CN107296850B - Traditional Chinese medicine gel product for treating canine pyocutaneous disease and preparation method thereof - Google Patents

Traditional Chinese medicine gel product for treating canine pyocutaneous disease and preparation method thereof Download PDF

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CN107296850B
CN107296850B CN201710725241.6A CN201710725241A CN107296850B CN 107296850 B CN107296850 B CN 107296850B CN 201710725241 A CN201710725241 A CN 201710725241A CN 107296850 B CN107296850 B CN 107296850B
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filtrate
gel
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CN107296850A (en
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刘娟
罗艺晨
刘丽
杨显朝
杨庆昌
程邓芳
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Southwest University
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Abstract

The invention relates to a product of a traditional Chinese medicine gel for treating canine skin and external diseases and a preparation method thereof, and the product is characterized in that lithospermum, rhodiola rosea and angelica dahurica are extracted by an ethanol warm immersion method, then angelica, radix sileris, radix rehmanniae and liquorice are mixed with medicine residues and extracted by a water extraction method, the mixture is respectively concentrated into clear paste, and then the clear paste is prepared into the gel together with a humectant, a gel matrix and the like.

Description

Traditional Chinese medicine gel product for treating canine pyocutaneous disease and preparation method thereof
Technical Field
The invention belongs to the field of traditional Chinese medicine preparations, relates to a preparation method of a traditional Chinese medicine gel for treating canine pyocutaneous disease, and also relates to a product prepared by the method.
Background
Veterinary drugs are drugs used for the prevention and treatment of various animal diseases under the guidance of the Chinese veterinary theory. The Chinese medicine such as the lithospermum, the angelica and the like are commonly prepared into the burn oil in China, the burn oil has obvious curative effect on infectious wounds and ulcer surfaces such as burns, scalds, wounds and the like, and can also be prepared into dosage forms such as ointment and the like for clinical application. The ointment is rich in viscosity, when the ointment is externally applied to an affected part, a thin film is easily formed to be tightly attached to a wound surface when the ointment is infiltrated for a lot, bleeding is easy to occur when the ointment is removed, the wound surface can be lubricated by the oil solution without feeling of hardness, adhesion and discomfort, the adhesiveness is small, the medicine stays on the affected part for a short time, and the ointment and the oil solution are easy to pollute clothes.
Sores and ulcers are a large group of diseases in surgery of traditional Chinese medicine, which are divided into acute diseases and chronic diseases, the causes of the diseases are divided into two types, namely exogenous pathogenic factors and endogenous wound, sores and ulcers caused by exogenous pathogenic factors mainly attack the exterior of the body by pathogenic factors such as heat toxin, fire toxin mixed with wind cold, summer heat and dampness, block qi and blood, are trapped in muscles and are left in muscles and bones to cause sores and ulcers, and the theory of Su Wen and Sheng Qi Tong Tian says that: failure of nutrient qi to transform into nutrient qi means that it will cause carbuncle and swelling, which can cause furuncle, carbuncle, furuncle, skin ulcer, cellulitis and mass. Sores and ulcers caused by internal injury are mainly caused by weakness of five zang organs and disharmony of six fu organs or blockage of meridians, and are classified into yin syndrome and yang syndrome. For example, the five internal organs are mostly pathogenic due to deficiency, and are mostly chronic, for example, the five internal organs are empty due to kidney deficiency, and sores and ulcers which are easily caused by wind-cold phlegm invasion to cause phlegm, lung and kidney yin deficiency, deficient fire flaming up, scrofula caused by fluid burning as phlegm and have the primary and middle stages of sores and ulcers and unsmooth channels and collaterals are mostly yin syndromes, and the sores and ulcers are characterized by slow onset, sore-shaped collapse and diffuse, no pain or dull pain, pain pumping, unchanged skin color, purple dark or deep black, no heat or slight heat; difficult to eliminate, difficult to open and difficult to heal, and when the pus is opened, the disease course is long and the recovery is slow; the excessive heart fire ' pain, itch and sore ' belong to the heart ', the sores and ulcers caused by the excessive heart fire, insufficient heart yin, blood dryness, wind generation, improper diet, internal injury to spleen and stomach and transformation of heart and stomach into stagnated fire belong to yang syndrome, are mainly characterized by red, swelling, heat and pain and are often accompanied by exogenous symptoms such as cold and cold limbs, and the like, and have the disadvantages of acute onset, quick recovery, easy ulceration, thick, moist pus and color after ulceration and easy elimination. In addition, sores and ulcers caused by endogenous qi are usually more severe than those caused by exogenous pathogenic factors, such as diabetes complicated with furuncle and gangrene, although healthy qi may not be weakened yet. Therefore, it is generally accepted that the occurrence of sores and ulcers is mild in the affected part and severe in the affected part.
