CN114557353B - Composition for regulating harvesting period of rice seeds and preparation method and application thereof - Google Patents

Composition for regulating harvesting period of rice seeds and preparation method and application thereof Download PDF

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CN114557353B
CN114557353B CN202210226594.2A CN202210226594A CN114557353B CN 114557353 B CN114557353 B CN 114557353B CN 202210226594 A CN202210226594 A CN 202210226594A CN 114557353 B CN114557353 B CN 114557353B
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seeds
composition
rice
extract
regulating
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CN114557353A (en
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高家东
刘军
付华
张友胜
马国辉
吴志媛
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Agro-Biological Gene Research Center Guangdong Academy Of Agricultural Sciences
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Agro-Biological Gene Research Center Guangdong Academy Of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • A01G22/22Rice
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
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  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the field of rice seeds, and discloses a composition for regulating and controlling a harvesting period of rice seeds, and a preparation method and application thereof. The composition is prepared from the following raw materials in percentage by mass: 61.50 to 63.50 percent of dihydromyricetin extract, 6.00 to 9.00 percent of resveratrol extract, 12.00 to 16.00 percent of vitamin C and 12.00 to 16.00 percent of prohexadione calcium. The invention has reasonable formula, comprehensively utilizes the respective effects of chemical raw materials and extracts of natural plants on controlling the harvest period of the seeds, and has obvious effect on controlling the harvest period of the rice seeds through experimental verification; the preparation method is simple, the use is convenient, and an ideal medicine is provided for regulating and controlling the harvesting period of rice seeds.

