CN107417400B - Nutrient for improving content of gamma-aminobutyric acid in germinated brown rice and preparation method and application thereof - Google Patents
Nutrient for improving content of gamma-aminobutyric acid in germinated brown rice and preparation method and application thereof Download PDFInfo
- Publication number
- CN107417400B CN107417400B CN201710716651.4A CN201710716651A CN107417400B CN 107417400 B CN107417400 B CN 107417400B CN 201710716651 A CN201710716651 A CN 201710716651A CN 107417400 B CN107417400 B CN 107417400B
- Authority
- CN
- China
- Prior art keywords
- brown rice
- gamma
- aminobutyric acid
- content
- germination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/152—Cereal germ products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cereal-Derived Products (AREA)
Abstract
The invention discloses a nutritional agent for improving the content of gamma-aminobutyric acid in germinated brown rice, a preparation method and application thereof; the raw materials comprise the following components in percentage by mass: 47.50-48.50% of vine tea extract, 15.50-16.00% of rosemary extract, 214.25-14.30% of vitamin B, 10.00-15.00% of sodium glutamate, 64.15-4.9% of vitamin B, 4.15-4.9% of vitamin C and 2.3-3.40% of folic acid. Calculated by the content of the gamma-aminobutyric acid in the whole germinated brown rice, the content of the gamma-aminobutyric acid in the brown rice soaked in the nutrient solution reaches above 401.2mg/100g of brown rice. The nutrient solution of the invention has no toxicity and low price.
Description
Technical Field
The invention relates to gamma-aminobutyric acid, and particularly relates to a nutritional agent for improving the content of gamma-aminobutyric acid in germinated brown rice, and a preparation method and application thereof.
Background
Gamma-aminobutyric acid is an active ingredient widely present in animals and plants. The modern medicine proves that the gamma-aminobutyric acid has the functions of tranquilizing nerves, resisting anxiety, reducing blood pressure, reducing blood ammonia, improving brain activity, promoting ethanol metabolism, preventing skin aging, eliminating body odor, improving lipid metabolism, preventing arteriosclerosis, efficiently losing weight and the like. People usually use the germinated brown rice to improve the content of the gamma-aminobutyric acid in the staple food so as to achieve the purposes of taking more gamma-aminobutyric acid and building the body.
Gamma-aminobutyric acid belongs to nonprotein amino acid, protein in brown rice embryo is hydrolyzed into amino acid under the action of endogenous protease, wherein, the glutamic acid is generated into the gamma-aminobutyric acid under the action of glutamate decarboxylase, and the coenzyme of the gamma-aminobutyric acid is pyridoxal phosphate. In addition, the gamma-aminobutyric acid synthesis site is mainly present in the embryo. Therefore, any substance which affects the synthesis and metabolism of gamma-aminobutyric acid such as glutamic acid, enzymes such as endogenous protease and glutamate decarboxylase, and coenzyme pyridoxal phosphate can ultimately affect the content of gamma-aminobutyric acid in brown rice. At present, methods for increasing the content of gamma-aminobutyric acid in germinated brown rice are disclosed, such as a method for enriching gamma-aminobutyric acid in germ of germinated brown rice (patent application No. 200510010375.7), a method for producing germinated brown rice enriched with gamma-aminobutyric acid from a germination nutrient solution (patent application No. 201610106278.6), and the like, but the methods are complex in process, the content of gamma-aminobutyric acid in the produced raw material is still to be increased, and the raw material possibly contains some raw materials which influence food safety. Therefore, a simpler, more productive and safer production method is required in actual production.
Chinese patent application 201610106278.6 discloses a germination nutrient solution and a method for producing gamma-aminobutyric acid-rich germinated brown rice by using the same, wherein the germination nutrient solution takes water as a solvent, and each 1L of water contains 0.5-1.0 g of sodium glutamate, 0.02-0.10 g of rice protein, 1-2 g of alkaline protease preparation and 0.01-0.02 mg of gibberellin. The method comprises the steps of soaking the germinated brown rice in the germination nutrient solution, germinating, and then drying to obtain the germinated brown rice rich in gamma-aminobutyric acid. The germination nutrient solution is low in cost, simple to prepare, safe and efficient, and high in gamma-aminobutyric acid conversion rate, and the gamma-aminobutyric acid content in every 100g of the germinated brown rice is 131.62-215.76 mg, and can be up to 22.1 times of that before germination; the method has simple process and high yield, is suitable for industrial production, and has the germination rate as high as 85 percent; the obtained germinated brown rice has good taste, high nutritive value and wide market prospect. However, the method has the advantages of high cost, use of gibberellin raw materials which are not suitable for being used in the food processing industry, and potential safety hazards of products.
