CN104001128A - Total gingerol ointment for treating chilblain and preparation method thereof - Google Patents
Total gingerol ointment for treating chilblain and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a total gingerol ointment for treating chilblain. The total gingerol ointment is prepared from total gingerol and the following auxiliary materials in parts by weight: 2-30 parts of total gingerol, 15-70 parts of oil materials, 0-60 parts of waxes, 1-15 parts of emulsifier, 4-15 parts of humectant, 0.1-0.2 part of preservative, 0.005-0.2 part of antioxidant and 20-80 parts of water, wherein the oil materials are one or a mixture of two of grease and wax. Preparation steps for the total gingerol ointment are as follows: heating the oil materials and the emulsifier to 70-90 DEG C, and stirring to obtain an emulsified liquid; then, adding the humectant and the preservative into water, stirring continuously, and heating to 70-90 DEG C to form liquor; slowly mixing the liquor with the emulsified liquid, stirring and emulsifying, adding the antioxidant and the total gingerol while cooling to 40-50 DEG C, and uniformly stirring to prepare the ointment. The ointment disclosed by the invention is specific in active ingredient, exact in pharmacology and drug efficacy, easy to control, good in treatment effect for treating chilblain, and small in toxic and side effect.
Description
Technical field
The present invention relates to a kind of ointment for the treatment of chilblain and preparation method thereof.
Background technology
In the winter time, due to cold of weather, many people are habitually perplexed by chilblain winter.Chilblain is limitation, the infringement of erythema inflammation causing owing to being exposed to subzero cold environment, is that the upper confirmation of histology chilblain is a kind of lymphocytic vasculitis to abnormal inflammatory reaction cold, moist, non-freezing environment.Chilblain is usually expressed as single or multiple swelling scarlet or kermesinus macule, pimple or tuberosity, the visible vesicle of severe patient and festering.Conventionally with pruritus or burn feeling, chilblain easily recurs, and reduces relevant with chronic vasculitis inflammatory and the skin resistance of ill rear local skin.
The medicine for the treatment of chilblain has Western medicine, Chinese medicine at present, in the majority with Western medicine class.Western medicine often adopts nitre benzene skin pyridine etc., and toxic and side effects is larger, and therapeutic effect is not obvious, and dependency is large.The Chinese medicine for the treatment of chilblain is that the form with Chinese medicine compound exists mostly, and without index composition, pharmacological effect is imprecise, and difficult quality is controlled, and has limited its development and application as medicine.
Summary of the invention
The first object of the present invention is to provide a kind of general gingerol ointment for the treatment of chilblain, and this kind of ointment effective ingredient is clear and definite, and pharmacological effect is definite, and quality is easy to control, the good effect for the treatment of chilblain, and toxic and side effects is little.
The present invention realizes the technical scheme that its first goal of the invention adopts, and a kind of general gingerol ointment for the treatment of chilblain is mainly made by general gingerol and the adjuvant of following weight proportion:
General gingerol 2-30 part, oily raw material 15-70 part, emulsifying agent 1-15 part, wetting agent 4-15 part, antiseptic 0.1-0.2 part, antioxidant 0.005-0.2 part, water 20-80 part; Described oily raw material is the mixture of the one or both in oils and fats, wax; Described general gingerol is that gingerol compounds weight content is not less than 50% general gingerol.
In above-mentioned oily raw material, also contain polyhydric alcohol.
In above-mentioned adjuvant, also has alkaline agent 0.05-2 part.
Compared with prior art, useful achievement of the present invention is:
One, the present invention is usingd general gingerol as effective ingredient, in general gingerol, gingerol compounds weight content is higher than 50%, and gingerol constituents has the effect of obvious antiplatelet aggregation, anti-inflammatory analgesic, thereby can be not smooth to blood circulation under cold snap, blood microcirculation is obstructed and the chilblain that causes has good therapeutical effect.Its effective ingredient is clear and definite, and pharmacological effect is definite, and quality is easy to control.
Clinical trial proves that general gingerol ointment of the present invention is 100% to the treated effect of the red and swollen symptom of chilblain, treated effect to redness companion ulcer flowing water symptom is 87.5%, and existing kibe ointment (contrast) is only 71.43% to the treated effect of the red and swollen symptom of chilblain, to the treated effect of redness companion ulcer flowing water symptom, be only 50%.The two does not all find toxic and side effects, and ointment of the present invention is evident in efficacy is better than commercially available kibe ointment.Its good effect, toxic and side effects is little.
Two, in general gingerol of the present invention, gingerol compounds weight content is not less than 50%, and in the general gingerol ointment of making, effective ingredient is sufficient, and has removed the impurity of more generation special odor, thereby has guaranteed quality and the drug effect of general gingerol ointment.
