CN107494343A - 一种泥鳅的人工繁殖方法 - Google Patents
一种泥鳅的人工繁殖方法 Download PDFInfo
- Publication number
- CN107494343A CN107494343A CN201710642431.1A CN201710642431A CN107494343A CN 107494343 A CN107494343 A CN 107494343A CN 201710642431 A CN201710642431 A CN 201710642431A CN 107494343 A CN107494343 A CN 107494343A
- Authority
- CN
- China
- Prior art keywords
- loach
- parts
- water
- pond
- close
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000252185 Cobitidae Species 0.000 title claims abstract description 205
- 238000000034 method Methods 0.000 title claims abstract description 43
- 230000003014 reinforcing effect Effects 0.000 claims abstract description 31
- 241000595940 Notostraca Species 0.000 claims abstract description 25
- 210000003608 fece Anatomy 0.000 claims abstract description 11
- 239000004615 ingredient Substances 0.000 claims abstract description 11
- 239000010871 livestock manure Substances 0.000 claims abstract description 11
- 241000411851 herbal medicine Species 0.000 claims abstract description 9
- 238000011534 incubation Methods 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 115
- 229940090044 injection Drugs 0.000 claims description 34
- 238000002347 injection Methods 0.000 claims description 34
- 239000007924 injection Substances 0.000 claims description 34
- 241000251468 Actinopterygii Species 0.000 claims description 23
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 18
- 102000002322 Egg Proteins Human genes 0.000 claims description 18
- 108010000912 Egg Proteins Proteins 0.000 claims description 18
- 210000004681 ovum Anatomy 0.000 claims description 18
- 230000037396 body weight Effects 0.000 claims description 15
- 240000007594 Oryza sativa Species 0.000 claims description 13
- 235000007164 Oryza sativa Nutrition 0.000 claims description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 13
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 13
- 235000009566 rice Nutrition 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- 210000001015 abdomen Anatomy 0.000 claims description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 12
- XNOPRXBHLZRZKH-MQYCRUOZSA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1C(CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-MQYCRUOZSA-N 0.000 claims description 11
- 229940082408 oxytocin injection Drugs 0.000 claims description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- 235000013339 cereals Nutrition 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 9
- 230000001488 breeding effect Effects 0.000 claims description 9
- 239000000292 calcium oxide Substances 0.000 claims description 9
- 235000012255 calcium oxide Nutrition 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 241000195493 Cryptophyta Species 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 239000004310 lactic acid Substances 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 6
- 239000006041 probiotic Substances 0.000 claims description 6
- 235000018291 probiotics Nutrition 0.000 claims description 6
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 5
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 5
- 241001313855 Bletilla Species 0.000 claims description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 5
