CN107496905A - A kind of Chinese medicine composition for improving dam fecundity and preparation method thereof - Google Patents
A kind of Chinese medicine composition for improving dam fecundity and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to technical field of veterinary, and in particular to a kind of Chinese medicine composition for improving dam fecundity and preparation method thereof.The Chinese medicine composition is mainly prepared from the following raw materials in parts by weight：5~10 parts of cecropin antimicrobial peptides, 10~20 parts of barrenwort, 15~20 parts of motherwort, 8~15 parts of safflower, 8~15 parts of the red sage root, 15~20 parts of the red meals of sheep, 5~15 parts of honeysuckle, 2~5 parts of bacillus subtilis.Cecropin antimicrobial peptides have the anti-microbial property of wide spectrum, are a kind of natural antibiotic, are acted on bacillus subtilis, repair endometrial impairment, balance endometrium microorganism species；Cecropin antimicrobial peptides, bacillus subtilis and traditional Chinese medicine ingredients Synergistic, body is promoted to absorb traditional Chinese medicine ingredients, traditional Chinese medicine ingredients are promoted to play efficacy of drugs, therapeutic effect of the Chinese medicine composition to infertilities such as dam endometritis, inactive ovaries and persistentcorpus luteum infertility, retentions of afterbirth is lifted, lifts the conception rate of dam.
Description
Technical field
The present invention relates to technical field of veterinary, and in particular to it is a kind of improve dam fecundity Chinese medicine composition and
Its preparation method.
Background technology
As the improvement of people's living standards, people are continuously increased to the demand of the meat products such as pork, beef, mutton,
The supply of meat products must be increased by increasing the quantity of dam, speculated according to the dam raised at present, present livestock
It is in large supply in demand, it is but true really not so.In recent years, often there is the present situation that supply falls short of demand in meat products, pork,
The meat products such as beef, mutton hold at high price.Here in addition to epidemic disease reason and nutrition reason, the production with dam itself
Ability declines closely related.The production capacity of dam is low, is mainly shown as that Oestrus rate is low, breeding rate is low, litter size is few, causes
Breeding efficiency declines.The survey showed that, due to infertility proportion highest caused by endometritis, next to that ovary is quiet
Only and retained corpus luteum.
Cause to breed During Estrous Cycle exception during ovary inactive infertility, reproductive ability is low, causes cow infertile
One of the main reasons, static including cow ovaries, retained corpus luteum, ovary are static, ovarian cyst, ovarian follicle are alternately developed, row
A variety of diseases such as ovum delay.Because such disease incidence is high, influence cattle-raising and receive than more serious all to close very much both at home and abroad
Note.N.Hollis has found that only ovarian cyst ox just has 210 within 150 days postpartum to 3172 cow head incidence periodic statisticals
Head, the incidence of disease 6.26%；Ahmadljaz etc. reports that milk cow ovarian cyst probability is 6-19%；Northeast Agricultural University is to 6158
Cow head is investigated, and suffers from disease of ovary milk cow 325, the incidence of disease 5.29%, wherein Monitoring Ovarian Function be not complete 124, accounts for ovary disease
The 38.15% of disease, retained corpus luteum 45,13.85% being accounted for, ovarian cyst 123, accounts for 37.85%, ovarian follicle alternately develops 25,
7.69% is accounted for, delayed ovulation 8, accounts for 2.46%；Lanzhou Livestock and Animal Drug Inst., Chinese Academy of Agricultural Science is to Heilungkiang, day
The provinces such as Tianjin, Jiangsu, Hunan, Gansu, Sichuan, Hebei, 21596 mature cows of 83 cattle farms in city are just investigated,
Not calver 1830 caused by disease of ovary, account for 8.45%, wherein retained corpus luteum 555, account for the 30.32% of disease of ovary,
Static 443 of ovary, 24.20% is accounted for, ovarian cyst 379,21.69% is accounted for, ovarian atrophy 184, accounts for 10.05%, ovulation
Delay 64, accounts for 3.50%, ovary, salpingitis 70, accounts for 3.82%, and ovary and fallopian tubal Other diseases are (ovarian neoplasm, defeated
Oviduct hardens, is adhered) 135, account for 7.38%.From secondary, disease of ovary infertility is milk cow common disease and frequently-occurring disease.
The domestic research to dam infertility at present has been achieved for good achievement, but the medicine studied is most
Number is chemicals, and price is of a relatively high, and the western animal doctor of the treatment for endometritis mainly applies antibiotic and anti-corrosion
Sterilization class medicine just carries out uterine irrigation and perfusion, although this method is effective, needs Reusability, and frequently use antibiosis
Element can cause some bacterial resistance occurred so that therapeutic effect constantly increases with dosage worse and worse, long-term use of antibiosis
Element can increase the residual quantity in livestock products, bring food-safety problem.Treatment west for inactive ovaries and persistentcorpus luteum in addition
Animal doctor mainly uses hormonotherapy, but is difficult to move Expected Results, also easily causes the hormone residues in livestock products, endangers the mankind and be good for
Health.
Clinically following side effect be present using hormone medicine treatment disease of ovary infertility at present：Clinically more
Carry out cow ovary active situation according to examination per rectum, because the clinical experience of technical operation person is different, easily cause subjective erroneous judgement,
Select medicine hormone improper.So easily cause the regulation of body reproductive hormone disorderly, make dam be not easy to become pregnant；Raiser is
Increase economic efficiency, it is a large amount of, hormone therapy is applied multiple times, easily dam body is damaged, and largely, be applied multiple times it is sharp
Extract for treating, hormone residues in meat, milk are easily caused, threaten human health.
In order to overcome the side effect of above-mentioned hormone medicine, engender the medicine of some Chinese medicine preparation classes to dam ovum
The disease infertility of nest is treated, but the treatment effective traditional Chinese medicine ingredients of dam disease of ovary infertility are single at present, in
Two, state's veterinary drug shop (version in 2000) kind recorded only has 2 kinds, for promote the sexual maturity dissipate and progestogenic perfusion liquid, be ancient books classical Chinese medicine
Compound, there is scholar to carry out pharmacological research at present, although curative for effect, production technology is more extensive, quality standard need after
It is continuous to improve, to carry out large-scale production；Existing Chinese medicine class treats dam disease of ovary infertility Chinese medicine wholistic therapy effect
Bad, low to dam disease of ovary infertility cure rate, conception rate is low.
