CN207300860U - It is divided detection device - Google Patents
It is divided detection device Download PDFInfo
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- CN207300860U CN207300860U CN201721353369.6U CN201721353369U CN207300860U CN 207300860 U CN207300860 U CN 207300860U CN 201721353369 U CN201721353369 U CN 201721353369U CN 207300860 U CN207300860 U CN 207300860U
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- mirror
- total reflective
- right angle
- reflective mirror
- condenser lens
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Abstract
The utility model discloses one kind to be divided detection device, is made of girdle prism, electric-controlled switch door, spectroscope, dichroic mirror, the first optical filter, collimation lens, LED light source, the second optical filter, the second condenser lens, aperture and photodetectors such as the first condenser lens, the first total reflective mirror, the second total reflective mirror, the 3rd total reflective mirror, right angles.The utility model device can be realized excites adjacent several millimeters of fluorometric reagent line arranged side by side respectively, and collect the respective fluorescence intensity of detection, interfering with each other for fluorescent test paper T lines and C line fluorescence can be excluded completely, which has the characteristics that compact-sized, small, stability is good.
Description
Technical field
A kind of fluorescence detection device of hand-held immunity analysis instrument is the utility model is related to, more particularly to it is a kind of closely glimmering
The light splitting detection device of striations detection.
Background technology
The research of fluorescence immunoassay instrument device can realize the quantification detection to test strips using own warp, but at present
Most instruments obtain C lines and T line optical signal intensity using the method for optical mechaical scanning, and equipment instrument is excessive, is not easy to
Carrying and Site Detection, constrain the application and popularization of this technology.
There are implacable lance with quantitative accuracy of detection raising for existing fluorescence immunoassay instrument device volume-diminished
Shield.
Utility model content
Goal of the invention：In view of the above problems, the utility model proposes one kind to be divided detection device, by C in optical design
Line and T linear light signals physically separate.
Technical solution：To realize above-mentioned purpose of design, technical solution is used by the utility model：One kind light splitting detection
Device, including girdle prism, the electric-controlled switch such as the first condenser lens, the first total reflective mirror, the second total reflective mirror, the 3rd total reflective mirror, right angle
Door, spectroscope, dichroic mirror, the first optical filter, the second condenser lens, aperture, photodetector, the second optical filter, collimation
Lens and LED light source；Wherein, the girdle prism such as right angle is located above the first condenser lens, the axis of girdle prism and first such as right angle
The middle overlapping of axles of condenser lens；First total reflective mirror, the 3rd total reflective mirror are located at the girdle prism both sides such as right angle, and respectively with right angle isosceles
Two right-angle surface of prism is parallel；Using the axis of the first condenser lens as optical axis, the second total reflective mirror and spectroscope and optical axis are at 45 °, and
Two total reflective mirrors are located at directly over the first total reflective mirror, and spectroscope is located at directly over the 3rd total reflective mirror, and electric-controlled switch door is complete positioned at second
Between anti-mirror and spectroscope；Dichroic mirror is at 45 ° with optical axis, the first optical filter, the second condenser lens, aperture on receiving light path
It is arranged in order with photodetector；The second optical filter, collimation lens and LED light source are arranged in order on excitation light path.
When carrying out fluoroscopic examination to C lines, the exciting light that LED light source is sent after collimation lens and the second optical filter by transmiting
To dichroic mirror, reflex on spectroscope through dichroic mirror, be divided through spectroscope；Exciting light is reflected for 90 ° by reflection through total reflective mirror all the way
Onto the right angle reflecting surface of the girdle prisms such as right angle, pass through the first condenser lens after the right angle reflective surface of the girdle prisms such as right angle
The mirror body optically focused of side projects the C lines direction of fluorescence immunoassay test paper；Another way exciting light is opened transmitted through spectroscope due to automatically controlled
Close the door and close and the T lines of test paper can not be excited；The fluorescence that C lines are excited to send travels to dichroic mirror according to opposite direction, is reflected to
First optical filter of receiving light path, by the second condenser lens after optical filtering, converges to aperture and is received by a photoelectric detector.
