CN103238391B - Application of vine tea extract in improving germination rate of deteriorated seeds of rice - Google Patents
Application of vine tea extract in improving germination rate of deteriorated seeds of rice Download PDFInfo
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- CN103238391B CN103238391B CN201310199926.3A CN201310199926A CN103238391B CN 103238391 B CN103238391 B CN 103238391B CN 201310199926 A CN201310199926 A CN 201310199926A CN 103238391 B CN103238391 B CN 103238391B
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Abstract
The invention belongs to the technical field of rice seed priming research, and discloses an application of a vine tea extract in improving the germination rate of deteriorated seeds of hybrid rice. The vine tea extract is a vine tea extract product extracted and obtained by methods such as solvent extraction, ultrasonic extraction or chromatographic separation based on vine tea as a raw material. The product comprises a solid product, a liquid product and a paste product, preferably the solid product. The vine tea extract is a natural plant extract and is free from any toxicity and low in cost, and is free from environmental pollution, and used for initiating safety of seeds of hybrid rice. The vine tea extract has a remarkable effect on initiation of seeds of hybrid rice.
Description
Technical field
The invention belongs to rice paddy seed elicitation technique research field, particularly a kind of Ampelopsis grossedentata extrat is improving the application in paddy rice deterioration percentage of seedgermination.
Background technology
China is a big developing country having tens00000000 populations, and grain security is the Vital Strategic Problems of overall importance of relation economic development, social stability and national security all the time.Paddy rice is the staple food crop of China, accounts for national total output of grain over half, has extremely important status in grain security.The long-term sowing area of China paddy rice about 3,000 ten thousand hectares, year sowing quantity quite huge.The impact of the complexity of producing due to rice paddy seed and the difficult predictability of Seed Market, adds Global climate change, extreme climate and natural calamity frequently now, and normal needs carries out regulating between year to a large amount of rice paddy seed storage.But, cause because storage procedures is improper every year Rice Seed Vigor reduce, germination rate decline and scrap, cause huge economic loss, even badly influence the development of rice industry.According to incompletely statistics, the loss that China causes because of Deterioration, storage pest in rice storage process and impact of going mouldy every year accounts for 3% of gross storage capacity, reaches tens billion of jin, and annual loss is not less than 20,000,000,000 yuan.Due to conventional Rice and hybrid rice seed store the germination rate that causes decline bring directly and indirect economic loss huge especially.
At present, many in the research improving rice paddy seed storage tolerance technology or seed vitality maintenance technical elements, but after seed deterioration, the research how saved seed embryo vigor is weaker.Therefore, when occurring along with accident (as meeting time or time of suffering a calamity etc. of the poor harvest of brand-new seed), by rice paddy seed elicitation technique, rescue improves the embryo vigor of deterioration seed or recovers seed vitality and deterioration seed application is seemed particularly important in Production of Large Fields.
Seed priming (Seed Priming) is a kind of new technology improving seed vitality, and essence belongs to seed embryo vigor rescue category.Seed priming is also called osmotic adjustment (Osmo-conditioning), proposed in 1973 by Heydeker the earliest, it slowly absorbs water by controlling seed, it is made to rest on the second stage of imbibition, seed is allowed to carry out presprouting, promote cell membrane, organelle, the reparation of DNA and the activation of enzyme, make it to be in the SBR of germination but prevent stretching out of radicle.Seed priming techniques proposes based on the biological mechanism of seed germination, and object promotes seed germination, and improve the coefficient of stabilization of sprout time and sprout neat rate, reduces the standard deviation of sprout time, improve resistance and the quality of seedling, improve nutrition condition.Large quantity research shows, through the seed caused, vigor obviously strengthens, and germination rate, planting percent and resistance significantly improve, and emerge fast and together, output increases, quality also improves.
Along with the development of science and technology, seed priming research deepens continuously, the method caused also constantly is expanded, mainly contain the various ways such as liquid initiation, solid matrix initiation, cylinder initiation, biological initiation and film initiation at present, achieve the goal mainly through osmotic adjustment, temperature adjustment, gas regulation and hormone control etc.At present about the research of seed priming concentrates on horticultural crop or the seed such as tobacco, the herbage fields such as fruit tree, vegetables, flowers mostly, the research of its triggering mechanism receives more concerns, and application approach and prospect are also in continuous expansion.By contrast, in recent years to rice paddy seed particularly hybrid rice seeds cause research fewer.Relevant rice paddy seed initiating method mainly contains following:
One is take water, PEG, PVA, KNO
3, KH
2pO
4, CaCl
2, gibberellin, salicylic acid etc. carry out osmotic adjustment, wherein causes effect with PEG again better.PEG(chemistry polyethylene glycol by name) be a kind of high molecular polymer, chemical stability is high, not through cell wall, thus the biochemical reaction of cell is not affected, PEG solution carrys out degree and the state of regulating cell water suction by colloid osmotic potential, the water suction of seed can be made to tend towards stability and synchronization, final raising germination rate and neat rate, effectively more than adopting single water soaking to cause.
