CN107561012A - It is divided detection means - Google Patents
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- CN107561012A CN107561012A CN201710981298.2A CN201710981298A CN107561012A CN 107561012 A CN107561012 A CN 107561012A CN 201710981298 A CN201710981298 A CN 201710981298A CN 107561012 A CN107561012 A CN 107561012A
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Abstract
The invention discloses one kind to be divided detection means, is made up of girdle prism, electric-controlled switch door, spectroscope, dichroic mirror, the first optical filter, collimation lens, LED light source, the second optical filter, the second condenser lens, aperture and photodetectors such as the first condenser lens, the first total reflective mirror, the second total reflective mirror, the 3rd total reflective mirror, right angles.Apparatus of the present invention can be realized to be excited respectively to adjacent several millimeters of fluorometric reagent line arranged side by side, and collect the respective fluorescence intensity of detection, interfering for fluorescent test paper T lines and C line fluorescence can be excluded completely, and the device has the characteristics that compact-sized, small volume, stability are good.
Description
Technical field
The present invention relates to a kind of fluorescence detection device of hand-held immunity analysis instrument, more particularly to a kind of closely phosphor strip
The light splitting detection means of line detection.
Background technology
The research of fluorescence immunoassay instrument device can realize the quantification detection to test strips using own warp, but at present
Most instruments obtain C lines and T line optical signal intensity using the method for optical mechaical scanning, and equipment instrument is excessive, is not easy to
Carrying and Site Detection, constrain the application and popularization of this technology.
Existing fluorescence immunoassay instrument device volume-diminished improves with quantitative accuracy of detection has implacable lance
Shield.
The content of the invention
Goal of the invention：For problem above, the present invention proposes a kind of light splitting detection means, by C lines and T in optical design
Linear light signal physically separates.
Technical scheme：To realize the purpose of the present invention, the technical solution adopted in the present invention is：One kind light splitting detection dress
Put, including the girdle prism such as the first condenser lens, the first total reflective mirror, the second total reflective mirror, the 3rd total reflective mirror, right angle, electric-controlled switch door,
Spectroscope, dichroic mirror, the first optical filter, the second condenser lens, aperture, photodetector, the second optical filter, collimation lens
And LED light source；Wherein, the girdle prism such as right angle is located above the first condenser lens, and the axis of girdle prism such as right angle and first focuses on
The middle overlapping of axles of lens；First total reflective mirror, the 3rd total reflective mirror are located at the girdle prism both sides such as right angle, and respectively with the girdle prism such as right angle
Two right-angle surfaces are parallel；Using the axis of the first condenser lens as optical axis, the second total reflective mirror and spectroscope and optical axis are at 45 °, and second is complete
Anti- mirror is located at directly over the first total reflective mirror, and spectroscope is located at directly over the 3rd total reflective mirror, and electric-controlled switch door is located at the second total reflective mirror
Between spectroscope；Dichroic mirror is at 45 ° with optical axis, the first optical filter, the second condenser lens, aperture and light on receiving light path
Electric explorer is arranged in order；The second optical filter, collimation lens and LED light source are arranged in order on excitation light path.
When carrying out fluoroscopic examination to C lines, the exciting light that LED light source is sent after collimation lens and the second optical filter by transmiting
To dichroic mirror, reflex on spectroscope through dichroic mirror, be divided through spectroscope；Exciting light is reflected for 90 ° by reflection through total reflective mirror all the way
Onto the right angle reflecting surface of the girdle prisms such as right angle, pass through the first condenser lens after the right angle reflective surface of the girdle prisms such as right angle
The mirror body optically focused of side projects the C lines direction of fluorescence immunoassay test paper；Another way exciting light is opened transmitted through spectroscope due to automatically controlled
Close the door and close and the T lines of test paper can not be excited；The fluorescence that C lines are excited to send travels to dichroic mirror according to opposite direction, is reflected to
First optical filter of receiving light path, by the second condenser lens after optical filtering, converge to aperture and be received by a photoelectric detector.