- 241001016310 Epimedium grandiflorum Species 0.000 claims description 5
- 108010028690 Fish Proteins Proteins 0.000 claims description 5
- 235000019733 Fish meal Nutrition 0.000 claims description 5
- 244000207740 Lemna minor Species 0.000 claims description 5
- 235000006439 Lemna minor Nutrition 0.000 claims description 5
- 241000361919 Metaphire sieboldi Species 0.000 claims description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 5
- 235000001855 Portulaca oleracea Nutrition 0.000 claims description 5
- 241000405414 Rehmannia Species 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 241000124033 Salix Species 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- 229930003268 Vitamin C Natural products 0.000 claims description 5
- 238000001467 acupuncture Methods 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- 235000018905 epimedium Nutrition 0.000 claims description 5
- 239000004467 fishmeal Substances 0.000 claims description 5
- 239000000787 lecithin Substances 0.000 claims description 5
- 229940067606 lecithin Drugs 0.000 claims description 5
- 235000010445 lecithin Nutrition 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 230000000243 photosynthetic effect Effects 0.000 claims description 5
- 210000002826 placenta Anatomy 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 210000000115 thoracic cavity Anatomy 0.000 claims description 5
- 235000019154 vitamin C Nutrition 0.000 claims description 5
- 239000011718 vitamin C Substances 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 4
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 4
- 241000096284 Gynochthodes officinalis Species 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 4
- 239000007844 bleaching agent Substances 0.000 claims description 4
- 230000036449 good health Effects 0.000 claims description 4
- 239000002502 liposome Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 235000013557 nattō Nutrition 0.000 claims description 4
- 244000144977 poultry Species 0.000 claims description 4
- 239000003531 protein hydrolysate Substances 0.000 claims description 4
- 230000001988 toxicity Effects 0.000 claims description 4
- 231100000419 toxicity Toxicity 0.000 claims description 4
- 244000025254 Cannabis sativa Species 0.000 claims description 3
- 241000252234 Hypophthalmichthys nobilis Species 0.000 claims description 3
- 241000755266 Kathetostoma giganteum Species 0.000 claims description 3
- 239000001506 calcium phosphate Substances 0.000 claims description 3
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 3
- 235000011010 calcium phosphates Nutrition 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical class C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 claims description 3
- 230000002262 irrigation Effects 0.000 claims description 3
- 238000003973 irrigation Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 239000002434 gonadorelin derivative Substances 0.000 claims description 2
- 230000000474 nursing effect Effects 0.000 claims 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 150000002576 ketones Chemical class 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 150000003722 vitamin derivatives Chemical class 0.000 claims 1
