CN106937918A - A kind of natural radiation-preventing composition and its application - Google Patents

A kind of natural radiation-preventing composition and its application Download PDF

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Publication number
CN106937918A
CN106937918A CN201710212195.XA CN201710212195A CN106937918A CN 106937918 A CN106937918 A CN 106937918A CN 201710212195 A CN201710212195 A CN 201710212195A CN 106937918 A CN106937918 A CN 106937918A
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radiation
parts
composition
phases
skin
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鲁翠容
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Foshan Huili Cosmetics Science And Technology Co Ltd
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Foshan Huili Cosmetics Science And Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/31—Hydrocarbons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/342—Alcohols having more than seven atoms in an unbroken chain
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/345—Alcohols containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/37—Esters of carboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists

Abstract

The invention discloses a kind of natural radiation-preventing composition and its application, combine the plain amino acid of species mycetocyte, phycobniliprotein, laver amylose and Tea Polyphenols compounding use, by absorbing the approach such as ultraviolet radiation, suppression lipid oxidation, removing free radical, absorption decomposition radiant light, suppression tyrosinase activity, absorption electron radiation, nutrition reparation skin, maintenance one, each component acts synergistically, natural safety so that composition produces significant radiation protection.Composition is applied in cosmetic emulsion, the radiation proof emulsion made, not only can effectively absorb UV A and UV B ultraviolet radioactives, can be with absorption of electromagnetic radiation.There is protection body immune system function, hematopoieticmicroenviron-ment, nutrition skin can be improved, skin more consolidation is allowed, it is more flexible, so as to effectively prevent and reduce infringement of the radiation to skin, improve the self-protection ability of skin.

Description

A kind of natural radiation-preventing composition and its application
Technical field
The present invention relates to cosmetic field, and in particular to a kind of natural radiation-preventing composition and its application.
Background technology
Used with the development of science and technology, the electronic product such as computer and mobile phone has become indispensable life in people's life Product, also give people to bring various infringements, particularly electromagnetic radiation to the skin of people while these electronic products give people to bring convenience Skin causes very big injury.There are some researches show electromagnetic radiation can induce generation free radical, increase the interior free-radical contents of human body It is many.Two free radicals can have certain infringement to skin, be mainly shown as：(1) serum antiprotease in skin is made to lose activity； (2) Skin Cell film is destroyed (3) injured skin gene causes appearance and the savings of cytometaplasia.What if cell membrane was destroyed Speed is more than cytothesis speed, and function of organization will be affected and change, and cause lesion etc.；Occur in subcutaneous collagen group Knit, skin can be made to follow the string.If human body is long-term by such injury, caused result is exactly various chronic diseases and people The aging of body, so as to cause color spot, inflammation, accelerate skin aging, destruction immune system and DNA destructions etc..In addition, solar radiation Also skin aging can be accelerated, make skin the phenomenons such as dark yellow, color spot, wrinkle, pore be thick occur.Radiation is ubiquitous, can be rated as " hidden Shape killer ", so a safe and efficient radiation protection product of research and development is necessary.
At present, it is many using radiation proof concept as attraction on the market, but it is practically without anti-radiation effect or radiation proof effect Fruit substantially, does not mislead deception consumer；Or the composition of the rapid whitening of addition energy, such as heavy metal lead, such cosmetics safety It is low, certain harm can be caused to human body.
The content of the invention
Regarding to the issue above, the technical problem to be solved in the present invention is：A kind of natural safe radiation-preventing composition is provided, And composition can effectively prevent and reduce infringement of the radiation to skin, so as to make skin more healthy.
To reach the effect above, the scheme that the present invention is provided is as follows：
A kind of natural radiation-preventing composition is consisted of the following composition：0.01-3 parts of amino acid of class mycetocyte element, phycobniliprotein 0.1-4.5 parts, 0.01-2.5 parts of laver amylose, 0.1-2.4 parts of Tea Polyphenols.
