CN107296155A - 一种减少羊抗血清制备过程中羊免疫应激反应的饲料 - Google Patents
一种减少羊抗血清制备过程中羊免疫应激反应的饲料 Download PDFInfo
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Abstract
本发明公开了减少羊抗血清制备过程中羊免疫应激反应的饲料，按重量份包括：玉米50~60份、银杏叶提取物10~20份、陈皮1~3份、柠檬酸0.1~0.5份、松针粉10~30份、番薯藤10~20份、骨粉5~10份、豆渣5~10份、维生素A0.001~0.01份、维生素E0.001~0.01份、维生素C0.001~0.01份、硫酸钴0.01~0.03份。本发明的优点在于：有效改善免疫后羊的嗳气功能，提高羊的食欲，增加免疫后羊所需的各种营养物质，减少羊的免疫的应激反应，并有利于抗体的产生。
Description
技术领域
本发明属于生物技术免疫动物的饲养领域，具体涉及一种减少羊抗血清制备过程中羊免疫应激反应的饲料。
背景技术
在羊抗人血清制备的免疫过程中，通常通过安排合理的饲喂制度，做好饲养卫生和消毒工作，精粮与辅粮的科学配比来提高羊的健康水平，从而提高抗血清的产量、效价及质量。但是动物在免疫后，引起食欲下降、嗳气次数减少无力或停止，严重影响羊的消化功能，造成后续采血量减少和血清混合效价降低。
发明内容
为了克服现有技术的不足，本发明的目的在于提供一种减少羊抗血清制备过程中羊免疫应激反应的饲料。
为达到上述目的，本发明是通过以下的技术方案来实现的。
一种减少羊抗血清制备过程中羊免疫应激反应的饲料，按重量份包括：玉米50～60份、银杏叶提取物10～20、陈皮1～3份、柠檬酸0.1～0.5份、松针粉10～30份、番薯藤10～20份、骨粉5～10份、豆渣5～10份、维生素A0.001～0.01份、维生素E0.001～0.01份、维生素C0.001～0.01份、硫酸钴0.01～0.03份。
进一步地所述银杏叶提取物为银杏叶经乳酸菌发酵3～7天后，烘干粉碎制得。
进一步地所述乳酸菌为植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌组成的混合菌种。
进一步地所述混合菌种中植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌的体积比为4-5：3-4：2-3。
进一步地提供了一种减少羊抗血清制备过程中羊免疫应激反应的饲料的制备方法，包括以下步骤：
(1)将植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌按体积比4-5：3-4：2-3的比例混合制得混合菌种；
(2)将银杏叶洗净、粉碎，加入2倍重量的水，加入银杏叶重量1～3％的混合菌种，温度25～35℃，密封发酵3～7天，取出烘干，粉碎，得银杏叶提取物；
(3)将玉米50～60份、银杏叶提取物10～20份、陈皮1～3份、柠檬酸0.1～0.5份、松针粉10～30份、番薯藤10～20份、骨粉5～10份、豆渣5～10份、维生素A0.001～0.01份、维生素E0.001～0.01份、维生素C0.001～0.01份、硫酸钴0.01～0.03份混合均匀即得。
进一步地所述步骤(1)中分别取植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种一环在平板上划线，在培养箱中30℃培养24小时；取MRS固体培养基平板所长出的菌落一环接入装有300ml种子培养液的三角瓶中，置于摇床，培养条件：转速80rpm，温度30℃，恒温培养24小时，得各菌种发酵菌种液。
有益效果：与现有技术相比，本发明的优点在于：有效改善免疫后羊的嗳气功能，提高羊的食欲，增加免疫后羊所需的各种营养物质，减少羊的免疫的应激反应，并有利于抗体的产生。
具体实施方式
下面结合实例对本发明作进一步的详细说明。本发明所用的菌种及其他原料均为市售产品。
实施例1
一种减少羊抗血清制备过程中羊免疫应激反应的饲料的制备方法，包括以下步骤：
(1)分别取植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种一环在平板上划线，在培养箱中30℃培养24小时；取MRS固体培养基平板所长出的菌落一环接入装有300ml种子培养液的三角瓶中，置于摇床，培养条件：转速80rpm，温度30℃，恒温培养24小时，得各菌种发酵菌种液；将植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种液按体积比4：4：2的比例混合制得混合菌种；
(2)将银杏叶洗净、粉碎，加入2倍重量的水，加入银杏叶重量1％的混合菌种，温度25℃，密封发酵7天，取出烘干，粉碎，得银杏叶提取物；
(3)将玉米50份、银杏叶提取物20份、陈皮1份、柠檬酸0.5份、松针粉10份、番薯藤20份、骨粉5份、豆渣10份、维生素A0.01份、维生素E0.001份、维生素C0.01份、硫酸钴0.01份混合均匀即得。
日均每只羊喂料500克，午后喂些青草或青贮饲料，每只0.25千克。
实施例2
一种减少羊抗血清制备过程中羊免疫应激反应的饲料的制备方法，包括以下步骤：