When carrying out fluoroscopic examination to T lines, the exciting light that LED light source is sent after collimation lens and the second optical filter by transmiting
Dichroic mirror is crossed, is reflexed to through dichroic mirror on spectroscope, is divided through spectroscope；Exciting light is reflected for 90 ° by reflection through total reflective mirror all the way
Onto the right angle reflecting surface of the girdle prisms such as right angle, pass through the first condenser lens after the right angle reflective surface of the girdle prisms such as right angle
The mirror body optically focused of side projects the C lines direction of fluorescence immunoassay test paper；The fluorescence that C lines are excited to send travels to two according to opposite direction
Look mirror, the first optical filter of receiving light path is reflected to, by the second condenser lens after optical filtering, converges to aperture by photoelectricity
Detector receives；Another way exciting light is transmitted through spectroscope, and the electric-controlled switch door by opening projects the second total reflective mirror, through anti-
Penetrate 90 ° and project the first total reflective mirror, then the right angle reflective surface through girdle prisms such as right angles, pass through the first condenser lens side
Mirror body optically focused projects the T lines direction of fluorescence immunoassay test paper；The fluorescence that T lines are excited to send travels to dichroic mirror according to opposite direction,
The first optical filter of receiving light path is reflected to, by the second condenser lens after optical filtering, converges to aperture by photodetection
Device receives；The fluorescence that photodetector receives is C lines and T line fluorescence intensity sums.
Beneficial effect：Instant invention overcomes existing fluorescence immunoassay instrument device volume-diminished and quantitative accuracy of detection to carry
Contradiction present in height, measurement accuracy are high, are easy to carry and Site Detection, there is provided one kind is in optical design by C lines and T lines
The detection means that optical signal physically separates, it can be used for the research and development of fluorescence immunoassay instrument device.
Apparatus of the present invention can be realized to be excited respectively to adjacent several millimeters of fluorometric reagent line arranged side by side, and collects spy
Respective fluorescence intensity is surveyed, interfering for fluorescent test paper T lines and C line fluorescence can be excluded completely, the device has structure tight
Gather, the features such as small volume, stability are good.
Brief description of the drawings
Fig. 1 is the explosive view of present invention light splitting detection means；
Fig. 2 is the front view of present invention light splitting detection means light path；
Fig. 3 is the top view of present invention light splitting detection means light path.
Embodiment
Technical scheme is further described with reference to the accompanying drawings and examples.
As shown in figure 1, the light splitting detection means of the present invention, is detected applied to closely fluorescence striped, including first focuses on
The girdle prisms 5 such as lens 1, the first total reflective mirror 2, the second total reflective mirror 3, the 3rd total reflective mirror 4, right angle, electric-controlled switch door 6, spectroscope 7,
Dichroic mirror 8, the second optical filter 13, collimation lens 14, LED light source 15, the first optical filter 9, the second condenser lens 10, aperture
11 and photodetector 12.
As shown in Figures 2 and 3, the middle overlapping of axles of the girdle prism 5 such as the axis of the first condenser lens 1 and right angle, ensures with right angle
T lines light path and C line light paths are divided into the left and right sides by the rectangular edge Deng girdle prism 5 for light path line of demarcation, and first is complete
Anti- mirror 2, the 3rd total reflective mirror 4 are parallel with 5 liang of right-angle surfaces of girdle prism such as right angle respectively, compression light path that left and right optical axis is turned back respectively
Physical dimension, the second total reflective mirror 3 and spectroscope 7 are at 45 ° respectively by T lines light path and C line light paths optical axis folding with optical axis
90 ° are returned, electric-controlled switch door 6 is located between the second total reflective mirror of T lines light path 3 and spectroscope 7, plays control T lines in time
The outgoing effect of fluorescence, dichroic mirror 8 is at 45 ° in the horizontal plane with optical axis, receiving light path the first optical filter 9, the second condenser lens
10th, aperture 11 and photodetector 12 are arranged in order behind dichroic mirror 8, and on excitation light path after dichroic mirror 8, second filters
Piece 13, collimation lens 14 and LED light source 15 are arranged in order.
