CN107417400A - Improve the nutritional agents of gamma aminobutyric acid content and preparation method and application in sprouted unpolished rice - Google Patents
Improve the nutritional agents of gamma aminobutyric acid content and preparation method and application in sprouted unpolished rice Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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Abstract
The invention discloses improve the nutritional agents of alpha-aminobutyric acid content and preparation method and application in sprouted unpolished rice；Below by mass percentage, its raw material composition is：Ampelopsis grossedentata extrat 47.50~48.50%, Rosmarinus officinalis extract 15.50~16.00%, vitamin B2 14.25~14.30%, sodium glutamate 10.00~15.00%, vitamin B6 4.15~4.9%, vitamin C 4.15~4.9, folic acid 2.3~3.40%.With the cubage of gamma aminobutyric acid in whole sprouted unpolished rice, brown rice of the present invention soaks gamma aminobutyric acid content in nutrition agent solution and reached more than 401.2mg/100g brown rice.Nutrient solution of the present invention is low without any toxicity, price.
Description
Technical field
The present invention relates to γ-aminobutyric acid, more particularly to a kind of nutrition for improving alpha-aminobutyric acid content in sprouted unpolished rice
Agent and preparation method and application.
Background technology
γ-aminobutyric acid is a kind of a kind of active component being widely present in animals and plants.Modern medicine proves, γ-ammonia
Base butyric acid has calm nerve, antianxiety, reduces blood pressure, and reduces blood ammonia, improves brain vigor, promotes alcohol metabolism, prevents skin
Aging, eliminate body odor, improve lipid-metabolism, preventing the functions such as artery sclerosis, high-efficient fat reducing.People are often improved with sprouted unpolished rice
The content of γ-aminobutyric acid in staple food, to reach the more γ-aminobutyric acids of intake, the purpose of physical fitness.
γ-aminobutyric acid belongs to nonprotein amino acid, and the protein in brown rice embryo is in the presence of endogenous protease
Amino acid is hydrolyzed into, glutamic acid therein generates γ-aminobutyric acid in the presence of glutamate decarboxylase, and its coenzyme is phosphoric acid
Pyridoxal.In addition, γ-aminobutyric acid synthesising part is primarily present in plumule.Therefore, it is every to influence to close with γ-aminobutyric acid
Into, relevant substrate such as glutamic acid, enzyme such as endogenous protease and glutamate decarboxylase and the coenzyme phosphopyridoxal pyridoxal phosphate of metabolism all
The content of γ-aminobutyric acid in brown rice can finally be influenceed.At present, γ-aminobutyric acid in many raising sprouted unpolished rices is disclosed to contain
The method of amount, such as " method of enriching gamma-aminobutyric aid from sprouting brown-rice germ " (number of patent application 200510010375.7), " hair
Bud nutrient solution and method for being rich in γ-aminobutyric acid sprouted unpolished rice with its production " (number of patent application 201610106278.6) etc.,
But these methods one are technics comparing complexity, second, alpha-aminobutyric acid content need to be improved in the raw material of production, third, wherein
It is possible to the raw material containing some influence food securities.Therefore, needed in actual production it is simpler, more fruitful and
Safer production method.
Chinese invention patent application 201610106278.6 discloses germination nutrient solution and is rich in gamma-amino fourth with its production
The method of sour sprouted unpolished rice, the germination nutrient solution contain 0.5~1.0g of sodium glutamate, rice using water as solvent in every 1L water
0.01~0.02mg of 0.02~0.10g of albumen, 1~2g of basic protein enzyme preparation and gibberellin.Methods described is by sprouted unpolished rice
By immersion in the germination nutrient solution, germination, then dry, obtain the sprouted unpolished rice rich in γ-aminobutyric acid.Present invention hair
Bud nutrient solution cost is low, prepares simple, safe efficient, γ-aminobutyric acid high conversion rate, contains in sprouted unpolished rice obtained by per 100g
γ-aminobutyric acid is up to 131.62~215.76mg, reaches as high as 22.1 times before germination；The inventive method technique is simple, production
Amount is big, is suitable for industrialized production, germination percentage may be up to 85%；Gained sprouted unpolished rice mouthfeel is good, and nutritive value is high, has wide
Market prospects.But be present cost height, used " gibberellin " raw material that should not be used in food-processing industry in the invention, produce
Potential potential safety hazard be present in product.
