CN108096314A - A kind of composition for treating fresh-water fishes saprolegniasis, preparation method and application - Google Patents
A kind of composition for treating fresh-water fishes saprolegniasis, preparation method and application Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
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- A61K2236/10—Preparation or pretreatment of starting material
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Abstract
The invention discloses a kind of composition for treating fresh-water fishes saprolegniasis, preparation method and applications, it is made of the raw material of following parts by weight：Fresh 5 10 parts of Golden Larch Bark, 20 50 parts of the tincture of iodine, 35 parts of kuh-seng, 13 parts of licorice tablet, 38 parts of bioadhesive material, 0.2 0.5 parts of papain, 58 parts of alginates, 0 15 parts of water, by removal of impurities；Ultramicro grinding；Infusion；Mixing；Enzymolysis：Papain is added in, heating stands cooling；Glue：Until it is formed in gelatinous composition.Beneficial effects of the present invention are：Using Golden Larch Bark and the tincture of iodine as raw material; it is equipped with kuh-seng and Radix Glycyrrhizae; improve the therapeutic effect of fresh-water fishes saprolegniasis under breeding scale environment; and by infusion, enzymolysis, glue preparation process; gel combination is obtained, adds drug effect sustained release cycle and the use scope of composition, you can use directly to smear; it can also be watered and embathe, especially suitable for scale aquaculture.
Description
Technical field
The present invention relates to aquatic livestock Prevention Technique field, especially a kind of composition for treating fresh-water fishes saprolegniasis, system
Preparation Method and application.
Background technology
Saprolegniasis（Saprolegniasis）Often infection body surface wounded tissue and dead ovum form covering for canescence such as flocculence
Cover material also known as covers cotton disease or water cotton disease, also known as dermatomycosis or white hair disease, is one of nosomycosis of aquatic fish, causes this disease
Pathogen to having had now been found that more than ten kinds, one of the most common is that water mold and silk floss are mould.The disease is by fungi autoeciousness fish
Body surface causes, mainly the Saprolegnia of Eumycota Mastigomycotina Phycomycetes Saprolegniales Saprolegniaceae and Achyla.Fish is just caught
Any symptom is not seen when sick.When fish length " white hair " is found, mycelia has invaded fish body wound, spreads between musculature
Among gap.With progression of the disease, affected part muscular decay, anorexia is final dead.This disease is interior at all seasons can all to be sent out
It is raw.The generation of saprolegniasis in order to prevent, damage fish body is prevented when should be noted that * makees as far as possible and by parasitic insect bite in addition to, may be used also
To be put into a small amount of salt in water, to inhibit the generation of saprolegniasis.Fish body grows " white hair ", in canescence, mycelia and wound
Cell tissue winding adherency makes tissue necrosis, while sick fish anorexia, last dead.
At present, saprolegniasis is directed to, the method for use substantially has：1st, with salt sodium bicarbonate mixed liquor is added to splash；2nd, hole is used
Sparrow malachite green solution is embathed.Wherein, salt adds the mixed liquor of sodium bicarbonate inconvenient, can not be carried out according to fish body disease condition
Effective treatment in real time, can only achieve the inhibition of certain sterilization and saprolegniasis.In comparison.It is controlled with malachite green
The effect of certain effect can be reached by treating saprolegniasis, but due to the medical instrument have " three cause ", high residue, it is not degradable the defects of, because
And China in 2002 prohibites and malachite green is used in aquaculture.Meanwhile the drug of existing treatment saprolegniasis is most
Based on splashing or embathe, the effect of drug in water is poor, and the drug effect sustained release performance of drug is poor, it is necessary to often more dressing
Object, and be applied in fish body since swing of the fish body can depart from drug, the therapeutic effect of anticipation is not achieved.
Under background based on aquatic products large-scale cultivation, the situation of fresh-water fishes mass-sending saprolegniasis happens occasionally, therefore, either
In order to meet the actual needs of aquiculture disease prevention still for Safety of Aquatic Products, safe efficient prevention is developed
The drug of fresh-water fishes saprolegniasis is all very urgent.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of composition for treating fresh-water fishes saprolegniasis,
Preparation method and application can improve the therapeutic effect of fresh-water fishes saprolegniasis under breeding scale environment, increase the medicine of composition
Effect sustained release cycle and use scope.
