CN107412336A - Fruit of Chinese magnoliavine lignanoid and preparation method and application with antioxidant and anti-aging activity - Google Patents
Fruit of Chinese magnoliavine lignanoid and preparation method and application with antioxidant and anti-aging activity Download PDFInfo
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- CN107412336A CN107412336A CN201710733047.2A CN201710733047A CN107412336A CN 107412336 A CN107412336 A CN 107412336A CN 201710733047 A CN201710733047 A CN 201710733047A CN 107412336 A CN107412336 A CN 107412336A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses a kind of fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity and its preparation method and application, comprise the steps of：(1) by fruit of Chinese magnoliavine residue dries pulverizing, fruit of Chinese magnoliavine residue powder is obtained；(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight is added, 1~2% skimmed milk power, 0.1~1% sucrose, is mixed；Ultraviolet disinfection 5min；(3) Bifidobacteria powder is added after sterilizing, mixes and cultivates 3d, after fermentation ends, solid constituent is taken out and dried, must ferment fruit of Chinese magnoliavine residue powder；(4) will ferment fruit of Chinese magnoliavine residue powder, 70~80% ethyl acetate immersion 20min of 8 times of parts by weight be added, through supercritical CO2Extraction, obtains fruit of Chinese magnoliavine residue extract；(5) fruit of Chinese magnoliavine residue extract is isolated and purified, be concentrated under reduced pressure, vacuum drying.The present invention is capable of the activity of anti-lipid peroxidation, additionally it is possible to removes free radical, effectively plays antioxidation, the effect of so as to play anti-aging.
Description
Technical field
The present invention relates to cosmetic field, and in particular to it is a kind of with antioxidant and anti-aging activity fruit of Chinese magnoliavine lignanoid and
Preparation method and application.
Background technology
The fruit of Chinese magnoliavine, alias taste, medicine grape, Chinese prickly ash, it is Magnoliaceae Schizandra liana.Schizandra lignanoid
Class compound is with the lignanoid that 1,2,3,4- biphenyl -1,3- cyclo-octadiene is parent nucleus, is that most important pharmacology is lived in the fruit of Chinese magnoliavine
Property composition；Domestic and foreign scholars lignanoids monomeric compound isolated from Schizandra has kind more than 150, wherein having
The monomer for having obvious biological activity includes：Schizandrin A (Deoxyschisandrin), deoxyschizandrin (Schisandrin
B), schisandrin C (Schisan-drin C), schizandrin (Schisandrol A), wuweizi alcohol B
(Schisandrol B), Schisantherin C (Schisantherin A), wuweizi ester B (Schisantherin B), dagger-axe rice
Pungent A (Gomisin A) etc..Leather phoenix etc. have studied fruit of Chinese magnoliavine lignanoid to rat liver, the suppression of cerebrum lipid peroxidating
Activity and the scavenging action to free radical, the results showed that, schizandrin A, schizandrin, wuweizi alcohol B are main resist
Oxidative active ingredients.The deoxyschizandrin that is extracted from the fruit of Chinese magnoliavine, fruit of Chinese magnoliavine glycol, Schisandrone are to vitamin C-NADPH
System or half skins of FeSO4--brain of propylhomoserin system induction, liver, kidney foundation of microsomal Lipid Peroxidation have obvious inhibiting effect, to O2-It is clear
Except one of mechanism for being these composition anti-lipid peroxidations.
The content of the invention
Based on this, it is an object of the invention to provide a kind of fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity and system
Preparation Method and application.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, is comprised the steps of：
(1) by fruit of Chinese magnoliavine residue, after 45 DEG C dry and crush, fruit of Chinese magnoliavine residue powder is obtained；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight of addition, 1~2% skimmed milk power, 0.1~1% sugarcane
Sugar, mix；Ultraviolet irradiation sterilization 5min；
(3) Bifidobacteria powder is added after sterilizing, is mixed, is placed under 32 DEG C of constant temperature ventilation conditions and cultivates 3d, fermentation ends
Afterwards, solid constituent is taken out and dried, must ferment fruit of Chinese magnoliavine residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add 70~80% ethyl acetate immersion 20min of 8 times of parts by weight, pass through
After SCF-CO 2, fruit of Chinese magnoliavine residue extract is obtained；
(5) by fruit of Chinese magnoliavine residue extract, purifying is isolated, is concentrated under reduced pressure, be dried in vacuo, obtain fruit of Chinese magnoliavine lignanoid.
