CN107373308A - A kind of method that novel red pigment is produced using pseudo-ginseng slag - Google Patents
A kind of method that novel red pigment is produced using pseudo-ginseng slag Download PDFInfo
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- CN107373308A CN107373308A CN201710559473.9A CN201710559473A CN107373308A CN 107373308 A CN107373308 A CN 107373308A CN 201710559473 A CN201710559473 A CN 201710559473A CN 107373308 A CN107373308 A CN 107373308A
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- 244000131316 Panax pseudoginseng Species 0.000 title claims abstract description 87
- 235000003181 Panax pseudoginseng Nutrition 0.000 title claims abstract description 87
- 239000002893 slag Substances 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 34
- 239000001054 red pigment Substances 0.000 title abstract description 5
- 230000004151 fermentation Effects 0.000 claims abstract description 53
- 238000000855 fermentation Methods 0.000 claims abstract description 52
- 241000228347 Monascus <ascomycete fungus> Species 0.000 claims abstract description 33
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 240000005384 Rhizopus oryzae Species 0.000 claims abstract description 20
- 235000013752 Rhizopus oryzae Nutrition 0.000 claims abstract description 20
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 19
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000010563 solid-state fermentation Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 12
- 241001112078 Aspergillus usamii Species 0.000 claims abstract description 11
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 241000228212 Aspergillus Species 0.000 claims description 29
- 244000113306 Monascus purpureus Species 0.000 claims description 20
- 235000002322 Monascus purpureus Nutrition 0.000 claims description 20
- 229940057059 monascus purpureus Drugs 0.000 claims description 20
- 150000001875 compounds Chemical class 0.000 claims description 19
- 238000004519 manufacturing process Methods 0.000 claims description 15
- 239000002054 inoculum Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 241000030999 Monascus pilosus Species 0.000 claims description 7
- 241000031003 Monascus ruber Species 0.000 claims description 6
- 241000233866 Fungi Species 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 2
- 239000011343 solid material Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 9
- 238000004064 recycling Methods 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract 2
- 206010013786 Dry skin Diseases 0.000 description 7
- 238000000227 grinding Methods 0.000 description 7
- 238000011081 inoculation Methods 0.000 description 7
- 238000007873 sieving Methods 0.000 description 7
- 238000002255 vaccination Methods 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000001360 synchronised effect Effects 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229940066779 peptones Drugs 0.000 description 2
- 229940026314 red yeast rice Drugs 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000012681 biocontrol agent Substances 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000000567 combustion gas Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000000576 food coloring agent Substances 0.000 description 1
- 235000002864 food coloring agent Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229940107131 ginseng root Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000013370 mutualism Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical group 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 235000020097 white wine Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
- A23L5/43—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of method using pseudo-ginseng slag fermenting and producing novel red pigment.What the present invention was realized in：（1）Pseudo-ginseng slag is dried, crushed, sieves pretreatment；（2）Pseudo-ginseng slag, water and nitrogen source by pretreatment is well mixed by a certain percentage；（3）Well mixed material is subjected to steam sterilizing processing；（4）At least one of at least one monascus and aspergillus niger, Aspergillus usamii, Rhizopus oryzae are inoculated with material after sterilization and carries out mixed culture solid state fermentation；（5）Fermentation culture medium just obtains novel red element after drying.Using the method for the invention, pseudo-ginseng slag can be converted into novel red pigment.Pseudo-ginseng slag is carried out recycling by this method, is turned waste into wealth, and not only reduces its pollution on the environment, and and can provides a kind of novel red pigment for food and medicine industry.
Description
Technical field
The invention belongs to trade waste recycling field, is related to a kind of method that haematochrome is produced using pseudo-ginseng slag.
Background technology
With developing rapidly for China's traditional Chinese medicine, the discarded amount of Chinese medicine slag of the major herbal pharmaceutical factory in the whole nation increasingly increases
Add.The processing of Chinese medicine slag often uses traditional processing method, such as burns, the stacking of landfill, FX, this not only needs to put into
Substantial amounts of fund, it can also cause the waste of resource and serious environmental pollution.Therefore, how reasonably to dispose and utilize Chinese medicine slag
It is to realize a unavoidable major issue in modernization of Chinese medicine process.The approach of recycling is carried out to Chinese medicine slag at present
Mainly there is fermentation production of protein feedstuff, used as feed addictive, make organic fertilizer, prepare activated carbon, cracking production combustion gas
Deng.