Further, general gingerol of the present invention is made by following methods:
A, extraction: by Rhizoma Zingiberis dry in the shade or oven drying at low temperature after Rhizoma Zingiberis Recens section or break into powder, then in solvent, carry out merceration extraction or the reflux, extract, of 2-4 time, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of a kind of or volume fraction 50-95% in dichloromethane, ethyl acetate, acetone and ether, during each extraction, the w/v of Rhizoma Zingiberis or Rhizoma Zingiberis powder and solvent is 1kg:3-5L, the time that each merceration extracts is 24h-96h, and the time of each reflux, extract, is 1-3h;
B, purification:
Abstraction purification, adds the alcoholic solution of volume fraction 30-80% to be configured to suspension to extractum, then adds and the isopyknic petroleum ether of suspension to suspension, extracts 3-5 time, removes petroleum ether layer; Alcoholic solution layer is steamed to without alcohol taste, then add the ethyl acetate of 0.5-2 times of alcoholic solution layer volume, extract 3-5 time, recovery ethyl acetate layer, solvent evaporated, obtains general gingerol;
Or purification by chromatography, adopts purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:7-20; Type of elution is gradient elution: eluant is petrol ether/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of petroleum ether and ethyl acetate is 5-4:1, and while finishing eluting, the volume ratio of petroleum ether and ethyl acetate is 2-1:1; Or eluant is petroleum ether/acetone mixed liquor, during initial eluting, the volume ratio of petroleum ether and acetone is 7-5:1, and while finishing eluting, the volume ratio of petroleum ether and acetone is 4-2:1; Or eluant is dichloromethane/ethyl acetate mixed liquor, during initial eluting, the volume ratio of dichloromethane and ethyl acetate is 7-5:1, and while finishing eluting, the volume ratio of dichloromethane and ethyl acetate is 3-2:1; Or eluant is dichloromethane/acetone mixed liquor, during initial eluting, dichloromethane is 10-7:1 with the volume ratio of acetone, during end eluting dichloromethane with than the volume ratio of acetone, be 5-3:1.
Above general gingerol preparation method, technique is simple, easy and simple to handle, and equipment requirements is low, and production cost is low, low to operator's specification requirement, be applicable to suitability for industrialized production general gingerol, and in the general gingerol making, gingerol compounds weight content is not less than 50%.
The second object of the present invention is to provide a kind of method of preparing the general gingerol ointment of above-mentioned treatment chilblain, and the method technique is simple, production cost is low, production efficiency is high.
It is that a kind of method of preparing the general gingerol ointment of above-mentioned treatment chilblain, the steps include: that the present invention realizes the technical scheme that its second goal of the invention adopts
By the oily raw material of described proportioning and emulsifying agent be heated to 70-90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70-90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40-50 ℃, add antioxidant and the general gingerol of described proportioning, stir, can be made into ointment.
While also having alkaline agent 0.05-2 part in adjuvant, prepare the method for the general gingerol ointment of above-mentioned treatment chilblain, its step:
By the oily raw material of described proportioning and emulsifying agent be heated to 70-90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, antiseptic and alkaline agent are added in the water of described proportioning, constantly stir, be heated to 70-90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40-50 ℃, add antioxidant and the general gingerol of described proportioning, stir, can be made into ointment.
This preparation method above, can effectively make and take the ointment that general gingerol is effective ingredient, and its technique is simple, production cost is low, production efficiency is high.
Below in conjunction with the specific embodiment, the present invention is described in further detail.
The specific embodiment
Embodiment 1
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
2 parts of general gingerols, 50 parts of oily raw materials, 2 parts of emulsifying agent---glyceryl monostearates, 11 parts of wetting agent---propylene glycol, 0.5 part of alkaline agent---potassium hydroxide, 0.2 part of antiseptic---salicylic acid, 0.1 part of antioxidant---sodium sulfite, 20 parts, water.Wherein, oily raw material is oils and fats---25 parts of stearic acid, wax---10 parts of lanolines and polyhydric alcohol---mixture that octadecanol is 15 parts.
Wherein, general gingerol is made by following methods:
A, extraction: by the Rhizoma Zingiberis Recens 1kg section after drying in the shade, the merceration that then carries out 4 times in solvent extracts, and after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of volume fraction 50%, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:5L at every turn, the time 48h that each merceration extracts.
B, purification: to extractum, add the alcoholic solution of volume fraction 30% to be configured to suspension, then add and the isopyknic petroleum ether of suspension to suspension, extract 5 times, remove petroleum ether layer; Alcoholic solution layer is steamed to without alcohol taste, then add the ethyl acetate of 1 times of alcoholic solution layer volume, extract 4 times, recovery ethyl acetate layer, solvent evaporated, obtains general gingerol.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 80%.
