A kind of nematicidal composition containing carbosulfan and its application
Technical field
The present invention relates to a kind of nematicidal composition, more particularly to a kind of nematicidal composition containing carbosulfan and
It prevents and treats the application of crops nematode, belongs to technical field of pesticide.
Background technology
Plant nematode mainly includes:Meloidogyne (Meloidogyne), kidney shape turbatrix
(Rotylenchulus), cyst roundworm (Heterodera (Heterodera)), root spot nematode (Pratylenchus
(Pratykenchus)).Eelworm harm influences the absorption of Plant To Nutrient and moisture, returns phytopathogen invasion and provides just
Profit.At present, there is problems with the chemical agent of preventing and treating root-knot nematode:(1) residues of pesticides are high, and (2) input cost is high, and (3) prevent
Effect is not notable enough, and (4) have much room for improvement to the yield and quality of crop.
Carbosulfan is carbofuran low toxicity derivative, and insecticidal effect is close to carbofuran, but toxicity only has carbofuran
1/10th.It is a kind of wide spectrum, systemic insecticide, it is adaptable to paddy rice, corn and soybean, potato, sugarcane, cotton, fruit tree
Deng crop, various pests are prevented and treated.
Tioxazafen, chemical name:3- phenyl -5- (thiophene -2- bases) -1,2,4- oxadiazoles are Meng Shan all 2008 years
The non-stifling property nematode agent of exploitation, because its is more environmentally friendly, using safer, the influence to people is smaller, is described as the ideal of lythidathion
Substitute.Indoor and field experiment shows that control of the performance equivalent to or better than current commercialization of the new nematicide is produced
Product.It is reported that tioxazafen is to the soy bean cyst roundworm of soybean, root-knot nematode, kidney shape nematode, the pratylenchus on corn,
It is living that the phytopathy original nematodes such as kidney shape nematode, root-knot nematode on root-knot nematode, long line worm, cotton are respectively provided with fabulous biology
Property.
Up to now, the open source literature of carbosulfan and tioxazafen compoundings is not yet inquired.
The content of the invention
It is an object of the invention to provide a kind of nematicidal composition containing carbosulfan, and help to delay line
Worm produces resistance, improves prevention effect, reduction effective ingredient consumption and use cost etc..
Inventor after a large amount of Seiving Tests, is having been surprisingly found that carbosulfan and tioxazafen by certain ratio
There is notable synergistic effect to Plant nematode after example mixing.
The present composition has synergistic function, and is therefore particularly suitable for preventing and treating plant nematode, especially
Parasitic nematode in cereal crop, oil crops, sugar crop, cotton crudefiber crop, gardening gardens class plant.
The nematicidal composition that the present invention is provided, it is characterised in that:Containing active ingredient carbosulfan and
tioxazafen.Both mass ratioes are 1:50-50:1, preferred mass ratio is 1:30-30:1, more preferably mass ratio is 1:10-
10:1。
The present composition can also prevent and treat various crops such as corn, paddy rice, wheat, barley, oat, rye, soybean, flower
Harmful organism on the plant such as life, ornamental plant, sugarcane, cotton and in seed.
The present composition is particularly suitable for preventing and treating plant nematode, such as Pratylenchidae (Pratykenchus
Spp), Radopholus similis Throne (Radopholous similis), fuller's teasel Ditylenchus dipsaci (Ditylenchus dipsaci), partly wear
Pierce nematode (Tylenchulus semipenetrans), Heterodera kind (Heterodera spp.), ball Heterodera
Plant (Globodera spp.), Meloidogyne kind (Meloidogyne spp.), kidney shape turbatrix kind (Rotylenchulus
Spp.), Aphelenchoides kind (Aphelenchoides spp.), minute hand Turbatrix kind (Longidorus spp.), sword nematode
Category kind (Xiphinema spp.), burr Turbatrix kind (Trichodorus spp.), Bursaphelenchus kind
(Bursaphelenchus spp.)。
It is preferred that by the present composition be used for prevent and treat the oil crops such as soybean and peanut, Cereal, paddy rice, wheat, cotton,
The nematodiasis of flowers etc., such as citrus root-knot nematode, soy bean cyst roundworm, peanut root-knot nematode.
The present composition can be prepared into active ingredient in the formulation of suitable agricultural use, preparation with known method
Total content is 5%-80%, and remaining is pesticide adjuvant composition.
The nematicidal composition of the present invention can be formulated as being available for of spraying or progress soil or seed treatment after dilution
Granula, water dispersible granules, suspending agent, microemulsion, aqueous emulsion, microcapsule suspending agent or suspension seed-coating agent.
