CN106546728A - A kind of new immunochromatography reagent bar and its technical scheme of detection - Google Patents
A kind of new immunochromatography reagent bar and its technical scheme of detection Download PDFInfo
- Publication number
- CN106546728A CN106546728A CN201510589025.4A CN201510589025A CN106546728A CN 106546728 A CN106546728 A CN 106546728A CN 201510589025 A CN201510589025 A CN 201510589025A CN 106546728 A CN106546728 A CN 106546728A
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- Prior art keywords
- detection
- reagent strip
- reagent
- detecting instrument
- lines
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6447—Fluorescence; Phosphorescence by visual observation
Abstract
The present invention relates to the technical scheme of a kind of new immunochromatography reagent bar and its detection, mainly solves current immunochromatography reagent and its detection technique scheme is unfavorable for the further volume miniaturization of detecting instrument, cost-effective and further improves the technical problem of detection accuracy.By being opened the light according to hole and peep hole respectively from transparent test strips base plate and in the shell upper and lower covers position of detection zone, make the light source assembly and signal detection component of supporting detecting instrument operationally be located at reagent strip both sides respectively, change whole light channel structure.The light path design of detecting instrument is this solution simplifies, is allow light source, two core parts of signal sensor very close to reagent strip, is under equal conditions reduced volume, reduce cost, improve detection sensitivity and accuracy.
Description
Technical field
The present invention relates to the technical scheme of a kind of new immunochromatography reagent bar and its detection, more particularly to a kind of immunochromatography of application fluoroscopic examination
Reagent strip and its technical scheme of detection.In terms of can be applicable to in-vitro diagnosis, food security and illicit drugs inspection, particularly in terms of POCT quick diagnosis.
Technical background
Immunochromatography technique is a kind of Measurement for Biotechnique designed according to the mutual absorption principle and capillarity of immunizing antigen-antibody, currently
It is widely used in in-vitro diagnosis, food security and illicit drugs inspection, particularly quickly grows in terms of quick diagnosis (POCT).Chromatography passes through solid phase
During form solves immunoassay process, different material (particularly determinand and impurity) is separated, and the reagent components needed for immunoreaction process are carried
Before be cured to the relevant position of reagent strip, the step of so as to greatly simplify detection, reagent strip solid phase is easy to maintain.It is that the information of determinand amount is converted into
The signal that can be detected by corresponding instrument equipment, the general signal that the forms such as light, electricity, magnetic are translated into using biomarker technology, wherein being mainly
Optical signal.According to the difference of label, different classification, such as gold-marking immunity chromatography, fluorescence immune chromatography, time-resolved fluoroimmunoassay chromatography are produced
Deng.The product formed using immunochromatography technique generally comprises two parts：Chromatography reagent strip and supporting detecting instrument.The latter is provided for the former reaction
Environment-guarantee, create luminescent condition, complete reading Analysis etc..Both constitute a complete test system at combination.
Currently, the structure for chromatographing reagent strip is that test paper is constituted with shell.On the base plate of White-opalescent, according to each needing, patch is viscous to be loaded pad, knot
Close pad, immobilon-p, adsorptive pads etc. and form test paper.Immobilon-p, generally NC films, have drawn T line C lines thereon.Shell lower cover (presses close to base plate one
Side) without perforate, and it is upper lid at least sample-adding pad, immobilon-p correspondence position opens sample well and peep hole is each one.Add necessarily in sample well during test
The sample of amount, Jing are chromatographed for a period of time and are completed, and irradiate the incident light of specific wavelength in peep hole side, and incident light illuminates the T line C lines of test paper, or
Make its colour developing or make which excite generation fluorescence, while colourity or fluorescence intensity are read in peep hole side.I.e. lamp source and detecting component is in reagent strip side.
Two light path part is overlapped, the interference of device Existential Space.Flashlight and light source veiling glare etc. will be distinguished simultaneously, be that this need to design relative complex light path,
Make light source and sensitive detection parts from test paper T line C lines farther out.This causes：(1) space is larger；(2) sensitivity is damaged；(3) high expensive.
