald, gastral cavity pain, can be with
Solve the rhizome of Chinese monkshood, the poison of monkshood.Contain fructose, glucose, enzyme, protein, vitamin and several mineral materials in honey, often eating can be to prevent
Eliminate-poverty blood, heart disease, enterogastritis etc., and body immunity can be improved.Honey is the natural nutrient food being widely recognized.Experiment
Show, the various inorganic salts similar to human serum concentration are contained in honey, such as iron, calcium, copper, manganese, potassium, phosphorus are also a variety of
Vitamin and organic acid, therefore honey is referred to as " king of serum ".
It is mainly at present quality problems for honey, wherein further relating to indivedual adulterated problems, this problem has perplexed everybody
Decades, still it can't resolve at present.With regard to this, the developing direction honey of honey production is determined, is a kind of the natural of animal sources
Product.Why different with the product such as white sugar, fructose syrup it is, in that its pure natural property.Honey is a primary agriculture
Product, its production process are completed by honeybee.Honey quality is improved, is to need honeybee and bee keeper's joint efforts ability
It is enough to realize.
The content of the invention
In order to solve the above problems, the invention provides a kind of preparation method of Se-enriched honey, is fed with and spends using spleen is filled
Powder freely gathers the mode combined and feeds honeybee, to obtain quality more preferably honey.
The present invention can be achieved through the following technical solutions:
A kind of preparation method of Se-enriched honey, comprises the following steps:
Apis cerana is fed using Se-enriched feedstuff, every group of honeybees feed weekly Se-enriched feedstuff 500-600g, point 2 feedings,
Itself is stored in after bee gathering in vivo, honey, as Se-enriched honey are secreted out of through conversion in the body enzyme fermentation.
The Se-enriched feedstuff includes following component:Analysis for soybean powder 50-60%, malt flour 20-25%, Se-enriched yeast powder 15-
20%.
Also include saleratus 2-2.5mg/kg, magnesium sulfate 0.5-1mg/kg, vitamin C 0.5- in the Se-enriched feedstuff
1mg/kg, lysine 15-20mg/kg, methionine 5-10mg/kg.
The analysis for soybean powder is to select soya bean fresh then, and soya bean is cleaned and dried, is put into mixer and is broken into powder, mistake
Sieve, thick particle place into mixer and stirred to sieving, operate 2-3 times repeatedly, then bean powder is put into small fire in pot and fried to bean powder
Become yellow, when beans perfume (or spice) overflows, produce analysis for soybean powder.
The malt flour is to eluriate wheat fresh then to remove impurity, soaks 24h with clean water, is fully dragged for after expansion
Go out, the vernalization 24-36h at 30 DEG C, when malt length is to 1cm, malt is dried and pulverizes into powder and produces malt flour.
The preparation method of the Se-enriched yeast powder is:
(1) from brewer's yeast as strain;
(2) glucose 50g/L, potassium dihydrogen phosphate 2.5g/L, yeast extract 0.5g/L, ferrous sulfate 0.1g/L, sulfuric acid are weighed
Magnesium 1g/L is well mixed, and regulation pH value is 5-5.5, and sterilize 20min under 107.9kPa pressure, obtains nutrient solution;
(3) malt extract medium is prepared with nutrient solution and brewer's wort, adds the sodium selenite solution of sterilizing in the medium,
Selenium concentration 22-25ug/ml in culture medium;
(4) 5-10% strains amount is accessed in culture medium, shaking speed 200r/min, the fermented and cultured 30- at 28 DEG C
35h, after the emulsion that will ferment centrifuges, through washing, finished product selenium yeast is obtained after drying, finished product Selenium in Selenium-Yeast content is 630-
650mg/kg。
Described feed is used based on filling spleen feeding, coordinates pollen to feed honeybee by the mode of honeybee free choice feeding.
The filling spleen feeding:By Se-enriched feedstuff:High-quality hydromel=2:1 ratio is sufficiently stirred, after Se-enriched feedstuff is swollen
120 mesh sieves are crossed, are sieved on honeycomb, is shaken in sieve, it is uniformly fallen into lair, after filling lair, gently pressed with hairbrush several
Under, feed is in kind filled another side after having filled one side by Song Bianshi.
