CN104163501B - A kind of method of utilizing complex microorganism nest to process liquid dung - Google Patents

A kind of method of utilizing complex microorganism nest to process liquid dung Download PDF

Info

Publication number
CN104163501B
CN104163501B CN201410396029.6A CN201410396029A CN104163501B CN 104163501 B CN104163501 B CN 104163501B CN 201410396029 A CN201410396029 A CN 201410396029A CN 104163501 B CN104163501 B CN 104163501B
Authority
CN
China
Prior art keywords
liquid dung
complex
complex microorganism
bacillus subtilis
described step
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201410396029.6A
Other languages
Chinese (zh)
Other versions
CN104163501A (en
Inventor
刘务彪
李有志
潘登
张淑二
宫献治
刘丽芬
张涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
The peaceful thing Engineering Co., Ltd in Shandong hundred million
Original Assignee
JINAN YIAN BIOLOGY RESEARCH INSTITUTE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by JINAN YIAN BIOLOGY RESEARCH INSTITUTE filed Critical JINAN YIAN BIOLOGY RESEARCH INSTITUTE
Priority to CN201410396029.6A priority Critical patent/CN104163501B/en
Publication of CN104163501A publication Critical patent/CN104163501A/en
Application granted granted Critical
Publication of CN104163501B publication Critical patent/CN104163501B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a kind of complex microorganism nest that utilizes and process the method for liquid dung, comprise the steps: that (1) leaves standstill pretreatment by liquid dung under the condition of isolating oxygen not, makes pretreatment liquid dung; (2) first lay initial bed, then lay padding layer at initial bed upper surface, then spill complex micro organism fungicide to padding layer upper surface paving, after then batch mixing being mixed with bacterium liquid, paving is spilt on complex micro organism fungicide, standing for fermentation, makes complex microorganism nest; (3) pretreatment liquid dung is passed into the upper surface of complex microorganism nest, fermentation, by the complex microorganism nest after fermentation through pulverizing, granulation, make organic fertilizer. The present invention adopts complex microorganism nest to process liquid dung as main thermal source first, and the main moisture in liquid dung is entered in air in the mode of steam, and environmentally safe has solved again the dry problem of liquid dung, does not need to add other energy substances.

