CN107535277A - A kind of breeding method of mango detoxic seedling - Google Patents
A kind of breeding method of mango detoxic seedling Download PDFInfo
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- CN107535277A CN107535277A CN201710999475.XA CN201710999475A CN107535277A CN 107535277 A CN107535277 A CN 107535277A CN 201710999475 A CN201710999475 A CN 201710999475A CN 107535277 A CN107535277 A CN 107535277A
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Abstract
A kind of breeding method of mango detoxic seedling.Specifically include:Initially set up Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden, then Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden that original female parent material enters are selected, nursery stock is carried out continuing to inspect virosis infection conditions by random samples by Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden, to maternal plant garden and shows that the mango seedling in garden confirms that disease-free viral disease accelerates breeding nursery stock after infecting.The present invention is by the moral character of multi-level observation nursery stock, just carries out amount reproduction, it is ensured that nursery stock is pure virus-free, also ensures that mango quality is superior and yield is lifted steadily.By implementing the present invention, it is more beneficial for high-quality mango nursery stock batch production, scale, intensive breeds production.
Description
Technical field
The invention belongs to plant seedling to breed new technical field, and in particular to a kind of breeding method of mango detoxic seedling.
Technical background
Mango is to be distributed one of most commonly used fruit tree in the world, originates in India and Malaysia, and India's cultivation history at most, produces
Amount is most, accounts for the 80% of world wide production.Taiwan, Guangdong, Guangxi, Hainan and the south Fujian in China, south of Yunnan, the southeast and
There is mango plantation in the west and south.
The Guangdong in China, Guangxi, the weather of south Fujian, optimum ectogenesis mango seedling.And Guangxi is yield highest
Province, annual production is more than 3.2 hundred million plants/year.
Traditional mango seedling-wood breeding method is:
(1)Seed nucleus is processed:The more loose seed nucleus of selection, cools after shining extra dry white wine, is cut with specialty strand and carefully pushes shell aside, takes out core
Grain, taken out after soaking 3-5 minutes with insecticide, so young shoot can be avoided to be gnawed by ant worm, on shady and cool, ventilation, the ground of lucifuge
Side;Karyosome is buried with the rough sand of moistening.
(2)Kind nuclear transfer:After germinateing(About 10 days), gently dig and open the grains of sand, the healthy and strong karyosome of young shoot is transplanted to farmland
In.Applied fertilizer after 30 days once, never apply it is too more, otherwise can burn seedling.Later herbicide pesticide be can not ignore.
(3)Grafting:After 4 months, young crops there are about 50 centimeters of height, can implement grafting.Budling must choose healthy and strong bar branch, grafting
Time is generally the 4-9 months.After about 3 months, graft union starts slowly to merge, even if mango seedling by breed improvement cultivate into
Work(.
Because mango is tropical fruit (tree), continuous cropping is easily infected by virus, then reduces yield and quality.The maternal plant of mango
Once with virus, virus can also be carried by breeding the seedling come.
The content of the invention
The purpose of the present invention is exactly overcome the deficiencies in the prior art and provides a kind of cultivation side of brand-new mango detoxic seedling
Method.Specifically include:Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden are initially set up, then selectes original maternal material
Expect Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, the displaying garden entered, nursery stock is passed through into Introduction Observation garden, experiment garden, original
Kind garden, maternal plant garden, displaying garden carry out continuing to inspect virosis infection conditions by random samples, to maternal plant garden and show that the mango seedling confirmation in garden is disease-free
Accelerate breeding nursery stock after viral disease infection.
The present invention is achieved through the following technical solutions:
A kind of breeding method of mango detoxic seedling, it is in technical scheme:Initially set up Introduction Observation garden, experiment garden, original seed farm,
Maternal plant garden, displaying garden, then select the Introduction Observation garden of original female parent material entrance, test garden, original seed farm, maternal plant garden, displaying
Garden, nursery stock is carried out continuing to inspect virosis infection feelings by random samples by Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden
Condition, to maternal plant garden and show that the mango seedling in garden confirms that disease-free viral disease accelerates breeding nursery stock after infecting.
Described original female parent material selectes its method and process is as follows:
(1)Improved seeds are introduced:Make original elite stand using the pure individual plant of the seed selection individual plant or kind of improved seeds, can also be from
Outside area or overseas introduce good strains of seeds as original female parent material;
(2)Identification of virus diseases:Can be straight except the kind material introduced from the virus-free breeding storehouse of national fruit nursery stock drug rehabilitation center
Tap into Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, outside displaying garden, remaining original female parent material is both needed to pass through disease first
Viral disease identifies, identified to prove virus-free kind material, Introduction Observation garden of being allowed for access, experiment garden, original seed farm, maternal plant garden,
Displaying garden accelerates breeding for adopting explant;
(3)Mango seedling detoxification:Detoxification is carried out using stem-apex Meristem culture detoxicity method, then by detoxification and breeding out
Mango seedling enter Introduction Observation garden, original seed farm, maternal plant garden.