The present invention possesses following beneficial effect:
The present invention relates to a kind of preparation method of Se-enriched honey, based on filling spleen feeding, coordinates pollen free by honeybee
The mode of feeding feeds honeybee.The various advantages of two ways are combined, its each shortcoming are overcome, so as to obtain high-quality honeybee
Honey.
The present invention is rich as pollen substitute forage feed honeybee, analysis for soybean powder using analysis for soybean powder, malt flour and Se-enriched yeast powder
Containing protein, vitamin and inorganic salts etc., also contain higher energy, and its is cheap;Addition malt flour can aid in
Absorption of the honeybee to analysis for soybean powder, and malt flour can be Starch Conversion into maltose and glucose, it is easier to digested and inhale by honeybee
Receive, moreover it is possible to stimulate honeybee feeding;Se-enriched yeast powder can with replenishing vitamins and main nutrient etc., rich in vitamin, mineral matter,
Amino acid and growth factor, Se-enriched yeast powder of the invention belong to high protein using brewer's yeast as strain, brewer's yeast
Matter source, it is comparatively ideal honeybee feed containing abundant protein.
The present invention is played a part of mutually facilitating, mutually supplied, promoted from the pollen substitute Se-enriched feedstuff for feeding honeybee
Honeybee assimilates ability, and obtains Se-enriched honey by honeybee conversion in the body enzyme fermentation, makes the honey of production rich in height
Albumen, nutritive value are high.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment 1
A kind of preparation method of Se-enriched honey of the present embodiment, apis cerana is fed using Se-enriched feedstuff, every group of honeybees are weekly
Se-enriched feedstuff 550g is fed, point 2 feedings, after making bee gathering, honey is secreted out of through conversion in the body enzyme fermentation, produces selenium-rich honeybee
Honey.
A kind of Se-enriched honey of the present embodiment and preparation method thereof, the Se-enriched feedstuff mainly include following component:Analysis for soybean powder
55%, malt flour 22.5%, Se-enriched yeast powder 17.5%.
Also include saleratus 2.25mg/kg, magnesium sulfate 0.75mg/kg, vitamin C 0.75mg/ in above-mentioned Se-enriched feedstuff
Kg, lysine 17.5mg/kg, methionine 7.5mg/kg.
Above-mentioned analysis for soybean powder is to select soya bean fresh then, and soya bean is cleaned and dried, is put into mixer and is broken into powder, mistake
Sieve, thick particle place into mixer and stirred to sieving, operate 2 times repeatedly, then bean powder is put into small fire in pot and fried to bean powder and is become
Yellow, when beans perfume (or spice) overflows, produce analysis for soybean powder.
Above-mentioned malt flour is to eluriate wheat fresh then to remove impurity, soaks 24h with clean water, is fully dragged for after expansion
Go out, the vernalization 30h at 30 DEG C, when malt length is to 1cm, malt is dried and pulverizes into powder and produces malt flour.
The preparation method of selenium-rich Saccharomyces cerevisiae powder is:
(1) from brewer's yeast as strain;
(2) glucose 50g/L, potassium dihydrogen phosphate 2.5g/L, yeast extract 0.5g/L, ferrous sulfate 0.1g/L, sulfuric acid are weighed
Magnesium 1g/L is well mixed, and regulation pH value is 5.5, and sterilize 20min under 107.9kPa pressure, obtains nutrient solution;
(3) malt extract medium is prepared with nutrient solution and brewer's wort, adds the sodium selenite solution of sterilizing in the medium,
The μ g/mL of selenium concentration 23.5 in culture medium;
(4) 7.5% strain amount is accessed in culture medium, shaking speed 200r/min, the fermented and cultured 32.5h at 28 DEG C,
Will fermentation emulsion centrifuge after, through washing, dry after finished product selenium yeast, finished product Selenium in Selenium-Yeast content is 640mg/kg.
A kind of preparation method of Se-enriched honey of the present embodiment, based on filling spleen feeding, pollen is coordinated freely to be adopted by honeybee
The mode of food feeds honeybee.
