CN107320392B - Hand sanitizer and preparation method thereof - Google Patents

Abstract

The invention discloses a hand sanitizer and a preparation method thereof, wherein the hand sanitizer comprises the following components in percentage by mass: 5-15% of sodium fatty alcohol-polyoxyethylene ether sulfate, 5-15% of sodium dodecyl benzene sulfonate, 1.5-3.5% of coconut oil fatty acid diethanolamide, 3.0-6.0% of glycerol, 1.0-2.0% of sodium chloride, 0.2-0.5% of sodium benzoate, 0.2-1.0% of EDTA-2Na, 0.5-2.0% of cortex moutan extract, 1.0-1.5% of ethanol and the balance of distilled water. The hand sanitizer prepared by the invention has low alkalinity, no stimulation, itching relieving and pain relieving effects, can relieve itching feeling during allergy, mosquito and insect bite and eczema type skin cleaning, and has good decontamination effect. The operation is simple and the cost is low.

Description

Hand sanitizer and preparation method thereof

Technical Field

The invention relates to the technical field of washing products, and particularly relates to a hand sanitizer and a preparation method thereof.

Background

Eczema is a skin inflammatory reaction with intense pruritus caused by various internal and external factors. It is divided into three stages of acute stage, subacute stage and chronic stage. The acute stage is prone to exudation, while the chronic stage is infiltrative and hypertrophic. Eczema generally has long duration of disease course, and pruritus is difficult to endure particularly when bathing, washing hands and the like are stimulated by cold and heat. Therefore, the hand sanitizer which has no stimulation, obvious itching and pain relieving and involuntary scratching prevention is necessary to be found for the eczema skin.

The cortex moutan is dry root bark of a Ranunculaceae plant peony, is a Chinese medicinal material with high medicinal value, and has the effects of clearing heat, cooling blood, promoting blood circulation, removing blood stasis and the like. Paeonol and flavone are effective components in cortex moutan. Wherein paeonol has antiinflammatory, itching relieving, and antiallergic effects. The existing paeonol ointment has the effects of resisting allergy, diminishing inflammation and relieving itching, and is clinically used for various skin diseases such as eczema, dermatitis, pruritus, mosquito and bedbug bite redness and swelling and the like. [ Wuhaijun, Xusuihui, et al, anti-inflammatory action of paeonol, proceedings of Baotou medical college, 2008,24(3): 238-; [ Shilian Hu, Gan Shen. Paeonol, the mian active principles of paenoia moutan, ameliorants, alcoholic stephanitis peptides. journal of Ethnopharmacology.2012,108: 100. times. 106], demonstrated that paeonol has significant swelling-inhibiting, i.e., anti-inflammatory, effects on rats with swollen footpad. In addition, the Chinese medicines include (lotus, tanghui, Zhang Yongjun, Zhang Yi Jun, Typha, Lilianjiang, Deng Fengmei, and Nitraria tangutorum, the repairing effect of Nitraria tangutorum on endothelial cells damaged by high sugar, the pharmacology and clinic of the Chinese medicines, 2010,26 (4): 21-24], demonstrating that flavone has the functions of repairing the loss of cell endothelium, relieving pain, preventing inflammatory enzyme from exudation, accelerating wound healing and the like.

In the conventional extraction method of the cortex moutan extract, chloroform and carbon tetrachloride are used in an organic solvent extraction method, so that the cortex moutan extract is high in toxicity, heavy in pollution and not suitable for industrial production; in the alcohol extraction method, although ethanol is less toxic, the extraction efficiency using ethanol is slightly low, and even if a method of extracting more than several times is adopted, the amount of extracted impurities may increase accordingly.

Therefore, aiming at the trouble of washing and protecting the allergic and eczematous skin, the development of the hand sanitizer which has no stimulation to the skin, has obvious itching relieving and pain relieving effects on the allergic, mosquito biting and eczematous skin and prevents involuntary scratching is urgently needed.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide the hand sanitizer, which solves the problems of itching relieving and pain relieving of the hand sanitizer without the special hand sanitizer for eczema type and allergic type skins at present, and particularly solves the problems of itching relieving and pain relieving of the hand sanitizer through compatibility of cortex moutan extracts and the like.