When carrying out fluoroscopic examination to T lines, the exciting light that LED light source is sent after collimation lens and the second optical filter by transmiting
Dichroic mirror is crossed, is reflexed to through dichroic mirror on spectroscope, is divided through spectroscope；Exciting light is reflected for 90 ° by reflection through total reflective mirror all the way
Onto the right angle reflecting surface of the girdle prisms such as right angle, pass through the first condenser lens after the right angle reflective surface of the girdle prisms such as right angle
The mirror body optically focused of side projects the C lines direction of fluorescence immunoassay test paper；The fluorescence that C lines are excited to send travels to two according to opposite direction
Look mirror, is reflected to the first optical filter of receiving light path, by the second condenser lens after optical filtering, converges to aperture by photoelectricity
Detector receives；Another way exciting light is transmitted through spectroscope, and the electric-controlled switch door by opening projects the second total reflective mirror, through anti-
Penetrate 90 ° and project the first total reflective mirror, then the right angle reflective surface through girdle prisms such as right angles, pass through the first condenser lens side
Mirror body optically focused projects the T lines direction of fluorescence immunoassay test paper；The fluorescence that T lines are excited to send travels to dichroic mirror according to opposite direction,
The first optical filter of receiving light path is reflected to, by the second condenser lens after optical filtering, converges to aperture by photodetection
Device receives；The fluorescence that photodetector receives is the sum of C lines and T line fluorescence intensities.
Beneficial effect：The utility model overcomes existing fluorescence immunoassay instrument device volume-diminished and quantitative detection essence
Degree improves existing contradiction, high certainty of measurement, easy to carry and Site Detection, there is provided one kind is in optical design by C lines
With T linear lights signal physically separated detection device, it can be used for the research and development of fluorescence immunoassay instrument device.
The utility model device can be realized excites adjacent several millimeters of fluorometric reagent line arranged side by side respectively, and receives
Collection detects respective fluorescence intensity, can exclude interfering with each other for fluorescent test paper T lines and C line fluorescence completely, which has knot
The features such as structure is compact, small, stability is good.
Brief description of the drawings
Fig. 1 is the explosive view for being divided detection device；
Fig. 2 is the front view for being divided detection device light path；
Fig. 3 is the top view for being divided detection device light path.
Embodiment
The technical solution of the utility model is further described with reference to the accompanying drawings and examples.
As shown in Figure 1, the light splitting detection device of the utility model, is detected applied to closely fluorescence striped, including first
The girdle prisms 5 such as condenser lens 1, the first total reflective mirror 2, the second total reflective mirror 3, the 3rd total reflective mirror 4, right angle, electric-controlled switch door 6, light splitting
Mirror 7, dichroic mirror 8, the second optical filter 13, collimation lens 14, LED light source 15, the first optical filter 9, the second condenser lens 10, aperture
Diaphragm 11 and photodetector 12.
As shown in Figures 2 and 3, the middle overlapping of axles of the girdle prism 5 such as the axis of the first condenser lens 1 and right angle, ensures with right angle
T lines light path and C line light paths are divided into the left and right sides by the rectangular edge Deng girdle prism 5 for light path line of demarcation, and first is complete
Anti- mirror 2, the 3rd total reflective mirror 4 are parallel with 5 liang of right-angle surfaces of girdle prism such as right angle respectively, compression light path that left and right optical axis is turned back respectively
Structure size, the second total reflective mirror 3 and spectroscope 7 are at 45 ° respectively by T lines light path and C line light paths optical axis folding with optical axis
90 ° are returned, electric-controlled switch door 6 is located between the second total reflective mirror of T lines light path 3 and spectroscope 7, plays control T lines in time
The outgoing effect of fluorescence, dichroic mirror 8 is at 45 ° in the horizontal plane with optical axis, receiving light path the first optical filter 9, the second condenser lens
10th, aperture 11 and photodetector 12 are arranged in order behind dichroic mirror 8, and on excitation light path after dichroic mirror 8, second filters
Piece 13, collimation lens 14 and LED light source 15 are arranged in order.