The content of the invention
The defects of in order to overcome prior art, it is an object of the invention to provide a kind of Chinese medicine group for improving dam fecundity
Compound, dam disease of ovary infertility can be significantly treated, promote dam heat, improve the fecundity of dam.
Meanwhile the present invention also resides in the preparation method for providing a kind of Chinese medicine composition for improving dam fecundity.
In order to realize the above object the technical solution adopted in the present invention is：
A kind of Chinese medicine composition for improving dam fecundity, is mainly prepared by the raw material of following parts by weight：My god
5~10 parts of sbombycin antibacterial peptide, 10~20 parts of barrenwort, 15~20 parts of motherwort, 8~15 parts of safflower, 8~15 parts of the red sage root, sheep are red
15~20 parts of meals, 5~15 parts of honeysuckle, 2~5 parts of bacillus subtilis.
Optionally, the formulation of the Chinese medicine composition is perfusion liquid；It further comprises in the perfusion liquid preparation process following
The raw material of parts by weight：10~20 parts of glycerine, 1~5 part of polyvinylpyrrolidone, 0.15~0.5 part of preservative, antioxidant
0.01~0.1 part.
The preparation method of the Chinese medicine composition of above-mentioned raising dam fecundity, including following operating procedure：
1) effective component of chinese medicine extract is extracted：The red meals of barrenwort, motherwort, safflower, the red sage root, sheep are weighed in parts by weight
Crushed after being mixed with honeysuckle, with supercritical CO2Fluid is extractant, is entered using the mixed solvent of water, ethanol and acetone as entrainer
Row extraction, carries out decompression separation after extraction, discharge, obtain effective component of chinese medicine extract；
2) perfusion liquid is prepared：The effective component of chinese medicine for taking step 1) to prepare, add cecropin antimicrobial peptides, bacillus subtilis
After bacterium, glycerine, polyvinylpyrrolidone, preservative and antioxidant are well mixed, distilled water constant volume is added, obtains perfusion liquid.
Optionally, the specific method of extraction effective component of chinese medicine is in step 1)：
A：The red meals of barrenwort, motherwort, safflower, the red sage root, sheep and honeysuckle are weighed in parts by weight, and extraction is added after co-grinding
Take in device, supercritical CO is passed through in extractor2Fluid and entrainer, in 30~40MPa pressure, 40~75 DEG C of temperature conditionss
Under, extract 3~5h；
B：Successively by primary separator, second-stage separator after the completion of step A extractions, by effective component of chinese medicine and overcritical
CO2Fluid is separated, wherein supercritical CO2Fluid returns to be recycled in extractor, and primary separator discharging is a small amount of sticky
Property big traditional Chinese medicinal ointment, detect no effective component of chinese medicine, second-stage separator discharging is the effective component of chinese medicine extract of brown, separation
Post discharging is the entrainer liquid of clarification；Wherein supercritical CO2Fluid flow through primary separator carry out first separation pressure be
30MPa, temperature are 50~75 DEG C；The pressure for flowing through second-stage separator progress secondary separation is 30MPa, temperature is 35 DEG C.
Optionally, the entrainer is water, ethanol, acetone with volume ratio 1：4~7:2~5 mixed solution.
Optionally, the quality dosage of the entrainer is barrenwort, motherwort, safflower, the red sage root, the red meals of sheep and honeysuckle quality
The 5~20% of total amount.
Optionally, supercritical CO2The flow of fluid is 15~30L/h.
The present invention improves the Chinese medicine composition of dam fecundity, and its formulation is perfusion liquid, available for treatment dam uterus
The infertilities such as intimitis, inactive ovaries and persistentcorpus luteum infertility, retention of afterbirth, the fecundity of dam is lifted, it is specific to use
Method is：By apparatus and dam external genital routine disinfection, hold is sent vas deferens with the method similar to sow semen deposition or uterus
Enter uterus, inject perfusion liquid 100ml of the present invention every time, 1 time a day, be used in conjunction 3 days, 3 times are 1 course for the treatment of；
Beneficial effect of the present invention：
1) present invention improve dam fecundity Chinese medicine composition, with cecropin antimicrobial peptides, bacillus subtilis, with it is excessive
The six kinds of traditional Chinese medicine ingredients collaborations of the red meals of the sheep leaves of pulse plants, motherwort, safflower, the red sage root, sheep and honeysuckle, which use, is used as key component, wherein cecropin
Antibacterial peptide has the anti-microbial property of wide spectrum, to 12 kinds of germ tools such as pathogenic bacteria salmonella, Escherichia coli, staphylococcus aureus
There is very strong bactericidal action, be a kind of natural antibiotic, it is safe and efficient, do not develop immunity to drugs, noresidue side effect；And day
Sbombycin antibacterial peptide acts on bacillus subtilis, repairs endometrial impairment, balances endometrium microorganism species；Cecropin resists
Bacterium peptide, bacillus subtilis and traditional Chinese medicine ingredients Synergistic, promote body to absorb traditional Chinese medicine ingredients, promote traditional Chinese medicine ingredients to play medicine
Effect, lifting Chinese medicine composition are infertile to dam endometritis, inactive ovaries and persistentcorpus luteum infertility, retention of afterbirth etc.
The therapeutic effect of disease, lift the conception rate of dam so that conception rate is promoted to more than 80%, even up to 100%.Meanwhile enter
One step by the way of perfusion liquid uterine perfusion, medicine is played maximum drug effect；
2) in most of traditional Chinese medicine extractions, boiling decoction is typically taken, makes the loss of effective components of Chinese medicine more, especially
Water insoluble active ingredient, cause to waste.The present invention takes CO to overcome deficiency of the prior art2Supercritical fluid extraction skill
Art, supercritical extraction technique resulting solution remains less, is less toxic, pollution-free, in being avoided using CO2 supercritical liquid extraction techniques
The drawbacks of medicine is added in the conventional fabrication process that decocting is endured, the oxidation and loss of heat-sensitive substance are effectively prevented, so as to improve curative effect；
SFE can more effectively extract the volatile component in complicated Chinese medicine, the complete bioactivity for retaining medicine, and can be height boiling
Point, low volatility, the material that is easily pyrolyzed once extract in its boiling temperature, and SFE makes extraction and separation integrate, when full
When CO-SF containing dissolved matter flows through separator, CO2 and extract is caused to rapidly become two-phase (gas-liquid separation) because pressure declines
And separate rapidly, not only extraction efficiency is high but also energy consumption is less, economical pollution-free, and extraction fluid can be recycled, and really realizes
Production process greenization；
3) further, the present invention limits the pressure, temperature, time of extraction during traditional Chinese medicine extyaction, limits
Surely the second-order separation is used, and limits temperature, the pressure separated every time, limits supercritical fluid CO2Flow, it is ensured that maximum limit
The active ingredient extracted in Chinese medicine, lift the purity of effective component of chinese medicine, the final curative effect of medication for improving Chinese medicine composition.