In wherein some embodiments, the fruit of Chinese magnoliavine residue is fruit of Chinese magnoliavine seed, pulp, rattan, leaf, root by water extraction
Remaining residue afterwards.
In wherein some embodiments, the fruit of Chinese magnoliavine residue powder in the step (1) needed 50~70 mesh sieves.
In wherein some embodiments, the SCF-CO 2 parameter in the step (4) is：36~43 DEG C of constant temperature extractions
Take, extracting pressure 23Mpa, 33 DEG C of extraction temperature, extraction time 1.5h, CO2 flow 5L/min.
In wherein some embodiments, the method that fruit of Chinese magnoliavine residue is isolated and purified in the step (5) is using following ginseng
Several macroreticular resin absorbing methods：The applied sample amount of 4~5 times of bed volumes, elution flow rate 1m L/min, distilled water elution volume are 5
~7 times of bed volumes, eluant, eluent are 85~95% ethanol, and eluting agent is 5 times of bed volumes.
Present invention also offers the fruit of Chinese magnoliavine wood with antioxidant and anti-aging activity according to obtained by above-mentioned preparation method
Fat element.
Present invention also offers the above-mentioned fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity in cosmetics are prepared
Using.
In wherein some embodiments, the State of cosmetics includes cream, emulsion, aqua.
Present invention also offers a kind of cosmetics, including the foregoing fruit of Chinese magnoliavine wood with antioxidant and anti-aging activity
Fat element.
Present invention also offers a kind of facial mask, includes following mass percent composition：Have as previously described anti-oxidant anti-
The fruit of Chinese magnoliavine lignanoid 0.2~2% of aging activity, glycerine 3~8%, butanediol 3~8%, propane diols 3~8%, ethoxy urea 1
~6%, niacinamide 1~5%, Sodium Hyaluronate 0.02~0.06%, Phenoxyethanol 0.3~0.8%, methyl hydroxybenzoate 0.1~
0.3%th, Cremophor RH40 0.01~0.5%, methylisothiazolinone 0.002~0.008%, iodine propilolic alcohol butyl ammonia
Formic acid esters 0.01~0.03%, deionized water surplus.
It is the device have the advantages that as follows：The present invention, which is largely tested by inventor, and experience is preferred prepares
The various conditions of method, the preparation method optimized, the fruit of Chinese magnoliavine lignanoid obtained using preparation method of the present invention, contain
Amount is high, and properties of product are stable, can add in a variety of skin-protection products, such as cream, emulsion, aqua.The present invention can suppress fat
The activity of matter peroxidating, additionally it is possible to remove free radical, effectively play antioxidation, the effect of so as to play anti-aging.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated：
All the components appeared in text are INCI (International Nomenclature Cosmetic
Ingredient), i.e., international cosmetic raw material is obviously said to its defined title.
Embodiment 1
A kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, is comprised the steps of：
(1) by remaining residue after fruit of Chinese magnoliavine water extraction, dried by 45 DEG C and crushed 60 mesh sieves, obtain fruit of Chinese magnoliavine residue
Powder；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight is added, 1.5% skimmed milk power, 0.5% sucrose, is mixed
It is even；Ultraviolet irradiation sterilization 5min；
(3) Bifidobacteria powder is added after sterilizing, is mixed, is placed under 32 DEG C of constant temperature ventilation conditions and cultivates 3d, fermentation ends
Afterwards, solid constituent is taken out and dried, must ferment fruit of Chinese magnoliavine residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add the 75% ethyl acetate immersion 20min of 8 times of parts by weight, join in extraction
Number is：40 DEG C of constant temperature extractings, extracting pressure 23Mpa, 33 DEG C of extraction temperature, extraction time 1.5h, CO2 flow 5L/min condition
Under, after SCF-CO 2, obtain fruit of Chinese magnoliavine residue extract；
(5) it is 6 times of post bed bodies in the applied sample amount of 4.5 times of bed volumes, elution flow rate 1mL/min, distilled water elution volume
Product, eluant, eluent is 90% ethanol, under conditions of eluting agent is 5 times of bed volume, fruit of Chinese magnoliavine residue extract is separated pure
Change, be concentrated under reduced pressure, be dried in vacuo, obtain fruit of Chinese magnoliavine lignanoid.
Embodiment 2
A kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, is comprised the steps of：
(1) by remaining residue after fruit of Chinese magnoliavine water extraction, dried by 45 DEG C and crushed 50 mesh sieves, obtain fruit of Chinese magnoliavine residue
Powder；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight is added, 1% skimmed milk power, 0.1% sucrose, is mixed
It is even；Ultraviolet irradiation sterilization 5min；
(3) Bifidobacteria powder is added after sterilizing, is mixed, is placed under 32 DEG C of constant temperature ventilation conditions and cultivates 3d, fermentation ends
Afterwards, solid constituent is taken out and dried, must ferment fruit of Chinese magnoliavine residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add the 70% ethyl acetate immersion 20min of 8 times of parts by weight, join in extraction
Number is：36 DEG C of constant temperature extractings, extracting pressure 23Mpa, 33 DEG C of extraction temperature, extraction time 1.5h, CO2 flow 5L/min condition
Under, after SCF-CO 2, obtain fruit of Chinese magnoliavine residue extract；
(5) it is 5 times of bed volumes in the applied sample amount of 4 times of bed volumes, elution flow rate 1mL/min, distilled water elution volume,
Eluant, eluent is 85% ethanol, and under conditions of eluting agent is 5 times of bed volume, fruit of Chinese magnoliavine residue extract is isolated and purified,
It is concentrated under reduced pressure, is dried in vacuo, obtains fruit of Chinese magnoliavine lignanoid.
Embodiment 3
A kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, is comprised the steps of：
(1) by remaining residue after fruit of Chinese magnoliavine water extraction, dried by 45 DEG C and crushed 70 mesh sieves, obtain fruit of Chinese magnoliavine residue
Powder；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight is added, 2% skimmed milk power, 1% sucrose, is mixed；
Ultraviolet irradiation sterilization 5min；
(3) Bifidobacteria powder is added after sterilizing, is mixed, is placed under 32 DEG C of constant temperature ventilation conditions and cultivates 3d, fermentation ends
Afterwards, solid constituent is taken out and dried, must ferment fruit of Chinese magnoliavine residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add the 80% ethyl acetate immersion 20min of 8 times of parts by weight, join in extraction
Number is：43 DEG C of constant temperature extractings, extracting pressure 23Mpa, 33 DEG C of extraction temperature, extraction time 1.5h, CO2 flow 5L/min condition
Under, after SCF-CO 2, obtain fruit of Chinese magnoliavine residue extract；
(5) it is 7 times of bed volumes in the applied sample amount of 5 times of bed volumes, elution flow rate 1mL/min, distilled water elution volume,
Eluant, eluent is 95% ethanol, and under conditions of eluting agent is 5 times of bed volume, fruit of Chinese magnoliavine residue extract is isolated and purified,
It is concentrated under reduced pressure, is dried in vacuo, obtains fruit of Chinese magnoliavine lignanoid.
Comparative example 1
A kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, is comprised the steps of：
(1) by remaining residue after fruit of Chinese magnoliavine water extraction, dried by 50 DEG C and crushed 80 mesh sieves, obtain fruit of Chinese magnoliavine residue
Powder；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight is added, 2% skimmed milk power, 1.5% sucrose, is mixed
It is even；Ultraviolet irradiation sterilization 10min；
(3) Bifidobacteria powder is added after sterilizing, is mixed, is placed under 35 DEG C of constant temperature ventilation conditions and cultivates 3d, fermentation ends
Afterwards, solid constituent is taken out and dried, must ferment fruit of Chinese magnoliavine residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add the 90% ethyl acetate immersion 25min of 10 times of parts by weight, extract
Parameter is：50 DEG C of constant temperature extractings, extracting pressure 25Mpa, 35 DEG C of extraction temperature, extraction time 2h, CO2Flow 5L/min condition
Under, by supercritical CO2After extraction, fruit of Chinese magnoliavine residue extract is obtained；
(5) it is 7 times of post bed bodies in the applied sample amount of 6 times of bed volumes, elution flow rate 1.5mL/min, distilled water elution volume
Product, eluant, eluent is 70% ethanol, under conditions of eluting agent is 5 times of bed volume, fruit of Chinese magnoliavine residue extract is separated pure
Change, be concentrated under reduced pressure, be dried in vacuo, obtain fruit of Chinese magnoliavine lignanoid.
Comparative example 2
A kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, is comprised the steps of：
(1) by remaining residue after fruit of Chinese magnoliavine water extraction, dried by 45 DEG C and crushed 60 mesh sieves, obtain fruit of Chinese magnoliavine residue
Powder；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight is added, 1.5% skimmed milk power, 0.5% sucrose, is mixed
It is even；Ultraviolet irradiation sterilization 5min；
(3) it is placed in after sterilizing under 32 DEG C of constant temperature ventilation conditions after cultivating 3d, solid constituent is taken out and dried, obtains the fermentation five tastes
Sub- residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add the 75% ethyl acetate immersion 20min of 8 times of parts by weight, join in extraction
Number is：36~43 DEG C of constant temperature extractings, extracting pressure 23Mpa, 33 DEG C of extraction temperature, extraction time 1.5h, CO2 flow 5L/min's
Under the conditions of, after SCF-CO 2, obtain fruit of Chinese magnoliavine residue extract；
(5) it is 6 times of post bed bodies in the applied sample amount of 4.5 times of bed volumes, elution flow rate 1mL/min, distilled water elution volume
Product, eluant, eluent is 90% ethanol, under conditions of eluting agent is 5 times of bed volume, fruit of Chinese magnoliavine residue extract is separated pure
Change, be concentrated under reduced pressure, be dried in vacuo, obtain fruit of Chinese magnoliavine lignanoid.
Comparative example 3
A kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, is comprised the steps of：
(1) by remaining residue after fruit of Chinese magnoliavine water extraction, dried by 45 DEG C and crushed 60 mesh sieves, obtain fruit of Chinese magnoliavine residue
Powder；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight of addition, ultraviolet irradiation sterilization 5min；
(3) Bifidobacteria powder is added after sterilizing, is mixed, is placed under 35 DEG C of constant temperature ventilation conditions and cultivates 3d, fermentation ends
Afterwards, solid constituent is taken out and dried, must ferment fruit of Chinese magnoliavine residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add the 75% ethyl acetate immersion 20min of 10 times of parts by weight, extract
Parameter is：50 DEG C of constant temperature extractings, extracting pressure 30Mpa, 35 DEG C of extraction temperature, extraction time 1.5h, CO2 flow 5L/min bar
Under part, after SCF-CO 2, fruit of Chinese magnoliavine residue extract is obtained；
(5) it is 6 times of bed volumes in the applied sample amount of 5 times of bed volumes, elution flow rate 1mL/min, distilled water elution volume,
Eluant, eluent is 90% ethanol, and under conditions of eluting agent is 5 times of bed volume, fruit of Chinese magnoliavine residue extract is isolated and purified,
It is concentrated under reduced pressure, is dried in vacuo, obtains fruit of Chinese magnoliavine lignanoid.
Interpretation of result：The experiment of the present invention is with reference to Song Gui pretty young woman's in 2003《The medicinal study of fructus schisandrae carpopodium》The inside
Assay method, the content for preparing fruit of Chinese magnoliavine lignanoid is measured at 570nm wavelength with discoloration acid colorimetric method.By embodiment 1-3 and
The fruit of Chinese magnoliavine lignanoid content record such as table 1 below that comparative example 1-3 preparation condition is prepared.
Table 1
From the data analysis of table 1：The content of the fruit of Chinese magnoliavine lignanoid obtained under embodiment 1-3 preparation condition substantially compares
The content of the fruit of Chinese magnoliavine lignanoid obtained under comparative example 1-3 preparation condition is substantially higher；Comparative example 1 changes preparation technology's
All parameters, the content for the fruit of Chinese magnoliavine lignanoid that Comparative result example 1 obtains are significantly lower than embodiment 1；The preparation technology of comparative example 2
In without adding skimmed milk power and sucrose, but extracted amount is substantially lower than embodiment 1, illustrates the skimmed milk power and sucrose of preparation technology
Fermentation process can be influenceed, and then influences recovery rate, therefore, it is necessary to controls the nutrient environment in preparation technology；Comparative example 3 prepares work
Skill does not add Bifidobacteria powder, and residue itself carries out fermentation process, but recovery rate is also very low, illustrates to add bifid bar
Bacterium bacterium powder, the fermentation process of residue is promoted, effectively improves recovery rate.
The fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity that embodiment 1 is prepared is applied in facial mask, is had
Body such as table 2 below.
Table 2
Anti-oxidation efficacy is verified
Compound concentration is 1 × 10-2Mol/L DPPH ethanol solutions.The fruit of Chinese magnoliavine wood fat that embodiment 1 is obtained
Facial mask liquid in element, and above-mentioned application, it is respectively 0.01,0.10,0.15,0.20,0.25 to be configured to concentration with 80% ethanol
Testing sample solution.5mL sample solutions and DPPH solution are taken respectively, and are well mixed, avoid light place 30min, with 80% second
Alcohol is returned to zero, and absorbance A i is determined at 517BITI；Same method, take 5mL80% alcohol solvents mutually mixed with sample solution respectively
Close uniform, measure absorbance A j after avoid light place 30min；5mL80% alcohol solvents add 5mLDPPH solution to mix, and lucifuge is put
Put 30min, measure absorbance A c.Finally free radical scavenging activity is calculated according to below equation：IP%=[1- (Ai-Aj)/Ac] ×
100, Ai is sample and the absorption values after DPPH effects in formula；Aj is contribution of the sample itself to absorbance；Ac is
DPPH itself absorption.The removing DPPH of facial mask liquid in the fruit of Chinese magnoliavine lignanoid that embodiment 1 is prepared and application
Ability measurement result see the table below 3.
Table 3
From the data of upper table 3, the oxidation resistance of fruit of Chinese magnoliavine lignanoid increases with the increase by two of concentration, and antioxygen
Change ability is strong；Applied in mask product, impart the good oxidation resistance of product, skin can be made more pale, light
It is sliding, it is flexible.
Senile-resistant efficacy is verified
Healthy volunteer 80 is chosen, is divided into two groups, 30~65 years old age；There are certain Dermatochalasia/fine wrinkles existing
As, and experiment is voluntarily completed on request；The facial mask of above-mentioned preparation is test group, and the facial mask for laying claim to anti-aging effects on the market is
Control group；Test period is one month, and when using the 30th day, product effect is evaluated using the method for trier's self-appraisal.As a result
Such as table 4 below.
Table 4
As can be seen from Table 4, after using the mask product in above-mentioned application, skin moisture, skin elasticity, which have, to be continued
Increase, illustrating the product of the present invention has excellent water conservation, reduces the effect of wrinkle；Carry out analysis product from wrinkle depth, can
To further illustrate that the product of the lignanoid containing the fruit of Chinese magnoliavine has anti-aging effects.
Above example only shows several embodiments of the present invention, therefore its description is more specific and detailed, but can not be
And it is interpreted as the limitation scope to patent of the present invention.It should be noted that：It is any to be engaged in professional and technical personnel, all have the ability
Using the technology contents having revealed that, some are made to it and changes or modifies, turns into the equivalent embodiment of equivalent variations.According to this hair
Bright technical spirit, simple modification, equivalent change and modification is carried out to the present invention, belonged in the range of technical solution of the present invention.
Claims (10)
1. a kind of preparation method of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity, it is characterised in that include following step
Suddenly：
(1) by fruit of Chinese magnoliavine residue, after 45 DEG C dry and crush, fruit of Chinese magnoliavine residue powder is obtained；
(2) by fruit of Chinese magnoliavine residue powder, the distilled water of 10 times of parts by weight is added, 1~2% skimmed milk power, 0.1~1% sucrose, is mixed
It is even；Ultraviolet irradiation sterilization 5min；
(3) Bifidobacteria powder is added after sterilizing, is mixed, is placed under 32 DEG C of constant temperature ventilation conditions and cultivates 3d, will after fermentation ends
Solid constituent takes out drying, and must ferment fruit of Chinese magnoliavine residue powder；
(4) will ferment fruit of Chinese magnoliavine residue powder, add 70~80% ethyl acetate immersion 20min of 8 times of parts by weight, face by super
After boundary CO2 extractions, fruit of Chinese magnoliavine residue extract is obtained；
(5) by fruit of Chinese magnoliavine residue extract, purifying is isolated, is concentrated under reduced pressure, be dried in vacuo, obtain fruit of Chinese magnoliavine lignanoid.
2. preparation method according to claim 1, it is characterised in that the fruit of Chinese magnoliavine residue is fruit of Chinese magnoliavine seed, fruit
Meat, rattan, leaf, the root remaining residue after water extracts.
3. preparation method according to claim 1, it is characterised in that in step (1), the fruit of Chinese magnoliavine residue powder needed
50~70 mesh sieves.
4. preparation method according to claim 1, it is characterised in that in step (4), the SCF-CO 2 parameter
For：36~43 DEG C of constant temperature extractings, extracting pressure 23Mpa, 33 DEG C of extraction temperature, extraction time 1.5h, CO2 flow 5L/min.
5. preparation method according to claim 1, it is characterised in that in step (5), isolate and purify the side of fruit of Chinese magnoliavine residue
Method is the macroreticular resin absorbing method using following parameter：The applied sample amount of 4~5 times of bed volumes, elution flow rate 1mL/min, distillation
Water elution volume is 5~7 times of bed volumes, and eluant, eluent is 85~95% ethanol, and eluting agent is 5 times of bed volumes.
6. the fruit of Chinese magnoliavine with antioxidant and anti-aging activity according to obtained by any one of Claims 1 to 5 preparation method
Lignanoid.
7. the answering in cosmetics are prepared of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity according to claim 6
With.
8. the answering in cosmetics are prepared of the fruit of Chinese magnoliavine lignanoid with antioxidant and anti-aging activity according to claim 7
With, it is characterised in that the State of cosmetics includes cream, emulsion, aqua.
9. a kind of cosmetics, it is characterised in that include the fruit of Chinese magnoliavine with antioxidant and anti-aging activity described in claim 6
Lignanoid.
10. a kind of facial mask, it is characterised in that the facial mask includes following mass percent composition：Having described in claim 6
Antioxidant and anti-aging activity fruit of Chinese magnoliavine lignanoid 0.2~2%, glycerine 3~8%, butanediol 3~8%, propane diols 3~8%,
Ethoxy urea 1~6%, niacinamide 1~5%, Sodium Hyaluronate 0.02~0.06%, Phenoxyethanol 0.3~0.8%, oxybenzene first
Ester 0.1~0.3%, Cremophor RH40 0.01~0.5%, methylisothiazolinone 0.002~0.008%, iodine propine
Alcohol butyl mephenesin Carbamate 0.01~0.03%, deionized water surplus.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111875483A (en) * | 2020-08-25 | 2020-11-03 | 长春工业大学 | Method for extracting lignans compounds from schisandra chinensis |
| CN111875483B (en) * | 2020-08-25 | 2022-11-29 | 长春工业大学 | Method for extracting lignanoid compounds from schisandra chinensis |
| CN112315867A (en) * | 2020-11-30 | 2021-02-05 | 肽源（广州）生物科技有限公司 | Schisandra fruit rhodotorula mucilaginosa fermentation extract and preparation method and application thereof |
| CN112315867B (en) * | 2020-11-30 | 2022-11-18 | 肽源（广州）生物科技有限公司 | Schisandra fruit rhodotorula mucilaginosa fermentation extract and preparation method and application thereof |
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