CN104198452A - Signal enhancement laser-induced fluorescence system - Google Patents
Signal enhancement laser-induced fluorescence system Download PDFInfo
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- CN104198452A CN104198452A CN201410464884.6A CN201410464884A CN104198452A CN 104198452 A CN104198452 A CN 104198452A CN 201410464884 A CN201410464884 A CN 201410464884A CN 104198452 A CN104198452 A CN 104198452A
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Abstract
The invention relates to a signal enhancement laser-induced fluorescence system. The signal enhancement laser-induced fluorescence system comprises a laser exciation light path and a fluorescence receiving light path, wherein the laser exciation light path comprises a laser device and a laser focus lens, and the fluorescence receiving light path comprises a sample room, a parabolic reflector, a focus lens, an optical fiber collimating lens, an optical fiber and a spectrograph in sequence; an axial hole for the sample room to come in and out is formed in the parabolic reflector in the axis direction, a hole for a laser beam to pass through is further formed in the paraboloid of the parabolic reflector and is vertical to the axis of the paraboloid, and the focus of the paraboloid is on the extension line of the hole; the focus of the parabolic reflector is positioned on the focus point of the laser focus lens, a sample in the sample room is positioned on the focus of the parabolic reflector, fluorescent light emitted after the sample is excitated is reflected by the parabolic reflector to form parallel light beams, and the parallel light beams are focused and coupled into the optical fiber through the focus lens and the optical fiber collimating lens and are finally captured by the spectrograph. The system can greatly improve the collection capacity and utilization rate of fluorescence signals, the fluorescence signals are enhanced, and thus the detection sensitivity of fluorescent light is improved.
Description
Technical field
The present invention relates to a kind of laser-induced fluorescence (LIF) system detecting for fluorescence signal, this system can effectively improve fluorescence signal capacity gauge, and high fluorescence signal utilization factor is provided, thereby strengthens fluorescence signal, and then improves the sensitivity of fluoroscopic examination.
Background technology
Fluorescence is a kind of photoluminescence phenomenon, and fluorescence spectrum is corresponding to the corresponding electronic energy level structure of material.Fluorescence analysis is owing to having good sensitivity and compared with low detectability and can use popular instrument to be widely used in agricultural, industry, health, medicine, judicial expertise and scientific research.
Fluorescence spectrum can be for the qualitative and quantitative analysis of material, the plurality of advantages such as that fluorescence spectrum has is highly sensitive, detection limit is low, operative installations is simple and sample dosage is few, but also have its limitation, many materials itself can not send fluorescence or the very weak fluorescence spectrum that cannot use of fluorescence that sends is analyzed it.In order to address this problem, fluorescence probe arises at the historic moment.So-called fluorescence probe is exactly that some has compared with the material of hyperfluorescenceZeng Yongminggaoyingguang character, thus people by these materials with non-fluorescence or hypofluorescence material links up the fluorescigenic complex compound of formation by covalent bond or other form and aggregation is tested.
Laser-induced fluorescence (LIF) is the new technology developing on conventional fluorescent method basis, and laser-induced fluorescence (LIF) is carried out fluorescence excitation with laser as excitaton source.Compare with traditional fluorescence method, laser Induced Fluorescence Technology has better sensitivity and lower detection limit (Zare, R.N., My life with LIF:a personal account of developing laser-induced fluorescence.Annual Review of Analytical Chemistry, 2012.5:p.1-14.).
As shown in Figure 1, conventional laser induced fluorescence system comprises laser instrument, catoptron, focus lamp, sample chamber, optical filter, fiber optic collimator mirror, optical fiber and spectrometer to conventional light path structure in laser Induced Fluorescence Technology.According to different needs, the optical module that laser-induced fluorescence (LIF) system is used can be adjusted to some extent, and light channel structure also can change to some extent, but basic structure is consistent substantially.The laser line focus mirror sending from laser instrument focuses on, arrive sample, sample is subject to producing fluorescence after laser excitation.Fluorescence filters unnecessary laser by optical filter, avoids high-intensity laser signal that the reception of fluorescence signal is produced and disturbed.Then fluorescence focuses on and is coupled into optical fiber through fiber optic collimator mirror, transfers to spectrometer.
This quasi-tradition laser-induced fluorescence (LIF) system has an apparent shortcoming, the fluorescence that the sample being stimulated produces is launched to all directions, and the gathering-device of current used laser-induced fluorescence (LIF) system can only be caught to the fluorescence of a few directions transmitting, cannot catch most of fluorescence to other direction transmitting, capacity gauge and the utilization factor of fluorescence signal are lower, the fluorescence signal intensity obtaining is lower, and then has limited the sensitivity of fluoroscopic examination.