Embodiment
Technical scheme is described in detail below by specific embodiment.
Embodiment 1
A kind of Chinese medicine composition for improving dam fecundity, its formulation is perfusion liquid, by the raw material of following parts by weight
It is prepared：8 parts of cecropin antimicrobial peptides, 15 parts of barrenwort, 18 parts of motherwort, 12 parts of safflower, 12 parts of the red sage root, 18 parts of the red meals of sheep,
10 parts of honeysuckle, 4 parts of bacillus subtilis, 15 parts of glycerine, 4 parts of polyvinylpyrrolidone, 0.25 part of preservative, antioxidant 0.07
Part.
The preparation method of the perfusion liquid of above-mentioned raising dam fecundity, including following operating procedure：
1) effective component of chinese medicine is extracted：
A：The red meals of barrenwort, motherwort, safflower, the red sage root, sheep and honeysuckle are weighed in parts by weight, and extraction is added after co-grinding
Take in device, supercritical CO is passed through according to 20L/h flows in extractor2Fluid and entrainer, in 35MPa pressure, 55 DEG C of temperature
Under the conditions of, extract 4h；Wherein entrainer is water, ethanol, acetone with volume ratio 1：5：3 mixed solution, dosage are barrenwort, benefit
The 12% of the red meals of Brittle Falsepimpernel Herb, safflower, the red sage root, sheep and honeysuckle quality total amount；
B：Successively by primary separator, second-stage separator after the completion of step A extractions, by effective component of chinese medicine and overcritical
CO2Fluid is separated, wherein supercritical CO2Fluid returns to be recycled in extractor, and primary separator discharging is a small amount of sticky
Property big traditional Chinese medicinal ointment, detect no effective component of chinese medicine, second-stage separator discharging is the effective component of chinese medicine extract of brown, separation
Post discharging is the entrainer liquid of clarification；Wherein supercritical CO2Fluid flow through primary separator carry out first separation pressure be
30MPa, temperature are 62 DEG C；The pressure for flowing through second-stage separator progress secondary separation is 30MPa, temperature is 35 DEG C.
2) perfusion liquid is prepared：The effective component of chinese medicine for taking step 1) to prepare, add cecropin antimicrobial peptides, bacillus subtilis
Bacterium, glycerine, PVP, preservative and antioxidant are well mixed, and are added distilled water constant volume, are obtained perfusion liquid.
Embodiment 2
A kind of Chinese medicine composition for improving dam fecundity, its formulation is perfusion liquid, by the raw material of following parts by weight
It is prepared：5 parts of cecropin antimicrobial peptides, 10 parts of barrenwort, 15 parts of motherwort, 8 parts of safflower, 8 parts of the red sage root, 15 parts of the red meals of sheep, honeysuckle 5
Part, 2 parts of bacillus subtilis, 10 parts of glycerine, 1 part of polyvinylpyrrolidone, 0.15 part of preservative, antioxidant 0.0.1 parts.
The preparation method of the perfusion liquid of above-mentioned raising dam fecundity, including following operating procedure：
1) effective component of chinese medicine is extracted：
A：The red meals of barrenwort, motherwort, safflower, the red sage root, sheep and honeysuckle are weighed in parts by weight, and extraction is added after co-grinding
Take in device, supercritical CO is passed through according to 15L/h flows in extractor2Fluid and entrainer, in 30MPa pressure, 40 DEG C of temperature
Under the conditions of, extract 5h；Wherein entrainer is water, ethanol, acetone with volume ratio 1：4：2 mixed solution, dosage are barrenwort, benefit
The 5% of the red meals of Brittle Falsepimpernel Herb, safflower, the red sage root, sheep and honeysuckle quality total amount；
B：Successively by primary separator, second-stage separator after the completion of step A extractions, by effective component of chinese medicine and overcritical
CO2Fluid is separated, wherein supercritical CO2Fluid returns to be recycled in extractor, and primary separator discharging is a small amount of sticky
Property big traditional Chinese medicinal ointment, detect no effective component of chinese medicine, second-stage separator discharging is the effective component of chinese medicine extract of brown, separation
Post discharging is the entrainer liquid of clarification；Wherein supercritical CO2Fluid flow through primary separator carry out first separation pressure be
30MPa, temperature are 50 DEG C；The pressure for flowing through second-stage separator progress secondary separation is 30MPa, temperature is 35 DEG C.
2) perfusion liquid is prepared：The effective component of chinese medicine for taking step 1) to prepare, add cecropin antimicrobial peptides, bacillus subtilis
Bacterium, glycerine, PVP, preservative and antioxidant are well mixed, and are added distilled water constant volume, are obtained perfusion liquid.
Embodiment 3
A kind of Chinese medicine composition for improving dam fecundity, its formulation is perfusion liquid, by the raw material of following parts by weight
It is prepared：10 parts of cecropin antimicrobial peptides, 20 parts of barrenwort, 20 parts of motherwort, 15 parts of safflower, 15 parts of the red sage root, 20 parts of the red meals of sheep,
15 parts of honeysuckle, 5 parts of bacillus subtilis, 20 parts of glycerine, 5 parts of polyvinylpyrrolidone, 0.5 part of preservative, 0.1 part of antioxidant.