Description

Composition for regulating harvesting period of rice seeds and preparation method and application thereof
Technical Field
The invention belongs to the field of rice seeds, and particularly relates to a composition for regulating and controlling a harvesting period of rice seeds, and a preparation method and application thereof.
Background
The rice seeds are the most basic and irreplaceable production data in the rice production process, and the quality of the rice seeds determines the success or failure of field production, wherein timely mature harvesting of the seeds is an important factor for determining the quality of the seeds. In general, the harvest period of crop seeds has remarkable correlation with the germination rate, vigor of the seeds so as to ensure that the seedlings are strong and have growth conditions after use, and therefore, timely harvesting of the seeds is a basic requirement in the crop seed production process. In recent years, with the expansion of seed production scale, the change of seed production mode and the frequent occurrence of extreme weather, adverse conditions of poor weather but the necessity of harvesting seeds are frequently encountered in the seed production process. Under the condition of bad weather, the seeds are harvested, firstly, the harvest work is inconvenient, secondly, the harvested seeds are easy to produce stack retting, mildew pollution and other conditions due to weather reasons, especially the influence of rainy weather, so that the quality of the seeds is greatly influenced, and direct loss is brought to field production. Yet another disadvantage is that large areas of seeds need to be harvested at the same time. The condition of harvesting large-area seeds simultaneously brings much inconvenience to the arrangement of harvesting machinery and personnel, and seriously affects the quality of seeds which are not harvested in time. Therefore, the timely harvest of seeds in a staggered period is a constant dream for seed production agricultural producers. Especially, with the improvement of the working prediction accuracy of weather forecast, products capable of regulating and controlling the harvesting period of rice seeds are developed to become hot products in the rice seed production market, but the technical products have not been broken through.
Disclosure of Invention
In order to solve the above-mentioned disadvantages and drawbacks of the prior art, a primary object of the present invention is to provide a composition for controlling a harvesting period of rice seeds, which has the effect of controlling the harvesting period of rice seeds so that the seeds reach a desired seed germination rate at a proper time so as to harvest the seeds under proper time and weather conditions.
It is another object of the present invention to provide a process for preparing the above composition.
It is a further object of the present invention to provide the use of the above composition for regulating the harvest cycle of rice seeds.
The aim of the invention is achieved by the following technical scheme:
a composition for regulating the harvest time of rice seeds, which is prepared from the following raw materials in percentage by mass:
preferably, the composition is prepared from the following raw materials in percentage by mass:
the dihydromyricetin extract is a product obtained by taking vine tea as a raw material and extracting and purifying, wherein the mass percentage of the dihydromyricetin is more than or equal to 55%;
the Bai Lilu alcohol extract is a product obtained by taking grape skin and grape seeds as raw materials and extracting and purifying, wherein the mass percentage of resveratrol is more than or equal to 20%;
the mass percentage of the vitamin C in the vitamin C is more than 95 percent;
the prohexadione calcium is wettable powder with the volume percentage of prohexadione calcium accounting for 85%.
The dihydromyricetin extract is prepared by the following steps: the fresh stem leaves of vine tea are adopted, and dried at the temperature of 35-45 ℃ to obtain vine tea dried raw materials; ethanol extraction is adopted to the vine tea dry raw material to obtain vine tea extract, the extract is concentrated to 30-40% of the original volume, the concentrated solution is placed in a refrigeration house at-4 ℃ for standing for 24 hours, and the dihydromyricetin extract is obtained by precipitation at the bottom of the dry liquid; the mass ratio of the vine tea dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, the volume percentage concentration of ethanol is 85-95%; the vine tea is produced by Guangdong, guangxi, hunan, hubei or Jiangxi. Preferably, the ethanol volume percent concentration is 90%; the precipitation at the bottom of the drying liquid is that the precipitation at the bottom of the concentrated solution is dried in an oven at 90-95 ℃; the vine tea is produced in spring and summer in the area of Zhang Jiang Hunan.
The Bai Lilu alcohol extract is prepared by the following steps: extracting rhizoma Polygoni Cuspidati with 75% ethanol at 70-75deg.C for 1 hr, concentrating, recovering ethanol, and spray drying to obtain resveratrol extract.
The vitamin C is produced by Chengwang chemical industry Co., ltd.
The preparation method of the composition for regulating the harvesting period of rice seeds comprises the following operation steps: weighing the raw materials according to the proportion, placing the raw materials into a blending tank, uniformly stirring, and packaging or canning by a composite aluminum bag to obtain the composition for regulating and controlling the harvesting period of rice seeds.
The application of the composition for regulating the harvesting period of rice seeds in regulating the harvesting period of rice seeds is characterized in that: the application comprises the following operation steps: on the day after the artificial pollination of the rice seeds, the composition for regulating the harvest period of the rice seeds is stirred and dissolved by water to form a direct liquid with the mass percent of 0.1-0.3%, and the direct liquid is sprayed on rice ears according to the dosage of 50 kg/mu. The spraying can be selected for 1-2 times according to the time required to be regulated in the seed harvesting period.
Preferably, if the air is rainy in 24 hours after spraying, the spraying is required to be repeated once.
The principle of the invention is as follows:
dihydromyricetin is the main active ingredient in vine tea plant and has the functions of resisting senility, resisting oxidation, promoting cell to grow in balance, etc. The dihydromyricetin in the formula has the main effect of promoting the activities of Peroxidase (POD), catalase (CAT), dehydrogenase and amylase in seeds and increasing the content of soluble proteins in the rice seeds in the development process of the seeds.
Resveratrol is a phytoncide, an antioxidant, and also a histone modifying enzyme, and has an effect of accelerating assimilation transport. The formula mainly uses the sterilization effect, reduces the damage of microorganisms, enhances the stress capability of seed embryo cells and accelerates the transportation of assimilates, thereby shortening the seed harvesting period.
Vitamin C is a water-soluble strong antioxidant. On the one hand, the anti-stress capability and the immunity of the seeds to the external environment in the development process are enhanced by using the anti-stress agent in the formula, so that the germination vigor of the seeds is improved as a whole.
Prohexadione calcium: the alias was calcium cyclohexanone. The prohexadione calcium is used for lodging resistance of rice in japan at the earliest time, and is used for controlling vigorous growth of lawns and fruit trees and preventing and treating fire blight of fruit trees in europe and america later. Later, more systematic researches in China show that the prohexadione calcium can increase the chlorophyll content and enhance photosynthesis; promoting flower bud differentiation, improving fruit setting rate, promoting fruit expansion, and sweetening and coloring; promoting the expansion of tubers, improving the dry matter content and storability, increasing the yield, improving the quality and preventing premature senility. In the formula of the invention, when the prohexadione calcium is sprayed to rice ears, the prohexadione calcium can be quickly absorbed by crop leaf cells and rice ears and directly acts on plant gibberellin synthesis targets (gibberellin synthesis sites are in the leaves) to regulate and control the seed harvest period.
Compared with the prior art, the invention has the following advantages:
(1) The composition has reasonable formula, comprehensively utilizes the respective effects of chemical raw materials and extracts of natural plants on controlling the harvest period of seeds, and has obvious effect on controlling the harvest period of rice seeds through experimental verification;