Description

A kind of method of utilizing complex microorganism nest to process liquid dung
Technical field
The present invention relates to a kind of method of utilizing complex microorganism nest to process liquid dung, belong to the dirty comprehensive utilization technique of herding excrement field.
Background technology
The primary pollution source that animal husbandry and urban life produce without the liquid dung of processing. Liquid dung produces foul smell, has a strong impact on weekEnclose masses' normal life and work; Liquid dung has eutrophication, flows into the surface water aquatic organism survival rates such as river and declines, contaminant waterBody; The liquid dung water that permeates the ground, pollutes impact and drinks; Liquid dung be directly discharged into farmland can continue fermentation cause Plants burn root,Soil pH value raises and causes basic soil.
The general liquid dung moisture content without processing, 80~95%, cultivates following (mg/L): the BOD of excrement sewage matter indication range5:1200-15300, CODCr:2720-31000, SS:160-20500; Be subject to the excrement of livestock and poultry without the liquid dung moisture content of processingThe impact of urinary excretion, age, body weight, physiological stage, diet composition, environment temperature etc. As the age and body weight less, rowDischarge is less; Lactation period livestock and poultry compared with period of pregnancy feed intake large, excretion is also large; When diet roughage content is higher, the adopting of livestock and poultryHeavy, digestibility is low, and excretion is large; Summer environment temperature is high, and the amount of drinking water of livestock and poultry is large, and the amount of urinating is also many. Livestock and poultry fecaluriaGeneration also relevant with feeding and management technique with character: dry clear excrement technique fecaluria does not substantially mix in poultry house, and excrement can be independentClear out house, the water content of excrement is in 80% left and right, and this excrement becomes argol; If fecaluria mixes in house, excrement water content is 90%Left and right, is called semifluid excrement; Bubble excrement or water rush excrement water content more than 90%, are called liquid excrement. The generation of three kinds of excrementComputational methods are not identical: argol is only counted fresh excrement amount or fresh excrement and bedding and padding total amount; Semifluid excrement can be by fecaluria total amount; Liquid excrementShould comprise excrement, urine and industrial water amount.
Due to the These characteristics of liquid dung, cause it to utilize difficulty larger, at present main employing utilizes mode as follows:
1, filtrate directly or is after filtering as the raw material that produces biogas, as Chinese patent literature CN103191904A (ShenPlease numbers 201310126581.9) a kind of pig farm dejecta treatment method is disclosed, comprise by the excreta of pig be separated into argol andLiquid dung, argol is done after fermentation process respectively as raw material and the fish meal of producing compoiste fertilizer, and described liquid dung is divided into two parts placeReason, a part of liquid dung enters methane-generating pit for the production of biogas, and another part liquid dung is sent into sedimentation basin for precipitating sterilization, liquid dung warpPrecipitation disinfect after for cleaning pig house. The major defect of the method is: have high input, utilization rate is low, and northern area is inapplicable,The energy obtaining cannot utilize, and cannot obtain cultivating surrounding enviroment and purify, raw material is not reproducible utilize follow-up needs constantly drop into,Price is high, and the seepage water of filtration need to continue to process secondary and drop into, and operating cost is high.
2, the serial initial condition processing such as precipitation, aerobic, anaerobism, as Chinese patent literature CN101654318A (application number200910092443.7) disclose a kind of method of night soil treatment, it comprises the following steps: Separation of Solid and Liquid, flocculation dewatering,The Aerobic Process for Treatment of the Anaerobic Treatment of aerobic compost, sewage, the double oxygen processing of sewage, sewage, the membrane biological reaction of sewage process andStink is administered. The shortcoming of the method is: have high input, operating cost is high, cannot minimizing, resource, cannot obtain cultivation weekSurrounding environment purifies.
3, be directly made into organic commercial fertilizer, as Chinese patent literature CN103848649A (application number 201210504442.0)The fertilizer that discloses production method, equipment and production thereof that a kind of human faecal mass is converted into fertilizer, method comprises the following steps: one,Biogas in septic tank is extracted by extraction machine, and solid, liquid ight soil in septic tank is as original slurry; Two,Original slurry is filtered by filter, remove foreign material, obtain preliminary ight soil slurry; Three, preliminary ight soil slurry is addedAdd drier and be uniformly mixed in mixer, obtain the half-dried slag of ight soil; Four, biogas is done through gas and water separatorDry; Five, dried biogas is burnt, the half-dried slag of ight soil that utilizes the heat of biogas combustion that step 3 is obtained is in evaporationIn device, carry out heating evaporation, obtain the dry slag of ight soil. The shortcoming of the method is: dry is utilized, and the water after pollution cannotProcess.