Preparation is prepared in a known way, for example, prepared by the way that active component is mixed with solvent and/or carrier, if desired
If use other pesticide adjuvant compositions such as emulsifying agent and dispersant.
Insecticide-applying way can take spraying, pouring root, soak seed, get rid of and apply, spread fertilizer over the fields, ditch spread, spreading manuer in holes, preferred ditch during preventing and treating nematode
Apply, spread manuer in holes, the soil treatment such as pouring root or leaching root, seed soaking, seed pelleting processing.
Compared with prior art, what nematicidal composition of the present invention was produced has the beneficial effect that:(1) compared with single dose, synergy
Substantially, prevention effect is improved;(2) field dosage can be greatly decreased, reduction causes poisoning, the possibility poisoned, reduction
Production and use cost, and it is effectively reduced environmental pollution and residues of pesticides;(3) mechanism of action of active ingredient is mutual in composition
Differ, reduce the single selection pressure to nematode, be conducive to overcoming nematode resistance to produce and delaying the drug-fast production of nematode
Raw speed;(4) nematicidal composition has good preventive effect to all kinds of nematodes.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, present invention specific examples below
Illustrate, but the present invention is only limited to absolutely not these examples.
The synergistic function of the present composition can pass through following experiment explanation:
Assays Example:Carbosulfan compounds the Toxicity Determination to peanut root-knot nematode with tioxazafen
Experiment
Subjects:Meloidogyne incognita (Meloidogyne incognita)
Evaluation method is pot-culture method.The pieces of an egg aseptic water washing of picking root-knot nematode, is placed in culture dish from Roots of Peanut
Wet filter paper on, add 20ml sterilized waters, hatch at 20 DEG C -25 DEG C, obtain age consistent 2 instar larvaes, diluted with sterilized water
To 200/ml.2 instar larvaes are inoculated with the leaf phase of peanut two, using root injection method, take 5ml nematodes inoculation liquid to inject rhizosphere soil
It is interior, 24h soil conservations 70%-80% humidity after inoculation.It is standby after peanut inoculation root-knot nematode.
Active component is made into mother liquor after dissolving on request, suitable concentration is then diluted to purified water carries out root irrigation.
Fresh branches and leaves of peanut seedling is placed in phjytotron after chemicals treatment, then 25-30 DEG C, 12h alternation of light and darkness, relative humidity 35%-
30d is cultivated under the conditions of 50%.Each to repeat the basin of random searching 4, each processing amounts to investigation 16 potted flowers life.Grade scale is in
Magnificent people's republic's national standard《Pesticide field efficacy medicine test criterion (one)》.
Diseased plant stage division is as follows:
0 grade:Root system is without insect gall;
1 grade:Root system has a small amount of small insect gall;
3 grades:2/3rds root systems are covered with small insect gall;
5 grades:Root system is covered with small insect gall and has secondary insect gall;
7 grades:Root system formation fibrous root group.
Computational methods:
The expection effect (i.e. theoretical prevention effect, %) of mixture of active principles determined using Colby formula and with observation
To effect compare:
Colby formula:E=X+Y-X*Y/100
E:When active components A concentration is a in composition, compositions contemplated when active component B concentrations are b is imitated
Power,
X:Effect when active components A dosage is a,
Y:Effect when active component B dosages are b.
When the prevention effect observed exceedes expected preventive effect, explanation has synergistic effect.
Carbosulfan and tioxazafen compoundings are to the Toxicity Determination result of the test of peanut root-knot nematode referring to table
1、2。
Toxicity Determination result of the test of the single active component of table 1. to peanut root-knot nematode
The carbosulfan of table 2. and tioxazafen compound the Toxicity Determination result of the test to peanut root-knot nematode
Result of the test shows that carbosulfan and tioxazafen compounding preventing and treating peanut root-knot nematodes are matched 1:50-
50:When between 1, all with synergistic effect.
Nematicidal composition of the present invention can be prepared into granule, the water-dispersible grain of suitable agricultural use with known method
Agent, suspending agent, microemulsion, aqueous emulsion, microcapsule suspending agent and suspension seed-coating agent.Illustrated, be formulated with specific embodiment below
Middle percentage is mass percent.Active component refers to carbosulfan and tioxazafen, active component in present specification
100% purity meter is pressed, in actual application, is converted according to active compound actual purity used, difference filler or dilution
Agent is adjusted, and is below repeated no more.