- 230000012447 hatching Effects 0.000 abstract description 14
- 230000004083 survival effect Effects 0.000 abstract description 13
- 210000004907 gland Anatomy 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 230000001568 sexual effect Effects 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 230000035800 maturation Effects 0.000 abstract description 5
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 2
- 230000018109 developmental process Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000007913 intrathecal administration Methods 0.000 description 7
- 230000016087 ovulation Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000000384 rearing effect Effects 0.000 description 5
- 206010002091 Anaesthesia Diseases 0.000 description 4
- 230000037005 anaesthesia Effects 0.000 description 4
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 3
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 3
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005728 strengthening Methods 0.000 description 3
- 235000019155 vitamin A Nutrition 0.000 description 3
- 239000011719 vitamin A Substances 0.000 description 3
- 229940045997 vitamin a Drugs 0.000 description 3
- KJGNDQCYBNBXDA-GUBZILKMSA-N Arg-Arg-Cys Chemical compound C(C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CS)C(=O)O)N)CN=C(N)N KJGNDQCYBNBXDA-GUBZILKMSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 241001412627 Adenophora liliifolia Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241001443588 Cottus gobio Species 0.000 description 1
- SZQCDCKIGWQAQN-FXQIFTODSA-N Cys-Arg-Ala Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(O)=O SZQCDCKIGWQAQN-FXQIFTODSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- KRRMJKMGWWXWDW-STQMWFEESA-N Gly-Arg-Phe Chemical compound NC(=N)NCCC[C@H](NC(=O)CN)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 KRRMJKMGWWXWDW-STQMWFEESA-N 0.000 description 1
- AWTDTFXPVCTHAK-BJDJZHNGSA-N Ile-Cys-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)O)N AWTDTFXPVCTHAK-BJDJZHNGSA-N 0.000 description 1
- SITWEMZOJNKJCH-UHFFFAOYSA-N L-alanine-L-arginine Natural products CC(N)C(=O)NC(C(O)=O)CCCNC(N)=N SITWEMZOJNKJCH-UHFFFAOYSA-N 0.000 description 1
- ZTPWXNOOKAXPPE-DCAQKATOSA-N Lys-Arg-Cys Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CS)C(=O)O)N ZTPWXNOOKAXPPE-DCAQKATOSA-N 0.000 description 1
- WQDKIVRHTQYJSN-DCAQKATOSA-N Lys-Ser-Arg Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)N WQDKIVRHTQYJSN-DCAQKATOSA-N 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000223785 Paramecium Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- JJKSSJVYOVRJMZ-FXQIFTODSA-N Ser-Arg-Cys Chemical compound C(C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CO)N)CN=C(N)N JJKSSJVYOVRJMZ-FXQIFTODSA-N 0.000 description 1
- KCFKKAQKRZBWJB-ZLUOBGJFSA-N Ser-Cys-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(O)=O KCFKKAQKRZBWJB-ZLUOBGJFSA-N 0.000 description 1
- 101000857870 Squalus acanthias Gonadoliberin Proteins 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 241001482311 Trionychidae Species 0.000 description 1
