CN108795768A - A method of being aerated culture microalgae using film - Google Patents

A method of being aerated culture microalgae using film Download PDF

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Publication number
CN108795768A
CN108795768A CN201810354628.XA CN201810354628A CN108795768A CN 108795768 A CN108795768 A CN 108795768A CN 201810354628 A CN201810354628 A CN 201810354628A CN 108795768 A CN108795768 A CN 108795768A
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microalgae
carbon dioxide
method described
culture
hollow
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乔森
田奕晖
金若菲
周集体
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Dalian University of Technology
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Dalian University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae

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  • Biodiversity & Conservation Biology (AREA)
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  • Environmental & Geological Engineering (AREA)
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  • General Engineering & Computer Science (AREA)
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Abstract

The present invention relates to a kind of methods being aerated culture microalgae using film, belong to microdisk electrode technical field.Microalgae of the present invention is chlorella；The culture solution is：Sodium nitrate 1.5g/L, dipotassium hydrogen phosphate 0.04g/L, magnesium sulfate 0.075g/L, calcium chloride 0.036g/L, citric acid 0.006g/L, ferric citrate 0.006g/L, ethylenediamine tetra-acetic acid two receive 0.01g/L, trace element solution 1mL/L, pH 7.0.Present invention optimizes condition of culture, it realizes and reduces carbon dioxide bubble size by using hollow-fibre membrane aeration, while improving utilization rate of carbon dioxide, accelerate the speed of growth of microalgae, to provide a kind of economical and effective new technology for the culture of microalgae and the utilization of carbon dioxide.