The preparation method of the perfusion liquid of above-mentioned raising dam fecundity, including following operating procedure：
1) effective component of chinese medicine is extracted：
A：The red meals of barrenwort, motherwort, safflower, the red sage root, sheep and honeysuckle are weighed in parts by weight, and extraction is added after co-grinding
Take in device, supercritical CO is passed through according to 30L/h flows in extractor2Fluid and entrainer, in 40MPa pressure, 75 DEG C of temperature
Under the conditions of, extract 3h；Wherein entrainer is water, ethanol, acetone with volume ratio 1：7：5 mixed solution, dosage are barrenwort, benefit
The 20% of the red meals of Brittle Falsepimpernel Herb, safflower, the red sage root, sheep and honeysuckle quality total amount；
B：Successively by primary separator, second-stage separator after the completion of step A extractions, by effective component of chinese medicine and overcritical
CO2Fluid is separated, wherein supercritical CO2Fluid returns to be recycled in extractor, and primary separator discharging is a small amount of sticky
Property big traditional Chinese medicinal ointment, detect no effective component of chinese medicine, second-stage separator discharging is the effective component of chinese medicine extract of brown, separation
Post discharging is the entrainer liquid of clarification；Wherein supercritical CO2Fluid flow through primary separator carry out first separation pressure be
30MPa, temperature are 75 DEG C；The pressure for flowing through second-stage separator progress secondary separation is 30MPa, temperature is 35 DEG C.
2) perfusion liquid is prepared：The effective component of chinese medicine for taking step 1) to prepare, add cecropin antimicrobial peptides, bacillus subtilis
Bacterium, glycerine, PVP, preservative and antioxidant are well mixed, and are added distilled water constant volume, are obtained perfusion liquid.
Comparative example 1
This comparative example replaces with mammal antibacterial as different from Example 1, by the cecropin antimicrobial peptides in embodiment 1
Peptide, other are the same as embodiment 1.
Comparative example 2
This comparative example replaces with fish antibacterial peptide as different from Example 1, by the cecropin antimicrobial peptides in embodiment 1,
Other are the same as embodiment 1.
Comparative example 3
This comparative example as different from Example 1, saves the cecropin antimicrobial peptides in embodiment 1 and bacillus subtilis,
And the dosage for adjusting effective component of chinese medicine is equal to cecropin antimicrobial peptides+bacillus subtilis+effective component of chinese medicine in embodiment 1
Dosage sum.
Comparative example 4
This comparative example as different from Example 1, saves bacillus subtilis and traditional Chinese medicine ingredients, and adjusts cecropin antibacterial
The dosage of peptide is equal to the dosage sum of cecropin antimicrobial peptides+bacillus subtilis+effective component of chinese medicine in embodiment 1.
Comparative example 5
This comparative example as different from Example 1, saves cecropin antimicrobial peptides and traditional Chinese medicine ingredients, and adjust bacillus subtilis
The dosage of bacterium is equal to the dosage sum of cecropin antimicrobial peptides+bacillus subtilis+effective component of chinese medicine in embodiment 1.
Test example 1
1st, experimental site
Auspicious hereditary Co., Ltd (cattle farm) choosing is won from Shuangcheng area of Harbin City Rong Sen animal husbandry (cattle farm), Shuangcheng area of Harbin City
Select experimental animal.
2. materials and methods
2.1 experimental animal
Randomly choose the experiment milk cow of Pass Test condition.
2.2 experiment milk cow inclusive criterias
Suffer from endometritis milk cow inclusive criteria：By detecting and observing, milk cow fever, loss of appetite, spirit are found
It is dispirited；Vaginal orifice has the viscous purulence abnormal secretion outflow of grey white when bending over, exerting duty, is mixed with blood sometimes and carries stink；Directly
Intestines check uterus enlargement, sensitiveness difference or have pain.
Suffer from infertility milk cow inclusive criteria：Continuous three oestrous cycles have no heat or have no obvious heat, the entanglement of feelings phase
Or after heat by three oestrous cycles breeding but fail to become pregnant.
Suffer from the inclusive criteria of retention of afterbirth milk cow：Milk cow postpartum 12h afterbirths are not completely exhausted out.
2.3 trial drug
The filling liquid of the preparation of embodiment 1, the perfusion liquid of the preparation of comparative example 1~5, the rich imperial Ursocycline injection of Australia.
2.4 experiment packets and processing
It is random to select 70 milk cows for suffering from slight endometritis, and 10 milk cows for suffering from severe endometritis, wherein
70 milk cows for suffering from slight endometritises are randomly divided into 7 groups, every group 10, wherein 6 groups of milk for suffering from slight endometritis
The ox perfusion liquid that respectively prepared by one-to-one perfusion embodiment 1 and comparative example 1~5, the milk cow with severe endometritis
Irrigate perfusion liquid prepared by embodiment 1；The usage and dosage of perfusion liquid prepared by embodiment 1 and comparative example 1~5 for 100ml/ times/
My god, it is used in conjunction 3 days；The rich imperial Ursocycline injection of remaining one group of perfusion Australia carries out uterine perfusion, and dosage is 100ml/ times/day, is used
Middle ware is every two days twice for medicine；
It is random to select 70 milk cows for suffering from infertility, 7 groups are randomly divided into, respectively the one-to-one perfusion He of embodiment 1
Perfusion liquid prepared by comparative example 1~5, usage and dosage are 100ml/ times/day, are used in conjunction 3 days；The rich dragon soil of remaining one group of perfusion Australia is mould
Plain parenteral solution carries out uterine perfusion, and dosage is 100ml/ times/day, and middle ware is every two days twice for medication；
70 milk cows for suffering from postpartum retention of afterbirth are selected at random, are randomly divided into 7 groups, wherein 6 groups of one-to-one fillings respectively
Perfusion liquid prepared by embodiment 1 and comparative example 1~5 is noted, usage and dosage is 100ml/ times/day, is used in conjunction 3 days；Remaining one group of filling
Note the rich imperial Ursocycline injection of Australia and carry out uterine perfusion, dosage is 100ml/ times/day, and middle ware is every two days twice for medication.
2.5 therapeutic effects are evaluated
Therapeutic effect is divided into healing, invalid two kinds：
Cure：Cow endometritis rehabilitation standard：Inflammation of uterus disappears after medication, and it is not swollen that palpation suffers from cattle uterus
Block, have no pain reaction, intrauterine secretion discharge without exception, hence it is evident that heat, become pregnant after artificial insemination within 3 feelings phases.