200510010375.7 provides a method for enriching gamma-aminobutyric acid from embryo bud of germinated brown rice. The method comprises the steps of taking giant embryo rice as a raw material, carrying out rice hulling on the raw material to remove glumes to obtain brown rice, germinating the brown rice, taking the embryo to carry out degreasing treatment, soaking the embryo in water at 35-40 ℃ for 4-5 hours, controlling the pH value to be 5-5.5, and carrying out sterilization, drying, puffing and crushing to obtain a product with the gamma-aminobutyric acid content of 900mg/100 g. By the method, the paddy is firstly prepared into the brown rice, then the germination treatment is carried out, and then the enrichment processing is carried out, so that the content of the gamma-aminobutyric acid reaches 500-900 mg/100g or even higher. Although the method can enrich gamma-aminobutyric acid in the brown rice germ, the brown rice germ needs to be separated separately in the actual production process, and the method needs degreasing treatment, and is time-consuming and labor-consuming; in addition, the content of gamma-aminobutyric acid obtained by the patent is not high in view of the content of gamma-aminobutyric acid in the whole raw material of the germinated brown rice, because germs only account for a small part of the germinated brown rice.
Chinese patent 201310200150.2 discloses a composite natural plant extract rice seed initiator, and a preparation method and application thereof. The initiator comprises the following raw materials in percentage by mass: ampelopsis grossedentata extract
47.50-48.50%, tea extract 33.10-34.50%, rosemary extract 14.50-15.00%, vitamin B20.15-0.20%, pantothenic acid 0.15-0.20%, vitamin C0.15-0.20%, folic acid 0.15-0.20%, gamma-aminobutyric acid 0.15-0.20% and aloe gel freeze-dried powder 2.00-3.00%. The invention utilizes the vine tea extract to promote the improvement of cell activity, and considers that when the content of total flavonoids in the vine tea extract exceeds 40 percent and the percentage content ratio of dihydromyricetin to myricetin is 10-12: 1, has strong effect on killing and inhibiting microorganisms. In the invention, the r-aminobutyric acid is an essential important raw material, whether the seeds can germinate or not is considered to be closely related to the content of the r-aminobutyric acid in the embryo, and the initiator contains a certain amount of the r-aminobutyric acid, so that the important guarantee for improving the germination rate and the germination uniformity is provided. The initiator is used for rice seeds and aims to improve the germination rate and the germination uniformity, and the raw materials are selected mainly to solve two most important problems in the initiation process of the rice seeds, namely the composite initiator is used for synergistically promoting the recovery and the improvement of the activity of embryonic cells of the rice seeds and is used for synergistically solving the problem of seed rot caused by microbial propagation in the initiation process of the seeds. The invention not only does not increase the content of the gamma-aminobutyric acid in the embryo, but also needs the gamma-aminobutyric acid as a raw material.
Disclosure of Invention
In order to solve the defects in the prior art, the invention aims to provide the nutrient for improving the content of the gamma-aminobutyric acid in the germinated brown rice and the preparation method thereof, wherein the content of the gamma-aminobutyric acid in the brown rice after the brown rice is soaked in the nutrient solution reaches above 401mg/100g of brown rice.
The invention also aims to provide an application method of the nutrient for improving the content of the gamma-aminobutyric acid in the germinated brown rice.
The nutritional agent for improving the content of gamma-aminobutyric acid in the germinated brown rice comprises the following raw materials in percentage by mass:
in order to further achieve the purpose of the invention, preferably, the raw materials comprise, by mass:
preferably, the vine tea extract contains more than or equal to 40% of total flavonoids by mass, and the mass ratio of dihydromyricetin to myricetin monomer components is 10-12: 1.
preferably, the ampelopsis grossedentata extract is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting Ampelopsis grossedentata dry raw materials by adopting an ethanol extraction method to obtain an Ampelopsis grossedentata extracting solution, and then carrying out a spray drying method to obtain an Ampelopsis grossedentata extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%. Preferably, the ethanol concentration is 90% by volume. The ampelopsis grossedentata adopts ampelopsis grossedentata produced in Guangdong, Guangxi, Hunan, Hubei, Jiangxi and the like, and preferably is ampelopsis grossedentata produced in spring and summer in Zhang Jiajie region of Hunan province.
Preferably, the rosemary extract contains rosmarinic acid in an amount of more than or equal to 5% by mass.
Preferably, the rosemary extract is obtained by the following method: drying stems and leaves of fresh rosemary plants at the temperature of 35-45 ℃ to obtain dried rosemary plant raw materials; preparing rosemary plant extract from dried rosemary plant raw materials by an ethanol extraction method, and performing a spray drying method to obtain a rosemary plant extract; in the ethanol extraction method, the mass ratio of the rosemary plant dry raw material to ethanol is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%. Preferably, the ethanol concentration is 90% by volume.
