CN107487859A - A kind of method of fish-farming with sewage microalgae production livestock fodder additives - Google Patents

A kind of method of fish-farming with sewage microalgae production livestock fodder additives Download PDF

Info

Publication number
CN107487859A
CN107487859A CN201710938494.1A CN201710938494A CN107487859A CN 107487859 A CN107487859 A CN 107487859A CN 201710938494 A CN201710938494 A CN 201710938494A CN 107487859 A CN107487859 A CN 107487859A
Authority
CN
China
Prior art keywords
sewage
microalgae
adsorption column
hollow
parts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710938494.1A
Other languages
Chinese (zh)
Other versions
CN107487859B (en
Inventor
覃央央
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Edfu Biotechnology Co., Ltd.
Original Assignee
Guangxi Nanning Rong Weide Amperex Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Nanning Rong Weide Amperex Technology Ltd filed Critical Guangxi Nanning Rong Weide Amperex Technology Ltd
Priority to CN201710938494.1A priority Critical patent/CN107487859B/en
Publication of CN107487859A publication Critical patent/CN107487859A/en
Application granted granted Critical
Publication of CN107487859B publication Critical patent/CN107487859B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/30—Wastewater or sewage treatment systems using renewable energies
    • Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Animal Husbandry (AREA)
  • Birds (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
  • Hydrology & Water Resources (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Physiology (AREA)
  • Dispersion Chemistry (AREA)
  • Botany (AREA)

Abstract

The present invention provides a kind of method of fish-farming with sewage microalgae production livestock fodder additives, by isolating crude protein product and high protein product from algae, so that the protein content in poultry feed is higher than conventional feed, enough protein is provided for home poultry raising, the protein derives from algae simultaneously, thus can reduce corn and dregs of beans usage amount.The present invention also describes a kind of method of sewage culture microalgae, using modification of chitosan, modified montmorillonite used it be prepared into the hollow adsorption column that adsorbed film assembles the metal ion in sewage is adsorbed, sewage is set to pass through adsorption column by pump suction function, sewage stops in adsorption column, contacted with immobilized microalgae, microalgae carries out growth and breeding using the organic nutrient substance in sewage.Carry out sewage disposal using this method, can effectively remove metal ion, carbon, nitrogen, the material such as phosphorus in decontaminated water, at the same can large-scale culturing micro-algae, reach sewage disposal and microdisk electrode dual purpose.