Summary of the invention
The object of the invention is to overcome the shortcoming of above-mentioned conventional laser induced fluorescence system, provide a kind of novel signal to strengthen laser-induced fluorescence (LIF) system, improve capacity gauge and the utilization factor of fluorescence signal, thereby realize signal, strengthen, and then improve the sensitivity of fluoroscopic examination.
Technical scheme of the present invention is: signal strengthens laser-induced fluorescence (LIF) system, comprise laser excitation light path and fluorescence reception light path, described laser excitation light path comprises laser instrument, laser condensing lens, and described fluorescence reception light path comprises sample chamber, parabolic mirror, focus lamp, fiber optic collimator mirror, optical fiber, the spectrometer connecting successively; Parabolic mirror has the axial hole for sample chamber turnover along axis direction, also has the hole of passing through for laser beam on the parabola of parabolic mirror, and described hole is perpendicular to parabolic axis, and paraboloidal focus is on the extended line in hole; The focus of parabolic mirror is positioned on the focus point of laser condensing lens, and the sample in sample chamber is positioned in the focus of parabolic mirror, and the laser signal that laser instrument sends gathers on the sample of sample chamber after laser condensing lens.Sample is excited at parabolic reflector mirror foci place, and the fluorescence sending forms parallel beam outgoing through parabolic mirror reflects, and line focus mirror, fiber optic collimator mirror focus on and be coupled into optical fiber, then through Optical Fiber Transmission to spectrometer.
Further, described fluorescence reception light path also comprises optical filter, and optical filter is between focus lamp and fiber optic collimator mirror.
Further, described parabolic mirror, focus lamp, optical filter, fiber optic collimator mirror are on same axis.
Further, described laser excitation light path also comprises catoptron, and catoptron is between laser instrument and laser condensing lens.
Preferably, the laser beam angle at 45 ° that described catoptron and laser instrument send.
Preferably, described sample chamber consists of quartz ampoule.
The present invention can greatly improve the capacity gauge of fluorescence signal and utilization factor, strengthens fluorescence signal, thereby improves the sensitivity of fluoroscopic examination, simple in structure, compact, is convenient to build.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of conventional laser induced fluorescence system.
Fig. 2 is structural representation of the present invention.
Fig. 3 is the position view in two holes on parabolic mirror.
Fig. 4 is for being used respectively conventional laser induced fluorescence system and the present invention to detect resulting spectrogram.
In figure: 1-laser instrument, 2-catoptron, 3-laser condensing lens, 4-sample chamber, 5-optical filter, 6-fiber optic collimator mirror, 7-optical fiber, 8-spectrometer, 9-parabolic mirror, 10-focus lamp, 11-hole, 12-axial hole, 13-focus.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described, but should not limit the scope of the invention with this.
As shown in Figure 2,3, signal of the present invention strengthens laser-induced fluorescence (LIF) system, comprise laser excitation light path and fluorescence reception light path, described laser excitation light path comprises laser instrument 1, laser condensing lens 3, and described fluorescence reception light path comprises sample chamber 4, parabolic mirror 9, focus lamp 10, fiber optic collimator mirror 6, optical fiber 7, the spectrometer 8 connecting successively; Parabolic mirror 9 has the axial hole 12 for sample chamber 4 turnover along axis direction, also has the hole 11 of passing through for laser beam on the parabola of parabolic mirror 9, and described hole 11 is perpendicular to parabolic axis, and paraboloidal focus 13 is on the extended line in hole 11; The focus 13 of parabolic mirror is positioned on the focus point of laser condensing lens, and the sample in sample chamber 4 is positioned in the focus 13 of parabolic mirror, and like this, the laser signal that laser instrument 1 sends gathers on the sample of sample chamber 4 after laser condensing lens 3.
Further, described fluorescence reception light path also comprises optical filter 5, and optical filter 5 is between focus lamp 10 and fiber optic collimator mirror 6, and the laser that optical filter 5 filterings are unnecessary, avoids high-intensity laser signal to receive to produce to fluorescence signal and disturb.
Further, described parabolic mirror 9, focus lamp 10, optical filter 5, fiber optic collimator mirror 6 are on same axis, to keep the optimum performance of optical system.
Further, described laser excitation light path also comprises catoptron 2, and catoptron 2 is between laser instrument 1 and laser condensing lens 3, and catoptron 2 can change the direction of laser beam, is conducive to the general layout of various parts, makes the more compact structure of system.