Preferably, the composition is consisted of the following composition：0.1-1.5 parts of amino acid of class mycetocyte element, phycobniliprotein 1.5- 3.5 parts, 0.1-1.5 parts of laver amylose, 1.5-2 parts of Tea Polyphenols.
Most preferably, the composition is consisted of the following composition：Class mycetocyte element .3 parts of amino acid/11,2.5 parts of phycobniliprotein, 0.5 part of laver amylose, 1.8 parts of Tea Polyphenols.
The plain amino acid of the class mycetocyte, phycobniliprotein, laver amylose are extracted from seaweed and are made.
Present invention also offers application of the natural radiation-preventing composition in cosmetics are prepared, and the cosmetics are breast Liquid.
Described emulsion is consisted of the following composition：It is radiation-preventing composition 0.22-12.40%, glycerine 6.00-8.00%, hard Resin acid 1.50-2.50%, stearine 1.00-2.00%, stearyl alcohol 1.00-2.00%, isopropyl myristate 2.00- 3.00%th, octanoic acid/certain herbaceous plants with big flowers acid glycerol three ester 1.00-2.00%, Dormant oils 1.00-2.00%, OXYBENZONE 1.00-2.00%, C12- 13 sodium alkyl sulfate 0.50-1.00%, the 0.50-1.00% of laureth -20, allantoin 0.30-0.50%, essence 0.10- 0.30%th, butyl p-hydroxybenzoate 0.10-0.30%, ethyl-para-hydroxybenzoate 0.10-0.30%, hydroxyethyl cellulose 0.05-0.10%, deionized water surplus.
The preparation method of described emulsion, comprises the following steps：
1) by stearic acid, stearine, stearyl alcohol, octanoic acid/certain herbaceous plants with big flowers acid glycerol three ester, Dormant oils, OXYBENZONE, laruyl alcohol Polyethers -20, butyl p-hydroxybenzoate mixing put into oil phase pot heating stirring to 85 degree as A phases, and stirring and dissolving is protected afterwards completely Temperature is stand-by；
2) glycerine, C12-13 sodium alkyl sulfates, allantoin, ethyl-para-hydroxybenzoate, deionized water are mixed and is used as B phases Heating stirring is to 85 degree in addition aqueous phase pot, heat preservation for standby use after stirring and dissolving is complete；
3) emulsification pot is preheated to 60-65 degree, B phases are first pumped into by 50 revs/min of mixing speed, then A phases are pumped into, and are stirred Mix 30 minutes, temperature is maintained at 85 degree；
4) C is added to emulsification pot, homogeneous 3 minutes, insulated and stirred 30 minutes, temperature is maintained at 80-85 degree, mixing speed 35 revs/min；The C phases are hydroxyethyl cellulose；
5) 42 degree of addition D phases are cooled to, insulated and stirred 10 minutes, 25-30 revs/min of mixing speed is eventually adding E phases, Insulation 10-20 minutes；The D phases are composition described in claim 1, and the E phases are butyl p-hydroxybenzoate, para hydroxybenzene Ethyl formate；
6) stirring is cooled to 40 degree of dischargings.
Effect or feature to the constituent part of the present invention do following introduction：
Class mycetocyte element amino acid is extracted from seaweed, using cyclonene as basic framework, with different type amino The major class water-soluble substances that acid is formed by amine condensation.Because active group is deposited in conjugated double bond and different side chains There is strong absorbability in 310~360nm ultraviolet region in, class mycetocyte element amino acid, can not only effectively absorb ultraviolet Light radiation, moreover it is possible to which the energy of absorption is dissipated rather than passed to the biomolecule of sensitivity, and will not be formed and cause oxygen to coerce Urgent active oxygen species.Secondly, class mycetocyte element amino acid have good scavenging hydroxyl and superoxide anion ability and Stronger suppression lipid oxidation ability.Therefore, the plain amino acid of class mycetocyte can effectively reduce damage of the UVA radiation to skin, be A kind of safe and harmless pure natural antiradiation agent.