200510010375.7 provide a kind of method of enriching gamma-aminobutyric aid from sprouting brown-rice germ.With Characters of Giant Embryo Rice
Paddy is raw material, raw material first pass through rice huller paddy processing slough it is coetonium obtain brown rice, brown rice is germinateed, takes plumule to carry out at degreasing
Reason, then place it in 35-40 DEG C of water and soak 4-5 hours and control ph in 5-5.5, then by sterilizing, it is dry, expanded,
Crushing obtains product of the alpha-aminobutyric acid content in 500-900mg/100g.By the method for the present invention, paddy is first made rough
Rice, then germination treatment is carried out, then Beneficiation is carried out, alpha-aminobutyric acid content is reached 500-900mg/100g even more
It is high.Although the invention can be enriched with γ-aminobutyric acid in brown rice germ, by brown rice in actual production process
Plumule is separately separated out, and needs ungrease treatment, time-consuming bothersome；In addition from the gamma-amino in sprouted unpolished rice entirety raw material
From the point of view of butyric acid content, the alpha-aminobutyric acid content obtained by the patent of invention is not high because plumule only account for sprouted unpolished rice it is one small
Part.
Chinese invention patent 201310200150.2 discloses a kind of composite natral plant extract rice paddy seed initiator
And its preparation method and application.The initiator is made up of following mass percent meter raw material：Ampelopsis grossedentata extrat
47.50~48.50%, tea extract 33.10~34.50%, Rosmarinus officinalis extract 14.50~15.00%, dimension
Raw plain B20.15~0.20%, pantothenic acid 0.15~0.20%, vitamin C 0.15~0.20%, folic acid 0.15~0.20%, γ-
Aminobutyric acid 0.15~0.20% and Aloeglue 2.00~3.00%.Wherein, the chief active in Ampelopsis grossedentata extrat into
It is divided into flavanone kind composition and flavone compound, the invention promotes cell viability to improve using Ampelopsis grossedentata extrat, and
Think when general flavone content is more than 40% and the percentage composition ratio of dihydromyricetin and myricetin is 10- in Ampelopsis grossedentata extrat
12：When between 1, killing and suppression strong effect to microorganism.R- aminobutyric acids are a kind of essential weights in the invention
Want constitutive material, it is believed that can seed germinate closely related with the content of r- aminobutyric acids in embryo, and initiator contains certain r-
Aminobutyric acid is the regularity important guarantee for improving germination percentage and germination.But the initiator is to be used for rice paddy seed, it is therefore intended that
Germination percentage and the regularity of germination are improved, selects above-mentioned raw materials to essentially consist in two in rice paddy seed elicitation procedure to be solved most
The problem of important, first, composite initiator will cooperate with the recovery and raising for promoting rice paddy seed blastocyte vigor, second, to cooperate with
Microbial reproduction is to the rotten problem caused by seed during solving seed priming.The invention does not increase r- amino in embryo not only
Butyric acid content, it is also necessary to be used as raw material by the use of r- aminobutyric acids.
The content of the invention
In order to solve above-mentioned the shortcomings of the prior art, it is an object of the invention to provide one kind to improve germination
Nutritional agents of alpha-aminobutyric acid content and preparation method thereof in brown rice, brown rice γ-ammonia after immersion treatment in nutrition agent solution
Base butyric acid content reaches more than 401mg/100g brown rice.
Another object of the present invention is in the application side of the nutritional agents of alpha-aminobutyric acid content in the raising sprouted unpolished rice is provided
Method.
The nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice supports agent, by mass percentage, its raw material composition
For：
Further to realize the object of the invention, it is preferable that by mass percentage, its raw material composition is：
Preferably, in the Ampelopsis grossedentata extrat, in terms of general flavone, weight/mass percentage composition >=40%, and wherein dihydromyricetin
The mass ratio of element and myricetin monomer component is 10~12：1.