Preferably, the laser beam angle at 45 ° that described catoptron 2 and laser instrument 1 send.
Preferably, described sample chamber 4 consists of quartz ampoule, and quartzy good optical property can guarantee that the fluorescence that send laser and sample sample chamber all has very high transmitance.
Focal position and the sample position of laser overlap, simultaneously, this position is the focus 13 of parabolic mirror just also, therefore, the fluorescence sending after sample is excited will, from the focus 13 of parabolic mirror 9 to surrounding transmitting, can be regarded the sample being stimulated as a light source that is positioned at parabolic reflector mirror foci 13.According to the principle of parabolic mirror, fluorescence can become parallel beam and penetrate after parabolic mirror reflects.
Focus lamp 10, fiber optic collimator mirror 6 form a fluorescence focus lamp group, make fluorescence finally can focus on coupled into optical fibres 7.
During work, laser signal is from laser instrument 1 sends, after catoptron 2 reflections, laser condensing lens 3 passes the hole 11 on parabolic mirror 9 after laser signal is assembled, focus on the sample of the sample chamber that is arranged in parabolic reflector mirror foci 13 positions, sample is subject to sending fluorescence after laser excitation, fluorescence becomes parallel beam and penetrates after parabolic mirror 9 reflections, parallel beam line focus mirror 10 is assembled again, light beam is by the unnecessary laser of optical filter 5 filterings, final beam focuses on and is coupled into optical fiber 7 through fiber optic collimator mirror 6, through optical fiber 7, transfer to spectrometer 8 again.
Rhodamine 6G is a kind of common fluorescence probe, is widely used in the fields such as cell biology, environmental chemistry, pharmacology.In order to verify detection performance of the present invention, use respectively the present invention and conventional laser induced fluorescence system to test the rhodamine 6G ethanolic solution (10ng/ml) of same concentration, in test, two cover systems are used same spectrometer (in test, the setting parameter of spectrometer is also in full accord) and same laser instrument, sample chamber 4 also adopts quartz ampoule (the internal diameter 1.2mm of same size, external diameter 2.0mm), the optical element that other two systems are all used is all also same model.The maximum excitation wavelength of rhodamine 6G is near 525nm, maximum emission wavelength (can change to some extent according to actual conditions maximum excitation wavelength and maximum emission wavelength) near 560nm, for realizing optimum detection effect, laser instrument 1 is used the diode pumped solid state laser of 527nm, and the optical filter in fluorescence reception light path is used 550nm long wave pass filter.Test result as shown in Figure 4.From test result, can find, in the test of the fluorescent material rhodamine 6G ethanolic solution to same concentrations, the resulting signal intensity of the present invention obtains far above conventional laser induced fluorescence system.Proved that the present invention compares conventional laser induced fluorescence system and has higher fluorescence signal capacity gauge and fluorescence signal utilization factor, thereby made the present invention under equal conditions can obtain stronger fluorescence signal, and then improved the sensitivity of fluoroscopic examination.
Compare conventional laser induced fluorescence system, feature of the present invention is: introduced a parabolic mirror and matched with focus lamp, optical filter, fiber optic collimator mirror thereafter, improved greatly the capacity gauge of fluorescence signal and utilization factor, thereby increased the sensitivity that fluorescence signal intensity has improved fluoroscopic examination; Installing simple compactness is convenient to build.
Obviously, above embodiment is only made for the present invention giving an example, and the not restriction to embodiment of the present invention.To those of ordinary skill in the art, can also make other changes in different forms on the basis of the above description.Here exhaustive without also giving all embodiments.And these belong to apparent variation that spirit of the present invention amplified out or change still among protection scope of the present invention.
Claims (6)
1. signal strengthens laser-induced fluorescence (LIF) system, it is characterized in that: comprise laser excitation light path and fluorescence reception light path, described laser excitation light path comprises laser instrument (1), laser condensing lens (3), and described fluorescence reception light path comprises sample chamber (4), parabolic mirror (9), focus lamp (10), fiber optic collimator mirror (6), optical fiber (7), the spectrometer (8) connecting successively; Parabolic mirror (9) has the axial hole (12) for sample chamber (4) turnover along axis direction, on the parabola of parabolic mirror (9), also has the hole (11) of passing through for laser beam, described hole (11) is perpendicular to parabolic axis, and paraboloidal focus (13) is on the extended line of hole (11); The focus (13) of parabolic mirror (9) is positioned on the focus point of laser condensing lens (3), sample in sample chamber (4) is positioned in the focus (13) of parabolic mirror, and the laser signal that laser instrument (1) sends gathers on the sample of sample chamber (4) after laser condensing lens (3).
