CN107267389A - A kind of cell expansion apparatus - Google Patents
A kind of cell expansion apparatus Download PDFInfo
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- CN107267389A CN107267389A CN201710730044.3A CN201710730044A CN107267389A CN 107267389 A CN107267389 A CN 107267389A CN 201710730044 A CN201710730044 A CN 201710730044A CN 107267389 A CN107267389 A CN 107267389A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/14—Pressurized fluid
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Bioinformatics & Cheminformatics (AREA)
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Abstract
The invention discloses a kind of cell expansion apparatus, including culture tank, tank mouth is set in the center of top of tank body；The cross section of tank body top inner surface is gradually reduced from side-walls to tank mouth, and is gradually risen from side wall to the junction of tank mouth；The side wall of piston body and the inner surface side wall of tank body are in sealing contact, and piston body can make translation lifting along the inwall of tank body, can discharge nutrient solution at tank mouth during rising, and piston body upper surface can be contacted with the top surface laminating of the inner surface of tank body.Rotary connector is fixed on the bottom of tank body, for connecting rotary power unit, to drive tank body to rotate.Tank body is driven to rotate by rotary connector, the cell in nutrient solution is different because of quality, the cell category produced under the action of the centrifugal force in layering, different layers is different；Because the top surface of tank body is gradually reduced from bottom to top, during promotion, internal layer liquid is first discharged, and is discharged after outer layer liquid, and relatively pure cell or other liquid is obtained by layering.
Description
Technical field
The present invention relates to cell preparation techniques field, a kind of cell expansion apparatus is further related to.
Background technology
Patient needs to remove tumour cell over the course for the treatment of, and kill tumour cell is not selective, while can also kill
Dead substantial amounts of healthy cell, because individual physique level is not quite similar, the reproduction speed of some healthy cells in patient body does not have
The reproduction speed of tumour cell is fast, and while final result is that tumour cell elimination is finished, useful healthy cell is also killed
Light, patient finally still can be in face of death.To solve above-mentioned problem, a kind of new tumor therapeuticing method is occurred in that：
Cell therapy, its main thought is exactly to extract healthy cell sample from the patient, by external device culture, is copied big
Patient's cell of amount, is then input in patient body, helps patient to get well again.
Cell culture is a kind of method for the research zooblast behavior founded earlier 1900s, takes out thin from in-vivo tissue
Growing environment in born of the same parents, analogue body, breeds cell growth under proper temperature, acid-base value, a kind of sterile and nutritional condition, ties up
Hold its 26S Proteasome Structure and Function.Prepared by cell is the whole product of cell for producing high-purity with cell culture protocol, for scientific research with
The process of clinical field.
The culture and preparation of cell need to meet appropriate external condition, in the cell augmentation apparatus generally used at present,
Culture tank is common bottle, its shape causes centrifugation, after hierarchical operations, it is necessary to first extract useful cell, then fall again useless
Useful cell, is finally refunded culture tank by liquid again, and cell is passed in and out back and forth, is easily damaged, and inefficiency.
The content of the invention
The present invention provides a kind of cell expansion apparatus, it is not necessary to pour out waste liquid, keeps cell pure, efficiency high and will not damage
Cell, concrete scheme is as follows：
A kind of cell expansion apparatus, including culture tank, the culture tank include：
Tank body, inside is used to contain nutrient solution, and its center of top sets tank mouth, the cross section of the tank body top inner surface
It is gradually reduced, and is gradually risen from side wall to the junction of the tank mouth from side-walls to the tank mouth；
Piston body, the inner surface side wall of its side wall and the tank body is in sealing contact, and the piston body can be along the tank body
Inwall make translation lifting；The piston body upper surface can be contacted with the top surface laminating of the tank inner surface；
Rotary connector, is fixed on the bottom of the tank body, for connecting rotary power unit, to drive the tank body to turn
It is dynamic.
Alternatively, in addition to the cover that is fixedly installed, set can connect exterior line to connect mouth on the cover；It is described
The inwall of cover is tightly connected with the tank mouth, and the cover keeps rotating and is connected with the tank mouth by bearing.
Alternatively, the tank mouth is step-like column structure, step structure that can be with the inner surface of the cover
Coordinate assembling；Sealing gasket is set with the tank mouth to keep sealing with the cover.
Alternatively, the sealing gasket is at least provided with the fin that tapered tilting is set on two, and the sealing gasket；Two
The fin on the sealing gasket is oppositely arranged, on the cascaded surface for being crimped on the cover.