Preferably, the Ampelopsis grossedentata extrat is to be prepared according to the following steps：Using the fresh cauline leaf of vine tea, in temperature 35
Vine tea dry feedstock is obtained after being dried under the conditions of~45 DEG C；Vine tea dry feedstock is obtained into vine tea extraction using ethanol extraction method
Liquid, then spray-dried method obtain Ampelopsis grossedentata extrat；The mass ratio of vine tea dry feedstock and ethanol is in the ethanol extraction method
1：10~12, the concentration of volume percent of ethanol is 85~95%.Preferred alcohol concentration of volume percent 90%.The vine tea is adopted
With the vine tea of the real estates such as Guangdong, Guangxi, Hunan, Hubei, Jiangxi, the vine tea that preferably Hunan Zhangjiajie spring and summer season produces.
Preferably, in the Rosmarinus officinalis extract, in terms of Rosmarinic acid, weight/mass percentage composition >=5%.
Preferably, the Rosmarinus officinalis extract extract is to obtain by the following method：By fresh rosemary plant stem and leaf
Rosemary plant dry feedstock is obtained after being dried under the conditions of 35~45 DEG C of temperature；Rosemary plant dry feedstock is used into ethanol
Extraction method obtains rosemary plant extract solution, then spray-dried method obtains rosemary；The ethanol extraction method
The mass ratio of middle rosemary plant dry feedstock and ethanol is 1：10~12, the concentration of volume percent of ethanol is 85~95%.
Preferred alcohol concentration of volume percent 90%.
The described nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice supports the preparation method of agent：Weighed by proportioning
Ampelopsis grossedentata extrat, rosemary, vitamin B2, sodium glutamate, vitamin B6, vitamin C and folic acid raw material, stirring
Uniformly, after compositing aluminium bag is packed or be canned, a kind of nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice is produced.
The described nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice supports the application of agent, including following operation step
Suddenly：
1) preparation of nutrition agent solution：The nutritional agents is dissolved into water, prepares the battalion that mass concentration is 0.1%-5%
Support agent solution；
2) immersion of brown rice：The nutrition agent solution of preparation is added in the brown rice for needing to germinate, the addition of nutrition agent solution
Measure still to expose liquid level 5-10cm after the full raw material of all lids；Immersion more than 20 hours；
3) germination of brown rice：In germination process, stirred once per 8-10 hours, promote brown rice germination, to the bud length grown
Stop for 0.5-1.2mm；
4) cleaning, enzymatic activity terminate：After germination terminates, germination raw material is cleaned with running water, takes germination raw material after washing
Go out, enzyme deactivation, water is drained stand-by；
5) dry：By the brown rice raw material drying after germination.
Preferably, the number that germination raw material is cleaned with running water is 2-3 times；Described enzyme deactivation is to use 75-85 DEG C
More than the hot water treatment 10min of temperature.
Preferably, described drying is by the brown rice raw material microwave or sun dehydration after germination.Described drying is dry
Untill moisture is less than content 13%；Step 2) also includes discharging unnecessary nutrition agent solution after soaking more than 20 hours.
The vitamin B2, sodium glutamate, vitamin B6, vitamin C and folic acid are marketable material.
The brown rice refers to the paddy produced through the various rice varieties (being) that national correlation department approval uses in China,
The processed caryopsis sloughed after outer protection cortex rice husk of paddy, the intact rice seed of interior protection cortex (pericarp, kind skin, megarchidium layer)
Grain, various rice varieties (being) include japonica rice, long-grained nonglutinous rice and glutinous rice, also including conventional rice and hybrid rice.
The present invention principle be：One aspect of the present invention improves the enzymatic activity and concentration of substrate of r- aminobutyric acids synthesis,
Promote the synthesis of r- aminobutyric acids with this；On the other hand the present invention promotes the balanced germination of brown rice, improves germination percentage, is increased with this
R- aminobutyric acid synthesising parts.To achieve the above object, coordinated is needed between raw material of the present invention：
Ampelopsis grossedentata extrat：First, Ampelopsis grossedentata extrat can improve the vigor of endogenous protease in brown rice, promote egg in brown rice
The hydrolysis of white matter, content of glutamic acid is improved with this；Second, Ampelopsis grossedentata extrat can improve the vigor of brown rice Glutamic Acid decarboxylase, promote
Enter brown rice Glutamic Acid and be effectively converted into γ-aminobutyric acid；Third, the Ampelopsis grossedentata extrat flow of water soluble in water by changing solution
To reduce Premeabilisation of cells gesture, during brown rice absorbs water swollen, Ampelopsis grossedentata extrat can adjust brown rice as an infiltration matter and absorb
The uniformity of moisture, so as to promote balanced germination and improve germination percentage, neat and consistent and large number of plumule can ensure
The sufficient plumule that brown rice needs in production and enriching gamma-aminobutyric, so as to improve gamma-amino fourth in sprouted unpolished rice on the whole
The content of acid.