Pseudo-ginseng is China's tradition rare traditional Chinese medicine, and a kind of medical and edible dual purpose plant, its rhizome can be used as medicine, the master of pseudo-ginseng
It is saponin(e to want Pharmaceutical ingredients, and the pseudo-ginseng slag after effective component extracting is disposed usually as discarded object.After extracted total saposins
Pseudo-ginseng slag in still containing the nutriment such as starch, cellulose, protein, amino acid and various trace elements, as
Fermentation substrate further develops, and can turn waste into wealth, and improves the utilization ratio of resource.Application No. 201010232573.9
Chinese invention patent disclose " it is a kind of with fermenting and distilling pseudo-ginseng root dregs prepare white wine and method "；Application No.
201110119291.2 Chinese invention patent discloses " a kind of method with pseudo-ginseng slag fermentation production of protein feedstuff "；Application number
A kind of " side using pseudo-ginseng slag fermenting and producing trichoderma bio-control agent is disclosed for 201210196021.6 Chinese invention patent
Method "；The Chinese invention patent of Application No. 201210521383.8 discloses " a kind of feeding compound using pseudo-ginseng slag fermenting and producing
The method of enzyme preparation "；The Chinese invention patent of Application No. 201310437721.4 discloses " a kind of to mix bacterium hair using pseudo-ginseng slag
The method that ferment produces medicinal fungal substance "；The Chinese invention patent of Application No. 201310437623.0 discloses " a kind of to utilize pseudo-ginseng
The method that slag prepares red yeast rice mycoplasma ".
Food coloring is divided into two kinds of natural pigment and synthetic food color, because synthetic dyestuff has safety issue, mesh
Preceding people more and more select natural pigment to be used as food additives.Haematochrome is a kind of natural pigment, from red
The fermentating metabolism process of aspergillus, is widely used in food industry, is such as added to meat products, dairy produce, aquatic products, fruit, biscuit
It is the good substitute of chemical synthesis pigment, market prospects are very wide etc. in various kinds of foods.The production of haematochrome can use
Two kinds of techniques of liquid state fermentation and solid state fermentation, comparatively, the yield of solid state fermentation is more higher.Produced currently used for solid state fermentation
The raw material of haematochrome mainly has the cereal crops such as rice, sorghum, corn and soybean, causes production cost higher, and be also consumed by
A large amount of grains.The recycling of pseudo-ginseng slag can be realized by carrying out solid state fermentation production haematochrome as fermentation substrate using pseudo-ginseng slag,
Natural red colouring matter product is obtained, reduces production cost.
Fermented and cultured is using subject matter existing for solid state fermentation of monascus abandoned biomass production haematochrome at present
The color value of haematochrome is relatively low in thing, by taking pseudo-ginseng slag as an example, is fermented using monascus single bacterium, haematochrome color value in its fermentation culture medium
Only 8.0 ~ 16.8u/g.In order to improve haematochrome color value in fermentation culture medium, the present invention uses aspergillus niger（Aspergillus niger3.1858）, Aspergillus usamii（Aspergillus usamii3.2923）, Rhizopus oryzae（Rhizopus oryzae
3.5842）In one kind and red monascus（Monascus ruber 3.549）, monascus purpureus（Monascus purpureus
3.4577）, M.pilosus（Monascus pilosus3.976）In a kind of carry out mixed fungus fermentation.Due to aspergillus niger, space
Help U.S. aspergillus, Rhizopus oryzae has stronger saccharification capability, and have good mutualism relation with monascus, in mixed fungus fermentation
During can promote the generation of haematochrome, and then significantly improve haematochrome color value in fermentation culture medium：Mixed fungus fermentation culture
Middle haematochrome color value is generally up to 42.6 ~ 58.3u/g.
