CN107519432A - One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water - Google Patents
One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water Download PDFInfo
- Publication number
- CN107519432A CN107519432A CN201710595783.6A CN201710595783A CN107519432A CN 107519432 A CN107519432 A CN 107519432A CN 201710595783 A CN201710595783 A CN 201710595783A CN 107519432 A CN107519432 A CN 107519432A
- Authority
- CN
- China
- Prior art keywords
- parts
- saprolegniasis
- water
- salvelinus malma
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/90—Smilacaceae (Catbrier family), e.g. greenbrier or sarsaparilla
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/287—Chrysanthemum, e.g. daisy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/739—Sanguisorba (burnet)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Prevent and treat to add body and application thereof in Salvelinus malma saprolegniasis water the invention discloses one kind, the composition and its parts by weight of the addition body are：18 23 parts of Chinese gall, 6 12 parts of garden burnet, 8 12 parts of radix glycyrrhizae, 10 15 parts of prepared rhizome of rehmannia, 8 12 parts of folium isatidis, 58 parts of sweet wormwood, 34 parts of smilax, 23 parts of kuh-seng, 58 parts of the bighead atractylodes rhizome, 57 parts of chrysanthemum indicum.Have the beneficial effect that：Present invention addition body it is each into belonging to renewable resource, can complete biodegradable, it is pollution-free to the breeding water body of Salvelinus malma the problems such as in the absence of medicament residue and drug resistance；It is reasonable to add body compatibility, there is good effect, the safe and advantage that has no toxic side effect to Salvelinus malma saprolegniasis, be not likely to produce the resistance to the action of a drug；The generation of Salvelinus malma saprolegniasis can be prevented and treated, reduces the case fatality rate because of saprolegniasis, reduces cultivation risk.
Description
Technical field
The present invention relates to body is added in a kind of water, prevent and treat in Salvelinus malma saprolegniasis water more particularly to one kind
Add body and purposes.
Background technology
Salvelinus malma is a kind of fish of salmonidae torgoch category, and common people are frequently referred to " spending inner lamb ", Salvelinus malma
Delicious meat is delicate, rich in trophisms such as abundant protein, aliphatic acid, amino acid, therefore deep is liked by vast consumer
Love.In the last few years, the configuration of the Salvelinus malma of artificial farming and feed makes the success of Salvelinus malma artificial domestication, but with
It is increasing cultivation scale, Salvelinus malma is also faced with many problems during cultivation, such as due to spring, summer
Temperature it is higher, Salvelinus malma body surface white, hairy material and with having showing for red and swollen bleeding on body is occurred
As early stage is not easy to observe, uncontrollable until that can use soon out, such a disease is commonly referred to as saprolegniasis, is
A kind of fungi autoeciousness disease, such a fungi are Saprolegnia, Achyla etc..The method of in general treatment saprolegniasis is all to use
The medicine of chemical classes, such as soaked with benzoic acid, sorbic acid etc., or fish are subjected to bathing with light salt brine, but with such a
Injury of the method to fish is king-sized, fish is also resisted many bad reactions in the case of inherently sick, to fish
Injury it is too big.Therefore, a kind of traditional Chinese medicine ingredients that can be added in water are invented and to fish in itself without any injury
Additive is extremely necessary that natural, drug effect is notable because Chinese herbal medicine picks up from, not only economic but also safety, while among Chinese medicine also
Containing the bioactive substance such as abundant mineral matter, vitamin, protein and alkaloid, black alcohols, glucoside, these materials are all
It is the secondary metabolite of plant, can be as the compound combinatorial libraries of a variety of target spots, so biological vivo environment can be improved, carried
The immunocompetence and nutrition condition of high biology.The plurality of advantages of Chinese herbal medicine water quality additive is directed to, invention one kind can be pre-
What anti-and treatment Salvelinus malma saprolegniasis water body additive was extremely necessary.