Rosemary：Rosemary is a kind of natural, has high-efficiency antioxidant, suppression
The characteristic such as bacterium, safe and non-toxic, high temperature resistant.In the present invention, the main function of rosemary is removed in brown rice embryo
The ability of active oxygen, the lipid peroxidation for mitigating brown rice embryo, promote the raising of brown rice germination rate.
Vitamin B2：Riboflavin is called, core allusion quotation element is the constituent of internal many important coenzyme classes, and these enzymes can be in body
Hydrogen is transmitted in interior substance metabolism process, thing necessary to it or protein, sugar, fatty acid metabolism and energy utilization and composition
Matter.
Sodium glutamate：For the sodium salt of glutamic acid, the concentration of substrate of glutamate decarboxylase can be improved, increases γ-aminobutyric acid
Synthesis.
Vitamin B6：Also known as phosphopyridoxal pyridoxal phosphate, be transaminase in amino acid metabolism and decarboxylase coenzyme, paddy can be improved
Propylhomoserin decarboxylase, promote glutamic acid decarboxylase, increase the generation of γ-aminobutyric acid.
Vitamin C：Increase antibody, resistance building.Mainly apply its antioxidation in the present invention.
Folic acid：Anti-anemia action；Safeguard the normal growth of cell and the function of immune system.Mainly apply it in the present invention
Antioxidation and the normal growth for safeguarding cell.
The present invention compared with the prior art, has the following advantages and beneficial effect：
(1) with the cubage of γ-aminobutyric acid in whole sprouted unpolished rice, brown rice of the present invention is in 1.0% nutrition agent solution
Middle immersion alpha-aminobutyric acid content reaches more than 401.2mg/100g brown rice.
(2) present invention is formed rationally for improving the formula of alpha-aminobutyric acid content in sprouted unpolished rice, is taken full advantage of each
The characteristic and effect that raw material is each provided with, rationally assembling makes its collaboration play best effects.
(3) processing and fabricating is simple, easy to use, and one kind is provided to produce the γ-aminobutyric acid sprouted unpolished rice of high content
Preferable product.
(4) nutrient solution of the present invention is low without any toxicity, price.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Each raw material used in following examples and equipment and detection method are as follows：
1st, Ampelopsis grossedentata extrat is to be prepared according to the following steps：Using the fresh cauline leaf of vine tea, under the conditions of 40 DEG C of temperature
Vine tea dry feedstock is obtained after drying；Vine tea dry feedstock is used into the ethanol extraction method (quality of vine tea dry feedstock and ethanol
Ratio is 1：8, the concentration of volume percent of ethanol is 90%) to obtain vine tea extract solution, then spray-dried method obtains vine tea and carried
Take thing.In gained Ampelopsis grossedentata extrat, in terms of general flavone, weight/mass percentage composition 55.1%, and wherein dihydromyricetin and myricetin
The weight/mass percentage composition ratio of monomer component is 10.98：1.
2nd, rosemary is to be prepared according to the following steps：By fresh rosemary plant stem and leaf in temperature 40
Rosemary plant dry feedstock is obtained after being dried under the conditions of DEG C；Rosemary plant dry feedstock is confused using ethanol extraction method
Repeatedly (mass ratio of rosemary plant dry feedstock and ethanol is 1 to fragrant plant extraction liquid in ethanol extraction method：10, the body of ethanol
85%) product percent concentration is, then spray-dried method obtains rosemary.