Description

A kind of method of fish-farming with sewage microalgae production livestock fodder additives
Technical field
The invention belongs to Feed Production Technology and technical field of sewage, is more particularly to produced using fish-farming with sewage microalgae The method of livestock fodder additives.
Background technology
Poultry feed one is to wheat, paddy or rice bran, wheat bran.General feeds form：Energy feed, rice bran, beanstalk chaff, Bavin chaff and wheat bran etc., account for 50 ~ 60%；Vegetable protein feed, soya-bean cake, rape cake and cotton benevolence cake, accounts for 10 ~ 20%；Poultry property Protein feeds primary raw material comes from and cereal crops, is unfavorable for the long-term needs of human survival.
Microalgae is that have the protophyte of photosynthetic ability, and micro-algae propagation survival ability is extremely strong, can be from waste water Various inorganic elements and organic matter are absorbed, microalgae contains multivitamin and protein and polysaccharide material, in the prior art, micro- Algae cultivation is in laboratory stage, and natural microalgae quantity is few, is not suitable for the production of extensive accessory substance.
With China's economic development, livestock and poultry cultivation sewage, municipal wastewater and trade effluent increasingly increase as main pollution Source, these discharges of sewage are big, and COD, carbon, the content of nitrogen and phosphorous are high, simultaneously containing various heavy metal substances.Traditional aerobic activity is dirty Mud treatment technology input cost is high, and operation energy consumption is big, Treatment of Sludge trouble, and dirt can not be fully utilized in the microorganism in activated sludge In the nutriment such as the material in water, most of carbon, nitrogen, phosphorus also residual water.Supported using sewage as cheap culture medium scale It is study hotspot both domestic and external to grow processing sewage.
A kind of methods of waste water microalgae processing feed of application for a patent for invention CN201510390515.1, this method is will be useless After Aquaponic, collection, the microalgae of precipitation and maize peel, bacillus subtilis mix, aerobic fermentation is accumulated on the ground, utilizes hair Caused high temperature fully kills germ during ferment；The microalgae material of aerobic fermentation mixes with live pig perfect compound feed again after cooling Close, Lactobacillus plantarum and long handle trichoderma are added in mixed process, regulation moisture to 40-50%, carries out anaerobic fermentation to pH Feeding live pig is taken out during 3.5-5.0.The present invention improves the utilization rate of waste water, reduces the drying expense of microalgae, kills waste water In pathogen, improve the biological safety that microalgae utilizes, promote microalgae fodder utilize.Waste water microalgae processing The beneficial bacterial content rise of feed, the production performance of live pig improve.Number of patent application CN201210448432.X, a kind of poultry are raised Material and preparation method thereof, by isolating crude protein product and high protein product from algae so that the albumen in poultry feed Content is higher than conventional feed, provides enough protein, while the source of the protein and algae for home poultry raising, thus can be with Reduce corn and dregs of beans usage amount；In addition to producing poultry feed, it can enter in the polysaccharide solution after its by-product pint albumen Row decolouring obtains refined polysaccharide.Number of patent application CN201510996151.1 microalgaes feed addictive and feed, improve eggs egg The content of omega-3 polyunsaturated fatty acids and beta carotene in Huang, improves the immunity of organisms of birds, promotes to nutriment Digest and assimilate, effectively improve the egg laying performance of egg birds.
In the prior art, it is few also in laboratory stage, Chinese style stage and industrialization large-scale culture using microdisk electrode Have been reported that, which kind of material culture microalgae to be also the focus studied at this stage using；Studied at present using microalgae processing sewage Focus, microalgae utilize the organic substance amount reproduction in sewage, reach sewage disposal purpose, but the collection of microalgae turns into new again The problem of, meanwhile, it is also less at present for the report of the research on utilization of microalgae idiotrophic value.
The content of the invention
To make up the vacancy of prior art, fill up and study few lack using fish-farming with sewage microalgae production livestock fodder additives Fall into, the present invention provides a kind of method of fish-farming with sewage microalgae production livestock fodder additives.
Sewage after being handled using the present invention, BOD ＜ 110mg/L, COD ＜ 380mg/L, suspension ＜ 20mg/L, ammonia nitrogen ＜ 40mg/L, total phosphorus ＜ 4mg/L.Sewage after processing can be used for irrigating, cultivating, cleaning；Turn out the microalgae heavy metal come Content is low, and protein, polysaccharide material and its vitamin substances content reach the standard of feed addictive, is feed addictive city Field provides technical support.
The present invention is by being achieved in that：
（1）Band stomata snake pipe is installed at sewage reaction tank bottom, down, coiled pipe is connected stomata with ozone generating-device；
（2）The preparation and installation of adsorption column：
A. 50 parts of chitosans are weighed, is added in 60ml 95% ethanol solution and soaks, 60 DEG C of swelling 2h of constant temperature；（2）By 150 parts Vanillic aldehyde is dissolved in 400ml 95% ethanol, and the soak for pouring into chitosan obtains mixed liquor；（3）Mixed liquor is positioned over micro- In ripple quick reaction device, 70 DEG C of heating-up temperature is set, reacts 10min, microwave power 300w, stirring；（4）Reaction is completed, cold But filter afterwards, ethanol is washed, and washing, is dried under vacuum to constant weight, is obtained modification of chitosan powder；
B. 20 parts of natural smectite is weighed, 5% sodium hydroxide of natural smectite quality is weighed, by solid-liquid mass ratio 1:10 add Enter 200ml distilled water, regulation pH is 5.6,60 DEG C of stirring reaction 1h in water bath with thermostatic control, the freeze-day with constant temperature at 80 DEG C, fine grinding 100 mesh sieve, it is modified montmorillonite used to obtain sodium；