Prior art such as Authorization Notice No. is the B of CN 103041332 Chinese invention patent, discloses treatment fish and water mildew
Pure Chinese medicine composition and preparation method thereof, the Chinese medicine composition mixes extraction by following components and formed in parts by weight：Five times
Sub- 25-45 parts, calamus 25-35 parts, polygonum flaccidum 25-45 parts, ginger 15-25 parts, tobacco leaf 25-35 parts, salt 15-35 parts, kuh-seng 20-
40 parts, wrinkled giant hyssop 15-35 parts.The Chinese medicine composition reasonable recipe, prepare simply, pure Chinese medicine composition, treat both principal and secondary aspect of disease, curative effect is very
Significantly, it is clinical to prove there is special efficacy to treatment fish and water mildew.But each raw material of Chinese medicine composition has simply carried out simple decoction,
Middle the effective elements of the medicine fails fully to discharge, and the utilization rate of Chinese medicine is low.
The content of the invention
High it is an object of the invention to provide a kind of effective ingredient content, Chinese herbal medicine utilization rate is high, to the flower red point of lamb
Salmon saprolegniasis has prevention effect obvious, good effect, the safe and prevention that has no toxic side effect and treats Salvelinus malma water
Body and purposes are added in mildew water.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is：One kind is prevented and to treat colored lamb red
Body is added in point salmon saprolegniasis water, composition and its parts by weight are：It is Chinese gall 18-23 parts, garden burnet 6-12 parts, radix glycyrrhizae 8-12 parts, ripe
Glutinous rehmannia 10-15 parts, folium isatidis 8-12 parts, sweet wormwood 5-8 parts, smilax 3-4 parts, kuh-seng 2-3 parts, bighead atractylodes rhizome 5-8 parts, chrysanthemum indicum 5-7
Part.Above-mentioned addition body respectively into belonging to renewable resource, can complete biodegradable, to the breeding water body of Salvelinus malma without dirt
Dye, compatibility is carried out using China's tradition Chinese veterinarian's theory, is helped digestion with QI invigorating, based on detoxification and sores, there is antibacterial, antiviral work
With having good effect, the safe and advantage that has no toxic side effect to Salvelinus malma saprolegniasis.
A kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water, specifically includes following steps：
By formula measure tobacco leaf, Chinese gall, frutus cnidii, wilsonii, the Chinese bulbul, the Radix Astragali, dent look for, folium isatidis, chrysanthemum indicum, perfume (or spice)
Ru, kuh-seng, 40-70 mesh is crushed to, produces medicinal powder, standby, suitable medicinal powder particle diameter can increase the contact surface of mixed powder and water
Product, it is possible to increase leaching rates, shorten extraction time, reduce energy consumption, save processing cost；
It is 1 to press solid-liquid ratio to medicinal powder:7-10 adds water, then adds active peptides and enzyme, the addition of active peptides is medicinal powder
The 0.012-0.016% of weight, the addition of enzyme are the 1.8-2.5% of raw material coarse granule weight, are well mixed, and regulation pH is 5-6,
Hydrolyze 60-80min at 50-60 DEG C, enzyme deactivation, centrifugation, stay supernatant, it is standby, can leniently cell dispersion wall by enzyme reaction
And the composition such as cellulose in cytoplasm, pectic substance, the dense construction of cell membrane is destroyed, causes cell membrane and cytoplasm knot
Structure produces the changes such as partial relaxation expansion, collapse, reduces the mass transfer such as cell membrane and cytoplasm barrier to active ingredient from intracellular
The resistance to mass tranfer spread to Extraction medium, so as to be advantageous to the dissolution of active ingredient；
By supernatant concentration to pasty state, 100-120 mesh is crushed to after freeze-drying, produces addition body, the addition body is curative for effect,
Water mo(u)ld fast and effeciently can be thoroughly killed, the generation of Salvelinus malma saprolegniasis is prevented and treated, reduces the case fatality rate because of saprolegniasis, carry
The survival rate of high Salvelinus malma, aquaculture cost is reduced, and addition body is more to water mold germ application point, is not likely to produce the resistance to the action of a drug,
The advantages of with safe, residual less, avoid the residual of western medicine antibiotics, great industrialization prospect.