First condenser lens 1, collimation lens 8, the optical surface of the second condenser lens 13 are coated with the anti-reflection medium of respective wavelength
Film.The isosceles fully reflecting surface of the girdle prisms such as right angle 5 is coated with respective wavelength all-dielectric film.First total reflective mirror 2, the second total reflective mirror 3,
The reflecting surface of 3rd total reflective mirror 4 is coated with respective wavelength all-dielectric film.The plane of incidence of spectroscope 7 is coated with respective wavelength 5:5 reflections
Deielectric-coating, exit facet are coated with the anti-reflection deielectric-coating of respective wavelength.The plane of incidence of dichroic mirror 8 is coated with the anti-reflection deielectric-coating of respective wavelength,
Exit facet is coated with the all-dielectric film of respective wavelength.
As shown in Figures 2 and 3, when carrying out fluoroscopic examination to C lines, the exciting light that LED light source 15 is sent passes through collimation lens
14 carry out collimation be collimated light beam, through the second optical filter 13 filter after, transmitted through dichroic mirror 8, be divided through spectroscope 7, swash all the way
It is luminous by reflection 90 ° reflexed to through total reflective mirror 4 on the right angle reflecting surface of the girdle prisms such as right angle 5, the right angle through the girdle prisms such as right angle 5
The C lines direction of fluorescence immunoassay test paper, another way are projected after reflective surface by the mirror body optically focused of the half of the first condenser lens 1
Exciting light transmitted through spectroscope 7 can not excite the T lines of test paper because electric-controlled switch door 6 is closed.C lines are excited the fluorescence sent
Dichroic mirror 8 is traveled to according to opposite direction, the first optical filter of receiving light path 9 is reflected to, passes through the second condenser lens after optical filtering
13, converge to aperture 11 and received by photodetector 12, the fluorescence now received is C line fluorescence intensities.
When carrying out fluoroscopic examination to T lines, it is flat that exciting light that LED light source 15 is sent carries out collimation by collimation lens 14
Row light beam, after the optical filtering of the second optical filter 13, transmitted through dichroic mirror 8, it is divided through spectroscope 7, exciting light is according to above-mentioned light all the way
Road deexcitation C lines, part exciting light project the second total reflective mirror 3 by the electric-controlled switch door 6 opened transmitted through spectroscope 7, passed through
90 ° of reflection projects the second total reflective mirror 2, then the right angle reflective surface through the girdle prisms such as right angle 5, passes through the first condenser lens 1
The mirror body optically focused of half projects the T lines direction of fluorescence immunoassay test paper.Two-way exciting light excites fluorescence on C lines and T lines respectively,
Fluorescence travels to dichroic mirror 8 by respective light path opposite direction, is reflected to the first optical filter of receiving light path 9, passes through after optical filtering
Second condenser lens 13, converge to aperture 11 and received by photodetector 12, the fluorescence now received is C lines and T lines
Fluorescence intensity sum.T line fluorescence intensities can be calculated through photoelectric signal collection, circuit signal processing.
From light emitting diode as fluorescence excitation light source, centre wavelength 400-500nm, 10mW；The dichroic of selection
Mirror has more than 98% high reflectance to light of the wavelength between 350nm-550nm, to wavelength between 570nm-850nm
Light has more than 90% high transmittance；First condenser lens selects balsaming lens, external diameter 12mm, focal length 10mm；First
Collimation lens selects planoconvex spotlight, external diameter 9.94mm, focal length 8mm；Second condenser lens selects planoconvex spotlight, external diameter
9.94mm, focal length 8mm；The a diameter of 12mm of fluorescence narrow band pass filter, thickness 2.0mm, half-band width is scholar 20nm, to centre wavelength
680nm fluorescence has 85% maximum transmission；Diaphragm has the function that to filter out veiling glare；Its response wave length of photodiode
Scope is 320nm-1100nm, and the receiving sensitivity to 800nm fluorescence is about 0.45, and its maximum reverse operating voltage is 30V, most
High-power is 50mW, and dark current normal value is 0.2nA.
