CN108707545A - A kind of fixed microbial culture garden - Google Patents

A kind of fixed microbial culture garden Download PDF

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Publication number
CN108707545A
CN108707545A CN201810996769.1A CN201810996769A CN108707545A CN 108707545 A CN108707545 A CN 108707545A CN 201810996769 A CN201810996769 A CN 201810996769A CN 108707545 A CN108707545 A CN 108707545A
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CN
China
Prior art keywords
culture
case
feed liquor
guide tube
component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810996769.1A
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Chinese (zh)
Inventor
牟万东
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Jing Qing Green Environment Technology Co Ltd
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Shanghai Jing Qing Green Environment Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Shanghai Jing Qing Green Environment Technology Co Ltd filed Critical Shanghai Jing Qing Green Environment Technology Co Ltd
Priority to CN201810996769.1A priority Critical patent/CN108707545A/en
Publication of CN108707545A publication Critical patent/CN108707545A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a kind of fixed microbial culture gardens, including cultivating outer container, case in incubator body and in the culture of upper and lower ends opening, the upper and lower ends of case and outer side surface form culture solution cyclic gap between the outer chamber interior wall of culture in the culture, central partition plate is installed in case in culture, and the internal cavity of case in culture is divided into half chamber of symmetrical two cultures, one end wall surface of half chamber of culture in central partition plate and corresponding thereto submits fork and is provided with the deflector tilted upward, and cultivating aquaporin of the half intracavitary formation along deflector direction, the bottom end of case is also connected with the feed liquor guide tube that setting is suspended from above culture babinet bottom end in culture, being disposed in feed liquor guide tube makes its internal water be upwardly into the feed liquor component of case and the aeration component above feed liquor component in culture.Compared with prior art, the present invention many-sided can promote to ensure the abundant dissolving mixing of the ingredients such as oxygen in culture solution, effectively improve culture efficiency etc..