2. signal according to claim 1 strengthens laser-induced fluorescence (LIF) system, it is characterized in that: described fluorescence reception light path also comprises optical filter (5), and optical filter (5) is positioned between focus lamp (10) and fiber optic collimator mirror (6).
3. signal according to claim 2 strengthens laser-induced fluorescence (LIF) system, it is characterized in that: described parabolic mirror (9), focus lamp (10), optical filter (5), fiber optic collimator mirror (6) are on same axis.
4. signal according to claim 1 strengthens laser-induced fluorescence (LIF) system, it is characterized in that: described laser excitation light path also comprises catoptron (2), and catoptron (2) is positioned between laser instrument (1) and laser condensing lens (3).
5. signal according to claim 4 strengthens laser-induced fluorescence (LIF) system, it is characterized in that: the laser beam angle at 45 ° that described catoptron (2) and laser instrument (1) send.
6. signal according to claim 1 strengthens laser-induced fluorescence (LIF) system, it is characterized in that: described sample chamber (4) consist of quartz ampoule.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323873A (en) * | 2016-10-11 | 2017-01-11 | 桂林电子科技大学 | Transmitting/reflecting-combined fluorescent multiplication cuvette |
| CN111896511A (en) * | 2020-08-05 | 2020-11-06 | 中国科学技术大学 | Efficient fluorescence collection device and method for solid state spinning |
| CN112014418A (en) * | 2020-08-13 | 2020-12-01 | 北京大学 | Off-axis reflecting mirror assembly and method for collecting and coupling electron beam excited fluorescence |
| CN116990281A (en) * | 2023-09-27 | 2023-11-03 | 中国科学院合肥物质科学研究院 | Cavity-enhanced Raman detection device with high collection efficiency |
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| CN1567559A (en) * | 2003-07-08 | 2005-01-19 | 浙江大学 | An orthogonal light path type fluorescent detection device for microchip analysis |
| CN1605856A (en) * | 2004-11-18 | 2005-04-13 | 上海交通大学 | Laser fluorescence correlation spectrum unimolecular analyzer |
| CN1963470A (en) * | 2006-11-16 | 2007-05-16 | 天津大学 | Method for detecting parallel light-excited solid fluorescence by vertical optical fibre |
| CN103940709A (en) * | 2014-05-06 | 2014-07-23 | 南京中科神光科技有限公司 | Real-time microbial particle counter |
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2014
- 2014-09-12 CN CN201410464884.6A patent/CN104198452A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040009185A (en) * | 2002-07-22 | 2004-01-31 | 주식회사 옵트론-텍 | Apparatus for detecting fluorescent light and manufacturing method thereof |
| CN1567559A (en) * | 2003-07-08 | 2005-01-19 | 浙江大学 | An orthogonal light path type fluorescent detection device for microchip analysis |
| CN1605856A (en) * | 2004-11-18 | 2005-04-13 | 上海交通大学 | Laser fluorescence correlation spectrum unimolecular analyzer |
| CN1963470A (en) * | 2006-11-16 | 2007-05-16 | 天津大学 | Method for detecting parallel light-excited solid fluorescence by vertical optical fibre |
| CN103940709A (en) * | 2014-05-06 | 2014-07-23 | 南京中科神光科技有限公司 | Real-time microbial particle counter |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323873A (en) * | 2016-10-11 | 2017-01-11 | 桂林电子科技大学 | Transmitting/reflecting-combined fluorescent multiplication cuvette |
| CN111896511A (en) * | 2020-08-05 | 2020-11-06 | 中国科学技术大学 | Efficient fluorescence collection device and method for solid state spinning |
| CN111896511B (en) * | 2020-08-05 | 2022-05-17 | 中国科学技术大学 | Efficient fluorescence collection device and method for solid state spinning |
| CN112014418A (en) * | 2020-08-13 | 2020-12-01 | 北京大学 | Off-axis reflecting mirror assembly and method for collecting and coupling electron beam excited fluorescence |
| CN116990281A (en) * | 2023-09-27 | 2023-11-03 | 中国科学院合肥物质科学研究院 | Cavity-enhanced Raman detection device with high collection efficiency |
| CN116990281B (en) * | 2023-09-27 | 2024-04-26 | 中国科学院合肥物质科学研究院 | Cavity-enhanced Raman detection device with high collection efficiency |
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