C. it is 10 to take cell concentration5/ ml microalgae solution, aseptically, with sodium alginate soln, modification of chitosan 1:1: 1 well mixed obtains mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out 20 ~ 30min of solidification, obtains marine alga Sour calcium coagulates pearl；Calcium alginate is coagulated into pearl and uses distillation water washing 2 ~ 3 times, you being fixed microalgae glueballs；
The sodium alginate concentration is 20 ~ 25mg/ml；Described calcium chloride solution concentration is 18 ~ 22mg/ml；
The mixed liquor is 1 with calcium chloride solution volume ratio：（1.5~2）；
D. 40 ~ 80 parts of Kynoar powder is taken, is dissolved in 25 ~ 50 parts of dibutyl phthalates, adds 50 ~ 100 parts of modifications Chitosan powder, 50 ~ 100 parts of modified montmorillonite used powder, 2 ~ 10 parts of Geniposides, under nitrogen protection in 210 ~ 223 DEG C of oil baths After 4 ~ 6h of stirring is mixed into uniform solution, standing and defoaming obtains polymer；Polymer is fed by the Weightlessness balance of 5 ‰ measuring accuracies Material machine feeds double screw extruder, under 250 ~ 300rpm and 2 ~ 5MPa mixing kneading effect, material is sufficiently mixed uniformly Into the casting solution of stable homogeneous, casting solution enters shower nozzle by spinning pump with 90 ~ 100g/min speed accurate measurement, interior Chamber is using the nitrogen of 36 ~ 50ml/min flow velocitys as hollow supporting body, into 298K's after the air section evaporation of certain altitude Occur to be separated and solidify in water-bath, collected thick hollow-fibre membrane by collecting wheel with 25m/min speed；
E. thick hollow-fibre membrane d being prepared is immersed in 95% industrial alcohol 24 hours, and extraction dibutyl phthalate is dilute Agent is released, during which need to change ethanol 2 times, it is ensured that diluent extraction is clean, and it is dry with clear water rinsing will to extract the hollow-fibre membrane finished It is net to obtain hollow-fibre membrane.
F. hollow-fibre membrane is taken, is assembled into duplicature post, the wide spaces of 3 ~ 7cm, outermost tunic bottom are left among duplicature Portion is provided with dump valve, obtains adsorption column；
G. multiple adsorption columns are vertically fixed with brandreth, hollow space parallel connection in innermost layer is connected with suction pump, is positioned over reaction tank In；
（3）Immobilized microalgae cell is filled in the intermediate space of adsorption column duplicature, seals adsorption column；
（4）Open sewage pump and sewage is injected into reaction tank, when sewage is not to adsorption column 50 ~ 70%, stop pumping；Open ozone Generating means, ozone aeration quantity are 15 ~ 40g/h, and action time is 10 ~ 20min/h, after ozonization 5h, open suction pump, Regulation flow is 1 ~ 1.4L/min, 15 ~ 30min of working time, 40 ~ 60min of time of having a rest, sewage is entered adsorption column and fixation After change microalgae is reacted, reacted sewage is discharged into water purifying tank；
（5）After operation 4 ~ 7 days, stopping is filled with ozone, stops sewage pump, stopped pumping pump, and immobilized microalgae is passed through into adsorption column Bottom-discharge valve is discharged, and is collected；Hollow-fibre membrane is cleaned 2 hours with 2 ~ 5wt%HCl, then with 2000 ~ 3000mg/L NaClO+ 1000 ~ 2000mg/L NaOH are cleaned 2 hours, and every component cleaning flux be 15 ~ 30L/h, 23 ~ 25 DEG C of temperature, eluent collection It is used to metal ion afterwards reclaim.
The conservation rate that the cleaning can improve tensile strength is 80% ~ 82.9%.
（6）Collect immobilized microalgae and carry out broken wall treatment, by microalgae drying and crushed using heated-air drying, take microalgae powder 100 ~ 200 parts, the cellulase and enzyme activity that 10 ~ 20 parts of enzyme activities of addition are 1400 ~ 1800U/g are 60000 ~ 70000U/g's Neutral proteinase 1:1 combination, after mixing 4 ~ 6h of reaction, centrifuged 10 ~ 20 minutes under 5000 ~ 6000rpm, obtain supernatant I and sink Shallow lake I；
（7）Supernatant I is added in 4 DEG C of isometric cold ethanol mix 12 ~ 14h after, filtering and concentrating to original volume 1/5 ~ 1/4, then it is freeze-dried, obtains microalgae powder i.e. livestock fodder additives；
（8）Microalgae powder is added in poultry feed, addition is 10% ~ 15%, feeding poultry.
As a further improvement on the present invention, the preparation method of the modification of chitosan is as follows：
（1）50 parts of chitosans are weighed, is added in 60ml 95% ethanol solution and soaks, 60 DEG C of swelling 2h of constant temperature；（2）By 150 Part vanillic aldehyde is dissolved in 400ml 95% ethanol, and the soak for pouring into chitosan obtains mixed liquor；（3）Mixed liquor is positioned over In microwave quick reaction device, 70 DEG C of heating-up temperature is set, reacts 10min, microwave power 300w, stirring；（4）Reaction is completed, Cooled and filtered, ethanol are washed, and washing, are dried under vacuum to constant weight, are obtained modification of chitosan powder.
The chitosan of vanillic aldehyde graft modification, there is more preferable filming performance, while Cu can be removed very well2+、Zn2+, to having Machine thing COD adsorption rate can reach 25mg/g；
As a further improvement on the present invention, the modified montmorillonite used preparation method is as follows：
20 parts of natural smectite is weighed, 5% sodium hydroxide of natural smectite quality is weighed, by solid-liquid mass ratio 1:10 add 200ml distilled water, regulation pH are 5.6,60 DEG C of stirring reaction 1h in water bath with thermostatic control, the freeze-day with constant temperature at 80 DEG C, and fine grinding crosses 100 Mesh sieve, it is modified montmorillonite used to obtain sodium.
The raw material of specific proportioning carries out the property montmorillonite that sodium changes, to Cu2+、Pb2+、Zn2+、Cd2+Adsorption rate it is reachable respectively 99.2%th, 99.8%, 99.5% and 99.3%.
As a further improvement on the present invention, the immobilized microalgae preparation method is as follows：