Preferably, the amino acid sequence of active peptides is HSHSYSRCYCRCLRCRVLHPGCVNCSR, the active peptides
The coarse grained cell membrane of Chinese herbal medicine is acted on, the ion channel of cross-film is formed on film, destroys the integrality of cell membrane, is made thin
The active drug composition quick release of intracellular comes out, and does not influence the activity of active ingredient.
Preferably, enzyme is the mixture of cellulase, pectase and papain, and its mass ratio is 1:0.4-0.6:0.4-
0.6, the cell membrane main component of plant cell is cellulose and pectin, can using cellulase and pectinase treatment Chinese herbal medicine
The cell membrane of plant is destroyed, changes the permeability of cell membrane, improves the dissolution rate of effective component of chinese medicine, reduces the broken of active ingredient
Bad rate, and papain can be hydrolyzed into protein small-molecular peptides, drug effect can be greatly improved while except foreigh protein removing.
A kind of purposes for preventing and treating to add body in Salvelinus malma saprolegniasis water, it is characterised in that：Described addition
The purposes of body is：Addition body is made into concentration to be sprayed in breeding pool for 1-2mg/L medicaments.The red point of addition body preventing and treating flower lamb
The generation of salmon saprolegniasis, the case fatality rate because of saprolegniasis is reduced, improve the survival rate of Salvelinus malma, improve breeding income, reduced
Cultivation risk；The addition body has the function that clearing heat and detoxicating, sterilization antibacterial, without adding antibiotic again, can improve the red point of colored lamb
The resistance of salmon, it is safe to use, the problems such as in the absence of medicament residue and drug resistance, there are wide market prospects.
Compared with prior art, the advantage of the invention is that：1）Present invention addition body it is each into belonging to renewable resource,
Can complete biodegradable, it is pollution-free to the breeding water body of Salvelinus malma the problems such as in the absence of medicament residue and drug resistance；2）
It is reasonable to add body compatibility, there is antibacterial, antiviral, have to Salvelinus malma saprolegniasis good effect, it is safe and
The advantage having no toxic side effect, it is more to water mold germ application point, it is not likely to produce the resistance to the action of a drug；3）Extraction rate of the present invention is fast, the time is short,
The recovery rate of effective ingredient is greatly improved, can effectively protect medium-height grass the effective elements of the medicine, the purity of active ingredient is high, peace
Entirely, save, be pollution-free；4）The generation of Salvelinus malma saprolegniasis can be prevented and treated, reduces the case fatality rate because of saprolegniasis, improves flower lamb
The survival rate of torgoch, breeding income is improved, reduce cultivation risk.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
One kind prevents and treated to add body in Salvelinus malma saprolegniasis water, and composition and its parts by weight are：20 parts of Chinese gall, garden burnet
9 parts, 10 parts of radix glycyrrhizae, 12 parts of prepared rhizome of rehmannia, 10 parts of folium isatidis, 7 parts of sweet wormwood, 3.5 parts of smilax, 2.5 parts of kuh-seng, 7 parts of the bighead atractylodes rhizome, mother chrysanthemum
Spend 6 parts.
A kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water, specifically includes following steps：
1）By formula measure tobacco leaf, Chinese gall, frutus cnidii, wilsonii, the Chinese bulbul, the Radix Astragali, dent look for, folium isatidis, chrysanthemum indicum, perfume (or spice)
Ru, kuh-seng, through mechanical crushing to 40-70 mesh, medicinal powder is produced, standby, suitable medicinal powder particle diameter can increase mixed powder and water
Contact area, it is possible to increase leaching rates, shorten extraction time, reduce energy consumption, save processing cost；
2）It is 1 to press solid-liquid ratio to medicinal powder:7-10 adds water, then adds active peptides and enzyme, the addition of active peptides is medicine
The 0.012-0.016% of powder weight, the addition of enzyme are the 1.8-2.5% of raw material coarse granule weight, are well mixed, regulation pH is 5-
6,60-80min is hydrolyzed at 50-60 DEG C, the enzyme deactivation 10min at 100 DEG C, rotating speed is subsequently placed in and is centrifuged for 5000r/min
12min, supernatant is stayed, it is standby, can the leniently cellulose in cell dispersion wall and cytoplasm, pectic substance etc. by enzyme reaction
Composition, the dense construction of cell membrane is destroyed, cause cell membrane and cytoplasm structure to produce partial relaxation expansion, collapse etc. and become
Change, reduce the resistance to mass tranfer that the mass transfer such as cell membrane and cytoplasm barrier spreads to active ingredient from intracellular to Extraction medium, from
And be advantageous to the dissolution of active ingredient, moreover it is possible to remove foreign protein；
3）By supernatant concentration to pasty state, 110 mesh are crushed to after freeze-drying, produce addition body, the addition body is curative for effect, energy
Water mo(u)ld is fast and effeciently thoroughly killed, the generation of Salvelinus malma saprolegniasis is prevented and treated, reduces the case fatality rate because of saprolegniasis, is improved
The survival rate of Salvelinus malma, aquaculture cost is reduced, and addition body is more to water mold germ application point, is not likely to produce the resistance to the action of a drug, has
There is the advantages of safe, residual is few, avoid the residual of western medicine antibiotics, great industrialization prospect.