Two is utilize antibiotic or rare-earth elements La or Ce Rice seeds treated, and after process, seed vitality obviously increases.
Three is adopt solid matrix to cause, and as vermiculite, shale, fine sand etc., controls Seed imbibes amount thus reach initiation object by solid matrix.
Although various initator has respective feature, shortcoming also clearly: PEG has the character of colloid, and its solution can obtain the lower flow of water, and it is to seed nonhazardous effect, but viscosity is comparatively large, and in solution, oxygen solubility is low, and large usage quantity, cost is high, the surface of the seed content of molds rising etc.The PVA mechanism of action and PEG similar, there is consumption few, the advantage that cost is low, but cause effect undesirable, do not reach production requirement.
Inorganic ion is general, and all consumption is few, advantage of lower cost, but the ion of mineral salt can infiltrate seed and accumulate in seed in processing procedure, thus reduces seed osmotic potential, causes neutron absorption more juicy and reduce germination rate.Water initiation may cause seed moisture absorption inequality to cause, and Seed imbibition injures, the surface of the seed content of molds rises.
Suitable hormone (as gibberellin etc.) the process usual life-span to fresh seeds, vitality or germinating capacity have facilitation, but with HORMONE TREATMENT deterioration seed, except indivedual hormone or the reduction of concentration to seed vitality not obvious except, general trend for significantly to suppress seed germination by HORMONE TREATMENT, so be not a kind of recovery and the good method improving deterioration seed vitality with HORMONE TREATMENT deterioration seed.
Rice paddy seed is after antibiotic, rare-earth elements La or Ce process, although seed vitality obviously increases, but the problems such as the side effect of the toxicity of medicament and environmental pollution should not be underestimated, particularly rare earth easily accumulates in agricultural land soil, can have influence on the sustainable use of field ecosystem to the crop negative effect that even field ecosystem produces.
There is seed and be separated more difficult problem with matrix in solid matrix initiation.
The physical means such as other utilization high-voltage electrostatic field, simulated microgravity process carry out the method for deterioration seed vitality recovery, Special Equipment, cost need be had high, also likely occur the possibility of gene mutation.
Vine tea is a kind of important class tea plant of China, Plant Taxonomy belongs to porcelain ampelopsis kind in Vitaceae Ampelopsis, various places are commonly called as a lot, and if Guangdong, Guangxi is also known as " white tea ", " white tea cake ", " rabdosia lophanthide tea ", Zhangjiajie, Hunan is also called " the mould tea of Mao Yan ".Monograph " vine tea " has done detailed discussion (work such as Zhang Yousheng, Guangdong Science Press publishes in August, 2003) to this.Publication number CN1288747A, CN1288892A etc. have disclosed this extract to be had treatment hepatitis, liver protecting, anti-inflammation, analgesia, reducing blood lipid, eliminates the phlegm and strengthen the effect of immune system immunity and the method by the method extraction and isolation dibydro myricetrins such as solvent extraction, ultrasonic extraction, chromatographic isolation and composition thereof.Patent No. ZL20041002778204 has disclosed the method that this extract is undertaken by microwave method extracting.Patent No. ZL2004100516327 has disclosed the purposes that this extract can be used as natural fruit and vegetables and fruit and vegetable product color stabilizer.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art part, the object of the present invention is to provide a kind of Ampelopsis grossedentata extrat improving the application in paddy rice deterioration percentage of seedgermination, being applied to the raising of paddy rice deterioration percentage of seedgermination by Ampelopsis grossedentata extrat as initator.
Object of the present invention is realized by following technical proposals: a kind of Ampelopsis grossedentata extrat is improving the application in paddy rice deterioration percentage of seedgermination.
Described Ampelopsis grossedentata extrat refers to vine tea to be raw material, extracts the Ampelopsis grossedentata extrat product obtained, comprise solid product, fluid product and creamy product, preferred solid product by methods such as solvent extraction, ultrasonic extraction or chromatographic isolation.