Therefore, provide a kind of method of comprehensive utilization of liquid dung, prevent secondary pollution, become the crucial difficult point of processing liquid dung.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, provide a kind of method of utilizing complex microorganism nest to process liquid dung, the method energyRealize the environment of minimizing, innoxious, resource, purifying contaminated source periphery.
Technical solution of the present invention is as follows:
Utilize complex microorganism nest to process a method for liquid dung, comprise the steps:
(1) liquid dung is left standstill under the condition of isolating oxygen not to pretreatment 24~48 hours, make pretreatment liquid dung;
(2) first lay initial bed, initial bed thickness is 100~160cm, then lays padding layer at initial bed upper surface, padThickness of feed layer is 8~20cm, then spills complex micro organism fungicide, complex micro organism fungicide amount of application to padding layer upper surface paving0.5~2kg/m2, then by batch mixing and bacterium liquid (3~6) in mass ratio: after 1 ratio is mixed, by 0.3~1kg/m2ExecuteConsumption paving is spilt on complex micro organism fungicide, and standing for fermentation 60~90h, makes complex microorganism nest;
Described complex micro organism fungicide is the mixing of aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis;
Described bacterium liquid is the bacterium liquid that aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis mix, and in bacterium liquid, cell concentration is >=10 hundred millioncfu/ml;
Bed material is density 0.096~0.16g/cm3, water absorption rate 15~60% the agricultural that contains cellulose, starch, protein uselessGurry, bedding and padding are density 0.2~0.4g/cm3, water absorption rate 15~65% the agricultural that contains cellulose, starch, protein uselessGurry, batch mixing is density 0.45~0.55g/cm3The agricultural wastes that contain cellulose, starch, protein;
(3) pretreatment liquid dung step (1) being made passes into the upper surface of the complex microorganism nest that step (2) makes, pre-The ratio of processing liquid dung intake volume and complex microorganism nest volume is 1:(45~55), ferment 30~100 days, will fermentAfter complex microorganism nest through pulverize, granulation, make organic fertilizer.
Preferred according to the present invention, in described step (1), be also included in to leave standstill and add complex micro organism fungicide before pretreatmentStep, described complex micro organism fungicide is the mixing of aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis, every gram of complex microorganism containsThe ading up to of some mentioned microorganisms >=1,000,000,000.
Further preferred according to the present invention, in described step (1), aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis mixedComposition and division in a proportion example is 1:(0.5~5): (1~7.5); Further preferred, in described step (1), complex micro organism fungicideAddition is 0.5 of liquid dung quality~5 ‰.
Further preferred according to the present invention, in described step (1), aspergillus oryzae is that the open and flat mutation of aspergillus oryzae, aspergillus oryzae are dredged exhibitionMutation; Saccharomyces cerevisiae is the oval mutation of saccharomyces cerevisiae; Bacillus subtilis is bacillus subtilis withered grass subspecies, bacillus subtilisBacterium Si Shi subspecies;
Preferred according to the present invention, in described step (2), the mixed proportion of aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilisFor 1:(0.5~5): (1~7.5).
Preferred according to the present invention, in described step (2), aspergillus oryzae is the open and flat mutation of aspergillus oryzae, A. oryzae var. effusus;Saccharomyces cerevisiae is the oval mutation of saccharomyces cerevisiae; Bacillus subtilis is bacillus subtilis withered grass subspecies, bacillus subtilis Si ShiSubspecies.
Preferred according to the present invention, in described step (2), bacterium liquid adopts aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis singleSolely the zymotic fluid after liquid fermentation proportionally mixes and makes.
Preferred according to the present invention, in described step (2), after standing for fermentation 60~90h, complex microorganism nest surface 20 public affairsTemperature below point reaches more than 60 DEG C.
Preferred according to the present invention, in described step (2), bed material is the crushed material of rice husk, corncob, crop material, grainDegree 5~10mm, 2.5~5mm, thick 20~30 μ m; Bedding and padding are the crushed material of sawdust, chaff or waste edible fungus gurry, granularity5~10mm, 2.5~5mm, thick 20~300 μ m; Batch mixing is wheat bran, seawood meal or bean cake powder, granularity 5~10mm, 2.5~5mm, thick 20~300 μ m.
Preferred according to the present invention, in described step (2), the length of complex microorganism nest is 4~8m, and wide is 4~8m, is deeply1.1~2.0m。