- OKDNSNWJEXAMSU-IRXDYDNUSA-N Tyr-Phe-Gly Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)NCC(O)=O)C1=CC=C(O)C=C1 OKDNSNWJEXAMSU-IRXDYDNUSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 108010016616 cysteinylglycine Proteins 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- FSXRLASFHBWESK-UHFFFAOYSA-N dipeptide phenylalanyl-tyrosine Natural products C=1C=C(O)C=CC=1CC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FSXRLASFHBWESK-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 108010015792 glycyllysine Proteins 0.000 description 1
- 210000000003 hoof Anatomy 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
- A61K36/296—Epimedium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/43—Cuscutaceae (Dodder family), e.g. Cuscuta epithymum or greater dodder
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/746—Morinda
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8966—Fritillaria, e.g. checker lily or mission bells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Developmental Biology & Embryology (AREA)
- Inorganic Chemistry (AREA)
- Cell Biology (AREA)
- Environmental Sciences (AREA)
- Biochemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Sustainable Development (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Immunology (AREA)
- Virology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
Abstract
本发明公开了一种泥鳅的人工繁殖方法，包括：亲鳅培育池的整理、亲鳅强化、人工催产、孵化、育苗、放养。有益效果为：较低的密度和全面的营养条件有利于泥鳅性腺的成熟，中草药组合汤药有利于亲鳅发育成熟；强化后的丰年虫体内含有较高含量的多不饱和脂肪酸，适合作为泥鳅的开口饵料，投喂给泥鳅幼苗后有利于提高泥鳅幼体的免疫能力、应激能力、抗菌抗病能力，提高其成活率与生长发育速度；本发明方法操作简单、安全易控，并且使用发酵农家肥作为丰年虫强化饵料的主要原材料，不仅降低了成本，而且对环境友好，份发明方法的泥鳅幼苗的孵化率高、成活率高、幼体活力强，又适合工业化发展，因此具有较高的应用前景。
Description
技术领域
本发明涉及水产繁殖领域，尤其是涉及一种泥鳅的人工繁殖方法。
技术背景
泥鳅属鳅科，形体小，细长，只有三、四寸，且它体形圆，身短，皮下有小鳞片，颜色青黑，浑身沾满了自身的粘液，因而滑腻无法握住，前段略呈圆筒形，后部侧扁，腹部圆，头小、口小、下位，马蹄形，眼小，无眼下刺，须5对，鳞极其细小，圆形，埋于皮下，体背部及两侧灰黑色，全体有许多小的黑斑点，头部和各鳍上亦有许多黑色斑点，背鳍和尾鳍膜上的斑点排列成行，尾柄基部有一明显的黑斑，其他各鳍灰白色。泥鳅广泛分布于亚洲沿岸的中国、日本、朝鲜、俄罗斯及印度等地。在中国分布各地，南方分布较多，北方不常见。全年都可采收，夏季最多，泥鳅捕捉后，可鲜用或烘干用，可食用、入药，泥鳅被称为“水中之参”，生活在湖池，是营养价值很高的一种鱼，它和其它的鱼不相同，无论外表，体形，生活习性都不同，是一种特殊的鳅类。
泥鳅是近年来最为热门的养殖品种，其不仅营养价值可观，还具有一定的药用价值，所以市场需求量日益增加，养殖效益非常可观。我国传统养殖的泥鳅包括了泥鳅和大鳞副泥鳅，分属花鳅亚科中的泥鳅属和副泥鳅属。造成泥鳅价格居高不下的原因除了其本身品质较高外，还由于泥鳅养殖业产业链的不完善。尽管泥鳅的规模化人工繁殖已经取得了成功，但其产业链在苗种培育这一阶段出现了短板，泥鳅在苗种培育阶段的成活率很低，无法形成一个完整、连续、充满活力的产业链条，这成为了限制泥鳅养殖业发展的瓶颈。
关于泥鳅的人工繁殖技术有很多方法，现有技术如授权公众号为
CN 104542398 B的中国发明专利，公开了一种泥鳅春秋双季繁殖方法，该发明方法介绍了春秋双季节的泥鳅的人工繁殖，但是其春季繁殖的亲鳅采用自然排卵，以蛋黄水和虾粉作为幼苗的开口饵料，秋季繁殖的亲鳅采用往池塘中施入绒毛膜促性腺激素的方法来催产，而后幼苗的开口饵料选择草履虫或稚鳖料，该方法中亲鳅的产卵率不高，受精卵质量不均衡，导致其孵化率不高，而且泥鳅幼苗的开口饵料无法起到提高幼苗免疫力、应激反应能力的目的，因此会导致幼苗的成活率下降，产量降低。
发明内容
本方法的目的在于提供一种利用幼苗开口饵料经过强化后的泥鳅的人工繁殖方法。
本发明所用微生物及其所购买企业为：光合细菌、枯草芽孢杆菌、纳豆芽孢杆菌、乳酸菌、酵母菌，沧州旺发生物技术研究所；双歧杆菌，陕西森弗天然制品有限公司。
本发明针对背景技术中提到的问题，采取的技术方案为：一种泥鳅的人工繁殖方法，包括：亲鳅培育池的整理、亲鳅强化、人工催产、孵化、育苗、放养，具体包括以下步骤：
亲鳅培育池的整理：选取靠近水源、无污染、水质优良、排灌方便、环境安静的地方搭建亲鳅培育池，池塘面积为0.5-3亩、池深为0.8-1.5米、水深0.4-0.8米，池底与四周铺上厚度为0.3-0.5mm的塑料薄膜，塑料薄膜延伸至池坝外侧，池底铺软腐泥20-30cm，进排水口都要蒙上70-90目的筛绢网；用生石灰80-150kg/亩进行干法消毒，进水10-25cm，再次施生石灰20-50kg/亩化水后全池泼洒，加水至0.4-0.8米后施用腐熟的农家肥50-90kg/亩肥水，待池水呈浅绿色时先用几尾泥鳅入池养殖2-3天测试毒性，无恙后方可大规模培育亲鳅；亲鳅培养期间，控制水温为15-17℃，pH为6.5-8.5，每隔10-15 天可使用50-100g益生菌作为微生态制剂向池内泼洒1次，以调节水质；对培育池进行消毒、肥水、试养，有利于亲鳅的强化培养；
亲鳅强化：选取雌泥鳅要腹部膨大，有弹性，将其肚皮向上，颜色微红用手触摸肋骨明显，体重40-60g，体长20-25cm；雄泥鳅选取标准为个体较大，体质健壮，活动能力强，体重35-50g,体长15-20cm；强化密度为200-250kg/亩，强化期间以蚯蚓10-15份、蝇蛆10-15份、禽畜下脚料20-30份、鱼粉5-10份、豆粕30-40份、麦麸30-40份、酵母粉5-10份、维生素C1-2份、维生素A1-2份、浮萍20-25份、碎菜叶30-40份混合均匀作为强化饲料，日投喂量为泥鳅体重的6-10%，每天投喂2-3次；取北沙参1-2份、川贝母1-2份、白及2-3份、紫河车1-2份、巴戟天2-3份、熟地黄3-5份、菟丝子2-3份、淫羊藿1-1.5份混合后放于水中煎煮，将得到的中草药组合汤药泼洒在亲鳅培育池中；较低的密度和全面的营养条件有利于泥鳅性腺的成熟，中草药组合汤药有利于亲鳅发育成熟，为人工催产做准备；