Alternatively, the week of the piston body sets up multiple mounting grooves, is sealed for installing with the tank inner surface
The sealing ring of contact；The top surface integrated setting of the tank mouth and the tank body, and by clamp ring and the tank body
Wall is threadably secured connection.
Alternatively, in addition to for being set with the tophan box of the culture tank is fixed, the tophan box includes：
Can body, is set in the outside of the culture tank, is fixedly connected with the cover；
Outer radiator, is arranged at the outer surface of the can body, sets multigroup fin；
Inner radiator, is arranged at the inner surface of the can body, sets multigroup fin, and be arranged at the outer radiator
The relative position of the can body；
Semiconductor refrigerating module, is arranged between the outer radiator and the inner radiator；
Temperature sensor, for detecting the fluid temperature inside the culture tank；
Motor is centrifuged, the bottom of the can body is arranged at, can drive and be rotated with the tank body.
Alternatively, in addition to for connecting air inlet and air outlet inside and outside the can body, air-flow enters from the air inlet
Enter inside the can body, and discharged through the air outlet.
Alternatively, the air inlet is vertically arranged two, and air outlet sets one, and the air outlet is arranged at described in two
Between air inlet；Wind guide tank is set on the can body, and the air inlet and the air outlet are connected on the wind guide tank, described
Baffle plate is set in wind guide tank, and for the air inlet and the air outlet is mutually isolated, wind enters from the air inlet to be surround
The can body is discharged for one week from the air outlet.
Alternatively, in addition to the removable cover that is arranged at the top of the can body, the centre of the removable cover is opened up for fixing
The relief hole of the cover.
Alternatively, the removable cover includes two and half domes for being spliced to form complete lid, and two and half domes are close to each other
A lateral edges set Rotational Cylindrical, two and half domes is horizontally rotated opening；The position relative with the Rotational Cylindrical is set
Lock, for two pieces of semicircle cover locks to be fastened.
The invention discloses a kind of cell expansion apparatus, including culture tank, culture tank specifically includes tank body, piston body, rotation
Turn the structures such as connector, the inside of tank body is used to contain nutrient solution, and tank mouth is set in the center of top of tank body, for outwards exporting
Nutrient solution；The cross section of tank body top inner surface is gradually reduced from side-walls to tank mouth, and from side wall to the junction of tank mouth by
Edge up height, when nutrient solution is moved upwards under the promotion of piston body, nutrient solution is finally collected at tank mouth and discharges；Piston body
The inner surface side wall of side wall and tank body is in sealing contact, and piston body can make translation lifting along the inwall of tank body, will can be trained during rising
Nutrient solution is discharged at tank mouth, and piston body upper surface can be contacted with the top surface laminating of the inner surface of tank body, so as to completely will
Nutrient solution is discharged.Rotary connector is fixed on the bottom of tank body, for connecting rotary power unit, to drive tank body to rotate.
When needing separation cell, tank body is driven to rotate by rotary connector, the cell in nutrient solution is different because of quality,
The cell category produced under the action of the centrifugal force in layering, different layers is different；Now control piston body rises, and nutrient solution is upward
Promote, because the top surface of tank body is gradually reduced from bottom to top, during promotion, innermost layer liquid is discharged at first, more outer
The more late discharge of liquid of layer, so as to obtain relatively pure cell or other liquid.
The cell expansion apparatus of the present invention, only sets a tank mouth to be used for discharge opeing, therefore the layering produced by centrifugal force
Effect realizes the classification of cell, and cell and waste liquid are discharged from tank mouth, cell is in all the time in sealed environment, it is to avoid cell
It is contaminated.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation of culture tank；
Fig. 2 is the assembling explosive view between tank mouth and cover；
Fig. 3 is the structural representation of tophan box；
Fig. 4 A are a kind of concrete structure diagram of wind guide tank；
Fig. 4 B are the perspective structure figure of wind guide tank.
Including：
Culture tank 1, tank body 11, tank mouth 12, piston body 13, mounting groove 131, clamp ring 14, rotary connector 15, cover 2,
Bearing 21, sealing gasket 22, fin 221, connect mouth 23, tophan box 3, can body 31, outer radiator 32, inner radiator 33, TEMP
Device 34, centrifugation motor 35, air inlet 36, air outlet 37, baffle plate 38, removable cover 39, relief hole 391, Rotational Cylindrical 392, lock
393。
Embodiment
The core of the present invention is to provide a kind of cell expansion apparatus, it is not necessary to pour out waste liquid, keeps cell pure, efficiency high
And will not damaging cells.