The amino acid sequence of active peptides is HSHSYSRCYCRCLRCRVLHPGCVNCSR in above-mentioned steps 2, and the activity is more
Peptide acts on the coarse grained cell membrane of Chinese herbal medicine, and the ion channel of cross-film is formed on film, destroys the integrality of cell membrane,
Intracellular active drug composition quick release is come out, and does not influence the activity of active ingredient.
The enzyme of above-mentioned steps 2 is the mixture of cellulase, pectase and papain, and its mass ratio is 1:0.5:0.5, plant
The cell membrane main component of cell is cellulose and pectin, using cellulase and pectinase treatment Chinese herbal medicine, can destroy plant
Cell membrane, change the permeability of cell membrane, improve the dissolution rate of effective component of chinese medicine, reduce the destructive rate of active ingredient, and
Protein can be hydrolyzed into small-molecular peptides by papain, can greatly improve drug effect while except foreigh protein removing.
A kind of purposes for preventing and treating to add body in Salvelinus malma saprolegniasis water, it is characterised in that：Described addition
The purposes of body is：Addition body is made into concentration to be sprayed in breeding pool for 1.5mg/L medicaments.The red point of addition body preventing and treating flower lamb
The generation of salmon saprolegniasis, the case fatality rate because of saprolegniasis is reduced, improve the survival rate of Salvelinus malma, improve breeding income, reduced
Cultivation risk；The addition body has the function that clearing heat and detoxicating, sterilization antibacterial, without adding antibiotic again, can improve the red point of colored lamb
The resistance of salmon, it is safe to use, the problems such as in the absence of medicament residue and drug resistance, there are wide market prospects.
Embodiment 2：
One kind prevents and treated to add body in Salvelinus malma saprolegniasis water, and composition and its parts by weight are：19 parts of Chinese gall, garden burnet
9 parts, 8 parts of radix glycyrrhizae, 11 parts of prepared rhizome of rehmannia, 9 parts of folium isatidis, 5 parts of sweet wormwood, 3 parts of smilax, 3 parts of kuh-seng, 6 parts of the bighead atractylodes rhizome, 7 parts of chrysanthemum indicum.
A kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water, specifically includes following steps：
By formula measure tobacco leaf, Chinese gall, frutus cnidii, wilsonii, the Chinese bulbul, the Radix Astragali, dent look for, folium isatidis, chrysanthemum indicum, perfume (or spice)
Ru, kuh-seng, 40 mesh are crushed to, produce medicinal powder, standby, suitable medicinal powder particle diameter can increase the contact area of mixed powder and water,
Leaching rates can be improved, shorten extraction time, reduce energy consumption, save processing cost；
It is 1 to press solid-liquid ratio to medicinal powder:9 add water, then add active peptides and enzyme, the addition of active peptides is medicinal powder weight
0.013%, the addition of enzyme is the 2% of raw material coarse granule weight, is well mixed, and regulation pH is 5.2, is hydrolyzed at 55 DEG C
65min, enzyme deactivation, centrifugation, supernatant is stayed, standby, fiber that can leniently in cell dispersion wall and cytoplasm by enzyme reaction
The compositions such as element, pectic substance, the dense construction of cell membrane is destroyed, cause cell membrane and cytoplasm structure generation partial relaxation swollen
The changes such as swollen, collapse, reduce what the mass transfer such as cell membrane and cytoplasm barrier spread to active ingredient from intracellular to Extraction medium
Resistance to mass tranfer, so as to be advantageous to the dissolution of active ingredient, moreover it is possible to remove foreign protein；
By supernatant concentration to pasty state, 100 mesh are crushed to after freeze-drying, produce addition body, the addition body is curative for effect, can be fast
Speed effectively thoroughly kills water mo(u)ld, prevents and treats the generation of Salvelinus malma saprolegniasis, reduces the case fatality rate because of saprolegniasis, improves flower
The survival rate of lamb torgoch, aquaculture cost is reduced, and addition body is more to water mold germ application point, is not likely to produce the resistance to the action of a drug, has
The advantages of safe, residual is lacked, avoid the residual of western medicine antibiotics, great industrialization prospect.