Quick diagnosis require compact portable, the cheap practicality of detecting system.Current immunochromatography reagent bar and its detection method are limited and are further reduced
Equipment instrument, reduces cost.
The content of the invention
The invention aims to make further to be miniaturized based on the detecting system of immuno-chromatographic test paper strip, improve sensitivity reduces cost.
The present invention core be：1) base plate of the test strips using transparent material；2) it is upper and lower two in the shell of the T line C line correspondence positions of test strips
Divide and respectively open a hole, a hole is used for illumination, a hole is used to detect；3) light source assembly in supporting detecting instrument, signal detection component are located at examination respectively
Agent bar both sides, and as close to reagent strip.
As the light source assembly of supporting detecting instrument is located at reagent strip both sides respectively with signal detection component, exciting light is non-overlapping with fluorescence light path, does not deposit
More simplify with the design of signal detection component in the interference in space, therefore the light source assembly of necessary instrument, or even basic light requirement source and signal detection
Device (such as photodiode, PMT etc.).So optical light source and detector can as close to reagent strip to improve the collection efficiency of light efficiency and signal,
So not only reduce volume and can also improve detection sensitivity, while reducing cost.
Description of the drawings
Accompanying drawing is the schematic diagram of technical solution of the present invention.Reagent strip is made up of outer casing upper cover 1, test strips 2 and shell lower cover 3.Light source assembly 4 with
Signal detection component 5 is the important component part of supporting detecting instrument, completes the collection to reagent bars.
Sample well 6 and illumination hole 7 are opened on outer casing upper cover typically.
Test strips part is similar to traditional immuno-chromatographic test paper strip, by sample pad and pad 8, adsorptive pads 9, immobilon-p 10 and dianegative 11
Composition, spray on immobilon-p draw T lines 12 and C lines 13.Immobilon-p is generally NC films, it is possible to use the film of other materials.By special equipment solid
Spray on phase film marks T lines and C lines, and sometimes for increasing test event or improving the accuracy of test, spray marks a few road T lines or C lines.Sample pad
And pad, can typically separate independent sample pad and combination mat.But portioned product is in advance by blood sample and labelled antibody hybrid reaction, then test paper part
Pad is eliminated just.
Increase peep hole 14 on shell lower cover.
The exciting light 15 that the light source assembly of detecting instrument sends is irradiated to the detection zone of test strips by upper lid illumination hole, i.e., all T lines C lines
Region, excites the label in the region to send fluorescence 16.Fluorescence, through dianegative, reaches the detection group of detecting instrument by the peep hole of lower cover
Part.
Specific embodiment
Accompanying drawing and its explanation have clearly scanned embodiments of the present invention, i.e., using test strips part using dianegative, reagent strip shell it is upper
Lower cover is respectively opened when a hole, the light source assembly of detecting instrument and probe assembly work in detection zone correspondence position and is arranged in reagent strip both sides.
The fluorescence that the present invention is referred to, be not limited only to conventional fluorescent element mark fluorescence immunoassay, be also applied for time-resolved fluorescence, on turn chemiluminescence etc..
They all excite the light for making label etc. produce another kind of wavelength using a kind of light of wavelength, and the amount of the latter's intensity and determinand is closed into certain mathematics
System.
The purpose that the present invention makes base plate transparent can be to detect fluorescence signal in opposite side, so " transparent " is for fluorescence, refer to the ripple
Long light has very high transmissivity.Simultaneously base plate can also be only in detection zone location transparency, even if place is opaque nor affects on the present invention for other
Enforcement.
The present invention can shorten the stroke of fluorescence to sensitive detection parts, improve phosphor collection efficiency.Convex lens in probe assembly etc. can consider to delete,
But optical filter suggestion preserves to reduce the interference of other bias lights.
Light source assembly in the present invention, it is possible to use the device such as convex lens further improves the utilization rate of light source, improves the special of wavelength using optical filter
Property.But advise preferentially using single light source in implementation process.
