CN107518005A - A kind of sex immature phase Bactrocera tau bait formulation and preparation method thereof - Google Patents
A kind of sex immature phase Bactrocera tau bait formulation and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses a kind of sex immature phase Bactrocera tau bait formulation, mixed by volumes below part component：100 parts of albumen bait formulation, 23 parts of the pungent lipoprotein solution of acetic acid, 23 parts of benzaldehyde solution, 23 parts of 3 methyl, 1 butanol solution；The pungent lipoprotein solution of acetic acid is using paraffin oil as solvent, and the pungent fat of acetic acid is that solute makes the pungent lipoprotein solution of acetic acid that volumetric concentration is 20% 30%；Benzaldehyde solution is using paraffin oil as solvent, and benzaldehyde is that solute makes the benzaldehyde solution that volumetric concentration is 20% 30%；The butanol solution of 3 methyl 1 is using paraffin oil as solvent, and the butanol of 3 methyl 1 is that solute makes the butanol solution of 3 methyl 1 that volumetric concentration is 20% 30%.The beneficial effect that this hair has is：Sex immature phase Bactrocera tau bait formulation can play preferable attracting action to female or male sex immature phase Bactrocera tau simultaneously, can be killed before Bactrocera tau does not damage, and not have chemical residual, environmentally safe, simple to operate, quick.The invention also discloses a kind of preparation method of sex immature phase Bactrocera tau bait formulation.
Description
Technical field
The present invention relates to insect derivant and preparation method thereof, more particularly to a kind of sex immature phase Bactrocera tau bait formulation and
Its preparation method.
Background technology
For a long time, China is mainly gyplure trapping male worm, Agro-chemicals control and agriculture for trypetid preventing control method
The several methods such as industry preventing and treating, but prevention effect is undesirable, and because gyplure only traps and kills male worm, and what is damaged is female adult, and
Bactrocera tau and melonfly have the habit of multiple mating, eliminate part male worm and are had little significance to reducing population quantity, it is important to
Eliminate female adult；Chemicals to intrusion fruit inside larva it is invalid, strong to flight performance adult effect it is undesirable, to piercing
The mature larva and pupa pupated in soil have little effect, and excessive use chemical pesticide can produce a series of side effect；
Agricultural measures are cumbersome, time-consuming.
In consideration of it, have developed the albumen bait formulation for having attracting action to male and female trypetid again, and albumen bait formulation to environment without
Pollution, hazard residue will not be produced to fruit.Therefore albumen bait formulation becomes indispensable in prevention and control Bactrocera tau and melonfly
A ring.But albumen bait formulation is general to the attractant effect of Bactrocera tau and melonfly at present.In order to improve drawing for albumen bait formulation
Effect is lured, prior art mainly has methyl eugenol, vinegar and brown sugar, phoxim, malathion etc. to albumen bait formulation additive, this
Although a little materials have certain effect to trypetid prevention and control tool, because the composition of compositing formula is single or proportioning is less reasonable, or
Sexual maturing period and the egg production peak time of melonfly and Bactrocera tau are directed to, and the Bactrocera tau and melon to the two periods are real
Injury of the fly to agricultural product has resulted in, and therefore, protection effect is often undesirable when reality uses.Addition phoxim, horse in addition
While drawing the introducing of the organophosphorus pesticides such as sulphur phosphorus to improve albumen bait formulation service efficiency, this kind of agricultural chemicals also result in the dirt of environment
Dye.
In consideration of it, inventor furthers investigate to above mentioned problem, there is this case generation then.
The content of the invention
In view of the deficiencies in the prior art, it is an object of the invention to provide it is a kind of can be simultaneously to the female of sex immature phase
Or male Bactrocera tau plays preferable attractant effect, is killed before Bactrocera tau does not damage, and without chemical residual and
The green sex immature phase Bactrocera tau bait formulation of environmental pollution.
Another object of the present invention is to provide it is a kind of can be simultaneously to the female or male Bactrocera tau of sex immature phase
Preferable attractant effect is played, is killed before Bactrocera tau does not damage, and without chemical residual and the green of environmental pollution
The preparation method of the sex immature phase Bactrocera tau bait formulation of environmental protection.