人工催产：以注射催产素的方法进行人工催产，注射之前采用MS-222进行麻醉，注射部位为胸腔，扎针2-3毫米，注射方向与体轴腹面成30-45°角，雌鳅注射药液0.15-0.2ml、雄鳅0.08-0.1ml，注射时可用湿纱布包住亲鳅露出注射部位进行注射；亲鳅注射催产素后，按雌雄比例1∶2-3放入悬挂在鱼池水中用40-60目筛绢制成的网箱内，箱中放养水草并用棕片或杨柳根须做成鱼巢，在25-28℃水温条件下，注射后的亲鳅经8-10小时便开始发情，表现为亲鳅在水中激烈翻腾，继而雄鳅卷住雌鳅身体产卵、排精，卵产于鱼巢上，产卵过程约20-30分钟，产卵后要及时取出粘有卵粒的鱼巢另池孵化，以防亲鳅吞食卵粒；先行麻醉后进行胸腔注射催产素可以促进亲鳅排卵排精，胸腔注射不会对亲鳅造成伤害；
孵化：将受精卵转移至微流水孵化箱中进行孵化，孵化箱水体溶氧6-10 mg/L，pH为7.0-7.5，水深0.4-0.5米，放卵密度为每升水体400-600粒，在水温为24-26℃时约30-35小时即可孵出鳅苗；在微流水孵化箱中进行孵化可以保证仔鳅的孵化率与成活率可达到90%以上，生产效率极高；
育苗：育苗池面积为1-2亩，池底平整无杂草，水深0.3-0.5米，进水口架设60-90目拦网，放苗前7-10天以15-20kg/亩漂白粉进行清池，然后进行浮网和试水操作；将鳅苗以30-40万尾/亩的密度进行培育，选择强化后的丰年虫作为幼体的开口饵料，丰年虫的强化饵料为裂壶藻10-15份、小球藻10-15份、卵磷脂脂质体20-25份、鱼内脏油20-25 份、鱼蛋白水解物28-33份、尿素20-23份、硝酸铵18-20份、磷酸钙15-20份、发酵农家肥60-80份、活性多肽1-2份充分混合均匀后粉碎成10-45微米大小的颗粒，强化喂养丰年虫8-10小时即可投喂给鳅苗；日投喂量为3-5kg/亩，幼苗长至3-5cm时育苗结束；活性多肽的氨基酸序列为：SCASRCKSRCRARRCRARRCGRFYFGKRCICKCFRC；活性多肽可以促进丰年虫对强化饵料的消化吸收，提高强化效率，经过强化后的丰年虫体内多不饱和脂肪酸的含量较高，适合作为泥鳅的开口饵料，该育苗条件有利于提高泥鳅幼体的免疫能力、应激能力、抗菌抗病能力，提高其成活率与生长发育速度；
放养：泥鳅幼苗长至3-5cm时即可售卖或者放养，放养密度为2-3.5万尾/亩，在泥鳅池中可适当搭养中上层鱼类，如草、鲢、鳙等夏花鱼种，放养时间在9-10月，养殖时间8-9个月。
作为优选，益生菌为光合细菌或枯草芽孢杆菌或纳豆芽孢杆菌或乳酸菌或酵母菌或双歧杆菌的至少3种混合菌群；益生菌有利于改善培育池的水质，促进亲鳅的生长发育。
作为优选，催产素为比例为2-3:2-3：100的促黄体素释放素类似物与绒毛膜促性腺激素与地欧酮；催产素有利于促进亲鳅排卵排精，提高产量。
与现有技术相比，本发明的优点在于：1）较低的密度和全面的营养条件有利于泥鳅性腺的成熟，中草药组合汤药有利于亲鳅发育成熟，先行麻醉后进行胸腔注射催产素可以促进亲鳅排卵排精，胸腔注射不会对亲鳅造成伤害；2）强化后的丰年虫体内含有较高含量的多不饱和脂肪酸，适合作为泥鳅的开口饵料，投喂给泥鳅幼苗后有利于提高泥鳅幼体的免疫能力、应激能力、抗菌抗病能力，提高其成活率与生长发育速度；3）本发明方法操作简单、安全易控，并且使用发酵农家肥作为丰年虫强化饵料的主要原材料，不仅降低了成本，而且对环境友好，份发明方法的泥鳅幼苗的孵化率高、成活率高、幼体活力强，又适合工业化发展，因此具有较高的应用前景。
具体实施方式
下面通过实施例对本发明方案作进一步说明：
实施例1：
一种泥鳅的人工繁殖方法，包括以下步骤：
1）搭建面积0.5亩、池深0.8米、水深0.4米的培育池，池底与四周铺上厚度为0.3mm的塑料薄膜，池底铺软腐泥20cm，进排水口都要蒙上70目的筛绢网；用生石灰80kg/亩进行干法消毒，进水10cm，再次施生石灰20kg/亩化水后全池泼洒，加水至0.4米后施用腐熟的农家肥50kg/亩肥水，待池水呈浅绿色时测试毒性；亲鳅培养期间，控制水温为15℃，pH为6.5，每隔10天可使用10g光合细菌、20g枯草芽孢杆菌、20g乳酸菌混合制剂向池内泼洒1次，以调节水质；对培育池进行消毒、肥水、试养，有利于亲鳅的强化培养；2）选取雌泥鳅体重40g，体长20cm，雄泥鳅体重35g,体长15cm；强化密度为200kg/亩，强化期间以蚯蚓10份、蝇蛆10份、禽畜下脚料20份、鱼粉5份、豆粕30份、麦麸30份、酵母粉5份、维生素C 1份、维生素A 1份、浮萍20份、碎菜叶30份混合均匀作为强化饲料，日投喂量为泥鳅体重的6%，每天投喂2次；另取北沙参1份、川贝母1份、白及2份、紫河车1份、巴戟天2份、熟地黄3份、菟丝子2份、淫羊藿1份混合后放于水中煎煮，将得到的中草药组合汤药泼洒在亲鳅培育池中；较低的密度和全面的营养条件有利于泥鳅性腺的成熟，中草药组合汤药有利于亲鳅发育成熟，为人工催产做准备；3）以注射催产素的方法进行人工催产，催产素为比例为2:2：100的促黄体素释放素类似物与绒毛膜促性腺激素与地欧酮，注射之前进行麻醉，注射部位为胸腔，扎针2毫米，注射方向与体轴腹面成30°角，雌鳅注射药液0.15ml、雄鳅0.08ml，注射时可用湿纱布包住亲鳅露出注射部位进行注射；亲鳅注射催产素后，按雌雄比例1∶2放入悬挂在鱼池水中用40目筛绢制成的网箱内，箱中放养水草并用棕片或杨柳根须做成鱼巢，在25℃水温条件下，注射后的亲鳅经8小时便开始发情，表现为亲鳅在水中激烈翻腾，继而雄鳅卷住雌鳅身体产卵、排精，卵产于鱼巢上，产卵过程约20分钟，产卵后要及时取出粘有卵粒的鱼巢另池孵化，以防亲鳅吞食卵粒；先行麻醉后进行胸腔注射催产素可以促进亲鳅排卵排精，胸腔注射不会对亲鳅造成伤害；4）将受精卵转移至微流水孵化箱中进行孵化，孵化箱水体溶氧6 mg/L，pH为7.0，水深0.4米，放卵密度为每升水体400粒，在水温为24℃时约30小时即可孵出鳅苗；在微流水孵化箱中进行孵化可以保证仔鳅的孵化率与成活率可达到90%以上，生产效率极高；5）育苗池面积为1亩，池底平整无杂草，水深0.3米，进水口架设60目拦网，放苗前7天以15kg/亩漂白粉进行清池，然后进行浮网和试水操作；将鳅苗以30万尾/亩的密度进行培育，选择强化后的丰年虫作为幼体的开口饵料，丰年虫的强化饵料为裂壶藻10份、小球藻10份、卵磷脂脂质体20份、鱼内脏油20份、鱼蛋白水解物28份、尿素20份、硝酸铵18份、磷酸钙15份、发酵农家肥60份、活性多肽1份充分混合均匀后粉碎成10微米大小的颗粒，强化喂养丰年虫8小时即可投喂给鳅苗；日投喂量为3kg/亩，幼苗长至3cm时育苗结束即可售卖；活性多肽的氨基酸序列为：SCASRCKSRCRARRCRARRCGRFYFGKRCICKCFRC；活性多肽可以促进丰年虫对强化饵料的消化吸收，提高强化效率。
实施例2：
一种泥鳅的人工繁殖方法，包括以下步骤：
1）亲鳅培育池的整理：搭建面积为3亩、池深为1.5米、水深0.8米的培育池，池底与四周铺上厚度为0.5mm的塑料薄膜，池底铺软腐泥30cm，进排水口都要蒙上90目的筛绢网；用生石灰150kg/亩进行干法消毒，进水25cm，再次施生石灰50kg/亩化水后全池泼洒，加水至0.8米后施用腐熟的农家肥90kg/亩肥水，待池水呈浅绿色时先用几尾泥鳅入池养殖3天测试毒性，无恙后方可大规模培育亲鳅；亲鳅培养期间，控制水温为17℃，pH为8.5，每隔15 天可使用20g光合细菌、20g枯草芽孢杆菌、20g纳豆芽孢杆菌、20g乳酸菌、20g酵母菌混合制剂作为微生态制剂向池内泼洒1次，以调节水质；对培育池进行消毒、肥水、试养，有利于亲鳅的强化培养；