3rd, the vitamin B2 and sodium glutamate are " emerging milky way board " food of the emerging milky way Chemical Co., Ltd. production in Hubei
Level vitamin B2 and sodium glutamate；The vitamin B6 is " the emerging milky way board " of Wuhan Hong Xinkang Fine Chemical Co., Ltd production
Food-grade vitamin B6；The vitamin C is " the big magnificent board in Wuhan " food-grade of Wuhan great Hua bio tech ltd production
Vitamin C；The folic acid is " emerging milky way board " food-grade folic acid of the emerging milky way Chemical Co., Ltd. production in Hubei
4th, germinate tank (machine)：South Korea produces, NAGO domestic intelligent sprouting machines.
5th, hybrid rice brown rice is all provided by Golden Rice Seeds Co., Ltd. of Guangdong.
4th, the assay method of germination percentage is：Sprouted unpolished rice is randomly divided into 3 equal portions, from each equal portions take out 10.0~
15.0g brown rice, statistics wherein sprouted unpolished rice number (brown rice germ expose kind of skin up to 0.5~1.0mm length be sprouted unpolished rice).
Germination percentage=sprouted unpolished rice number/total brown rice number × 100%
5th, the measure of endogenous protease activities：Weigh 1g and weigh five cereals raw material after immersion or rudiment, add 5mL phosphoric acid
Sodium salt buffer solution (0.2mol/L, pH 5.0) is ground in ice bath, is then transferred in centrifuge tube and is settled to 9mL with buffer solution, 4
DEG C, centrifuge 10min under the conditions of 5000r/min, supernatant is albumen enzyme extract.Take 2mL substrates (0.2% casein) with
1mL enzyme liquids react 20min at 37 DEG C, then add 2mL10% solution of trichloroacetic acid terminating reaction.It is transferred in centrifuge tube
8000r/min centrifuges 10min, takes supernatant to be settled to 10mL, its absorbance is surveyed at 274mn wavelength.First plus after trichloroacetic acid
Add substrate as blank test.Tyrosine content in supernatant is determined with calibration curve method, is defined on hydrolysis per minute at 37 DEG C
The tyrosine that casein produces 1 μ g is an enzyme activity unit.
The preparation of tyrosine standard curve：Prepare 100 μ g/mL tyrosine mother liquors.Take 1 respectively, 2,3,4,5mL mother liquors add steaming
Slip water and add to 5mL, absorbance is determined at 274nrn, draw standard curve.
6th, the measure of glutamate decarboxylase activity：Weigh immersion or rudiment after five cereals raw material 3g, add 5ml sodium ascorbyl phosphates
Ground in buffer solution (0.2mol/L, pH5.7) ice bath, be then transferred in centrifuge tube and be settled to 9ml with buffer solution, 4 DEG C,
10min is centrifuged under the conditions of 8000r/min, supernatant is crude extract.2ml substrates (0.1% glutamic acid, pH5.7) and 1ml enzyme liquids
2h is reacted in 40 DEG C, then 90 DEG C of enzyme deactivation 5min, 20min is centrifuged in 8000r/min, take supernatant measure γ-aminobutyric acid to contain
Amount.1 μm of ol γ-aminobutyric acid is generated using every 30min and is used as 1 enzyme activity unit.
7th, the measure of γ-aminobutyric acid：According to " the efficient liquid of measure of γ-aminobutyric acid in NY/T 2890-2016 rice
Phase chromatography " is carried out.
Embodiment 1
1) weigh the Ampelopsis grossedentata extrat of mass percent 48.00%, mass percent 15.75% Rosmarinus officinalis extract,
The vitamin B2 of mass percent 14.30%, the sodium glutamate of mass percent 10.25%, the dimension of mass percent 4.50%
Raw plain B6, the vitamin C of mass percent 4.50% and the folic acid of mass percent 2.70% are placed in blend tank, and stirring is equal
Even, the nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice is packed or produced after canned to compositing aluminium bag.
2) prepare 2 germination tanks, respectively take 10 kilograms by " brown rice is placed in germination tank made of the paddy of Qiu You 998 ", one
Pour into 10 kilograms made of above-mentioned nutritional agents mass percent be 1.0% nutrition agent solutions, another pour into 10 kilograms it is pure
Water purification；
3) soaking temperature for setting 2 germination tanks is 25 DEG C, throughput is 1.5L/min, and immersion will be more after 25 hours
Remaining solution discharge；
4) after water discharge unnecessary in 2 germination tanks, while 30 DEG C of germination temperature, throughput 2.0L/min are set, sent out
Bud stops germination after 20 hours；
5) germination tank is opened, sprouted unpolished rice is cleaned 3 times with running water, takes out sprouted unpolished rice after washing, then with 80 DEG C of temperature
The hot-water soak 10min of degree, water is drained；Brown rice after germination is less than content with microwave drying mode drying to moisture
Untill 13.5%.