Example uses homemade c- reactive proteins (CRP) chromatograph test strip of AoPu Co., Ltd, and the CAP for configuring 5mg/L concentration is molten
Liquid, obtain 5mg/L, 2.5mg/L, 1.12mg/L, 10 times of dilutions after half-and-half diluting and obtain 0.5mg/L, 0.25mg/L, 0.125mg/
The sample solution of L concentration, concentration limit of the present invention is 0.25mg/L after testing.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, although with reference to foregoing reality
The present invention is described in detail example, and for those skilled in the art, it still can be to foregoing each example institute
The technical scheme of record is modified, or carries out equivalent substitution to which part technical characteristic.It is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (7)
1. one kind light splitting detection means, it is characterised in that：It is all-trans including the first condenser lens (1), the first total reflective mirror (2), second
The girdle prisms (5) such as mirror (3), the 3rd total reflective mirror (4), right angle, electric-controlled switch door (6), spectroscope (7), dichroic mirror (8), the first filter
Mating plate (9), the second condenser lens (10), aperture (11), photodetector (12), the second optical filter (13), collimation lens
And LED light source (15) (14)；
Wherein, the girdle prism such as right angle (5) is located above the first condenser lens (1), and the axis of girdle prism such as right angle and first focuses on
The middle overlapping of axles of lens；First total reflective mirror (2), the 3rd total reflective mirror (4) are located at right angle etc. girdle prism (5) both sides, and respectively with directly
The right-angle surface of the girdle prisms such as angle (5) two is parallel；
Using the axis of the first condenser lens as optical axis, the second total reflective mirror (3) and spectroscope (7) and optical axis are at 45 °, the second total reflective mirror
(3) directly over the first total reflective mirror (2), spectroscope (7) is located at directly over the 3rd total reflective mirror (4), and electric-controlled switch door (6) is located at
Between second total reflective mirror (3) and spectroscope (7)；
Dichroic mirror (8) and optical axis are at 45 °, the first optical filter (9), the second condenser lens (10), aperture on receiving light path
(11) it is arranged in order with photodetector (12)；Second optical filter (13), collimation lens (14) and LED light source on excitation light path
(15) it is arranged in order.
2. light splitting detection means according to claim 1, it is characterised in that：When carrying out fluoroscopic examination to C lines, LED light source
(15) exciting light sent after collimation lens (14) and the second optical filter (13) by being transmitted to dichroic mirror (8), through dichroic mirror (8)
Reflex on spectroscope (7), be divided through spectroscope (7)；
Exciting light is reflexed on the right angle reflecting surface of the girdle prisms such as right angle (5) for 90 ° by reflection through total reflective mirror (4) all the way, through right angle
Fluorescence immunoassay is projected by the mirror body optically focused of the first condenser lens (1) side Deng after the right angle reflective surface of girdle prism (5)
The C lines direction of test paper；Another way exciting light can not excite test paper transmitted through spectroscope (7) because electric-controlled switch door (6) is closed
T lines；
The fluorescence that C lines are excited to send travels to dichroic mirror (8) according to opposite direction, is reflected to the first optical filter of receiving light path
(9), converge to aperture (11) by the second condenser lens (10) after optical filtering and received by photodetector (12).