Description

A kind of fixed microbial culture garden
Technical field
The invention belongs to field of microbial culture technology, are related to a kind of fixed microbial culture garden.
Background technology
Sewage Biological Treatment is the general name for the method treated sewage for using biology, is most extensive in Modern sewage processing application One of method, dirty Organic substance in water is mainly converted into simple inorganic matter by the decomposition of microorganism, sewage is made to obtain To purification, anaerobic bio-treated and Aerobic biological process two major classes, anaerobic bio-treated system can be divided by oxygen demand situation Organic matter is converted into organic acid using anaerobe, organic acid is decomposed into methane, carbon dioxide and hydrogen etc. by methane backeria again, Such as anaerobic pond, septic tank, the anaerobic digestion of sludge and anaerobic biological reactor, Aerobic biological process system using mechanical aeration or Natural aeration (such as algae photosynthesis produces oxygen) provides the movable energy for aerobic microbiological in sewage, promotes aerobic microbiological Decomposition active makes to purify the sewage, as activated sludge, biofilter, biological rotating disk, sewage irrigation, oxidation pond function.It is dirty Aquatic organism high treating effect, expense is low, and technology is simpler, and application is fairly simple.
Currently, it is common to cultivate the utensil of microorganism in the market, it is general relatively simple, there are various drawbacks, example Such as, cannot effectively into liquid matrix delivery of sufficient amounts gas, be unfavorable for the growth of microorganism；It is unable to constant temperature and ensures microorganism Growing environment；Content poor fluidity, nutriment cannot transmit.
Chinese patent ZL201320225255.9 discloses a kind of microbial cultivation device, including culture babinet, case lid, gas Pump, admission line, bubble generator and outlet pipe are provided with the bubble for extending bubble walking path in incubator body Guide frame.Although the device can extend connecing for bubble and culture solution to a certain extent by the setting of bubbles structure It touches the time and promotes dissolving of the Gaseous nutrition object in culture solution, still, only generated by being aerated component due to water body flow Bubble carries, and internal cyclical effect is very weak, and for microorganism in the culture solution of large volume, gas bubble rate is still It is relatively slow.
Invention content
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of fixed microorganisms Incubator.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of fixed microbial culture garden, including culture outer container, in incubator body and upper and lower ends opening Case in culture forms culture solution cycle in the culture between the upper and lower ends of case and the outer chamber interior wall of the equal and described culture of outer side surface Gap is equipped with central partition plate in case in the culture, and the internal cavity of case in the culture is divided into symmetrical two Half chamber of a culture, an end wall surface of half chamber of culture in central partition plate and corresponding thereto submit fork and are provided with the water conservancy diversion tilted upward Plate, and the bottom end of case is also connected with setting along the aquaporin in the deflector direction, the culture in the formation of half intracavitary of culture The feed liquor guide tube being suspended from above culture babinet bottom end, being disposed in feed liquor guide tube makes its internal water be upwardly into case in culture Feed liquor component and the aeration component above feed liquor component.
Further, the feed liquor component includes the spiral that liquid stream of boosting is rotated and formed in feed liquor guide tube Paddle.
Further, the bottom of case is also connected with and goes out liquid guide tube less than the culture outer container top in the culture, Go out also be also equipped in liquid guide tube so that culture solution is flowed outwardly go out liquid component.It may be to be set as feed liquor component to go out liquid component It is set to propeller form, setting can utilize the water suction effect that top generates, and promote case and the training in culture outer container in culture Nutrient solution circulates.
Further, along deflector inclined direction, the upper end of the deflector and central partition plate or culture internal box wall face it Between be additionally provided with the porous plate for being densely covered with micropore.
Further, the aperture of micropore is less than the bubble aperture that aeration component generates on porous plate.
Further, it is additionally provided with outlet pipe at the top of the culture outer container.
Further, the lateral wall of the described culture outer container is equipped with heat preservation sandwich layer, be disposed in the heat preservation sandwich layer by Controller controls the electrical heating elements of work, and the temperature that controller described in feedback link is additionally provided on cultivating outer chamber interior wall passes Sensor.
Compared with prior art, the present invention has the following advantages：
(1) present invention, by feed liquor component, is aerated component and goes out liquid group by the bottom portion setting feed liquor guide tube in culture Part coordinates so that be more readily formed circulating fluid in case and culture outer container in culture, quickening promote the Gaseous nutritions such as oxygen at Abundant dissolving of the grouping point in culture solution.
(2) by the way that porous plate is arranged in the upper end of deflector, and ensure that micropore size is less than aeration component production on porous plate Raw bubble aperture so that bubble can be drawn as more tiny microbubble, further after with circulating water flow by porous plate Dissolution time of the gas in culture solution is extended, in addition, " cavitation erosion " effect that culture solution is generated by micropore also promotes base The turbulating effect of matter ensure that being uniformly mixed for nutritional ingredient in culture solution.
(3) by electrical heating elements in heat preservation sandwich layer and temperature sensor etc., the temperature of culture solution can be accurately controlled Degree is in optimum range.
Description of the drawings
Fig. 1 is the structural diagram of the present invention；
Description of symbols in figure：
1- cultivates outer container, the interior case of 2- cultures, 3- culture solution cyclic gaps, 4- central partition plates, 5- temperature sensors, 6- aerations Component, 7- feed liquor components, 8- feed liquor guide tubes, 9- half chambers of culture, 10- porous plates, 11- deflectors, 12- heat preservation sandwich layers, 13- electricity add Thermal element, 14- go out liquid guide tube, and 15- goes out liquid component, 16- outlet pipes, 17- permeable holes.
Specific implementation mode
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.The present embodiment is with technical solution of the present invention Premised on implemented, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to Following embodiments.
In following embodiment, existing conventional aeration tube etc. may be used in aeration component.
Embodiment 1
A kind of fixed microbial culture garden, it is shown in Figure 1, including culture outer container 1, be mounted on incubator body in and on Case 2 in the culture of lower both ends open, in the culture upper and lower ends of case 2 with outer side surface and 1 inner wall of culture outer container it Between form culture solution cyclic gap 3, central partition plate 4 is installed in case 2 in the culture, and will be in case 2 in the culture Portion's cavity is divided into half chamber 9 of symmetrical two cultures, in an end wall surface of half chamber 9 of culture in central partition plate 4 and corresponding thereto It is arranged in a crossed manner to have the deflector 11 tilted upward, and form the aquaporin along 11 direction of the deflector, institute in half chamber 9 of culture The bottom end of case 2 is also connected with the feed liquor guide tube 8 that setting is suspended from above culture babinet bottom end, the cloth in feed liquor guide tube 8 in the culture stated Being equipped with makes its internal water be upwardly into the feed liquor component 7 of case 2 and the aeration component 6 above feed liquor component 7 in culture.
Wherein, the feed liquor component 7 includes the propeller that liquid stream of boosting is rotated and formed in feed liquor guide tube 8, The bottom of case 2 is also connected in the culture goes out liquid guide tube 14 less than 1 top of the culture outer container, is going out in liquid guide tube 14 Be also equipped with so that culture solution is flowed outwardly go out liquid component 15, go out liquid component 15 or to be arranged to spiral shell as feed liquor component 7 Paddle form is revolved, setting can utilize the water suction effect that top generates, and promote case 2 and the culture solution in culture outer container 1 in culture Circulate.
In addition, in order to further promote mixing well for abundant dissolving and nutritional ingredient of the bubble in culture solution, edge is led Be additionally provided between case 2 wall surface in 11 inclined direction of flowing plate, the upper end of the deflector 11 and central partition plate 4 or culture be densely covered with it is micro- The porous plate 10 in hole, the aperture of micropore is less than the bubble aperture that aeration component 6 generates on porous plate 10.The culture outer container 1 Top be additionally provided with outlet pipe 16.
Embodiment 2
On the basis of embodiment 1, the present embodiment further uses following design：
Referring again to Fig. 1, the lateral wall of the described culture outer container 1 is equipped with heat preservation sandwich layer 12, in the heat preservation sandwich layer 12 Be disposed with by controller control work electrical heating elements 13 (electrical heating elements 13 may be used commonly used in the art such as electrical heating The heating elements such as silk), the temperature sensor 5 of controller described in feedback link is additionally provided on culture 1 inner wall of outer container.
Specific heating process is, the temperature signal of 5 Real-time Feedback culture solution of temperature sensor is to controller, and by controller Electrical heating elements 13 are run, and the temperature in culture solution is made to be maintained within the scope of set temperature.
Embodiment 3
On the basis of embodiment 1 or embodiment 2, the present embodiment further uses following design：
Referring again to Fig. 1, two permeable holes 17 for being connected of half chambers 9 of culture are also arranged such that on central partition plate 4, it can be with Culture solution in cultivating inside case 2 is mixed more uniform.
The above description of the embodiments is intended to facilitate ordinary skill in the art to understand and use the invention. Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein general Principle is applied in other embodiment without having to go through creative labor.Therefore, the present invention is not limited to the above embodiments, ability Field technique personnel announcement according to the present invention, improvement and modification made without departing from the scope of the present invention all should be the present invention's Within protection domain.