Preferred according to the present invention, in described step (3), it is every square metre 0.1~3 per hour that pretreatment liquid dung passes into speedTon.
Preferred according to the present invention, in described step (3), be crushed to 40 orders.
Preferred according to the present invention, in described step (3), in sweat, every 10~20h, turn over throwing once.
The present invention makes full use of aspergillus oryzae to be had efficient amylase formation efficiency, saccharomyces cerevisiae and has efficient cellulase and generateEfficiency, bacillus subtilis have efficient protease formation efficiency, and three kinds of bacterium cooperatively interact, and have the growth of mutually promoting, heightEffect is decomposed the effect of agricultural wastes heat production.
Above-mentioned processing step if no special instructions, all can adopt this area common process condition.
Liquid dung, after complex microorganism nest, only has the percolate of minute quantity and contains mass efficient bacterium, and percolate, after collecting, returnsFlow in biological nest and participate in again fermenting.
Beneficial effect of the present invention is as follows:
1, the present invention adopts complex microorganism nest to process liquid dung as main thermal source first, and microorganism utilizes dissolved organic matter notDisconnected propagation, the energy producing in conversion with in utilizing the process of chemical energy has exceeded the synthetic institute of cell energy requirement, adds the guarantor of materialTemperature effect, temperature constantly rises, and causes moisture evaporation, and the main moisture in liquid dung is entered in air in the mode of steam,Environmentally safe has solved again the dry problem of liquid dung, does not need to add other energy substances;
2, complex microorganism nest of the present invention utilization contains cellulosic plant husbandry discarded object and forestry processing waste providesCarbon source, utilization can produce that protease, cellulase, diastatic aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis mixesComplex micro organism fungicide carries out mixed culture fermentation, the collocation of these three kinds of bacterium the growth of not only can mutually promoting, and can rapid, high volumeProduce heat, thereby provide thermal source for processing liquid dung;
3, the present invention carries out pretreatment to liquid dung, makes the microorganism of adding tentatively adapt to the environment in liquid dung, thereby it is being enteredEnter in the step of complex microorganism nest, energy fast decoupled organic matter, produces heat;
4, micro-complex microorganism nest of the present invention passes through reasonably optimizing constituent and raw material, thereby is composite microbialThing nest is quick, sustained release heat provides safeguard;
5, the raw materials used wide material sources of the present invention, the simple expense of constructing is low, without foul smell, pollution-free, safeguard simple, power consumption less,Widely applicable, and the organic fertilizer finally making can make up post sales the purchasing of raw materials and bacterial classification buying expenses, and have 30% left and rightProfit space, thereby provide economic base for popularization of the present invention.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme of the present invention is described further, but institute of the present invention protection domain is not limited to this.
Embodiment 1
Raw material explanation
Rice husk: require without going mouldy, antibiotic-free, shape be complete; Density 0.13~0.15g/cm3, water absorption rate 35~40%;
Wheat bran: require without going mouldy, antibiotic-free, density 0.45~0.50g/cm3
Sawdust: require preservative free, glue-free, density 0.25~0.28g/cm3, water absorption rate 50~55%;
Complex micro organism fungicide is the probiotics that Shandong hundred million peaceful thing Engineering Co., Ltd produces, and contains product starch in this microbial inoculumThe aspergillus oryzae of enzyme, the saccharomyces cerevisiae of cellulase-producing and the bacillus subtilis that produces protease, aspergillus oryzae, saccharomyces cerevisiae and withered grassThe mixed proportion of bacillus is 1:2:3; Add up to >=1,000,000,000 cfu of the mentioned microorganism that every gram of complex microorganism contains;
Liquid dung: derive from Yangling Besun Agricultural Industry Group Co., Ltd., moisture content 85~98%, the storage time is less than 30 days,Without bactericide;
Utilize complex microorganism nest to process the method for liquid dung, comprise the steps:
(1) in liquid dung, add complex micro organism fungicide, under the condition of isolating oxygen not, pretreatment 36 hours, makes pretreatmentLiquid dung;
The addition of complex micro organism fungicide is 3 ‰ of liquid dung weight;
(2) first lay initial bed, initial bed thickness is 130cm, then lays padding layer, bedding and padding bed thickness at initial bed upper surfaceDegree is 18cm, then spills complex micro organism fungicide, complex micro organism fungicide amount of application 1kg/m to padding layer upper surface paving2, soAfter after by batch mixing, the ratio of 4:1 is mixed in mass ratio with bacterium liquid, by 0.8kg/m2Amount of application paving spill on complex micro organism fungicide,Standing for fermentation 72h, the following temperature in 20 centimeters, complex microorganism nest surface reaches more than 60 DEG C, makes complex microorganism nest;