Phycobniliprotein is a kind of water solubility, while having the chromoprotein of natural activity, is widely present in marine alga body.Algae Biliprotein is having stronger absorption decomposition to the larger radiant light section of human body skin injury, by absorption of electromagnetic radiation, Energy is converted, the repair system of active cell eliminates or reduced the anion that radiation is produced on DNA, reaches protection electromagnetism spoke The mesh penetrated, so as to reduce injury of the electromagnetic radiation to human body skin.
Laver amylose belongs to galactan sulfuric ester, is mainly made up of galactolipin, 3,6- inner ethers galactolipin and sulfate etc., The 20%~40% of seaweed dry mass is accounted for, is one of main component of seaweed.Laver amylose can strengthen immunologic function, protection and make Blood system and anti-oxidant, with good radiation resistance.Compared with other antiradiation drugs, polysaccharide is natural active matter Matter, it is nontoxic, and its metabolite can provide required nutriment for body.Research is found recently, and laver amylose also has A variety of special biological functions such as anti-aging, anticoagulation, antithrombotic, anti-oxidant.
Tea Polyphenols can absorb 200~400nm ultraviolet, be the natural of a class high-efficiency low-toxicity, with anti- The bioactivity such as oxidation, elimination free radical.The oxidation-reduction potential of Tea Polyphenols is very low, possesses multiple phenolic hydroxyl groups, using the teaching of the invention it is possible to provide matter Son is combined to eliminate the free radical of internal excess with the free radical that radiation is produced, it is to avoid the damage of large biological molecule, so as to play Protective effect.Meanwhile, Tea Polyphenols can strengthen glutathione peroxidase, catalase, fat reductase and glutathione-s- and turn Move the activity of enzyme, Scavenging activity of enhancing body itself Antioxidative Defense System to excessive free radicals.Secondly, Tea Polyphenols can be with Effectively suppress tyrosinase activity, reduce the formation of melanin, there is Whitening, spot.
The device have the advantages that as follows：(1) radiation-preventing composition compounding use of the invention, ultraviolet by absorbing Light radiation, suppress lipid oxidation, remove free radical, absorb decompose radiant light, suppress tyrosinase activity, absorb electron radiation, The approach such as skin, maintenance one, each component synergy, natural safety are repaired in nutrition so that composition produces significant anti-spoke The effect of penetrating；(2) the plain amino acid of class mycetocyte stresses the radiation of pre- preventing ultraviolet, and phycobniliprotein stresses pre- anti-electromagnetic radiation, and two effects are closed One, the characteristics of imparting the radiation proof of product wide spectrum；(3) radiation proof emulsion of the invention, not only can effectively absorb UV-A and UV-B ultraviolet radioactives, can be with absorption of electromagnetic radiation.Wherein plant extracts composition has opsonic action, can protect immunity of organism Systemic-function, can improve hematopoieticmicroenviron-ment, nutrition skin, allow skin more consolidation, more flexible, so as to effectively prevent and reduce The infringement to skin is radiated, the self-protection ability of skin is improved；(4), can be more using glycerine and fatty alcohol as moisturizing ingredient Deep water supplement, promotes the absorption of active ingredient well；Taken using isopropyl myristate, Dormant oils and octanoic acid/certain herbaceous plants with big flowers acid glycerol three ester Match somebody with somebody, there is good intersolubility, and the viscosity of system can be made, so that emulsion is good in the ductility of skin, promote infiltration.
Brief description of the drawings
Fig. 1：Scavenging activity of the radiation-preventing composition to DPPH.
Fig. 2：Inhibitory action of the radiation-preventing composition to TYR enzyme activity.
Embodiment
The present invention is further elaborated by the following specific embodiment.
The following component title occurred in text is INCI (Internation Nomenclaure Cosmetic Ingredent, i.e. international cosmetic raw material name) institute to defined title：Octanoic acid/certain herbaceous plants with big flowers acid glycerol acid esters.
The made emulsion formulations of radiation-preventing composition of the present invention of embodiment 1~5 are shown in Table one
Table one
The method of the emulsion of embodiment 1~5, it is characterised in that the described method comprises the following steps：
1) by stearic acid, stearine, stearyl alcohol, octanoic acid/certain herbaceous plants with big flowers acid glycerol three ester, Dormant oils, OXYBENZONE, laruyl alcohol Polyethers -20, butyl p-hydroxybenzoate mixing put into oil phase pot heating stirring to 85 degree as A phases, and stirring and dissolving is protected afterwards completely Temperature is stand-by；
2) glycerine, C12-13 sodium alkyl sulfates, allantoin, ethyl-para-hydroxybenzoate, deionized water are mixed and is used as B phases Heating stirring is to 85 degree in addition aqueous phase pot, heat preservation for standby use after stirring and dissolving is complete；
3) emulsification pot is preheated to 60 degree, B phases are first pumped into by 50 revs/min of mixing speed, then A phases are pumped into, stirring 30 Minute, temperature is maintained at 85 degree；
4) C is added to emulsification pot, homogeneous 3 minutes, insulated and stirred 30 minutes, temperature is maintained at 80-85 degree, mixing speed 35 revs/min；The C phases are hydroxyethyl cellulose；
5) 42 degree of addition D phases are cooled to, insulated and stirred 10 minutes, 30 revs/min of mixing speed is eventually adding E phases, is incubated 20 minutes；The D phases are composition described in claim 1, and the E phases are butyl p-hydroxybenzoate, P-hydroxybenzoic acid second Ester and essence；
6) stirring is cooled to 40 degree of dischargings.
Comparative example 1
Difference with embodiment 1 is not add laver amylose, and other preparation conditions and method are same as Example 1.
Comparative example 2
Difference with embodiment 1 is not add the plain amino acid of class mycetocyte, other preparation conditions and method and the phase of embodiment 1 Together.
Comparative example 3
Difference with embodiment 1 is not add phycobniliprotein, and other preparation conditions and method are same as Example 1.
Comparative example 4
Difference with embodiment 1 is not add Tea Polyphenols, and other preparation conditions and method are same as Example 1.
Comparative example 5
Difference with embodiment 1 is not add radiation-preventing composition, and other preparation conditions and method are same as Example 1.
Comparative example 6
Difference with embodiment 1 is that radiation-preventing composition percentage composition is 12.5%, other preparation conditions and method and reality Apply example 1 identical.
Radiation-preventing composition of the present invention removes DPPH capacity experimentals
Method and apparatus
Radiation-preventing composition (self-control) in embodiment one；95% ethanol (Chemical Reagent Co., Ltd., Sinopharm Group)；DPPH (Tokyo is melted into Co., Ltd., analyzes pure)；AB204-E electronic analytical balances (METTLE TOLEDO)；UV-1600 is ultraviolet Spectrophotometer (METTLE TOLEDO).
Black purple is presented in DPPH in absolute ethyl alcohol and ethanol-water system, there is maximum suction under 517nm and 525nm respectively Receive.Free radical scavenger can make purple gradually retreat, and determine DPPH content according to the change of absorbance and react freely Scavenging activity of the base scavenger to DPPH.
Precision weighs DPPH3.00mg, is dissolved with 95% ethanol and is settled to 100ml, and DPPH solubility is 20mg/L, is taken 4mlDPPH standard liquids, are positioned in test tube, add 1ml radiation-preventing composition solution, place after 30min, are determined in 517nm Absorbance A.DPPH clearance rates are determined according to following equation；
DPPH clearance rate=[1- (Ai-Aj)/Ac] × 100%
Ai：The absorbance of extract solution+4mlDPPH solution
Aj：The absorbance of extract solution+4ml95% ethanol
Ac：The absorbance of 1ml95% ethanol+4mlDPPH solution
DPPH Scavenging activities are represented with clearance rate, clearance rate is higher to represent that Scavenging activity is stronger, by radiation-preventing composition 5%, 10%, 15%, 20%, 25% is diluted to respectively with 95% ethanol, detects the ability that each concentration removes DPPH.As a result see Fig. 1, Fig. 1 abscissa are radiation-preventing composition concentration, and ordinate is DPPH clearance rates.
As can be seen from Figure 1 radiation-preventing composition has Scavenging activity to DPPH, and with concentration increase removing degree by Cumulative height, removing degree tends towards stability after concentration reaches 15%, and clearance rate reaches 87%.
Radiation-preventing composition of the present invention suppresses the experiment of TYR enzyme enzyme
Method and apparatus
Radiation-preventing composition of the present invention；Ph=6.8 phosphate buffer solutions (PBS solution), NaOH (Chinese medicines group chemistry Reagent Co., Ltd)；TYR enzyme, Tyrosinase EC1.14.18.19 (signa)；L-DOPA(AcrosOrganics)； AB204-E electronic analytical balances (METTLE TOLEDO)；UV-1600 ultraviolet specrophotometers (METTLE TOLEDO).
During TYR changes into melanin, the catalytic action of TYR enzyme occurs mainly in TYR enzyme and is converted into DOPA and DOPA change into the process of DOPA quinone, and DOPA quinone is a kind of coloring matter, has absorption at 475nm, using light splitting Photometer measurement content.L-DOPA is substrate, determines the TYR enzyme inhibitory activity of radiation-preventing composition of the present invention.
The testing sample composition of table two
Radiation-preventing composition extract solution of the present invention, 0.15% DOPA solution are drawn according to accurate shown in table two, is fully mixed Close uniform, 300 μ/mL TYR enzyme solution is added after being incubated 10 minutes in 30 DEG C of water-baths, continue cultivation ten minutes, immediately Its light absorption value is determined in 475nm.
The computing formula of TYR enzyme inhibiting rate (I) is as follows：
I=[(A-B)-(C-D)]/[A-B] × 100%
A:Not plus the absorbance A (A) surveyed of the enzyme-added mixed liquor of radiation-preventing composition；
B:Not plus radiation-preventing composition and not plus the absorbance A (B) surveyed of enzyme-added mixed liquor；
C：Plus radiation-preventing composition and enzyme-added absorbance A (C) mixed liquor, being surveyed；
D:Plus the absorbance A (D) that the not enzyme-added mixed liquor of radiation-preventing composition is surveyed.
Radiation-preventing composition sample is diluted to 5%, 10%, 15%, 20% with PBS respectively, 25% detects each concentration Suppress TYR enzyme activity.As a result Fig. 2 is seen, Fig. 2 abscissas are the concentration of radiation-preventing composition, and ordinate is to TYR enzyme Inhibiting rate.
There is the visible radiation-preventing composition concentration of Fig. 2 when 5% to 20%, radiation-preventing composition suppresses TYR enzyme activity Property increase with the growth of metering, when suppress TYR enzyme activity solubility reach 20% when, TYR enzyme inhibiting rate can reach 86%.
Anti-radiation effect is verified
Kunming kind healthy mice 156 is chosen, body weight 20-25g, female is randomly divided into following 10 groups：Control group 1~2, Embodiment 1~5, comparative example 1~6, every group 12.Mouse dorsal body setae is shaved only, area 2cm × 2cm.In addition to control group 2, it is with 13 Group mouse under uviol lamp (UVA and UVB ultraviolet lamp tubes by Hefei Sai Fan testing equipments Co., Ltd produce, model UVA-340 and UVB-313 types) simulation solar radiation, irradiation distance 50cm, 1h/d, 5 times a week, continuous 8 weeks.It is real before ultraviolet irradiation 20min Apply 1~5 group of mouse of example 1~5 and comparative example and uniformly smear radiation proof face cream prepared by correspondence group in hair removal section, control group 2 is straight Connect irradiation.After off-test, every animal UV-irradiating portion skin holostrome 1g is taken, skin histology homogenate is prepared, respectively MDA, SOD, GSH-Px, CAT, Hyp level of detection.In addition, taking UV-irradiating portion skin 0.3cm × 0.3cm, 10% formaldehyde Solution is fixed, FFPE, section, HE dyeing, using observation by light microscope skin histopathology form.
UV-irradiating portion skin histology SOD, GSH-Px, CAT and MDA content statistics
It is shown in Table 1.
Each group mice skin tissue SOD, GSH-Px, CAT and MDA comparision contents of table 1
Group n SOD(μmol/g) GSH-Px(μmol/g) CAT(μmol/g) MDA(μmol/g) Hyp(mg/g)
Embodiment 1 12 119.2±12.7* 121.8±10.4** 304.7±31.3* 20.1±2.5** 3.24±0.48**
Embodiment 2 12 118.5±11.4* 119.8±9.5** 302.9±30.2* 20.2±2.1** 3.25±0.35**
Embodiment 3 12 118.6±12.5* 121.3±10.3** 301.9±30.1* 20.1±1.9** 3.25±0.37**
Embodiment 4 12 118.1±11.1* 120.9±10.2** 301.5±30.5* 21.2±2.1** 3.24±0.35**
Embodiment 5 12 117.9±10.5* 121.5±9.8** 303.5±30.8* 20.5±2.3** 3.26±0.36**
Comparative example 1 12 108.2±11.2 100.6±10.1 275.4±26.3 28.6±2.8 2.65±0.28
Comparative example 2 12 109.3±10.3 102.4±11.2 285.7±28.5 27.4±2.5 2.58±0.27
Comparative example 3 12 107.6±11.2 102.7±10.3 276.3±27.7 28.6±2.8 2.61±0.26
Comparative example 4 12 108.2±11.7 102.2±10.9 278.3±27.9 28.9±2.9 2.64±0.28
Comparative example 5 12 103.5±10.3 99.5±8.5 260.2±26.5 29.5±2.6 2.51±0.29
Comparative example 6 12 112.7±10.3 114.7±10.3 278.5±26.9 26.5±2.4 2.54±0.27
Control group 1 12 100.5±11.2 97.1±10.6 252.7±25.3 32.2±3.5 2.45±0.35
Control group 2 12 139.2±14.8** 129.1±13.3** 319.8±30.7** 22.3±2.5** 3.51±0.5**
Compared with control group 1, * P ＜ 0.05, * * P ＜ 0.01.
By the result of the test of table 1 can be seen that control group 1 and control group 2 this two groups of skin histology SOD, GSH-Px, CAT, MDA and Hyp level are respectively provided with pole significant difference (P ＜ 0.01)；Compared with control group 1, the skin histology of embodiment 1~5 SOD, GSH-Px, CAT and Hyp level are remarkably decreased (P ＜ 0.05 or P ＜ 0.01), and MDA levels are significantly raised (P ＜ 0.01).It is right It is almost unchanged that ratio 5 is compared visible dermis tissue SOD, GSH-Px, CAT, MDA and Hyp with control group 1, it is seen that in the present invention Radiation-preventing composition is the principle active component of the radiation proof face cream of embodiment 1~5；The difference point of comparative example 1~4 and embodiment 1 Wei not add laver amylose, the plain amino acid of class mycetocyte not added, phycobniliprotein and Tea Polyphenols is not added, by comparative example 1~4 with The contrast visible dermis of control group 1 tissue SOD, GSH-Px, CAT and Hyp level have declined, and MDA levels have lifted height, still Not notable (P>0.1), then with embodiment 1~5 contrast visible, because radiation-preventing composition compatibility changes, radiation-proof effect is deteriorated, The plain amino acid of laver amylose, class mycetocyte, phycobniliprotein and Tea Polyphenols Synergistic in radiation-preventing composition of the present invention.
Display is observed under skin texture light microscope (10 × 40), the epidermis of comparative example 5 is substantially thickened, institutional framework Imperfect, there is phenomenon of rupture, it is seen that inflammatory cell infiltration in fibr tissue arrangement disorder, the irregular hyperplasia of sebaceous glands.Embodiment 1~5 cuticula is thin, epidermal tissue's structural integrity, and each confluent monolayer cells are clear, and skin corium fibr tissue is uniformly distributed in wavy.