The preparation method of the nutritional agent for improving the content of the gamma-aminobutyric acid in the germinated brown rice comprises the following steps: weighing the vine tea extract, the rosemary plant extract, the vitamin B2, the sodium glutamate, the vitamin B6, the vitamin C and the folic acid raw materials according to the proportion, uniformly stirring, and packaging or canning the raw materials in a composite aluminum bag to obtain the nutritional agent for improving the content of the gamma-aminobutyric acid in the germinated brown rice.
The application of the nutrient for improving the content of the gamma-aminobutyric acid in the germinated brown rice comprises the following operation steps:
1) preparation of nutrient solution: dissolving the nutrient into water to prepare nutrient solution with mass concentration of 0.1-5%;
2) soaking the brown rice: adding the prepared nutrient solution into the brown rice to be germinated, wherein the nutrient solution is added in an amount of 5-10 cm above the liquid surface after the raw materials are completely covered; soaking for more than 20 hours;
3) germination of brown rice: stirring once every 8-10 hr during germination process to promote brown rice germination until the sprout length is 0.5-1.2 mm;
4) washing and enzyme activity termination: after the germination is finished, cleaning the germination raw materials by using tap water, taking out the germination raw materials after the water washing, inactivating enzymes, and draining water for later use;
5) and (3) drying: drying the germinated brown rice material.
Preferably, the number of times of washing the germinated material with tap water is 2-3 times; the enzyme deactivation is carried out by treating with 75-85 deg.C hot water for more than 10 min.
Preferably, the drying is microwave drying or sun drying of the germinated brown rice raw material. The drying is carried out until the moisture content is less than 13%; and 2) discharging the redundant nutrient solution after soaking for more than 20 hours.
The vitamin B2, the sodium glutamate, the vitamin B6, the vitamin C and the folic acid are all commercially available raw materials.
The brown rice refers to rice produced by various rice varieties (lines) approved by relevant national departments to be used in China, caryopsis of the rice grains are processed after rice hulls with outer protective sheaths are removed, rice grains with intact inner protective sheaths (pericarp, seed coat and nucellar layer), and various rice varieties (lines) comprise japonica rice, indica rice and glutinous rice, and also comprise conventional rice and hybrid rice.
The principle of the invention is as follows: on one hand, the invention improves the enzyme activity and the substrate concentration of the r-aminobutyric acid synthesis so as to promote the synthesis of the r-aminobutyric acid; on the other hand, the invention promotes the balanced germination of the brown rice and improves the germination rate, thereby increasing the synthetic part of the gamma-aminobutyric acid. In order to achieve the purpose, the raw materials of the invention need to be cooperated:
the ampelopsis grossedentata extract: firstly, the vine tea extract can improve the activity of endogenous protease in the brown rice and promote the hydrolysis of protein in the brown rice, so that the content of glutamic acid is improved; the Ampelopsis grossedentata extract can improve the activity of glutamate decarboxylase in the brown rice and promote the effective conversion of the glutamate in the brown rice into gamma-aminobutyric acid; and thirdly, the vine tea extract is dissolved in water to reduce the cell osmotic potential by changing the water potential of the solution, and during the water absorption swelling period of the brown rice, the vine tea extract can be used as an osmotic substance to adjust the uniformity of water absorption of the brown rice, so that the balanced germination is promoted, the germination rate is improved, the sufficient germs required by the production and enrichment of the gamma-aminobutyric acid of the brown rice can be ensured by the uniform and numerous germs, and the content of the gamma-aminobutyric acid in the germinated brown rice is integrally improved.
Rosemary plant extract: the rosemary plant extract is a natural antioxidant, and has the characteristics of high efficiency, oxidation resistance, bacteriostasis, safety, no toxicity, high temperature resistance and the like. In the invention, the rosemary plant extract has the main functions of eliminating active oxygen in brown rice embryo, reducing lipid peroxidation of the brown rice embryo and promoting the improvement of the germination rate of brown rice.
Vitamin B2: also called riboflavin, riboflavin is a component of many important accessory enzymes in the body that can transfer hydrogen during substance metabolism in the body, and it is also a necessary substance for protein, sugar, fatty acid metabolism and energy utilization and composition.
Sodium glutamate: is the sodium salt of glutamic acid, can improve the substrate concentration of glutamate decarboxylase and increase the synthesis of gamma-aminobutyric acid.
Vitamin B6: pyridoxal phosphate is a coenzyme of transaminase and decarboxylase in amino acid metabolism, and can improve glutamate decarboxylase activity, promote glutamate decarboxylation and increase the generation of gamma-aminobutyric acid.