Described application comprises following operating procedure: use first 0.5 ~ 1 month at paddy rice deterioration seed, with water, the solution that mass percent is 0.1 ~ 0.5% is made in Ampelopsis grossedentata extrat dissolving, again paddy rice deterioration seed is soaked wherein, soaking position is that solution exceeds seed top 1 ~ 2cm, soak after 12 ~ 24 hours, seed is taken out from solution and drains the water, then preserved to seed safety storage water content by seed Hui Gan, described Hui Gan dries or is placed in less than 35 DEG C oven for drying after the seed after draining the water being placed in shady and cool dry place's natural seasoning.Or cause before paddy rice deterioration seed presoaking and germinating, with water, the solution that mass percent is 0.1 ~ 0.5% is made in Ampelopsis grossedentata extrat dissolving, again paddy rice deterioration seed is soaked wherein, soaking position is that solution exceeds seed top 1 ~ 2cm, soak after 12 ~ 24 hours, seed baking oven below 35 DEG C is returned dry 24 ~ 48 hours, enters seed presoaking and germinating process immediately, improve deterioration percentage of seedgermination with this.
Described rice paddy seed refers to the various rice varieties (being) used in China through national correlation department approval, preferred Hybrid Rice Combinations (strain).
Principle of the present invention is: Ampelopsis grossedentata extrat is soluble in water can reduce Premeabilisation of cells gesture by the flow of water changing solution.During seed priming, Ampelopsis grossedentata extrat is as the speed of an infiltration matter not only adjustable neutron absorption moisture, also produces actively impact to seed metabolic activity simultaneously.In elicitation procedure, the physics that initiation can produce biofilm system is repaired and Physiology and biochemistry repairing.Superoxide dismutase (SOD) after seed priming, the antioxidation systems such as peroxidase (POD) and catalase (CAT) improve, the ability of Scavenger of ROS strengthens, lipid peroxidation alleviates; The enzymic activity such as dehydrase and acid phosphatase also improves; Obtain higher amylase activity simultaneously, accelerate the capacity of decomposition of carbohydrate; Soluble protein content significantly improves, and seed leachate electrical conductivity, MDA and soluble sugar content significantly reduce.In Osmoregulation Process, Interior Seed there occurs the metabolic process that some promotes to sprout, thus decreases the time in Seed Germination needed for these metabolism.
Hinge structure of the present invention, has following advantage and beneficial effect:
(1) Ampelopsis grossedentata extrat system natural plant extracts, without any toxicity, price is low, as the initiation safety of rice paddy seed, non-environmental-pollution.
(2) Ampelopsis grossedentata extrat is to the initiation successful of rice paddy seed.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1:
Adopt the fresh cauline leaf of vine tea, under temperature 35 ~ 45 DEG C of conditions, obtain vine tea dried feed after drying; Adopt ethanol extraction method to obtain vine tea extract vine tea dried feed, more spray-dried method obtain Ampelopsis grossedentata extrat; In described ethanol extraction method, the mass ratio of vine tea dried feed and ethanol is 1:10 ~ 12, and the concentration of volume percent of ethanol is 85 ~ 95%.By the Ampelopsis grossedentata extrat compositing aluminium bag of above-mentioned gained packaging or canned after namely obtain paddy rice deterioration seed initiator.
Embodiment 2
Get the common vine tea 100.0g that market is bought, add 500g water, be placed in household microwave oven, extract 2 minutes, filtered through gauze, cooling filtrate 10 minutes, namely Ampelopsis grossedentata extrat is deposited in container bottom.Dry sediment, is weighed as 16.5g, is Ampelopsis grossedentata extrat.By the Ampelopsis grossedentata extrat compositing aluminium bag of above-mentioned gained packaging or canned after namely obtain paddy rice deterioration seed initiator.
Embodiment 3: paddy rice deterioration seed causes the germination after process through Ampelopsis grossedentata extrat
" causing process " concrete grammar in this experimentation is as follows: CK is experiment contrast, and the rice paddy seed namely got without any process is directly undertaken by " Germination Percentage of Rice assay method " (GB/T3543); " clear water 12h " and " clear water 24h " will measure the germination rate of rice paddy seed after being treated to and paddy rice deterioration seed being soaked 12h and 24h respectively in clear water by GB/T3543 method after seed Hui Gan; " 0.10% Ampelopsis grossedentata extrat ", " 0.25% Ampelopsis grossedentata extrat " and " 0.50% Ampelopsis grossedentata extrat " are treated to gets 0.10 kilogram, 0.25 kilogram and 0.50 kilogram of embodiment 1 gained Ampelopsis grossedentata extrat heating for dissolving in clear water respectively, then add water and solution is settled to 100 kilograms namely obtains " 0.10% Ampelopsis grossedentata extrat ", " 0.25% Ampelopsis grossedentata extrat " and " 0.50% Ampelopsis grossedentata extrat " solution, then paddy rice deterioration seed is put into after wherein soaking 12h and surveys germination rate by after seed Hui Gan.