The content of the invention
It is an object of the invention to provide a kind of method that haematochrome is produced using pseudo-ginseng slag solid state fermentation.It is of the present invention
It is as follows successively using the method for pseudo-ginseng slag solid state fermentation production haematochrome, its processing step：
1st, the pretreatment of pseudo-ginseng slag
It will be pulverized and sieved after wet pseudo-ginseng slag drying, select particle diameter 0.2-0.4mm pseudo-ginseng slag to be used for subsequent technique；
2nd, dispensing and batch mixing
Pseudo-ginseng slag, appropriate nitrogen source and water by pretreatment is well mixed, specific batching mode is as follows：
The mass ratio of pretreated 100 parts of pseudo-ginseng slag, nitrogen source 4-6 parts, solid material and water is 1:1.8-1:2.5；
First nitrogen source is added to the water during dispensing well mixed, then the solution prepared is well mixed with pseudo-ginseng slag；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Compound after sterilization treatment is subjected to mixed fungus fermentation, strain is inoculated with by 10%-20% inoculum concentration, fermentation condition：
Pressure is normal pressure, and temperature is 28 DEG C -30 DEG C, and fermentation period is -14 days 10 days；
5th, dry
Fermentation culture medium is just turned into haematochrome product after 60 DEG C of drying.
In methods described, during dispensing nitrogen source used be dusty yeast, the one or more in urea, peptone, ammonium sulfate；
In methods described, strain used comprises at least aspergillus niger during fermentation（Aspergillus niger3.1858）, space assistant
U.S. aspergillus（Aspergillus usamii3.2923）, Rhizopus oryzae（Rhizopus oryzae 3.5842）In one kind and
Red monascus（Monascus ruber 3.549）, monascus purpureus（Monascus purpureus3.4577）, feathering red yeast rice
It is mould（Monascus pilosus3.976）In one kind；
The invention has the advantages that：
（1）Using the method for the invention, pseudo-ginseng slag is converted into haematochrome, it is possible to achieve the recycling of pseudo-ginseng slag, drop
The production cost of low haematochrome.
（2）The red produced by haematochrome prepared by the method for the invention with existing monascus single bacterium zymotechnique
Element is compared, and color value has significant raising.
（3）Compared with liquid state fermentation, the solid-state fermentation process that uses of the present invention has that production equipment is simple, energy consumption is low, production
Raw waste residue, the advantage such as waste water is few, environmental pollution is small, contribute to manufacturing enterprise to realize energy-saving and emission-reduction and clean manufacturing.
Embodiment
The present embodiment of embodiment 1 is using pseudo-ginseng slag as raw material, red monascus（Monascusruber3.549）/ black song
It is mould（Aspergillus niger3.1858）Mixed culture solid state fermentation, which is carried out, for strain produces haematochrome.Comprise the following steps that：
1st, the pretreatment of pseudo-ginseng slag
Pseudo-ginseng slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with the pseudo-ginseng dregs of a decoction in easy crushing state.Will
Dried pseudo-ginseng slag grinding and sieving, particle diameter 0.2-0.3mm pseudo-ginseng slag is selected to be used for subsequent technique；
2nd, dispensing and batch mixing
First 4g dusty yeasts are added in 250g water, are well mixed, then again by the solution and the 100g pseudo-ginseng by pretreatment
Ground-slag is well mixed；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Fermented using synchronous vaccination ways, red monascus is accessed in the compound after sterilization treatment
（Monascusruber3.549）And aspergillus niger（Aspergillus niger3.1858）Fermented, red monascus
（Monascusruber3.549）And aspergillus niger（Aspergillus niger3.1858）Inoculation compare for 1:1, total inoculum concentration
For 15%, fermentation condition：Fermentation period is 14 days, and pressure is normal pressure, and temperature is 30 DEG C；
5th, dry
Product after fermentation just obtains haematochrome product through baking oven after normal pressure, 60 DEG C of dryings, and haematochrome color value is in the product
58.3u/g。
The present embodiment of embodiment 2 is using pseudo-ginseng slag as raw material, monascus purpureus（Monascus purpureus3.4577）/
Rhizopus oryzae（Rhizopus oryzae 3.5842）Mixed culture solid state fermentation, which is carried out, for strain produces haematochrome.Comprise the following steps that：
1st, the pretreatment of pseudo-ginseng slag
Pseudo-ginseng slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with the pseudo-ginseng dregs of a decoction in easy crushing state.Will
Dried pseudo-ginseng slag grinding and sieving, particle diameter 0.3-0.4mm pseudo-ginseng slag is selected to be used for subsequent technique；
2nd, dispensing and batch mixing
First 6g ammonium sulfate is added in 180g water, is well mixed, then again by the solution and the 100g pseudo-ginseng by pretreatment
Ground-slag is well mixed；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Fermented using asynchronous vaccination ways, first access Rhizopus oryzae in the compound after sterilization treatment（Rhizopus oryzae 3.5842）, then monascus purpureus is accessed again after fermenting 48 hours（Monascus purpureus3.4577）Enter
Row fermentation, Rhizopus oryzae（Rhizopus oryzae 3.5842）And monascus purpureus（Monascus purpureus3.4577）'s
Inoculation is than being 1:1, total inoculum concentration is 10%, fermentation condition：Fermentation period is 10 days, and pressure is normal pressure, and 2 days temperature are before fermentation
28 DEG C, until fermentation ends since fermenting the 3rd day, temperature is 30 DEG C；
5th, dry
Product after fermentation just obtains haematochrome product through baking oven after normal pressure, 60 DEG C of dryings, and haematochrome color value is in the product
42.6u/g。
The present embodiment of embodiment 3 is using pseudo-ginseng slag as raw material, M.pilosus（Monascuspilosus3. 976）/ space
Help U.S. aspergillus（Aspergillus usamii3.2923）Mixed culture solid state fermentation, which is carried out, for strain produces haematochrome.Specific steps
It is as follows：
1st, the pretreatment of pseudo-ginseng slag
Pseudo-ginseng slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with the pseudo-ginseng dregs of a decoction in easy crushing state.Will
Dried pseudo-ginseng slag grinding and sieving, particle diameter 0.2-0.4mm pseudo-ginseng slag is selected to be used for subsequent technique；
2nd, dispensing and batch mixing
First 6g peptones are added in 265g water, are well mixed, then again by the solution and the 100g pseudo-ginseng by pretreatment
Ground-slag is well mixed；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Fermented using synchronous vaccination ways, M.pilosus is synchronously accessed in the compound after sterilization treatment
（Monascuspilosus3. 976）And Aspergillus usamii（Aspergillus usamii3.2923）Fermented, feathering is red
Aspergillus（Monascuspilosus3. 976）And Aspergillus usamii（Aspergillus usamii3.2923）Inoculation compare and be
1:1, total inoculum concentration is 20%, fermentation condition：Fermentation period is 12 days, and pressure is normal pressure, and fermentation temperature is 29 DEG C；
5th, dry
Product after fermentation just obtains haematochrome product through baking oven after normal pressure, 60 DEG C of dryings, and haematochrome color value is in the product
57.8u/g。
The present embodiment of embodiment 4 is using pseudo-ginseng slag as raw material, monascus purpureus（Monascus purpureus3.4577）/
Aspergillus niger（Aspergillus niger3.1858）Mixed culture solid state fermentation, which is carried out, for strain produces haematochrome.Specific steps are such as
Under：
1st, the pretreatment of pseudo-ginseng slag
Pseudo-ginseng slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with the pseudo-ginseng dregs of a decoction in easy crushing state.Will
Dried pseudo-ginseng slag grinding and sieving, particle diameter 0.2-0.3mm pseudo-ginseng slag is selected to be used for subsequent technique；
2nd, dispensing and batch mixing
First 4g ammonium sulfate is added in 240g water, is well mixed, then again by the solution and the 100g pseudo-ginseng by pretreatment
Ground-slag is well mixed；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Fermented using synchronous vaccination ways, monascus purpureus is accessed in the compound after sterilization treatment（Monascus purpureus3.4577）And aspergillus niger（Aspergillus niger3.1858）Fermented, monascus purpureus（Monascus purpureus3.4577）And aspergillus niger（Aspergillus niger3.1858）Inoculation compare for 1:1, total inoculum concentration is
20%, fermentation condition：Fermentation period is 12 days, and pressure is normal pressure, and temperature is 30 DEG C；
5th, dry
Product after fermentation just obtains haematochrome product through baking oven after normal pressure, 60 DEG C of dryings, and haematochrome color value is in the product
57.1u/g。
The present embodiment of embodiment 5 is using pseudo-ginseng slag as raw material, monascus purpureus（Monascus purpureus3.4577）/
Aspergillus usamii（Aspergillus usamii3.2923）Mixed culture solid state fermentation, which is carried out, for strain produces haematochrome.Specific step
It is rapid as follows：
1st, the pretreatment of pseudo-ginseng slag
Pseudo-ginseng slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with the pseudo-ginseng dregs of a decoction in easy crushing state.Will
Dried pseudo-ginseng slag grinding and sieving, particle diameter 0.3-0.4mm pseudo-ginseng slag is selected to be used for subsequent technique；
2nd, dispensing and batch mixing
First 6g urea is added in 255g water, is well mixed, then again by the solution and the 100g pseudo-ginseng slags by pretreatment
Powder is well mixed；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Fermented using asynchronous vaccination ways, first access Aspergillus usamii in the compound after sterilization treatment
（Aspergillus usamii3.2923）, then monascus purpureus is accessed again after fermenting 24 hours（Monascus purpureus3.4577）Fermented, Aspergillus usamii（Aspergillus usamii3.2923）And monascus purpureus
（Monascus purpureus3.4577）Inoculation compare for 1:1, total inoculum concentration is 10%, fermentation condition：Fermentation period is 14
My god, pressure is normal pressure, and fermentation temperature is 28 DEG C；
5th, dry
Product after fermentation just obtains haematochrome product through baking oven after normal pressure, 60 DEG C of dryings, and haematochrome color value is in the product
55.7u/g。
The present embodiment of embodiment 6 is using pseudo-ginseng slag as raw material, monascus purpureus（Monascus purpureus3.4577）/
M.pilosus（Monascuspilosus3. 976）/ Aspergillus usamii（Aspergillus usamii3.2923）/ black song
It is mould（Aspergillus niger3.1858）Mixed culture solid state fermentation, which is carried out, for strain produces haematochrome.Comprise the following steps that：
1st, the pretreatment of pseudo-ginseng slag
Pseudo-ginseng slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with the pseudo-ginseng dregs of a decoction in easy crushing state.Will
Dried pseudo-ginseng slag grinding and sieving, particle diameter 0.3-0.4mm pseudo-ginseng slag is selected to be used for subsequent technique；
2nd, dispensing and batch mixing
First 6g dusty yeasts are added in 255g water, are well mixed, then again by the solution and the 100g pseudo-ginseng by pretreatment
Ground-slag is well mixed；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Fermented using asynchronous vaccination ways, first access Aspergillus usamii in the compound after sterilization treatment
（Aspergillus usamii3.2923）And aspergillus niger（Aspergillus niger3.1858）, it is then small in fermentation 24
Shi Houzai accesses monascus purpureus（Monascus purpureus3.4577）And M.pilosus（Monascuspilosus3.
976）Fermented, monascus purpureus（Monascus purpureus3.4577）, M.pilosus（Monascuspilosus3.
976）, Aspergillus usamii（Aspergillus usamii3.2923）, aspergillus niger（Aspergillus niger3.1858）'s
Inoculation is than being 1:1:1:1, total inoculum concentration is 20%, fermentation condition：Fermentation period is 12 days, and pressure is normal pressure, fermentation temperature 28
℃；
5th, dry
Product after fermentation just obtains haematochrome product through baking oven after normal pressure, 60 DEG C of dryings, and haematochrome color value is in the product
54.8u/g。
The present embodiment of embodiment 7 is using pseudo-ginseng slag as raw material, monascus purpureus（Monascus purpureus3.4577）/
Red monascus（Monascus ruber 3.549）/ Rhizopus oryzae（Rhizopus oryzae 3.5842）Mixed bacterium is carried out for strain
Solid state fermentation produces haematochrome.Comprise the following steps that：
1st, the pretreatment of pseudo-ginseng slag
Pseudo-ginseng slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with the pseudo-ginseng dregs of a decoction in easy crushing state.Will
Dried pseudo-ginseng slag grinding and sieving, particle diameter 0.3-0.4mm pseudo-ginseng slag is selected to be used for subsequent technique；
2nd, dispensing and batch mixing
First 5g peptones are added in 250g water, are well mixed, then again by the solution and the 100g pseudo-ginseng by pretreatment
Ground-slag is well mixed；
3rd, sterilize
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
4th, ferment
Fermented using asynchronous vaccination ways, Rhizopus oryzae is synchronously accessed in the compound after sterilization treatment（Rhizopus oryzae 3.5842）, monascus purpureus（Monascus purpureus3.4577）, red monascus（Monascus ruber