2）亲鳅强化：选取雌泥鳅体重60g，体长25cm；选取雄泥鳅体重50g,体长20cm；强化密度为250kg/亩，强化期间以蚯蚓15份、蝇蛆15份、禽畜下脚料30份、鱼粉10份、豆粕40份、麦麸40份、酵母粉10份、维生素C 2份、维生素A 2份、浮萍25份、碎菜叶40份混合均匀作为强化饲料，日投喂量为泥鳅体重的10%，每天投喂3次；取北沙参2份、川贝母2份、白及3份、紫河车2份、巴戟天3份、熟地黄5份、菟丝子3份、淫羊藿1.5份混合后放于水中煎煮，将得到的中草药组合汤药泼洒在亲鳅培育池中；较低的密度和全面的营养条件有利于泥鳅性腺的成熟，中草药组合汤药有利于亲鳅发育成熟，为人工催产做准备；
3）人工催产：以注射催产素的方法进行人工催产，催产素为比例为3:3：100的促黄体素释放素类似物与绒毛膜促性腺激素与地欧酮，注射之前进行麻醉，注射部位为胸腔，扎针3毫米，注射方向与体轴腹面成45°角，雌鳅注射药液0.2ml、雄鳅0.1ml，注射时可用湿纱布包住亲鳅露出注射部位进行注射；亲鳅注射催产素后，按雌雄比例1∶3放入悬挂在鱼池水中用60目筛绢制成的网箱内，箱中放养水草并用棕片或杨柳根须做成鱼巢，在28℃水温条件下，注射后的亲鳅经10小时便开始发情，表现为亲鳅在水中激烈翻腾，继而雄鳅卷住雌鳅身体产卵、排精，卵产于鱼巢上，产卵过程约30分钟，产卵后要及时取出粘有卵粒的鱼巢另池孵化，以防亲鳅吞食卵粒；
4）孵化：将受精卵转移至微流水孵化箱中进行孵化，孵化箱水体溶氧10 mg/L，pH为7.5，水深0.5米，放卵密度为每升水体600粒，在水温为26℃时约35小时即可孵出鳅苗；在微流水孵化箱中进行孵化可以保证仔鳅的孵化率与成活率可达到90%以上，生产效率极高；
5）育苗：育苗池面积为2亩，池底平整无杂草，水深0.5米，进水口架设90目拦网，放苗前10天以20kg/亩漂白粉进行清池，然后进行浮网和试水操作；将鳅苗以40万尾/亩的密度进行培育，选择强化后的丰年虫作为幼体的开口饵料，丰年虫的强化饵料为裂壶藻15份、小球藻15份、卵磷脂脂质体25份、鱼内脏油25 份、鱼蛋白水解物33份、尿素23份、硝酸铵20份、磷酸钙20份、发酵农家肥80份、活性多肽2份充分混合均匀后粉碎成45微米大小的颗粒，强化喂养丰年虫10小时即可投喂给鳅苗；日投喂量为5kg/亩，幼苗长至5cm时育苗结束即可售卖；活性多肽的氨基酸序列为：SCASRCKSRCRARRCRARRCGRFYFGKRCICKCFRC；经过强化后的丰年虫体内多不饱和脂肪酸的含量较高，适合作为泥鳅的开口饵料，该育苗条件有利于提高泥鳅幼体的免疫能力、应激能力、抗菌抗病能力，提高其成活率与生长发育速度。
实施例3：
一种泥鳅的人工繁殖方法，包括：亲鳅培育池的整理、亲鳅强化、人工催产、孵化、育苗、放养，具体包括以下步骤：
亲鳅培育池的整理：选取靠近水源、无污染、水质优良、排灌方便、环境安静的地方搭建亲鳅培育池，池塘面积为2亩、池深为1.2米、水深0.6米，池底与四周铺上厚度为0.4mm的塑料薄膜，塑料薄膜延伸至池坝外侧，池底铺软腐泥25cm，进排水口都要蒙上80目的筛绢网；用生石灰100kg/亩进行干法消毒，进水15cm，再次施生石灰30kg/亩化水后全池泼洒，加水至0.6米后施用腐熟的农家肥60kg/亩肥水，待池水呈浅绿色时先用几尾泥鳅入池养殖3天测试毒性，无恙后方可大规模培育亲鳅；亲鳅培养期间，控制水温为17℃，pH为7.0，每隔15天可使用20g枯草芽孢杆菌、20g乳酸菌、20g双歧杆菌混合生物制剂向池内泼洒1次，以调节水质；对培育池进行消毒、肥水、试养，有利于亲鳅的强化培养；
亲鳅强化：选取雌泥鳅要腹部膨大，有弹性，将其肚皮向上，颜色微红用手触摸肋骨明显，体重50g，体长20cm；雄泥鳅选取标准为个体较大，体质健壮，活动能力强，体重40g,体长15cm；强化密度为200kg/亩，强化期间以蚯蚓14份、蝇蛆12份、禽畜下脚料25份、鱼粉6份、豆粕35份、麦麸30份、酵母粉5份、维生素C 1份、维生素A 2份、浮萍24份、碎菜叶35份混合均匀作为强化饲料，日投喂量为泥鳅体重的8%，每天投喂2次；取北沙参1份、川贝母2份、白及2份、紫河车2份、巴戟天3份、熟地黄5份、菟丝子3份、淫羊藿1.5份混合后放于水中煎煮，将得到的中草药组合汤药泼洒在亲鳅培育池中；较低的密度和全面的营养条件有利于泥鳅性腺的成熟，中草药组合汤药有利于亲鳅发育成熟，为人工催产做准备；
人工催产：以注射催产素的方法进行人工催产，催产素为比例为2:3：100的促黄体素释放素类似物、绒毛膜促性腺激素与地欧酮，注射之前进行麻醉，注射部位为胸腔，扎针3毫米，注射方向与体轴腹面成45°角，雌鳅注射药液0.2ml、雄鳅0.1ml，注射时可用湿纱布包住亲鳅露出注射部位进行注射；亲鳅注射催产素后，按雌雄比例1∶3放入悬挂在鱼池水中用60目筛绢制成的网箱内，箱中放养水草并用棕片或杨柳根须做成鱼巢，在28℃水温条件下，注射后的亲鳅经10小时便开始发情，表现为亲鳅在水中激烈翻腾，继而雄鳅卷住雌鳅身体产卵、排精，卵产于鱼巢上，产卵过程约30分钟，产卵后要及时取出粘有卵粒的鱼巢另池孵化，以防亲鳅吞食卵粒；先行麻醉后进行胸腔注射催产素可以促进亲鳅排卵排精，胸腔注射不会对亲鳅造成伤害；
孵化：将受精卵转移至微流水孵化箱中进行孵化，孵化箱水体溶氧10 mg/L，pH为7.5，水深0.5米，放卵密度为每升水体600粒，在水温为26℃时约35小时即可孵出鳅苗；在微流水孵化箱中进行孵化可以保证仔鳅的孵化率与成活率可达到90%以上，生产效率极高；
育苗：育苗池面积为2亩，池底平整无杂草，水深0.5米，进水口架设60目拦网，放苗前10天以20kg/亩漂白粉进行清池，然后进行浮网和试水操作；将鳅苗以35万尾/亩的密度进行培育，选择强化后的丰年虫作为幼体的开口饵料，丰年虫的强化饵料为裂壶藻15份、小球藻14份、卵磷脂脂质体22份、鱼内脏油24份、鱼蛋白水解物30份、尿素20份、硝酸铵18份、磷酸钙16份、发酵农家肥80份、活性多肽2份充分混合均匀后粉碎成30微米大小的颗粒，强化喂养丰年虫10小时即可投喂给鳅苗；日投喂量为5kg/亩，幼苗长至5cm时育苗结束；活性多肽的氨基酸序列为：SCASRCKSRCRARRCRARRCGRFYFGKRCICKCFRC；活性多肽可以促进丰年虫对强化饵料的消化吸收，提高强化效率，经过强化后的丰年虫体内多不饱和脂肪酸的含量较高，适合作为泥鳅的开口饵料，该育苗条件有利于提高泥鳅幼体的免疫能力、应激能力、抗菌抗病能力，提高其成活率与生长发育速度；
放养：泥鳅幼苗长至5cm时即可放养，放养密度为3万尾/亩，在泥鳅池中可适当搭养中上层鱼类，如草、鲢、鳙等夏花鱼种，放养时间一般在9月，养殖时间8个月。
本发明的操作步骤中的常规操作为本领域技术人员所熟知，在此不进行赘述。
以上所述的实施例对本发明的技术方案进行了详细说明，应理解的是以上所述仅为本发明的具体实施例，并不用于限制本发明，凡在本发明的原则范围内所做的任何修改、补充或类似方式替代等，均应包含在本发明的保护范围之内。
SEQUENCE LISTING
<110> 兰溪市普润斯水产养殖技术有限公司
<120> 一种泥鳅的人工繁殖方法
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 36
<212> PRT
<213> 人工合成
