A kind of formula for improving rice grain circularity and its preparation method and application
Technical field
The invention belongs to Rice Production field, more particularly to a kind of formula for improving rice grain circularity and its preparation side
Method and application.
Background technology
It is increasingly serious with world food crisis, grain yield how is improved also into concerned one big heat
Point.Rice (Oryza sativa) is most important cereal crops in the world, provides the energy more than 21% for world population, is
South east asia population provides up to 76% energy (Melissa et al.2009).Therefore, rice yield is improved, to ensureing
World food is significant safely.The circularity height of rice is one of key factor for forming rice yield.
Rice grain circularity refers to final filling extent of the development ovary (grain of rice) in husk, and it includes two aspects
Implication:First, grain of rice volume accounts for the ratio of husk maximum volume, ratio is bigger, and circularity is just higher;Second, thing inside the grain of rice
The compactness extent of matter (mainly starch) structure, inner material structure is closer, and grain-filling degree is then higher.
The calculation formula of general grain-filling degree is:
Grain-filling degree (%)=100 × (real grain weight+blighted grain weight of the processing per fringe)/(the real grain weight of respective amount).
Full grain rate can be effectively improved by improving the circularity of rice grain.The height of grain-filling degree depends on kind in itself
Grain weight and the grain of productive phase weight incrementss.Although grain weight is influenceed by many factors, kind is largely determined by.Grain weight incrementss
Influenceed by Filling material supply, seed Capacity activity, dispensed materials and various environmental factors, but finally show grouting speed
On degree and grouting time.The height of grain weight determined by the development degree of husk size and endosperm, the development degree of endosperm and by
Blooming early, inserted part, Filling material level of supply, cultivation condition etc. influence late, on fringe.(Wang Yulong etc. 1995,1998).
Nagato (1941) reports that different parts kernel grouting has differences on spike of rice first, and the grain husk flower early opened is referred to as strong
Gesture grain, grain weight is high, raw in spike of rice middle and upper part;The grain husk flower opened late is referred to as Inferior grain, and grain weight is low, raw in spike of rice bottom.Weak tendency
Grain enriches difference and the low not only limitation crop yield of grain weight is dived the performance for being, can also have a strong impact on grain quality.At present, improve and enrich
The common method of degree includes the methods of Dry-wet alternate irrigation technology, chemical substance (plant growth regulator) regulation and control, such as in early rice
Beginning ear period and full heading time spray exogenous hormone plant growth regulator GA3+6-BA+BR, can improve kernel grouting speed, promote sugarcane
The accumulation of conversion and starch of the sugar to starch, so as to improve circularity and yield.But these methods one be effect be not it is obvious that
Second, chemical substance is possible to impact environment in processing procedure.
The content of the invention
In place of solving shortcoming and defect present in above-mentioned prior art, primary and foremost purpose of the invention is to provide one
Kind improves the formula of rice grain circularity;The formula can by be used alone or by with the field during Rice Production
Between control measures combine to improve rice grain circularity.
Another object of the present invention is to provide the preparation method of above-mentioned formula.
It is still another object of the present invention to provide the application of above-mentioned formulation product.
The purpose of the present invention is realized by following technical proposals:
A kind of formula for improving rice grain circularity, the formula are made up of following raw material by mass percentage:
Preferably, the formula is made up of following raw material by mass percentage:
The Ampelopsis grossedentata extrat dihydromyricetin, refer to using vine tea as raw material, by extracting, purifying obtained product, its
Weight/mass percentage composition >=55% of middle dihydromyricetin;
The RV, refer to using Grape Skin, grape pip or giant knotweed as raw material, by extracting, purifying obtained product,
Wherein weight/mass percentage composition >=20% of RV;
The weight/mass percentage composition of vitamin E is more than 95% in the vitamin E;
Ascorbic weight/mass percentage composition is more than 95% in the vitamin C
The weight/mass percentage composition of the agricultural streptomycin streptomycin is more than 70%.
The Ampelopsis grossedentata extrat dihydromyricetin is to be prepared according to the following steps:Using the fresh cauline leaf of vine tea, in temperature
Vine tea dry feedstock is obtained after being dried under the conditions of 35~45 DEG C of degree;Vine tea dry feedstock is obtained into vine tea using ethanol extraction method to carry
Take liquid, the 30~40% of concentrated extracting solution to its original volume, and concentrate is placed on-Cool Room 4 DEG C in stand 24 hours, dry
Liquid bottom, which precipitates, produces dihydromyricetin extract;The mass ratio of vine tea dry feedstock and ethanol in the ethanol extraction method
For 1:10~12, the concentration of volume percent of ethanol is 85~95%;The vine tea uses Guangdong, Guangxi, Hunan, Hubei or river
The vine tea of west production.