3.549）Fermented, Rhizopus oryzae（Rhizopus oryzae 3.5842）, monascus purpureus（Monascus purpureus
3.4577）, red monascus（Monascus ruber 3.549）Inoculation compare for 1:1:1, total inoculum concentration is 15%, and ferment bar
Part：Fermentation period is 13 days, and pressure is normal pressure, and fermentation temperature is 29 DEG C；
5th, dry
Product after fermentation just obtains haematochrome product through baking oven after normal pressure, 60 DEG C of dryings, and haematochrome color value is in the product
57.6u/g。
Claims (4)
1. one kind passes through the method for mixed culture solid state fermentation technique productions haematochrome, its concrete technology feature using pseudo-ginseng slag as raw material
It is as follows：
（1）The pretreatment of pseudo-ginseng slag
It will be pulverized and sieved after wet pseudo-ginseng slag drying, select particle diameter 0.2-0.4mm pseudo-ginseng slag to be used for subsequent technique；
（2）Dispensing and batch mixing
Pseudo-ginseng slag, appropriate nitrogen source and water by pretreatment is well mixed, specific batching mode is as follows：
The mass ratio of pretreated 100 parts of pseudo-ginseng slag, nitrogen source 4-6 parts, solid material and water is 1:1.8-1:2.5；
First nitrogen source is added to the water during dispensing well mixed, then the solution prepared is well mixed with pseudo-ginseng slag；
（3）Sterilizing
Compound is subjected to sterilization treatment using autoclaving：Steam pressure 1atm, 121 DEG C of temperature, the time is at least
30 minutes；
（4）Fermentation
Compound after sterilization treatment is subjected to mixed fungus fermentation, strain is inoculated with by 10%-20% inoculum concentration, fermentation condition：
Pressure is normal pressure, and temperature is 28 DEG C -30 DEG C, and fermentation period is -14 days 10 days；
（5）Dry
Fermentation culture medium is just turned into haematochrome product after 60 DEG C of drying.
2. according to the method for claim 1, it is characterised in that during dispensing nitrogen source used be dusty yeast, urea, peptone,
One or more in ammonium sulfate.
3. according to the method for claim 1, it is characterised in that the strain used during fermentation comprises at least aspergillus niger
（Aspergillus niger3.1858）, Aspergillus usamii（Aspergillus usamii3.2923）, Rhizopus oryzae
（Rhizopus oryzae 3.5842）In one kind and red monascus（Monascus ruber 3.549）, monascus purpureus
（Monascus purpureus3.4577）, M.pilosus（Monascus pilosus3.976）In one kind.
4. the haematochrome that a kind of one of claim 1-3 methods described obtains.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108936705A (en) * | 2018-07-30 | 2018-12-07 | 金华职业技术学院 | Emblic intensive processing method and emblic seeder |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899378A (en) * | 2012-10-18 | 2013-01-30 | 昆明理工大学 | Method for biotransformation of Panax notoginseng (Burk.) F. H. Chen medicinal material by using Monascus purpureus |
| CN103493682A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing lucid ganoderma pseudo-ginseng mycoplasm by fermenting pseudo-ginseng dregs |
| CN103497973A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing red rice pseudo-ginseng mycoplasma by using pseudo-ginseng residue fermentation |
| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
-
2017
- 2017-07-11 CN CN201710559473.9A patent/CN107373308B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899378A (en) * | 2012-10-18 | 2013-01-30 | 昆明理工大学 | Method for biotransformation of Panax notoginseng (Burk.) F. H. Chen medicinal material by using Monascus purpureus |
| CN103493682A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing lucid ganoderma pseudo-ginseng mycoplasm by fermenting pseudo-ginseng dregs |
| CN103497973A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing red rice pseudo-ginseng mycoplasma by using pseudo-ginseng residue fermentation |
| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
Non-Patent Citations (3)
| Title |
|---|
| 庄桂: ""发酵麸皮制取红曲色素的初步研究"", 《中国酿造》 * |
| 田园: ""微生物和铵盐对红曲色素代谢合成的影响及其机理的初步研究"", 《中国优秀硕士学位论文全文数据库.工程科技Ⅰ辑》 * |
| 谭显东: ""培养基特性对三七渣发酵产红曲色素的影响"", 《环境工程学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108936705A (en) * | 2018-07-30 | 2018-12-07 | 金华职业技术学院 | Emblic intensive processing method and emblic seeder |
| CN108936705B (en) * | 2018-07-30 | 2021-06-04 | 金华职业技术学院 | Deep processing method of phyllanthus emblica and phyllanthus emblica kernel removing device |
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