(2) The preparation method is simple, the use is convenient, and an ideal medicine is provided for regulating and controlling the harvesting period of rice seeds.
Detailed Description
The present invention will be described in further detail with reference to examples, but embodiments of the present invention are not limited thereto.
1. All rice seeds during the experiment were supplied by the Long Ping company in Hunan.
2. The treatment method of the harvest time regulator during the rice seed production comprises the following steps: after artificial pollination (about 7d after 'Jiutwo' is sprayed) is finished on the rice in the seed production field, simultaneously, seed harvest time regulating agents (taking sprayed clear water as a control) are sprayed on the rice ears and the leaf surfaces respectively. Sampling is started 10 days after the regulator is sprayed, then samples are taken every 3 days, and indexes such as germination rate and germination vigor of the samples are detected respectively. The test combination was long two you 534 of the Long Ping industries in Hunan.
3. The method for measuring the germination vigor of rice seeds comprises the following steps: the method is carried out according to the rice seed germination rate measurement method (GB/T3543), and the result of the seed germination rate counted on the 4 th day after the germination experiment is started is the seed germination vigor.
4. The method for measuring the germination rate of rice seeds comprises the following steps: the germination rate (%) =number of germinated seeds/total number of seeds×100% was measured by the "rice seed germination rate measurement method" (GB/T3543), and the result of statistics on day 7 after the start of the germination experiment was taken in this experiment.
5. Seed mildew rate (%) =mildew seed number/total seed number×100%.
6. The sources of the raw materials used in the following examples are as follows:
the dihydromyricetin extract is prepared by the following steps: the fresh stem leaves of vine tea are adopted, and dried at the temperature of 35-45 ℃ to obtain vine tea dried raw materials; ethanol extraction is adopted on vine tea drying raw materials to obtain vine tea extracting solution, the extracting solution is concentrated to 30-40% of the original volume of the vine tea extracting solution, the concentrated solution is placed in a refrigeration house at-4 ℃ for standing for 24 hours, the sediment at the bottom of the drying liquid is obtained to obtain the dihydromyricetin extract, and the sediment at the bottom of the drying liquid is dried in an oven at 90-95 ℃; the mass ratio of the vine tea dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%, preferably ethanol with the volume percentage concentration of 90%; the vine tea is produced by Guangdong, guangxi, hunan, hubei or Jiangxi, and preferably produced in spring and summer in Zhang Jiang region of Hunan. The mass percentage of the dihydromyricetin in the obtained dihydromyricetin extract is more than or equal to 55 percent.
The resveratrol extract is prepared by the following steps: extracting rhizoma Polygoni Cuspidati with 75% ethanol as extraction solvent at 70-75deg.C for 1 hr, concentrating, recovering ethanol, and spray drying to obtain resveratrol extract with resveratrol content of more than or equal to 20%.
Vitamin C is produced by Chengwang chemical industry Co., ltd, and the mass percent is more than 95%;
the agricultural streptomycin sulfate is produced by Sichuan Chengdu general bioengineering Co., ltd, and the mass percentage content is more than 70%;
the prohexadione calcium is wettable powder with the volume percent of prohexadione calcium accounting for 85 percent.
Example 1:
the preparation method comprises the steps of respectively weighing four raw materials of 63% of dihydromyricetin extract, 7% of resveratrol extract, 15% of vitamin C and 15% of agricultural streptomycin sulfate, placing the four raw materials into a blending tank, uniformly stirring, and packaging or canning by a composite aluminum bag to obtain the composition (hereinafter referred to as a rice seed harvesting period regulator) for regulating and controlling the rice seed harvesting period.
Example 2:
the preparation method comprises the steps of respectively weighing four raw materials of 63% of dihydromyricetin extract, 7% of resveratrol extract, 15% of vitamin C and 15% of prohexadione calcium, placing the four raw materials into a blending tank, uniformly stirring, and packaging or canning by a composite aluminum bag to obtain the composition (hereinafter referred to as a rice seed harvesting period regulator) for regulating and controlling the rice seed harvesting period.
Example 3: regulation experiment of rice seed harvest period regulator on rice seed harvest period
The two rice seed harvest period regulators prepared in the above example 1 and experimental example 2 were respectively dissolved with water under stirring to form a direct solution with a mass percentage of 0.3%, and then the regulators were sprayed 1 time after the artificial pollination was completed in the rice seed production process, respectively, with the treatment of spraying clear water as a control. As can be seen from Table 1, the samples from 10 days to 24 days after pollination are taken, the germination rate and germination vigor of the early seeds can be obviously improved by using the two rice seed harvest period regulators, namely, the harvest period of the seeds is regulated, and meanwhile, the mildewing rate of the seeds is reduced and the overall quality of the seeds is improved after the rice seed harvest period regulators are sprayed. The experimental group subjected to the spraying treatment using the rice seed harvest time regulator prepared in example 1 was referred to as treatment a, and the experimental group subjected to the spraying treatment using the rice seed harvest time regulator prepared in example 2 was referred to as treatment B.
The germination vigor and germination rate of the seed sample 10 days after pollination are 65.33% and 75.33% for the control, and are significantly higher than for the control, wherein the germination vigor and germination rate of treatment A are 72.33% and 86% respectively, and the germination vigor and germination rate of treatment B are 78.67% and 86% respectively. According to the national standard, when the germination rate of the hybrid rice seeds reaches more than 80%, the seeds can be sold as qualified seeds. That is, in special extreme cases, the seeds after the regulator is sprayed can be harvested for field production; the germination rate of the control sample does not meet the national standard requirements until 13 days after pollination (3 days later than the sample sprayed with the regulator). In addition, the germination rate of the control was 92.33% 13 days after the pollination was completed, the germination rates of treatment a and treatment B were 93.33% and 95.67%, respectively, and the germination rate of treatment B was highest. Therefore, the regulator of the invention not only can regulate the harvest period of rice seeds, but also can improve the overall quality of the seeds. It is important to note that samples from day 16 and day 19 after pollination were significantly different (P < 0.05) for both treatments A and B compared to the control fresh water treated samples, indicating that the conditioner had significant conditioning effect on the seed harvest period. There was no significant difference between the two treatments.
Table 1 influence of rice seed harvest period regulator on germination vigor, germination rate and mildew rate of seeds at different periods after pollination
Note that: CK is a clear water control and treatments a and B are the modulator products of experimental examples 1 and 2, respectively. The tested combination was long two excellent 534.
Example 4: the composition for regulating the harvest period of the rice seeds is utilized to carry out simulated regulation and control on the harvest period of the rice seeds
In the rice seed production process, according to the difference of rice varieties, if the normal harvest period of a certain variety of seeds can be obtained after 15 days of pollination, but according to weather forecast, bad weather more than 5 continuous days occurs after 15 days or the harvest work of the seeds is busy after 15 days, the time is staggered, the composition for regulating the harvest period of the rice seeds can be sprayed at the right end of artificial pollination or at different times after artificial pollination is selected, so as to promote the seeds to reach a specified bud rate in advance, and the seeds can be harvested in advance after 10 days of pollination or other times so as to reduce loss or stagger the busy season.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.