Preferably, the ethanol concentration of volume percent 90%;It is by concentrate bottom that the dry liquid bottom precipitation, which is,
Being deposited in 90~95 DEG C of baking oven for portion is dried;The vine tea uses the vine tea produced in Hunan Zhangjiajie spring and summer season.
The RV is to be prepared according to the following steps:Using giant knotweed as raw material, with the volume basis of 6 times of volumes of raw material
The ethanol that number is 75% extracts 1 hour, concentration and recovery ethanol, spraying is dry as Extraction solvent in the case of being 70-75 DEG C in temperature
Dry extract solution, produces RV.
The vitamin E produces for Zhengzhou dragon food Chemical Co., Ltd.;The vitamin C is that the really prosperous chemical industry in Zhengzhou has
Limit company produces;The agricultural streptomycin produces for Sichuan Chengdu Pu Hui bioengineering Co., Ltd.
The preparation method of the formula of above-mentioned raising rice grain circularity, including following operating procedure:Claim by said ratio
Take Ampelopsis grossedentata extrat dihydromyricetin, RV, vitamin E, vitamin C and agricultural streptomycin to be placed in blend tank, stir
Uniformly, the formula for improving rice grain circularity is packed or produced after canned to compositing aluminium bag.
Application of the above-mentioned formula in rice grain circularity is improved.
The application includes following operating procedure:In the premilk stage of rice milking stage solid period, fine day is selected, will with water
It is that the direct of 0.5-1.0% uses liquid that the formulation product stirring and dissolving of raising rice grain circularity, which is allowed to form mass percent,
It will directly be sprayed by 50-60 kgs/acre of dosage with liquid by the method for foliage-spray on rice leaf, 1 sprayed during milking maturity
It is secondary;Rainy weather is such as run into 24 hours after spraying, then needs repeat spraying once.
The present invention principle be:
Ampelopsis grossedentata extrat dihydromyricetin is the main active in vine tea plant, has anti-aging, anti-oxidant, promotion
A variety of effects such as cell balance growth.The main function of dihydromyricetin is the milking maturity in rice milking stage solid period in this formula
Phase, it is transferred in rice grain by Foliage Absorption, balance different parts grain filling rate on Rice Panicle, promote weak tendency seed
The quick grouting of grain, the ratio of surging seed on Rice Panicle is improved, so as to the overall circularity for improving rice grain.
RV:Resveratrol is a kind of polyphenol compound, is mainly derived from peanut, grape (red wine), tiger
The plants such as cane, mulberry fruit, there is treatment inflammation, mitigate the effect such as disorders of lipid metabolism, anti-oxidant.Bai Lilu is used in this formula
Alcohol, mainly promote the filling speed of weak tendency seed using it.
Vitamin E (Vitamin E) is a kind of liposoluble vitamin, and its hydrolysate is tocopherol, is most important anti-
One of oxidant.Using conveniently can mainly enter using it in lipid cell membrane in this formula, the lipid generation of blastocyte is participated in
Thank, eliminate the serial free radical in metabolic process, reach the purpose for improving rice grain circularity.
Vitamin C:For water-soluble powerful antioxidant.Using mainly cooperateing with work with vitamin E using it in this formula
With, with this reach improve rice grain circularity purpose..
Agricultural streptomycin:It is soluble in water, it is antibiotic medicament.Main purpose is to prevent and treat damage of the rice disease to rice
Evil, so as to improve rice grain circularity.
The present invention compared with the prior art, has the following advantages and beneficial effect:
(1) inventive formulation is reasonable, fully utilizes in chemical raw material and natural plants extract to improving Grain Filling
The respective effect of degree, experiments verify that to improving rice grain circularity effect clearly;
(2) processing and fabricating is simple, easy to use, and a kind of preferable medicine is provided to improve rice grain circularity.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Each raw material sources are as follows used in following examples:
1st, Ampelopsis grossedentata extrat dihydromyricetin is to be prepared according to the following steps:Using the fresh cauline leaf of vine tea, in temperature
Vine tea dry feedstock is obtained after being dried under the conditions of 35~45 DEG C;Vine tea dry feedstock is obtained into vine tea extraction using ethanol extraction method
Liquid, the 30~40% of concentrated extracting solution to its original volume, and concentrate is placed on-Cool Room 4 DEG C in stand 24 hours, dry liquid
Body bottom precipitation produces dihydromyricetin extract;The mass ratio of vine tea dry feedstock and ethanol is in the ethanol extraction method
1:10~12, the concentration of volume percent of ethanol is 85~95%, preferred volume percent concentration 90%.The vine tea is using wide
The vine tea of the real estates such as east, Guangxi, Hunan, Hubei, Jiangxi, the vine tea that preferably Hunan Zhangjiajie spring and summer season produces.It is described
Dry liquid bottom precipitation is to dry being deposited in 90~95 DEG C of baking oven for concentrate bottom.Gained dihydromyricetin extracts
In produce product, weight/mass percentage composition >=55% of dihydromyricetin.