Description

A method of being aerated culture microalgae using film
Technical field
The present invention relates to a kind of methods being aerated culture microalgae using film, belong to microdisk electrode technical field.
Background technology
Micro-algae propagation is rapid, can consume a large amount of nutriment, and consuming carbon dioxide by photosynthesis discharges oxygen；Point Cloth is extensive, not tight to environmental condition requirement, adaptable；Also have the effects that decompose, oxidation, filtering, adsorb.As microalgae is trained The development for the technology of supporting, in order to reduce the energy consumption and cost of microdisk electrode, microalgae fixes CO2Technology coupling with sewage disposal becomes Current research hotspot.For CO in fossil fuel combustion waste gas2The problem of excessive emissions, fixes CO about microalgae2Correlation Research is also unfolded from 20 th century laters successively, but absorption of the overwhelming majority research both for microalgae to Carbon Dioxide in Air, The carbon dioxide of only a small number of manually experiment condition proportioning higher concentrations, to carry out research of the microalgae to the resistance to degree of carbon dioxide.
Invention content
Present invention optimizes condition of culture, realize that reduce carbon dioxide bubble by using hollow-fibre membrane aeration big It is small, while improving utilization rate of carbon dioxide, accelerate the speed of growth of microalgae, to be culture and the carbon dioxide of microalgae A kind of economical and effective new technology of offer is provided.
The present invention provides a kind of method being aerated culture microalgae using film, the microalgae is chlorella；
The culture solution is：Sodium nitrate 1.5g/L, dipotassium hydrogen phosphate 0.04g/L, magnesium sulfate 0.075g/L, calcium chloride 0.036g/L, citric acid 0.006g/L, ferric citrate 0.006g/L, ethylenediamine tetra-acetic acid two receive 0.01g/L, and trace element is molten Liquid 1mL/L, pH 7.0；
The trace element solution is：Ethylenediamine tetra-acetic acid 28.14mg/L, zinc sulfate 0.81mg/L, cobalt chloride 0.45mg/ L, manganese chloride 1.86mg/L, copper sulphate 0.48mg/L, sodium molybdate 0.42mg/L, nickel chloride 0.36mg/L, sodium selenate 0.39mg/L, Boric acid 0.027mg/L, sodium tungstate 0.15mg/L.
The present invention is preferably that cultivation temperature is 20-25 DEG C.
The present invention is preferably that intensity of illumination is 8333-25000 lumens.
The present invention is more preferably that intensity of illumination is 8333 lumens, 16666 lumens and 25000 lumens.
The present invention is preferably that density of carbon dioxide gas is 10-40%.
The present invention is more preferably that density of carbon dioxide gas is 10%, 20% and 40%.
The present invention is preferably that hollow-fibre membrane is polyvinylidene fluoride.
The aperture that the present invention is preferably the hollow-fibre membrane is 30-300nm.
The aperture that the present invention is more preferably the hollow-fibre membrane is 30nm, 100nm and 300nm.
The membrane area that the present invention is preferably the hollow-fibre membrane is 10-20cm2。
The present invention is preferably that incubation time is 6 days.
The present invention has the beneficial effect that：
1. the aperture of hollow-fibre membrane of the present invention is decreased to 30nm by 300nm, since carbon dioxide bubble becomes smaller, Carbon dioxide increased resident time in algae solution, utilization rate are increased, micro algae growth is more conducive to；
2. intensity of illumination of the present invention increases to 25000 lumen-hours by 8333 lumens, algae solution can be carried out using illumination Photosynthesis degree increases, and is more conducive to micro algae growth；
3. density of carbon dioxide gas of the present invention is reduced to 10% by 40%, due to algae solution by carbon dioxide compared with The depression effect of high concentration reduces, and is more conducive to micro algae growth.
Description of the drawings
4 width of attached drawing of the present invention,
Fig. 1 is the installation drawing of bottle described in embodiment 1；
Fig. 2 (a), (b), (c), (d), (e), (f), (g), (h) are respectively the algae solution OD that embodiment 1-3 is measured690, pH, algae VSS, chlorophyll a, chlorophyll b, carotenoid, algae filtered fluid TIC, TOC；
Fig. 3 (a), (b), (c), (d), (e), (f), (g), (h) are respectively the algae solution OD that embodiment 3-5 is measured690, pH, algae VSS, chlorophyll a, chlorophyll b, carotenoid, algae filtered fluid TIC, TOC；
Fig. 4 (a), (b), (c), (d), (e), (f), (g), (h) are respectively the algae solution OD that embodiment 5-7 is measured690, pH, algae VSS, chlorophyll a, chlorophyll b, carotenoid, algae filtered fluid TIC, TOC.
Wherein：1, gas-chromatography, 2, experiment bottle, 3, hollow fiber film assembly, 4, water outlet sample tap, 5, rotor, 6, magnetic Power blender, 7, dioxide bottle.
Specific implementation mode
Following non-limiting embodiments can make those skilled in the art be more fully understood the present invention, but not with Any mode limits the present invention.
Test method described in following embodiments is unless otherwise specified conventional method；The reagent and material, such as Without specified otherwise, commercially obtain.
Culture solution is described in following embodiments：Sodium nitrate 1.5g/L, dipotassium hydrogen phosphate 0.04g/L, magnesium sulfate 0.075g/ L, calcium chloride 0.036g/L, citric acid 0.006g/L, ferric citrate 0.006g/L, ethylenediamine tetra-acetic acid two receive 0.01g/L, micro- Secondary element solution 1mL/L；The trace element solution is：Ethylenediamine tetra-acetic acid 28.14mg/L, zinc sulfate 0.81mg/L, chlorination Cobalt 0.45mg/L, manganese chloride 1.86mg/L, copper sulphate 0.48mg/L, sodium molybdate 0.42mg/L, nickel chloride 0.36mg/L, sodium selenate 0.39mg/L, boric acid 0.027mg/L, sodium tungstate 0.15mg/L.
Embodiment 1
A method of it being aerated culture microalgae using film, described method includes following steps：
Algae source：It chooses in constant temperature illumination box and turns 5 days chlorellas of algae, algae 10mL is met per 100mL culture solutions；
Test bottle：Bottle is 1L indigo plant cover glass bottles, and working volume is 660mL (i.e. algae solution 60mL+ culture solution 600mL)；
Bottle reaction condition：It is 25 DEG C to control incubator internal temperature, and intensity of illumination is 8333 lumens, the initial pH of culture solution It is 7.0, hollow-fibre membrane is polyvinylidene fluoride, and the aperture of hollow-fibre membrane is 300nm, and the membrane area of hollow-fibre membrane is 14.57cm2, density of carbon dioxide gas 20%；
The batch experiment method of operation：The every batch of bottle experiment reaction time is 6 days, and daily water sampling measures algae solution OD690、 CO in pH, algae VSS, chlorophyll a, chlorophyll b, carotenoid, TIC, TOC and gas phase2Concentration.
Embodiment 2
A method of it being aerated culture microalgae using film, with being distinguished as embodiment 1：The aperture of hollow-fibre membrane is 100nm。
Embodiment 3
A method of it being aerated culture microalgae using film, with being distinguished as embodiment 1：The aperture of hollow-fibre membrane is 30nm。
Embodiment 4
A method of it being aerated culture microalgae using film, with being distinguished as embodiment 1：The aperture of hollow-fibre membrane is 30nm, intensity of illumination are 16666 lumens.
Embodiment 5
A method of it being aerated culture microalgae using film, with being distinguished as embodiment 1：The aperture of hollow-fibre membrane is 30nm, intensity of illumination are 25000 lumens.
Embodiment 6
A method of it being aerated culture microalgae using film, with being distinguished as embodiment 1：The aperture of hollow-fibre membrane is 30nm, intensity of illumination are 25000 lumens, density of carbon dioxide gas 40%.
Embodiment 7
A method of it being aerated culture microalgae using film, with being distinguished as embodiment 1：The aperture of hollow-fibre membrane is 30nm, intensity of illumination are 25000 lumens, density of carbon dioxide gas 10%.
Algal grown concrete outcome is shown in Table 1 after the completion of above-described embodiment 1-7 batch experiments.
Algal grown concrete outcome after the completion of 1 embodiment 1-7 batch experiments of table
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Embodiment 5 Embodiment 6 Embodiment 7
OD690 0.8801 1.0031 1.9406 2.0658 2.6341 2.0513 2.9776
pH 11.1 11.32 10.05 6.98 7.09 5.29 6.03
Algae VSS 904.57 967.83 1450.05 1358.15 1788.89 1506.96 1983.44
Chlorophyll a 12.91 11.81 18.76 18.33 23.68 13.45 25.36
Chlorophyll b 4.85 3.68 4.84 7.84 8.74 6.04 10.87
Carotenoid 3.18 3.44 5.19 5.36 6.76 4.14 7.99
TIC 3.97 4.52 14.84 35.1 42.49 69.87 68.93
TOC 11.15 13.8 4.46 16.55 18.2 11.09 15.57
CO in gas phase2Concentration 0 0 0 0 17.19 33.37 16.23
It is obtained by table 1：
It is the asynchronous micro algae growth situation of doughnut membrane aperture that embodiment 1-3, which reflects influence factor, experiments have shown that When doughnut membrane aperture is decreased to 30nm by 300nm, since carbon dioxide bubble becomes smaller so that carbon dioxide stops in algae solution The time is stayed to lengthen, utilization rate increases, and is more conducive to micro algae growth；
It is the asynchronous micro algae growth situation of intensity of illumination that embodiment 3-5, which reflects influence factor, experiments have shown that illumination is strong Degree increases to 25000 lumen-hours by 8333 lumens, is more conducive to micro algae growth；
It is the asynchronous micro algae growth situation of density of carbon dioxide gas, experiment card that embodiment 5-7, which reflects influence factor, When bright density of carbon dioxide gas is reduced to 10% by 40%, since algae solution is subtracted by the depression effect of carbon dioxide higher concentration It is small, it is more conducive to micro algae growth.