Vitamin C: increasing antibody and enhancing resistance. The antioxidant effect is mainly applied in the invention.
Folic acid: anti-anemia; maintaining normal growth of cells and function of the immune system. In the present invention, the antioxidant effect is mainly applied and the normal growth of cells is maintained.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) calculated by the content of the gamma-aminobutyric acid in the whole germinated brown rice, the content of the gamma-aminobutyric acid in the brown rice soaked in 1.0% of nutrient solution reaches above 401.2mg/100g of brown rice.
(2) The formula for improving the content of the gamma-aminobutyric acid in the germinated brown rice is reasonable in composition, makes full use of the characteristics and effects of the raw materials, and is reasonably combined to enable the raw materials to synergistically exert the best effect.
(3) The processing and the preparation are simple, the use is convenient, and an ideal product is provided for producing the germinated brown rice with high content of the gamma-aminobutyric acid.
(4) The nutrient solution of the invention has no toxicity and low price.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto. The following examples used the following raw materials and equipment and detection methods:
1. the ampelopsis grossedentata extract is prepared by the following steps: drying fresh stems and leaves of vine tea at 40 ℃ to obtain vine tea drying raw materials; the ampelopsis grossedentata extract is obtained by extracting ampelopsis grossedentata dry raw materials by adopting an ethanol extraction method (the mass ratio of the ampelopsis grossedentata dry raw materials to ethanol is 1: 8, and the volume percentage concentration of the ethanol is 90%), and then performing a spray drying method. The vine tea extract contains 55.1 percent of total flavonoids by mass, and the mass percentage of the dihydromyricetin and myricetin monomer components is 10.98: 1.
2. the rosemary plant extract is prepared by the following steps: drying stems and leaves of fresh rosemary plants at the temperature of 40 ℃ to obtain dried rosemary plant raw materials; the preparation method comprises the steps of extracting dry rosemary plant raw materials by using ethanol to obtain rosemary plant extract (in the ethanol extraction method, the mass ratio of the dry rosemary plant raw materials to the ethanol is 1: 10, and the volume percentage concentration of the ethanol is 85%), and performing spray drying to obtain the rosemary plant extract.
3. The vitamin B2 and the sodium glutamate are food grade vitamin B2 and sodium glutamate of 'Xingyingriver brand' produced by Hubei Xingyingriver chemical Limited company; the vitamin B6 is 'xing silver river brand' food grade vitamin B6 produced by Wuhan Hongknkang fine chemical industry limited company; the vitamin C is food-grade vitamin C of Wuhan Dahua brand produced by Wuhan Dahua biotechnology limited; the folic acid is 'xing Ying He brand' food grade folic acid produced by Hubei xing Ying He chemical company Limited.
4. Germination pot (machine): korean, NAGO household intelligent germination machine.
5. The brown rice of hybrid rice is provided by Jinza Rice-seed industry Co., Ltd, Guangdong province.
4. The method for measuring the germination rate comprises the following steps: the germinated brown rice is randomly divided into 3 equal parts, 10.0-15.0 g of brown rice is taken out from each equal part, and the number of the germinated brown rice is counted (the germinated brown rice is obtained when the brown rice germs expose out of the seed coats and the length of the seed coats reaches 0.5-1.0 mm).
The germination rate is the number of germinated brown rice/total brown rice multiplied by 100%
5. Determination of endogenous protease activity: weighing 1g of soaked or germinated coarse cereal raw material, adding 5mL of sodium phosphate buffer solution (0.2mol/L, pH 5.0) into the coarse cereal raw material, grinding the coarse cereal raw material in an ice bath, transferring the coarse cereal raw material into a centrifuge tube, fixing the volume to 9mL by using the buffer solution, centrifuging the centrifuge tube for 10min at the temperature of 4 ℃ and under the condition of 5000r/min, and obtaining supernate, namely the protease crude extract. 2mL of the substrate (0.2% casein) was reacted with 1mL of the enzyme solution at 37 ℃ for 20min, and then 2mL of 10% trichloroacetic acid solution was added to terminate the reaction. Transferring the supernatant into a centrifuge tube, centrifuging for 10min at 8000r/min, collecting supernatant, diluting to 10mL, and measuring absorbance at wavelength of 274 mn. Trichloroacetic acid was added first and then the substrate was added as a blank. The tyrosine content of the supernatant was determined by a standard curve method, defining the hydrolysis of casein at 37 ℃ to yield 1. mu.g of tyrosine per minute as one unit of enzyme activity.
Preparation of a tyrosine standard curve: preparing 100 mu g/mL of tyrosine mother liquor. 1, 2, 3, 4 and 5mL of mother liquor are respectively added with distilled water to supplement to 5mL, and the absorbance is measured at 274nrn to draw a standard curve.