2nd, RV is to be prepared according to the following steps:Using the giant knotweed of RV rich content as raw material, add raw material 6
The ethanol that the percent by volume of times volume is 75% is used as Extraction solvent, is extracted 1 hour in the case of temperature is 70-75 DEG C, dense
Retract and receive ethanol, be spray-dried extract solution, produce RV extract.In RV extract, the quality of RV
Percentage composition >=20%.
3rd, vitamin E produces for Zhengzhou dragon food Chemical Co., Ltd., and weight/mass percentage composition is more than 95%.
4th, vitamin C produces for Zhengzhou Cheng Wang Chemical Co., Ltd.s, and weight/mass percentage composition is more than 95%.
5th, agricultural streptomycin, produced for Sichuan Chengdu Pu Hui bioengineering Co., Ltd, the weight/mass percentage composition of streptomysin
For 72%.
Embodiment 1:
The Ampelopsis grossedentata extrat dihydromyricetin, mass percent 4.26% that mass percent is 63.82% are weighed respectively
RV, mass percent be 10.64% vitamin E, mass percent be 10.64% vitamin C and quality hundred
Point than being placed in for 10.64% 5 kinds of raw materials of agricultural streptomycin in blend tank, stir, compositing aluminium bag packaging or it is canned after i.e.
The formulation product of grain-filling degree must be improved.
Embodiment 2:
The Ampelopsis grossedentata extrat dihydromyricetin, mass percent 5.00% that mass percent is 62.00% are weighed respectively
RV, mass percent be 11.00% vitamin E, mass percent be 11.00% vitamin C and quality hundred
Point than being placed in for 11.00% 5 kinds of raw materials of agricultural streptomycin in blend tank, stir, compositing aluminium bag packaging or it is canned after i.e.
The formulation product for improving grain-filling degree must be produced.
Embodiment 3:" 368 " serial hybridization rice sprays the change of grain-filling degree after raising grain-filling degree formulation product
Situation
Weigh embodiment 1 preparation product 500g, be allowed into emulsion with 50kg water stirring and dissolvings, with Plastic Drum it is filling after
Produce the raising grain-filling degree formulation product that mass percent is 1% directly uses liquid.By it is above-mentioned directly with liquid " wide 8 is excellent
368 " and " excellent 368 " the hybrid rice productive experiment field in day (under natural growthing condition, rice premilk stage sprays) sprays 1 time, spray
Usage amount is applied directly to use liquid 50kg/ mus, every piece of experimental field area is 0.1 mu.In addition, spraying directly with the hybrid rice of liquid
Production crop field adjacent zone sets the control CK for not spraying and directly using liquid.After rice harvest, by " rice grain circularity calculates public
Formula " carries out circularity measure to it.
Grain-filling degree (%)=(the average mass of 1000 kernel of each rank seed/full grain seed mass of 1000 kernel) × 100
Table 1 below is data variation (the Hybrid Rice Combinations material of 2 " 368 " serial hybridization Rice Production crop field circularity
There is provided by Golden Rice Seeds Co., Ltd. of Guangdong), there it can be seen that the grain-filling degree sprayed after product of the present invention substantially carries
It is high.
Different " 368 " the serial hybridization rice of table 1 spray the situation of change of grain-filling degree after this formulation product
Embodiment 4:Hybrid rice sprays the situation of change of grain-filling degree after raising grain-filling degree formulation product
The product 750g of the preparation of embodiment 2 is weighed, emulsion is allowed into 100kg water stirring and dissolvings, it is filling with Plastic Drum
Produce the raising grain-filling degree formulation product that mass percent is 0.75% afterwards directly uses liquid.Liquid is directly used in water by above-mentioned
Rice premilk stage is sprayed in " safe Feng You 208 " and each 1 time of " wide 8 excellent China accounts for " hybrid rice productive experiment Tanaka.Every piece of experimental field
Area is 0.1 mu, sprays usage amount every time and directly uses liquid for 50kg/ mus.In addition, directly produced spraying with the hybrid rice of liquid
Experimental field adjacent zone sets the control CK for not spraying and directly using liquid.After rice harvest, by " rice grain circularity calculation formula "
Circularity measure is carried out to it.
Grain-filling degree (%)=(the average mass of 1000 kernel of each rank seed/full grain seed mass of 1000 kernel) × 100
Table 2 below is that 2 hybrid rice produce the data variation of crop field circularity (Hybrid Rice Combinations material is by Guangdong Province's gold
Seed rice industry Co., Ltd provides), there it can be seen that the grain-filling degree sprayed after product of the present invention significantly improves.
The hybrid rice of table 2 sprays the situation of change of grain-filling degree after this formulation product
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.