In order that those skilled in the art more fully understands technical scheme, below in conjunction with accompanying drawing and specifically
Embodiment, to the present invention cell expansion apparatus explanation is described in detail.
Cell expansion apparatus provided by the present invention include culture tank 1, culture tank 1 further comprise tank body 11, tank mouth 12,
The structures such as piston body 13, rotary connector 15, tank body 11 is provided with columnar side wall, and top surface is sealed with side wall respectively with bottom surface to be connected
Connect, make tank body 11 is internally formed sealed cavity.
As shown in figure 1, being the structural representation of culture tank.The inside of tank body 11 be used for contain nutrient solution, its top namely
The center of top surface sets tank mouth 12, the connection tank body 11 of tank mouth 12 it is inside and outside, the cross section of the top inner surface of tank body 1 from side-walls to
Tank mouth 12 is gradually reduced, and is gradually risen from side wall to the junction of tank mouth 12；Preferably, top surface should be set to circular cone
Face, tank mouth 12 is arranged on the summit of taper, certainly, the present invention be also not limited to it is coniform, can also be for other from bottom to top gradually
The structure of contracting, no longer illustrates one by one herein, and these specific forms should be all included within protection scope of the present invention.
Piston body 13 is arranged on the inside of tank body 11, and the inner surface side wall of its side wall and tank body 11 is in sealing contact, makes culture
Liquid is in the cavity between the upper surface of piston body 13 and tank body 11, and piston body 13 can make translation along the inwall of tank body 11 and rise
Drop, because piston body 13 is contacted with the inner wall sealing of tank body 11, therefore nutrient solution is promoted by piston body 13 move up and down all the time.It is living
The upper surface of cock body 13 is identical with the top shape and size of the inner surface of tank body 11, when piston body 13 rises to top, living
Cock body 13 is contacted with the top surface laminating of the inner surface of tank body 11, so that nutrient solution be extruded completely.
Preferably, the piston body 13 in the present invention is realized by air pressure and lifted, lower section and the outside air phase of piston body 13
It is logical, when toward connect pressurizeed on mouth 23 when, piston body 13 is moved down, and realizes that liquid is poured into, when toward connect take out negative pressure on mouth 23 when, piston body 13
Move up, realize that liquid is extracted out.Certainly, piston body 13 also can set lifting structure using other type of drive, such as bottom.
Rotary connector 15 is fixed on the bottom of tank body 11, for connecting rotary power unit, to drive tank body 11 to rotate,
Rotary power unit can drive tank body 11 to rotate when rotating, nutrient solution also can concomitant rotation, when reaching specific rotating speed, cultivate
The materials such as the cell in liquid are layered because of quality difference, quality greatly positioned at outer layer.
When detachment is desired, rotate tank body 11, reach cell after corresponding rotating speed and be layered, now control piston body 13
Motion upwards, promotes nutrient solution to be discharged from the tank mouth 12 of top, because the top surface of tank body 11 is coniform, therefore in innermost layer
Liquid can first be discharged, leave outer layer liquid, discharge part is the waste liquid for containing substantially no cell, and the liquid left is comprising big
Part cell, adds new nutrient solution to tank body 11 and continues to cultivate, incubation is maintained at the suitable condition of cell growth.
The cell expansion apparatus of the present invention, only sets a tank mouth 12 to be used for discharge opeing, and the layering produced by centrifugal force is imitated
The classification of the existing cell of fruit, cell and waste liquid are discharged from tank mouth, and the extraction efficiency of nutrient solution and cell is higher；Cell is located all the time
In sealed environment, it is to avoid cell is contaminated；In addition, selecting cell by the way of centrifugation layering, it can obtain relative
Pure target cell.
On the basis of such scheme, in order to be more preferably connected with exterior line, cell expansion apparatus of the invention also includes
Cover 2, cover 2 remains stationary as to be fixedly installed when tank body 11 rotates.Connecing for exterior line can be connected by being set on cover 2
Mouth 23, inwall and the tank mouth 12 of cover 2 are tightly connected, and the nutrient solution discharged from tank mouth 12 being capable of connecing in mouth 23 from cover 2
Discharge.Cover 2 is kept rotating and is connected with tank mouth 12 by bearing 21, and the outer ring of bearing 21 is fixed in the inner chamber of cover 2,
The inner ring of bearing 21 is fixed on outside tank mouth 12, is partly contacted between the outer surface of tank mouth 12 and the inwall of cover 2, contact
Portions airtight.Cover 2 remains static during work, only rotates tank body 11, and the layering of tank inner cell can be achieved.