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Arg
1 5 10 15
Ala Arg Arg Cys Gly Arg Phe Tyr Phe Gly Lys Arg Cys Ile Cys Lys
20 25 30
Cys Phe Arg Cys
35
Claims (10)
1.一种泥鳅的人工繁殖方法，包括：亲鳅培育池的整理、亲鳅强化、人工催产、孵化、育苗、放养，其特征在于，所述的育苗步骤为将鳅苗进行培育，将丰年虫强化喂养8-10小时即可投喂给鳅苗，日投喂量为3-5kg/亩，幼苗长至3-5cm时育苗结束。
2.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的亲鳅培育池的整理步骤为：选取靠近水源、无污染、水质优良、排灌方便、环境安静的地方搭建亲鳅培育池，池塘面积为0.5-3亩、池深为0.8-1.5米、水深0.4-0.8米，池底与四周铺上厚度为0.3-0.5mm的塑料薄膜，塑料薄膜延伸至池坝外侧，池底铺软腐泥20-30cm，进排水口都要蒙上70-90目的筛绢网；用生石灰80-150kg/亩进行干法消毒，进水10-25cm，再次施生石灰20-50kg/亩化水后全池泼洒，加水至0.4-0.8米后施用腐熟的农家肥50-90kg/亩肥水，待池水呈浅绿色时先用几尾泥鳅入池养殖2-3天测试毒性，无恙后方可大规模培育亲鳅；亲鳅培养期间，控制水温为15-17℃，pH为6.5-8.5，每隔10-15 天可使用50-100g益生菌作为微生态制剂向池内泼洒1次，以调节水质。
3.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的亲鳅培育池的整理步骤中的益生菌为光合细菌或枯草芽孢杆菌或纳豆芽孢杆菌或乳酸菌或酵母菌或双歧杆菌的至少3种混合菌群。
4.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的亲鳅强化步骤为：选取雌泥鳅要腹部膨大，有弹性，将其肚皮向上，颜色微红用手触摸肋骨明显，体重40-60g，体长20-25cm；雄泥鳅选取标准为个体较大，体质健壮，活动能力强，体重35-50g,体长15-20cm；强化密度为200-250kg/亩，强化期间以蚯蚓10-15份、蝇蛆10-15份、禽畜下脚料20-30份、鱼粉5-10份、豆粕30-40份、麦麸30-40份、酵母粉5-10份、维生素C1-2份、维生素A1-2份、浮萍20-25份、碎菜叶30-40份混合均匀作为强化饲料，日投喂量为泥鳅体重的6-10%，每天投喂2-3次；取北沙参1-2份、川贝母1-2份、白及2-3份、紫河车1-2份、巴戟天2-3份、熟地黄3-5份、菟丝子2-3份、淫羊藿1-1.5份混合后放于水中煎煮，将得到的中草药组合汤药泼洒在亲鳅培育池中。
5.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的人工催产步骤为：以注射催产素的方法进行人工催产，注射之前采用MS-222进行麻醉，注射部位为胸腔，扎针2-3毫米，注射方向与体轴腹面成30-45°角，雌鳅注射药液0.15-0.2ml、雄鳅0.08-0.1ml，注射时可用湿纱布包住亲鳅露出注射部位进行注射；亲鳅注射催产素后，按雌雄比例1∶2-3放入悬挂在鱼池水中用40-60目筛绢制成的网箱内，箱中放养水草并用棕片或杨柳根须做成鱼巢，在25-28℃水温条件下，注射后的亲鳅经8-10小时便开始发情，产卵过程20-30分钟。
6.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的人工催产步骤中的催产素为：比例为2-3:2-3：100的促黄体素释放素类似物与绒毛膜促性腺激素与地欧酮。
7.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的孵化步骤为：将受精卵转移至微流水孵化箱中进行孵化，孵化箱水体溶氧6-10 mg/L，pH为7.0-7.5，水深0.4-0.5米，放卵密度为每升水体400-600粒，在水温为24-26℃时约30-35小时即可孵出鳅苗。
8.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的育苗步骤为：育苗池面积为1-2亩，池底平整无杂草，水深0.3-0.5米，进水口架设60-90目拦网，放苗前7-10天以15-20kg/亩漂白粉进行清池，然后进行浮网和试水操作；将鳅苗以30-40万尾/亩的密度进行培育，选择强化后的丰年虫作为幼体的开口饵料，丰年虫的强化饵料为裂壶藻10-15份、小球藻10-15份、卵磷脂脂质体20-25份、鱼内脏油20-25 份、鱼蛋白水解物28-33份、尿素20-23份、硝酸铵18-20份、磷酸钙15-20份、发酵农家肥60-80份、活性多肽1-2份充分混合均匀后粉碎成10-45微米大小的颗粒，强化喂养丰年虫8-10小时即可投喂给鳅苗；日投喂量为3-5kg/亩，幼苗长至3-5cm时育苗结束。
9.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的育苗步骤中的活性多肽的氨基酸序列为：SCASRCKSRCRARRCRARRCGRFYFGKRCICKCFRC。
10.根据权利要求1所述的一种泥鳅的人工繁殖方法，其特征在于：所述的放养步骤为：泥鳅幼苗长至3-5cm时即可售卖或者放养，放养密度为2-3.5万尾/亩，在泥鳅池中可适当搭养中上层鱼类，如草、鲢、鳙等夏花鱼种，放养时间在9-10月，养殖时间8-9个月。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710642431.1A CN107494343A (zh) | 2017-07-31 | 2017-07-31 | 一种泥鳅的人工繁殖方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710642431.1A CN107494343A (zh) | 2017-07-31 | 2017-07-31 | 一种泥鳅的人工繁殖方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107494343A true CN107494343A (zh) | 2017-12-22 |
Family
ID=60689350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710642431.1A Pending CN107494343A (zh) | 2017-07-31 | 2017-07-31 | 一种泥鳅的人工繁殖方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107494343A (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220912A (zh) * | 2018-09-28 | 2019-01-18 | 广东工业大学 | 一种斑马鱼保种培养方法 |
| CN109349172A (zh) * | 2018-12-04 | 2019-02-19 | 江西省农业科学院畜牧兽医研究所 | 一种泥鳅与大鳞副泥鳅杂交繁育方法 |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03123449A (ja) * | 1989-10-06 | 1991-05-27 | Nippon Suisan Kaisha Ltd | 仔魚用餌料生物と仔魚の増養殖方法 |