Barrenness of milk cow rehabilitation standard：The normal heat of milk cow after medication, become pregnant after artificial insemination within 3 feelings phases.
Milk cow retention of afterbirth rehabilitation standard：After medication, milk cow afterbirth is completely exhausted out.
It is invalid：Uterus symptom changes without obvious after medication, without heat phenomenon within 3 feelings phases.
3. result of the test.
The rich dragon of perfusion liquid and Australia prepared by the embodiment 1 of table 1 and comparative example 1~5 is treated to treatment cow endometritis clinic
Effect statistics
The clinical efficacy of the rich dragon of perfusion liquid and Australia prepared by the embodiment 1 of table 2 and comparative example 1~5 to treatment barrenness of milk cow
Statistics
| Group | Embodiment 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 | The rich dragon of Australia |
| Conception rate | 76.92% | 45% | 42% | 40% | 42% | 2% | 40% |
The rich dragon of perfusion liquid and Australia prepared by the embodiment 1 of table 3 and comparative example 1~5 is treated to the clinical for the treatment of milk cow retention of afterbirth
Effect statistics
| Group | Embodiment 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 | The rich dragon of Australia |
| It is efficient | 100% | 40% | 45% | 25% | 52% | 2% | 50% |
4. interpretation of result
From the data shown in above-mentioned table 1, table 2 and table 3, perfusion liquid prepared by the embodiment of the present invention 1 is slight to milk cow
Significant therapeutic effect is respectively provided with illnesss such as severe endometritis, infertility and retentions of afterbirth, and therapeutic effect is obvious
Better than comparative example 1~5, and combine the clinicing aspect progress such as uterus recovery situation, ovarian activity, corpus luteum and heat, fertilization
Analysis, the curative effect of medication of perfusion liquid prepared by the embodiment of the present invention 1 are superior to comparative example 1~5, it can be seen that the present invention uses day
Sbombycin antibacterial peptide is combined main preparing raw material of the traditional Chinese medicinal components as perfusion liquid with bacillus subtilis so that is produced between each other
Synergistic function, lift the curative effect of medication of perfusion liquid.Perfusion liquid level pure Chinese medicine green product prepared by the present invention, safely, is controlled
Therapeutic effect is obvious, in the absence of medicament residue problem.
5. conclusion
The present invention promotes the sexual maturity progestogenic perfusion liquid by 100ml/ times/day, is used in conjunction 3 days, 3 next course for the treatment of, treats in cow uteri
Film inflammation, infertility, postpartum retention of afterbirth therapeutic effect are obvious.
Test example 2
1. test site
The gloomy animal husbandry of Harbin City's rising sun
2. test material and method
2.1 experimental animal
Experiment milk cow is randomly choosed according to test requirements document.
2.2 Experimental agents
Perfusion liquid prepared by embodiment 1；Perfusion liquid prepared by comparative example 3；Strength palace health (24g/ props up chlorhexidine containing acetic acid),
Shanxi Ruicheng Wei Erfu veterinary drugs Co., Ltd provides, product batch number 0812284, palace absolute oil (herbal mixture, 200ml/ bottles), Hangzhou
Singapore dollar enjoys pharmaceutcal corporation, Ltd's offer, product batch number：0902294.
2.3 experiment milk cow inclusive criterias
Suffer from endometritis milk cow inclusive criteria：By detecting and observing, milk cow fever, loss of appetite, spirit are found
It is dispirited；Vaginal orifice has the viscous purulence abnormal secretion outflow of grey white when bending over, exerting duty, is mixed with blood sometimes and carries stink；Directly
Intestines check uterus enlargement, sensitiveness difference or have pain.
Suffer from infertility milk cow inclusive criteria：Continuous three oestrous cycles have no heat or have no obvious heat, the entanglement of feelings phase
Or after heat by three oestrous cycles breeding but fail to become pregnant.
Suffer from the inclusive criteria of retention of afterbirth milk cow：Milk cow postpartum 12h afterbirths are not completely exhausted out.
3. experiment packet and processing
3.1 suffer from light, severe endometritis milk cow clinical test packet
40 milk cows for suffering from slight endometritis of random selection, are randomly divided into 4 groups, every 10 first groups, every group one-to-one
Perfusion liquid, strength palace health and palace absolute oil prepared by perfusion liquid, the comparative example 3 prepared to cow uteri perfusion embodiment 1；Wherein implement
The usage and dosage of perfusion liquid prepared by example 1 and comparative example 3 is 100ml/ times/day, and 3 days are a course for the treatment of；The usage and dosage of strength palace health
For one every time, interval once irrigates 4 times altogether on the 2nd；The usage and dosage of uterine neck oil is 100ml/ times/day, and 3 days are a course for the treatment of；
Randomly choose 40 milk cows for suffering from severe endometritis, be randomly divided into 4 groups, every 10 first groups, every group one a pair
Perfusion liquid, strength palace health and palace absolute oil prepared by the perfusion liquid, the comparative example 3 that are prepared to cow uteri perfusion embodiment 1 answered；Its
The usage and dosage of perfusion liquid prepared by middle embodiment 1 and comparative example 3 is 100ml/ times/day, and 3 days are a course for the treatment of；Strength palace health
Usage and dosage be every time one, interval once irrigates 3 times altogether on the 2nd；The usage and dosage of uterine neck oil is 100ml/ times/day, 3 days
For a course for the treatment of；
3.2 infertile milk cow clinical test packets
80 Repeat breeding milk cows are randomly choosed, are randomly divided into 4 groups, every group 20, every group correspondingly to repeatly with not
The pregnant milk cow perfusion liquid that before experiment milk cow breeding prepared by uterine perfusion embodiment 1, the perfusion liquid of the preparation of comparative example 3, strength palace
Health and palace absolute oil；Wherein the usage and dosage of perfusion liquid prepared by embodiment 1 and comparative example 3 is 100ml/ times/day, and 3 days are one
The course for the treatment of；The usage and dosage of strength palace health is one every time, and interval once irrigates 4 times altogether on the 2nd；Uterine neck oil usage and dosage be
100ml/ times/day, 3 days are a course for the treatment of；
The retention of afterbirth milk cow clinical test of 3.3 postpartum is grouped
80 postpartum retention of afterbirth milk cows are randomly choosed, are randomly divided into 4 groups, every group irrigates cow uteri correspondingly
Perfusion liquid, strength palace health and palace absolute oil prepared by perfusion liquid, the comparative example 3 of the preparation of embodiment 1；Wherein embodiment 1 and comparative example 3
The usage and dosage of the perfusion liquid of preparation is 100ml/ times/day, and 3 days are a course for the treatment of；The usage and dosage of strength palace health is every time one
Branch, interval once irrigate 4 times altogether on the 2nd；The usage and dosage of uterine neck oil is 100ml/ times/day, and 3 days are a course for the treatment of.