Gels are a generic term for a class of macromolecular network systems containing two or more components, including liquid semisolid jellies and dried systems (gels) thereof. The gel preparation is prepared by dissolving or uniformly dispersing the medicine in the gel, has the advantages of good adhesiveness and permeability, comfortable use, easy cleaning and the like, and can make up for the defects of the oil preparation. In modern pharmacy, the gel is used as a sustained-release and controlled-release preparation formulation of a matrix, the medicines suitable for a gel administration system comprise hydrophilic medicines, hydrophobic medicines, acidic medicines, macromolecular medicines and the like, and can be administered from various ways such as oral cavity, nasal cavity, skin and the like, but the traditional Chinese medicine gel has the problem of slow release and absorption speed of active ingredients, so that the traditional Chinese medicine gel for treating the pyocutaneous disease is prepared by necessarily selecting proper auxiliary materials and controlling the proportion.
Disclosure of Invention
In view of the above, the present invention provides a preparation method of a traditional Chinese medicine gel for treating canine pyocutaneous disease, and the present invention provides a traditional Chinese medicine gel for treating canine pyocutaneous disease prepared by the above method.
In order to achieve the purpose, the invention provides the following technical scheme:
1. a preparation method of a traditional Chinese medicine gel for treating canine pyocutaneous disease comprises the following steps:
1) extracting medicinal materials: placing 30-60 parts by weight of lithospermum erythrorhizon, 30-50 parts by weight of rhodiola rosea and 10-16 parts by weight of angelica dahurica in an ethanol solution with the volume fraction of 50% for leaching and filtering, and respectively collecting filtrate and filter residue which are marked as filtrate I and filter residue I; then adding 15-30 parts by weight of angelica sinensis, 10-18 parts by weight of radix sileris, 10-18 parts by weight of radix rehmanniae and 4-12 parts by weight of liquorice into the filter residue I, then adding water for extraction, filtering, collecting filtrate, marking as filtrate II, and respectively concentrating the filtrate I and the filtrate II to obtain clear paste with the relative density of 1.32-1.50 for later use;
2) preparation of gel matrix: taking 2-5 parts of borneol by weight, and adding glycerol which is 2 times of the weight of the borneol for dissolving to prepare borneol solution; then taking 2.5-6 parts of carbomer by weight, putting the carbomer in distilled water for full swelling, adjusting the pH to 6-7 by using a sodium hydroxide solution to prepare a carbomer solution, adding carboxymethyl cellulose into hot water for full dissolution, adding glycerol and 10-20 parts of propylene glycol to prepare a carboxymethyl cellulose solution, wherein the addition amount of the glycerol is 15 parts by weight of the total weight of the added glycerol, and uniformly mixing the prepared carbomer solution and the carboxymethyl cellulose solution to prepare a gel matrix;
3) preparing a traditional Chinese medicine gel: adding the prepared borneol solution and the concentrated clear paste of the filtrate II into a gel matrix, then adding the concentrated clear paste of the filtrate I, adding 10-20 parts by weight of pearl powder, mixing and stirring uniformly, and preparing the traditional Chinese medicine gel.
The main raw materials of the traditional Chinese medicine composition have the following properties and efficacies:
radix Arnebiae is dry root of Arnebia euchroma (Royle) Johnst; sweet, salty and cold in nature; heart and liver meridian entered; has the functions of cooling blood, promoting blood circulation, removing toxicity and promoting eruption, and is mainly used for treating exuberant blood heat toxin, sores and ulcers, eczema, scald caused by hot water and fire, warm spot rash, burn, eczema and erysipelas.