Above-mentioned filling spleen feeding:By Se-enriched feedstuff:High-quality hydromel=2:1 ratio is sufficiently stirred, after Se-enriched feedstuff is swollen
120 mesh sieves are crossed, are sieved on honeycomb, is shaken in sieve, it is uniformly fallen into lair, after filling lair, gently pressed with hairbrush several
Under, feed is in kind filled another side after having filled one side by Song Bianshi.
Embodiment 2
A kind of preparation method of Se-enriched honey of the present embodiment, apis cerana is fed using Se-enriched feedstuff, every group of honeybees are weekly
Se-enriched feedstuff 500g is fed, point 2 feedings, after making bee gathering, honey is secreted out of through conversion in the body enzyme fermentation, produces selenium-rich honeybee
Honey.
A kind of Se-enriched honey of the present embodiment and preparation method thereof, the Se-enriched feedstuff mainly include following component:Analysis for soybean powder
50%, malt flour 20%, Se-enriched yeast powder 15%.
In above-mentioned Se-enriched feedstuff also include saleratus 2mg/kg, magnesium sulfate 0.5mg/kg, vitamin C 0.5mg/kg, rely
Propylhomoserin 15mg/kg, methionine 5mg/kg.
Above-mentioned analysis for soybean powder is to select soya bean fresh then, and soya bean is cleaned and dried, is put into mixer and is broken into powder, mistake
Sieve, thick particle place into mixer and stirred to sieving, operate 2 times repeatedly, then bean powder is put into small fire in pot and fried to bean powder and is become
Yellow, when beans perfume (or spice) overflows, produce analysis for soybean powder.
Above-mentioned malt flour is to eluriate wheat fresh then to remove impurity, soaks 24h with clean water, is fully dragged for after expansion
Go out, the vernalization 24h at 30 DEG C, when malt length is to 1cm, malt is dried and pulverizes into powder and produces malt flour.
The preparation method of selenium-rich Saccharomyces cerevisiae powder is:
(1) from brewer's yeast as strain;
(2) glucose 50g/L, potassium dihydrogen phosphate 2.5g/L, yeast extract 0.5g/L, ferrous sulfate 0.1g/L, sulfuric acid are weighed
Magnesium 1g/L is well mixed, and regulation pH value is 5, and sterilize 20min under 107.9kPa pressure, obtains nutrient solution;
(3) malt extract medium is prepared with nutrient solution and brewer's wort, adds the sodium selenite solution of sterilizing in the medium,
The μ g/mL of selenium concentration 22 in culture medium;
(4) 5% strain amount is accessed in culture medium, shaking speed 200r/min, the fermented and cultured 30h at 28 DEG C, will sent out
After kefir milk liquid centrifuges, through washing, finished product selenium yeast is obtained after drying, finished product Selenium in Selenium-Yeast content is 630mg/kg.
A kind of preparation method of Se-enriched honey of the present embodiment, based on filling spleen feeding, pollen is coordinated freely to be adopted by honeybee
The mode of food feeds honeybee.
Above-mentioned filling spleen feeding:By Se-enriched feedstuff:High-quality hydromel=2:1 ratio is sufficiently stirred, after Se-enriched feedstuff is swollen
120 mesh sieves are crossed, are sieved on honeycomb, is shaken in sieve, it is uniformly fallen into lair, after filling lair, gently pressed with hairbrush several
Under, feed is in kind filled another side after having filled one side by Song Bianshi.
Embodiment 3
A kind of preparation method of Se-enriched honey of the present embodiment, apis cerana is fed using Se-enriched feedstuff, every group of honeybees are weekly
Se-enriched feedstuff 600g is fed, point 2 feedings, after making bee gathering, honey is secreted out of through conversion in the body enzyme fermentation, produces selenium-rich honeybee
Honey.
A kind of Se-enriched honey of the present embodiment and preparation method thereof, the Se-enriched feedstuff mainly include following component:Analysis for soybean powder
60%, malt flour 25%, Se-enriched yeast powder 20%.
Also include saleratus 2.5mg/kg, magnesium sulfate 1mg/kg, vitamin C 1mg/kg, bad ammonia in above-mentioned Se-enriched feedstuff
Sour 20mg/kg, methionine 10mg/kg.