4. treatment results are evaluated
Therapeutic effect is divided into healing, invalid two kinds.
Cure：Cow endometritis rehabilitation standard：Inflammation of uterus disappears after medication, it is not swollen that palpation suffers from cattle uterus
Block, have no pain reaction, intrauterine secretion discharge without exception, hence it is evident that heat, become pregnant after artificial insemination within 3 feelings phases.
Barrenness of milk cow rehabilitation standard：The normal heat of milk cow after medication, become pregnant after artificial insemination within 3 feelings phases.
Milk cow retention of afterbirth rehabilitation standard：After medication, milk cow afterbirth is completely exhausted out.
It is invalid：Uterus symptom changes without obvious after medication, without heat phenomenon within 3 feelings phases.
5. result of the test
The clinical efficacy statistics of the different drug treatment cow endometritis of table 4
The clinical efficacy statistics of the different drug treatment barrenness of milk cow of table 5
The clinical efficacy statistics of the different drug treatment milk cow postpartum retentions of afterbirth of table 6
6th, result of the test
From the data shown in above-mentioned table 4~6, perfusion liquid prepared by the embodiment of the present invention 1 to cow endometritis,
The curative effect of the reproductive diseases such as infertility and retention of afterbirth be superior to comparative example 3 preparation pure Chinese medicine perfusion liquid, and by
In commercially available strength palace health and palace absolute oil, from the clinicing aspect such as uterus recovery situation, ovarian activity, corpus luteum and heat, fertilization
Analyzed, the clinical effectiveness of perfusion liquid prepared by embodiment 1 is more preferably.
7th, conclusion
Perfusion liquid of the present invention adds cecropin antimicrobial peptides, bacillus subtilis, can repair cow uteri inner film injury, balances
Endometrium microorganism species, cecropin antimicrobial peptides are to Main Pathogenic Bacteria salmonella, Escherichia coli, staphylococcus aureus etc.
12 kinds of germs have very strong bactericidal action, and perfusion liquid of the present invention is using Chinese herbal medicine and cecropin antimicrobial peptides, bacillus subtilis
Double treatment, mutual Synergistic, the therapeutic effect to illnesss such as cow endometritis, infertility and retentions of afterbirth is lifted,
And pure natural raw material is used, antibiotic-free residual is safe and efficient.
Test example 3
To verify perfusion liquid of the present invention to Gilt Uterus intimitis, the therapeutic effect of infertility, we are in Luoyang City Luanchuan
County Green City Mountain ecologic breeding garden has carried out clinical test, and progestogenic perfusion liquid of the present invention is divided into high and low two groups by pharmaceutical quantities difference
Comparison of therapeutic is carried out, to determine perfusion liquid treatment Gilt Uterus intimitis, the therapeutic effect of infertility and curative effect of the present invention.
1. materials and methods
1.1 experimental animal
Endometritis, infertility sow are suffered from selection.
1.2 trial drug
Perfusion liquid prepared by embodiment 1.
1.3 experiment sow inclusive criterias
Suffer from endometritis sow inclusive criteria：5-7 days sow postpartum, sow vulva flow out dense white, bois de rose, yellowish-white
Colour band has bad smell mucus or purulent secretion.
Suffer from infertility milk cow inclusive criteria：Continuous three oestrous cycles have no heat or have no obvious heat, the entanglement of feelings phase
Or after heat by three oestrous cycles breeding but fail to become pregnant.
1.4 experiment packets and processing
Randomly select 40 sows for suffering from severe endometritis, be randomly divided into two groups, every group 20, one of which according to
100ml/ times/day of dosage carries out uterine perfusion to sow, is used in conjunction 3 days；Another set is according to 50ml/ times/day of dosage to mother
Pig carries out uterine perfusion, is used in conjunction 3 days；
Randomly select 40 sows for suffering from slight endometritis, be randomly divided into two groups, every group 20, one of which according to
100ml/ times/day of dosage carries out uterine perfusion to sow, is used in conjunction 3 days；Another set is according to 50ml/ times/day of dosage to mother
Pig carries out uterine perfusion, is used in conjunction 3 days；
40 sows for suffering from infertility are randomly selected, are randomly divided into two groups, every group 20, one of which is according to 100ml/
Times/day dosage to sow carry out uterine perfusion, be used in conjunction 3 days；Another set is carried out according to 50ml/ times/day of dosage to sow
Uterine perfusion, it is used in conjunction 3 days.
Note：In order to make medicine fully be contacted with endometrium during experiment, low dose group using 50ml medicines with
50ml0.9% physiological saline is used cooperatively.
1.5 therapeutic effects are evaluated
Therapeutic effect is divided into recovery from illness, invalid two kinds.
Cure：Endometritis recovery from illness standard：Lochia is disappeared, and uterus is recovered normally as recovery from illness；Lochia amount is reduced, viscosity
Reduce, in pale or egg white sample lochia occur be effective；Clinical symptoms do not mitigate, and the amount of lochia is not reduced or increased as nothing
Effect.
Infertility recovery from illness standard：Oestrus of sow is normal, and can be become pregnant after artificial insemination.
It is invalid：Clinical symptoms do not mitigate, and the amount of lochia does not decrease or increase, without normal heat.
