CN107279191A - Marigold straw biological organic agricultural chemicals and its application in preventing and treating root-knot nematode - Google Patents
Marigold straw biological organic agricultural chemicals and its application in preventing and treating root-knot nematode Download PDFInfo
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- CN107279191A CN107279191A CN201710326088.XA CN201710326088A CN107279191A CN 107279191 A CN107279191 A CN 107279191A CN 201710326088 A CN201710326088 A CN 201710326088A CN 107279191 A CN107279191 A CN 107279191A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of new marigold straw biological organic agricultural chemicals and its application in preventing and treating root-knot nematode.The marigold straw biological organic agricultural chemicals is by 13 by VT microbial inoculums, marigold stalk, rapeseed dregs and water:8‑10:10‑12:11 15 weight ratio is after normal temperature pile ferments 15 days in container of uncapping, and is obtained by filtering or centrifuge.The organic agricultural chemicals or its dilution, which are sprayed on plant leaf, or impose on plant root can realize effective preventing and treating of root-knot nematode.
Description
Technical field
The present invention relates to control of crop disease field, specifically, be related to marigold straw biological organic agricultural chemicals and its
Prevent and treat the application in root-knot nematode.
Background technology
Tomato root-knot eelworm is important greenhouse vegetable soil-borne disease, according to statistics, annual because tomato root-knot eelworm is caused
The tomato underproduction is up to more than 30%, and tomato root-knot eelworm occurs in that the resistance to the action of a drug to many chemical agents, while chemical agent
High toxicity, easily the brought ecological risk of residual is larger.Therefore it is current agriculture to extract nematocidal active material natural in plant
The inexorable trend of industry production development.From the eighties in last century, pathology of plants man is found that hemlock, marigold, argy wormwood, Huang Hua successively
Chrysanthemum, fennel fiber crops, the metabolite of hemp has good contact efficacy to 2 age root-knot nematodes, has to tomato root-knot eelworm disease good
Good preventive effect, and develop many plant sources and kill line medicament.
The content of the invention
It is an object of the invention to provide a kind of new marigold straw biological organic agricultural chemicals.
It is a further object of the present invention to provide above-mentioned marigold straw biological organic agricultural chemicals answering in preventing and treating root-knot nematode
With.
In order to realize the object of the invention, the marigold straw biological organic agricultural chemicals that the present invention is provided, is by VT microbial inoculums, Wan Shou
Chrysanthemum stalk, rapeseed dregs and water press 1-3:8-10:10-12:11-15 weight ratio normal temperature pile in container of uncapping (such as bucket) is sent out
After ferment 15 days, by filtering or centrifuging, obtained filtrate or supernatant is marigold straw biological organic agricultural chemicals.
Wherein, the VT microbial inoculums include saccharomyces cerevisiae (Saccharomyces cerevisiae) 5.0 × 108~1.0 ×
1010Cfu/mL, Lactobacillus plantarum (Lactobacillus plantarum) 5.0 × 108~1.0 × 1010Cfu/mL, total shape hair
Mould (Mucor racemosus) 1.0 × 102~5.0 × 104Cfu/mL, aspergillus oryzae (Aspergillus oryzae) 1.0 × 103
~5.0 × 104cfu/mL.Preferably, the VT microbial inoculums include saccharomyces cerevisiae 1.0 × 109~6.0 × 109Cfu/mL, plant breast
Bacillus 1.0 × 109~6.0 × 109Cfu/mL, Mucor racemosus 5.0 × 102~5.0 × 103Cfu/mL, aspergillus oryzae 4.0 × 103~
1.0×104cfu/mL.It is highly preferred that the VT microbial inoculums include saccharomyces cerevisiae 1.6 × 109Cfu/mL, Lactobacillus plantarum 1.5 ×
109Cfu/mL, Mucor racemosus 4.1 × 102Cfu/mL, aspergillus oryzae 6.2 × 103cfu/mL。
After the marigold straw biological organic agricultural chemicals of the present invention, each material mixed fermentation 15 days, the carbon nitrogen matter of tunning
Amount is than being 30-35:1.
Preferably, VT microbial inoculums of the invention, marigold stalk, rapeseed dregs and water are by weight 1:10:10:15 mixing hairs
Ferment.
The marigold stalk of the present invention is handled by precrushing：Marigold stalk is crushed using disintegrating machine, it is desirable to particle diameter
Less than 20mm.
The present invention also provides application of the marigold straw biological organic agricultural chemicals in preventing and treating root-knot nematode.Have described
Machine agricultural chemicals or its dilution are sprayed on plant leaf, or impose on plant root；Or prior to seeding, soaked seed with organic agricultural chemicals.