Above-mentioned analysis for soybean powder is to select soya bean fresh then, and soya bean is cleaned and dried, is put into mixer and is broken into powder, mistake
Sieve, thick particle place into mixer and stirred to sieving, operate 3 times repeatedly, then bean powder is put into small fire in pot and fried to bean powder and is become
Yellow, when beans perfume (or spice) overflows, produce analysis for soybean powder.
Above-mentioned malt flour is to eluriate wheat fresh then to remove impurity, soaks 24h with clean water, is fully dragged for after expansion
Go out, the vernalization 36h at 30 DEG C, when malt length is to 1cm, malt is dried and pulverizes into powder and produces malt flour.
The preparation method of selenium-rich Saccharomyces cerevisiae powder is:
(1) from brewer's yeast as strain;
(2) glucose 50g/L, potassium dihydrogen phosphate 2.5g/L, yeast extract 0.5g/L, ferrous sulfate 0.1g/L, sulfuric acid are weighed
Magnesium 1g/L is well mixed, and regulation pH value is 5.5, and sterilize 20min under 107.9kPa pressure, obtains nutrient solution;
(3) malt extract medium is prepared with nutrient solution and brewer's wort, adds the sodium selenite solution of sterilizing in the medium,
The μ g/mL of selenium concentration 25 in culture medium;
(4) 10% strain amount is accessed in culture medium, shaking speed 200r/min, the fermented and cultured 35h at 28 DEG C, will sent out
After kefir milk liquid centrifuges, through washing, finished product selenium yeast is obtained after drying, finished product Selenium in Selenium-Yeast content is 650mg/kg.
A kind of preparation method of Se-enriched honey of the present embodiment, based on filling spleen feeding, pollen is coordinated freely to be adopted by honeybee
The mode of food feeds honeybee.
Above-mentioned filling spleen feeding:By Se-enriched feedstuff:High-quality hydromel=2:1 ratio is sufficiently stirred, after Se-enriched feedstuff is swollen
120 mesh sieves are crossed, are sieved on honeycomb, is shaken in sieve, it is uniformly fallen into lair, after filling lair, gently pressed with hairbrush several
Under, feed is in kind filled another side after having filled one side by Song Bianshi.
Comparative example 1
Difference with embodiment 1 is, malt flour is not included in Se-enriched feedstuff.
Comparative example 2
Difference with embodiment 1 is that the mode for feeding honeybee is only to fill spleen feeding, by honeybee free choice feeding, is not had
Coordinate pollen.
Comparative example 3
Difference with embodiment 1 is that the method used is according to Chinese patent《A kind of Se-enriched honey and its preparation side
Method》(number of patent application:CN201510933524.0).
Compliance test result
Choose to pluck the Apis mellifera of the same race based on honey of lungan flowers, place preferably selects the nearly hillside in south orientation, sunny, the back of the body
There is high mountain for screen, above have nature shade, summer leads to south wind, winter resistance north wind, and soil property is fertile, and longan grows vigorous, the florescence
Long, water is sufficient, and traffic is relatively convenient.Method according to above-described embodiment 1~3 and comparative example 1~3 is fed to honeybee, system
Standby honey out carries out unified checking again, as a result as shown in the table:
As seen from the above table, the result that embodiment 1~3 is drawn has no too big difference, illustrates the model allowed in some dosages
Se-enriched honey can be prepared in enclosing, and it is common while ensureing that selenium content increases significantly also to effectively improve honey
The index of quality, such as improve honey output, glucose content, fructose content and protein content;Comparative example 1 and embodiment 1
Compare, differ too big in terms of yield, be easy to because comparative example does not have to add by the malt flour of honeybee digestion, malt flour can be
Starch Conversion stimulates honeybee feeding, so as to substantial amounts of Se-enriched yeast powder of eating food, supplements more nutrition into maltose and glucose
Material;Comparative example 2 is compared with embodiment 1, and difference is larger in terms of the protein of honey, because pollen energy auxiliary fodder promotes honey
Honeybee assimilates ability, and Se-enriched honey is obtained by honeybee conversion in the body enzyme fermentation, the honey of production is rich in high protein,
Nutritive value is high;Comparative example 3 is compared with embodiment 1, and index all shows deficiency in every respect, because lacking malt flour in comparative example
The composition of honeybee food Se-enriched feedstuff can be stimulated, then lacks this dispensing rich in nutrition of analysis for soybean powder, pollen, the sugarcane in feed
The glucose that sugar is less than in embodiment is easier to absorb.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.