2. result of the test
Perfusion liquid high and low dose treatment Gilt Uterus intimitis clinical efficacy statistics prepared by the embodiment 1 of table 7
Perfusion liquid high and low dose treatment Gilt Uterus intimitis clinical efficacy statistics prepared by the embodiment 1 of table 8
4th, interpretation of result
Perfusion liquid high and low dose group treatment Gilt Uterus inner membrance of the present invention is understood as the data shown in above-mentioned table 7 and table 8
Scorching, infertility significant effect, is analyzed, progestogenic perfusion liquid of promoting the sexual maturity from the clinicing aspect such as uterine secretion thing and heat, fertilization
The treatment of high and low two kinds of dosage can reach obvious therapeutic effect.
5th, conclusion
Perfusion liquid of the present invention presses 100ml/ times/day, is used in conjunction 3 days, 3 times are a course for the treatment of, progestogenic perfusion liquid of promoting the sexual maturity 50ml+
50ml0.9% physiological saline/times/day, it is used in conjunction 3 days；Two kinds of dosages make to treatment Gilt Uterus intimitis, infertility clinic
Use significant effect.
Test example 4
In order to verify perfusion liquid of the present invention to Gilt Uterus intimitis, the therapeutic effect of infertility, in Nanyang Xinye County
Spark plant has carried out clinical test, perfusion liquid prepared by perfusion liquid, comparative example 3 prepared by the embodiment of the present invention 1 according to
Two groups of curative effects of high and low dose carry out Comparison of clinical effect with the market like product, to determine perfusion liquid treatment sow of the present invention
Endometritis, the clinical effectiveness of infertility and curative effect.
1 materials and methods
1.1 experimental animal
Selection meets the experiment sow of experimental condition
1.2 test drug
Perfusion liquid prepared by test drug embodiment 1；Perfusion liquid prepared by comparative example 3；GONGYANJING, by Hunan agricultural university animal
Medicine company provides；Commercially available progestogenic perfusion liquid, Lay medicine company is won by Jiangxi and provided.
1.3 experiment sow inclusive criterias
Suffer from endometritis sow inclusive criteria：5-7 days sow postpartum, sow vulva flow out dense white, bois de rose, yellowish-white
Colour band has bad smell mucus or purulent secretion.
Suffer from infertility milk cow inclusive criteria：Continuous three oestrous cycles have no heat or have no obvious heat, the entanglement of feelings phase
Or after heat by three oestrous cycles breeding but fail to become pregnant.
1.4 experiment packets and processing
1.41 suffer from light, severe endometritis sow clinical test packet
Slight endometritis sows are suffered from random selection 80, are randomly divided into 4 groups, every 20 first groups, every group of one-to-one corresponding
Perfusion embodiment 1 prepare perfusion liquid, comparative example 3 prepare perfusion liquid, GONGYANJING and commercially available progestogenic perfusion liquid；Embodiment 1
Perfusion liquid, the perfusion liquid of the preparation of comparative example 3 and the usage and dosage of commercially available progestogenic perfusion liquid of preparation are that Gilt Uterus is carried out
Perfusion, is used in conjunction 3 days by 100ml/ times/day；GONGYANJING carries out uterine perfusion to experiment sow, 100ml/ times/day, is spaced two days 1
It is secondary, medication 3 times；
Severe endometritis sow is suffered from random selection 80, is randomly divided into 4 groups, every 20 first groups, every group of one-to-one corresponding
Perfusion embodiment 1 prepare perfusion liquid, comparative example 3 prepare perfusion liquid, GONGYANJING and commercially available progestogenic perfusion liquid；Embodiment 1
Perfusion liquid, the perfusion liquid of the preparation of comparative example 3 and the usage and dosage of commercially available progestogenic perfusion liquid of preparation are that Gilt Uterus is carried out
Perfusion, is used in conjunction 3 days by 50ml/ times/day；GONGYANJING carries out uterine perfusion to experiment sow, 50ml/ times/day, is spaced 1 time on the two,
Medication 3 times；
1.42 suffer from infertility sow clinical test packet
Infertility sow is suffered from random selection 80, is randomly divided into 4 groups, every 20 first groups, every group irrigates reality correspondingly
Apply the perfusion liquid of the preparation of example 1, perfusion liquid, GONGYANJING and commercially available progestogenic perfusion liquid prepared by comparative example 3；Filling prepared by embodiment 1
The usage and dosage of fluid injection, perfusion liquid prepared by comparative example 3 and commercially available progestogenic perfusion liquid is that Gilt Uterus is irrigated,
100ml/ times/day, it is used in conjunction 3 days；GONGYANJING carries out uterine perfusion to experiment sow, 100ml/ times/day, is spaced 1 time on the two, medication
3 times；
Infertility sow is suffered from random selection 80, is randomly divided into 4 groups, every 20 first groups, every group irrigates reality correspondingly
Apply the perfusion liquid of the preparation of example 1, perfusion liquid, GONGYANJING and commercially available progestogenic perfusion liquid prepared by comparative example 3；Filling prepared by embodiment 1
The usage and dosage of fluid injection, perfusion liquid prepared by comparative example 3 and commercially available progestogenic perfusion liquid is to be irrigated 50ml/ to Gilt Uterus
Times/day, it is used in conjunction 3 days；GONGYANJING carries out uterine perfusion to experiment sow, 50ml/ times/day, is spaced 1 time on the two, medication 3 times.
Note：In order to make medicine fully be contacted with endometrium during experiment, low dose group using 50ml medicines with
50ml0.9% physiological saline is used cooperatively
2. test effect is evaluated
Therapeutic effect is divided into recovery from illness, invalid two kinds.
Cure：Endometritis recovery from illness standard：Lochia is disappeared, and uterus is recovered normally as recovery from illness；Lochia amount is reduced, viscosity
Reduce, in pale or egg white sample lochia occur be effective；Clinical symptoms do not mitigate, and the amount of lochia is not reduced or increased as nothing
Effect.
Infertility recovery from illness standard：Oestrus of sow is normal, and can be become pregnant after artificial insemination.
It is invalid：Clinical symptoms do not mitigate, and the amount of lochia does not decrease or increase, without normal heat.