It is 10 to take cell concentration3/ ml microalgae solution, aseptically, with sodium alginate soln, modification of chitosan 1:1:1 It is well mixed to obtain mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out 20 ~ 30min of solidification, obtains marine alga Sour calcium coagulates pearl；Calcium alginate is coagulated into pearl and uses distillation water washing 2 ~ 3 times, you being fixed microalgae glueballs.The sodium alginate Concentration is 20 ~ 25mg/ml；Described calcium chloride solution concentration is 18 ~ 22mg/ml；The mixed liquor and calcium chloride solution volume Than for 1：（1.5~2）.
As a further improvement on the present invention, described Kynoar ethylene molecule amount is 30KDa ~ 40Kda, gathers inclined fluorine Ethylene concentration is 30 ~ 35wt%.
Polymer molecular weight size influences Kynoar system viscosity and membrane aperture size, and then influences pure water and pass through Rate.When polymer molecular weight increases, system viscosity increases, and the growth of stingy drop and nucleus is limited, when polymer concentrated phase solidifies Corresponding drop and spherulite size are smaller, and corresponding membrane aperture is smaller.
As a further improvement on the present invention, dibutyl phthalate, modification of chitosan, modified montmorillonite used powder weight Score ratio is 1:2:2~2:2:5.
The Kynoar ethene that molecular weight is 30KDa ~ 40Kda, concentration is 30 ~ 35wt%, with the fourth of phthalic acid two Ester, modification of chitosan, modified montmorillonite used powder ratio of weight and number are 1:2:2~2:2:5, the performance of the film prepared is：Pure water 580 ~ 800Lm of flux-2h-1（298K）, 0.101 ~ 0.115 μm, 15.2 ~ 20.4N of tensile strength of average pore size, porosity 70 ~ 80%。
As a further improvement on the present invention, adsorption column hollow space is not communicated with two membranes intermediate space part.
As a further improvement on the present invention, immobilized microalgae loading 50 ~ 70% in the adsorption column.
As a further improvement on the present invention, the ozonization time is 5h/ days.
Then the present invention is mixed and made into adsorption column film by being modified to chitosan and montmorillonite with Kynoar, Using the adsorption column that is assembled into of immobilized microalgae filling duplicature, sewage passes through the suction-operated of adsorption column outer membrane, and 98% or so Heavy metal ion be adsorbed, the sewage after absorption is entered inside adsorption column to be reacted with immobilized microalgae, and microalgae can be sharp Growth and breeding is carried out with the Organic Carbon And Total Nitrogen phosphorus substance in sewage, further except the carbon nitrogen phosphorus substance in decontaminated water.After microalgae processing Sewage again pass by adsorption column inner layer film after discharge, almost without the polluter such as heavy metal, carbon, nitrogen, phosphorus.
Advantages of the present invention：
1. using the sewage culture microalgae after adsorption column absorption heavy metal ion, richness of the heavy metal ion in microalgae is reduced Collection, microalgae production livestock fodder additives are recycled, the micro algae growth amount cultivated by the present invention is big, is easy to collect, toxic It is low, composite green environmental protection, the requirement of sustainable development.
2. the present invention by being modified to chitosan and montmorillonite is used as membrane material, can adsorb well heavy metal from Son, it can effectively adsorb Pb In Exhausted Water, every, the heavy metal ion such as nickel, molybdenum, while montmorillonite also has and remove malicious function, further only Change sewage.
3. the present invention carries out both culturing microalgae using sewage, sewage can be purified, and can large-scale culturing micro-algae is used In feed additive production, the sustainable development requirement of resource reutilization is reached.
4. adsorption column prepared by the present invention is hybrid organic-inorganic film, the advantages of having concentrated polyvinylidene fluoride separation membrane with And the absorption property of chitosan, montmorillonite, film stable physical property, pure water flux and hydrophily are improved to some extent.
5. the present invention adsorption column there is good pressure-resistant performance, arbitrary size can be made without supporter, membrane module and shape, The advantages that loading density is big in membrane module, unit volume membrane area and flux is big.
Brief description of the drawings
Fig. 1 is the schematic diagram of adsorption column of the present invention.Wherein 1 is outer layer hollow membrane, and 2 be nexine hollow membrane, and 3 be filling microalgae Middle layer segment, 4 be hollow space.
Embodiment
The following examples can make those skilled in the art that the present invention be more fully understood, but not limit in any way The present invention.
Embodiment 1
The present embodiment is for Pig farm wastewater processing.
The preparation of adsorption column and installation method are as follows in the present embodiment：
A. 40Kda, 30wt% 80 parts of Kynoar powder are taken, is dissolved in 25 parts of dibutyl phthalates, 50 parts is added and changes Property Chitosan powder, 50 parts of modified montmorillonite used powder, 2 parts of Geniposides, under nitrogen protection in 210 DEG C of oil baths stir 4h mixing Into after uniform solution, standing and defoaming obtains polymer；Polymer is fed into double spiral shells by the Weightlessness balance feeding machine of 5 ‰ measuring accuracies Bar extruder, under 250rpm and 2MPa mixing kneading effect, material is set to be sufficiently mixed uniformly into the casting solution of stable homogeneous, Casting solution enters shower nozzle by spinning pump with 90g/min speed accurate measurement, and inner chamber is with the nitrogen of 36ml/min flow velocitys As hollow supporting body, it is separated and solidifies into generation in 25 DEG C of water-bath after the air section evaporation of certain altitude, with 25m/min speed is collected thick hollow-fibre membrane by collecting wheel；
B. thick hollow-fibre membrane d being prepared is immersed in 95% industrial alcohol 24 hours, and extraction dibutyl phthalate is dilute Agent is released, during which need to change ethanol 2 times, it is ensured that diluent extraction is clean, and it is dry with clear water rinsing will to extract the hollow-fibre membrane finished It is net to obtain hollow-fibre membrane.
C. hollow-fibre membrane is taken, is assembled into duplicature post, the wide spaces of 7cm, outermost tunic bottom are left among duplicature Dump valve is provided with, obtains adsorption column；