6. Determination of glutamate decarboxylase Activity: weighing 3g of soaked or germinated coarse cereal raw materials, adding 5ml of sodium phosphate buffer solution (0.2mol/L, pH5.7) for grinding in an ice bath, then transferring into a centrifuge tube, fixing the volume to 9ml by using the buffer solution, centrifuging for 10min under the conditions of 4 ℃ and 8000r/min, and obtaining supernatant as crude extract. Reacting 2ml substrate (0.1% glutamic acid, pH5.7) with 1ml enzyme solution at 40 deg.C for 2h, inactivating enzyme at 90 deg.C for 5min, centrifuging at 8000r/min for 20min, and collecting supernatant to determine gamma-aminobutyric acid content. 1 mu mol of gamma-aminobutyric acid generated every 30min is taken as 1 enzyme activity unit.
7. Determination of γ -aminobutyric acid: the method is carried out according to the high performance liquid chromatography for measuring the gamma-aminobutyric acid in the NY/T2890-2016 rice.
Example 1
1) Weighing 48.00 mass percent of vine tea extract, 15.75 mass percent of rosemary extract, 14.30 mass percent of vitamin B2, 10.25 mass percent of sodium glutamate, 4.50 mass percent of vitamin B6, 4.50 mass percent of vitamin C and 2.70 mass percent of folic acid, placing the components into a blending tank, uniformly stirring, and packaging or canning the components in a composite aluminum bag to obtain the nutrient for improving the content of gamma-aminobutyric acid in the germinated brown rice.
2) Preparing 2 germination pots, putting 10 kg of brown rice made of 'Qiuyou 998' rice into the germination pots respectively, pouring 10 kg of nutrient solution with the mass percent of 1.0% made of the nutrient into one germination pot, and pouring 10 kg of purified water into the other germination pot;
3) setting the soaking temperature of 2 germination pots at 25 ℃ and the ventilation quantity at 1.5L/min, and discharging the redundant solution after soaking for 25 hours;
4) after the excessive water in 2 germination pots is discharged, setting the germination temperature at 30 ℃ and the ventilation quantity at 2.0L/min, and stopping germination after 20 hours of germination;
5) opening the germination tank, washing the germinated brown rice with tap water for 3 times, taking out the germinated brown rice after washing, soaking in hot water at 80 deg.C for 10min, and draining; drying the germinated brown rice by microwave drying method until the water content is less than 13.5%.
6) The germination rate, endogenous protease activity, glutamate decarboxylase activity and gamma-aminobutyric acid content of the 'Qiuyou 998' brown rice in 2 germination tanks are detected as shown in table 1:
TABLE 1 change in enzyme activity and gamma-aminobutyric acid content after germination of "Qiuyou 998" brown rice soaked in different solutions
As can be seen from Table 1, when the "Qiuyou 998" brown rice is soaked in a 1.0% nutrient solution for germination, the germination rate, the endogenous protease activity, the glutamate decarboxylase activity and the gamma-aminobutyric acid content of the brown rice are respectively increased to 94.7%, 5.12U, 0.91U and 401.2mg/100g from 87.2%, 1.21U, 0.32U and 175.3mg/100g respectively when the brown rice is soaked in purified water. The main reason is that the nutrient is dissolved in water, so that the water potential of the solution is changed, the cell osmotic potential is reduced, and the nutrient is used as an osmotic substance to adjust the uniformity of water absorbed by the brown rice during the swelling period of the brown rice due to water absorption, so that the balanced germination of the brown rice is promoted, and the germination rate of the brown rice is finally improved; in the germination process, the nutritional agent improves the activity of endogenous protease in the brown rice, promotes the hydrolysis of protein in the brown rice embryo, improves the substrate concentration of glutamate decarboxylase, simultaneously improves the activity of the glutamate decarboxylase, and promotes the glutamic acid in the brown rice embryo to be effectively converted into gamma-aminobutyric acid, thereby improving the content of the gamma-aminobutyric acid in the germinated brown rice.
It should be noted that, compared with the method for enriching gamma-aminobutyric acid in the germ of the germinated brown rice provided by the invention patent 200510010375.7 in China, 200510010375.7 detects the content of gamma-aminobutyric acid in the sprout, and the invention detects the content of gamma-aminobutyric acid in the whole germinated brown rice.
Example 2
1) Weighing 47.50 mass percent of vine tea extract, 16.25 mass percent of rosemary extract, 14.50 mass percent of vitamin B2, 10.05 mass percent of sodium glutamate, 4.25 mass percent of vitamin B6, 4.75 mass percent of vitamin C and 2.70 mass percent of folic acid, placing the components in a blending tank, uniformly stirring, and packaging or canning the components in a composite aluminum bag to obtain the nutrient for improving the content of gamma-aminobutyric acid in the germinated brown rice.