First condenser lens 1, collimation lens 8, the optical surface of the second condenser lens 13 are coated with the anti-reflection medium of respective wavelength
Film.The isosceles fully reflecting surface of the girdle prisms such as right angle 5 is coated with respective wavelength all-dielectric film.First total reflective mirror 2, the second total reflective mirror 3,
The reflecting surface of 3rd total reflective mirror 4 is coated with respective wavelength all-dielectric film.The plane of incidence of spectroscope 7 is coated with respective wavelength 5:5 reflections
Deielectric-coating, exit facet are coated with the anti-reflection deielectric-coating of respective wavelength.The plane of incidence of dichroic mirror 8 is coated with the anti-reflection deielectric-coating of respective wavelength,
Exit facet is coated with the all-dielectric film of respective wavelength.
As shown in Figures 2 and 3, when carrying out fluoroscopic examination to C lines, the exciting light that LED light source 15 is sent passes through collimation lens
14 carry out collimation be collimated light beam, through the second optical filter 13 filter after, transmitted through dichroic mirror 8, be divided through spectroscope 7, swash all the way
Shine and reflexed to by reflection through total reflective mirror 4 on the right angle reflecting surface of the girdle prisms such as right angle 5 for 90 °, the right angle through the girdle prisms such as right angle 5
The C lines direction of fluorescence immunoassay test paper, another way are projected after reflective surface by the mirror body optically focused of 1 half of the first condenser lens
Exciting light transmitted through spectroscope 7 can not excite the T lines of test paper since electric-controlled switch door 6 is closed.C lines are excited the fluorescence sent
Dichroic mirror 8 is traveled to according to opposite direction, the first optical filter of receiving light path 9 is reflected to, passes through the second condenser lens after optical filtering
13, converge to aperture 11 and received by photodetector 12, the fluorescence received at this time is C line fluorescence intensities.
When carrying out fluoroscopic examination to T lines, it is flat that exciting light that LED light source 15 is sent carries out collimation by collimation lens 14
Row light beam, after the optical filtering of the second optical filter 13, transmitted through dichroic mirror 8, is divided, exciting light is according to above-mentioned light all the way through spectroscope 7
Road deexcitation C lines, part exciting light project the second total reflective mirror 3 by the electric-controlled switch door 6 opened transmitted through spectroscope 7, pass through
90 ° of reflection projects the second total reflective mirror 2, then the right angle reflective surface through the girdle prisms such as right angle 5, passes through the first condenser lens 1
The mirror body optically focused of half projects the T lines direction of fluorescence immunoassay test paper.Two-way exciting light excites fluorescence on C lines and T lines respectively,
Fluorescence travels to dichroic mirror 8 by respective light path opposite direction, is reflected to the first optical filter of receiving light path 9, passes through after optical filtering
Second condenser lens 13, converges to aperture 11 and is received by photodetector 12, and the fluorescence received at this time is C lines and T lines
The sum of fluorescence intensity.T line fluorescence intensities can be calculated through photoelectric signal collection, circuit signal processing.