Separation reagent both sides when light source assembly in the present invention is worked with probe assembly, but do not require the two point-blank.Advise in real process
The two forms an angle, in order to avoid too strong lit transmissive test paper interference detection effect.
Claims (5)
1. the present invention is the technical scheme of a kind of new immunochromatography reagent bar and its detection, it is characterised in that：The base plate of test strips is transparent material, its
Shell upper and lower covers open a hole in detection zone (covering all T lines C lines parts) corresponding part, and the light source assembly of supporting detecting instrument and signal are visited
Component is surveyed respectively positioned at the both sides of reagent strip and as close to reagent strip.
2. test strips as claimed in claim 1, it is characterised in that：The transparent material of the base plate is, for detectable signal, to refer to and can visit
Survey signal and can penetrate the material.
3. detecting instrument as claimed in claim 1, it is characterised in that：Excitation source component and photoelectric sensor assembly are operationally respectively positioned at reagent strip
Both sides, both not necessarily point-blank, can form an angle arrangement.
4. reagent strip as claimed in claim 1, it is characterised in that：The position shape of the shell upper and lower covers perforate, is according to ELISA test strip region
Position shape design, its size should cover whole detection zone, i.e., all T lines C lines parts, but should not be too many beyond detection zone.
5. reagent strip as claimed in claim 1 or 2, it is characterised in that：The dianegative can be whole base plate, it is also possible to only in detection zone
Correspondence position is transparent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510589025.4A CN106546728A (en) | 2015-09-16 | 2015-09-16 | A kind of new immunochromatography reagent bar and its technical scheme of detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510589025.4A CN106546728A (en) | 2015-09-16 | 2015-09-16 | A kind of new immunochromatography reagent bar and its technical scheme of detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106546728A true CN106546728A (en) | 2017-03-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510589025.4A Pending CN106546728A (en) | 2015-09-16 | 2015-09-16 | A kind of new immunochromatography reagent bar and its technical scheme of detection |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561012A (en) * | 2017-10-20 | 2018-01-09 | 南京先进激光技术研究院 | It is divided detection means |
| CN110927136A (en) * | 2019-12-25 | 2020-03-27 | 杭州微策生物技术有限公司 | Quality detection device of dry-type immunofluorescence POCT detection instrument and use method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040191119A1 (en) * | 2003-03-24 | 2004-09-30 | Zanzucchi Peter J. | Analyte concentration detection devices and methods |
| CN101467027A (en) * | 2006-06-15 | 2009-06-24 | 皇家飞利浦电子股份有限公司 | Integrated biosensing device having photo detector |
| CN101696447A (en) * | 2009-11-16 | 2010-04-21 | 上海交通大学 | Test strip for testing psychrophiles in chilled foods and preparation method thereof |
| CN103777003A (en) * | 2014-01-27 | 2014-05-07 | 西南大学 | Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof |
| CN104833799A (en) * | 2014-02-07 | 2015-08-12 | 恩智浦有限公司 | Analyte detection methods and devices |
-
2015
- 2015-09-16 CN CN201510589025.4A patent/CN106546728A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040191119A1 (en) * | 2003-03-24 | 2004-09-30 | Zanzucchi Peter J. | Analyte concentration detection devices and methods |
| CN101467027A (en) * | 2006-06-15 | 2009-06-24 | 皇家飞利浦电子股份有限公司 | Integrated biosensing device having photo detector |
| CN101696447A (en) * | 2009-11-16 | 2010-04-21 | 上海交通大学 | Test strip for testing psychrophiles in chilled foods and preparation method thereof |
| CN103777003A (en) * | 2014-01-27 | 2014-05-07 | 西南大学 | Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof |
| CN104833799A (en) * | 2014-02-07 | 2015-08-12 | 恩智浦有限公司 | Analyte detection methods and devices |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561012A (en) * | 2017-10-20 | 2018-01-09 | 南京先进激光技术研究院 | It is divided detection means |
| CN110927136A (en) * | 2019-12-25 | 2020-03-27 | 杭州微策生物技术有限公司 | Quality detection device of dry-type immunofluorescence POCT detection instrument and use method thereof |
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