First, suspending agent processing and application example
The each components such as active component, wetting dispersing agent, thickener, carrier and water are well mixed in the ratio of formula, passed through
Suspending agent is obtained after grinding and/or high speed shear.
Embodiment 1:51% carbosulfan tioxazafen suspending agents carbosulfan 50%, tioxazafen
1%, methyl naphthalene sulfonate formaldehyde condensate 10%, sodium lignin sulfonate 10%, xanthans 1%, organosilicon 1%, glycerine
5%, water complements to 100%.
The embodiment is applied to prevent and treat groundnut root knot nematode disease.51% carbosulfan tioxazafen suspending agents are pressed
Effective ingredient consumption 200g a.i./hm2The prevention effect for being diluted with water 30 days and 60 days after pouring ground, medicine is respectively 84.2% He
81.3%.The carbosulfan suspending agent of comparison medicament 5% and 10%tioxazafen suspending agents press effective ingredient consumption 225g
a.i./hm2, in kind use.The preventive effect of 30 days and 60 days is respectively 67.6% He after 5% carbosulfan suspending agent medicine
61.8%;The preventive effect of 30 days and 60 days is respectively 70.5% and 68.8% after 10%tioxazafen suspending agent medicines.Fourth sulphur gram hundred
With synergistic effect after tioxazafen compoundings substantially, the preventive effect to peanut root-knot nematode is significantly better than single dose to prestige.
Embodiment 2:51% carbosulfan tioxazafen suspending agents
Carbosulfan 1%, tioxazafen 50%, thermally coupled distillation columns 8%, carboxymethyl cellulose
1%, white carbon 1%, glycerine 4%, water complements to 100%.
The embodiment is applied to preventing and treating corn root-knot nematode.By 51% carbosulfan tioxazafen suspending agents by having
Imitate Ingredient Amount 200g a.i./hm2The prevention effect for being diluted with water 30 days and 60 days after carry out root irrigation, medicine is respectively
84.1% and 81.5%.The carbosulfan suspending agent of comparison medicament 5% and 10%tioxazafen suspending agents press active ingredient
Consumption 225g a.i./hm2, in kind use, the preventive effect difference of 30 days and 60 days after 5% carbosulfan suspending agent medicine
For 71.4% and 67.8%;The preventive effect of 30 days and 60 days is respectively 73.4% He after 10%tioxazafen suspending agent medicines
74.5%.With synergistic effect after tioxazafen compoundings substantially, the preventive effect to corn root-knot nematode is significantly better than carbosulfan
Single dose.
2nd, aqueous emulsion processing and application example
Active component, solvent, emulsifying agent are added together, make to be dissolved into homogeneous oil phase;Under high velocity agitation, by oil phase plus
Enter in water and be well mixed, aqueous emulsion is made.
Embodiment 3:10% carbosulfan tioxazafen aqueous emulsions
Carbosulfan 5%, tioxazafen 5%, 1-METHYLPYRROLIDONE 10%, calcium dodecyl benzene sulfonate 5%,
Agriculture breast 600#5%, water complements to 100%.
The embodiment is applied to preventing and treating citrus root-knot nematode.By 10% carbosulfan tioxazafen aqueous emulsions by having
Imitate Ingredient Amount 200g a.i./hm2The prevention effect for being diluted with water 30 days and 60 days after pouring ground, medicine is respectively 89.4% He
86.2%.The carbosulfan missible oil of comparison medicament 5% and 20%tioxazafen missible oil press effective ingredient consumption 225g
a.i./hm2, in kind use.The preventive effect of 30 days and 60 days is respectively 73.1% He after 5% carbosulfan missible oil medicine
70.4%;The preventive effect of 30 days and 60 days is respectively 75.8% and 76.3% after 20%tioxazafen missible oil medicines.Carbosulfan
With synergistic effect after tioxazafen compoundings substantially, the preventive effect to citrus root-knot nematode is significantly better than single dose.
3rd, microemulsion processing and application example
Active component, solvent, cosolvent, emulsifying agent are added together, make to be dissolved into homogeneous oil phase;By water-soluble substances plus
Enter in water, be dissolved into homogeneous aqueous phase, it is under agitation, oil phase is mixed with water uniform, microemulsion is made.
Embodiment 4:31% carbosulfan tioxazafen microemulsion carbosulfan 1%, tioxazafen30%,
1-METHYLPYRROLIDONE 10%, isopropanol 10%, agriculture breast 500#5%, agriculture breast 1601#5%, water complements to 100%.