The new marigold straw biological organic agricultural chemicals of the present invention, it is nontoxic to people and animals, it is nuisanceless to environment, with good
Ecological benefits and economic benefit, and find that the medicament is to 2 age root-knot nematodes by indoor virulence test and greenhouse virulence test
The poisoning rate of larva is up to 80% and 81.3%, and effective preventing and treating of root-knot nematode can be achieved, and the medicament can simultaneously serve as blade face
Fertilizer is applied, and nutrition is provided for plant growth.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Unless otherwise specified, embodiment
In the conventional meanses that are well known to those skilled in the art of used technological means, raw materials used is commercial goods.
Marigold stalk is handled by precrushing in following examples：Marigold stalk is crushed using disintegrating machine, it is desirable to
Particle diameter is less than 20mm.The VT microbial inoculums include saccharomyces cerevisiae 1.6 × 109Cfu/mL, Lactobacillus plantarum 1.5 × 109It is cfu/mL, total
Shape Mucor 4.1 × 102Cfu/mL, aspergillus oryzae 6.2 × 103cfu/mL。
The marigold stalk fermentation liquid used in embodiment 4 and 5 is purchased from Beijing Noah agricultural development Co., Ltd.
The marigold straw biological organic agricultural chemicals of embodiment 1 and its preparation
The marigold straw biological organic agricultural chemicals that the present embodiment is provided, is by VT microbial inoculums, marigold stalk, rapeseed dregs and water
By 1:10:10:15 weight ratio in bucket pile ferment 15 days after, tunning (determine tunning in carbon-nitrogen mass ratio
For 30-35:1) by filtering or centrifugation, obtained filtrate or supernatant is marigold straw biological organic agricultural chemicals.
The marigold straw biological organic agricultural chemicals of embodiment 2 and its preparation
The marigold straw biological organic agricultural chemicals that the present embodiment is provided, is by VT microbial inoculums, marigold stalk, rapeseed dregs and water
By 1:8:10:11 weight ratio in bucket pile ferment 15 days after, tunning (determine tunning in carbon-nitrogen mass ratio be
20-22:1) by filtering or centrifugation, obtained filtrate or supernatant is marigold straw biological organic agricultural chemicals.
The marigold straw biological organic agricultural chemicals of embodiment 3 and its preparation
The marigold straw biological organic agricultural chemicals that the present embodiment is provided, is by VT microbial inoculums, marigold stalk, rapeseed dregs and water
By 3:10:12:15 weight ratio in bucket pile ferment 15 days after, tunning (determine tunning in carbon-nitrogen mass ratio
For 38-40:1) by filtering or centrifugation, obtained filtrate or supernatant is marigold straw biological organic agricultural chemicals.
The indoor toxicity experiment of the marigold straw biological organic agricultural chemicals of embodiment 4
The marigold straw biological organic agricultural chemicals stoste 10mL of embodiment 1-3 preparations is separately added into each culture dish,
The sporangiocyst of picking root-knot nematode is placed in biogenic pesticide stoste, agitation, sporangiocyst is sunk under water, blank pair is used as using clear water
According to.And set up VT microbial inoculums, Furadan, marigold stalk fermentation liquid control group.Each processing is in triplicate.The training of sporangiocyst will be equipped with
Foster ware is placed in and is incubated 24h at room temperature, the quantity of the instar larvae of microscopy root-knot nematode 2.It the results are shown in Table 1.
Table 1
| Processing | Nemic death rate |
| The biogenic pesticide stoste of embodiment 1 | 80% |
| The biogenic pesticide stoste of embodiment 2 | 71.3% |
| The biogenic pesticide stoste of embodiment 3 | 67.8% |
| Clear water | 25% |
| VT microbial inoculums (every mu of 2Kg) | 65.5% |
| Furadan (every mu of 1-2Kg) | 64.4% |
| Marigold stalk fermentation liquid (every plant of 20mL) | 65.9% |
The greenhouse toxicity test of the marigold straw biological organic agricultural chemicals of embodiment 5
Be respectively adopted embodiment 1-3 preparation marigold straw biological organic agricultural chemicals stoste, clear water VT microbial inoculums, Furadan and
Marigold stalk fermentation liquid processing, each processing a line sets three repetitions.After tomato field planting, apply sick soil 5kg per a line,
(2 instar larvaes, concentration is about 40000/kg soil), does Disease investigation twice altogether during experiment, once in florescence, once exists
Bear fruit the phase.Botanical pesticide application process is even spraying, makes every effort to using mist uniformly spray during spraying.Botanical pesticide consumption
For every plant of 15mL (continuous using twice), Furadan routinely apply by consumption.It the results are shown in Table 2.