2) Preparing 2 germination pots, putting 5 kg of brown rice made of Tianyou 268 rice in the germination pots, pouring 5 kg of nutrient solution with the mass percent of 0.5% and made of the nutrient, and pouring 5 kg of purified water into the germination pots;
3) setting the soaking temperature of 2 germination pots at 27 ℃ and the ventilation volume at 1.2L/min, and discharging the excess water after soaking for 20 hours;
4) after the excessive water in 2 germination pots is discharged, setting the germination temperature at 28 ℃ and the ventilation volume at 1.5L/min, and stopping germination after germination for 24 hours;
5) opening the germination tank, washing the germinated brown rice with tap water for 2 times, taking out the germinated brown rice after washing, soaking in hot water at 75 deg.C for 10min, and draining; drying the germinated brown rice by microwave drying method until the water content is less than 13.5%.
6) The germination rate, endogenous protease activity, glutamate decarboxylase activity and gamma-aminobutyric acid content of the 'Tianyou 268' brown rice in 2 germination pots are detected as shown in Table 2:
TABLE 2 change of enzyme activity and gamma-aminobutyric acid content after soaking of "Tianyou 268" brown rice in different solutions
As shown in Table 2, when "Tianyou 268" was soaked in 1.5% vine tea extract for germination, the germination percentage, endogenous protease activity, glutamate decarboxylase activity and gamma-aminobutyric acid content of brown rice were respectively increased to 95.7%, 4.78U, 1.02U and 451.2mg/100g from 87.2%, 1.54U, 0.39U and 187.2mg/100g of purified water when soaked. The main reason is that the nutrient is dissolved in water, so that the water potential of the solution is changed, the cell osmotic potential is reduced, and the nutrient is used as an osmotic substance to adjust the uniformity of water absorbed by the brown rice during the swelling period of the brown rice due to water absorption, so that the balanced germination of the brown rice is promoted, and the germination rate of the brown rice is finally improved; in the germination process, the nutritional agent improves the activity of endogenous protease in the brown rice, promotes the hydrolysis of protein in the brown rice embryo, improves the substrate concentration of glutamate decarboxylase, simultaneously improves the activity of the glutamate decarboxylase, and promotes the glutamic acid in the brown rice embryo to be effectively converted into gamma-aminobutyric acid, thereby improving the content of the gamma-aminobutyric acid in the germinated brown rice.
Example 3
1) Weighing 47.50 mass percent of vine tea extract, 16.75 mass percent of rosemary extract, 14.00 mass percent of vitamin B2, 10.25 mass percent of sodium glutamate, 4.05 mass percent of vitamin B6, 4.45 mass percent of vitamin C and 3.00 mass percent of folic acid, placing the components in a blending tank, uniformly stirring, and packaging or canning the components in a composite aluminum bag to obtain the nutrient for improving the content of gamma-aminobutyric acid in the germinated brown rice.
2) Preparing 2 germination pots, putting 5 kg of brown rice made of Boyou 998 rice in the germination pots, pouring 5 kg of nutrient solution with the mass percent of 1.25% and prepared from the nutrient in one pot, and pouring 5 kg of purified water in the other pot;
3) setting the soaking temperature of 2 germination pots at 35 ℃ and the ventilation quantity at 2.0L/min, and discharging the excess water after soaking for 25 hours;
4) after the excessive water in 2 germination pots is discharged, setting the germination temperature at 30 ℃ and the ventilation volume at 2.0L/min, and stopping germination after germination for 25 hours;
5) opening the germination tank, washing the germinated brown rice with tap water for 3 times, taking out the germinated brown rice after washing, soaking in hot water at 85 deg.C for 8min, and draining; drying the germinated brown rice by microwave drying method until the water content is less than 13.0%.
6) The germination rate, endogenous protease activity, glutamate decarboxylase activity and gamma-aminobutyric acid content of the 'Boyou 998' brown rice in 2 germination tanks are detected as shown in the table 3:
TABLE 3 change of enzyme activity and gamma-aminobutyric acid content after soaking of "Boyou 998" brown rice in different solutions for germination
As shown in Table 3, when "Boyou 998" was soaked in 2.0% Ampelopsis grossedentata extract for germination, the germination percentage, endogenous protease activity, glutamate decarboxylase activity and gamma-aminobutyric acid content of brown rice were respectively increased from 87.5%, 1.65U, 0.41U and 191.3mg/100g in pure water to 94.7%, 4.91U, 1.13U and 471.5mg/100g, respectively. The main reason is that the nutrient is dissolved in water, so that the water potential of the solution is changed, the cell osmotic potential is reduced, and the nutrient is used as an osmotic substance to adjust the uniformity of water absorbed by the brown rice during the swelling period of the brown rice due to water absorption, so that the balanced germination of the brown rice is promoted, and the germination rate of the brown rice is finally improved; in the germination process, the nutritional agent improves the activity of endogenous protease in the brown rice, promotes the hydrolysis of protein in the brown rice embryo, improves the substrate concentration of glutamate decarboxylase, simultaneously improves the activity of the glutamate decarboxylase, and promotes the glutamic acid in the brown rice embryo to be effectively converted into gamma-aminobutyric acid, thereby improving the content of the gamma-aminobutyric acid in the germinated brown rice.