(3) pretreatment liquid dung step (1) being made is evenly squeezed into the upper surface of the complex microorganism nest making. (2) locate in advanceReason liquid dung admission velocity is 60 tons/hour, and the ratio of pretreatment liquid dung inlet volume and complex microorganism nest volume is 1:50,Ferment 60 days. (3) by fermentation after complex microorganism nest through being crushed to 40 orders, granulation, make organic fertilizer.
In described step (2), bed material is rice husk, and bedding and padding are sawdust, and batch mixing is wheat bran;
In above-mentioned steps (2), the length of complex microorganism nest is 8m, and wide is 4m, is 1.5m deeply.
Course of reaction principle of the present invention is as follows:
Add complex micro organism fungicide to liquid dung, the aspergillus oryzae in complex micro organism fungicide, saccharomyces cerevisiae, bacillus subtilis canTo produce protease, cellulase, amylase, by pretreatment, not only make the microorganism adding tentatively adapt to liquid dung environment,And the larger molecular organics in liquid dung has been carried out to preliminary decomposition, the microorganism that is conducive to next step utilizes;
Evenly be sprayed to complex microorganism nest surface with sewage pump or drop, complex microorganism nest inside through pretreated liquid dung(distance surface 20 centimeters following) temperature is for being greater than 60 DEG C, often through 10 hours, complex microorganism nest turned over to throwing, greatlyThe moisture evaporation of approximately 97% left and right, the aspergillus oryzae in complex microorganism nest, saccharomyces cerevisiae, bacillus subtilis can efficiently produceProtease, cellulase, amylase, it is micromolecular monose by macromolecules starch class substance decomposition that amylase is used for; CelluloseEnzyme is for being degraded to small molecular sugar by cellulose substances; Protease effect is that the protein matter in excrement dirt is degraded into little moleculeAmino acids; Larger molecular organics is decomposed into small-molecule substance by these enzymes, further promotes mentioned microorganism growth, heat production,For the evaporation of moisture in liquid dung provides thermal source.
Final complex microorganism nest is played heap fermentation about 3 days through microorganism, makes organic fertilizer through pulverizing, plasmid.
Detection method: 1. count plate: the method for plate culture count. 2..CODcr: potassium dichromate method. 3, organic total amount:Potassium bichromate titrimetric method.
After testing, viable bacteria concentration 0.5 hundred million cfu/ grams, organic >=45% in the organic fertilizer making, total nutrient >=5.0.
The organic fertilizer obtaining can obtain 350 yuan/ton of incomes by commercially available price, removes raw material and microbial inoculum cost, one ton of every processingProfitable 80~100 yuan/ton of liquid dung, thus the ecology and economy income of dung dispose obtained first.
Embodiment 2
Raw material explanation
Rice husk: require without going mouldy, antibiotic-free, shape be complete; Density 0.15~0.16g/cm3, water absorption rate 15~30%;
Wheat bran: require without going mouldy, antibiotic-free, shape be complete; Density 0.45~0.50g/cm3
Sawdust: require preservative free, without watering, antibiotic-free; Density 0.35~0.4g/cm3, water absorption rate 15~20%;
Complex micro organism fungicide:
A. oryzae var. effusus (Aspergillusoryzaevar.effusus), purchased from Chinese common micro-organisms preservation managementCenter, deposit number 3.521;
Saccharomyces cerevisiae (Saccharomycescerevisiae), purchased from Chinese common micro-organisms preservation administrative center, deposit number2.3888;
Bacillus subtilis withered grass subspecies (Bacillussubtilissubsp.subtilis), purchased from Chinese common micro-organismsPreservation administrative center, deposit number 1.2162;
After above-mentioned bacterial classification is fermented by prior art respectively, mix than the ratio of 1:0.5:1 in thalline quantity, make multipleClose microbial bacterial agent, the mentioned microorganism that every gram of complex microorganism contains add up to 1,000,000,000 cfu;
Liquid dung: derive from Yangling Besun Agricultural Industry Group Co., Ltd., moisture content 85~88%, the storage time is less than 30 days,Without bactericide;
Utilize complex microorganism nest to process a method for liquid dung, comprise the steps:
(1) in liquid dung, add complex micro organism fungicide, under the condition of isolating oxygen not, pretreatment 24 hours, makes pretreatmentLiquid dung;
The addition of complex micro organism fungicide is 0.5 ‰ of liquid dung quality.