The radix Rhodiolae is dry root of radix Rhodiolae (Rhodiola rosea L.) belonging to Rhodiola of Crassulaceae; sweet, astringent and cold in nature; entering lung meridian; the functions of the medicine are mainly activating blood circulation to stop bleeding, dissipating blood stasis and relieving swelling, and the medicine is mainly used for treating heat and sore, venomous scab and tumescence, arthralgia due to cold and heat wind and traumatic injury.
Radix Angelicae sinensis is dried root of Angelica sinensis (Oliv.) Diels) of Umbelliferae; sweet and pungent in flavor and warm in nature; it enters liver, heart and spleen meridians; has the functions of enriching blood and promoting blood circulation, and is mainly used for treating skin numbness, carbuncle, deep-rooted carbuncle, pyocutaneous disease and traumatic injury.
Radix Saposhnikoviae is dried root of radix Saposhnikoviae (Saposhnikovia divaricata (Trucz.) Schischk.) belonging to family Umbelliferae, genus Saposhnikovia; pungent and sweet in flavor and slightly warm in nature; it enters bladder, lung, spleen and liver meridians; has the functions of dispelling wind, relieving exterior syndrome, eliminating dampness, relieving pain and relieving spasm.
Radix rehmanniae is root tuber of Rehmannia glutinosa Libosch.ex Fisch.et Mey (Rehmannia glutinosa (Gaetn.) of Rehmannia of Scrophulariaceae); sweet, bitter and slightly cold in taste; enter heart, liver and kidney meridians; has effects in nourishing yin, clearing away heat, cooling blood, and tonifying blood; can be used for treating macula, hematemesis, and epistaxis.
Radix Angelicae Dahuricae is dried root of Angelica dahurica (Angelica Dahurica) of Angelica of Umbelliferae; warm in nature, pungent in flavor, fragrant in smell and slightly bitter; it enters lung and stomach meridians. Has the functions of promoting blood circulation, expelling pus, promoting granulation and relieving pain.
Borneolum is prepared from fresh branches and leaves of Cinnamomum camphora (L) presl of Lauraceae by steam distillation and recrystallization; fragrant smell, pungent and bitter taste, cool and volatile; enter heart and liver meridians; it can dispel stagnated fire, induce bone heat, remove blood-shot eyes, nebula, acne and poison, promote granulation and alleviate pain.
The main components of the pearl powder comprise calcium carbonate, taurine, trace elements required by human body, and amino acids (glycine, methionine, alanine, leucine, glutamic acid, etc.) required by human body, and has effects of removing toxic substance and promoting granulation; sores in the mouth and tongue; ulceration of the throat; the sore is not healed for a long time, and has the effects of cooling and detoxifying, calming the nerves and arresting convulsion, removing nebula and promoting tissue regeneration, whitening, removing wrinkles and ageing, eliminating color spots, and maintaining beauty.
According to the prevention and treatment principle and the principle of a traditional Chinese medicine formula, nine medicines of lithospermum, rhodiola rosea, angelica, radix sileris and the like with the functions of detoxifying and promoting eruption, cooling blood and activating blood circulation and dissipating blood stasis and reducing swelling are selected to form a reasonable formula: zi Cao is a monarch drug for cooling blood and activating blood circulation, removing toxicity and resolving macula. The rhodiola root has the efficacies of tonifying qi and clearing away the lung-heat, benefiting intelligence and nourishing heart, astringing and stopping bleeding, and dissipating blood stasis and eliminating swelling, and is used as a ministerial drug. Chinese angelica, pungent and sweet in flavor and warm in property, is fragrant and dispersing in nature, can tonify blood and activate blood, and is the essential herb for tonifying blood and regulating nutrient. Fang Feng is pungent, sweet and mild in flavor, can dispel wind and relieve exterior syndrome, and can reach the whole body, and is the main herb of wind, so qi circulation is the blood circulation. Radix rehmanniae has the functions of clearing heat and cooling blood, nourishing yin and promoting the production of body fluid, enters blood system and has special effect on bleeding caused by recklessly blood flow due to blood heat. Radix Angelicae Dahuricae has the effects of dispelling pathogenic wind, relieving exterior syndrome, relieving swelling, expelling pus, growing muscle, removing putrefaction and promoting tissue regeneration, and is called as "Chuang Jia Sheng Yao". Borneol has the effects of inducing resuscitation, refreshing mind, clearing heat, relieving pain, preventing corrosion and relieving itching. The pearl powder has the functions of detoxifying and promoting tissue regeneration, has a special effect on skin damage caused by knife wound, bruise, scald, burn and the like, and is an adjuvant drug. Licorice root, radix Glycyrrhizae has the effects of tonifying middle-jiao and Qi, relieving spasm and pain, and harmonizing the drugs, and is used as a guiding drug. The medicines are used together, and the effects of clearing and activating the channels and collaterals, clearing away heat and toxic materials, promoting blood circulation and relieving pain, healing sore and promoting granulation are achieved.
