CN105010065A - Cultivation method of virus-free seedlings of mangos - Google Patents
Cultivation method of virus-free seedlings of mangos Download PDFInfo
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- CN105010065A CN105010065A CN201510363135.9A CN201510363135A CN105010065A CN 105010065 A CN105010065 A CN 105010065A CN 201510363135 A CN201510363135 A CN 201510363135A CN 105010065 A CN105010065 A CN 105010065A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- Developmental Biology & Embryology (AREA)
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Abstract
Provided is a cultivation method of virus-free seedlings of mangos.The cultivation method concretely comprises following steps of: firstly, setting up an introduction observation garden, an experimental garden, a foundation stock field, a female plant garden and an exhibition garden; secondarily, selecting original female plant material to enter the introduction observation garden, the experimental garden, the foundation stock field, the female plant garden and the exhibition garden; thirdly, continuously spot-checking infection conditions of viral diseases of nursery stocks by means of the observation garden, the experimental garden, the foundation stock field, the female plant garden and the exhibition garden; and lastly, confirming that no mango in the female plant garden and the exhibition garden is infected with viral diseases and then accelerating propagation of nursery stocks. The cultivation method of virus-free seedlings of mangos has following beneficial effects: through multi-level observation of characteristics of nursery stocks, mass propagation can be carried out in order to ensure that nursery stocks are pure and virus-free and also to make sure that quality and yield of mangos are steadily improved; and by application of the cultivation method, high-quality nursery stocks of mangos can be favorably reproduced in a factory-like, large-scale and intensive mode.
Description
Technical field
The invention belongs to plant seedling breeding new technical field, be specifically related to a kind of breeding method of mango detoxic seedling.
Technical background
Mango is one of the fruit tree the most widely that distributes in the world, originates in India and Malaysia, and at most, output is maximum, accounts for 80% of world wide production for India's cultivation history.Taiwan of China, Guangdong, Guangxi, Hainan and south Fujian, south of Yunnan, the southeast and the west and south have mango to plant.
The weather of Guangdong of China, Guangxi, south Fujian, optimum ectogenesis mango seedling.And Guangxi is the province that output is the highest, annual production is more than 3.2 hundred million strains/year.
Traditional mango seedling-wood breeding method is:
(1) plant core processing: choose comparatively loose kind core, cool after shining extra dry white wine, cut by specialty strand and carefully push shell aside, take out karyosome, take out after soaking 3-5 minute with insecticide, young shoot can be avoided like this to be gnawed by ant worm, in the place of shady and cool, ventilation, lucifuge; Karyosome is buried with moistening rough sand.
(2) plant nucleus transplantation: after germinateing (about 10 days), the grains of sand left by plane gently, are transplanted in farmland by karyosome healthy and strong for young shoot.Apply fertilizer after 30 days once, must guard against use too many, otherwise seedling of burning.Later herbicide pesticide be can not ignore.
(3) grafting: after 4 months, young crops about has 50 centimeters high, can implement grafting.Budling must choose healthy and strong bar branch, and grafting time is generally the 4-9 month.After about 3 months, grafting mouth starts slowly to merge, even if cultivated successfully through the mango seedling of breed improvement.
Because mango is tropical fruit (tree), continuous cropping is very easily infected by virus, then reduces yield and quality.The maternal plant of mango once with virus, the seedling of breeding out also can with virus.
Summary of the invention
Object of the present invention is exactly the breeding method overcoming the deficiencies in the prior art and provide brand-new a kind of mango detoxic seedling.Specifically comprise: first set up Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, then selected original female parent material enter Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, nursery stock is carried out continuation sampling observation virus disease infection conditions by Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, the mango seedling in maternal plant garden and displaying garden is confirmed that anosis viral disease infects rear accelerates multiplication nursery stock.
The present invention is achieved through the following technical solutions:
A kind of breeding method of mango detoxic seedling, be in technical scheme: first set up Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, then selected original female parent material enter Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, nursery stock is carried out continuation sampling observation virus disease infection conditions by Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, the mango seedling in maternal plant garden and displaying garden is confirmed that anosis viral disease infects rear accelerates multiplication nursery stock.
Described original female parent material select its method and process as follows:
(1) improved seeds are introduced: the application seed selection individual plant of improved seeds or the pure individual plant of kind do original elite stand, can also introduce good strains of seeds as original female parent material from district or overseas;
(2) Identification of virus diseases: except the kind material introduced from the virus-free breeding storehouse of national fruit nursery stock drug rehabilitation center can directly enter except Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, all the other original female parent material all need first to pass through Identification of virus diseases, through the kind material that assay certificate is virus-free, Introduction Observation garden of being allowed for access, test garden, original seed farm, maternal plant garden, displaying garden are for adopting explant accelerates multiplication;
(3) mango seedling detoxification: adopt stem-apex Meristem culture detoxicity method to carry out detoxification, then through detoxification and the mango seedling of breeding out enters Introduction Observation garden, original seed farm, maternal plant garden.