(2) first lay initial bed, initial bed thickness is 100cm, then lays padding layer, bedding and padding bed thickness at initial bed upper surfaceDegree is 8cm, then spills complex micro organism fungicide, complex micro organism fungicide amount of application 0.5kg/m to padding layer upper surface paving2, soAfter after by batch mixing, the ratio of 3:1 is mixed in mass ratio with bacterium liquid, by 0.3kg/m2Amount of application paving spill on complex micro organism fungicide,Standing for fermentation 90h, the following temperature of charge in 20 centimeters, complex microorganism nest surface reaches 60 DEG C, makes complex microorganism nest;
(3) pretreatment liquid dung step (1) being made passes into the upper surface of the complex microorganism nest that step (2) makes, pre-It is 60 tons/hour that processing liquid dung passes into speed, and the ratio of pretreatment liquid dung intake volume and complex microorganism nest volume is 1:45, ferment 30 days, the complex microorganism nest after fermentation, through being crushed to 40 orders, granulation, is made to organic fertilizer.
In above-mentioned steps (2), the length of complex microorganism nest is 8m, and wide is 4m, is 1.1m deeply.
Detection method: 1. count plate: the method for plate culture count. 2.BOD5 differential direct-reading BOD analyzer. 3.CODcr:Potassium dichromate method. 4. organic total amount: potassium bichromate titrimetric method. 5. suspension: gravimetric method.
After testing, viable bacteria concentration 0.5 hundred million cfu/ grams, quality of organic matter mark >=40% in the organic fertilizer making, total nutrient >=4.0。
The organic fertilizer obtaining can obtain 350 yuan of incomes by commercially available price, removes raw material and microbial inoculum cost, one ton of liquid dung of every processingProfitable 80~100 yuan, thus the ecology and economy income of dung dispose obtained first.
Embodiment 3
Raw material explanation
The crushed material of peanut stalk: require without going mouldy, antibiotic-free, shape be complete; Density 0.096~0.12g/cm3, water suctionRate 55~60%;
Bean cake powder: require without going mouldy, antibiotic-free, shape be complete; Density 0.50~0.55g/cm3
Chaff: require preservative free, without watering, antibiotic-free; Density 0.2~0.25g/cm3, water absorption rate 60~65%;
Complex micro organism fungicide:
Aspergillus oryzae (Aspergillusoryzae), purchased from Chinese common micro-organisms preservation administrative center, deposit number 3.5232;
Saccharomyces cerevisiae (Saccharomycescerevisiae), purchased from Chinese common micro-organisms preservation administrative center, deposit number2.3875;
Bacillus subtilis Si Shi subspecies (Bacillussubtilissubsp.spizizenii), purchased from the common micro-life of ChinaThing preservation administrative center, deposit number 1.1849;
After above-mentioned bacterial classification is fermented by prior art respectively, mix than the ratio of 1:5:7.5 in thalline quantity, make compoundMicrobial bacterial agent, the mentioned microorganism that every gram of complex microorganism contains add up to 1,000,000,000 cfu;
Liquid dung: derive from Yangling Besun Agricultural Industry Group Co., Ltd., moisture content 90~98%, the storage time is less than 30 days,Without bactericide;
Utilize complex microorganism nest to process a method for liquid dung, comprise the steps:
(1) in liquid dung, add complex micro organism fungicide, pretreatment 24~48 hours under the condition of isolating oxygen not, makes pre-Process liquid dung;
The addition of complex micro organism fungicide is 5 ‰ of liquid dung weight.
(2) first lay initial bed, initial bed thickness is 160cm, then lays padding layer, bedding and padding bed thickness at initial bed upper surfaceDegree is 20cm, then spills complex micro organism fungicide, complex micro organism fungicide amount of application 2kg/m to padding layer upper surface paving2, soAfter after by batch mixing, the ratio of 6:1 is mixed in mass ratio with bacterium liquid, by 1kg/m2Amount of application paving spill on complex micro organism fungicide,Standing for fermentation 60h, the following temperature of charge in 20 centimeters, complex microorganism nest surface reaches 70 DEG C, makes complex microorganism nest;
(3) pretreatment liquid dung step (1) being made passes into the upper surface of the complex microorganism nest that step (2) makes, pre-It is 60 tons/hour that processing liquid dung passes into speed, and the ratio of pretreatment liquid dung intake volume and complex microorganism nest volume is 1:55, ferment 100 days, the complex microorganism nest after fermentation, through being crushed to 40 orders, granulation, is made to organic fertilizer.
In above-mentioned steps (2), the length of complex microorganism nest is, 8m, and wide is 4m, is 2.0m deeply.
Detection method: 1. count plate: the method for plate culture count. 2.BOD5 differential direct-reading BOD analyzer. 3.CODcr:Potassium dichromate method. 4. organic total amount: potassium bichromate titrimetric method. 5. suspension: gravimetric method.
After testing, viable bacteria concentration 0.5 hundred million cfu/ grams, quality of organic matter mark >=45% in the organic fertilizer making, total nutrient >=4.5。
The organic fertilizer obtaining can obtain 350 yuan of incomes by commercially available price, removes raw material and microbial inoculum cost, one ton of liquid dung of every processingProfitable 80~100 yuan, thus the ecology and economy income of dung dispose obtained first.