D. multiple adsorption columns are vertically fixed with brandreth, hollow space parallel connection in innermost layer is connected with suction pump, is positioned over reaction tank In；
The present embodiment concrete operation step is as follows：
Immobilized microalgae is filled into adsorption column, seals adsorption column, opens sewage pump by after the processing of pig farm one-level grid Sewage injects reaction tank, when sewage is not to adsorption column 70%, stops pumping；Ozone generating-device is opened, ozone aeration quantity is 40g/h, action time 20min/h, after ozonization 5h, suction pump is opened, regulation flow is 1.4L/min, the working time 30min, time of having a rest 60min, after sewage is reacted into adsorption column with immobilized microalgae, reacted sewage is discharged into Water purifying tank；After operation 7 days, stopping is filled with ozone, stops sewage pump, stopped pumping pump, and immobilized microalgae is passed through into adsorption column bottom Portion's dump valve discharge, is collected；Hollow-fibre membrane is cleaned 2 hours with 2wt%HCl, then with 2000mg/L NaClO+1000mg/L NaOH is cleaned 2 hours, 23 DEG C of temperature.The conservation rate that the cleaning can improve tensile strength is 80%；
Collect immobilized microalgae and carry out broken wall treatment, by microalgae drying and crushed using heated-air drying, take 100 parts of microalgae powder, add Enter the cellulase that 10 parts of enzyme activities are 1400U/g and the neutral proteinase 1 that enzyme activity is 60000U/g:1 combination, mixes reaction After 4h, centrifuged 10 minutes under 5000rpm, obtain supernatant I and precipitation I；
Supernatant I is added to after mixing 12h in 4 DEG C of isometric cold ethanol, the 1/5, Ran Houjin of filtering and concentrating to original volume Row freeze-drying, obtains microalgae powder i.e. livestock fodder additives；
Microalgae powder is added in poultry feed, addition 10%, feeding poultry.
In the present embodiment, immobilized microalgae loading 70% in adsorption column.Microalgae concentration after culture is 4.2g/L.
Embodiment 2
The present embodiment is used for sanitary sewage disposal.
Adsorption column is prepared as follows with installation method in the present embodiment：
A. 40 parts of 30Kda, 35wt% Kynoar powder is taken, is dissolved in 30 parts of dibutyl phthalates, adds 30 parts of modifications Chitosan powder, 75 parts of modified montmorillonite used powder, 5 parts of Geniposides, 5h is stirred in 215 DEG C of oil baths under nitrogen protection and is mixed into After uniform solution, standing and defoaming obtains polymer；Polymer is fed into twin-screw by the Weightlessness balance feeding machine of 5 ‰ measuring accuracies Extruder, under 280rpm and 4MPa mixing kneading effect, material is set to be sufficiently mixed uniformly into the casting solution of stable homogeneous, casting Film liquid enters shower nozzle by spinning pump with 100g/min speed accurate measurement, and inner chamber is made with the nitrogen of 45ml/min flow velocitys For hollow supporting body, it is separated and solidifies into generation in 298K water-bath after the air section evaporation of certain altitude, with 25m/min speed is collected thick hollow-fibre membrane by collecting wheel；
B. thick hollow-fibre membrane d being prepared is immersed in 95% industrial alcohol 24 hours, and extraction dibutyl phthalate is dilute Agent is released, during which need to change ethanol 2 times, it is ensured that diluent extraction is clean, and it is dry with clear water rinsing will to extract the hollow-fibre membrane finished It is net to obtain hollow-fibre membrane.
C. hollow-fibre membrane is taken, is assembled into duplicature post, the wide spaces of 3cm, outermost tunic bottom are left among duplicature Dump valve is provided with, obtains adsorption column；
D. multiple adsorption columns are vertically fixed with brandreth, hollow space parallel connection in innermost layer is connected with suction pump, is positioned over reaction tank In；
The present embodiment concrete operation step is as follows：
Open sewage pump and sanitary sewage is injected into reaction tank, when sewage is not to adsorption column 50%, stop pumping；Open ozone hair Generating apparatus, ozone aeration quantity is 15g/h, action time 10min/h, after ozonization 5h, opens suction pump, adjusts flow For 1L/min, working time 15min, time of having a rest 40min, sewage is set to enter after adsorption column reacted with immobilized microalgae, Reacted sewage is discharged into water purifying tank；
After operation 4 days, stopping is filled with ozone, stops sewage pump, and stop pumping pump, and immobilized microalgae is arranged by adsorbing column bottom Go out valve discharge, collect, hollow-fibre membrane is cleaned 2 hours with 4wt%HCl, then with 2500mg/L NaClO+1500mg/L NaOH Cleaning 2 hours, every component cleaning flux are 22L/h, 24 DEG C of temperature.The conservation rate that the cleaning can improve tensile strength is 81.6%；
Collect immobilized microalgae and carry out broken wall treatment, by microalgae drying and crushed using heated-air drying, take 200 parts of microalgae powder, add Enter the cellulase that 20 parts of enzyme activities are 1800U/g and the neutral proteinase 1 that enzyme activity is 70000U/g:1 combination, mixes reaction After 6h, centrifuged 20 minutes under 6000rpm, obtain supernatant I and precipitation I；
Supernatant I is added to after mixing 14h in 4 DEG C of isometric cold ethanol, the 1/4, Ran Houjin of filtering and concentrating to original volume Row freeze-drying, obtains microalgae powder i.e. livestock fodder additives；
Microalgae powder is added in poultry feed, addition 15%, feeding poultry.
In the present embodiment, immobilized microalgae loading 50% in adsorption column, the microalgae density after culture is 5.0g/L.
Embodiment 3
The present embodiment is used for municipal sewage treatment.
Adsorption column is prepared as follows with installation method in the present embodiment：