The embodiments of the present invention are not limited to the above-described embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and they are included in the scope of the present invention.
Claims (6)
1. The nutritional agent for improving the content of gamma-aminobutyric acid in the germinated brown rice is characterized by comprising the following raw materials in percentage by mass:
47.50-48.50% of vine tea extract
Rosemary extract 15.50-16.00%
Vitamin B214.25-14.30%
10.00 to 15.00 percent of sodium glutamate
Vitamin B64.15-4.9%
4.15 to 4.9 percent of vitamin C
2.3 to 3.40 percent of folic acid
The vine tea extract contains more than or equal to 40% of total flavonoids by mass, and the mass ratio of dihydromyricetin to myricetin monomer components in the vine tea extract is 10-12: 1; the ampelopsis grossedentata extract is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting Ampelopsis grossedentata dry raw materials by adopting an ethanol extraction method to obtain an Ampelopsis grossedentata extracting solution, and then carrying out a spray drying method to obtain an Ampelopsis grossedentata extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%;
in the rosemary extract, the mass percentage content is more than or equal to 5 percent in terms of rosmarinic acid; the rosemary extract is obtained by the following method: drying stems and leaves of fresh rosemary plants at the temperature of 35-45 ℃ to obtain dried rosemary plant raw materials; preparing rosemary plant extract from dried rosemary plant raw materials by an ethanol extraction method, and performing a spray drying method to obtain a rosemary plant extract; in the ethanol extraction method, the mass ratio of the rosemary plant dry raw material to ethanol is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%.
2. The nutritional agent for increasing the content of gamma-aminobutyric acid in the germinated brown rice as claimed in claim 1, wherein the nutritional agent comprises the following raw materials by mass percent:
48.00 percent of vine tea extract
Rosemary extract 15.75%
Vitamin B214.30%
Sodium glutamate 10.25%
Vitamin B64.50%
4.50 percent of vitamin C
And 2.70% of folic acid.
3. The method for preparing the nutritional agent for improving the content of the gamma-aminobutyric acid in the germinated brown rice as claimed in claim 1, wherein the raw materials of the vine tea extract, the rosemary plant extract, the vitamin B2, the sodium glutamate, the vitamin B6, the vitamin C and the folic acid are weighed according to the proportion, are uniformly stirred, and are packaged in a composite aluminum bag or canned to obtain the nutritional agent for improving the content of the gamma-aminobutyric acid in the germinated brown rice.
4. The use of the nutritional agent for increasing the content of gamma-aminobutyric acid in germinated brown rice as claimed in claim 1, comprising the steps of:
1) preparation of nutrient solution: dissolving the nutrient into water to prepare nutrient solution with mass concentration of 0.1-5%;
2) soaking the brown rice: adding the prepared nutrient solution into the brown rice to be germinated, wherein the nutrient solution is added in an amount of 5-10 cm above the liquid surface after the raw materials are completely covered; soaking for more than 20 hours;
3) germination of brown rice: stirring once every 8-10 hr during germination process to promote brown rice germination until the sprout length is 0.5-1.2 mm;
4) washing and enzyme activity termination: after the germination is finished, cleaning the germination raw materials by using tap water, taking out the germination raw materials after the water washing, inactivating enzymes, and draining water for later use;
5) and (3) drying: drying the germinated brown rice material.
5. The use of the nutritional agent for increasing the content of gamma-aminobutyric acid in the germinated brown rice according to claim 4, wherein the number of times of washing the germinated material with tap water is 2 to 3; the enzyme deactivation is carried out by treating with 75-85 deg.C hot water for more than 10 min.