6) " germination percentage, endogenous protease activities, the glutamate decarboxylase of the brown rice of Qiu You 998 " in 2 germination tanks is detected
The content such as table 1 of activity and γ-aminobutyric acid：
" enzymatic activity changes the immersion of the different solutions of table 1 with alpha-aminobutyric acid content after the brown rice germinations of Qiu You 998 "
As can be seen from Table 1, " brown rice of Qiu You 998 " soaks germination in 1.0% nutrition agent solution, the germination percentage of brown rice,
When endogenous protease activities, glutamate decarboxylase activity and alpha-aminobutyric acid content are soaked by pure water respectively 87.2%,
1.21U, 0.32U and 175.3mg/100g are respectively increased to 94.7%, 5.12U, 0.91U and 401.2mg/100g.It is main former
Because being that nutritional agents is soluble in water, the flow of water of solution is changed, reduces Premeabilisation of cells gesture, during brown rice absorbs water swollen, battalion
The uniformity that agent absorbs moisture as an infiltration matter regulation brown rice is supported, it is final to improve so as to promote the balanced germination of brown rice
The germination percentage of brown rice；In germination process, nutritional agents improves endogenous protease activities in brown rice, promotes protein in embryo
Hydrolysis, improve glutamate decarboxylase concentration of substrate, while also improve the activity of glutamate decarboxylase, promote in brown rice embryo
Glutamic acid is effectively converted into γ-aminobutyric acid, so as to improve the content of γ-aminobutyric acid in sprouted unpolished rice.
It should be noted that it is enriched with a kind of aid from sprouting brown-rice germ that Chinese invention patent 200510010375.7 provides
The method of γ-aminobutyric acid is compared, 200510010375.7 detection be γ-aminobutyric acid in bud content, the present invention detection
Be γ-aminobutyric acid in whole sprouted unpolished rice content.
Embodiment 2
1) weigh the Ampelopsis grossedentata extrat of mass percent 47.50%, mass percent 16.25% Rosmarinus officinalis extract,
The vitamin B2 of mass percent 14.50%, the sodium glutamate of mass percent 10.05%, the dimension of mass percent 4.25%
Raw plain B6, the vitamin C of mass percent 4.75% and the folic acid of mass percent 2.70% are placed in blend tank, and stirring is equal
Even, the nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice is packed or produced after canned to compositing aluminium bag.
2) prepare 2 germination tanks, respectively take 5 kilograms by " brown rice is placed in germination tank made of the excellent 268 " paddy in day, and one is fallen
Enter 5 kilograms made of above-mentioned nutritional agents mass percent be 0.5% the nutritional agents aqueous solution, another pour into 5 kilograms it is pure
Water；
3) soaking temperature for setting 2 germination tanks is 27 DEG C, throughput is 1.2L/min, and immersion will be more after 20 hours
Remaining water discharge；
4) after water discharge unnecessary in 2 germination tanks, while 28 DEG C of germination temperature, throughput 1.5L/min are set, sent out
Bud stops germination after 24 hours；
5) germination tank is opened, sprouted unpolished rice is cleaned 2 times with running water, takes out sprouted unpolished rice after washing, then with 75 DEG C of temperature
The hot-water soak 10min of degree, water is drained；Brown rice after germination is less than content with microwave drying mode drying to moisture
Untill 13.5%.