The auxiliary material glycerin can be used as a humectant and a solvent, can increase the transparency of the gel, and is also used as a humectant and a preservative of the gel, but because the water content of the gel is higher, a certain amount of propylene glycol is added as the preservative; meanwhile, the propylene glycol can be used as a humectant and a solvent, and can also be used as a preservative and a gel matrix; however, the dosage of propylene glycol in the formulation may affect the appearance of the gel, so that it is necessary to control the appropriate amount.
The humectant can be replaced by sorbitol, polyethylene glycol and hyaluronic acid, and the preservative can be replaced by benzalkonium bromide, methylparaben and phenoxyethanol.
The gel matrix can be replaced by hypromellose, methylcellulose, chitosan and gelatin. The carbomer is used, and the carbomer is a high-molecular polymer formed by crosslinking acrylic acid and propenyl deer sugar, so that the carbomer has the characteristics of strong hygroscopicity, no irritation to skin, transparent film formed after coating, good heat resistance and cold resistance, strong adhesiveness, easiness in cleaning, no greasy feeling, capability of absorbing tissue exudate, no irritation to skin and the like; meanwhile, the sodium carboxymethyl cellulose is used as an external gel matrix by utilizing various functions of adhesion, emulsification, slow release and the like, but the sodium carboxymethyl cellulose is easy to lose water to a certain extent, so that the formed gel preparation is dry and lacks water, and the problem is solved by using the sodium carboxymethyl cellulose and humectants such as glycerin and the like.
In the invention, the extraction of the medicinal materials in the step 1) is as follows: 50 parts of lithospermum, 50 parts of rhodiola rosea and 15 parts of angelica dahurica are taken according to the weight ratio and are placed into an ethanol solution with the volume fraction of 50 percent for leaching and filtering, and filtrate and filter residue are respectively collected and are marked as filtrate I and filter residue I; then adding 25 parts by weight of angelica, 15 parts by weight of radix sileris, 15 parts by weight of radix rehmanniae and 10 parts by weight of liquorice into the filter residue I, then adding water for extraction, filtering, collecting filtrate, marking as filtrate II, and respectively concentrating the filtrate I and the filtrate II into clear paste with the relative density of 1.32-1.50 for later use;
preferably, in the step 1), the adding amount of the ethanol solution is 8 times of the weight of the lithospermum, the rhodiola rosea and the angelica dahurica.
Preferably, in step 1), the leaching is performed at 60 ℃ for 12 hours.
Preferably, in the step 1), the water extraction is performed by adding an aqueous solution which is 10 times of the weight of the angelica, the radix sileris, the radix rehmanniae, the liquorice and the filter residue I, and extracting for 3 times at 100 ℃ for 3 hours each time.
2. The traditional Chinese medicine gel for treating canine pyocutaneous disease prepared by the preparation method.
The invention has the beneficial effects that: the invention discloses a preparation method of a traditional Chinese medicine gel for treating canine pyocutaneous disease, which is characterized in that a mauve semisolid traditional Chinese medicine preparation is obtained by optimizing a main formula and auxiliary materials and combining a preparation process, the texture is uniform and fine, a water-soluble mechanism is provided, the greasiness and the stuffiness of liposome are avoided, the preparation has good biological adhesion, the preparation can be precisely adhered to an action part within a long time after being coated, the medicine can be slowly and uniformly released, the bioavailability is high, the adverse reaction of the medicine is reduced, and the medicine using frequency is reduced. The dosage and quality of each component of the prepared gel meet the general standard of 'Chinese veterinary drug, natural drug classification and registration data requirement' in No. 442 bulletin of Ministry of agriculture, 'the compilation of technical guidance of veterinary drug research, and' pharmacopoeia of the people's republic of China' 2015 edition (two parts). The properties, pH value, viscosity and qualitative identification of the gel accord with the regulations, all detection items have no obvious change, the defects of poor water absorption, easy rancidity, difficult storage and the like of a grease matrix in the ointment are overcome through a reasonable formula, a good foundation is laid for the quality control of the gel, technical optimization and technical theoretical support are provided for ensuring the safety and the effectiveness of gel products, and the traditional Chinese patent medicine is enabled to really realize safety, effectiveness, stability and controllability.