(1)分别取植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种一环在平板上划线，在培养箱中30℃培养24小时；取MRS固体培养基平板所长出的菌落一环接入装有300ml种子培养液的三角瓶中，置于摇床，培养条件：转速80rpm，温度30℃，恒温培养24小时，得各菌种发酵菌种液；将植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种液按体积比5：3：3的比例混合制得混合菌种；
(2)将银杏叶洗净、粉碎，加入2倍重量的水，加入银杏叶重量2％的混合菌种，温度30℃，密封发酵5天，取出烘干，粉碎，得银杏叶提取物；
(3)将玉米55份、银杏叶提取物15份、陈皮2份、柠檬酸0.3份、松针粉20份、番薯藤15份、骨粉8份、豆渣8份、维生素A0.01份、维生素E0.001份、维生素C0.001份、硫酸钴0.03份混合均匀即得。
日均每只羊喂料500克，午后喂些青草或青贮饲料，每只0.25千克。
实施例3
一种减少羊抗血清制备过程中羊免疫应激反应的饲料的制备方法，包括以下步骤：
(1)分别取植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种一环在平板上划线，在培养箱中30℃培养24小时；取MRS固体培养基平板所长出的菌落一环接入装有300ml种子培养液的三角瓶中，置于摇床，培养条件：转速80rpm，温度30℃，恒温培养24小时，得各菌种发酵菌种液；将植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种液按体积比4：3：3的比例混合制得混合菌种；
(2)将银杏叶洗净、粉碎，加入2倍重量的水，加入银杏叶重量3％的混合菌种，温度35℃，密封发酵3天，取出烘干，粉碎，得银杏叶提取物；
(3)将玉米60份、银杏叶提取物10份、陈皮3份、柠檬酸0.1份、松针粉30份、番薯藤10份、骨粉10份、豆渣5份、维生素A0.005份、维生素E0.005份、维生素C0.005份、硫酸钴0.02份混合均匀即得。
日均每只羊喂料500克，午后喂些青草或青贮饲料，每只0.25千克。
在其他条件基本一致的前提下，我们对新旧饲料配方免疫产品进行了实际数据验证，见下表：
以上数据得出，采用本发明的饲料喂养，能明显改善免疫后羊的嗳气功能，提高羊的食欲，增加免疫后羊所需的各种营养物质，减少羊的免疫的应激反应，并有利于抗体的产生。
本发明按照上述实施例进行了说明，应当理解，上述实施例不以任何形式限定本发明，凡采用等同替换或等效变换方式所获得的技术方案，均落在本发明的保护范围之内。
Claims (6)
1.一种减少羊抗血清制备过程中羊免疫应激反应的饲料，其特征在于，按重量份包括：玉米50~60份、银杏叶提取物10~20份、陈皮1~3份、柠檬酸0.1~0.5份、松针粉10~30份、番薯藤10~20份、骨粉5~10份、豆渣5~10份、维生素A0.001~0.01份、维生素E0.001~0.01份、维生素C0.001~0.01份、硫酸钴0.01~0.03份。
2.根据权利要求1所述的一种减少羊抗血清制备过程中羊免疫应激反应的饲料，其特征在于，所述银杏叶提取物为银杏叶经乳酸菌发酵3~7天后，烘干粉碎制得。
3.根据权利要求1所述的一种减少羊抗血清制备过程中羊免疫应激反应的饲料，其特征在于，所述乳酸菌为植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌组成的混合菌种。
4.根据权利要求2所述的一种减少羊抗血清制备过程中羊免疫应激反应的饲料，其特征在于，所述混合菌种中植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌的体积比为4-5：3-4：2-3。
5.一种减少羊抗血清制备过程中羊免疫应激反应的饲料的制备方法，其特征在于，包括以下步骤：
（1）将植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌按体积比4-5：3-4：2-3的比例混合制得混合菌种；
（2）将银杏叶洗净、粉碎，加入2倍重量的水，加入银杏叶重量1~3%的混合菌种，温度25~35℃， 密封发酵3~7天，取出烘干，粉碎，得银杏叶提取物；
（3）将玉米50~60份、银杏叶提取物10~20份、陈皮1~3份、柠檬酸0.1~0.5份、松针粉10~30份、番薯藤10~20份、骨粉5~10份、豆渣5~10份、维生素A0.001~0.01份、维生素E0.001~0.01份、维生素C0.001~0.01份、硫酸钴0.01~0.03份混合均匀即得。
6.根据权利要求5所述的一种减少羊抗血清制备过程中羊免疫应激反应的饲料的制备方法，其特征在于，所述步骤（1）中分别取植物乳杆菌、嗜酸乳杆菌、鼠李糖乳杆菌菌种一环在平板上划线，在培养箱中30℃培养24小时；取MRS固体培养基平板所长出的菌落一环接入装有300ml种子培养液的三角瓶中，置于摇床，培养条件：转速80rpm，温度30℃，恒温培养24小时，得各菌种发酵菌种液。
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| CN104771419A (zh) * | 2015-04-20 | 2015-07-15 | 龙岩学院 | 一种银杏叶发酵制剂的制备方法 |
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