Described passes through Introduction Observation garden by nursery stock, test garden, original seed farm, maternal plant garden, the process of sampling observation virus disease infection conditions is carried out continuing in displaying garden and method is: by kind virus-free after viruses indentification or first planted by the kind of detoxification and carry out Horticultural Characters in Introduction Observation garden and observe qualification, screening, plant preferably and carry out experiment in cultivation in test garden, through identifying that nontoxic fine individual plant and offspring 3 strain thereof are colonizated in original seed farm, maternal plant garden is colonizated within 20 strains, more than 100 strains be colonizated in displaying garden, virus disease infection conditions 1 ~ 2 time is inspected in Horticultural Characters and the continuation of annual regularly qualification stock tree by random samples, confirm that anosis viral disease infects and just start application collection breeding, to displaying garden plant, annual observation Horticultural Characters 1 time, and a situation arises 2 times to investigate disease.
The method of described accelerates multiplication is: to maternal plant garden and after showing that the mango seedling in garden confirms that anosis viral disease infects, and just starts to gather its branch and by the mode accelerates multiplication nursery stock of cuttage or grafting.
The present invention both had the following advantages:
1, mango detoxic seedling method and measure proposed by the invention is unique, and production technology simply, is easily implemented.
2, according to China's mango virus disease, a situation arises, by implementing the present invention, sets up Introduction Observation garden, original seed farm, strengthens collection, introduce new breeds viewing test, carries out kind deposit; Set up maternal plant garden, choose stock tree, the virus-free maternal plant strengthening recommended variety is cultivated, and ensures that the explant kind gathered is pure, and virus-free infection, to guarantee that nursery material does not carry virus; Set up breeding seedling propagation garden, excellent rearing condition is provided, to ensure that the nursery stock bred is virus-free and healthy and strong; Set up variety trial garden, strengthen screening varieties, field planting is carried out to the part Progeny plants of same individual plant and shows its Horticultural Characters of observation; Set up Variety display garden, fully show breeding advantage, accelerate improved variety popularization.
3, the new kind introduced first is planted and is carried out Horticultural Characters observation qualification, screening in Introduction Observation garden, plants and carry out experiment in cultivation in test garden preferably.Through identifying nontoxic fine individual plant and offspring 3 strain thereof, be colonizated in original seed farm, 20 strains are colonizated in maternal plant garden, more than 100 strains are colonizated in displaying garden.Virus disease infection conditions 1-2 time is inspected in Horticultural Characters and the continuation of annual regularly qualification stock tree by random samples, confirms that anosis viral disease infects and just starts application collection, accelerates multiplication nursery stock.To displaying garden plant, annual observation Horticultural Characters 1 time, a situation arises 2 times for investigation disease, guarantees that nursery stock is pure nontoxic;
4, the present invention is by the moral character of multi-level observation nursery stock, just carries out amount reproduction, ensure that nursery stock is pure virus-free, also guarantees that the superior and output of mango quality promotes steadily.
5, by implementing the present invention, being more conducive to the batch production of high-quality mango nursery stock, scale, intensive breeding production.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
A kind of breeding method of mango detoxic seedling, be in technical scheme: first set up Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, then selected original female parent material enter Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, nursery stock is carried out continuation sampling observation virus disease infection conditions by Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, the mango seedling in maternal plant garden and displaying garden is confirmed that anosis viral disease infects rear accelerates multiplication nursery stock.
Embodiment is as follows:
1, selected original female parent material:
(1) improved seeds are introduced: the application seed selection individual plant of improved seeds or the pure individual plant of kind do original elite stand, can also introduce good strains of seeds as original female parent material from district or overseas;
(2) Identification of virus diseases: except the kind material introduced from the virus-free breeding storehouse of national fruit nursery stock drug rehabilitation center can directly enter except Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, all the other original female parent material all need first to pass through Identification of virus diseases, through the kind material that assay certificate is virus-free, Introduction Observation garden of being allowed for access, test garden, original seed farm, maternal plant garden, displaying garden are for adopting explant accelerates multiplication;
(3) mango seedling detoxification: adopt stem-apex Meristem culture detoxicity method to carry out detoxification, then through detoxification and the mango seedling of breeding out enters Introduction Observation garden, original seed farm, maternal plant garden.
2, by kind virus-free after viruses indentification or first planted by the kind of detoxification and to carry out Horticultural Characters in Introduction Observation garden and observe qualification, screening, plant preferably and carry out experiment in cultivation in test garden, through identifying that nontoxic fine individual plant and offspring 3 strain thereof are colonizated in original seed farm, maternal plant garden is colonizated within 20 strains, more than 100 strains be colonizated in displaying garden, virus disease infection conditions 1 ~ 2 time is inspected in Horticultural Characters and the continuation of annual regularly qualification stock tree by random samples, confirm that anosis viral disease infects and just start application collection breeding, to displaying garden plant, annual observation Horticultural Characters 1 time, and a situation arises 2 times to investigate disease.
3, to maternal plant garden and after showing that the mango seedling in garden confirms that anosis viral disease infects, just start to gather its branch and by the mode accelerates multiplication nursery stock of cuttage or grafting.