Detailed Description
The preferred embodiments of the present invention will be described in detail below.
Example 1 preparation method of Ziyu granulation promoting gel
The medicinal materials are respectively weighed according to the following prescription, and the specific formula is as follows:
Figure GDA0002687736940000041
Figure GDA0002687736940000051
the preparation method comprises the following steps:
1) extracting medicinal materials: soaking the lithospermum, the rhodiola rosea and the angelica dahurica in 50 (v/v)% ethanol solution which is 8 times of the weight of the medicinal materials at 60 ℃ for 12 hours, filtering, and respectively collecting filtrate I and filter residue; then adding the angelica, the radix sileris, the radix rehmanniae and the liquorice with the prescription amount into the collected filter residue, adding aqueous solution which is 10 times of the weight of the medicinal materials, extracting for 3 times at 100 ℃ for 3 hours each time, filtering, collecting filtrate II, and respectively concentrating the filtrate I and the filtrate II to obtain clear paste (the relative density is 1.32-1.50) for later use;
2) preparation of gel matrix: dissolving the borneol in the prescription amount in glycerol which is 2 times of the weight of the borneol to prepare borneol solution; taking carbomer, placing into distilled water, swelling, adjusting pH to 6-7 with sodium hydroxide solution, adding carboxymethyl cellulose into hot water, dissolving, adding the rest glycerol and propylene glycol, and mixing the dissolved carbomer and carboxymethyl cellulose solution to obtain gel matrix;
3) the preparation of the purple jade granulation promoting gel comprises the following steps: adding the obtained Borneolum solution and the concentrated fluid extract of filtrate II into gel matrix, mixing, stirring, adding the concentrated fluid extract of filtrate I, adding Margarita powder, mixing, stirring, making into ZIYUSHENGJI gel, and packaging.
Example 2 preparation method of Ziyu granulation promoting gel
The medicinal materials are respectively weighed according to the following prescription, and the specific formula is as follows:
Figure GDA0002687736940000052
Figure GDA0002687736940000061
the preparation method comprises the following steps:
1) extracting medicinal materials: soaking the lithospermum, the rhodiola rosea and the angelica dahurica in 50 (v/v)% ethanol solution which is 8 times of the weight of the medicinal materials at 60 ℃ for 12 hours, filtering, and respectively collecting filtrate I and filter residue; then adding the angelica, the radix sileris, the radix rehmanniae and the liquorice with the prescription amount into the collected filter residue, adding aqueous solution which is 10 times of the weight of the medicinal materials, extracting for 3 times at 100 ℃ for 3 hours each time, filtering, collecting filtrate II, and respectively concentrating the filtrate I and the filtrate II to obtain clear paste (the relative density is 1.32-1.50) for later use;
2) preparation of gel matrix: dissolving the borneol in the prescription amount in glycerol which is 2 times of the weight of the borneol to prepare borneol solution; taking carbomer, placing into distilled water, swelling, adjusting pH to 6-7 with sodium hydroxide solution, adding carboxymethyl cellulose into hot water, dissolving, adding the rest glycerol and propylene glycol, and mixing carbomer and carboxymethyl cellulose solution to obtain gel matrix;
3) the preparation of the purple jade granulation promoting gel comprises the following steps: adding the obtained Borneolum solution and the concentrated fluid extract of filtrate II into gel matrix, mixing, stirring, adding the concentrated fluid extract of filtrate I, adding Margarita powder, mixing, stirring, making into ZIYUSHENGJI gel, and packaging.