The step of method of general gingerol ointment of preparing the treatment chilblain of this example is:
By the oily raw material of described proportioning and emulsifying agent be heated to 70 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 90 ℃ and form solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 2
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
10 parts of general gingerols, 15 parts of oily raw materials, 1 part of emulsifying agent---propylene glycol monostearate, 4 parts of wetting agent---glycerol, 1 part of alkaline agent---triethanolamine, 0.15 part of antiseptic---ethylparaben, 0.02 part of antioxidant---sodium sulfite, 40 parts, water.Wherein oily raw material is oils and fats---10 parts of white oils and wax---mixture that Cera Flava is 5 parts.
Wherein, general gingerol is made by following methods:
A, extraction: the Rhizoma Zingiberis Recens 1kg after drying at 40 ℃ is broken into powder, and the merceration that then carries out 3 times in solvent extracts, and after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of volume fraction 95%, and while extracting, the w/v of Rhizoma Zingiberis powder and solvent is 1kg:3L at every turn, the time 24h that each merceration extracts.
B, purification: to extractum, add the alcoholic solution of volume fraction 50% to be configured to suspension, then add and the isopyknic petroleum ether of suspension to suspension, extract 4 times, remove petroleum ether layer; Alcoholic solution layer is steamed to without alcohol taste, then add the ethyl acetate of 2 times of alcoholic solution layer volumes, extract 3 times, recovery ethyl acetate layer, solvent evaporated, obtains general gingerol.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 70%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 3
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
5 parts of general gingerols, 55 parts of oily raw materials, 5 parts of emulsifying agent---sorbitan monooleates (Arlacel-80), 10 parts of wetting agent---glycerol, 0.15 part of alkaline agent---potassium hydroxide, 0.12 part of antiseptic---benzalkonium bromide, 0.1 part of antioxidant---ethylenediaminetetraacetic acid, 75 parts, water.Wherein oily raw material is oils and fats---40 parts of butyl stearates and polyhydric alcohol---mixture that hexadecanol is 15 parts.
Wherein, general gingerol is made by following methods:
A, extraction: by Rhizoma Zingiberis 1kg section, the merceration that then carries out 2 times in solvent extracts, and after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of volume fraction 70%, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:4L at every turn, and the time that each merceration extracts is 96h.
B, purification: to extractum, add the alcoholic solution of volume fraction 80% to be configured to suspension, then add and the isopyknic petroleum ether of suspension to suspension, extract 3 times, remove petroleum ether layer; Alcoholic solution layer is steamed to without alcohol taste, then add the ethyl acetate of 0.5 times of alcoholic solution layer volume, extract 5 times, recovery ethyl acetate layer, solvent evaporated, obtains general gingerol.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 75%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 80 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 80 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 4
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
20 parts of general gingerols, 70 parts of oily raw materials, 6 parts of emulsifying agent---polyoxyethylene 20 sorbitan monostearates (Tween-60), 5 parts of wetting agent---propylene glycol, 1 part of alkaline agent---Borax, 0.1 part of antiseptic---propyl p-hydroxybenzoate, 0.01 part of antioxidant---succinic acid, 54 parts, water.Wherein oily raw material is oils and fats---stearic acid.
Wherein, general gingerol is made by following methods:
A, extraction: Rhizoma Zingiberis 1kg is broken into powder, then in solvent, carry out the reflux, extract, of 3 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of volume fraction 80%, and while extracting, the w/v of Rhizoma Zingiberis powder and solvent is 1kg:4L at every turn, and the time of each reflux, extract, is 2h.
B, purification: to extractum, add the alcoholic solution of volume fraction 40% to be configured to suspension, then add and the isopyknic petroleum ether of suspension to suspension, extract 5 times, remove petroleum ether layer; Alcoholic solution layer is steamed to without alcohol taste, then add the ethyl acetate of 0.5 times of alcoholic solution layer volume, extract 5 times, recovery ethyl acetate layer, solvent evaporated, obtains general gingerol.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 70%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 75 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 5
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
25 parts of general gingerols, 70 parts of oily raw materials, 13 parts of emulsifying agent---polyglycol ethers (polyoxyethylene nonylphenol ether), 12 parts of wetting agent---propylene glycol, 0.1 part of antiseptic---sorbic acid, 0.1 part of antioxidant---propyl gallate, 80 parts, water.Wherein oily raw material is wax---53 parts of hydrogenated lanolins and polyhydric alcohol---mixture that hexadecanol is 17 parts
Wherein, general gingerol is made by following methods:
A, extraction: by the Rhizoma Zingiberis Recens 1kg section after drying in the shade, the merceration that then carries out 2 times in solvent extracts, and after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is ethyl acetate, and while extracting, the w/v of Rhizoma Zingiberis powder and solvent is 1kg:5L at every turn, and the time that each merceration extracts is 72h.