Claims (13)

1. utilize complex microorganism nest to process a method for liquid dung, it is characterized in that, comprise the steps:
(1) liquid dung is left standstill under the condition of isolating oxygen not to pretreatment 24~48 hours, make pretreatment liquid dung;
In described step (1), also be included in and leave standstill the step of adding complex micro organism fungicide before pretreatment, described complex micro organism fungicide is the mixing of aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis, the ading up to of the mentioned microorganism that every gram of complex microorganism contains >=1,000,000,000;
(2) first lay initial bed, initial bed thickness is 100~160cm, then lays padding layer at initial bed upper surface, and padding layer thickness is 8~20cm, then spills complex micro organism fungicide, complex micro organism fungicide amount of application 0.5~2kg/m to padding layer upper surface paving2, then by batch mixing and bacterium liquid (3~6) in mass ratio: after 1 ratio is mixed, by 0.3~1kg/m2Amount of application paving spill on complex micro organism fungicide, standing for fermentation 60~90h, makes complex microorganism nest;
Described complex micro organism fungicide is the mixing of aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis;
Described bacterium liquid is the bacterium liquid that aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis mix, and in bacterium liquid, cell concentration is >=10 hundred million cfu/ml;
Bed material is density 0.096~0.16g/cm3, water absorption rate 15~60% the agricultural wastes that contain cellulose, starch, protein, bedding and padding are density 0.2~0.4g/cm3, water absorption rate 15~65% the agricultural wastes that contain cellulose, starch, protein, batch mixing is density 0.45~0.55g/cm3The agricultural wastes that contain cellulose, starch, protein;
(3) pretreatment liquid dung step (1) being made passes into the upper surface of the complex microorganism nest that step (2) makes, the ratio of pretreatment liquid dung intake volume and complex microorganism nest volume is 1:(45~55), ferment 30~100 days, complex microorganism nest after fermentation, through pulverizing, granulation, is made to organic fertilizer.
2. the method for claim 1, is characterized in that, in described step (1), the mixed proportion of aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis is 1:(0.5~5): (1~7.5).
3. the method for claim 1, is characterized in that, in described step (1), the addition of complex micro organism fungicide is 0.5 of liquid dung quality~5 ‰.
4. the method for claim 1, is characterized in that, in described step (1), aspergillus oryzae is the open and flat mutation of aspergillus oryzae, A. oryzae var. effusus; Saccharomyces cerevisiae is the oval mutation of saccharomyces cerevisiae; Bacillus subtilis is bacillus subtilis withered grass subspecies, bacillus subtilis Si Shi subspecies.
5. the method for claim 1, is characterized in that, in described step (2), the mixed proportion of aspergillus oryzae, saccharomyces cerevisiae and bacillus subtilis is 1:(0.5~5): (1~7.5).
6. the method for claim 1, is characterized in that, in described step (2), aspergillus oryzae is the open and flat mutation of aspergillus oryzae, A. oryzae var. effusus; Saccharomyces cerevisiae is the oval mutation of saccharomyces cerevisiae; Bacillus subtilis is bacillus subtilis withered grass subspecies, bacillus subtilis Si Shi subspecies.
7. the method for claim 1, is characterized in that, in described step (2), bacterium liquid adopts the zymotic fluid after aspergillus oryzae, saccharomyces cerevisiae and the independent liquid fermentation of bacillus subtilis proportionally to mix and make.
8. the method for claim 1, is characterized in that, in described step (2), after standing for fermentation 60~90h, the following temperature in 20 centimeters, complex microorganism nest surface reaches more than 60 DEG C.
9. the method for claim 1, is characterized in that, in described step (2), bed material is the crushed material of rice husk, corncob, crop material, granularity 5~10mm, 2.5~5mm, thick 20~30 μ m; Bedding and padding are the crushed material of sawdust, chaff or waste edible fungus gurry, granularity 5~10mm, 2.5~5mm, thick 20~300 μ m; Batch mixing is wheat bran, seawood meal or bean cake powder, granularity 5~10mm, 2.5~5mm, thick 20~300 μ m.
10. the method for claim 1, is characterized in that, in described step (2), the length of complex microorganism nest is 4~8m, and wide is 4~8m, is 1.1~2.0m deeply.
11. the method for claim 1, is characterized in that, in described step (3), it is 0.1~0.5 ton every square metre per hour that pretreatment liquid dung passes into speed.
12. the method for claim 1, is characterized in that, in described step (3), are crushed to 40 orders.
13. the method for claim 1, is characterized in that, in described step (3), in sweat, every 10~20h, turn over throwing once.
CN201410396029.6A 2014-08-12 2014-08-12 A kind of method of utilizing complex microorganism nest to process liquid dung Active CN104163501B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410396029.6A CN104163501B (en) 2014-08-12 2014-08-12 A kind of method of utilizing complex microorganism nest to process liquid dung