To achieve these goals, the present invention adopts the following technical scheme that：
A kind of sex immature phase Bactrocera tau bait formulation, is mainly mixed by volumes below part component：
The above-mentioned pungent lipoprotein solution of acetic acid is using paraffin oil as solvent, and the pungent fat of acetic acid is that to make volumetric concentration be 20%- to solute
The 30% pungent lipoprotein solution of acetic acid；
Above-mentioned benzaldehyde solution is using paraffin oil as solvent, and benzaldehyde is that to make volumetric concentration be 20%-30% to solute
Benzaldehyde solution；
Above-mentioned 3- methyl-1-butanols solution is using paraffin oil as solvent, and 3- methyl-1-butanols are that to make volume dense for solute
Spend the 3- methyl-1-butanol solution for 20%-30%.
A kind of sex immature phase Bactrocera tau bait formulation, is mixed by volumes below part component：
The above-mentioned pungent lipoprotein solution of acetic acid is using paraffin oil as solvent, and the pungent fat of acetic acid is that to make volumetric concentration be 20% to solute
The pungent lipoprotein solution of acetic acid；
Above-mentioned benzaldehyde solution is that benzaldehyde is that solute makes the benzene that volumetric concentration is 25% using paraffin oil as solvent
Formalin；
Above-mentioned 3- methyl-1-butanols solution is using paraffin oil as solvent, and 3- methyl-1-butanols are that to make volume dense for solute
Spend the 3- methyl-1-butanol solution for 25%.
A kind of preparation method of sex immature phase Bactrocera tau bait formulation, is made by the steps and forms：(1) additive
Prepare：
A, prepared by the pungent lipoprotein solution of acetic acid, and using paraffin oil as solvent, the pungent fat of acetic acid is that to make volumetric concentration be 20%- to solute
The 30% pungent lipoprotein solution of acetic acid,
B, prepared by benzaldehyde solution, and using paraffin oil as solvent, benzaldehyde is that to make volumetric concentration be 20%-30% to solute
Benzaldehyde solution；
C, prepared by 3- methyl-1-butanols solution, and using paraffin oil as solvent, 3- methyl-1-butanols are that solute makes volume
Concentration is 20%-30% 3- methyl-1-butanol solution；
(2) mix：By the pungent lipoprotein solution of acetic acid, benzaldehyde solution and the 3- methyl-1-butanols solution and albumen bait of step (1)
Agent is mixed by eddy mixer and together, obtains described Bactrocera tau bait formulation；The pungent lipoprotein solution of acetic acid, benzaldehyde are molten
Liquid, 3- methyl-1-butanols solution and albumen bait formulation are mixed by volumes below part：
The volumetric concentration of the above-mentioned pungent lipoprotein solution of acetic acid is 20%, and the volumetric concentration of above-mentioned benzaldehyde solution is 25%, above-mentioned
The volumetric concentration of 3- methyl-1-butanol solution is 25%, the parts by volume of the above-mentioned pungent lipoprotein solution of acetic acid, the body of above-mentioned benzaldehyde solution
The parts by volume of product part and above-mentioned 3- methyl-1-butanols solution is 2.5 parts.
After technical scheme, sex immature phase Bactrocera tau bait formulation can simultaneously to female or male not
Maturity period Bactrocera tau plays preferable attracting action, can be killed before Bactrocera tau does not damage, and residual without chemistry
Stay, it is environmentally safe, it is simple to operate, it is quick.
Brief description of the drawings
Attractant effects of the Fig. 1 for different albumen bait formulation in outdoor measure to sex immature phase Bactrocera tau；
Attractant effects of the Fig. 2 for different albumen bait formulation in outdoor measure to sex immature phase melonfly.
Embodiment
With reference to specific embodiment, the present invention is described further.
A kind of sex immature phase Bactrocera tau bait formulation of the present invention, is mixed by volumes below part component：
The above-mentioned pungent lipoprotein solution of acetic acid is using paraffin oil as solvent, and the pungent fat of acetic acid is that to make volumetric concentration be 20%- to solute
The 30% pungent lipoprotein solution of acetic acid；
Above-mentioned benzaldehyde solution is using paraffin oil as solvent, and benzaldehyde is that to make volumetric concentration be 20%-30% to solute
Benzaldehyde solution；
Above-mentioned 3- methyl-1-butanols solution is using paraffin oil as solvent, and 3- methyl-1-butanols are that to make volume dense for solute
Spend the 3- methyl-1-butanol solution for 20%-30%.