The embodiment is applied to preventing and treating root-knot nematode.By 31% carbosulfan tioxazafen microemulsions by having
The prevention effect that effect 8g/100 kilograms of seed of composition is diluted with water 30 days and 60 days after seed soaking, medicine is respectively 72.9% He
71.3%.The carbosulfan missible oil of comparison medicament 5% and 20%tioxazafen missible oil press 10g/100 kilograms of kind of active ingredient
Son, is in kind used, and is investigated within 30 days and 60 days after medicine, 5% carbosulfan missible oil preventive effect is respectively 62.4% He
53.7%;20%tioxazafen missible oil preventive effects are respectively 66.1% and 60.3%.Carbosulfan is compounded with tioxazafen
Substantially, the preventive effect to root-knot nematode is significantly better than single dose for synergistic effect afterwards.
Embodiment 5:31% carbosulfan tioxazafen microemulsions carbosulfan 30%, tioxazafen
1%, 1-METHYLPYRROLIDONE 10%, isopropanol 10%, agriculture breast 500#5%, agriculture breast 1601#5%, water complements to 100%.
The embodiment is applied to preventing and treating great Jiang root-knot nematodes.By 31% carbosulfan tioxazafen microemulsions by having
Imitate Ingredient Amount 200g a.i./hm2The prevention effect for being diluted with water 30 days and 60 days after pouring root, medicine is respectively 84.2% He
81.7%.The carbosulfan missible oil of comparison medicament 5% and 20%tioxazafen missible oil press effective ingredient consumption 225g
a.i./hm2, in kind use, the preventive effect of 30 days and 60 days is respectively 74.1% He after 5% carbosulfan missible oil medicine
72.5%;The preventive effect of 30 days and 60 days is respectively 76.8% and 72.6% after 20%tioxazafen missible oil medicines.Carbosulfan
With synergistic effect after tioxazafen compoundings substantially, the preventive effect to great Jiang root-knot nematodes is significantly better than single dose.
4th, suspension seed-coating agent processing and application example
Embodiment 6:11% carbosulfan tioxazafen suspension seed-coating agents
Carbosulfan 10%, tioxazafen 1%, APES (dispersant) 3%, pull open powder (wetting
Agent) 1%, xanthans (thickener) 0.2%, the aqueous emulsion of dimethyl polysiloxane fluid (defoamer) of ethylene glycol (antifreezing agent) 4%, 20%
0.4%th, acrylic resin (film forming agent) 2%, sodium benzoate (preservative) 0.25%, colouring agent, water are supplemented to 100%, according to
Prepared by conventional method can obtain 11% carbosulfan tioxazafen suspension seed-coating agents.
The embodiment is applied to cotton root-knot nematode.11% carbosulfan tioxazafen suspension seed-coating agents are pressed
300g/100kg cotton seeds are dressed seed, and the prevention effect of 30 days and 60 days is respectively 84.3% and 80.2% after medicine.Comparison medicament
5% carbosulfan suspension seed-coating agent and 20%tioxazafen suspension seed-coating agents are dressed seed by 300g/100kg cotton seeds.
The preventive effect of 30 days and 60 days is respectively 71.7% and 73.5% after 5% carbosulfan suspension seed-coating agent medicine;20%
The preventive effect of 30 days and 60 days is respectively 74.8% and 72.1% after tioxazafen suspension seed-coating agent medicines.Carbosulfan with
Substantially, the preventive effect to cotton root-knot nematode is significantly better than single dose for synergistic effect after tioxazafen compoundings.
5th, microcapsule suspending agent processing and application example
Embodiment 7:33% carbosulfan tioxazafen microcapsule suspending agents
Carbosulfan 3%, tioxazafen 30%, macromolecule cyst material polymer MDI3%, solvent linseed oil
5% and S-200 solvent naphthas 9%, emulsifying agent triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer 3.5%, dispersant
Negel 5%.Above-mentioned active ingredient, macromolecule cyst material, solvent are mixed, make to be dissolved into homogeneous oil phase, is emulsified
Agent, dispersant is soluble in water is prepared into homogeneous aqueous phase, in a shear condition, oil phase is added in aqueous phase solution, emulsion is prepared into,
Stirring is lower to add 10% ethylenediamine solution 4%, is warming up to 65 DEG C of insulation reaction 4.0h, and two kinds of materials occur anti-in oil-water interfaces
Should, form high score ascus wall, you can 33% carbosulfan tioxazafen microcapsule suspending agents are made.