B, purification: to extractum, add the alcoholic solution of volume fraction 80% to be configured to suspension, then add and the isopyknic petroleum ether of suspension to suspension, extract 3 times, remove petroleum ether layer; Alcoholic solution layer is steamed to without alcohol taste, then add the ethyl acetate of 2 times of alcoholic solution layer volumes, extract 3 times, recovery ethyl acetate layer, solvent evaporated, obtains general gingerol.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 55%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 85 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 6
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
30 parts of general gingerols, 70 parts of oily raw materials, 15 parts of emulsifying agent---cetyl adipate esters, 15 parts of wetting agent---hyaluronic acids, 2 parts of alkaline agent---triethanolamine, antiseptic---the bromo-2-of 2-nitro-1,0.1 part of ammediol, 0.02 part of antioxidant---Butylated hydroxyanisole, 50 parts, water.Wherein oily raw material is wax---vaseline.
Wherein, general gingerol is made by following methods:
A, extraction: the Rhizoma Zingiberis Recens after 40 ℃ of oven dry is broken into powder, then in solvent, carry out the reflux, extract, of 3 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is acetone, and while extracting, the w/v of Rhizoma Zingiberis powder and solvent is 1kg:4L at every turn, and the time of each reflux, extract, is 1h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:7, type of elution is gradient elution, eluant is petrol ether/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of petroleum ether and ethyl acetate is 5:1, and while finishing eluting, the volume ratio of petroleum ether and ethyl acetate is 1.5:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 50%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 7
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
15 parts of general gingerols, 15 parts of oily raw materials, 1 part of emulsifying agent---glyceryl monostearate, 5 parts of wetting agent---propylene glycol, 0.15 part of antiseptic---potassium sorbate, 0.005 part of antioxidant---dibenzylatiooluene, 25 parts, water.Wherein oily raw material is oils and fats---stearic acid.
Wherein, general gingerol is made by following methods:
A, extraction: by the Rhizoma Zingiberis Recens section after drying in the shade, then in solvent, carry out the reflux, extract, of 4 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of volume fraction 80%, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:3L at every turn, and the time of each reflux, extract, is 2h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:20, type of elution is gradient elution, eluant is petrol ether/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of petroleum ether and ethyl acetate is 4:1, and while finishing eluting, the volume ratio of petroleum ether and ethyl acetate is 1:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 65%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 70 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, antiseptic are added in the water of described proportioning, constantly stir, be heated to 80 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 45 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 8
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
15 parts of general gingerols, 70 parts of oily raw materials, 9.5 parts of emulsifying agent---aluminium stearate, 8 parts of wetting agent---propylene glycol, 0.1 part of alkaline agent---calcium hydroxide, 0.2 part of antiseptic---Potassium Benzoate, 0.1 part of antioxidant---guaiacol, 50 parts, water.Wherein oily raw material is oils and fats---45 parts of white oils and wax---mixture that ceresine is 25 parts.
Wherein, general gingerol is made by following methods:
A, extraction: the Rhizoma Zingiberis Recens after drying in the shade is broken into powder, and the merceration that then carries out 4 times in solvent extracts, and after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is ether, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:3L at every turn, and the time that each merceration extracts is 96h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:15, type of elution is gradient elution, eluant is petrol ether/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of petroleum ether and ethyl acetate is 4.5:1, and while finishing eluting, the volume ratio of petroleum ether and ethyl acetate is 2:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 55%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 80 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 9
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
3 parts of general gingerols, 40 parts of oily raw materials, 11 parts of emulsifying agent---sodium lauryl sulphates, 12 parts of wetting agent---glycerol, 0.1 part of antiseptic---ethyl hydroxybenzoate, 0.05 part of antioxidant---sodium erythorbate, 48 parts, water.Wherein, oily raw material is wax class---32 parts, vaseline and polyhydric alcohol---mixture that hexadecanol is 8 parts.
Wherein, general gingerol is made by following methods:
A, extraction: by the Rhizoma Zingiberis Recens section after 50 ℃ of oven dry, then in solvent, carry out the reflux, extract, of 4 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is ethyl acetate, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:5L at every turn, and the time of each reflux, extract, is 1h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:7, and type of elution is gradient elution, and eluant is petroleum ether/acetone mixed liquor, and during initial eluting, the volume ratio of petroleum ether and acetone is 7:1, and while finishing eluting, the volume ratio of petroleum ether and acetone is 3:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 58%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 75 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, antiseptic are added in the water of described proportioning, constantly stir, be heated to 75 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40 ℃, add antioxidant and the general gingerol of described proportioning, stir, can be made into ointment.