Example 3 preparation method of Ziyu granulation promoting gel
The medicinal materials are respectively weighed according to the following prescription, and the specific formula is as follows:
Figure GDA0002687736940000062
the preparation method comprises the following steps:
1) extracting medicinal materials: soaking the lithospermum, the rhodiola rosea and the angelica dahurica in 50 (v/v)% ethanol solution which is 8 times of the weight of the medicinal materials at 60 ℃ for 12 hours, filtering, and respectively collecting filtrate I and filter residue; then adding the angelica, the radix sileris, the radix rehmanniae and the liquorice with the prescription amount into the collected filter residue, adding aqueous solution which is 10 times of the weight of the medicinal materials, extracting for 3 times at 100 ℃ for 3 hours each time, filtering, collecting filtrate II, and respectively concentrating the filtrate I and the filtrate II to obtain clear paste (the relative density is 1.32-1.50) for later use;
2) preparation of gel matrix: dissolving the borneol in the prescription amount in glycerol which is 2 times of the weight of the borneol to prepare borneol solution; taking carbomer, placing into distilled water, swelling, adjusting pH to 6-7 with sodium hydroxide solution, adding carboxymethyl cellulose into hot water, dissolving, adding the rest glycerol and propylene glycol, and mixing carbomer and carboxymethyl cellulose solution to obtain gel matrix;
3) the preparation of the purple jade granulation promoting gel comprises the following steps: adding the obtained Borneolum solution and the concentrated fluid extract of filtrate II into gel matrix, mixing, stirring, adding the concentrated fluid extract of filtrate I, adding Margarita powder, mixing, stirring, making into ZIYUSHENGJI gel, and packaging.
Example 4 Effect of Ziyu granulation promoting gel on rat pyocutaneous disease model
Test animals: SD rats of cleaning grade 250-300g and 12 males in 9-10 weeks are purchased from the center of experimental animals in Rongchang school zone of university in southwest university and are purchased for one week after being kept separately.
Test drugs: beeswax and pearl powder are purchased from Tong Jun Ge pharmacy. The analytical pure types of glycerin, carbomer, carboxymethyl cellulose and propylene glycol were used.
Test strains: staphylococcus aureus CCVC2261, detection of chinese veterinary drugs.
Preparation of bacterial suspension: selecting a colony from the plate, inoculating into 2ml broth, culturing at 37 deg.C for 6 hr, plating, counting, and diluting to 10%9cfu/ml。
Preparation of rat skin: in the early stage of the test, 12 male rats were harvested and approximately 20cm of hair was cut on both sides of the dorsal spine of the rats2The part is shaved and the skin is exposed, wiped clean and ready for use.
Establishing a rat pyocutaneous disease model: after the rats are fasted for 12 hours, the rats are anesthetized by intraperitoneal injection by using a proper amount of quick-sleeping new injection, local disinfection is carried out on a depilated area, an ellipse with the diameter of the long axis of 1.5cm and the diameter of the short axis of 1cm is formed on the two sides of the spinal column by about 2cm, the whole skin is cut off to reach the fascia layer, and 4 layers of sterilization gauze and 2 layers of medical adhesive tapes are covered and sewn and fixed. 0.5ml of 10 was inoculated per wound91 time per day of cfu/ml staphylococcus aureus for 3 consecutive days, uncovering the wound on day 4, cleaning the wound with physiological saline, starting the application of the drug, and exposing the wound.
The treatment method comprises the following steps: the test is divided into 5 days and 10 days, the administration time is from when the wound surface is uncovered, 3 rats in each group are randomly divided into a model group (the group is not administered and is only cleaned by normal saline); examples 1-3 Ziyu granulation promoting gel, each group is administered 1 time per day, the wound surface is cleaned with normal saline before administration, and a proper amount of gel is applied to the periphery of the wound surface for 5 or 10 consecutive days.
Measuring the wound healing area: after 5 days or 10 days of administration, the major axis and the minor axis of the wound of each group of rats were measured, and the area of the wound was calculated according to the area of the ellipse.
And (3) data analysis: the data were processed using statistical software SPSS 20.0, mean. + -. standard deviation (x. + -.s), and the results are shown in Table 1.
TABLE 1 influence of external use Ziyu granulation promoting gel on wound area of rat
Figure GDA0002687736940000081
Figure GDA0002687736940000082
Note: the same letters in the same column indicate no significant difference. The difference in letters on the same column indicates significant difference, where small letters are used to indicate a level of 0.05 and capital letters indicate a level of 0.01.