The embodiment is applied to sugarcane root-knot nematode.By 33% carbosulfan tioxazafen microcapsule suspending agents by having
Imitate Ingredient Amount 200g a.i./hm2Be diluted with water pouring ground, after medicine the prevention effect of 30 days and 60 days be respectively 83.5%,
84.8%.The carbosulfan microcapsule suspending agent of comparison medicament 5% presses effective ingredient consumption 300g a.i./hm2, 10%
Tioxazafen microcapsule suspending agents press effective ingredient consumption 225g a.i./hm2, in kind use.5% carbosulfan
The preventive effect of 30 days and 60 days is respectively 72.1% and 73.7% after microcapsule suspending agent medicine;10%tioxazafen microcapsule suspending agent medicines
The preventive effect of 30 days and 60 days is respectively 76.4% and 77.2% afterwards.Synergistic effect is bright after carbosulfan is compounded with tioxazafen
Aobvious, the preventive effect to sugarcane root-knot nematode is significantly better than single dose.
6th, water dispersible granules processing and application example
Active component, dispersant, wetting agent, disintegrant and filler are well mixed in the ratio of formula, through air-flow crushing
Into wettable powder, add a certain amount of water mixing extruding and make material.Water dispersible granules are obtained after drying screening.
Embodiment 8:80% carbosulfan tioxazafen water dispersible granules
Carbosulfan 15%, tioxazafen 65%, Negel 4%, sodium lignin sulfonate 2%, dodecane
Base sodium sulphate 5%, amine sulfate 2%, kaolin complements to 100%.
The embodiment is applied to preventing and treating wheat cyst roundworm.By 80% carbosulfan tioxazafen water dispersible granules
By effective ingredient consumption 200g a.i./hm2The prevention effect for being diluted with water 30 days and 60 days after pouring ground, medicine is respectively 84.2%
With 81.3%.The carbosulfan suspending agent of comparison medicament 5% and 10%tioxazafen suspending agents press effective ingredient consumption
225g a.i./hm2, in kind use.The preventive effect of 30 days and 60 days is respectively after 5% carbosulfan suspending agent medicine
73.1% and 66.4%;The preventive effect of 30 days and 60 days is respectively 75.7% and 74.3% after 10%tioxazafen suspending agent medicines.
With synergistic effect after tioxazafen compoundings substantially, the preventive effect to wheat cyst roundworm is significantly better than single dose to carbosulfan.
7th, granule processing and application example
Active component, other auxiliary elements are well mixed successively in the ratio of formula, is coated and granule is made
Embodiment 9:10% carbosulfan tioxazafen granules
Carbosulfan 5%, tioxazafen 5%, white carbon 6%, adhesive 1%, colouring agent 0.5%, granular carbonic acid
Calcium complements to 100%.
The embodiment is applied to preventing and treating barley root-knot nematode.By 10% carbosulfan tioxazafen granules by having
Imitate Ingredient Amount 200g a.i./hm2Ditch spread is handled, and the prevention effect of 30 days and 60 days is respectively 80.3% and 75.2% after medicine.
The carbosulfan granule of comparison medicament 0.5% presses effective ingredient consumption 300g a.i./hm2, 5%tioxazafen granules
By effective ingredient consumption 225g a.i./hm2, in kind use, 30 days and 60 after 0.5% carbosulfan Granular pesticide
It preventive effect is respectively 71.4% and 63.2%;The preventive effect of 30 days and 60 days is respectively after 5%tioxazafen Granular pesticides
74.3% and 72.1%.Carbosulfan and synergistic effect after tioxazafen compoundings are obvious, to the preventive effect of barley root-knot nematode
Significantly better than single dose.
Embodiment 10:5% carbosulfan tioxazafen granules
Carbosulfan 2%, tioxazafen 3%, adhesive 1%, colouring agent 0.5%, dispersant 0.5%, silica sand is mended
Enough to 100%.
The embodiment is applied to preventing and treating peanut root-knot nematode.By 5% carbosulfan tioxazafen granules by having
Imitate Ingredient Amount 200g a.i./hm2Ditch spread is handled, and the prevention effect of 30 days and 60 days is respectively 84.2% and 83.2% after medicine.
The carbosulfan granule of comparison medicament 0.5% presses effective ingredient consumption 300g a.i./hm2, 5%tioxazafen granules
By effective ingredient consumption 225g a.i./hm2, in kind use.30 days and 60 after 0.5% carbosulfan Granular pesticide
It preventive effect is respectively 75.1% and 67.8%;The preventive effect of 30 days and 60 days is respectively after 5%tioxazafen Granular pesticides
74.3% and 75.7%.Carbosulfan and synergistic effect after tioxazafen compoundings are obvious, to the preventive effect of peanut root-knot nematode
Significantly better than single dose.