Claims (8)

1. a kind of method being aerated culture microalgae using film, it is characterised in that：The microalgae is chlorella；
The culture solution is：Sodium nitrate 1.5g/L, dipotassium hydrogen phosphate 0.04g/L, magnesium sulfate 0.075g/L, calcium chloride 0.036g/ L, citric acid 0.006g/L, ferric citrate 0.006g/L, ethylenediamine tetra-acetic acid two receive 0.01g/L, trace element solution 1mL/ L, pH 7.0；
The trace element solution is：Ethylenediamine tetra-acetic acid 28.14mg/L, zinc sulfate 0.81mg/L, cobalt chloride 0.45mg/L, chlorine Change manganese 1.86mg/L, copper sulphate 0.48mg/L, sodium molybdate 0.42mg/L, nickel chloride 0.36mg/L, sodium selenate 0.39mg/L, boric acid 0.027mg/L, sodium tungstate 0.15mg/L.
2. according to the method described in claim 1, it is characterized in that：Cultivation temperature is 20-25 DEG C.
3. according to the method described in claim 2, it is characterized in that：Intensity of illumination is 8333-25000 lumens.
4. according to the method described in claim 3, it is characterized in that：Density of carbon dioxide gas is 10-40%.
5. according to the method described in claim 4, it is characterized in that：Hollow-fibre membrane is polyvinylidene fluoride.
6. according to the method described in claim 5, it is characterized in that：The aperture of the hollow-fibre membrane is 30-300nm.
7. according to the method described in claim 6, it is characterized in that：The membrane area of the hollow-fibre membrane is 10-20cm2。
8. according to the method described in claim 7, it is characterized in that：Incubation time is 6 days.
CN201810354628.XA 2018-04-19 2018-04-19 A method of being aerated culture microalgae using film Pending CN108795768A (en)