Claims (7)

1. a kind of fixed microbial culture garden, including culture outer container, in incubator body and the training of upper and lower ends opening Case in supporting is formed between culture solution cycle between the upper and lower ends of case and the outer chamber interior wall of the equal and described culture of outer side surface in the culture Gap is equipped with central partition plate in case in the culture, and the internal cavity of case in the culture is divided into symmetrical two Half chamber is cultivated, an end wall surface of half chamber of culture in central partition plate and corresponding thereto submits fork and is provided with the water conservancy diversion tilted upward Plate, and cultivating aquaporin of the half intracavitary formation along the deflector direction, which is characterized in that the bottom end of case in the culture It is also connected with the feed liquor guide tube that setting is suspended from above culture babinet bottom end, being disposed in feed liquor guide tube makes its internal water be upwardly into The feed liquor component of case and the aeration component above feed liquor component in culture.
2. a kind of fixed microbial culture garden according to claim 1, which is characterized in that the feed liquor component includes The propeller for liquid stream of boosting is rotated and formed in feed liquor guide tube.
3. a kind of fixed microbial culture garden according to claim 1, which is characterized in that the bottom of case in the culture Portion is also connected with goes out liquid guide tube less than the culture outer container top, so that culture solution is flowed outwardly going out also to be also equipped in liquid guide tube Go out liquid component.
4. a kind of fixed microbial culture garden according to claim 1, which is characterized in that along deflector inclined direction, It is additionally provided with the porous plate for being densely covered with micropore between the upper end of the deflector and central partition plate or culture internal box wall face.
5. a kind of fixed microbial culture garden according to claim 4, which is characterized in that the aperture of micropore on porous plate The bubble aperture generated less than aeration component.
6. a kind of fixed microbial culture garden according to claim 1, which is characterized in that the top of the culture outer container Portion is additionally provided with outlet pipe.
7. a kind of fixed microbial culture garden according to claim 1, which is characterized in that outside the culture outer container Side wall is equipped with heat preservation sandwich layer, and the electrical heating elements that work is controlled by controller are disposed in the heat preservation sandwich layer, outer in culture The temperature sensor of controller described in feedback link is additionally provided on chamber interior wall.
CN201810996769.1A 2018-08-29 2018-08-29 A kind of fixed microbial culture garden Pending CN108707545A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810996769.1A CN108707545A (en) 2018-08-29 2018-08-29 A kind of fixed microbial culture garden

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810996769.1A CN108707545A (en) 2018-08-29 2018-08-29 A kind of fixed microbial culture garden

Publications (1)

Publication Number Publication Date
CN108707545A true CN108707545A (en) 2018-10-26

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112226347A (en) * 2020-10-20 2021-01-15 中南大学 Microorganism continuous culture device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112226347A (en) * 2020-10-20 2021-01-15 中南大学 Microorganism continuous culture device

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