The purpose of the present invention is what is be achieved through the following technical solutions：A kind of composition for treating fresh-water fishes saprolegniasis,
It is made of the raw material of following parts by weight：
Fresh Golden Larch Bark 5-10 parts, 20-50 parts of the tincture of iodine, 3-5 parts of kuh-seng, 1-3 parts of licorice tablet, 3-8 parts of bioadhesive material, wood
.2-0.5 parts of melon proteinase-10,5-8 parts of alginates, 0-15 parts of water, wherein, the concentration of the tincture of iodine is 8-10%.
Golden Larch Bark is pinaceae plant golden larch（Pseudolarix kamepferi Gord.）Dry root skin or nearly root tree
Skin.Summer strips, and drying can be used as medicine.Golden Larch Bark contains more ingredient, such as organic acid, ursin, isovanillin, vanilla
Acid, cupreol etc., the Golden Larch Bark 10 kind pathomycetes common to China have certain antibacterial action, therefore in existing record
Document in, be related to the record that Golden Larch Bark uses on saprolegniasis.Most is all simply carried out using Golden Larch Bark as auxiliary material
It uses, and not up to its real medical value.
The bioadhesive material is chitosan, carbomer, xanthans, hyaluronic acid, collagen, polyoxyethylene
One or more of.Due to mucous membrane of animal can secreting mucus, main component is viscous glycoprotein, glycoprotein, lipoid, inorganic
Salt, water etc., and viscous glycoprotein can make mucus have the characteristics such as gluey, cohesion and bonding.The good profit of body tissue mucomembranous surface
Wet bar part makes swellable polymer material generate close contact therewith, the molecule segment insertion space between cells of adhesion material or with
Sticky segment in mucus penetrates mutually, passes through mechanical interlock, covalent bond, electrostatic attraction, Van der Waals force, hydrogen bond, hydrophobic bond
Comprehensive functions are waited, polymer is closely linked with mucous membrane, so as to generate bioadhesion phenomenon, and when can remain considerably long
Between.Therefore, applicant considers to increase the use scope in fish body using bioadhesive material.Bioadhesive material has one
Fixed slow release effect, drug effect can be played for a long time by being attached to the composition of affected area.
The adhesion material is CARBOPOL 971 or 974P.
The alginates are sodium alginate, and sodium alginate can form soft gel on lesion surface, is cured for wound
It closes and preferable moist environment is provided, promote the active ingredient in Golden Larch Bark（Such as organic acid, aldehydes）Accelerate to absorb, wound is promoted to be cured
It closes, alleviates wound pain, meanwhile, further improve the adhesion effect of drug.
The activity of the papain is ten thousand U/mg of 100-300.Papain is a kind of proteolytic enzyme, molecule
It measures as 23406, is made of a kind of single chain polypeptide, containing 212 amino acid residues, be at least present in enzyme there are three amino acid residue
Activated centre position, they are Cys25, His159 and Asp158 respectively, when Cys25 by it is oxidizing or with metal ion knot
During conjunction, the vigor of enzyme is suppressed, and reducing agent cysteine（Or sulphite）Or EDTA can recover the vigor 0 of enzyme.Other six
A cysteine residues form three pairs of disulfide bond, and not in active site.In this application, applicant utilizes Papain
Enzyme temperature is a kind of low specificity proteolytic enzyme, can digest ingredient extra in Golden Larch Bark, further improve therapeutic effect.
The preparation method of the composition of the treatment fresh-water fishes saprolegniasis, it is comprised the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, and for use, Golden Larch Bark selects surfaces externally and internally to be advisable to be russet；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 500-700 mesh
Sieve, obtains subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 36-48h at 25-45 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 2-4h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 50-65 DEG C and keep 1-2h, digest
Process carries out under closed environment, stands cooling, papain is a kind of low specificity proteolytic enzyme, and temperature can influence
Its activity and enzymolysis efficiency can carry out partial hydrolysis at 50-65 DEG C to the active ingredient in Golden Larch Bark（Mild hydrolysis）, obtain
Composition can reach optimal therapeutic effect；
S6, glue：The bioadhesive material, alginates and water for adding in above-mentioned parts by weight uniformly mix, and stand until being formed
Viscosity is in gelatinous composition more than 3000mPas's.
The preparation method of the composition of described treatment fresh-water fishes saprolegniasis, in the ultramicro grinding step, respectively first
Golden Larch Bark, kuh-seng and licorice tablet are crushed to the fine particle of 10-15mm, by fine particle be put into micronizer into
Row ultramicro grinding, until the Golden Larch Bark, kuh-seng and licorice tablet that weigh parts by weight all sieve.
The preparation method of the composition of described treatment fresh-water fishes saprolegniasis is set on the closed lighttight container
There is the temperature display instrument of external, can accurately show heating problems, improve the efficiency of preparation.