The sex immature phase Bactrocera tau bait formulation of the present invention, by playing strong Electroantennogram response volatility to Bactrocera tau
Material associated proteins bait formulation is set about to study sex immature phase Bactrocera tau albumen pollution-free, that attractant effect is excellent.The present invention
Beneficial effect be：Because the pungent fat of acetic acid has denseer similar flores aurantii and Jasmine fragrance, benzaldehyde has cherry and nut perfume,
It is accustomed to according to the feeding of trypetid, such smell can produce attractant effect to trypetid, and another 3 kinds of additives can be simultaneously to Bactrocera tau
Produce Electroantennogram response and increase attractant effect；Further, benzaldehyde, 3- methyl-1-butanols and being mutually dissolved property of octyl acetate
Preferably, side effect will not be produced to respective attractant effect after mixing, paraffin oil colorless and odorless, fugitive constituent are few, dissolve in albumen
In bait formulation, while benzaldehyde, 3- methyl-1-butanols and octyl acetate are soluble in paraffin oil, are that solvent can make from paraffin oil
Benzaldehyde, 3- methyl-1-butanols and octyl acetate mix more uniform with albumen bait formulation, avoid mixed liquor from being layered so that mixed
The attractant effect that liquid plays albumen bait formulation and additive simultaneously is closed, the experiment proved that the sex immature phase Bactrocera tau bait of this formula
Agent can be killed before Bactrocera tau does not damage, because additive is naturally present in plant, and the amount pole added
Small volatilization i.e. basic after use finishes, therefore will not cause chemical residual, environmentally safe, in addition sex immature phase South Asia
The only simple mixing of the preparation of trypetid bait formulation, it is simple to operate, it is quick.
Experiment for many years through the present inventor researches and develops to obtain：When the addition using paraffin oil as solvent added in albumen bait formulation
Agent solution is excessive, and after additive solution mixes with albumen bait formulation, the inhibitory action that the volatile ingredient in mixed liquor is subject to is big, meeting
The attractant effect of mixed liquor is reduced, it is very few as the additive solution of solvent using paraffin oil when being added in albumen bait formulation, it is corresponding
The content of additive also reduce, can also reduce the attractant effect of mixed liquor.
The sex immature phase Bactrocera tau bait formulation of the present invention, preferably：The volumetric concentration of the pungent lipoprotein solution of acetic acid is 20%,
Parts by volume is 2.5 parts, and the volumetric concentration of benzaldehyde solution is 25%, and parts by volume is 2.5 parts, 3- methyl-1-butanol solution
Volumetric concentration is 25%, and parts by volume is 2.5 parts.
The sex immature phase Bactrocera tau bait formulation of the present invention, is made by the steps and forms：
(1) prepared by additive：
A, prepared by the pungent lipoprotein solution of acetic acid, and using paraffin oil as solvent, the pungent fat of acetic acid is that to make volumetric concentration be 20%- to solute
The 30% pungent lipoprotein solution of acetic acid, preferably：The volumetric concentration of the pungent lipoprotein solution of acetic acid is 20%,
B, prepared by benzaldehyde solution, and using paraffin oil as solvent, benzaldehyde is that to make volumetric concentration be 20%-30% to solute
Benzaldehyde solution, preferably：The volumetric concentration of benzaldehyde solution is 25%；
C, prepared by 3- methyl-1-butanols solution, and using paraffin oil as solvent, 3- methyl-1-butanols are that solute makes volume
Concentration is 20%-30% 3- methyl-1-butanol solution, preferably：The volumetric concentration of 3- methyl-1-butanol solution is
25%；
(2) mix：By the pungent lipoprotein solution of acetic acid, benzaldehyde solution and the 3- methyl-1-butanols solution and albumen bait of step (1)
Agent is mixed by eddy mixer and together, obtains described Bactrocera tau bait formulation；The pungent lipoprotein solution of acetic acid, benzaldehyde are molten
Liquid, 3- methyl-1-butanols solution and albumen bait formulation are mixed by volumes below part：
Wherein, optimal volume part of the pungent lipoprotein solution of acetic acid is 2.5 parts, and optimal volume part of benzaldehyde solution is 2.5 parts, 3-
Optimal volume part of methyl-1-butanol solution is 2.5 parts.