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410396029.6A CN104163501B (en) 2014-08-12 2014-08-12 A kind of method of utilizing complex microorganism nest to process liquid dung

Publications (2)

Publication Number Publication Date
CN104163501A CN104163501A (en) 2014-11-26
CN104163501B true CN104163501B (en) 2016-05-18

Family

ID=51907510

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410396029.6A Active CN104163501B (en) 2014-08-12 2014-08-12 A kind of method of utilizing complex microorganism nest to process liquid dung

Country Status (1)

Country Link
CN (1) CN104163501B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106082453A (en) * 2016-08-02 2016-11-09 济南亿安生物研究所 A kind of takeup type isoconcentration sprays metering device and application thereof
CN106281535B (en) * 2016-08-31 2019-05-10 甘肃祁牧乳业有限责任公司 A kind of preparation process of excrement of animals biomass fuel
CN108203317A (en) * 2016-12-20 2018-06-26 中国科学院城市环境研究所 A kind of feces of livestock and poultry cooperates with consumption technology with breeding wastewater
CN107601680A (en) * 2017-10-24 2018-01-19 临沂市畜牧站 The dirty method of biofermentation harmless treatment excrement

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101773075A (en) * 2010-02-11 2010-07-14 黄艳萍 Method for preparing dry scattering type ecological fermenting bed
CN103416315A (en) * 2013-07-17 2013-12-04 李宏江 Novel washing-free and zero-emission device for raising pigs and application method of bedding materials of novel washing-free and zero-emission device
CN104221886A (en) * 2014-10-10 2014-12-24 安徽万利生态园林景观有限公司 Bed padding special for raising pigs and manufacturing method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101773075A (en) * 2010-02-11 2010-07-14 黄艳萍 Method for preparing dry scattering type ecological fermenting bed
CN103416315A (en) * 2013-07-17 2013-12-04 李宏江 Novel washing-free and zero-emission device for raising pigs and application method of bedding materials of novel washing-free and zero-emission device
CN104221886A (en) * 2014-10-10 2014-12-24 安徽万利生态园林景观有限公司 Bed padding special for raising pigs and manufacturing method thereof

Also Published As

Publication number Publication date
CN104163501A (en) 2014-11-26

Similar Documents

Publication Publication Date Title
CN101307332B (en) Innocent comprehensive approach method for human feces by biotechnology
CN106754461B (en) Biological floc and preparation method and application thereof
CN102161550B (en) Method for producing feed additive from livestock and poultry breeding wastewater and purifying breeding wastewater to reclaimed water
US20190300407A1 (en) Biological Floc and Uses Thereof, for example, in Sludge Innocent Treatment and Industrial Water Purification Treatment
CN102584364B (en) Biologic organic fertilizer and preparation method thereof
CN101955382B (en) Treatment method and treatment system of food wastes
CN102010109B (en) Application of bio-enzyme catalysis in residual sludge treatment
CN100387551C (en) Method of producing active nutritional fertilizer using old domostic garbage
CN103467143B (en) Airtight aerobic production process of bio-organic fertilizer by municipal sludge or kitchen biogas residues
CN106986720A (en) The method for producing soil conditioner using the waste residue for handling livestock breeding wastewater generation
CN101244955B (en) Biological fertilizer and manufacture method thereof
CN103937695B (en) A kind of composite bacteria agent and manufacture method thereof of processing livestock and poultry cultivation sewage
CN102603383B (en) Production equipment and preparation method of slow-release bio-organic fertilizer
CN102336508A (en) Method and system for quickly dehydrating and recycling municipal sewage sludge
CN104212840A (en) Treatment method of antibiotic fermenting bacterial residues
CN104163501B (en) A kind of method of utilizing complex microorganism nest to process liquid dung
CN106508820A (en) Method for processing sludge, kitchen waste and straw from village
CN106135884A (en) Utilize agricultural wastes produced environment friendly function ferment and preparation method thereof
CN103664287A (en) Biological organic fertilizer prepared from water plants
CN108405561A (en) A kind of kitchen garbage recovery processing technique
CN105439776A (en) Fermentation treatment method for kitchen residues
CN114988926A (en) Organic compound fertilizer produced by eighty percent of sludge with water content and manufacturing method thereof
CN108585972A (en) A kind of green multiple stage circulation of discarded citrus utilizes method
CN111153749A (en) Agricultural waste treatment processing technology
CN109796113A (en) A kind of sludge and derivative manufacturing method of high speed processing moisture content 80 percent

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information

Inventor after: Liu Wubiao

Inventor after: Li Youzhi

Inventor after: Pan Deng

Inventor after: Zhang Shuer

Inventor after: Gong Xianzhi

Inventor after: Liu Lifen

Inventor after: Zhang Tao

Inventor before: Liu Wubiao

Inventor before: Chang Zhong

Inventor before: Pan Deng

Inventor before: Zhou Jianping

Inventor before: Meng Qinghua

Inventor before: Shi Xiaojie

COR Change of bibliographic data
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20180810

Address after: 250013 Room 301, building 9, zone D, 108 Ring Road, Lixia District, Ji'nan, Shandong.

Patentee after: The peaceful thing Engineering Co., Ltd in Shandong hundred million

Address before: 250013 Room 303, building 9, zone D, 108 Ring Road, Lixia District, Ji'nan, Shandong.

Patentee before: JINAN YIAN BIOLOGY RESEARCH INSTITUTE

CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Li Youzhi

Inventor after: Liu Wubiao

Inventor after: Zhang Shuer

Inventor after: Pan Deng

Inventor after: Gong Xianzhi

Inventor after: Liu Lifen

Inventor after: Zhang Tao

Inventor before: Liu Wubiao

Inventor before: Li Youzhi

Inventor before: Pan Deng

Inventor before: Zhang Shuer

Inventor before: Gong Xianzhi

Inventor before: Liu Lifen

Inventor before: Zhang Tao