Claims (1)
1. the breeding method of a mango detoxic seedling, it is characterized in that: first set up Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, then selected original female parent material enter Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, nursery stock is carried out continuation sampling observation virus disease infection conditions by Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, the mango seedling in maternal plant garden and displaying garden is confirmed that anosis viral disease infects rear accelerates multiplication nursery stock;
Described original female parent material select its method and process as follows:
(1) improved seeds are introduced: the application seed selection individual plant of improved seeds or the pure individual plant of kind do original elite stand, can also introduce good strains of seeds as original female parent material from district or overseas;
(2) Identification of virus diseases: except the kind material introduced from the virus-free breeding storehouse of national fruit nursery stock drug rehabilitation center can directly enter except Introduction Observation garden, test garden, original seed farm, maternal plant garden, displaying garden, all the other original female parent material all need first to pass through Identification of virus diseases, through the kind material that assay certificate is virus-free, Introduction Observation garden of being allowed for access, test garden, original seed farm, maternal plant garden, displaying garden are for adopting explant accelerates multiplication;
(3) mango seedling detoxification: adopt stem-apex Meristem culture detoxicity method to carry out detoxification, then through detoxification and the mango seedling of breeding out enters Introduction Observation garden, original seed farm, maternal plant garden;
Described passes through Introduction Observation garden by nursery stock, test garden, original seed farm, maternal plant garden, the process of sampling observation virus disease infection conditions is carried out continuing in displaying garden and method is: by kind virus-free after viruses indentification or first planted by the kind of detoxification and carry out Horticultural Characters in Introduction Observation garden and observe qualification, screening, plant preferably and carry out experiment in cultivation in test garden, through identifying that nontoxic fine individual plant and offspring 3 strain thereof are colonizated in original seed farm, maternal plant garden is colonizated within 20 strains, more than 100 strains be colonizated in displaying garden, virus disease infection conditions 1 ~ 2 time is inspected in Horticultural Characters and the continuation of annual regularly qualification stock tree by random samples, confirm that anosis viral disease infects and just start application collection breeding, to displaying garden plant, annual observation Horticultural Characters 1 time, and a situation arises 2 times to investigate disease,
The method of described accelerates multiplication is: to maternal plant garden and after showing that the mango seedling in garden confirms that anosis viral disease infects, and just starts to gather its branch and by the mode accelerates multiplication nursery stock of cuttage or grafting.
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| CN201510363135.9A CN105010065A (en) | 2015-06-26 | 2015-06-26 | Cultivation method of virus-free seedlings of mangos |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340668A (en) * | 2015-11-12 | 2016-02-24 | 平果县果燕缘农业开发有限公司 | Cultivation method of virus-free seedlings for sand sugar oranges |
| CN105475136A (en) * | 2015-12-11 | 2016-04-13 | 百色学院 | Culture method for mango stem segment |
| CN107396798A (en) * | 2017-08-04 | 2017-11-28 | 博白县富山水果种植专业合作社 | A kind of implantation methods of citrus |
| CN107535277A (en) * | 2017-10-24 | 2018-01-05 | 田东县八闽农业综合开发有限公司 | A kind of breeding method of mango detoxic seedling |
| CN107691079A (en) * | 2017-10-31 | 2018-02-16 | 博白县富山水果种植专业合作社 | A kind of implantation methods of mango |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835316A (en) * | 2012-09-26 | 2012-12-26 | 中法合营王朝葡萄酿酒有限公司 | Method for removing grape virus disease and rapidly propagating seedling for cabernet gernischet grape variety |
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2015
- 2015-06-26 CN CN201510363135.9A patent/CN105010065A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835316A (en) * | 2012-09-26 | 2012-12-26 | 中法合营王朝葡萄酿酒有限公司 | Method for removing grape virus disease and rapidly propagating seedling for cabernet gernischet grape variety |
Non-Patent Citations (4)
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| 丁伟平等: "湖南省柑桔无病毒良种繁育中心"三圃三园"建设实践与探讨", 《中国南方果树》 * |
| 周常勇等: "柑桔良种无病毒苗木繁育体系建设", 《广西园艺》 * |
| 李亚芸等: "苹果无病毒苗木繁育技术规程", 《山西果树》 * |
| 赵学源等: "柑桔无病毒良种库的建立", 《中国南方果树》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340668A (en) * | 2015-11-12 | 2016-02-24 | 平果县果燕缘农业开发有限公司 | Cultivation method of virus-free seedlings for sand sugar oranges |
| CN105475136A (en) * | 2015-12-11 | 2016-04-13 | 百色学院 | Culture method for mango stem segment |
| CN107396798A (en) * | 2017-08-04 | 2017-11-28 | 博白县富山水果种植专业合作社 | A kind of implantation methods of citrus |
| CN107535277A (en) * | 2017-10-24 | 2018-01-05 | 田东县八闽农业综合开发有限公司 | A kind of breeding method of mango detoxic seedling |
| CN107691079A (en) * | 2017-10-31 | 2018-02-16 | 博白县富山水果种植专业合作社 | A kind of implantation methods of mango |
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