Light emitting diode is selected as fluorescence excitation light source, centre wavelength 400-500nm, 10mW；The dichroic of selection
Mirror has light of the wavelength between 350nm-550nm more than 98% high reflectance, to wavelength between 570nm-850nm
Light has more than 90% high transmittance；First condenser lens selects balsaming lens, outside diameter 12mm, focal length 10mm；First
Collimation lens selects planoconvex spotlight, outside diameter 9.94mm, focal length 8mm；Second condenser lens selects planoconvex spotlight, outside diameter
9.94mm, focal length 8mm；The a diameter of 12mm of fluorescence narrow band pass filter, thickness 2.0mm, half-band width is scholar 20nm, to centre wavelength
The fluorescence of 680nm has 85% maximum transmission；Diaphragm has the function that to filter out veiling glare；Its response wave length of photodiode
Scope is 320nm-1100nm, and the receiving sensitivity to 800nm fluorescence is about 0.45, its maximum reverse operating voltage is 30V, most
High-power is 50mW, and dark current normal value is 0.2nA.
Example uses homemade c- reactive proteins (CRP) chromatograph test strip of AoPu Co., Ltd, and the CAP for configuring 5mg/L concentration is molten
Liquid, obtains 5mg/L, 2.5mg/L, 1.12mg/L, 10 times of dilutions after half-and-half diluting and obtains 0.5mg/L, 0.25mg/L, 0.125mg/
The sample solution of L concentration, concentration limit of the present invention is 0.25mg/L after testing.
The above descriptions are merely preferred embodiments of the present invention, is not intended to limit the present invention, although ginseng
The utility model is described in detail according to previous examples, for those skilled in the art, it still can be right
Technical solution described in foregoing each example is modified, or carries out equivalent substitution to which part technical characteristic.It is all in reality
Within new spirit and principle, any modification, equivalent replacement, improvement and so on, should be included in the utility model
Within protection domain.
Claims (7)
1. one kind light splitting detection device, it is characterised in that：It is all-trans including the first condenser lens (1), the first total reflective mirror (2), second
The girdle prisms (5) such as mirror (3), the 3rd total reflective mirror (4), right angle, electric-controlled switch door (6), spectroscope (7), dichroic mirror (8), the first filter
Mating plate (9), the second condenser lens (10), aperture (11), photodetector (12), the second optical filter (13), collimation lens
(14) and LED light source (15)；
Wherein, the girdle prism such as right angle (5) is located above the first condenser lens (1), and the axis of girdle prism such as right angle and first focuses on
The middle overlapping of axles of lens；First total reflective mirror (2), the 3rd total reflective mirror (4) are located at right angle etc. girdle prism (5) both sides, and respectively with directly
(5) two right-angle surface of the girdle prisms such as angle is parallel；
Using the axis of the first condenser lens as optical axis, the second total reflective mirror (3) and spectroscope (7) and optical axis are at 45 °, the second total reflective mirror
(3) directly over the first total reflective mirror (2), spectroscope (7) is located at directly over the 3rd total reflective mirror (4), and electric-controlled switch door (6) is located at
Between second total reflective mirror (3) and spectroscope (7)；
Dichroic mirror (8) and optical axis are at 45 °, the first optical filter (9), the second condenser lens (10), aperture on receiving light path
(11) it is arranged in order with photodetector (12)；Second optical filter (13), collimation lens (14) and LED light source on excitation light path
(15) it is arranged in order.
2. light splitting detection device according to claim 1, it is characterised in that：When carrying out fluoroscopic examination to C lines, LED light source
(15) exciting light sent after collimation lens (14) and the second optical filter (13) by being transmitted to dichroic mirror (8), through dichroic mirror (8)
Reflex on spectroscope (7), be divided through spectroscope (7)；
Exciting light is reflexed on the right angle reflecting surface of the girdle prisms such as right angle (5) for 90 ° by reflection through total reflective mirror (4) all the way, through right angle
Fluorescence immunoassay is projected by the mirror body optically focused of the first condenser lens (1) side Deng after the right angle reflective surface of girdle prism (5)
The C lines direction of test paper；Another way exciting light can not excite test paper transmitted through spectroscope (7) since electric-controlled switch door (6) is closed
T lines；
The fluorescence that C lines are excited to send travels to dichroic mirror (8) according to opposite direction, is reflected to the first optical filter of receiving light path
(9), converge to aperture (11) by the second condenser lens (10) after optical filtering and received by photodetector (12).