Claims (8)
1. a kind of preparation method of Se-enriched honey, it is characterised in that feed apis cerana using Se-enriched feedstuff, every group of honeybees are weekly
Se-enriched feedstuff 500-600g is fed, divides 2 feedings, itself is stored in after bee gathering in vivo, is secreted out of through conversion in the body enzyme fermentation
Honey, as Se-enriched honey.
2. a kind of Se-enriched honey according to claim 1 and preparation method thereof, it is characterised in that the Se-enriched feedstuff includes
Following component:Analysis for soybean powder 50-60%, malt flour 20-25%, Se-enriched yeast powder 15-20%.
3. the preparation method of a kind of Se-enriched honey according to claim 2, it is characterised in that also wrapped in the Se-enriched feedstuff
Include saleratus 2-2.5mg/kg, magnesium sulfate 0.5-1mg/kg, vitamin C 0.5-1mg/kg, lysine 15-20mg/kg, egg ammonia
Sour 5-10mg/kg.
4. the preparation method of a kind of Se-enriched honey according to claim 2, it is characterised in that the analysis for soybean powder is to select to work as
Year fresh soya bean, soya bean is cleaned and dried, is put into mixer and is broken into powder, sieve, thick particle places into mixer stirring
To sieving, operate 2-3 times repeatedly, then bean powder is put into small fire in pot and fried to bean powder and becomes yellow, when beans perfume (or spice) overflows, produce soya bean
Powder.
5. the preparation method of a kind of Se-enriched honey according to claim 2, it is characterised in that the malt flour is by then
Fresh wheat, which is eluriated, removes impurity, soaks 24h with clean water, is fully pulled out after expansion, vernalization 24-36h, malt at 30 DEG C
It is long to 1cm when, malt is dried and pulverizes into powder and produces malt flour.
A kind of 6. preparation method of Se-enriched honey according to claim 2, it is characterised in that the system of the Se-enriched yeast powder
Preparation Method is:
(1) from brewer's yeast as strain;
(2) glucose 50g/L, potassium dihydrogen phosphate 2.5g/L, yeast extract 0.5g/L, ferrous sulfate 0.1g/L, magnesium sulfate 1g/ are weighed
L is well mixed, and regulation pH value is 5-5.5, and sterilize 20min under 107.9kPa pressure, obtains nutrient solution;
(3) malt extract medium is prepared with nutrient solution and brewer's wort, adds the sodium selenite solution of sterilizing in the medium, cultivated
Selenium concentration 22-25ug/ml in base;
(4) 5-10% strains amount is accessed in culture medium, shaking speed 200r/min, the fermented and cultured 30-35h at 28 DEG C, will
After emulsion of fermenting centrifuges, through washing, finished product selenium yeast is obtained after drying, finished product Selenium in Selenium-Yeast content is 630-650mg/
kg。
A kind of 7. preparation method of Se-enriched honey according to claim 1, it is characterised in that based on filling spleen feeding,
Pollen is coordinated to feed honeybee by the mode of honeybee free choice feeding.
A kind of 8. preparation method of Se-enriched honey according to claim 7, it is characterised in that the filling spleen feeding:By richness
Selenium feed:High-quality hydromel=2:1 ratio is sufficiently stirred, and is crossed 120 mesh sieves after Se-enriched feedstuff is swollen, is sieved on honeycomb, side sieve
Side is shaken, and it is uniformly fallen into lair, after filling lair, gently pressed with hairbrush it is several under, make feed by Song Bianshi, fill good one side
Another side is in kind filled afterwards.
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CN112189759A (en) * | 2020-10-30 | 2021-01-08 | 中国农业科学院蜜蜂研究所 | Bee feed and preparation method and application thereof |
CN114403405A (en) * | 2021-12-30 | 2022-04-29 | 安徽盛晨食品有限公司 | Selenium-rich honey composition, honey raw material and preparation method of selenium-rich honey |
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Application publication date: 20180119 |