CN107485891A - 改良的层析装置及其用于连续流层析的方法 - Google Patents
改良的层析装置及其用于连续流层析的方法 Download PDFInfo
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- B01D15/08—Selective adsorption, e.g. chromatography
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110348090A (zh) * | 2019-06-28 | 2019-10-18 | 浙江大学 | 基于人工神经网络实现多柱连续流层析设计及分析的方法 |
CN110348089A (zh) * | 2019-06-28 | 2019-10-18 | 浙江大学 | 基于层析模型实现多柱连续流层析设计及分析的方法 |
CN111068360A (zh) * | 2018-10-22 | 2020-04-28 | 信达生物制药(苏州)有限公司 | 组合式层析装置和连续流层析方法 |
WO2020258706A1 (zh) * | 2019-06-28 | 2020-12-30 | 信达生物制药(苏州)有限公司 | 组合式层析装置、无缝连接层析方法和生物制剂的纯化方法 |
WO2020258705A1 (zh) * | 2019-06-28 | 2020-12-30 | 信达生物制药(苏州)有限公司 | 模块化层析装置 |
CN112451996A (zh) * | 2020-11-10 | 2021-03-09 | 浙江大学 | 一种多柱连续流层析捕获蛋白的优化方法 |
CN114026108A (zh) * | 2019-06-28 | 2022-02-08 | 信达生物制药(苏州)有限公司 | 无缝连续流层析方法 |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4935040A (en) * | 1989-03-29 | 1990-06-19 | The Perkin-Elmer Corporation | Miniature devices useful for gas chromatography |
US20020116137A1 (en) * | 2000-05-08 | 2002-08-22 | Kirts Richard Eugene | Method for detecting and measuring foam forming compounds in aqueous solutions |
JP2003156482A (ja) * | 2001-11-19 | 2003-05-30 | Takenori Tanimura | 調製クロマトグラフィ装置およびそれを用いた分離・精製方法 |
CN102257134A (zh) * | 2008-02-12 | 2011-11-23 | 赛诺菲巴斯德有限公司 | 使用离子交换和凝胶过滤色谱进行痘病毒纯化的方法 |
CN104458953A (zh) * | 2014-12-05 | 2015-03-25 | 北京佰纯润宇生物科技有限公司 | 基于三位七通阀实现层析柱正反流及旁路功能的层析系统 |
CN105107230A (zh) * | 2015-09-09 | 2015-12-02 | 南月英 | 一种层析仪改装用于灌流培养纯化的方法 |
CN105203675A (zh) * | 2015-08-18 | 2015-12-30 | 苏州赛谱仪器有限公司 | 连续进样层析装置 |
CN106029119A (zh) * | 2014-02-19 | 2016-10-12 | 美敦力公司 | 用于在线再装填吸附剂材料的模块与任选的旁路 |
-
2017
- 2017-07-20 CN CN201710594337.3A patent/CN107485891B/zh active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4935040A (en) * | 1989-03-29 | 1990-06-19 | The Perkin-Elmer Corporation | Miniature devices useful for gas chromatography |
US20020116137A1 (en) * | 2000-05-08 | 2002-08-22 | Kirts Richard Eugene | Method for detecting and measuring foam forming compounds in aqueous solutions |
JP2003156482A (ja) * | 2001-11-19 | 2003-05-30 | Takenori Tanimura | 調製クロマトグラフィ装置およびそれを用いた分離・精製方法 |
CN102257134A (zh) * | 2008-02-12 | 2011-11-23 | 赛诺菲巴斯德有限公司 | 使用离子交换和凝胶过滤色谱进行痘病毒纯化的方法 |
CN106029119A (zh) * | 2014-02-19 | 2016-10-12 | 美敦力公司 | 用于在线再装填吸附剂材料的模块与任选的旁路 |
CN104458953A (zh) * | 2014-12-05 | 2015-03-25 | 北京佰纯润宇生物科技有限公司 | 基于三位七通阀实现层析柱正反流及旁路功能的层析系统 |
CN105203675A (zh) * | 2015-08-18 | 2015-12-30 | 苏州赛谱仪器有限公司 | 连续进样层析装置 |
CN105107230A (zh) * | 2015-09-09 | 2015-12-02 | 南月英 | 一种层析仪改装用于灌流培养纯化的方法 |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111068360A (zh) * | 2018-10-22 | 2020-04-28 | 信达生物制药(苏州)有限公司 | 组合式层析装置和连续流层析方法 |