3. light splitting detection device according to claim 1, it is characterised in that：When carrying out fluoroscopic examination to T lines, LED light source
(15) exciting light sent by after collimation lens (14) and the second optical filter (13) transmitted through dichroic mirror (8), through dichroic mirror (8)
Reflex on spectroscope (7), be divided through spectroscope (7)；
Exciting light is reflexed on the right angle reflecting surface of the girdle prisms such as right angle (5) for 90 ° by reflection through total reflective mirror (4) all the way, through right angle
Fluorescence immunoassay is projected by the mirror body optically focused of the first condenser lens (1) side Deng after the right angle reflective surface of girdle prism (5)
The C lines direction of test paper；
The fluorescence that C lines are excited to send travels to dichroic mirror (8) according to opposite direction, is reflected to the first optical filter of receiving light path
(9), converge to aperture (11) by the second condenser lens (10) after optical filtering and received by photodetector (12)；
For another way exciting light transmitted through spectroscope (7), the electric-controlled switch door (6) by opening projects the second total reflective mirror (3), warp
90 ° of reflection projects the first total reflective mirror (2), then the right angle reflective surface through the girdle prisms such as right angle (5), is focused on by first saturating
The mirror body optically focused of mirror (1) side projects the T lines direction of fluorescence immunoassay test paper；
The fluorescence that T lines are excited to send travels to dichroic mirror (8) according to opposite direction, is reflected to the first optical filter of receiving light path
(9), converge to aperture (11) by the second condenser lens (10) after optical filtering and received by photodetector (12)；
The fluorescence that photodetector (12) receives is the sum of C lines and T line fluorescence intensities.
4. light splitting detection device according to claim 1, it is characterised in that：First condenser lens (1), collimation lens
(14), the optical surface of the second condenser lens (10) is coated with anti-reflection deielectric-coating.
5. light splitting detection device according to claim 1, it is characterised in that：First total reflective mirror (2), the second total reflective mirror
(3), the isosceles fully reflecting surface of the girdle prism (5) such as the reflecting surface of the 3rd total reflective mirror (4) and right angle is coated with all-dielectric film.
6. light splitting detection device according to claim 1, it is characterised in that：The plane of incidence of the spectroscope (7) is coated with 5:5
Reflecting medium film, exit facet are coated with anti-reflection deielectric-coating.
7. light splitting detection device according to claim 1, it is characterised in that：The plane of incidence of the dichroic mirror (8) is coated with increasing
Saturating deielectric-coating, exit facet are coated with all-dielectric film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721353369.6U CN207300860U (en) | 2017-10-20 | 2017-10-20 | It is divided detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721353369.6U CN207300860U (en) | 2017-10-20 | 2017-10-20 | It is divided detection device |
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| Publication Number | Publication Date |
|---|---|
| CN207300860U true CN207300860U (en) | 2018-05-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721353369.6U Withdrawn - After Issue CN207300860U (en) | 2017-10-20 | 2017-10-20 | It is divided detection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561012A (en) * | 2017-10-20 | 2018-01-09 | 南京先进激光技术研究院 | It is divided detection means |
| CN110794529A (en) * | 2020-01-06 | 2020-02-14 | 成都新易盛通信技术股份有限公司 | Optical assembly and system thereof |
-
2017
- 2017-10-20 CN CN201721353369.6U patent/CN207300860U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561012A (en) * | 2017-10-20 | 2018-01-09 | 南京先进激光技术研究院 | It is divided detection means |
| CN110794529A (en) * | 2020-01-06 | 2020-02-14 | 成都新易盛通信技术股份有限公司 | Optical assembly and system thereof |
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| AV01 | Patent right actively abandoned | ||
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Granted publication date: 20180501 Effective date of abandoning: 20230801 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20180501 Effective date of abandoning: 20230801 |