3. light splitting detection means according to claim 1, it is characterised in that：When carrying out fluoroscopic examination to T lines, LED light source
(15) exciting light sent by after collimation lens (14) and the second optical filter (13) transmitted through dichroic mirror (8), through dichroic mirror (8)
Reflex on spectroscope (7), be divided through spectroscope (7)；
Exciting light is reflexed on the right angle reflecting surface of the girdle prisms such as right angle (5) for 90 ° by reflection through total reflective mirror (4) all the way, through right angle
Fluorescence immunoassay is projected by the mirror body optically focused of the first condenser lens (1) side Deng after the right angle reflective surface of girdle prism (5)
The C lines direction of test paper；
The fluorescence that C lines are excited to send travels to dichroic mirror (8) according to opposite direction, is reflected to the first optical filter of receiving light path
(9), converge to aperture (11) by the second condenser lens (10) after optical filtering and received by photodetector (12)；
For another way exciting light transmitted through spectroscope (7), the electric-controlled switch door (6) by opening projects the second total reflective mirror (3), warp
90 ° of reflection projects the first total reflective mirror (2), then the right angle reflective surface through the girdle prisms such as right angle (5), is focused on by first saturating
The mirror body optically focused of mirror (1) side projects the T lines direction of fluorescence immunoassay test paper；
The fluorescence that T lines are excited to send travels to dichroic mirror (8) according to opposite direction, is reflected to the first optical filter of receiving light path
(9), converge to aperture (11) by the second condenser lens (10) after optical filtering and received by photodetector (12)；
The fluorescence that photodetector (12) receives is C lines and T line fluorescence intensity sums.
4. light splitting detection means according to claim 1, it is characterised in that：First condenser lens (1), collimation lens
(14), the optical surface of the second condenser lens (10) is coated with anti-reflection deielectric-coating.
5. light splitting detection means according to claim 1, it is characterised in that：First total reflective mirror (2), the second total reflective mirror
(3), the isosceles fully reflecting surface of the girdle prism (5) such as the reflecting surface of the 3rd total reflective mirror (4) and right angle is coated with all-dielectric film.
6. light splitting detection means according to claim 1, it is characterised in that：The plane of incidence of the spectroscope (7) is coated with 5:5
Reflecting medium film, exit facet are coated with anti-reflection deielectric-coating.
7. light splitting detection means according to claim 1, it is characterised in that：The plane of incidence of the dichroic mirror (8) is coated with increasing
Saturating deielectric-coating, exit facet are coated with all-dielectric film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710981298.2A CN107561012B (en) | 2017-10-20 | 2017-10-20 | Spectroscopic detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710981298.2A CN107561012B (en) | 2017-10-20 | 2017-10-20 | Spectroscopic detection device |
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| CN107561012A true CN107561012A (en) | 2018-01-09 |
| CN107561012B CN107561012B (en) | 2023-08-01 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7489403B1 (en) * | 2007-11-26 | 2009-02-10 | Kaiwood Technology Co., Ltd. | Electronic testing apparatus and testing method thereof |
| CN102253015A (en) * | 2011-03-23 | 2011-11-23 | 中国科学院上海光学精密机械研究所 | Embedded immunochromatography fluorescence detection system and detection method |
| CN103557940A (en) * | 2013-10-24 | 2014-02-05 | 杭州远方光电信息股份有限公司 | Spectrograph |
| CN106546728A (en) * | 2015-09-16 | 2017-03-29 | 杨波 | A kind of new immunochromatography reagent bar and its technical scheme of detection |
| CN207300860U (en) * | 2017-10-20 | 2018-05-01 | 南京先进激光技术研究院 | It is divided detection device |
-
2017
- 2017-10-20 CN CN201710981298.2A patent/CN107561012B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7489403B1 (en) * | 2007-11-26 | 2009-02-10 | Kaiwood Technology Co., Ltd. | Electronic testing apparatus and testing method thereof |
| CN102253015A (en) * | 2011-03-23 | 2011-11-23 | 中国科学院上海光学精密机械研究所 | Embedded immunochromatography fluorescence detection system and detection method |
| CN103557940A (en) * | 2013-10-24 | 2014-02-05 | 杭州远方光电信息股份有限公司 | Spectrograph |
| CN106546728A (en) * | 2015-09-16 | 2017-03-29 | 杨波 | A kind of new immunochromatography reagent bar and its technical scheme of detection |
| CN207300860U (en) * | 2017-10-20 | 2018-05-01 | 南京先进激光技术研究院 | It is divided detection device |
Non-Patent Citations (1)
| Title |
|---|
| 潘星等: "免疫荧光定量分析仪设计", 《嵌入式技术》 * |
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