The amino acid sequence of above-mentioned active peptides is HSHSYSRCYCRCLRCRVLHPGCVNCSR, the work of the active peptides
For the coarse grained cell membrane of Chinese herbal medicine, the ion channel of cross-film is formed on film, the integrality of cell membrane is destroyed, makes cell
Interior active drug composition quick release comes out, and does not influence the activity of active ingredient.
Above-mentioned enzyme is the mixture of cellulase, pectase and papain, and its mass ratio is 1:0.4:0.6, plant cell
Cell membrane main component be cellulose and pectin, using cellulase and pectinase treatment Chinese herbal medicine, the thin of plant can be destroyed
Cell wall, change the permeability of cell membrane, improve the dissolution rate of effective component of chinese medicine, reduce the destructive rate of active ingredient, and pawpaw
Protein can be hydrolyzed into small-molecular peptides by enzyme, can greatly improve drug effect while except foreigh protein removing.
A kind of purposes for preventing and treating to add body in Salvelinus malma saprolegniasis water, it is characterised in that：Described addition
The purposes of body is：Addition body is made into concentration to be sprayed in breeding pool for 1.3mg/L medicaments.The red point of addition body preventing and treating flower lamb
The generation of salmon saprolegniasis, the case fatality rate because of saprolegniasis is reduced, improve the survival rate of Salvelinus malma, improve breeding income, reduced
Cultivation risk；The addition body has the function that clearing heat and detoxicating, sterilization antibacterial, without adding antibiotic again, can improve the red point of colored lamb
The resistance of salmon, it is safe to use, the problems such as in the absence of medicament residue and drug resistance, there are wide market prospects.
Embodiment 3：
One kind prevents and treated to add body in Salvelinus malma saprolegniasis water, and composition and its parts by weight are：22 parts of Chinese gall, garden burnet
7 parts, 11 parts of radix glycyrrhizae, 10 parts of prepared rhizome of rehmannia, 9 parts of folium isatidis, 7 parts of sweet wormwood, 3.8 parts of smilax, 3 parts of kuh-seng, 6 parts of the bighead atractylodes rhizome, chrysanthemum indicum 7
Part.
It is a kind of prevent and treat in Salvelinus malma saprolegniasis water add body preparation method be：By formula measure tobacco leaf,
Chinese gall, frutus cnidii, wilsonii, the Chinese bulbul, the Radix Astragali, dent are looked for, folium isatidis, chrysanthemum indicum, elscholtiza, kuh-seng, be crushed to 70 mesh and obtain
Medicinal powder, it is 1 that solid-liquid ratio is pressed into medicinal powder:9 add water, then add active peptides and enzyme, the addition of active peptides is medicinal powder
The 0.015% of weight, enzyme are that mass ratio is 1:0.5:0.6 cellulase, pectase and pawpaw enzymatic mixture, the addition of enzyme
For the 2.2% of raw material coarse granule weight, it is well mixed, regulation pH is 6, and 75min, enzyme deactivation, centrifugation, by supernatant are hydrolyzed at 54 DEG C
Liquid is concentrated into pasty state, and 100 mesh are crushed to after freeze-drying, produces addition body.