| JPH05316958A (ja) * | 1992-05-19 | 1993-12-03 | Riken Vitamin Co Ltd | 仔稚魚用生物餌料と仔稚魚の増養殖方法 |
| CN1653938A (zh) * | 2005-03-09 | 2005-08-17 | 青岛森淼实业有限公司 | 微藻饵料营养强化剂配方及其制法 |
| CN101810246A (zh) * | 2009-11-13 | 2010-08-25 | 厦门汇盛生物有限公司 | 一种复合营养强化剂及其制备方法 |
| CN104206359A (zh) * | 2014-08-20 | 2014-12-17 | 天津海友佳音生物科技股份有限公司 | 一种利用微藻和其他微生物复合饵料养殖卤虫的方法 |
| CN105766810A (zh) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | 一种卤虫的室内低成本、高密度、可持续养殖方法 |
| CN106665414A (zh) * | 2015-11-05 | 2017-05-17 | 尤世元 | 一种泥鳅养殖技术 |
| CN106841519A (zh) * | 2016-12-30 | 2017-06-13 | 浙江海洋大学 | 一种虎斑乌贼开口饵料营养强化剂的筛选方法及其应用 |
-
2017
- 2017-07-31 CN CN201710642431.1A patent/CN107494343A/zh active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03123449A (ja) * | 1989-10-06 | 1991-05-27 | Nippon Suisan Kaisha Ltd | 仔魚用餌料生物と仔魚の増養殖方法 |
| JPH05316958A (ja) * | 1992-05-19 | 1993-12-03 | Riken Vitamin Co Ltd | 仔稚魚用生物餌料と仔稚魚の増養殖方法 |
| CN1653938A (zh) * | 2005-03-09 | 2005-08-17 | 青岛森淼实业有限公司 | 微藻饵料营养强化剂配方及其制法 |
| CN101810246A (zh) * | 2009-11-13 | 2010-08-25 | 厦门汇盛生物有限公司 | 一种复合营养强化剂及其制备方法 |
| CN104206359A (zh) * | 2014-08-20 | 2014-12-17 | 天津海友佳音生物科技股份有限公司 | 一种利用微藻和其他微生物复合饵料养殖卤虫的方法 |
| CN106665414A (zh) * | 2015-11-05 | 2017-05-17 | 尤世元 | 一种泥鳅养殖技术 |
| CN105766810A (zh) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | 一种卤虫的室内低成本、高密度、可持续养殖方法 |
| CN106841519A (zh) * | 2016-12-30 | 2017-06-13 | 浙江海洋大学 | 一种虎斑乌贼开口饵料营养强化剂的筛选方法及其应用 |
Non-Patent Citations (5)
| Title |
|---|
| 农业部人事劳动司 等: "《全国农业职业技能培训教材 海水水生动物苗种繁育技术（下册）》", 30 September 2014, 中国农业出版社 * |
| 张海龙 等: "泥鳅规模化繁育技术", 《水产养殖》 * |
| 杨培歌: "泥鳅高效人工繁殖技术", 《河南水产》 * |
| 王冬武 等: "《特种水产生态养殖 泥鳅生态养殖》", 31 October 2013, 湖南科学技术出版社 * |
| 陆专灵 等: "开口饵料对泥鳅苗种存活率的影响及其生长曲线拟合分析", 《南方农业学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220912A (zh) * | 2018-09-28 | 2019-01-18 | 广东工业大学 | 一种斑马鱼保种培养方法 |
| CN109349172A (zh) * | 2018-12-04 | 2019-02-19 | 江西省农业科学院畜牧兽医研究所 | 一种泥鳅与大鳞副泥鳅杂交繁育方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104857337B (zh) | 一种草亲鱼用发酵中药微生物制剂及其制备方法 | |
| CN104430089B (zh) | 一种无公害淡水龙虾的养殖方法 | |
| AU2020103683A4 (en) | Method for breeding macrobrachium nipponense seed in saline-alkali water | |
| CN106069953A (zh) | 一种优质高产清水草鱼的养殖方法 | |
| CN104705229B (zh) | 一种瓣结鱼与河蟹的生态混养方法 | |
| CN105706968A (zh) | 一种草鱼生态养殖方法 | |
| CN101897309A (zh) | 黄鳝、泥鳅的生态养殖方法 | |
| CN103181343A (zh) | 一种大型鱼塘种、养殖一体化的生态养殖方法 | |
| CN104381213A (zh) | 青蛙的高密度养殖方法 | |
| CN104412915A (zh) | 一种青虾从幼虾期到成虾期的健康养殖技术 | |
| CN107836304A (zh) | 稻田中养殖小龙虾的方法 | |
| CN104920298B (zh) | 种蛙温室管理方法 | |
| CN105284675B (zh) | 一种用于养殖泥鳅的生物饵料制备方法 | |
| CN105210938B (zh) | 一种泥鳅微生物养殖法 | |
| CN105230556A (zh) | 中华鳖全程无公害生态养殖方法 | |
| CN104396872B (zh) | 一种豪猪标准化养殖方法 | |
| CN107455291A (zh) | 一种黄颡鱼的池塘养殖方法 | |
| CN105941824A (zh) | 中药微生物益生菌原液、饲料、肥料和农药及制备方法 | |
| CN103392641B (zh) | 一种精细化曼氏无针乌贼池塘养殖方法 | |
| CN106852299A (zh) | 黄缘闭壳龟人工养殖方法 | |
| CN106035146A (zh) | 青鱼、鲫鱼池塘套养技术 | |
| CN107494344A (zh) | 一种黄颡鱼鱼苗的人工培育方法 | |
| CN106974134A (zh) | 提高杂交鳢苗种成活率的饲料添加剂及其饲料和制备方法 | |
| CN107242161A (zh) | 一种黑鲷鱼苗种培育技术 | |
| CN107494343A (zh) | 一种泥鳅的人工繁殖方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171222 |
|
| RJ01 | Rejection of invention patent application after publication |