6) " germination percentage, endogenous protease activities, the glutamate decarboxylase of the excellent 268 " brown rice in day in 2 germination tanks is detected
The content such as table 2 of activity and γ-aminobutyric acid：
" enzymatic activity changes the immersion of the different solutions of table 2 with alpha-aminobutyric acid content after the excellent 268 " brown rice germination in day
As can be seen from Table 2, " day excellent 268 " soaks germination in 1.5% vine tea extract solution, the germination percentage of brown rice, endogenous
When property proteinase activity, glutamate decarboxylase activity and alpha-aminobutyric acid content are soaked by pure water respectively 87.2%,
1.54U, 0.39U and 187.2mg/100g are respectively increased to 95.7%, 4.78U, 1.02U and 451.2mg/100g.It is main former
Because being that nutritional agents is soluble in water, the flow of water of solution is changed, reduces Premeabilisation of cells gesture, during brown rice absorbs water swollen, battalion
The uniformity that agent absorbs moisture as an infiltration matter regulation brown rice is supported, it is final to improve so as to promote the balanced germination of brown rice
The germination percentage of brown rice；In germination process, nutritional agents improves endogenous protease activities in brown rice, promotes protein in embryo
Hydrolysis, improve glutamate decarboxylase concentration of substrate, while also improve the activity of glutamate decarboxylase, promote in brown rice embryo
Glutamic acid is effectively converted into γ-aminobutyric acid, so as to improve the content of γ-aminobutyric acid in sprouted unpolished rice.
Embodiment 3
1) weigh the Ampelopsis grossedentata extrat of mass percent 47.50%, mass percent 16.75% Rosmarinus officinalis extract,
The vitamin B2 of mass percent 14.00%, the sodium glutamate of mass percent 10.25%, the dimension of mass percent 4.05%
Raw plain B6, the vitamin C of mass percent 4.45% and the folic acid of mass percent 3.00% are placed in blend tank, and stirring is equal
Even, the nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice is packed or produced after canned to compositing aluminium bag.
2) prepare 2 germination tanks, respectively take 5 kilograms made of " Boyou 998 " paddy brown rice be placed in germination tank, one is fallen
Enter 5 kilograms made of above-mentioned nutritional agents mass percent be 1.25% the nutritional agents aqueous solution, another pour into 5 kilograms it is pure
Water；
3) soaking temperature for setting 2 germination tanks is 35 DEG C, throughput is 2.0L/min, and immersion will be more after 25 hours
Remaining water discharge；
4) after water discharge unnecessary in 2 germination tanks, while 30 DEG C of germination temperature, throughput 2.0L/min are set, sent out
Bud stops germination after 25 hours；
5) germination tank is opened, sprouted unpolished rice is cleaned 3 times with running water, takes out sprouted unpolished rice after washing, then with 85 DEG C of temperature
The hot-water soak 8min of degree, water is drained；Brown rice after germination is less than content with microwave drying mode drying to moisture
Untill 13.0%.
6) germination percentage, endogenous protease activities, glutamate decarboxylase of " Boyou 998 " brown rice in 2 germination tanks are detected
The content such as table 3 of activity and γ-aminobutyric acid：
Enzymatic activity changes with alpha-aminobutyric acid content after the different solutions of table 3 immersion " Boyou 998 " brown rice germination
As can be seen from Table 3, " Boyou 998 " soaks germination in 2.0% vine tea extract solution, the germination percentage of brown rice, endogenous
When property proteinase activity, glutamate decarboxylase activity and alpha-aminobutyric acid content are soaked by pure water respectively 87.5%,
1.65U, 0.41U and 191.3mg/100g are respectively increased to 94.7%, 4.91U, 1.13U and 471.5mg/100g.It is main former
Because being that nutritional agents is soluble in water, the flow of water of solution is changed, reduces Premeabilisation of cells gesture, during brown rice absorbs water swollen, battalion
The uniformity that agent absorbs moisture as an infiltration matter regulation brown rice is supported, it is final to improve so as to promote the balanced germination of brown rice
The germination percentage of brown rice；In germination process, nutritional agents improves endogenous protease activities in brown rice, promotes protein in embryo
Hydrolysis, improve glutamate decarboxylase concentration of substrate, while also improve the activity of glutamate decarboxylase, promote in brown rice embryo
Glutamic acid is effectively converted into γ-aminobutyric acid, so as to improve the content of γ-aminobutyric acid in sprouted unpolished rice.
Embodiments of the present invention are simultaneously not restricted to the described embodiments, and other any spirit without departing from the present invention are real
Matter with made under principle change, modification, replacement, combine, simplification, should be equivalent substitute mode, be included in the present invention
Protection domain within.