A. 80 parts of 35Kda, 30wt% Kynoar powder is taken, is dissolved in 50 parts of dibutyl phthalates, 100 parts is added and changes Property Chitosan powder, 100 parts of modified montmorillonite used powder, 10 parts of Geniposides, under nitrogen protection in 223 DEG C of oil baths stir 6h mix After synthesizing uniform solution, standing and defoaming obtains polymer；Polymer is double by the Weightlessness balance feeding machine feeding of 5 ‰ measuring accuracies Screw extruder, under 300rpm and 5MPa mixing kneading effect, material is set to be sufficiently mixed uniformly into the casting film of stable homogeneous Liquid, casting solution enter shower nozzle by spinning pump with 100g/min speed accurate measurement, and inner chamber is with 50ml/min flow velocitys Nitrogen is as hollow supporting body, into generation phase separation in 298K water-bath and admittedly after the air section evaporation of certain altitude Change, collected thick hollow-fibre membrane by collecting wheel with 25m/min speed；
B. thick hollow-fibre membrane d being prepared is immersed in 95% industrial alcohol 24 hours, and extraction dibutyl phthalate is dilute Agent is released, during which need to change ethanol 2 times, it is ensured that diluent extraction is clean, and it is dry with clear water rinsing will to extract the hollow-fibre membrane finished It is net to obtain hollow-fibre membrane.
C. hollow-fibre membrane is taken, is assembled into duplicature post, the wide spaces of 5cm, outermost tunic bottom are left among duplicature Dump valve is provided with, obtains adsorption column；
D. multiple adsorption columns are vertically fixed with brandreth, hollow space parallel connection in innermost layer is connected with suction pump, is positioned over reaction tank In；
The present embodiment concrete operation step is as follows：
Open sewage pump and sanitary sewage is injected into reaction tank, when sewage is not to adsorption column 60%, stop pumping；Open ozone hair Generating apparatus, ozone aeration quantity is 25g/h, action time 20min/h, after ozonization 5h, opens suction pump, adjusts flow For 1.2L/min, working time 20min, time of having a rest 50min, sewage is set to be reacted into adsorption column with immobilized microalgae Afterwards, reacted sewage is discharged into water purifying tank；
After operation 6 days, stopping is filled with ozone, stops sewage pump, and stop pumping pump, and immobilized microalgae is arranged by adsorbing column bottom Go out valve discharge, collect；Hollow-fibre membrane is cleaned 2 hours with 5wt%HCl, then with 3000mg/L NaClO+2000mg/L NaOH Cleaning 2 hours, every component cleaning flux are 30L/h, 25 DEG C of temperature.The conservation rate that the cleaning can improve tensile strength is 82.9%；
（6）Collect immobilized microalgae and carry out broken wall treatment, by microalgae drying and crushed using heated-air drying, take 150 parts of microalgae powder, Add the cellulase that 15 parts of enzyme activities are 1600U/g and the neutral proteinase 1 that enzyme activity is 65000U/g:1 combination, mix anti- After answering 5h, centrifuged 20 minutes under 6000rpm, obtain supernatant I and precipitation I；
（7）Supernatant I is added to after mixing 14h in 4 DEG C of isometric cold ethanol, the 1/4 of filtering and concentrating to original volume, then It is freeze-dried, obtains microalgae powder i.e. livestock fodder additives；
（8）Microalgae powder is added in poultry feed, addition 13%, feeding poultry.
In the present embodiment, immobilized microalgae loading 60% in adsorption column；Microalgae density after culture is 4.0g/L.
Embodiment 4
The embodiment directly carries out grid algae cultivation not utilize the present invention using Wastewater from Pig Farm, is the comparative example of embodiment 1. Specific method is as follows：
Using the waste water culture grid algae on pig farm, start to collect when grid concentration of algae is more than 2.1g/L, precipitate, chlorella after precipitation Dry matter content be 15%.By grid algae and the maize peel that crushed 40 mesh sieves by weight 1:3 are mixed, and agent is pressed in blending process 150g/ tons addition bacillus subtilis (2.0 × 1010cfu/g) is measured, accumulates aerobic fermentation on ground after mixing, piling height is 100cm, in cone, bottom surface diameter of a circle is 2.0 meters.Environment temperature during fermentation is 25 DEG C.Ferment the 60th hour detection away from The height of ground 2/3, when reaching 60 DEG C away from surface of material 20cm depths fermentation temperature (temperature of charge reaches 72 DEG C at deep 25cm), this When material is opened from inside to outside after heap fermentation again, piling height is trapezoidal to be still 100cm, and bottom surface diameter of a circle is 2.0 Rice.The fermentation temperature at the 6th day (the 3rd day of second of heap fermentation) stacking material 20cm that ferments reach again 60 DEG C it is (now deep Temperature of charge reaches 74 DEG C at 25cm) when stop, then material is spread out and is cooled to 25 DEG C.
25 DEG C of grid algae material will be cooled to again with complete compound feed for fattening pig by weight 1:3 mixing, mixed process Middle addition Lactobacillus plantarum (content 2.0 × 1010Cfu/g, addition 200g/ ton), long handle trichoderma (content 1.5 × 109cfu/ G, addition 200g/ ton), regulation moisture to 50%, the material mixed is loaded into fermentation bag, anaerobism is carried out after compacting Ferment, fermented 3 days at 25 DEG C, detection pH (5% aqueous solution) 3.5, take out feeding growing and fattening pigs.
Each content of material such as table 1 in sewage after being handled in above example, microalgae dry weight such as table 2 after sewage disposal, in Empty fiber membrane performance such as table 3, protein content such as table 4 in microalgae after culture.
Each content of material in sewage after the processing of 1 each embodiment of table
Microalgae dry weight after each embodiment sewage disposal of table 2
Each embodiment doughnut film properties of table 3
Protein content in microalgae after each embodiment culture of table 4
By the data of embodiment 1 and embodiment 4 in table 1 ~ 4 as a comparison, compared to the method pair without using the present invention Sewage carries out processing and cultivates feed addictive prepared by microalgae, and protein content is high in feed addition prepared by the present invention, together When it is stronger to the detergent power of sewage, microalgae amount concentration is higher after processing.
The above embodiment of the present invention scheme is only the description of the invention and can not limit the present invention, in the power with the present invention Any change in sharp claim suitable implication and scope, is all considered to be and is included within the scope of the claims.