The invention also provides an extraction method of the cortex moutan extract, which solves the problems of low recovery rate of extracted active ingredients, environmental pollution and more impurities.

In order to achieve the purpose, the invention adopts the following technical scheme: the hand sanitizer is characterized by comprising the following components in percentage by mass: 5-15% of sodium fatty alcohol-polyoxyethylene ether sulfate, 5-15% of sodium dodecyl benzene sulfonate, 1.5-3.5% of coconut oil fatty acid diethanolamide, 3.0-6.0% of glycerol, 1.0-2.0% of sodium chloride, 0.2-0.5% of sodium benzoate, 0.2-1.0% of EDTA-2Na, 0.5-2.0% of cortex moutan extract, 1.0-1.5% of ethanol and the balance of distilled water.

Further, the cortex moutan extract is prepared by a double aqueous phase method, and comprises the following steps:

1) crushing cortex moutan, sieving with a pharmacopeia sieve No. 4 to obtain cortex moutan fine powder, adding the cortex moutan fine powder into an ethanol-water-sodium chloride aqueous phase serving as an extraction solvent, soaking at room temperature for 10-28 h, oscillating and extracting at the temperature of 25-50 ℃ for 2-4 h, standing for 1-2 h, and filtering to remove dregs to obtain crude extract of the cortex moutan;

2) standing the crude cortex moutan extract prepared in the step 1) at a room temperature for 1-24 h, separating the upper phase from the lower phase, removing the lower phase, and distilling the upper phase under reduced pressure to remove the solvent to obtain the cortex moutan extract.

Further, the concentration of a sodium chloride aqueous solution in the ethanol-water-sodium chloride double aqueous phase is 8-20%, the volume ratio of the ethanol to the sodium chloride aqueous solution is 0.25-2: 1, and the volume mass ratio of the double aqueous phase to the cortex moutan fine powder is 10 mL: 1.5 g-10 mL: 0.4 g.

Further, the preparation method of the hand sanitizer is characterized by preparing the materials according to the proportion, and comprises the following steps:

1) weighing fatty alcohol-polyoxyethylene ether sodium sulfate, sodium dodecyl benzene sulfonate, sodium benzoate, EDTA-2Na and sodium chloride, pouring into a clean beaker, adding distilled water, placing into a constant-temperature water bath kettle at 25-50 ℃, and continuously stirring to completely dissolve the added components;

2) adding coconut oil fatty acid diethanolamide and glycerol into the solution obtained in the step 1), and continuously stirring to uniformly mix the coconut oil fatty acid diethanolamide and the glycerol to obtain a basic hand sanitizer;

3) weighing cortex moutan extract, adding ethanol, stirring and dissolving; slowly adding the cortex moutan extract solution into the basic hand sanitizer prepared in the step 2) under continuous stirring, and uniformly stirring to obtain the hand sanitizer.

Compared with the prior art, the invention has the following beneficial effects:

1. the hand sanitizer adopts two anionic surfactants of fatty alcohol-polyoxyethylene ether sodium sulfate and sodium dodecyl benzene sulfonate and a nonionic surfactant of coconut oil fatty acid diethanolamide as cleaning components, wherein the fatty alcohol-polyoxyethylene ether sodium sulfate, the sodium dodecyl benzene sulfonate and the coconut oil fatty acid diethanolamide have excellent functions of decontamination, emulsification and wetting, and are good in solubility and thickening effect, and the compound of the fatty alcohol-polyoxyethylene ether sodium sulfate, the sodium dodecyl benzene sulfonate and the coconut oil fatty acid diethanolamide can reduce the dosage of the surfactant, reduce the cost and improve the use efficiency. When the sodium fatty alcohol polyoxyethylene ether sulfate: sodium dodecylbenzenesulfonate: the coconut oil fatty acid diethanolamide has the best effect when the ratio is 5:5: 1; glycerin is also added to provide a moisturizing component; also contains antipruritic and analgesic components such as paeonol and flavone. Is slightly alkaline, is similar to the pH value of a human body, and has no stimulation to the skin of the human body. In addition, the appearance, the surface active substance content, the solid content, the pH value and the foam height of the hand sanitizer formula all meet the quality standard requirements of the hand sanitizer. The cleaning liquid has no stimulation during trial, has the effects of relieving itching and relieving pain, is suitable for cleaning before nursing of allergic and eczema type skin, relieves the pain of the allergic, mosquito and insect bites and the eczema type skin during cleaning, and prevents the itching and scratching damage in the cleaning process.