6. The use of the nutritional agent for increasing the content of gamma-aminobutyric acid in the germinated brown rice according to claim 4, wherein the drying is microwave drying or sun drying of the germinated brown rice;
the drying is carried out until the moisture content is less than 13%; and 2) draining the redundant nutrient solution after soaking for more than 20 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710716651.4A CN107417400B (en) | 2017-08-21 | 2017-08-21 | Nutrient for improving content of gamma-aminobutyric acid in germinated brown rice and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710716651.4A CN107417400B (en) | 2017-08-21 | 2017-08-21 | Nutrient for improving content of gamma-aminobutyric acid in germinated brown rice and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107417400A CN107417400A (en) | 2017-12-01 |
| CN107417400B true CN107417400B (en) | 2021-04-06 |
Family
ID=60434162
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710716651.4A Active CN107417400B (en) | 2017-08-21 | 2017-08-21 | Nutrient for improving content of gamma-aminobutyric acid in germinated brown rice and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107417400B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109430725B (en) * | 2018-12-28 | 2022-02-18 | 河北省农林科学院谷子研究所 | Industrial production method of germinated millet steamed bread |
| CN109430852B (en) * | 2018-12-28 | 2022-02-18 | 河北省农林科学院谷子研究所 | Preparation method of germinated maize eight-treasure porridge with stomach nourishing function |
| CN109430025B (en) * | 2018-12-28 | 2022-02-01 | 河北省农林科学院谷子研究所 | Method for industrially producing germinated millet |
| CN109430724B (en) * | 2018-12-28 | 2022-02-18 | 河北省农林科学院谷子研究所 | Preparation method of germinated millet nutrition powder |
| CN109430401B (en) * | 2018-12-28 | 2022-02-18 | 河北省农林科学院谷子研究所 | Preparation method of germinated millet yoghourt by mixed fermentation |
| CN109673954B (en) * | 2018-12-28 | 2022-02-18 | 河北省农林科学院谷子研究所 | Industrial production method of germinated millet sandbags |
| CN112119896A (en) * | 2020-09-27 | 2020-12-25 | 湖南米米梦工场科技股份有限公司 | Germination method of brown rice |
| CN112715852A (en) * | 2021-01-04 | 2021-04-30 | 玛士撒拉无锡医疗科技有限公司 | Preparation process of germinated brown rice |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250744B (en) * | 2013-05-24 | 2014-04-30 | 广东省农业科学院水稻研究所 | Composite natural plant extract rice seed initiator as well as preparation method and application thereof |
| CN105693294B (en) * | 2016-02-26 | 2019-08-09 | 中南林业科技大学 | Germination nutrient solution and the method for being rich in γ-aminobutyric acid sprouted unpolished rice with its production |
-
2017
- 2017-08-21 CN CN201710716651.4A patent/CN107417400B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107417400A (en) | 2017-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107417400B (en) | Nutrient for improving content of gamma-aminobutyric acid in germinated brown rice and preparation method and application thereof | |
| EP2322042B1 (en) | Method for preparing raw material for functional foods from barley or wheat seeds | |
| CN107439865B (en) | Compound gel and grain suspension beverage and preparation method thereof | |
| CN107988011B (en) | Black glutinous rice wine and preparation method thereof | |
| CN107410847A (en) | A kind of high GABA rice product and its processing method | |
| CN105062781B (en) | A kind of preparation method of floral type litchi spirit | |
| US9161495B2 (en) | Method for preparing raw material for functional foods from barley or wheat seeds | |
| CN107348070A (en) | Processing method rich in the full grain germination rice tea of gamma aminobutyric acid bitter buckwheat | |
| CN103710188B (en) | Method for preparing health-care purple sweet potato beer | |
| CN104664330A (en) | Dry edible-fungi food and processing method thereof | |
| CN107136259B (en) | Production method of wheat malt tea rich in gamma-aminobutyric acid | |
| CN102210434A (en) | Mango powder and preparation method thereof | |
| CN102948464B (en) | Production method of nutritional rice with good cooking performance and storability | |
| CN111700213A (en) | Rice processing technology | |
| CN107522520B (en) | Nutrient for improving gamma-aminobutyric acid content in germinated bean raw material and preparation method and application thereof | |
| CN107347299B (en) | Method for improving content of gamma-aminobutyric acid in germinated coarse grain raw material by using vine tea extract | |
| CN107512937B (en) | Nutritional agent for improving content of gamma-aminobutyric acid in germinated wheat raw materials and preparation method and application thereof | |
| CN108041220A (en) | A kind of weight reducing health protection tea and preparation method thereof | |
| CN103289865A (en) | A processing method for nostoc sphaeroides rice wine | |
| CN113170832A (en) | Method for reducing content of volatile components in pea protein isolate | |
| CN107865305A (en) | A kind of nutrient health, the sprouted unpolished rice steamed sponge cake to look good, smell good and taste good and preparation method thereof | |
| CN113142351A (en) | Health vine tea and its production process | |
| CN104247911A (en) | Germ rice and preparation method thereof | |
| KR101133898B1 (en) | Alcohol detoxification composition used Matured dried persimmon | |
| CN108342293B (en) | Production method of powdery schisandra fruit vinegar |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |