CN107383230A - The extracting method of Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium and application - Google Patents
The extracting method of Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium and application Download PDFInfo
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- CN107383230A CN107383230A CN201710727134.7A CN201710727134A CN107383230A CN 107383230 A CN107383230 A CN 107383230A CN 201710727134 A CN201710727134 A CN 201710727134A CN 107383230 A CN107383230 A CN 107383230A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
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Abstract
The invention discloses a kind of extracting method of Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium and application, procedure below is included:(1) wilsonii residue is dried, after pulverizing and sieving, treats that it is volatilized after being soaked one day using ether, obtain wilsonii residue powder;(2) wilsonii residue powder is taken, adds deionized water, and adds protease, water bath with thermostatic control extraction, obtains wilsonii residue leaching liquor;(3) stood overnight after wilsonii residue leaching liquor is mixed with isometric organic digestion agent, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor is collected after separating impurity;(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using ethanol, take precipitation to be further condensed into medicinal extract, obtain Radix Et Caulis Acanthopanacis Senticosi polysaccharide.The present invention can strengthen the activity of antioxidase, play antioxidation, can reduce the content of lipid peroxidation product MDA, and then mitigate the damage that ultraviolet irradiation is brought.
Description
Technical field
The present invention relates to cosmetic field, and in particular to a kind of Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium carries
Take method and application.
Background technology
Wilsonii be Araliaceae wilsonii drying root and rhizome river, stem, leaf can also make it is medicinal, also known as slender acanthopanax ginseng.
Wilsonii has Multiple components, including eleutheroside, Radix Et Caulis Acanthopanacis Senticosi polysaccharide, wilsonii albumen, wilsonii flavones etc..With damage-retardation
Wound, anti-aging, radioresistance and antifatigue and other effects.
Due to glycoside, dissolubility and stability are poor in water, facile hydrolysis, therefore the method for traditional extraction eleutheroside
For alcohol extracting.And Radix Et Caulis Acanthopanacis Senticosi polysaccharide alcohol-soluble is poor, when extracting acanthopanax senticosus saponins using alcohol extracting method, a large amount of Radix Et Caulis Acanthopanacis Senticosi polysaccharides are still
Stay in residue.Therefore, we can further be extracted wilsonii alcohol extracting residue, so as to obtain Radix Et Caulis Acanthopanacis Senticosi polysaccharide.Thorn
Slender acanthopanax polysaccharide has good scavenging action to the reactive oxygen species in cell, while can also strengthen the activity of all kinds of antioxidases,
Reach preferable antioxidation;Meanwhile the generation by cytolipin peroxidating caused by suppressing ultraviolet and reduction fat
The content of matter Peroxidation Product MDA, and then reduce the damage that ultraviolet irradiation is brought.
The research to wilsonii is concentrated mainly on component and effect at present, extracting method and patent Shen to wilsonii residue
The record of correlation is not retrieved please.
The content of the invention
Based on this, it is an object of the invention to provide a kind of extraction of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium
Method and application.
To achieve the above object, the present invention adopts the following technical scheme that:
A kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, includes procedure below:
(1) wilsonii residue is dried to constant mass at 35~45 DEG C, after crossing 70~90 mesh sieves after crushing, uses second
Ether treats that it is volatilized after soaking one day, obtains wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 20 times of deionized waters, and adds protease, water bath with thermostatic control is carried out at 80 DEG C
Extraction 8 hours, obtains wilsonii residue leaching liquor;
(3) stand overnight after wilsonii residue leaching liquor is mixed with isometric organic digestion agent, collected after separating impurity
Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor;
(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using ethanol, take precipitation to be further condensed into leaching
Cream, obtain Radix Et Caulis Acanthopanacis Senticosi polysaccharide.
In wherein some embodiments, the wilsonii residue is that wilsonii is remaining residual after alcohol extracting other compositions
Slag.
In wherein some embodiments, protease in the step (2) is 0.3~5% protease.
In wherein some embodiments, organic digestion agent in the step (3) is volume ratio 4:1 chloroform, n-butanol
Mixed liquor.
In wherein some embodiments, the ethanol in the step (4) is the ethanol solution of 75~95% concentration.
Present invention also offers the wilsonii with radioresistance activity of fighting against senium according to obtained by above-mentioned preparation method is more
Sugar.
Present invention also offers the above-mentioned Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium.
In wherein some embodiments, the State of cosmetics includes cream, emulsion, aqua.
Present invention also offers a kind of cosmetics, including the foregoing wilsonii with radioresistance activity of fighting against senium is more
Sugar.
Present invention also offers a kind of anti-aging suncream, includes following mass percent composition:Have as previously described
The Radix Et Caulis Acanthopanacis Senticosi polysaccharide 0.5~5% of radioresistance activity of fighting against senium, glycerine 3~8%, hydrolyzed pearl 1~5%, glycine betaine 3~8%,
Acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer 0.1~0.5%, parahydroxyacet-ophenone 0.5~1%, panthenol
3~8%, allantoin 0.5~0.8%, triethanolamine 0.2~0.4%, propane diols 3~8%, pungent glycol 3~8%, ethylhexyl
Glycerine 1~1.5%, PEG-50 rilanit specials 0.03~0.1%, nonyl phenol polyethers -140.1~0.5%, more than deionized water
Amount.
It is the device have the advantages that as follows:The present invention, which is largely tested by inventor, and experience is preferred prepares
The various conditions of method, the preparation method optimized, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide obtained using preparation method of the present invention, content
Height, properties of product are stable, can add in a variety of skin-protection products, such as cream, emulsion, aqua.The present invention can strengthen antioxygen
Change the activity of enzyme, play antioxidation, additionally it is possible to reduce the content of lipid peroxidation product MDA, and then mitigate ultraviolet photograph
Penetrate the damage brought.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated:
All the components appeared in text are INCI (International Nomenclature Cosmetic
Ingredient), i.e., international cosmetic raw material is obviously said to its defined title.
Embodiment 1
A kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, includes procedure below:
(1) remaining residue after wilsonii alcohol extracting is dried to constant mass at 40 DEG C, after crossing 80 mesh sieves after crushing, made
Treat that it is volatilized after being soaked one day with ether, obtain wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 20 times of deionized waters, and adds 2.5% protease, constant temperature is carried out at 80 DEG C
Water-bath extracts 8 hours, obtains wilsonii residue leaching liquor;
(3) by wilsonii residue leaching liquor and isometric volume ratio 4:1 chloroform, n-butanol mixed liquor were placed after mixing
At night, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor is collected after separating impurity;
(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using 80% ethanol, take precipitation further to be concentrated
Into medicinal extract, Radix Et Caulis Acanthopanacis Senticosi polysaccharide is obtained.
Embodiment 2
A kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, includes procedure below:
(1) remaining residue after wilsonii alcohol extracting is dried to constant mass at 35 DEG C, after crossing 70 mesh sieves after crushing, made
Treat that it is volatilized after being soaked one day with ether, obtain wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 20 times of deionized waters, and adds 0.3% protease, constant temperature is carried out at 80 DEG C
Water-bath extracts 8 hours, obtains wilsonii residue leaching liquor;
(3) by wilsonii residue leaching liquor and isometric volume ratio 4:1 chloroform, n-butanol mixed liquor were placed after mixing
At night, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor is collected after separating impurity;
(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using 75% ethanol, take precipitation further to be concentrated
Into medicinal extract, Radix Et Caulis Acanthopanacis Senticosi polysaccharide is obtained.
Embodiment 3
A kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, includes procedure below:
(1) remaining residue after wilsonii alcohol extracting is dried to constant mass at 45 DEG C, after crossing 90 mesh sieves after crushing, made
Treat that it is volatilized after being soaked one day with ether, obtain wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 20 times of deionized waters, and adds 5% protease, thermostatted water is carried out at 80 DEG C
Bath extraction 8 hours, obtains wilsonii residue leaching liquor;
(3) by wilsonii residue leaching liquor and isometric volume ratio 4:1 chloroform, n-butanol mixed liquor were placed after mixing
At night, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor is collected after separating impurity;
(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using 95% ethanol, take precipitation further to be concentrated
Into medicinal extract, Radix Et Caulis Acanthopanacis Senticosi polysaccharide is obtained.
Comparative example 1
A kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, includes procedure below:
(1) remaining residue after wilsonii alcohol extracting is dried to constant mass at 50 DEG C, after crossing 60 mesh sieves after crushing, made
Treat that it is volatilized after being soaked one day with ether, obtain wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 10 times of deionized waters, and adds 3% protease, thermostatted water is carried out at 85 DEG C
Bath extraction 5 hours, obtains wilsonii residue leaching liquor;
(3) by wilsonii residue leaching liquor and isometric volume ratio 3:1 chloroform, n-butanol mixed liquor were placed after mixing
At night, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor is collected after separating impurity;
(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using 50% ethanol, take precipitation further to be concentrated
Into medicinal extract, Radix Et Caulis Acanthopanacis Senticosi polysaccharide is obtained.
Comparative example 2
A kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, includes procedure below:
(1) remaining residue after wilsonii alcohol extracting is dried to constant mass at 40 DEG C, after crossing 80 mesh sieves after crushing, made
Treat that it is volatilized after being soaked one day with ether, obtain wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 20 times of deionized waters, water bath with thermostatic control is carried out at 80 DEG C and is extracted 8 hours, is obtained
Wilsonii residue leaching liquor;
(3) by wilsonii residue leaching liquor and isometric volume ratio 4:1 chloroform, n-butanol mixed liquor were placed after mixing
At night, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor is collected after separating impurity;
(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using 80% ethanol, take precipitation further to be concentrated
Into medicinal extract, Radix Et Caulis Acanthopanacis Senticosi polysaccharide is obtained.
Comparative example 3
A kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, includes procedure below:
(1) remaining residue after wilsonii alcohol extracting is dried to constant mass at 40 DEG C, after crossing 80 mesh sieves after crushing, made
Treat that it is volatilized after being soaked one day with ether, obtain wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 20 times of deionized waters, water bath with thermostatic control is carried out at 80 DEG C and is extracted 8 hours, is obtained
Wilsonii residue leaching liquor;
(3) wilsonii residue leaching liquor is stood overnight, the Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid in lower floor is collected after separating impurity;
(4) precipitated using 80% ethanol, take precipitation to be further condensed into medicinal extract, obtain Radix Et Caulis Acanthopanacis Senticosi polysaccharide.
Interpretation of result:Embodiment 1-3 and comparative example 1-3 preparation condition have the wilsonii of radioresistance activity of fighting against senium more
The content such as table 1 below of sugar.
Table 1
From the data analysis of table 1:The content of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide obtained under embodiment 1-3 preparation condition substantially compares
The content of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide obtained under ratio 1-3 preparation condition is substantially higher;Comparative example 1 changes all of preparation technology
Parameter, the content that Comparative result example 1 obtains Radix Et Caulis Acanthopanacis Senticosi polysaccharide are significantly lower than embodiment 1;It is no in comparative example 2 to add protease,
But the extracted amount of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide of comparative example 2 is substantially fewer than embodiment 1, illustrate that protease can make polysaccharide component be easier quilt
Extraction, and then improve extracted amount;The preparation condition of comparative example 3 is to use ethanol precipitation after water extraction, but Radix Et Caulis Acanthopanacis Senticosi polysaccharide content ratio
Embodiment 1 is low, illustrates that the purpose for improving raw material availability can be reached using the method for enzymolysis water extract-alcohol precipitation;And pass through repeatedly
Change the experiment such as technological parameter, step, obtain the preparation method that the present invention protects.
The Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium that embodiment 1-3 is prepared, which is applied, is preparing anti-aging
In suncream, table 2 specific as follows.
Table 2
Sun-proof function is verified
Principle:During TYR changes into melanin, the catalytic action of TYR enzyme occurs mainly in TYR enzyme
It is converted into DOPA and DOPA changes into the process of DOPA quinone, DOPA quinone is a kind of coloring matter, there is absorption at 475nm, can profit
With spectrophotometer measurement content.L-DOPA is substrate, determines the TYR enzyme inhibitory activity of suncream.
Method:It is accurate to draw suncream and the liquid of various concentrations is diluted to phosphate buffer, substance is diluted to respectively
1 times, 3 times, 5 times, 7 times of product, adds 300 μ/mL TYR enzyme solution, continues to cultivate after being incubated 10 minutes in 30 DEG C of water-baths
Ten minutes, determine its light absorption value in 475nm immediately.
As a result such as table 3 below.
Table 3
Group | 1 times | 3 times | 5 times | 7 times | Blank |
Inhibiting rate % | 85.8 | 80.1 | 78.1 | 72.5 | 9.8 |
From the data analysis of table 3:Suncream plays the role of good suppression tyrosinase, and it is sun-proof to reflect its indirectly
Effect is good.
Anti-radiation effect is verified
Healthy experiment mice 30, body weight 20-25g is chosen, female, is randomly divided into following 3 groups:Radix Et Caulis Acanthopanacis Senticosi polysaccharide group, prevent
Shine white group, blank control group.Mouse dorsal body setae is shaved only, area 2cm × 2cm.In addition to blank control group, by 3 groups of mouse in ultraviolet
(UVA and UVB ultraviolet lamp tubes are produced by Hefei Sai Fan testing equipments Co., Ltd, model UVA-340 and UVB-313 type) mould under lamp
Plan solar radiation, irradiation distance 50cm, 1h/d, 5 times a week, continuous 2 weeks.Before ultraviolet irradiation 20min, mouse is equal in hair removal section
The even trial target smeared corresponding group and prepared.After off-test, every animal UV-irradiating portion skin holostrome 1g is taken, is prepared
Skin histology homogenate, it is horizontal that MDA, SOD, GSH-Px, CAT, Hyp are detected respectively.UV-irradiating portion skin histology SOD,
GSH-Px, CAT and MDA content statistical result are shown in Table 4.
Table 4
Compared with control group 1, * P < 0.05, * * P < 0.01.
From the data analysis of table 4:Radix Et Caulis Acanthopanacis Senticosi polysaccharide, skin histology SOD, GSH-Px, CAT and Hyp of suncream group
Level is remarkably decreased (P < 0.05 or P < 0.01), the horizontal significantly rises (P < 0.01) of MDA, it is seen that Radix Et Caulis Acanthopanacis Senticosi polysaccharide has anti-
It is radiation activated, applied in suncream so that suncream has radioresistance effect.
Senile-resistant efficacy is verified
After above-mentioned radioresistance off-test, UV-irradiating portion skin 0.3cm × 0.3cm is taken, not through ultraviolet irradiation
Same batch mouse ketone position skin 0.3cm × 0.3cm, fixed respectively with 10% formalin, FFPE, section,
HE is dyed, using observation by light microscope skin histopathology form.
As a result:It has been shown that, blank group ultraviolet irradiation mouse skin are observed under skin texture light microscope (10 × 40)
Substantially thicken, institutional framework is imperfect, fibr tissue arrangement disorder, phenomenon of rupture occurs, cell shrinkage, shows as aging mark
As.Radix Et Caulis Acanthopanacis Senticosi polysaccharide group and suncream group mouse skin cuticula are thin, and epidermal tissue's structural integrity, each confluent monolayer cells are clear, corium
Layer fibr tissue is uniformly distributed in wavy, and cells show power is strong, or even than without the mouse skin vigor by ultraviolet irradiation
By force.The anti-aging effects of Radix Et Caulis Acanthopanacis Senticosi polysaccharide and suncream are reflected indirectly.
Above example only shows several embodiments of the present invention, therefore its description is more specific and detailed, but can not be
And it is interpreted as the limitation scope to patent of the present invention.It should be noted that:It is any to be engaged in professional and technical personnel, all have the ability
Using the technology contents having revealed that, some are made to it and changes or modifies, turns into the equivalent embodiment of equivalent variations.According to this hair
Bright technical spirit, simple modification, equivalent change and modification is carried out to the present invention, belonged in the range of technical solution of the present invention.
Claims (10)
1. a kind of preparation method of the Radix Et Caulis Acanthopanacis Senticosi polysaccharide with radioresistance activity of fighting against senium, it is characterised in that include procedure below:
(1) wilsonii residue is dried to constant mass at 35~45 DEG C, after crossing 70~90 mesh sieves after crushing, soaked using ether
Bubble treats that it is volatilized after one day, obtains wilsonii residue powder;
(2) wilsonii residue powder is taken, adds 20 times of deionized waters, and adds protease, water bath with thermostatic control extraction 8 is carried out at 80 DEG C
Hour, obtain wilsonii residue leaching liquor;
(3) stood overnight after wilsonii residue leaching liquor is mixed with isometric organic digestion agent, collect and be in after separation impurity
The Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid of lower floor;
(4) take Radix Et Caulis Acanthopanacis Senticosi polysaccharide liquid to be precipitated after being concentrated under reduced pressure using ethanol, take precipitation to be further condensed into medicinal extract, obtain
To Radix Et Caulis Acanthopanacis Senticosi polysaccharide.
2. preparation method according to claim 1, it is characterised in that the wilsonii residue is that wilsonii passes through alcohol extracting
Remaining residue after other compositions.
3. preparation method according to claim 1, it is characterised in that in step (2), the protease is 0.3~5%
Protease.
4. preparation method according to claim 1, it is characterised in that in step (3), organic digestion agent is volume ratio
4:1 chloroform, n-butanol mixed liquor.
5. preparation method according to claim 1, it is characterised in that in step (4), the ethanol is 75~95% concentration
Ethanol solution.
6. the wilsonii with radioresistance activity of fighting against senium according to obtained by any one of Claims 1 to 5 preparation method
Polysaccharide.
7. the Radix Et Caulis Acanthopanacis Senticosi polysaccharide according to claim 6 with radioresistance activity of fighting against senium.
8. the Radix Et Caulis Acanthopanacis Senticosi polysaccharide according to claim 7 with radioresistance activity of fighting against senium answering in cosmetics are prepared
With, it is characterised in that the State of cosmetics includes cream, emulsion, aqua.
9. a kind of cosmetics, it is characterised in that include the wilsonii with radioresistance activity of fighting against senium described in claim 6
Polysaccharide.
10. a kind of anti-aging suncream, it is characterised in that the anti-aging suncream includes following mass percent composition:Power
Profit require the Radix Et Caulis Acanthopanacis Senticosi polysaccharide 0.5~5% with radioresistance activity of fighting against senium described in 6, glycerine 3~8%, hydrolyzed pearl 1~
5%th, glycine betaine 3~8%, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer 0.1~0.5%, para hydroxybenzene
Ethyl ketone 0.5~1%, panthenol 3~8%, allantoin 0.5~0.8%, triethanolamine 0.2~0.4%, propane diols 3~8%, Xin Gan
Alcohol 3~8%, Sensiva SC50 1~1.5%, PEG-50 rilanit specials 0.03~0.1%, nonyl phenol polyethers -14 0.1~
0.5%th, deionized water surplus.
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CN109021139A (en) * | 2018-08-10 | 2018-12-18 | 廊坊市思丁生物科技发展有限公司 | The extraction and purification method of Radix Et Caulis Acanthopanacis Senticosi polysaccharide |
CN111961141A (en) * | 2020-07-20 | 2020-11-20 | 广州青岚生物科技有限公司 | Acanthopanax senticosus homogeneous polysaccharide and preparation method and application thereof |
CN114276408A (en) * | 2022-01-18 | 2022-04-05 | 长春中医药大学 | Extraction method and application of acanthopanax senticosus glycoprotein |
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