Claims (7)

  1. A kind of 1. method of fish-farming with sewage microalgae production livestock fodder additives, it is characterised in that comprise the following steps：
    （1）Band stomata snake pipe is installed at sewage reaction tank bottom, down, coiled pipe is connected stomata with ozone generating-device；
    （2）The preparation and installation of adsorption column：
    A. 50 parts of chitosans are weighed, is added in 60ml 95% ethanol solution and soaks, 60 DEG C of swelling 2h of constant temperature；By 150 parts of perfume (or spice) Oxalaldehyde is dissolved in 400ml 95% ethanol, and the soak for pouring into chitosan obtains mixed liquor；It is quick that mixed liquor is positioned over microwave In reaction unit, 70 DEG C of heating-up temperature is set, reacts 10min, microwave power 300w, stirring；Reaction is completed, cooled and filtered, Ethanol is washed, and washing, is dried under vacuum to constant weight, is obtained modification of chitosan powder；
    B. 20 parts of natural smectite is weighed, 5% sodium hydroxide of natural smectite quality is weighed, by solid-liquid mass ratio 1:10 add Enter 200ml distilled water, regulation pH is 5.6,60 DEG C of stirring reaction 1h in water bath with thermostatic control, the freeze-day with constant temperature at 80 DEG C, fine grinding 100 mesh sieve, it is modified montmorillonite used to obtain sodium；
    C. it is 10 to take cell concentration5/ ml microalgae solution, aseptically, with sodium alginate soln, modification of chitosan 1:1:1 It is well mixed to obtain mixed liquor；Mixed liquor is instilled into calcium chloride solution by syringe and carries out 20 ~ 30min of solidification, obtains marine alga Sour calcium coagulates pearl；Calcium alginate is coagulated into pearl and uses distillation water washing 2 ~ 3 times, you being fixed microalgae glueballs；
    The sodium alginate concentration is 20 ~ 25mg/ml；Described calcium chloride solution concentration is 18 ~ 22mg/ml；
    The mixed liquor is 1 with calcium chloride solution volume ratio：（1.5~2）；
    D. 40 ~ 80 parts of Kynoar powder is taken, is dissolved in 25 ~ 50 parts of dibutyl phthalates, adds 50 ~ 100 parts of modifications Chitosan powder, 50 ~ 100 parts of modified montmorillonite used powder, 2 ~ 10 parts of Geniposides, under nitrogen protection in 210 ~ 223 DEG C of oil baths After 4 ~ 6h of stirring is mixed into uniform solution, standing and defoaming obtains polymer；Polymer is fed by the Weightlessness balance of 5 ‰ measuring accuracies Material machine feeds double screw extruder, under 250 ~ 300rpm and 2 ~ 5MPa mixing kneading effect, material is sufficiently mixed uniformly Into the casting solution of stable homogeneous, casting solution enters shower nozzle by spinning pump with 90 ~ 100g/min speed accurate measurement, interior Chamber is using the nitrogen of 36 ~ 50ml/min flow velocitys as hollow supporting body, into 298K's after the air section evaporation of certain altitude Occur to be separated and solidify in water-bath, collected thick hollow-fibre membrane by collecting wheel with 25m/min speed；
    E. thick hollow-fibre membrane d being prepared is immersed in 95% industrial alcohol 24 hours, and extraction dibutyl phthalate is dilute Agent is released, during which need to change ethanol 2 times, it is ensured that diluent extraction is clean, and it is dry with clear water rinsing will to extract the hollow-fibre membrane finished It is net to obtain hollow-fibre membrane；
    F. hollow-fibre membrane is taken, is assembled into duplicature post, the wide spaces of 3 ~ 7cm are left among duplicature, outermost tunic bottom is set Dump valve is equipped with, obtains adsorption column；
    G. multiple adsorption columns are vertically fixed with brandreth, hollow space parallel connection in innermost layer is connected with suction pump, is positioned over reaction tank In；
    （3）Immobilized microalgae cell is filled in the intermediate space of adsorption column duplicature, seals adsorption column；
    （4）Open sewage pump and sewage is injected into reaction tank, when sewage is not to adsorption column 50 ~ 70%, stop pumping；Open ozone Generating means, ozone aeration quantity are 15 ~ 40g/h, and action time is 10 ~ 20min/h, after ozonization 5h, open suction pump, Regulation flow is 1 ~ 1.4L/min, 15 ~ 30min of working time, 40 ~ 60min of time of having a rest, sewage is entered adsorption column and fixation After change microalgae is reacted, reacted sewage is discharged into water purifying tank；
    （5）After operation 4 ~ 7 days, stopping is filled with ozone, stops sewage pump, stopped pumping pump, and immobilized microalgae is passed through into adsorption column Bottom-discharge valve is discharged, and is collected；Hollow-fibre membrane is cleaned 2 hours with 2 ~ 5wt%HCl, then with 2000 ~ 3000mg/L NaClO+ 1000 ~ 2000mg/L NaOH are cleaned 2 hours, and every component cleaning flux be 15 ~ 30L/h, 23 ~ 25 DEG C of temperature, eluent collection It is used to metal ion afterwards reclaim；
    （6）Collect immobilized microalgae and carry out broken wall treatment, it is using heated-air drying that microalgae is dry and crush, take microalgae powder 100 ~ 200 parts, add the cellulase that 10 ~ 20 parts of enzyme activities are 1400 ~ 1800U/g and the neutrality that enzyme activity is 60000 ~ 70000U/g Protease 1:1 combination, after mixing 4 ~ 6h of reaction, centrifuged 10 ~ 20 minutes under 5000 ~ 6000rpm, obtain supernatant I and precipitation I；
    （7）Supernatant I is added in 4 DEG C of isometric cold ethanol mix 12 ~ 14h after, filtering and concentrating to original volume 1/5 ~ 1/4, then it is freeze-dried, obtains microalgae powder i.e. livestock fodder additives.
  2. A kind of 2. method of fish-farming with sewage microalgae production livestock fodder additives according to claim 1, it is characterised in that： Solar energy LED lamp is provided with outside adsorption column, power supply is provided using solar energy power accumulating plate, is provided in rainy weather for micro algae growth Light source, the intensity of light source are 6000 ~ 10000lux.
  3. A kind of 3. method of fish-farming with sewage microalgae production livestock fodder additives according to claim 1, it is characterised in that： Immobilized microalgae loading 50 ~ 70% in adsorption column, adsorption column hollow space are not communicated with two membranes intermediate space part.
  4. A kind of 4. method of fish-farming with sewage microalgae production livestock fodder additives according to claim 1, it is characterised in that： The ozonization time is 5h/ days.
  5. A kind of 5. method of fish-farming with sewage microalgae production livestock fodder additives according to claim 1, it is characterised in that： Described Kynoar molecular weight is 30KDa ~ 40Kda, and Kynoar concentration is 30 ~ 35wt%.
  6. A kind of 6. method of fish-farming with sewage microalgae production livestock fodder additives according to claim 1, it is characterised in that： In the absorption column preparation method, prepare ecological film liquid bath temperature and be more than or equal to the bath temperature for preparing casting solution.
  7. A kind of 7. method of fish-farming with sewage microalgae production livestock fodder additives according to claim 1, it is characterised in that： Dibutyl phthalate, modification of chitosan, modified montmorillonite used powder ratio of weight and number are 1:2:2~2:2:5.
CN201710938494.1A 2017-10-11 2017-10-11 A kind of method of fish-farming with sewage microalgae production livestock fodder additives Active CN107487859B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710938494.1A CN107487859B (en) 2017-10-11 2017-10-11 A kind of method of fish-farming with sewage microalgae production livestock fodder additives