CN110348090A (zh) * | 2019-06-28 | 2019-10-18 | 浙江大学 | 基于人工神经网络实现多柱连续流层析设计及分析的方法 |
CN110348089A (zh) * | 2019-06-28 | 2019-10-18 | 浙江大学 | 基于层析模型实现多柱连续流层析设计及分析的方法 |
WO2020258706A1 (zh) * | 2019-06-28 | 2020-12-30 | 信达生物制药(苏州)有限公司 | 组合式层析装置、无缝连接层析方法和生物制剂的纯化方法 |
WO2020258705A1 (zh) * | 2019-06-28 | 2020-12-30 | 信达生物制药(苏州)有限公司 | 模块化层析装置 |
CN110348090B (zh) * | 2019-06-28 | 2021-05-04 | 浙江大学 | 基于人工神经网络实现多柱连续流层析设计及分析的方法 |
CN114026108A (zh) * | 2019-06-28 | 2022-02-08 | 信达生物制药(苏州)有限公司 | 无缝连续流层析方法 |
CN114072217A (zh) * | 2019-06-28 | 2022-02-18 | 信达生物制药(苏州)有限公司 | 组合式层析装置、无缝连接层析方法和生物制剂的纯化方法 |
CN112451996A (zh) * | 2020-11-10 | 2021-03-09 | 浙江大学 | 一种多柱连续流层析捕获蛋白的优化方法 |
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Address after: Room 701, 7th Floor, Huajing Road, Waigaoqiao Free Trade Zone, Pudong New Area, Shanghai, 2001 Applicant after: SHANGHAI YAOMING BIOTECHNOLOGY CO., LTD. Applicant after: Wuxi Yaoming Biotechnology Co., Ltd. Applicant after: SUZHOU YAOMING KANGDE INSPECTION TESTING CO., LTD. Address before: Room 701, 7th Floor, Huajing Road, Waigaoqiao Free Trade Zone, Pudong New Area, Shanghai, 2001 Applicant before: SHANGHAI YAOMING BIOTECHNOLOGY CO., LTD. Applicant before: WUXI APPTEC BIOPHARMACEUTICALS CO., LTD. Applicant before: SUZHOU YAOMING KANGDE INSPECTION TESTING CO., LTD. |
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Address after: Room 701, 7th Floor, Huajing Road, Waigaoqiao Free Trade Zone, Pudong New Area, Shanghai, 2001 Applicant after: SHANGHAI YAOMING BIOTECHNOLOGY CO., LTD. Applicant after: Wuxi Yaoming Biotechnology Co., Ltd. Applicant after: Suzhou Pharmacopoeia Testing and Inspection Co., Ltd. Address before: Room 701, 7th Floor, Huajing Road, Waigaoqiao Free Trade Zone, Pudong New Area, Shanghai, 2001 Applicant before: SHANGHAI YAOMING BIOTECHNOLOGY CO., LTD. Applicant before: Wuxi Yaoming Biotechnology Co., Ltd. Applicant before: SUZHOU YAOMING KANGDE INSPECTION TESTING CO., LTD. |
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Effective date of registration: 20211213 Address after: 215104 North Side of Wuzhong Avenue, Wuzhong Economic Development Zone, Suzhou City, Jiangsu Province Patentee after: Suzhou Pharmacopoeia Testing and Inspection Co.,Ltd. Address before: Room 701, 7th Floor, Huajing Road, Waigaoqiao Free Trade Zone, Pudong New Area, Shanghai, 2001 Patentee before: WUXI BIOLOGICS (SHANGHAI) Co.,Ltd. Patentee before: Wuxi Yaoming Biotechnology Co.,Ltd. Patentee before: Suzhou Pharmacopoeia Testing and Inspection Co.,Ltd. |