Table 1 and table 2 are the data variation (Hybrid Rice Combinations material is provided by Golden Rice Seeds Co., Ltd. of Guangdong) of two different Hybrid Rice Combinations germination rate after causing process below, as can be seen from Table 1 and Table 2, after 0.10% Ampelopsis grossedentata extrat, 0.25% Ampelopsis grossedentata extrat and 0.50% Ampelopsis grossedentata extrat initiation process, the germination rate of rice paddy seed significantly improves.
The change of table 1 " Qiu You 998 " Crosses of Rice germination rate after causing
The change of table 2 " sky excellent 368 " Crosses of Rice germination rate after causing
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (4)
1. an Ampelopsis grossedentata extrat is improving the application in paddy rice deterioration percentage of seedgermination, it is characterized in that: described Ampelopsis grossedentata extrat prepares in accordance with the following methods: the fresh cauline leaf adopting vine tea, obtains vine tea dried feed after drying under temperature 35 ~ 45 DEG C of conditions; Adopt ethanol extraction method to obtain vine tea extract vine tea dried feed, more spray-dried method obtain Ampelopsis grossedentata extrat; In described ethanol extraction method, the mass ratio of vine tea dried feed and ethanol is 1:10 ~ 12, and the concentration of volume percent of ethanol is 85 ~ 95%; Described Ampelopsis grossedentata extrat dissolve make mass percent be 0.25% or 0.5% solution be applied to and improve paddy rice deterioration percentage of seedgermination.
2. application according to claim 1, it is characterized in that: described application comprises following operating procedure: use first 0.5 ~ 1 month at paddy rice deterioration seed, with water, the solution that mass percent is 0.25% or 0.5% is made in Ampelopsis grossedentata extrat dissolving, again paddy rice deterioration seed is soaked wherein, soaking position is that solution exceeds seed top 1 ~ 2cm, soak after 12 ~ 24 hours, seed is taken out from solution and drains the water, then seed Hui Gan is preserved to seed safety storage water content.
3. application according to claim 2, is characterized in that: described Hui Gan dries or be placed in less than 35 DEG C oven for drying after the seed after draining the water being placed in shady and cool dry place's natural seasoning.
4. application according to claim 1, it is characterized in that: described application comprises following operating procedure: cause before paddy rice deterioration seed presoaking and germinating, with water, the solution that mass percent is 0.25% or 0.5% is made in Ampelopsis grossedentata extrat dissolving, again paddy rice deterioration seed is soaked wherein, soaking position is that solution exceeds seed top 1 ~ 2cm, soak after 12 ~ 24 hours, seed baking oven below 35 DEG C is returned dry 24 ~ 48 hours, enters seed presoaking and germinating process immediately.
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| US9756775B2 (en) * | 2014-07-07 | 2017-09-12 | Cherished Ones Llc | Memorial flower planting product made with or without human or pet cremated remains |
| CN107517955B (en) * | 2017-08-18 | 2021-01-26 | 广东省农业科学院农业生物基因研究中心 | Composite rice seed life prolonging agent and preparation method and application thereof |
| CN107347299B (en) * | 2017-08-21 | 2021-04-06 | 劲家庄(惠州)健康食品有限公司 | Method for improving content of gamma-aminobutyric acid in germinated coarse grain raw material by using vine tea extract |
| CN114557353B (en) * | 2022-03-09 | 2023-11-07 | 广东省农业科学院农业生物基因研究中心 | Composition for regulating harvesting period of rice seeds and preparation method and application thereof |
| CN114557352B (en) * | 2022-03-09 | 2024-02-02 | 广东省农业科学院农业生物基因研究中心 | Composition for promoting early harvesting of rice seeds as well as preparation method and application thereof |
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| JP2005139070A (en) * | 2003-11-04 | 2005-06-02 | Kanebo Cosmetics Inc | Skin cosmetic |
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| CN102018083A (en) * | 2010-11-16 | 2011-04-20 | 张春枝 | Production method of fermented vine tea, vine tea extract and vine tea drink |
| CN102058532A (en) * | 2010-12-30 | 2011-05-18 | 张家界茅岩莓有限公司 | Compound dihydromyricetin oral aerosol and preparation method thereof |
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