Table 2
| Processing | Nemic death rate |
| The biogenic pesticide stoste of embodiment 1 | 81.3% |
| The biogenic pesticide stoste of embodiment 2 | 76.4% |
| The biogenic pesticide stoste of embodiment 3 | 72.1% |
| Clear water | 27.1% |
| VT microbial inoculums | 70.3% |
| Furadan | 65.5% |
| Marigold stalk fermentation liquid | 68.6% |
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be modified or improved, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (6)
1. marigold straw biological organic agricultural chemicals, it is characterised in that be by 1- by VT microbial inoculums, marigold stalk, rapeseed dregs and water
3:8-10:10-12:11-15 weight ratio in container of uncapping normal temperature pile ferment 15 days after, through filtering or centrifuge, obtain
Filtrate or supernatant be marigold straw biological organic agricultural chemicals；
Wherein, the VT microbial inoculums include saccharomyces cerevisiae (Saccharomyces cerevisiae) 5.0 × 108~1.0 ×
1010Cfu/mL, Lactobacillus plantarum (Lactobacillus plantarum) 5.0 × 108~1.0 × 1010Cfu/mL, total shape hair
Mould (Mucor racemosus) 1.0 × 102~5.0 × 104Cfu/mL, aspergillus oryzae (Aspergillus oryzae) 1.0 × 103
~5.0 × 104cfu/mL；Preferably, the VT microbial inoculums include saccharomyces cerevisiae 1.0 × 109~6.0 × 109Cfu/mL, plant breast
Bacillus 1.0 × 109~6.0 × 109Cfu/mL, Mucor racemosus 5.0 × 102~5.0 × 103Cfu/mL, aspergillus oryzae 4.0 × 103~
1.0×104cfu/mL；It is highly preferred that the VT microbial inoculums include saccharomyces cerevisiae 1.6 × 109Cfu/mL, Lactobacillus plantarum 1.5 ×
109Cfu/mL, Mucor racemosus 4.1 × 102Cfu/mL, aspergillus oryzae 6.2 × 103cfu/mL。
2. organic agricultural chemicals according to claim 1, it is characterised in that after each material mixed fermentation 15 days, tunning
Carbon-nitrogen mass ratio is 30-35:1.
3. organic agricultural chemicals according to claim 2, it is characterised in that VT microbial inoculums, marigold stalk, the weight of rapeseed dregs and water
Amount is than being 1:10:10:15.
4. the organic agricultural chemicals according to claim any one of 1-3, it is characterised in that the particle diameter of the marigold stalk is less than
20mm。
5. application of any one of the claim 1-4 marigold straw biological organic agricultural chemicals in preventing and treating root-knot nematode.
6. application according to claim 5, it is characterised in that the organic agricultural chemicals or its dilution are sprayed at Plant Leaf
On piece, or impose on plant root；Or prior to seeding, soaked seed with organic agricultural chemicals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710326088.XA CN107279191B (en) | 2017-05-10 | 2017-05-10 | Marigold straw bio-organic pesticide and application thereof in preventing and treating root-knot nematodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710326088.XA CN107279191B (en) | 2017-05-10 | 2017-05-10 | Marigold straw bio-organic pesticide and application thereof in preventing and treating root-knot nematodes |
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| Publication Number | Publication Date |
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| CN107279191A true CN107279191A (en) | 2017-10-24 |
| CN107279191B CN107279191B (en) | 2020-06-23 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744259A (en) * | 2017-11-01 | 2019-05-14 | 冠畯农业科技开发有限公司 | Inhibit the natural goods, materials and equipments of nematode |
| CN114557369A (en) * | 2022-03-28 | 2022-05-31 | 赤峰市农牧科学研究所 | Composition for preventing and treating root-knot nematode, preparation method, medicine and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103011922A (en) * | 2012-12-17 | 2013-04-03 | 曲靖康庄肥业有限公司 | Method for preparing bio-organic fertilizer from marigold straws |
-
2017
- 2017-05-10 CN CN201710326088.XA patent/CN107279191B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103011922A (en) * | 2012-12-17 | 2013-04-03 | 曲靖康庄肥业有限公司 | Method for preparing bio-organic fertilizer from marigold straws |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744259A (en) * | 2017-11-01 | 2019-05-14 | 冠畯农业科技开发有限公司 | Inhibit the natural goods, materials and equipments of nematode |
| CN114557369A (en) * | 2022-03-28 | 2022-05-31 | 赤峰市农牧科学研究所 | Composition for preventing and treating root-knot nematode, preparation method, medicine and application thereof |
| CN114557369B (en) * | 2022-03-28 | 2023-05-05 | 赤峰市农牧科学研究所 | Composition for preventing and treating root-knot nematode, and preparation method, medicine and application thereof |
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| Publication number | Publication date |
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| CN107279191B (en) | 2020-06-23 |
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