Described carries out continuing to inspect disease by random samples by nursery stock by Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden
The process and method of viral disease infection conditions be:By the virus-free kind after viruses indentification or by the kind of detoxification first plant in
Introduction Observation garden carries out Horticultural Characters observation identification, screening, preferably plants afterwards and carries out experiment in cultivation in experiment garden, identified nontoxic
Fine individual plant and its 3 plants of offspring be colonized in original seed farm, maternal plant garden is colonized within 20 plants, more than 100 plants are colonized in displaying garden,
The Horticultural Characters of stock tree are periodically identified every year and continue to inspect virosis infection conditions by random samples 1~2 time, confirm disease-free viral disease infection
Start using collection breeding, it is annual to observe Horticultural Characters 1 time to showing garden plant, and a situation arises 2 times for investigation disease.
The method of described acceleration breeding is:After the disease-free viral disease infection of mango seedling confirmation to maternal plant garden and displaying garden,
Start to gather its branch and accelerate to breed nursery stock by way of cuttage or grafting.
The present invention both had the following advantages:
1st, mango detoxic seedling method and measure proposed by the invention is unique, simple production process, easily implements.
2nd, according to China's mango virosis, a situation arises, by implementing the present invention, establishes Introduction Observation garden, original seed
Garden, strengthen collection, introduce new breeds viewing test, progress kind deposit;Maternal plant garden is established, chooses stock tree, strengthens descriptive literature
The virus-free maternal plant of kind is cultivated, and ensures that the explant kind of collection is pure, virus-free infection, to ensure that nursery material does not carry
Virus;Establish breeding seedling propagation garden, there is provided excellent rearing condition, to ensure that the nursery stock bred is virus-free and healthy and strong;Establish
Variety test garden, strengthen screening varieties, its gardening is observed in the part Progeny plants progress field planting displaying to same individual plant
Shape;Variety display garden is established, fully shows breeding advantage, accelerates improved variety popularization.
3rd, the kind newly introduced first is planted carries out Horticultural Characters observation identification, screening in Introduction Observation garden, preferably plants afterwards
Experiment in cultivation is carried out in experiment garden.3 plants of identified nontoxic fine individual plant and its offspring, are colonized in original seed farm, and 20 plants are colonized in
Maternal plant garden, more than 100 plants are colonized in displaying garden.The Horticultural Characters of stock tree are periodically identified every year and continue to inspect virosis infection by random samples
Situation 1-2 times, confirm that disease-free viral disease infection just starts using collection, accelerate breeding nursery stock.To showing garden plant, garden is observed every year
Skill character 1 time, a situation arises 2 times for investigation disease, it is ensured that nursery stock is pure nontoxic;
4th, the present invention just carries out amount reproduction, it is ensured that nursery stock is pure virus-free, also by the moral character of multi-level observation nursery stock
Ensure that mango quality is superior and yield is lifted steadily.
5th, by implementing the present invention, high-quality mango nursery stock batch production, scale is more beneficial for, intensive breeds production.
Embodiment
The method of the present invention is further illustrated with reference to embodiment.
A kind of breeding method of mango detoxic seedling, it is in technical scheme:Initially set up Introduction Observation garden, experiment garden, original
Kind of garden, maternal plant garden, displaying garden, then select Introduction Observation garden, experiment garden, original seed farm, maternal plant garden that original female parent material enters,
Displaying garden, nursery stock is carried out continuing to inspect virosis infection by random samples by Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden
Situation, to maternal plant garden and show that the mango seedling in garden confirms that disease-free viral disease accelerates breeding nursery stock after infecting.
Embodiment is as follows:
1st, original female parent material is selected:
(1)Improved seeds are introduced:Make original elite stand using the pure individual plant of the seed selection individual plant or kind of improved seeds, can also be from
Outside area or overseas introduce good strains of seeds as original female parent material;
(2)Identification of virus diseases:Can be straight except the kind material introduced from the virus-free breeding storehouse of national fruit nursery stock drug rehabilitation center
Tap into Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, outside displaying garden, remaining original female parent material is both needed to pass through disease first
Viral disease identifies, identified to prove virus-free kind material, Introduction Observation garden of being allowed for access, experiment garden, original seed farm, maternal plant garden,
Displaying garden accelerates breeding for adopting explant;
(3)Mango seedling detoxification:Detoxification is carried out using stem-apex Meristem culture detoxicity method, then by detoxification and breeding out
Mango seedling enter Introduction Observation garden, original seed farm, maternal plant garden.