The results show that the wound area of the external-use Ziyu Shengji gel is obviously different from that of the model group on the 5 th day, and the wound areas of the Ziyu Shengji gels of the embodiments I to III have no obvious difference. The wound surface has obvious shrinkage on the 10 th day, the wound surface area coated with the purple jade tissue regeneration gel of the embodiments I-III is respectively shrunk to 0.20 +/-0.03, 0.21 +/-0.02 and 0.21 +/-0.07, the purple jade tissue regeneration gel of the embodiments I-III has obvious difference with the model group, and the comparison of the purple jade tissue regeneration gel of the embodiments I-III shows that the purple jade tissue regeneration gel of the embodiment I has obvious difference with other two groups, which shows that the purple jade tissue regeneration gel of the embodiment I can obviously reduce the wound surface area and promote the healing effect of the wound surface. The prepared purple jade granulation promoting gel can promote the healing effect of sores and has obvious convergence effect on the pyogenic infections of rats. The purple jade granulation promoting gel of the embodiment I has the best effect.
Example 5 Effect of Ziyu granulation promoting gel on dog pyocutaneous disease
Test animals: 10-week-old dogs, 12 dogs and males are purchased from the center of experimental animals in Rong Chang school district of university in south west and purchased and kept separately for one week for later use.
Test drugs: beeswax and pearl powder are purchased from Tong Jun Ge pharmacy. The analytical pure types of glycerin, carbomer, carboxymethyl cellulose and propylene glycol were used.
Test strains: staphylococcus aureus CCVC2261, detection of chinese veterinary drugs.
Preparation of bacterial suspension: one colony from the plate was inoculated into 2mL broth, incubated for 6h at 37 ℃ incubator, plated, counted and diluted to 109cfu/mL。
Preparing dog skin: in the early stage of the test, 12 puppies are taken, and the hair is sheared on the two sides of the dorsal spine of the puppies by about 20cm2The part is shaved and the skin is exposed, wiped clean and ready for use.
Building a dog sore model: after the dog is fasted for 12 hours, the dog is subjected to intraperitoneal injection anesthesia by using a proper amount of quick-sleeping new injection, local disinfection is carried out on a depilated area, an ellipse with the diameter of the long axis of 1.5cm and the diameter of the short axis of 1cm is formed on the two sides of the spinal column by about 2cm, the whole skin is cut off and reaches the fascia layer deeply, and 4 layers of sterilization gauze and 2 layers of medical adhesive tapes are covered for sewing and fixing. 0.5mL of 10 per wound was inoculated91 time per day of cfu/mL Staphylococcus aureus, continuously inoculating for 3 days, uncovering the wound surface on the 4 th day, cleaning the wound surface with physiological saline, and applying the medicine to expose the wound surface.
The treatment method comprises the following steps: the test is divided into 5 days and 10 days, the administration time is from when the initial surface is uncovered, 3 dogs in each group are randomly divided into a model group (the group is not administered and is only washed by normal saline); examples 1-3 Ziyu granulation promoting gel, each group is administered 1 time per day, the wound surface is cleaned with normal saline before administration, and a proper amount of gel is applied to the periphery of the wound surface for 5 or 10 consecutive days. Measuring the wound healing area: after the medicine is taken for 5 days or 10 days, the major axis and the minor axis of the wound of each group of dogs are measured, and the area of the wound is calculated according to the area of the ellipse.
And (3) data analysis: statistical software SPSS 20.0 for the test was used to process the data, mean. + -. standard deviation
Figure GDA0002687736940000091
The results are shown in Table 2.
TABLE 2 influence of external use Ziyu granulation promoting gel on dog wound area
Figure GDA0002687736940000092
Figure GDA0002687736940000093
Note: the same letters in the same column indicate no significant difference. The difference in letters on the same column indicates significant difference, where small letters are used to indicate a level of 0.05 and capital letters indicate a level of 0.01.