Embodiment 10
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
2 parts of general gingerols, 45 parts of oily raw materials, 8 parts of emulsifying agent---glyceryl monooleates, 10 parts of wetting agent---propylene glycol, 0.3 part of alkaline agent---potassium hydroxide, 0.1 part of antiseptic---citric acid, 0.1 part, antioxidant---ascorbic acid, 30 parts, water.Wherein oily raw material is oils and fats---20 parts, olive oil and wax---mixture that vaseline is 25 parts.
Wherein, general gingerol is made by following methods:
A, extraction: by Rhizoma Zingiberis 1kg section, the merceration that then carries out 2 times in solvent extracts, and after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is dichloromethane, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:4L at every turn, and the time that each merceration extracts is 60h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:10, and type of elution is gradient elution, and eluant is petroleum ether/acetone mixed liquor, and during initial eluting, the volume ratio of petroleum ether and acetone is 6:1, and while finishing eluting, the volume ratio of petroleum ether and acetone is 4:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 90%.
Preparation process:
The step of method of general gingerol ointment of preparing the treatment chilblain of this example is:
By the oily raw material of described proportioning and emulsifying agent be heated to 70 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 90 ℃ and form solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 11
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
7 parts of general gingerols, 15 parts of oily raw materials, 1 part of emulsifying agent---sodium lauryl sulphate, 5 parts of wetting agent---glycerol, 0.5 part of alkaline agent---Borax, 0.1 part of antiseptic---sodium benzoate, 0.02 part of antioxidant---sodium sulfite, 30 parts, water.Wherein oily raw material is oils and fats---5 parts, Oleum Sesami, wax---8 parts of hydrogenated lanolins and polyhydric alcohol---mixture that octadecanol is 2 parts.
Wherein, general gingerol is made by following methods:
A, extraction: Rhizoma Zingiberis 1kg is broken into powder, then in solvent, carry out the reflux, extract, of 2 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the acetone soln of volume fraction 50%, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:3L at every turn, and the time of each reflux, extract, is 3h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:20, and type of elution is gradient elution, and eluant is petroleum ether/acetone mixed liquor, and during initial eluting, the volume ratio of petroleum ether and acetone is 5:1, and while finishing eluting, the volume ratio of petroleum ether and acetone is 2:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 82%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 12
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
5 parts of general gingerols, 52 parts of oil raw materials, 3 parts of emulsifying agent---polyoxyethylene 20 sorbitan monostearates (Tween-60), 7 parts of wetting agent---glycerol, 0.1 part of alkaline agent---calcium hydroxide, 0.15 part of antiseptic---benzalkonium bromide, 0.2 part of antioxidant---ethylenediaminetetraacetic acid, 60 parts, water.Wherein oily raw material is oils and fats---stearic acid.
Wherein, general gingerol is made by following methods:
A, extraction: the Rhizoma Zingiberis Recens after drying in the shade is broken into powder, and the merceration that then carries out 2 times in solvent extracts, and after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is acetone, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:5L at every turn, and the time that each merceration extracts is 48h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:7, type of elution is gradient elution, eluant is dichloromethane/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of dichloromethane and ethyl acetate is 7:1, and while finishing eluting, the volume ratio of dichloromethane and ethyl acetate is 2:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 90%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 80 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 80 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 13
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
20 parts of general gingerols, 70 parts of oily raw materials, 10 parts of emulsifying agent---sorbitan trioleates (Arlacel-85), 5 parts of wetting agent---propylene glycol, 1.5 parts of alkaline agent---Boraxs, 0.2 part of antiseptic---propyl p-hydroxybenzoate, 0.1 part of antioxidant---propyl gallate, 65 parts, water.Wherein oily raw material is oils and fats---45 parts, Oleum Sesami, wax---22 parts, vaseline and polyhydric alcohol---mixture that hexadecanol is 3 parts.
Wherein, general gingerol is made by following methods:
A, extraction: by the Rhizoma Zingiberis Recens section after drying in the shade, then in solvent, carry out the reflux, extract, of 3 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of volume fraction 95%, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:4L at every turn, and the time of each reflux, extract, is 1h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:20, type of elution is gradient elution, eluant is dichloromethane/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of dichloromethane and ethyl acetate is 5:1, and while finishing eluting, the volume ratio of dichloromethane and ethyl acetate is 1.5:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 85%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 75 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 14
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
4 parts of general gingerols, 15 parts of oily raw materials, 15 parts of emulsifying agent---polyglycol ethers (polyoxyethylene nonylphenol ether), 15 parts of wetting agent---propylene glycol, 0.05 part of alkaline agent---calcium hydroxide, 0.1 part of antiseptic---sorbic acid, 0.1 part, antioxidant---ascorbic acid, 80 parts, water.Wherein oily raw material is wax---12 parts of lanolines and polyhydric alcohol---mixture that hexadecanol is 3 parts.