The preparation method of the composition of described treatment fresh-water fishes saprolegniasis, the gel group obtained in the glue step
The viscosity for closing object is the viscosity at 25 DEG C.
The composition is in the application in a manner of being applied to lesion surface on prevention fresh-water fishes saprolegniasis.
The combination of Golden Larch Bark and the tincture of iodine only has disclosed record in treatment athlete's foot at present, in treatment fish saprolegniasis
On have no direct record.Active ingredient in the tincture of iodine can sterilize the wound of affected area, based on bioadhesive material
The effect being sustained in water can promote the active ingredient in Golden Larch Bark（Such as organic acid, aldehydes）Accelerate to absorb, improve fish
The therapeutic effect of large-scale cultivation, in tincture of iodine content 8-10%, best results.Kuh-seng and Radix Glycyrrhizae can aid in that wound is promoted to be cured
It closes, is common auxiliary medicinal material in Medicines in Aquaculture.
The beneficial effects of the invention are as follows：Using Golden Larch Bark and the tincture of iodine as raw material, kuh-seng and Radix Glycyrrhizae are equipped with, improves breeding scale
The therapeutic effect of fresh-water fishes saprolegniasis under environment, and by infusion, enzymolysis, glue preparation process, obtain gel combination,
Add drug effect sustained release cycle and the use scope of composition, you can it is used with directly smearing, can also be watered and embathe, it is especially suitable
For scale aquaculture.
Specific embodiment
Technical scheme is described in further detail with reference to embodiment, but protection scope of the present invention is not limited to
In as described below.
Embodiment 1
A kind of composition for treating fresh-water fishes saprolegniasis, it is made of the raw material of following parts by weight：
Fresh 8 parts of Golden Larch Bark, 30 parts of the tincture of iodine, 3 parts of kuh-seng, 2 parts of licorice tablet, 5 parts of bioadhesive material, papain 0.3
Part, 7 parts of alginates, 5 parts of water, wherein, the concentration of the tincture of iodine is 8.5%.
The preparation method of the composition of the treatment fresh-water fishes saprolegniasis, it is comprised the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, for use；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 550 mesh sieves, obtains
To subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 42h at 28 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 2.5h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 55 DEG C and keep 2h, enzymolysis process
It is carried out under closed environment, stands cooling；
S6, glue：The bioadhesive material, alginates and water for adding in above-mentioned parts by weight uniformly mix, and stand until being formed
Viscosity is in gelatinous composition more than 3000mPas's.
Embodiment 2
A kind of composition for treating fresh-water fishes saprolegniasis, it is made of the raw material of following parts by weight：
Fresh 5 parts of Golden Larch Bark, 20 parts of the tincture of iodine, 4 parts of kuh-seng, 1 part of licorice tablet, 3 parts of bioadhesive material, papain 0.2
Part, 6 parts of alginates, 15 parts of water, wherein, the concentration of the tincture of iodine is 8%.
The preparation method of the composition of the treatment fresh-water fishes saprolegniasis, it is comprised the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, for use；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 500 mesh sieves, obtains
To subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 36h at 30 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 2h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 50 DEG C and keep 2h, enzymolysis process
It is carried out under closed environment, stands cooling；
S6, glue：The bioadhesive material, alginates and water for adding in above-mentioned parts by weight uniformly mix, and stand until being formed
Viscosity is in gelatinous composition more than 3000mPas's.
Embodiment 3
A kind of composition for treating fresh-water fishes saprolegniasis, it is made of the raw material of following parts by weight：
Fresh 10 parts of Golden Larch Bark, 40 parts of the tincture of iodine, 5 parts of kuh-seng, 3 parts of licorice tablet, 8 parts of bioadhesive material, papain 0.3
Part, 5 parts of alginates, 5 parts of water, wherein, the concentration of the tincture of iodine is 10%.
The preparation method of the composition of the treatment fresh-water fishes saprolegniasis, it is comprised the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, for use；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 700 mesh sieves, obtains
To subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 48h at 25 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 4h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 65 DEG C and keep 1h, enzymolysis process
It is carried out under closed environment, stands cooling；
S6, glue：The bioadhesive material, alginates and water for adding in above-mentioned parts by weight uniformly mix, and stand until being formed
Viscosity is in gelatinous composition more than 3000mPas's.
Embodiment 4
A kind of composition for treating fresh-water fishes saprolegniasis, it is made of the raw material of following parts by weight：
Fresh 7 parts of Golden Larch Bark, 35 parts of the tincture of iodine, 3.5 parts of kuh-seng, 1.5 parts of licorice tablet, 6 parts of bioadhesive material, papain
0.5 part, 8 parts of alginates, 10 parts of water, wherein, the concentration of the tincture of iodine is 9%.