The results show that the wound area of the external-use Ziyu Shengji gel is obviously different from that of the model group on the 5 th day, the wound area is obviously reduced, and the Ziyu Shengji gels of the examples I-III have no obvious difference on the wound area. The wound area is obviously reduced on the 10 th day, the wound surfaces of the purple jade tissue regeneration gel of the embodiments I-III are respectively contracted to 0.21 +/-0.04, 0.23 +/-0.02 and 0.25 +/-0.05, the purple jade tissue regeneration gel of the embodiments I-III is extremely different from a model group, and the treatment effects of the purple jade tissue regeneration gel of the embodiments I-III are compared, so that the purple jade tissue regeneration gel of the embodiment I is obvious in difference compared with other two groups, and the purple jade tissue regeneration gel of the embodiment I can obviously reduce the wound area and promote the wound healing effect. Test results show that the purple jade granulation promoting gel prepared by the invention can promote the healing effect of sores and has an obvious convergence effect on canine pyogenic infections, and the purple jade granulation promoting gel prepared in the embodiment I has the best effect.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.

Claims (6)

1. A preparation method of a traditional Chinese medicine gel for treating canine pyocutaneous disease is characterized by comprising the following steps:
1) extracting medicinal materials: placing 30-60 parts by weight of lithospermum erythrorhizon, 30-50 parts by weight of rhodiola rosea and 10-16 parts by weight of angelica dahurica in an ethanol solution with the volume fraction of 50% for leaching and filtering, and respectively collecting filtrate and filter residue which are marked as filtrate I and filter residue I; then adding 15-30 parts by weight of angelica sinensis, 10-18 parts by weight of radix sileris, 10-18 parts by weight of radix rehmanniae and 4-12 parts by weight of liquorice into the filter residue I, then adding water for extraction, filtering, collecting filtrate, marking as filtrate II, and respectively concentrating the filtrate I and the filtrate II to obtain clear paste with the relative density of 1.32-1.50 for later use;
2) preparation of gel matrix: taking 2-5 parts of borneol by weight, and adding glycerol which is 2 times of the weight of the borneol for dissolving to prepare borneol solution; then taking 2.5-6 parts of carbomer by weight, putting the carbomer in distilled water for full swelling, adjusting the pH to 6-7 by using a sodium hydroxide solution to prepare a carbomer solution, adding carboxymethyl cellulose into hot water for full dissolution, adding glycerol and 10-20 parts of propylene glycol to prepare a carboxymethyl cellulose solution, wherein the addition amount of the glycerol is 15 parts by weight of the total weight of the added glycerol, and uniformly mixing the prepared carbomer solution and the carboxymethyl cellulose solution to prepare a gel matrix;
3) preparing a traditional Chinese medicine gel: adding the prepared borneol solution and the concentrated clear paste of the filtrate II into a gel matrix, then adding the concentrated clear paste of the filtrate I, adding 10-20 parts by weight of pearl powder, mixing and stirring uniformly, and preparing the traditional Chinese medicine gel.
2. The preparation method of the traditional Chinese medicine gel for treating canine pyocutaneous disease according to claim 1, which is characterized in that: the extraction of the medicinal materials in the step 1) comprises the following steps: 50 parts of lithospermum, 50 parts of rhodiola rosea and 15 parts of angelica dahurica are taken according to the weight ratio and are placed into an ethanol solution with the volume fraction of 50 percent for leaching and filtering, and filtrate and filter residue are respectively collected and are marked as filtrate I and filter residue I; then adding 25 parts by weight of angelica, 15 parts by weight of radix sileris, 15 parts by weight of radix rehmanniae and 10 parts by weight of liquorice into the filter residue I, then adding water for extraction, filtering, collecting filtrate II, and concentrating the filtrate I and the filtrate II respectively to obtain clear paste with the relative density of 1.32-1.50 for later use.
3. The preparation method of the traditional Chinese medicine gel for treating canine pyocutaneous disease according to claim 1 or 2, which is characterized in that: in the step 1), the adding amount of the ethanol solution is 8 times of the weight of the lithospermum, the rhodiola rosea and the angelica dahurica.
4. The preparation method of the traditional Chinese medicine gel for treating canine pyocutaneous disease according to claim 1 or 2, which is characterized in that: in step 1), the leaching is carried out at 60 ℃ for 12 hours.
5. The preparation method of the traditional Chinese medicine gel for treating canine pyocutaneous disease according to claim 1, which is characterized in that: in the step 1), the water adding extraction is to add water solution which is 10 times of the weight of the angelica, the radix sileris, the radix rehmanniae, the liquorice and the filter residue I to extract for 3 times at 100 ℃ and 3 hours each time.
6. A Chinese medicinal gel for treating canine pyocutaneous disease, prepared by the preparation method of any one of claims 1 to 5.
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