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710938494.1A CN107487859B (en) 2017-10-11 2017-10-11 A kind of method of fish-farming with sewage microalgae production livestock fodder additives

Publications (2)

Publication Number Publication Date
CN107487859A true CN107487859A (en) 2017-12-19
CN107487859B CN107487859B (en) 2018-06-12

Family

ID=60654391

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710938494.1A Active CN107487859B (en) 2017-10-11 2017-10-11 A kind of method of fish-farming with sewage microalgae production livestock fodder additives

Country Status (1)

Country Link
CN (1) CN107487859B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108244389A (en) * 2018-01-30 2018-07-06 广西壮族自治区畜牧研究所 It is a kind of using leaflet white popinac as broiler fodder of raw material and preparation method thereof
CN108783071A (en) * 2018-06-26 2018-11-13 安徽鲜森绿色食品有限公司 A kind of chicken feed addictive and its preparation process improving meat
CN108795768A (en) * 2018-04-19 2018-11-13 大连理工大学 A method of being aerated culture microalgae using film

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2338338Y (en) * 1997-06-10 1999-09-15 黄玉山 Micro-algae waste water treatment equipment
CN101525576A (en) * 2009-04-14 2009-09-09 中华绿油有限公司 Method for producing microalgae with domestic sewage and industrial sewage
CN102161550A (en) * 2011-01-13 2011-08-24 蔡志武 Method for producing feed additive from livestock and poultry breeding wastewater and purifying breeding wastewater to reclaimed water
CN103451091A (en) * 2013-09-18 2013-12-18 福州大学 Device for purifying livestock and poultry breeding wastewater by microalgae and method thereof
CN105753245A (en) * 2014-12-19 2016-07-13 宋维成 Method for treating poultry farm wastewater by using chlorella and chitosan
WO2016121007A1 (en) * 2015-01-27 2016-08-04 株式会社トーワ Feed additive for improving intestinal tract, and feed

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2338338Y (en) * 1997-06-10 1999-09-15 黄玉山 Micro-algae waste water treatment equipment
CN101525576A (en) * 2009-04-14 2009-09-09 中华绿油有限公司 Method for producing microalgae with domestic sewage and industrial sewage
CN102161550A (en) * 2011-01-13 2011-08-24 蔡志武 Method for producing feed additive from livestock and poultry breeding wastewater and purifying breeding wastewater to reclaimed water
CN103451091A (en) * 2013-09-18 2013-12-18 福州大学 Device for purifying livestock and poultry breeding wastewater by microalgae and method thereof
CN105753245A (en) * 2014-12-19 2016-07-13 宋维成 Method for treating poultry farm wastewater by using chlorella and chitosan
WO2016121007A1 (en) * 2015-01-27 2016-08-04 株式会社トーワ Feed additive for improving intestinal tract, and feed

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108244389A (en) * 2018-01-30 2018-07-06 广西壮族自治区畜牧研究所 It is a kind of using leaflet white popinac as broiler fodder of raw material and preparation method thereof
CN108244389B (en) * 2018-01-30 2020-09-01 广西壮族自治区畜牧研究所 Broiler feed taking albizzia julibrissin as raw material and preparation method thereof
CN108795768A (en) * 2018-04-19 2018-11-13 大连理工大学 A method of being aerated culture microalgae using film
CN108783071A (en) * 2018-06-26 2018-11-13 安徽鲜森绿色食品有限公司 A kind of chicken feed addictive and its preparation process improving meat

Also Published As

Publication number Publication date
CN107487859B (en) 2018-06-12

Similar Documents

Publication Publication Date Title
CN109534636B (en) Production system for comprehensively treating and utilizing waste straw and livestock and poultry manure
CN101307332B (en) Innocent comprehensive approach method for human feces by biotechnology
CN101549932B (en) Integrated production method of organic sewage/waste residue treatment, microalgae culturing and oil refining
US20100151558A1 (en) Tubular Microbial Growth System
CN106187341B (en) Cultivate excreta three grade fermemtation method
CN107487859B (en) A kind of method of fish-farming with sewage microalgae production livestock fodder additives
CN112077127A (en) Large-scale kitchen waste phase-change water production degradation treatment system and treatment method thereof
CN102515886B (en) Process for producing biological bacterial fertilizer by taking domestic sewage plant dewatered sludge as raw material
CN102874996A (en) Recovery and reutilization process of excrement and urine in livestock and poultry farm
CN108794075A (en) The feces of livestock and poultry base-material preparation method cured based on separating dry space from moist space and microbial deodorant
CN104987214A (en) Efficient biology leavening agent capable of decreasing pollution gas emission in urine immersing manure technology
CN108218480A (en) A kind of method and its system for handling human excrement
CN104163501B (en) A kind of method of utilizing complex microorganism nest to process liquid dung
CN102863116A (en) Method and device for repeatedly utilizing ramie biological degumming waste water
CN107487860A (en) A kind of fish-farming with sewage microalgae device and preparation method thereof
CN103962365B (en) The kitchen castoff treatment process of a kind of resource, innoxious, minimizing
CN108004132A (en) A kind of anaerobic ferment devices of recyclable microorganism ageing hydro-thermal charcoal and the recovery method of microorganism ageing hydro-thermal charcoal
CN112077126A (en) Household kitchen waste phase-change water production degradation treatment system and treatment method thereof
CN102765813A (en) Method for treating industrial and domestic sewage by active organisms
CN109111246A (en) A kind of quick manufacturing equipment of complex microorganism organic fertilizer
CN104846019A (en) Gas making method by mixing and fermenting cow dung separating medium and kitchen garbage
CN107585870B (en) A kind of method of microalgae treated sewage
CN102079663A (en) Ecologized medium-temperature high-efficiency compound fertilizer production system
CN107226596A (en) A kind of technique for being engineered bacterium algae fermentation herding liquid dung and Collocation cultivation water
CN104312899B (en) A kind of aerobic-anaerobic intermittent aerating reactor for preparing nanometer selenium and its method for preparing nanometer selenium

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Guo Haitao

Inventor after: Shi Hang

Inventor before: Qin Yangyang

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20180504

Address after: 266300 Shandong Qingdao Jiaozhou Yunxi Street office Da Xi Zhuang Village

Applicant after: Qingdao Edfu Biotechnology Co., Ltd.

Address before: 530000 No. 409, Haotian Plaza, 24 Long Lake Road, Qingxiu District, Nanning, the Guangxi Zhuang Autonomous Region

Applicant before: Guangxi Nanning Rong Weide Amperex Technology Limited

GR01 Patent grant
GR01 Patent grant