The preparation method of the composition of the treatment fresh-water fishes saprolegniasis, it is comprised the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, for use；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 600 mesh sieves, obtains
To subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 46h at 45 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 3h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 65 DEG C and keep 1.5h, digested
Cheng Jun is carried out under closed environment, stands cooling；
S6, glue：The bioadhesive material, alginates and water for adding in above-mentioned parts by weight uniformly mix, and stand until being formed
Viscosity is in gelatinous composition more than 3000mPas's.
Embodiment 5
A kind of composition for treating fresh-water fishes saprolegniasis, it is made of the raw material of following parts by weight：
Fresh 6 parts of Golden Larch Bark, 50 parts of the tincture of iodine, 4 parts of kuh-seng, 2 parts of licorice tablet, 5 parts of bioadhesive material, papain 0.4
Part, 7 parts of alginates, wherein, the concentration of the tincture of iodine is 9.5%.
The preparation method of the composition of the treatment fresh-water fishes saprolegniasis, it is comprised the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, for use；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 500 mesh sieves, obtains
To subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 40h at 40 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 3.5h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 62 DEG C and keep 1.5h, digested
Cheng Jun is carried out under closed environment, stands cooling；
S6, glue：The bioadhesive material and alginates for adding in above-mentioned parts by weight uniformly mix, and stand until forming viscosity
What it is more than 3000mPas is in gelatinous composition.
Embodiment 6
A kind of composition for treating fresh-water fishes saprolegniasis, it is made of the raw material of following parts by weight：
Fresh 8 parts of Golden Larch Bark, 20-50 parts of the tincture of iodine, 5 parts of kuh-seng, 3 parts of licorice tablet, 8 parts of bioadhesive material, papain
0.35 part, 8 parts of alginates, 12 parts of water, wherein, the concentration of the tincture of iodine is 9%.
The preparation method of the composition of the treatment fresh-water fishes saprolegniasis, it is comprised the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, for use；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 600 mesh sieves, obtains
To subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 46h at 38 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 4h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 58 DEG C and keep 2h, enzymolysis process
It is carried out under closed environment, stands cooling；
S6, glue：The bioadhesive material, alginates and water for adding in above-mentioned parts by weight uniformly mix, and stand until being formed
Viscosity is in gelatinous composition more than 3000mPas's.
The gel combination that embodiment 1-6 is obtained both can be smeared directly, can also be watered dilution and use.Directly smear
As long as suitable for the extremely serious sick body of saprolegniasis affected area, directly smeared in affected area, compared with the prior art, this Shen
It please only needs to smear 1-2 times, it is easy to operate, and existing operation frequently replaces the mode of liquid medicine mostly using impregnating
To reach therapeutic effect.Large-scale cultivation is directed to simultaneously, obtained gel combination can first be added in warm water by Gel Compositions
Object dissolves, then dilutes（100-150 times）To full pond, standard of preferably splashing 1-1.5g/m3, wherein, 50-65 DEG C of greenhouse temperature
It is advisable.
The above is only the preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form is not to be taken as the exclusion to other embodiment, and available for various other combinations, modification and environment, and can be at this
In the text contemplated scope, it is modified by the technology or knowledge of above-mentioned introduction or association area.And those skilled in the art institute into
Capable modifications and changes do not depart from the spirit and scope of the present invention, then all should be in the protection domain of appended claims of the present invention
It is interior.
Claims (10)
1. a kind of composition for treating fresh-water fishes saprolegniasis, which is characterized in that it is made of the raw material of following parts by weight：
Fresh Golden Larch Bark 5-10 parts, 20-50 parts of the tincture of iodine, 3-5 parts of kuh-seng, 1-3 parts of licorice tablet, 3-8 parts of bioadhesive material, wood
.2-0.5 parts of melon proteinase-10,5-8 parts of alginates, 0-15 parts of water, wherein, the concentration of the tincture of iodine is 8-10%.
A kind of 2. composition for treating fresh-water fishes saprolegniasis as described in claim 1, which is characterized in that the bioadhesion
Property material be chitosan, carbomer, xanthans, hyaluronic acid, collagen, one or more of polyoxyethylene.
A kind of 3. composition for treating fresh-water fishes saprolegniasis as claimed in claim 2, which is characterized in that the adhesiveness material
Expect for CARBOPOL 971 or 974P.