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Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101259983A (en) * 2007-12-14 2008-09-10 深圳大学 Method and device for removing nitrogen and phosphorus in immobilized phycomycete coupling membrane separation process
CN102268374A (en) * 2010-05-26 2011-12-07 北京科技大学 Method for culturing microalgae and combined photobioreactor
CN102586221A (en) * 2012-03-08 2012-07-18 大连理工大学 Porous high mass transfer PVA (Polyvinyl Alcohol) embedded carrier and preparation method thereof
CN103435228A (en) * 2013-08-28 2013-12-11 广西大学 Nitrogen and phosphorus removal advanced treatment method for wastewater
CN103468399A (en) * 2013-09-24 2013-12-25 北京化工大学 Method for extracting microbial oil
CN104195198A (en) * 2014-09-01 2014-12-10 石河子大学 Method for extracting polysaccharide from chlorella
CN105062478A (en) * 2015-08-10 2015-11-18 中国科学院新疆理化技术研究所 Biological method for preparing ferrite-bismuth ferrite composite fluorescent material
US20160122705A1 (en) * 2014-11-05 2016-05-05 Palo Alto Research Center Incorporated Dual-compartment bioreactor for use in wastewater treatment and algal production
CN105861370A (en) * 2016-04-25 2016-08-17 江南大学 High-density culture and pre-harvesting method of microalgae
CN106029870A (en) * 2014-01-16 2016-10-12 凯利斯塔公司 Microorganisms for the enhanced production of amino acids and related methods
CN107487859A (en) * 2017-10-11 2017-12-19 广西南宁荣威德新能源科技有限公司 A kind of method of fish-farming with sewage microalgae production livestock fodder additives
CN107904158A (en) * 2017-11-22 2018-04-13 浙江海洋大学 A kind of film aeration microalgae biological film culture device and operation method
CN108767301A (en) * 2018-05-28 2018-11-06 重庆大学 The controllable carbonaceous tubular type oxygen reduction cathode microbiological fuel cell of size and preparation method

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101259983A (en) * 2007-12-14 2008-09-10 深圳大学 Method and device for removing nitrogen and phosphorus in immobilized phycomycete coupling membrane separation process
CN102268374A (en) * 2010-05-26 2011-12-07 北京科技大学 Method for culturing microalgae and combined photobioreactor
CN102586221A (en) * 2012-03-08 2012-07-18 大连理工大学 Porous high mass transfer PVA (Polyvinyl Alcohol) embedded carrier and preparation method thereof
CN103435228A (en) * 2013-08-28 2013-12-11 广西大学 Nitrogen and phosphorus removal advanced treatment method for wastewater
CN103468399A (en) * 2013-09-24 2013-12-25 北京化工大学 Method for extracting microbial oil
CN106029870A (en) * 2014-01-16 2016-10-12 凯利斯塔公司 Microorganisms for the enhanced production of amino acids and related methods
CN104195198A (en) * 2014-09-01 2014-12-10 石河子大学 Method for extracting polysaccharide from chlorella
US20160122705A1 (en) * 2014-11-05 2016-05-05 Palo Alto Research Center Incorporated Dual-compartment bioreactor for use in wastewater treatment and algal production
CN105062478A (en) * 2015-08-10 2015-11-18 中国科学院新疆理化技术研究所 Biological method for preparing ferrite-bismuth ferrite composite fluorescent material
CN105861370A (en) * 2016-04-25 2016-08-17 江南大学 High-density culture and pre-harvesting method of microalgae
CN107487859A (en) * 2017-10-11 2017-12-19 广西南宁荣威德新能源科技有限公司 A kind of method of fish-farming with sewage microalgae production livestock fodder additives
CN107904158A (en) * 2017-11-22 2018-04-13 浙江海洋大学 A kind of film aeration microalgae biological film culture device and operation method
CN108767301A (en) * 2018-05-28 2018-11-06 重庆大学 The controllable carbonaceous tubular type oxygen reduction cathode microbiological fuel cell of size and preparation method

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BRUNO S. FERREIRA等: "Microporous Hollow Fibres for Carbon Dioxide Absorption: Mass Transfer Model Fitting and the Supplying of Carbon Dioxide to Microalgal Cultures", 《JOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY》 *
TAEWOON HWANG等: "Microalgae recovery by ultrafiltration using novel fouling-resistant PVDF membranes with in situ PEGylated polyethyleneimine particles", 《WATER RESEARCH》 *
WEIMING HU等: "Application of nano TiO2 modified hollow fiber membranes in algal membrane bioreactors for high-density algae cultivation and wastewater polishing", 《BIORESOURCE TECHNOLOGY》 *
周集体等: "气升式光生物反应器中Chlorella sp.优化培养与能量计算", 《哈尔滨工业大学学报》 *
田奕晖: "基于膜/光序批式生物反应器培养微藻性能研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 *
赵元元: "膜—光生物反应器处理污水性能及膜污染机理", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 *

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