Wherein, general gingerol is made by following methods:
A, extraction: the Rhizoma Zingiberis Recens after 50 ℃ of oven dry is broken into powder, then in solvent, carry out the reflux, extract, of 4 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is dichloromethane, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:5L at every turn, and the time of each reflux, extract, is 1h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:12, type of elution is gradient elution, eluant is dichloromethane/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of dichloromethane and ethyl acetate is 6:1, and while finishing eluting, the volume ratio of dichloromethane and ethyl acetate is 3:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 75%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 85 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 15
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
30 parts of general gingerols, 48 parts of oily raw materials, 15 parts of emulsifying agent---cetyl adipate esters, 15 parts of wetting agent---hyaluronic acids, 2 parts of alkaline agent---triethanolamine, 0.2 part of antiseptic---citric acid, 0.2 part of antioxidant---Butylated hydroxyanisole, 70 parts, water.Wherein oily raw material is wax---vaseline.
Wherein, general gingerol is made by following methods:
A, extraction: by the Rhizoma Zingiberis Recens section after 45 ℃ of oven dry, then in solvent, carry out the merceration of 3 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is ether, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:5L at every turn, and the time that each merceration extracts is 24h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:7, type of elution is gradient elution, eluant is dichloromethane/acetone mixed liquor, and during initial eluting, the volume ratio of dichloromethane and acetone is 10:1, and while finishing eluting, the volume ratio of dichloromethane and acetone is 4:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 55%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 16
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
15 parts of general gingerols, 15 parts of oily raw materials, 3 parts of emulsifying agent---sodium lauryl sulphates, 3 parts of wetting agent---hyaluronic acids, 0.15 part of antiseptic---potassium sorbate, 0.005 part of antioxidant---succinic acid, 20 parts, water.Wherein oily raw material is oils and fats---9 parts of stearic acid and polyhydric alcohol---mixture that octadecanol is 6 parts.
Wherein, general gingerol is made by following methods:
A, extraction: by Rhizoma Zingiberis section, then in solvent, carry out the reflux, extract, of 4 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of volume fraction 50%, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:3L at every turn, and the time of each reflux, extract, is 3h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:20, type of elution is gradient elution, eluant is dichloromethane/acetone mixed liquor, and during initial eluting, the volume ratio of dichloromethane and acetone is 7:1, and while finishing eluting, the volume ratio of dichloromethane and acetone is 3:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 70%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, antiseptic are added in the water of described proportioning, constantly stir, be heated to 90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 17
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
10 parts of general gingerols, 70 parts of oily raw materials, 12 parts of emulsifying agent---aluminium stearate, 12 parts of wetting agent---glycerol, 0.15 part of alkaline agent---calcium hydroxide, 0.2 part of antiseptic---Potassium Benzoate, 0.1 part of antioxidant---guaiacol, 55 parts, water.Wherein oily raw material is oils and fats---58 parts of isopropyl myristates and polyhydric alcohol---mixture that hexadecanol is 12 parts.
Wherein, general gingerol is made by following methods:
A, extraction: the Rhizoma Zingiberis Recens after drying in the shade is broken into powder, then in solvent, carry out the reflux, extract, of 2 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is acetone, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:5L at every turn, and the time of each reflux, extract, is 2h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:15, type of elution is gradient elution, eluant is dichloromethane/acetone mixed liquor, and during initial eluting, the volume ratio of dichloromethane and acetone is 9:1, and while finishing eluting, the volume ratio of dichloromethane and acetone is 5:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 65%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 80 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Embodiment 18
Treat a general gingerol ointment for chilblain, by general gingerol and the adjuvant of following weight proportion, made:
2 parts of general gingerols, 15 parts of oil raw materials, 3 parts of emulsifying agent---polyoxyethylene 20 sorbitan monostearates (Tween-60), 7 parts of wetting agent---glycerol, 0.05 part of alkaline agent---calcium hydroxide, 0.15 part of antiseptic---benzalkonium bromide, 0.2 part of antioxidant---ethylenediaminetetraacetic acid, 60 parts, water.Wherein oily raw material is wax---lanoline isopropyl alcohol.