4. a kind of composition for treating fresh-water fishes saprolegniasis as described in claim 1, which is characterized in that the alginates are
Sodium alginate.
A kind of 5. composition for treating fresh-water fishes saprolegniasis as described in claim 1, which is characterized in that the Papain
The activity of enzyme is ten thousand U/mg of 100-300.
6. the preparation method of the composition of the treatment fresh-water fishes saprolegniasis as described in claim 1-5 is any, which is characterized in that it
It comprises the steps of：
S1, removal of impurities：The uniform Golden Larch Bark of surface color, kuh-seng, Radix Glycyrrhizae are selected, weighs Golden Larch Bark, the hardship of above-mentioned parts by weight respectively
Ginseng, licorice tablet are cleaned and remove impurity removing, for use；
S2, ultramicro grinding：Golden Larch Bark, kuh-seng and the licorice tablet obtained to S1 steps carries out ultramicro grinding, crosses 500-700 mesh
Sieve, obtains subtle powder；
S3, infusion：The tincture of iodine of above-mentioned parts by weight is transferred in closed lighttight container, it is subtle then to add in above-mentioned Golden Larch Bark
Powder is heated to infusion 36-48h at 25-45 DEG C, and infusion process carries out under closed environment, stands cooling；
S4, mixing：The subtle powder of kuh-seng and the subtle powder of Radix Glycyrrhizae are added in said vesse, is stirred evenly, stands 2-4h；
S5, enzymolysis：It adds in the papain of above-mentioned parts by weight and stirs evenly, be heated to 50-65 DEG C and keep 1-2h, digest
Process carries out under closed environment, stands cooling；
S6, glue：The bioadhesive material, alginates and water for adding in above-mentioned parts by weight uniformly mix, and stand until being formed
Viscosity is in gelatinous composition more than 3000mPas's.
7. the preparation method of the composition for the treatment of fresh-water fishes saprolegniasis as claimed in claim 6, which is characterized in that described is super
In Crushing of Ultrafine step, Golden Larch Bark, kuh-seng and licorice tablet are first crushed to the fine particle of 10-15mm respectively, by subtle
Grain, which is put into micronizer, carries out ultramicro grinding, until the Golden Larch Bark, kuh-seng and licorice tablet that weigh parts by weight all sieve.
8. the preparation method of the composition for the treatment of fresh-water fishes saprolegniasis as claimed in claim 6, which is characterized in that described is close
It closes on lighttight container and is provided with the temperature display instrument of external.
9. the preparation method of the composition for the treatment of fresh-water fishes saprolegniasis as claimed in claim 6, which is characterized in that the system
The viscosity of the gel combination obtained in glue step is the viscosity at 25 DEG C.
10. composition as described in claim 1 in a manner of being applied to lesion surface prevention fresh-water fishes saprolegniasis on
Using.
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| CN201810119654.4A CN108096314A (en) | 2018-02-06 | 2018-02-06 | A kind of composition for treating fresh-water fishes saprolegniasis, preparation method and application |
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Citations (4)
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| JPS643127A (en) * | 1987-06-23 | 1989-01-06 | Yusuke Shigyo | Method for preventing and treating disease of aquatic organism |
| CN106581207A (en) * | 2017-03-06 | 2017-04-26 | 长沙瑞多康生物科技有限公司 | Composition for preventing and controlling fish Saprolegniasis |
| CN107412200A (en) * | 2017-04-27 | 2017-12-01 | 哈尔滨乾佰纳生物药业有限公司 | A kind of spacetabs type stomatocace film with bioadhesive and preparation method thereof |
| CN107519432A (en) * | 2017-07-20 | 2017-12-29 | 通化师范学院 | One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water |
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2018
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|---|---|---|---|---|
| JPS643127A (en) * | 1987-06-23 | 1989-01-06 | Yusuke Shigyo | Method for preventing and treating disease of aquatic organism |
| CN106581207A (en) * | 2017-03-06 | 2017-04-26 | 长沙瑞多康生物科技有限公司 | Composition for preventing and controlling fish Saprolegniasis |
| CN107412200A (en) * | 2017-04-27 | 2017-12-01 | 哈尔滨乾佰纳生物药业有限公司 | A kind of spacetabs type stomatocace film with bioadhesive and preparation method thereof |
| CN107519432A (en) * | 2017-07-20 | 2017-12-29 | 通化师范学院 | One kind prevents and treated to add body and purposes in Salvelinus malma saprolegniasis water |
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| 倪达书: "《鱼类水霉病的防治研究》", 28 February 1982, 农业出版社 * |
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