A kind of purposes for preventing and treating to add body in Salvelinus malma saprolegniasis water, it is characterised in that：Described addition
The purposes of body is：Addition body is made into concentration to be sprayed in breeding pool for 2mg/L medicaments.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Tonghua Teachers College
<120>One kind prevents and treated to add body and application thereof in Salvelinus malma saprolegniasis water
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 27
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ser Tyr Ser Arg Cys Tyr Cys Arg Cys Leu Arg Cys Arg
1 5 10 15
Val Leu His Pro Gly Cys Val Asn Cys Ser Arg
20 25
Claims (10)
1. one kind prevents and treated in Salvelinus malma saprolegniasis water to add body, it is characterised in that：The composition of described addition body
And its parts by weight are：Chinese gall 18-23 parts, garden burnet 6-12 parts, radix glycyrrhizae 8-12 parts, prepared rhizome of rehmannia 10-15 parts, folium isatidis 8-12 parts,
Sweet wormwood 5-8 parts, smilax 3-4 parts, kuh-seng 2-3 parts, bighead atractylodes rhizome 5-8 parts, chrysanthemum indicum 5-7 parts.
2. one kind according to claim 1 prevents and treated in Salvelinus malma saprolegniasis water to add body, it is characterised in that：
The preparation method of described addition body comprises the following steps：
1）By formula measure tobacco leaf, Chinese gall, frutus cnidii, wilsonii, the Chinese bulbul, the Radix Astragali, dent look for, folium isatidis, chrysanthemum indicum, perfume (or spice)
Ru, kuh-seng, crush, it is standby；
2）Add water, active peptides and enzyme by feed liquid to medicinal powder, be well mixed, adjust pH, hydrolysis, enzyme deactivation, centrifugation, stay supernatant,
It is standby；
3）By supernatant concentration to pasty state, crushed after freeze-drying, produce addition body.
3. a kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water according to claim 2,
It is characterized in that：Described step 2 traditional Chinese medicine powder and the solid-liquid ratio of water are 1:7-10.
4. a kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water according to claim 2,
It is characterized in that：In described step 2 addition of active peptides be medicinal powder weight 0.012-0.016%, its amino acid sequence
For HSHSYSRCYCRCLRCRVLHPGCVNCSR.
5. a kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water according to claim 2,
It is characterized in that：The addition of enzyme is the 1.8-2.5% of raw material coarse granule weight in described step 2.
6. a kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water according to claim 2,
It is characterized in that：Enzyme is the mixture of cellulase, pectase and papain in described step 2, and its mass ratio is 1:0.4-
0.6:0.4-0.6。
7. a kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water according to claim 2,
It is characterized in that：It is 5-6 that pH is hydrolyzed in described step 2, and temperature is 50-60 DEG C, time 60-80min.
8. a kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water according to claim 2,
It is characterized in that：Described step 1 Central Plains feed powder is broken to 40-70 mesh.
9. a kind of preparation method for preventing and treating to add body in Salvelinus malma saprolegniasis water according to claim 2,
It is characterized in that：100-120 mesh is crushed to after being freeze-dried in described step 3.