2. In the preparation process of the hand sanitizer, two anionic surfactants (fatty alcohol-polyoxyethylene ether sodium sulfate and sodium dodecyl benzene sulfonate) and a nonionic surfactant (coconut oil fatty acid diethanolamide) are added as cleaning components of the hand sanitizer, wherein the prepared raw materials of the fatty alcohol-polyoxyethylene ether sodium sulfate, the sodium dodecyl benzene sulfonate and the coconut oil fatty acid diethanolamide are rich, the detergency is good, and the price is low. Other components such as water-resistant hardness component, moisture-keeping component and bacteriostatic component are cheap and easily available, and the cost is low. The hand sanitizer prepared by the invention has the characteristics of simple instrument and equipment and simple operation.

3. The invention adopts a double aqueous phase method to extract effective components including paeonol and flavone from the dried root bark of peony, and the method is simple and novel. The lower phase of the aqueous two phases is mainly water and sodium chloride components, and compared with pure water or organic solvent extraction, theoretically, in the process of soaking and extracting the medicinal materials in saline water, histiocytes are easy to swell and break, effective components are easy to dissolve out, mass transfer is fast, and compared with an alcohol extraction method, the recovery rate and the purity of the extracted effective components are high. The extraction and liquid-liquid distribution are combined in the process of the double aqueous phase method, the two phases are static and layered after extraction, the extracted paeonol and flavone components are distributed on the upper phase and are preliminarily separated from other inorganic components, saccharides, amino acids and other strong polar impurities distributed on the lower phase in medicinal materials, the content of target effective components in the extract is high, the condition that the hand sanitizer contains saccharides, amino acids and other nutrients, microorganisms are easy to breed, the hand sanitizer is deteriorated, and the quality guarantee period is shortened. The aqueous two phases reduce the dosage of organic solvent, the separated lower phase solution can be repeatedly used, the raw materials are cheap and easily available, and the extraction cost is reduced.

Detailed Description

The present invention will be described in further detail with reference to specific examples.

Extraction method and content determination of cortex moutan extract

1) Preparing cortex moutan extract

Example (two aqueous phase process): 50.0mL of 15% sodium chloride solution and 30.0mL of ethanol are added into a container with a plug respectively, and the mixture is kept stand for demixing to form a double water phase. The volume of the upper and lower phases in each tube was recorded. Weighing appropriate amount of cortex moutan, pulverizing with a pulverizer, sieving with pharmacopeia No. 4 sieve, and making into cortex moutan fine powder. 9.832g of fine powder of cortex moutan is added into the above aqueous phase, and the mixture is left standing at room temperature for 24 hours and shaken at constant temperature of 37 ℃ for 2 hours. Standing for 2 hr, filtering, and removing residue. After standing for 2 hours, the upper and lower phases were separated. Distilling the upper phase solution under reduced pressure to remove solvent to obtain cortex moutan extract, weighing 0.722g, wherein the extract accounts for 7.34% of the weight of the medicinal materials.

Comparative example (alcohol extraction): building a reflux device, precisely weighing 25.02g of cortex moutan powder in a 250ml round-bottom flask, adding 180ml of ethanol, and performing reflux extraction for 5 h. Filtering, and removing residue. The extract was placed on a rotary evaporator to remove ethanol, transferred to a vial of penicillin and weighed to a mass of 2.41 g. The extract is a brown viscous paste in appearance, and is difficult to completely dry, and has many impurities, especially high sugar content (sugar is easy to absorb water).

2) Determination of paeonol content and flavone content in cortex moutan extract

The content of paeonol is determined by HPLC. Chromatographic conditions are as follows: stationary phase C₁₈Column, mobile phase 80% methanol, flow rate 0.8mL/min, detection wavelength 230 nm. The extract of 1) was measured by the standard curve method.

The content of paeonol in cortex moutan extract obtained by two aqueous phase method is 88.7 mg. Calculated, the one-time extraction of the paeonol accounts for 0.90 percent of the total weight of the cortex moutan medicinal materials, and the content of the paeonol in the extract is 12.3 percent.

The content of paeonol in the extract obtained by the alcohol extraction method is 0.206g, and the calculated primary extraction of the paeonol accounts for 0.82 percent of the total weight of the cortex moutan medicinal materials, and the content of the paeonol in the extract is 8.55 percent.

The content of flavone is measured by adopting an ultraviolet-visible spectrophotometry: transferring 150 μ L of flavone extractive solution into 10mL volumetric flask with micropipette, adding 0.3mL of 5% sodium nitrite solution into volumetric flask, adding 0.3mL of 10% aluminum nitrate solution after 5 minutes, and adding 4mL of 1mol L of aluminum nitrate solution after 5 minutes^-1And (3) adding a sodium hydroxide solution into a volume fixed to a scale by using a 70% ethanol solution, taking a solution without a sample as a blank after 15 minutes, and measuring the absorbance of the solution at 510nm by using an ultraviolet spectrophotometry. Rutin is used as a reference substance and is used as a standard curve to measure that the content of the flavone in the cortex moutan extract obtained in the embodiment is 290.6 mg. Calculating to obtain flavone zhuandan2.96% of the total weight of the bark medicinal material and 40.2% of flavone in the extract.

In conclusion, compared with the alcohol extraction method, the aqueous two-phase method has the advantages of high recovery rate of the extracted effective components, high purity, difficult water absorption and high stability.

Second, preparation method of hand sanitizer

Example 1

1) 10.003g of sodium fatty alcohol polyoxyethylene ether sulfate, 10.024g of sodium dodecyl benzene sulfonate, 0.2341g of sodium benzoate, 0.442g of EDTA-2Na and 1.350g of sodium chloride are weighed respectively, poured into a 200mL clean beaker, and 50g of distilled water is added. Placing in a 50 deg.C constant temperature water bath kettle, and stirring to dissolve the components completely.

2) Adding 2.007g of coconut diethanolamide and 2.063g of glycerol into the solution obtained in the step 1), and continuously stirring in a constant-temperature water bath kettle at 50 ℃ to uniformly mix the mixture to obtain the basic hand sanitizer.

3) 0.722g of cortex moutan extract is weighed, 1.2g of ethanol is added, and stirring is carried out. Slowly adding the solution into the basic hand sanitizer prepared in the step 2) under continuous stirring, and uniformly stirring to obtain the hand sanitizer containing the cortex moutan active ingredient.

Examples 2 to 5

The method for preparing the hand sanitizer is the same as example 1, and the contents of the main components are shown in table 1.

TABLE 1 content of main ingredients of hand-washing liquids of different formulations

Example 6

The different hand lotions prepared in examples 1 to 5 were subjected to quality tests.

1. The appearance of the hand sanitizer is yellow viscous semitransparent liquid. And after the refrigerator is placed for 24 hours, no precipitate exists in the appearance, and no layering exists.

2. Respectively weighing 10.00g of the hand sanitizer by using a balance, placing the hand sanitizer in a 100mL volumetric flask, adding distilled water to a constant volume, and uniformly mixing. The pH values of the different hand sanitizer solutions were measured separately with a pH tester, see table 2.

3. 0.132g of each hand sanitizer was weighed into a 50mL beaker and evenly spread on the bottom. Then it was put into an oven and dried at 105 ℃ for 3 hours. And opening the oven, taking out the beaker, weighing the mass of the residual solid in the beaker by using a balance after the beaker is cooled to room temperature, and respectively calculating the solid content, which is shown in table 2.

4. 0.503g of the hand sanitizer is weighed and placed in a 500mL beaker, and 500mL of distilled water is added to prepare the hand sanitizer solution with the mass-volume ratio of 1/1000. Respectively putting 80mL of the hand sanitizer solution into a 200mL beaker, adding 20mL of tap water, uniformly mixing, adding 50mL of the solution into a 100mL measuring cylinder, plugging the opening of the measuring cylinder, shaking up and down for three times, and respectively calculating the foam heights of different hand sanitizer solutions, which is specifically shown in Table 2.

5. Different hand washing liquids are respectively used for cleaning 5 healthy human skins, and the skin after cleaning is smooth and has no stimulation. The decontamination effect is good.

Different hand washing liquids are respectively used for cleaning 3 kinds of skin bitten by mosquitoes and insects and 2 kinds of eczema type skin, and the specific effects are shown in table 2.

TABLE 2 quality testing of hand sanitizers of different formulations

As shown in Table 1, the hand sanitizer prepared by adding the cortex moutan extract has the effects of relieving itching and pain, wherein after the hand sanitizer is used by people suffering from mosquito bites and insect bites, itching and pain are avoided; after the eczema type skin is used, the itching relieving and pain relieving effects last for 10-30 minutes. And the appearance, the surface active substance content, the solid content, the pH and the foam height of the formula of the hand sanitizer all meet the quality standard requirements of the hand sanitizer. The detergent has no stimulation in alkalescence trial and good decontamination effect. The hand sanitizer prepared by the cortex moutan extract is not added, so that the hand sanitizer has no effects of relieving itching and pain on undamaged skin; for damaged skin, due to the difference between the washing water temperature and the skin temperature, the loss of grease on the surface of the skin after washing or the stimulation of a surfactant, the itching and the pain in washing are aggravated, so the cleaning agent is suitable for cleaning before nursing of allergic, mosquito and insect bites and eczema type skin, and can relieve the pain of the allergic, mosquito and insect bites and the eczema type skin during washing.

Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.

Claims (3)

1. The hand sanitizer is characterized by comprising the following components in percentage by mass: 5-15% of sodium fatty alcohol-polyoxyethylene ether sulfate, 5-15% of sodium dodecyl benzene sulfonate, 1.5-3.5% of coconut oil fatty acid diethanolamide, 3.0-6.0% of glycerol, 1.0-2.0% of sodium chloride, 0.2-0.5% of sodium benzoate, 0.2-1.0% of EDTA-2Na, 0.5-2.0% of cortex moutan extract, 1.0-1.5% of ethanol and the balance of distilled water;

the cortex moutan extract is prepared by a double aqueous phase method, and comprises the following steps:

1) crushing cortex moutan, sieving with a No. 4 pharmacopeia sieve to obtain cortex moutan fine powder, adding the cortex moutan fine powder into an ethanol-water-sodium chloride aqueous phase serving as an extraction solvent, soaking at room temperature for 10-28 h, oscillating and extracting at the temperature of 25-50 ℃ for 2-4 h, standing for 1-2 h, and filtering to remove dregs to obtain crude extract of the cortex moutan;

2) standing the cortex moutan extraction crude liquid prepared in the step 1) at a room temperature for 1-24 h, separating the upper phase from the lower phase, removing the lower phase, and distilling the upper phase under reduced pressure to remove the solvent to obtain the cortex moutan extract.

2. The hand sanitizer according to claim 1, wherein the concentration of the aqueous sodium chloride solution in the ethanol-water-sodium chloride aqueous two-phase is 8-20%, and the volume ratio of the ethanol to the aqueous sodium chloride solution is 0.25-2: 1, the volume-mass ratio of the aqueous phase to the cortex moutan fine powder is 10 mL: 1.5 g-10 mL: 0.4 g.

3. A method of preparing a hand sanitizer according to claim 1, comprising the steps of:

1) weighing fatty alcohol-polyoxyethylene ether sodium sulfate, sodium dodecyl benzene sulfonate, sodium benzoate, EDTA-2Na and sodium chloride, pouring into a clean beaker, adding distilled water, placing into a constant-temperature water bath kettle at 25-50 ℃, and continuously stirring to completely dissolve the added components;

2) adding coconut oil fatty acid diethanolamide and glycerol into the solution obtained in the step 1), and continuously stirring to uniformly mix the coconut oil fatty acid diethanolamide and the glycerol to obtain a basic hand sanitizer;

3) weighing cortex moutan extract, adding ethanol, stirring and dissolving; slowly adding the cortex moutan extract solution into the basic hand sanitizer prepared in the step 2) under continuous stirring, and uniformly stirring to obtain the hand sanitizer.