Wherein, general gingerol is made by following methods:
A, extraction: the Rhizoma Zingiberis Recens after drying in the shade is broken into powder, then in solvent, carry out the reflux, extract, of 2 times, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is ether, and while extracting, the w/v of Rhizoma Zingiberis and solvent is 1kg:5L at every turn, and the time of each reflux, extract, is 1h.
B, purification: adopt purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:12, type of elution is gradient elution, eluant is dichloromethane/ethyl acetate mixed liquor, and during initial eluting, the volume ratio of dichloromethane and ethyl acetate is 5:1, and while finishing eluting, the volume ratio of dichloromethane and ethyl acetate is 2:1.
Test shows, in the general gingerol that this example makes, gingerol compounds weight content is 87%.
Preparation process:
By the oily raw material of described proportioning and emulsifying agent be heated to 75 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 80 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 45 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
Clinical observation on the therapeutic effect
One, data and method
1, case selection
Collect typical chilblain disease patient 50 examples, red and swollen symptom's 42 examples wherein, red and swollen and with affected part ulcer flowing water person 8 examples, patient age scope is 18～60 years old, without gestation or women breast-feeding their children, allergic constitution or to drug allergy person.Set up matched group 50 examples, the aspects such as treatment group and matched group Gender, age and the state of an illness are basic identical simultaneously.Two groups of contrasts are without significant difference, and P>0.05, has comparability.
2, Therapeutic Method
Matched group is used kibe ointment (Guangdong Taicheng Pharmaceutical Co., Ltd's productions), one day twice, early, evening respectively once.
Treatment group is used the present invention's general gingerol ointment, one day twice, early, evening each once.
Two groups were all used by one week identical course for the treatment of, and during treating, stop using other medicines.
3, observational technique
During medication, observe once every day, comprises symptom improvement situation, untoward reaction.
Two, efficacy determination
1, efficacy assessment standard
Cure: ulcer wound surface healing, smooth, red and swollen dissipation, sufferings disappear completely;
Effective: ulcer wound surface healing, redness, purple red color spot dissipates to some extent, and sufferings disappear;
Effective: ulcer wound surface heals substantially, erubescence swell and itch disease alleviates to some extent;
Invalid: ulcer wound surface does not change, erubescence swell and itch disease is not improved.
2, statistical method
Enumeration data adopts X
2check, measurement data adopts t check.
Total effective rate=cure rate+obvious effective rate+effective percentage
Three, result
1, red and swollen improvement situation is in Table 1, and red and swollen ulcer flowing water disease is improved situation in Table 2.
The red and swollen improvement situation of table 1 (84 example)
Treatment group curative effect is obviously better than matched group (p<0.05)
The red and swollen companion of table 2 ulcer flowing water symptom is improved situation (16 example)
Treatment group curative effect is obviously better than matched group (p<0.05)
2, untoward reaction: treatment group patient and matched group patient all have no adverse reaction.
Oils and fats that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful oils and fatss, as oleic acid, Petiolus Trachycarpi oil, Oleum Camelliae, isopropyl palmitate, isopropyl myristate, Ethylhexyl stearate, dimethicone, methyl phenyl silicone oil, polyethylene glycol stearate diester.
Wax that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful wax, as acetylated lanolin, acetylated lanolin, spermaceti, insect wax, microwax.
Polyhydric alcohol that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful polyhydric alcohol, as lauryl alcohol.
The emulsifying agent that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful emulsifying agents, as: potassium stearate (magnesium, calcium, zinc, manganese), triethanolamine oleate, polyoxyethylene sorbitol Cera Flava derivant, Wool wax alcohols,ethoxylated derivant, oleic alcohol polyoxyethylene ether, fatty alcohol-polyoxyethylene ether, polyoxyethylene lauryl ether, alkylphenol-polyethenoxy alcohol, aliphatic alcohol polyoxyvinethene phosphate sodium salt, polyoxyethylene 20 sorbitan monopalmitate (Tween-40), polyoxyethylene 20 sorbitan tristearate (Tween-65), polyoxyethylene 20 sorbitan trioleate (tween 85), sorbitan mono-laurate (Arlacel-20), anhydrous sorbitol monopalmitate (Arlacel-40), sucrose fatty acid ester, ten two (16, 18) zephiran.
Wetting agent that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful wetting agents, as 1,3 butylene glycol, Polyethylene Glycol, sorbic acid.
Alkaline agent that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful alkaline agents, as sodium hydroxide, ammonium hydroxide.
The antiseptic that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful antiseptic, as: methyl parahydroxybenzoate (Nepal's methyl ester, methyl hydroxybenzoate), butyl p-hydroxybenzoate (Nepal's butyl ester, butyl hydroxybenzoate), benzoic acid, dehydroacetic acid, boric acid, sodium propionate, calcium propionate, benzyl alcohol, imidazolidinyl, benzalkonium chloride, KF88.