The sex immature phase Bactrocera tau bait formulation of the present invention determines its attractant effect, test side using indoor bioassay
Formula is as follows：It is (female that sex immature phase Bactrocera tau 50 is put into each test-cage：Male=1:1), cage top portion diagonal is placed
One sponge containing clear water, to keep the humidity in cage.Per cage, center places one and traps bottle, the bait formulation sample that internal frame prepares
Product.Length of testing speech is 6h, it is preferred that testing time section most preferably 8:00AM—14:00PM, test temperature are 25 ± 1 DEG C, relatively
Humidity is 65% ± 5%.After test terminates, statistics traps the trypetid quantity in bottle, by the quantity of statistics and albumen bait formulation upper
State the quantity drawn in indoor bioassay result and compare that (albumen bait formulation in the present embodiment gives up for the homemade beer in laboratory
Yeast protein hydrolysate for obtaining after its self-dissolving, enzymolysis), accurately show luring for sample and albumen bait formulation to be apparent
The difference of effect, and the concentration of additive in sample is further optimized.As shown in table 1, use using 3 kinds of additives as three because
Element, three levels of three factors are its volumetric concentration in paraffin oil, and three level values of three factors be 20%,
25% and 30% orthogonal experimental method carries out above-mentioned indoor bioassay, in an experiment, the sex immature of each experimental group
Three kinds of additions in phase Bactrocera tau bait formulation are 50 μ L, and albumen bait formulation is 2mL, and each experimental group is with indoor biological test side
Formula is repeated 3 times test, and obtained experimental data takes the average value of 3 experiments.The orthogonal data analysis result of indoor measurement result
As shown in table 2：
The orthogonal experiment factor level table of table 1
The orthogonal experiment analytical table of table 2
As shown in table 2, wherein A represents the pungent fat of acetic acid, and B represents benzaldehyde solution, and C represents 3- methyl-1-butanol solution, D
For albumen bait formulation.Control group is 2.15mL albumen bait formulation, k corresponding to A factors rowM1Represent 3 first level experimental groups of A factors
Lure the average of trypetid number, k corresponding to A factors rowM2Represent that 3 the second horizontal experimental groups of A factors lure the average of trypetid number,
K corresponding to A factors rowM3Represent that 3 the 3rd horizontal experimental groups of A factors lure the average of trypetid number, corresponding to remaining factor
kM1、kM2、kM3And so on, similarly k♀1、k♀2、k♀3Represent that each female fly of level of each factor lures the average of quantity, k♂1、k♂2、k♂3
Represent that each male fly of level of each factor lures the average of quantity.Analytical table 2 directly perceived, can draw 9 groups the data neutrality immature phase South Asia
Trypetid bait formulation lures total amount minimum 27.3, wherein female is 11.9, more than not adding luring for additivated albumen bait formulation
Total amount 26.2, female fly quantity 11.3.Therefore it can show that 3 kinds of additives have enhancing albumen bait formulation epigamic not by said determination
The effect of maturity period Bactrocera tau.Additionally by the extreme difference of three factors of contrast, i.e. RMB＞ RMA＞ RMC, wherein RMBExpression factor A
On the extreme difference for luring total amount to influence, RMAExpression factor B is on luring the extreme difference that total amount influences, RMCExpression factor C is to luring total amount shadow
Loud extreme difference, show that 3 kinds of additive benzaldehydes are suitable to the influence primary and secondary of albumen bait formulation epigamic sex immature phase phase Bactrocera tau
Sequence is is followed successively by, octyl acetate, 3- methyl-1-butanols, best of breed A3B1C3, i.e., the octyl acetate of 30% volumetric concentration,
3- methyl isophthalic acids-butanol of the benzaldehyde of 20% volumetric concentration, 30% volumetric concentration, lures rate to reach 72.8%；For female adult
R♀AThe extreme difference that expression factor A influences on the female fly amount of luring, R♀BThe extreme difference that expression factor B influences on the female fly amount of luring, R♀CRepresent