2nd, first planted in Introduction Observation garden carry out garden by the virus-free kind after viruses indentification or by the kind of detoxification
The identification of skill character observation, screening, preferably plant and carry out experiment in cultivation, identified nontoxic fine individual plant and thereafter in experiment garden afterwards
In 3 plants of generation, is colonized in original seed farm, and maternal plant garden is colonized within 20 plants, and more than 100 plants are colonized in displaying garden, and annual periodically identification is maternal
The Horticultural Characters of tree and continue to inspect virosis infection conditions by random samples 1~2 time, it is numerous using collection to confirm that disease-free viral disease infection just starts
Grow, it is annual to observe Horticultural Characters 1 time to showing garden plant, and a situation arises 2 times for investigation disease.
3rd, after the disease-free viral disease infection of mango seedling confirmation to maternal plant garden and displaying garden, just start to gather its branch and pass through skewer
Insert or the mode of grafting accelerates to breed nursery stock.
Claims (1)
- A kind of 1. breeding method of mango detoxic seedling, it is characterised in that:Initially set up Introduction Observation garden, experiment garden, original seed farm, mother This garden, displaying garden, Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden that original female parent material enters then are selected, Nursery stock is carried out continuing to inspect virosis infection conditions by random samples by Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden, it is right Maternal plant garden and the mango seedling in displaying garden accelerate breeding nursery stock after confirming disease-free viral disease infection;Described original female parent material selectes its method and process is as follows:(1)Improved seeds are introduced:Make original elite stand using the pure individual plant of the seed selection individual plant or kind of improved seeds, can also be from Outside area or overseas introduce good strains of seeds as original female parent material;(2)Identification of virus diseases:Can be straight except the kind material introduced from the virus-free breeding storehouse of national fruit nursery stock drug rehabilitation center Tap into Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, outside displaying garden, remaining original female parent material is both needed to pass through disease first Viral disease identifies, identified to prove virus-free kind material, Introduction Observation garden of being allowed for access, experiment garden, original seed farm, maternal plant garden, Displaying garden accelerates breeding for adopting explant;(3)Mango seedling detoxification:Detoxification is carried out using stem-apex Meristem culture detoxicity method, then by detoxification and breeding out Mango seedling enter Introduction Observation garden, original seed farm, maternal plant garden;Described carries out continuing to inspect virosis by random samples by nursery stock by Introduction Observation garden, experiment garden, original seed farm, maternal plant garden, displaying garden The process and method of infection conditions be:First planted by the virus-free kind after viruses indentification or by the kind of detoxification in introducing a fine variety Observation garden carries out Horticultural Characters observation identification, screening, preferably plants afterwards and carries out experiment in cultivation in experiment garden, identified nontoxic excellent 3 plants of good individual plant and its offspring are colonized in original seed farm, and maternal plant garden is colonized within 20 plants, and more than 100 plants are colonized in displaying garden, every year Periodically identify the Horticultural Characters of stock tree and continue to inspect virosis infection conditions by random samples 1~2 time, confirm that disease-free viral disease infection just starts Bred using collection, it is annual to observe Horticultural Characters 1 time to showing garden plant, and a situation arises 2 times for investigation disease;The method of described acceleration breeding is:After the disease-free viral disease infection of mango seedling confirmation to maternal plant garden and displaying garden, just start Gather its branch and accelerate to breed nursery stock by way of cuttage or grafting.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108934508A (en) * | 2018-09-18 | 2018-12-07 | 广西田东泰如鲜品农副产品配送有限公司 | A kind of method of mango cuttage and seedling culture |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105010065A (en) * | 2015-06-26 | 2015-11-04 | 田东县八闽农业综合开发有限公司 | Cultivation method of virus-free seedlings of mangos |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN105010065A (en) * | 2015-06-26 | 2015-11-04 | 田东县八闽农业综合开发有限公司 | Cultivation method of virus-free seedlings of mangos |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108934508A (en) * | 2018-09-18 | 2018-12-07 | 广西田东泰如鲜品农副产品配送有限公司 | A kind of method of mango cuttage and seedling culture |
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Application publication date: 20180105 |