3. result of the test
The high dose difference drug treatment Gilt Uterus intimitis clinical efficacy of table 9 counts
The low dosage difference drug treatment Gilt Uterus intimitis clinical efficacy of table 10 counts
The high dose difference drug treatment sow infertility clinical efficacy of table 11 counts
The low dosage difference drug treatment sow infertility clinical efficacy of table 12 counts
4th, result
The perfusion liquid high and low dose group prepared as the as shown by data embodiment of the present invention 1 shown in above-mentioned table 9~12 is treated female
Pig endometritis, infertility significant effect, analyze, and combined to disease from clinicing aspects such as uterine secretion thing, heat, fertilizations
Treated effect, the embodiment of the present invention 1 prepare perfusion liquid therapeutic effect be substantially better than comparative example 3 preparation pure Chinese medicine fill
Fluid injection and commercially available progestogenic perfusion liquid, and commercially available GONGYANJING class medicine,
5th, analyze
1 perfusion liquid of the embodiment of the present invention, two kinds of 100ml/ times/day of dosages, 50ml/ times/day for the treatment of Gilt Uterus inner membrance
Scorching, postpartum retention of afterbirth, research have shown that perfusion liquid treatment sow reproductive diseases significant effect of the present invention, good effect, short treating period, province
Shi Shengli, clinical effectiveness are superior to reference product；Show to add cecropin antimicrobial peptides, bacillus subtilis in perfusion liquid of the present invention
Bacterium, endometrial impairment can be repaired, balance endometrium microorganism species, cecropin antimicrobial peptides are to Main Pathogenic Bacteria Salmonella
12 kinds of germs such as bacterium, Escherichia coli, staphylococcus aureus have very strong bactericidal action；Using Chinese herbal medicine and cecropin antibacterial
Peptide, bacillus subtilis double treatment, Synergistic, lifting medicine is to therapeutic effect.
Note：Experiment proves that perfusion liquid prepared by embodiment 2 and embodiment 3 is to dam endometritis, infertility and tire
The therapeutic effect for the perfusion liquid that the therapeutic effect of the not inferior illness of clothing is prepared with embodiment 1 is substantially equivalent.
Claims (7)
1. a kind of Chinese medicine composition for improving dam fecundity, it is characterised in that mainly by the raw material system of following parts by weight
It is standby to form：5~10 parts of cecropin antimicrobial peptides, 10~20 parts of barrenwort, 15~20 parts of motherwort, 8~15 parts of safflower, the red sage root 8~
15 parts, 15~20 parts of the red meals of sheep, 5~15 parts of honeysuckle, 2~5 parts of bacillus subtilis.
2. the Chinese medicine composition of dam fecundity is improved as claimed in claim 1, it is characterised in that the Chinese medicine composition
Formulation be perfusion liquid；The raw material of following parts by weight is further comprises in the perfusion liquid preparation process：10~20 parts of glycerine, gather
1~5 part of vinylpyrrolidone, 0.15~0.5 part of preservative, 0.01~0.1 part of antioxidant.
3. a kind of preparation method of the Chinese medicine composition as claimed in claim 2 for improving dam fecundity, including following behaviour
Make step：
1) effective component of chinese medicine extract is extracted：Red meals of barrenwort, motherwort, safflower, the red sage root, sheep and double are weighed in parts by weight
Crushed after flower mixing, with supercritical CO2Fluid is extractant, is extracted using the mixed solvent of water, ethanol and acetone as entrainer
Take, decompression separation is carried out after extraction, discharge, obtain effective component of chinese medicine extract；
2) perfusion liquid is prepared：The effective component of chinese medicine for taking step 1) to prepare, add cecropin antimicrobial peptides, bacillus subtilis, sweet
After oil, polyvinylpyrrolidone, preservative and antioxidant are well mixed, distilled water constant volume is added, obtains perfusion liquid.
4. the preparation method of the Chinese medicine composition of dam fecundity is improved as claimed in claim 3, it is characterised in that step
1) specific method of extraction effective component of chinese medicine is in：
A：The red meals of barrenwort, motherwort, safflower, the red sage root, sheep and honeysuckle are weighed in parts by weight, and extractor is added after co-grinding
In, supercritical CO is passed through in extractor2Fluid and entrainer, under 30~40MPa pressure, 40~75 DEG C of temperature conditionss, extraction
Take 3~5h；
B：Successively by primary separator, second-stage separator after the completion of step A extractions, by effective component of chinese medicine and supercritical CO2Stream
Body is separated, wherein supercritical CO2Fluid returns to be recycled in extractor, and primary separator discharging is big for a small amount of toughness
Traditional Chinese medicinal ointment, detect no effective component of chinese medicine, second-stage separator discharging is the effective component of chinese medicine extract of brown, and splitter goes out
Expect the entrainer liquid for clarification；Wherein supercritical CO2Fluid flow through primary separator carry out first separation pressure for 30MPa,
Temperature is 50~75 DEG C；The pressure for flowing through second-stage separator progress secondary separation is 30MPa, temperature is 35 DEG C.
5. the preparation method of the Chinese medicine composition of the raising dam fecundity as described in claim 3 or 4, it is characterised in that
The entrainer is water, ethanol, acetone with volume ratio 1：4~7:2~5 mixed solution.
6. the preparation method of the Chinese medicine composition of dam fecundity is improved as claimed in claim 5, it is characterised in that described
The quality dosage of entrainer is the 5~20% of barrenwort, motherwort, safflower, the red sage root, the red meals of sheep and honeysuckle quality total amount.
7. the preparation method of the Chinese medicine composition of the raising dam fecundity as described in claim 3 or 4, it is characterised in that
Supercritical CO2The flow of fluid is 15~30L/h.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105535125A (en) * | 2016-01-08 | 2016-05-04 | 牙俊福 | Medicine for treating retained fetal membrane of pig |
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| CN1544004A (en) * | 2003-11-13 | 2004-11-10 | 南京农业大学 | Chinese traditional medicine perfusate for preventing and treating female livestock barrenness |
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2017
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1544004A (en) * | 2003-11-13 | 2004-11-10 | 南京农业大学 | Chinese traditional medicine perfusate for preventing and treating female livestock barrenness |
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| EUNJUNG LEE等: "Anti-inflammatory Activities of Cecropin A and Its Mechanism of Action", 《ARCH INSECT BIOCHEM PHYSIOL》 * |
| 李波等: "日粮中添加天蚕素抗菌肽对母猪繁殖性能的影响", 《中国畜牧兽医》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105535125A (en) * | 2016-01-08 | 2016-05-04 | 牙俊福 | Medicine for treating retained fetal membrane of pig |
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