Claims (10)
1. improve the nutritional agents of alpha-aminobutyric acid content in sprouted unpolished rice, it is characterised in that by mass percentage, its raw material
Form and be：
2. the nutritional agents according to claim 1 for improving alpha-aminobutyric acid content in sprouted unpolished rice, it is characterised in that press
Mass percent meter, its raw material composition are：
3. the nutritional agents according to claim 1 for improving alpha-aminobutyric acid content in sprouted unpolished rice, it is characterised in that institute
State in Ampelopsis grossedentata extrat, in terms of general flavone, weight/mass percentage composition >=40%, and wherein dihydromyricetin and myricetin monomer component
Mass ratio be 10~12：1.
4. the nutritional agents for improving alpha-aminobutyric acid content in sprouted unpolished rice according to claim 1 or 3, it is characterised in that
The Ampelopsis grossedentata extrat is to be prepared according to the following steps：Using the fresh cauline leaf of vine tea, done under the conditions of 35~45 DEG C of temperature
Vine tea dry feedstock is obtained after dry；Vine tea dry feedstock is obtained into vine tea extract solution using ethanol extraction method, then it is spray-dried
Method obtains Ampelopsis grossedentata extrat；
The mass ratio of vine tea dry feedstock and ethanol is 1 in the ethanol extraction method：10~12, the concentration of volume percent of ethanol
For 85~95%.
5. the nutritional agents according to claim 1 for improving alpha-aminobutyric acid content in sprouted unpolished rice, it is characterised in that institute
State in Rosmarinus officinalis extract, in terms of Rosmarinic acid, weight/mass percentage composition >=5%.
6. the nutritional agents of alpha-aminobutyric acid content in sprouted unpolished rice is improved according to claim 1 or 5, it is characterised in that
The Rosmarinus officinalis extract extract is to obtain by the following method：By fresh rosemary plant stem and leaf in 35~45 DEG C of bars of temperature
Rosemary plant dry feedstock is obtained after being dried under part；Rosemary plant dry feedstock is obtained into rosemary using ethanol extraction method
Plant extraction liquid, then spray-dried method obtain rosemary；Rosemary plant is dried in the ethanol extraction method
The mass ratio of raw material and ethanol is 1：10~12, the concentration of volume percent of ethanol is 85~95%.
7. the preparation method for improving the nutritional agents of alpha-aminobutyric acid content in sprouted unpolished rice described in claim 1, its feature exist
In, by proportioning weigh Ampelopsis grossedentata extrat, rosemary, vitamin B2, sodium glutamate, vitamin B6, vitamin C and
Folic acid raw material, stirs, and after compositing aluminium bag is packed or be canned, produces alpha-aminobutyric acid content in a kind of raising sprouted unpolished rice
Nutritional agents.
8. the application for improving the nutritional agents of alpha-aminobutyric acid content in sprouted unpolished rice described in claim 1, it is characterised in that
Including following operating procedure：
1) preparation of nutrition agent solution：The nutritional agents is dissolved into water, prepares the nutritional agents that mass concentration is 0.1%-5%
Solution；
2) immersion of brown rice：The nutrition agent solution of preparation is added in the brown rice for needing to germinate, the addition of nutrition agent solution is
All still expose liquid level 5-10cm after the full raw material of lid；Immersion more than 20 hours；
3) germination of brown rice：In germination process, stirred once per 8-10 hours, promote brown rice germination, it is a length of to the bud grown
0.5-1.2mm stops；
4) cleaning, enzymatic activity terminate：After germination terminates, germination raw material is cleaned with running water, germination raw material is taken out after washing, gone out
Enzyme, water is drained stand-by；
5) dry：By the brown rice raw material drying after germination.
9. the application according to claim 8 for improving the nutritional agents of alpha-aminobutyric acid content in sprouted unpolished rice, its feature exist
In the number that germination raw material is cleaned with running water is 2-3 times；Described enzyme deactivation is at the hot water with 75-85 DEG C of temperature
Manage more than 10min.
10. the application according to claim 8 for improving the nutritional agents of alpha-aminobutyric acid content in sprouted unpolished rice, its feature
It is, described drying is by the brown rice raw material microwave or sun dehydration after germination.Described drying is less than to moisture to be dry
Untill content 13%；Step 2) also includes discharging unnecessary nutrition agent solution after soaking more than 20 hours.
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