The extreme difference that factor C influences on the female fly amount of luring, R♀B＞ R♀C＞ R♀AShow 3 kinds of reagents to the albumen bait formulation epigamic sex immature phase
The influence primary and secondary order of phase female Bactrocera tau is followed successively by benzaldehyde, 3- methyl-1-butanols, octyl acetate, and best of breed is
A1B2C2That is the octyl acetate of 20% volumetric concentration, the benzaldehyde of 25% volumetric concentration, 3- methyl isophthalic acids-fourth of 25% volumetric concentration
Alcohol, it is 62.0% that female adult, which lures rate,.According to the result of indoor bioassay, consider, because female adult harm is tighter in practice
Weight, therefore best of breed is A1B2C2That is the octyl acetate of 20% volumetric concentration, the benzaldehyde of 25% volumetric concentration, 25% volume
The 3- methyl-1-butanols of concentration, it is 36.4 that total amount is lured in the combination, and the female fly amount of luring is 15.1, and contrast protein stoste is drawn
Total amount 26.2, the female fly amount of luring 11.3 are lured, attractant effect has obvious enhancing.
The sex immature phase Bactrocera tau bait formulation of the present invention determines its attractant effect using outdoor test, and test mode is such as
Under：
The outdoor big cage of test：Specification 120cm × 120cm × 120cm, the mesh nylon grenadine of six face of plastic skeleton 100 covering seam
Close.
Trap bottle：Its upper end open uses the 50mL conical flasks of ParafilmTM, and its intrinsic upper end end is inserted in sealed membrane center
Portion is flush with sealed membrane top surface, the 1.5mL centrifuge tubes that lower end is in conical flask, the length about 3cm of centrifuge tube.
Under outdoor conditions (fine, calm), the being put into property sex immature phase Bactrocera tau 120 in the big cage of outdoor test
Head, each 60 of male and female, the central mounting of the outdoor big cage of test have three self-controls to trap bottle, wherein, first bottle trap bottle built with
The albumen bait formulation I that the benzaldehyde of its 6mL albumen bait formulation and/150 μ L20% volumetric concentrations is formulated, second bottle to trap bottle built-in
There are the octyl acetate, the benzaldehyde and 150 of the volumetric concentrations of 150 μ L 25% of its 6mL albumen bait formulation and 150 μ L20% volumetric concentrations
The albumen bait formulation II that the 3- methyl-1-butanols of the volumetric concentrations of μ L 25% are formulated, the 3rd bottle traps bottle built with 6.45mL eggs
White bait formulation；Bottle is trapped away from ground about 0.8m, test total duration is 10h, and test window of opportunity is 8:00A.M--18:00P.M, often
2h counts three trypetid quantity trapped in bottle, and above-mentioned outdoor test mode is repeated 3 times test, test result as shown in figure 1, by
Fig. 1 understands that the albumen bait formulation of three kinds of different disposals has attractant effect, attractant effect to the female male worm of sex immature phase Bactrocera tau
Power be followed successively by albumen bait formulation II, albumen bait formulation I, albumen bait formulation stoste.Show albumen bait formulation I, albumen bait formulation II relative to
Albumen bait formulation stoste has a synergistic effect, and the attractant effect of albumen bait formulation II be better than the explanation of albumen bait formulation I mixing additive relative to
The attractant effect of single additive is more notable； 10:00--12:00 this period lured more trypetid, and the female adult lured
Number is also more compared with other times section.
The present inventor is had found because cue-lure is good to the male fly attractant effect of Bactrocera tau, and upper in research process
There is preferable dissolubility in the sex immature phase Bactrocera tau bait formulation stated, will not be anti-with above-mentioned sex immature phase Bactrocera tau bait formulation
Should, therefore 2.5 parts need to be only added in sex immature phase Bactrocera tau bait formulation using vinegar as solvent, cue-lure is the volume of solute
Concentration is 25% cue-lure solution, can further improve the attractant effect of sex immature phase Bactrocera tau bait formulation.
The present inventor also found in research process only need to replace with benzyl carbinol and second by benzaldehyde and 3- methyl-1-butanols
Sour phenethyl ester, so that it may make the sex immature phase melonfly bait formulation excellent to sex immature phase melonfly attractant effect.
To sex immature phase melonfly bait formulation indoor bioassay
Above-mentioned indoor bioassay is used to above-mentioned sex immature phase melonfly bait formulation, by above-mentioned indoor bioassay
Sex immature phase Bactrocera tau replaces with sex immature phase melonfly, and the condition of remaining indoor bioassay is same as above, equally with just
Experimental method is handed over to carry out above-mentioned indoor bioassay, orthogonal experiment uses the L of Three factors-levels9(34) design of orthogonal gauge outfit,
In an experiment, three kinds of additions in the sex immature phase melonfly agent of each experimental group are 50 μ L, and control group is 2.15mL eggs
White bait formulation, comparison is made to each test combinations control sample from the method for above-mentioned indoor bioassay.Experimental result
Data processing it is as follows:
Attractant effect of the 33 kinds of reagent mixing additives of table to sex immature melonfly
As shown in table 3, wherein A represents the pungent lipoprotein solution of acetic acid, and B represents benzyl carbinol solution, and C represents phenethyl acetate solution,
4th is classified as k corresponding to 2mL albumen bait formulation A factors rowM1Represent that 3 first level experimental groups of A factors lure the equal of trypetid number
Value, k corresponding to A factors rowM2Represent that 3 the second horizontal experimental groups of A factors lure the average of trypetid number, corresponding to A factors row
kM3Represent that 3 the 3rd horizontal experimental groups of A factors lure the average of trypetid number, k corresponding to remaining factorM1、kM2、kM3It is such as such
Push away, similarly k♀1、k♀2、k♀3Represent that each female fly of level of each factor lures the average of quantity, k♂1、k♂2、k♂3Represent each level of each factor
Male fly lures the average of quantity.Analytical table 3 directly perceived, can draw 9 groups of data neutrality immature phase melonfly bait formulation lures total amount
Minimum 30.2, wherein female is 12.8, do not add additivated albumen bait formulation more than control group lures total amount 26.9, female fly
Quantity 11.7.Therefore it can show that melonfly bait formulation sex immature phase, melon epigamic immature phase of the present invention is real by said determination
The effect of fly is better than being not added with the albumen bait formulation of additive.Additionally by the extreme difference (R of three factors of contrastMBA is to drawing for expression factor
Lure the extreme difference of total amount, RMAExpression factor B is on luring the extreme difference that total amount influences, RMCExpression factor C is on luring the pole that total amount influences
Difference), RMB＞ RMA＞ RMCShow 3 kinds of reagents to the influence primary and secondary order of albumen bait formulation epigamic immature phase melonfly for successively
For benzyl carbinol, octyl acetate, phenethyl acetate, best of breed A1B3C3That is the octyl acetate of 20% volumetric concentration, 30% volume
The phenethyl acetate of the benzyl carbinol of concentration, 30% volumetric concentration, lures rate to reach 72.6%；For female adult R♀AExpression factor A
To the extreme difference of the female fly amount of luring, R♀BExpression factor B is to the extreme difference of the female fly amount of luring, R♀CExpression factor C is to the female fly amount of luring
Extreme difference, R♀A＞ R♀B＞ R♀CShow influence primary and secondary order of 3 kinds of reagents to albumen bait formulation epigamic immature phase female melonfly
It is followed successively by octyl acetate, benzyl carbinol, phenethyl acetate, best of breed A1B3C3That is the octyl acetate of 20% volumetric concentration, 30%
The phenethyl acetate of the benzyl carbinol of volumetric concentration, 30% volumetric concentration, it is 62.8% that female adult, which lures rate,；For male worm R♂ARepresent
Factor A is to the extreme difference of the male fly amount of luring, R♂BExpression factor B is to the extreme difference of the male fly amount of luring, R♂CExpression factor C draws to male fly
The extreme difference for the amount of luring, R♂B＞ R♂A＞ R♂CShow influence primary and secondary of 3 kinds of reagents to albumen bait formulation epigamic immature phase male melon flies
Order is to be followed successively by benzyl carbinol, octyl acetate, phenethyl acetate.According to the result of indoor bioassay, consider, due to reality
Female adult harm is more serious in border, therefore best of breed is A1B3C3That is the octyl acetate of 20% volumetric concentration, 30% volumetric concentration
The phenethyl acetate of benzyl carbinol, 30% volumetric concentration, it is 36.3 that total amount is lured in the combination, and the female fly amount of luring is 15.7, contrasts egg
White matter stoste lures total amount 26.9, the female fly amount of luring 11.7, and attractant effect has obvious enhancing.
To being determined outside sex immature phase melonfly bait formulation room
The outdoor big cage of test：Specification 120cm × 120cm × 120cm, the mesh nylon grenadine of six face of plastic skeleton 100 covering seam
Close.
Trap bottle：Its upper end open uses the 50mL conical flasks of ParafilmTM, and its intrinsic upper end end is inserted in sealed membrane center
Portion is flush with sealed membrane top surface, the 1.5mL centrifuge tubes that lower end is in conical flask, the length about 3cm of centrifuge tube.
Under outdoor conditions (fine, calm), the being put into property sex immature phase phase adult 120 in the big cage of outdoor test
Head, each 60 of male and female, the central mounting of the outdoor big cage of test have three self-controls to trap bottle, wherein, first bottle trap bottle built with
The albumen bait formulation A that its 6mL albumen bait formulation and the benzaldehyde of 450 μ L20% volumetric concentrations are formulated, second bottle to trap bottle built-in
There are octyl acetate, the benzyl carbinol and 150 μ of 150 μ L30% volumetric concentrations of its 6mL albumen bait formulation and 150 μ L20% volumetric concentrations
The albumen bait formulation B that the phenethyl acetate of L30% volumetric concentrations is formulated, the 3rd bottle traps bottle built with 6.45mL albumen baits
Agent stoste；Bottle is trapped away from ground about 0.8m, test total duration is 10h, the testing time 8:00A.M--18:00P.M, united per 2h
Meter traps the trypetid quantity in bottle.Above-mentioned outdoor test mode is repeated 3 times test, and test result is as shown in Figure 2.As shown in Figure 2,
The albumen bait formulation of three kinds of different disposals has attractant effect to the female male worm of sex immature phase melonfly, and the power of attractant effect is successively
For albumen bait formulation B, albumen bait formulation A, albumen bait formulation stoste.Show albumen bait formulation A, albumen bait formulation B relative to albumen bait formulation stoste
There is synergistic effect, and albumen bait formulation B attractant effects are better than albumen bait formulation A and show to mix additive drawing relative to single additive
Lure effect more notable；10:00--14:00 this period lured more trypetid, and the female borer population lured is compared with other times section
Also it is more.
The product form and style of above-described embodiment and schema and non-limiting patent of the present invention, any art
The appropriate change or modification that those of ordinary skill is done to it, it all should be regarded as not departing from the patent category of patent of the present invention.
Claims (4)
1. a kind of sex immature phase Bactrocera tau bait formulation, it is characterised in that mainly mixed by volumes below part component：
The above-mentioned pungent lipoprotein solution of acetic acid is using paraffin oil as solvent, and the pungent fat of acetic acid is that to make volumetric concentration be 20%-30% to solute
The pungent lipoprotein solution of acetic acid；
Above-mentioned benzaldehyde solution is that benzaldehyde is that solute makes the benzene that volumetric concentration is 20%-30% using paraffin oil as solvent
Formalin；
Above-mentioned 3- methyl-1-butanols solution is using paraffin oil as solvent, and 3- methyl-1-butanols are that solute makes volumetric concentration and is
20%-30% 3- methyl-1-butanol solution.
A kind of 2. sex immature phase Bactrocera tau bait formulation as claimed in claim 1, it is characterised in that：By volumes below part component
Mix：
The above-mentioned pungent lipoprotein solution of acetic acid is that the pungent fat of acetic acid is that solute makes the acetic acid that volumetric concentration is 20% using paraffin oil as solvent
Pungent lipoprotein solution；
Above-mentioned benzaldehyde solution is using paraffin oil as solvent, and it is molten that benzaldehyde is that solute makes the benzaldehyde that volumetric concentration is 25%
Liquid；
Above-mentioned 3- methyl-1-butanols solution is using paraffin oil as solvent, and 3- methyl-1-butanols are that solute makes volumetric concentration and is
25% 3- methyl-1-butanol solution.
3. a kind of preparation method of sex immature phase Bactrocera tau bait formulation, it is characterised in that be made by the steps and form：
(1) prepared by additive：
A, prepared by the pungent lipoprotein solution of acetic acid, and using paraffin oil as solvent, the pungent fat of acetic acid is that to make volumetric concentration be 20%-30% to solute
The pungent lipoprotein solution of acetic acid,
B, prepared by benzaldehyde solution, and using paraffin oil as solvent, benzaldehyde is that solute makes the benzene that volumetric concentration is 20%-30%
Formalin；
C, prepared by 3- methyl-1-butanols solution, and using paraffin oil as solvent, 3- methyl-1-butanols are that solute makes volumetric concentration
For 20%-30% 3- methyl-1-butanol solution；
(2) mix：The pungent lipoprotein solution of acetic acid, benzaldehyde solution and the 3- methyl-1-butanols solution and albumen bait formulation of step (1) are led to
Cross eddy mixer to mix together, mix to layering and disappear, obtain described sex immature phase Bactrocera tau bait formulation；Second
Misery lipoprotein solution, benzaldehyde solution, 3- methyl-1-butanols solution and above-mentioned albumen bait formulation are mixed by volumes below part：
A kind of 4. preparation method of sex immature phase Bactrocera tau bait formulation according to claim 3, it is characterised in that：It is above-mentioned
The volumetric concentration of the pungent lipoprotein solution of acetic acid is 20%, and the volumetric concentration of above-mentioned benzaldehyde solution is 25%, above-mentioned 3- methyl-1-butanols
The volumetric concentration of solution is 25%, parts by volume, the parts by volume of above-mentioned benzaldehyde solution and the above-mentioned 3- of the above-mentioned pungent lipoprotein solution of acetic acid
The parts by volume of methyl-1-butanol solution is 2.5 parts.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411327A (en) * | 2007-10-18 | 2009-04-22 | 东莞市盛唐化工有限公司 | Food agent for luring Bactrocera cucurbitae |
| CN101589728A (en) * | 2009-06-30 | 2009-12-02 | 福建农林大学 | Protein attractant for Bactrocera dorsalis Hendel and preparation method thereof |
| CN101690477A (en) * | 2009-10-15 | 2010-04-07 | 杜进平 | Food-sex attraction slow-release attractant for melon flies, and application thereof |
| CN104770371A (en) * | 2015-02-06 | 2015-07-15 | 温州医科大学 | Attractant for male and female fruit flies and melon flies |
| CN106900700A (en) * | 2017-03-15 | 2017-06-30 | 全国农业技术推广服务中心 | A kind of attractant for juxiaoshi fly |
-
2017
- 2017-08-08 CN CN201710674783.5A patent/CN107518005A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411327A (en) * | 2007-10-18 | 2009-04-22 | 东莞市盛唐化工有限公司 | Food agent for luring Bactrocera cucurbitae |
| CN101589728A (en) * | 2009-06-30 | 2009-12-02 | 福建农林大学 | Protein attractant for Bactrocera dorsalis Hendel and preparation method thereof |
| CN101690477A (en) * | 2009-10-15 | 2010-04-07 | 杜进平 | Food-sex attraction slow-release attractant for melon flies, and application thereof |
| CN104770371A (en) * | 2015-02-06 | 2015-07-15 | 温州医科大学 | Attractant for male and female fruit flies and melon flies |
| CN106900700A (en) * | 2017-03-15 | 2017-06-30 | 全国农业技术推广服务中心 | A kind of attractant for juxiaoshi fly |
Non-Patent Citations (2)
| Title |
|---|
| 杜迎刚等: "南亚实蝇和瓜实蝇对啤酒废酵母酶解液主要挥发性成分的触角电位反应", 《江苏农业科学》 * |
| 高扬等: "橘小实蝇蛋白饵剂田间试验初报", 《武夷科学》 * |
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