Claims (9)

1. Use of a composition for regulating a rice seed harvest stage in regulating a rice seed harvest stage, characterized in that: the composition is prepared from the following raw materials in percentage by mass:
2. the use according to claim 1, characterized in that: the composition is prepared from the following raw materials in percentage by mass:
3. the use according to claim 1, characterized in that: the dihydromyricetin extract is a product obtained by taking vine tea as a raw material and extracting and purifying, wherein the mass percentage of the dihydromyricetin is more than or equal to 55%;
the Bai Lilu alcohol extract is a product obtained by taking grape skin and grape seeds as raw materials and extracting and purifying, wherein the mass percentage of resveratrol is more than or equal to 20%;
the mass percentage of the vitamin C in the vitamin C is more than 95 percent;
the prohexadione calcium is wettable powder with the volume percentage of prohexadione calcium accounting for 85%.
4. The use according to claim 1, characterized in that: the dihydromyricetin extract is prepared by the following steps: the fresh stem leaves of vine tea are adopted, and dried at the temperature of 35-45 ℃ to obtain vine tea dried raw materials; ethanol extraction is adopted to the vine tea dry raw material to obtain vine tea extract, the extract is concentrated to 30-40% of the original volume, the concentrated solution is placed in a refrigeration house at-4 ℃ for standing for 24 hours, and the dihydromyricetin extract is obtained by precipitation at the bottom of the dry liquid; the mass ratio of the vine tea dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, the volume percentage concentration of ethanol is 85-95%; the vine tea is produced by Guangdong, guangxi, hunan, hubei or Jiangxi.
5. The use according to claim 4, characterized in that: the volume percentage concentration of the ethanol is 90%; the precipitation at the bottom of the drying liquid is that the precipitation at the bottom of the concentrated solution is dried in an oven at 90-95 ℃; the vine tea is produced in spring and summer in the area of Zhang Jiang Hunan.
6. The use according to claim 1, characterized in that: the Bai Lilu alcohol extract is prepared by the following steps: extracting rhizoma Polygoni Cuspidati with 75% ethanol at 70-75deg.C for 1 hr, concentrating, recovering ethanol, and spray drying to obtain resveratrol extract.
7. The use according to claim 1, characterized in that: the composition is prepared according to the following operation steps: weighing the raw materials according to the proportion, placing the raw materials into a blending tank, uniformly stirring, and packaging or canning by a composite aluminum bag to obtain the composition for regulating and controlling the harvesting period of rice seeds.
8. The use according to claim 1, characterized in that: the application comprises the following operation steps: on the day after artificial pollination of rice seeds, the composition for regulating the harvest period of the rice seeds is stirred and dissolved by water to form direct liquid with the mass percent of 0.1-0.3%, the direct liquid is sprayed on rice ears according to the dosage of 50 kg/mu, and the spraying is carried out for 1-2 times according to the time required to be regulated in the harvest period of the seeds.
9. The use according to claim 8, characterized in that: if the air is rainy in 24 hours after spraying, the spraying is needed to be repeated once.
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CN114557352B (en) * 2022-03-09 2024-02-02 广东省农业科学院农业生物基因研究中心 Composition for promoting early harvesting of rice seeds as well as preparation method and application thereof

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