A kind of 10. purposes for preventing and treating to add body in Salvelinus malma saprolegniasis water, it is characterised in that：Described addition body
Purposes be：Addition body is made into concentration to be sprayed in breeding pool for 1-2mg/L medicaments.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710595783.6A CN107519432A (en) | 2017-07-20 | 2017-07-20 | One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710595783.6A CN107519432A (en) | 2017-07-20 | 2017-07-20 | One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107519432A true CN107519432A (en) | 2017-12-29 |
Family
ID=60749063
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710595783.6A Pending CN107519432A (en) | 2017-07-20 | 2017-07-20 | One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107519432A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108096314A (en) * | 2018-02-06 | 2018-06-01 | 成都市农林科学院 | A kind of composition for treating fresh-water fishes saprolegniasis, preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142592A (en) * | 2013-02-01 | 2013-06-12 | 上海海洋大学 | Medicine for preventing fish saprolegniasis and application thereof |
| CN103416337A (en) * | 2013-08-24 | 2013-12-04 | 宁波市大桥生态农业有限公司 | Wild-imitation breeding method for river eel |
| CN106420984A (en) * | 2016-12-18 | 2017-02-22 | 长沙瑞多康生物科技有限公司 | Composition for preventing and treating saprolegniasis of fishes |
| CN106879876A (en) * | 2017-01-22 | 2017-06-23 | 嵊州市派特普科技开发有限公司 | Feed of grass carp with lifting immunity function and preparation method thereof |
-
2017
- 2017-07-20 CN CN201710595783.6A patent/CN107519432A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142592A (en) * | 2013-02-01 | 2013-06-12 | 上海海洋大学 | Medicine for preventing fish saprolegniasis and application thereof |
| CN103416337A (en) * | 2013-08-24 | 2013-12-04 | 宁波市大桥生态农业有限公司 | Wild-imitation breeding method for river eel |
| CN106420984A (en) * | 2016-12-18 | 2017-02-22 | 长沙瑞多康生物科技有限公司 | Composition for preventing and treating saprolegniasis of fishes |
| CN106879876A (en) * | 2017-01-22 | 2017-06-23 | 嵊州市派特普科技开发有限公司 | Feed of grass carp with lifting immunity function and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘富梁等: "酶法在中药提取中的研究进展", 《时珍国医国药》 * |
| 宋晓亮等: "《全国普通高等医学院校护理学类专业"十三五"规划教材 药理学》", 31 August 2016, 中国医药科技出版社 * |
| 宫毓静等: "164种中药乙醇提取物抗真菌作用研究", 《中草药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108096314A (en) * | 2018-02-06 | 2018-06-01 | 成都市农林科学院 | A kind of composition for treating fresh-water fishes saprolegniasis, preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101669651B1 (en) | Composition for red ginseng mask pack, the manufacturing method thereof, and the mask pack manufactured thereby | |
| CN107595893A (en) | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof | |
| CN110604249A (en) | Astaxanthin-containing microcapsule effervescent tablet and preparation method thereof | |
| CN106074450A (en) | The bioactivity protective technique prepared for medical material superfine powder | |
| CN103550402A (en) | Preparation method for fructus lycii extract | |
| CN108671148A (en) | A kind of dendrobium candidum leaf effervescent and preparation method thereof | |
| CN103054939A (en) | Preparation technology of liquorice ultrafine powder granular decoction pieces | |
| CN110368339A (en) | Face mask of traditional Chinese medicine composition and preparation method thereof, application method | |
| CN107519432A (en) | One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water | |
| JPH0358939A (en) | Aging preventive food agent and production thereof | |
| CN105211859A (en) | A kind of agate coffee wheat seeding oral tablet and preparation method thereof | |
| CN107811913A (en) | A kind of application of Chinese medicine composition in oral care product | |
| CN104398426A (en) | Mask for treating common acne | |
| CN109418677A (en) | A kind of compound protein solid beverage that can improve spacefarer's eyesight | |
| CN107114424A (en) | A kind of pepper planting insecticide and preparation method | |
| CN106857692A (en) | Application of the Solidago Canadensis in Pomacea canaliculata is killed | |
| CN106690121A (en) | Trepang, hippophae rhamnoides fruit, cordyceps militaris and selenium preparation and preparation technology thereof | |
| CN109998980A (en) | Chinese medicine white hair treats shampoo powder and preparation method thereof | |
| CN109938335A (en) | A kind of Rhizoma Gastrodiae cynomorium songaricum chewable tablets and preparation method thereof | |
| CN107410764A (en) | A kind of Chinese herbal feed additive for preventing golden trout ich and application thereof | |
| CN114642236A (en) | Chlorella natto tablet candy capable of assisting in lowering blood pressure | |
| CN113040177A (en) | Plant fermentation extract for degrading pesticide residues of crops and preparation method thereof | |
| CN105853338A (en) | Dandruff removal traditional Chinese medicine composition containing tropaeolum majus and used for cosmetics | |
| CN113018404A (en) | Blood stasis constitution health-care solid beverage and preparation method thereof | |
| CN105688146A (en) | Healthcare capsule for improving sleeping quality |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171229 |