The antioxidant that the present invention adopts except embodiment listed, also comprise various existing pharmaceutically useful antioxidant, as: dihydric phenol, 2,5-di-tertiary butyl methyl phenol, 2,5-di-tert-butyl hydroquinone, guaiac resin, vitamin E, sodium erythorbate, glutamic acid, disodiumedetate.
Claims (6)
1. treat a general gingerol ointment for chilblain, mainly by general gingerol and the adjuvant of following weight proportion, made: general gingerol 2-30 part, oily raw material 15-70 part, emulsifying agent 1-15 part, wetting agent 4-15 part, antiseptic 0.1-0.2 part, antioxidant 0.005-0.2 part, water 20-80 part; Described oily raw material is the mixture of the one or both in oils and fats, wax; Described general gingerol is that gingerol compounds weight content is not less than 50% general gingerol.
2. a kind of general gingerol ointment for the treatment of chilblain according to claim 1, is characterized in that, in described oily raw material, also contains polyhydric alcohol.
3. a kind of general gingerol ointment for the treatment of chilblain according to claim 1 and 2, is characterized in that, also has alkaline agent 0.05-2 part in described adjuvant.
4. a kind of general gingerol ointment for the treatment of chilblain according to claim 1, is characterized in that described general gingerol is made by following methods:
A, extraction: by Rhizoma Zingiberis dry in the shade or oven drying at low temperature after Rhizoma Zingiberis Recens section or break into powder, then in solvent, carry out merceration extraction or the reflux, extract, of 2-4 time, after having extracted, merge extractive liquid,, evaporate to dryness solvent are to extractum;
Described solvent is the alcoholic solution of a kind of or volume fraction 50-95% in dichloromethane, ethyl acetate, acetone and ether, during each extraction, the w/v of Rhizoma Zingiberis or Rhizoma Zingiberis powder and solvent is 1kg:3-5L, the time that each merceration extracts is 24h-96h, and the time of each reflux, extract, is 1-3h;
B, purification:
Abstraction purification, adds the alcoholic solution of volume fraction 30-80% to be configured to suspension to extractum, then adds and the isopyknic petroleum ether of suspension to suspension, extracts 3-5 time, removes petroleum ether layer; Alcoholic solution layer is steamed to without alcohol taste, then add the ethyl acetate of 0.5-2 times of alcoholic solution layer volume, extract 3-5 time, recovery ethyl acetate layer, solvent evaporated, obtains general gingerol;
Or purification by chromatography, adopts purification on normal-phase silica gel column chromatography to carry out purification, merge stream part that chromatography obtains, evaporate to dryness eluant, obtains general gingerol; During purification by chromatography, the mass ratio of extractum and chromatographic silica gel is 1:7-20, type of elution is gradient elution: eluant is petrol ether/ethyl acetate mixed liquor, during initial eluting, the volume ratio of petroleum ether and ethyl acetate is 5-4:1, and while finishing eluting, the volume ratio of petroleum ether and ethyl acetate is 2-1:1; Or eluant is petroleum ether/acetone mixed liquor, during initial eluting, the volume ratio of petroleum ether and acetone is 7-5:1, and while finishing eluting, the volume ratio of petroleum ether and acetone is 4-2:1; Or eluant is dichloromethane/ethyl acetate mixed liquor, during initial eluting, the volume ratio of dichloromethane and ethyl acetate is 7-5:1, and while finishing eluting, the volume ratio of dichloromethane and ethyl acetate is 3-2:1; Or eluant is dichloromethane/acetone mixed liquor, during initial eluting, dichloromethane is 10-7:1 with the volume ratio of acetone, during end eluting dichloromethane with than the volume ratio of acetone, be 5-3:1.
5. a method of preparing the general gingerol ointment of the treatment chilblain described in claim 1 or 2, the steps include:
By the oily raw material of described proportioning and emulsifying agent be heated to 70-90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70-90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40-50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
6. a method of preparing the general gingerol ointment for the treatment of chilblain claimed in claim 3, the steps include:
By the oily raw material of described proportioning and emulsifying agent be heated to 70-90 ℃ be stirred to emulsifying even emulsion; Then the wetting agent of described proportioning, alkaline agent, antiseptic are added in the water of described proportioning, constantly stir, be heated to 70-90 ℃ to forming solution; By this solution and emulsion slowly mix, stirring and emulsifying, while being cooled to 40-50 ℃, add antioxidant and the general gingerol of described proportioning, stir, obtain.
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