Upper embodiment only illustrates embodiment of the present invention, does not have any formal limitation to the present invention；It is any Professional and technical personnel is engaged in, all has the ability to make some changes using the technology contents disclosed or modifies, become as equivalent The Equivalent embodiments of change, according to the technology of the present invention essence, simply modification, equivalent variations and modification are carried out to the present invention, is belonged to In the range of technical solution of the present invention.

Claims (8)

1. a kind of natural radiation-preventing composition, it is characterised in that the composition is consisted of the following composition：Class mycetocyte element amino acid 0.01-3 parts, 0.1-4.5 parts of phycobniliprotein, 0.01-2.5 parts of laver amylose, 0.1-2.4 parts of Tea Polyphenols.
2. natural radiation-preventing composition according to claim 1, it is characterised in that the composition is by following component group Into：0.1-1.5 parts of amino acid of class mycetocyte element, 1.5-3.5 parts of phycobniliprotein, 0.1-1.5 parts of laver amylose, 1.5-2 parts of Tea Polyphenols.
3. natural radiation-preventing composition according to claim 1, it is characterised in that the composition is by following component group Into：.3 parts of amino acid/11 of class mycetocyte element, 2.5 parts of phycobniliprotein, 0.5 part of laver amylose, 1.8 parts of Tea Polyphenols.
4. the natural radiation-preventing composition according to claim any one of 1-3, it is characterised in that the plain amino of the class mycetocyte Acid, phycobniliprotein, laver amylose are extracted from seaweed to be made.
5. application of the natural radiation-preventing composition according to claim any one of 1-3 in cosmetics are prepared.
6. application according to claim 5, it is characterised in that the cosmetics are emulsion.
7. a kind of emulsion as claimed in claim 6, it is characterised in that the emulsion is consisted of the following composition：
8. a kind of method for preparing emulsion as claimed in claim 7, it is characterised in that the described method comprises the following steps：
1) stearic acid, stearine, stearyl alcohol, octanoic acid/certain herbaceous plants with big flowers acid glycerol three ester, Dormant oils, OXYBENZONE, laruyl alcohol are gathered Ether -20, butyl p-hydroxybenzoate mixing put into oil phase pot heating stirring to 85 degree as A phases, and stirring and dissolving is incubated afterwards completely It is stand-by；
2) glycerine, C12-13 sodium alkyl sulfates, allantoin, ethyl-para-hydroxybenzoate, deionized water are mixed and be added to as B Heating stirring is to 85 degree in aqueous phase pot, heat preservation for standby use after stirring and dissolving is complete；
3) emulsification pot is preheated to 60-65 degree, B phases are first pumped into by 50 revs/min of mixing speed, then A phases are pumped into, stirring 30 Minute, temperature is maintained at 85 degree；
4) C is added to emulsification pot, homogeneous 3 minutes, insulated and stirred 30 minutes, temperature is maintained at 80-85 degree, mixing speed 35 Rev/min；The C phases are hydroxyethyl cellulose；
5) 42 degree of addition D phases are cooled to, insulated and stirred 10 minutes, 25-30 revs/min of mixing speed is eventually adding E phases, is incubated 10-20 minutes；The D phases are composition described in claim 1, and the E phases are butyl p-hydroxybenzoate, P-hydroxybenzoic acid Ethyl ester and essence；
6) stirring is cooled to 40 degree of dischargings.
CN201710212195.XA 2017-03-31 2017-03-31 A kind of natural radiation-preventing composition and its application Withdrawn CN106937918A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108852893A (en) * 2018-08-24 2018-11-23 恩施西特优生态农业开发有限公司 Tea polyphenols facial mask and preparation method thereof
CN110151676A (en) * 2019-05-30 2019-08-23 汕头大学 A kind of sunscreen composition and its application by algae extraction

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108852893A (en) * 2018-08-24 2018-11-23 恩施西特优生态农业开发有限公司 Tea polyphenols facial mask and preparation method thereof
CN110151676A (en) * 2019-05-30 2019-08-23 汕头大学 A kind of sunscreen composition and its application by algae extraction

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