Specifically, tank mouth 12 is step-like column structure, stepped knot that can be with the inner surface of cover 2 in the present invention
Structure coordinates assembling；Be set with sealing gasket 22 and cover 2 on tank mouth 12 to keep sealing, can be set multilayer gasket, protection nutrient solution without
Method flows out from gap between the two.
The design of cover and tank mouth causes cell expansion module relatively independent in the present invention, can easily access and appoint
What existing automatic culture device of cell, it is not necessary to change other apparatus structures and the woven hose being connected with culture tank etc.；It is convenient
Plant maintenance and fault diagnosis.
In order that keeping good sealing between tank mouth 12 and cover 2, sealing gasket 22 is at least provided with two, and sealing gasket 22
The fin 221 of the upper tapered tilting of setting, fin 221 is the cone-shaped structure for protruding from the body of sealing gasket 22, and fin 221 has
Fin 221 on elasticity, two sealing gaskets 22 is oppositely arranged, and sets the groove of annular to install sealing gasket on the inwall of cover 2
22, this groove one step surface of formation, the inside of cover 2 is stepped in itself, and another sealing gasket 22 is crimped on ladder, namely
The fin 221 of two sealing gaskets 22 is oppositely arranged, and is crimped on respectively on two cascaded surfaces.Using close with fin 221 up and down
Packing 22, it is possible to achieve normal pressure, superpressure, the static state under negative pressure operating mode, dynamic sealing.
Multiple mounting grooves 131 are set up the week of piston body 13, for installing sealing ring, sealing ring and the inner surface of tank body 11
It is in sealing contact.Fabricate for convenience, the top surface integrated setting of tank mouth 12 and tank body 11, as shown in Fig. 2 for tank mouth 12 with
Assembling explosive view between cover 2, the surrounding of top surface is threadably secured by clamp ring 16 with the side wall of tank body 11 to be connected.Lock
Collar 16 is cyclic structure, and cross section is L-shaped, is threaded in upright side walls, top edge can be then pressed on by horizontal segment
On the side wall of tank body 11, so as to realize sealing.
In any of the above-described technical scheme and its on the basis of being mutually combined, present invention additionally comprises for being set with fixed culture tank
1 tophan box 3, as shown in figure 3, being the structural representation of tophan box；Tophan box 3 is located at outside culture tank 1, can drive culture tank
1 rotates.Tophan box 3 includes can body 31, outer radiator 32, inner radiator 33, semiconductor refrigerating module, temperature sensor
34th, the structure such as centrifugation motor 35.
Can body 31 is tubular, is set in the outside of culture tank 1, is fixedly connected with cover 2；Outer radiator 32 is arranged at can body 31
Outer surface, multigroup fin is set, and outer radiator 32 is made up of the good material of heat conductivility, increase and the contact surface of air
Product, accelerates heat conduction；Inner radiator 33 is arranged at the inner surface of can body 31, in the gap between can body 31 and culture tank 1, if
Multigroup fin is put, similar to the structure of outer radiator 32, inner radiator 33 is arranged at the relative of can body 31 with outer radiator 32
Position, inner radiator 33 accelerates to absorb or diffusion heat, makes the fast cooling of culture tank 1 or heating, inner radiator 33 and outer radiating
Device 32 is close to each other, with the conduction of accelerated heat.
Semiconductor refrigerating module is arranged between outer radiator 32 and inner radiator 33；Semiconductor refrigerating module is a kind of piece
The electronic component of shape, with huyashi-chuuka (cold chinese-style noodles) and hot face Liang Ge faces, when applying forward voltage, hot face heating, huyashi-chuuka (cold chinese-style noodles) refrigeration；When to this
When component applies backward voltage, huyashi-chuuka (cold chinese-style noodles) heating, hot face refrigeration.Two panels inner radiator 33 is respectively mounted on can body 31, outside two panels
Radiator 32, totally four, a piece of outer radiator 32 is a pair with a piece of inner radiator, and each pair radiator is sandwiched between semiconductor system
Chill block (not shown), the heat of semiconductor refrigerating module is faced in tank.
Temperature sensor 34 is arranged on can body 31, for detecting the fluid temperature inside culture tank 1, can use infrared temperature
Spend inductor.Centrifugation motor 35 is arranged at the bottom of can body 31, and tank body 11 can be driven to rotate.During work, the placement of culture tank 1
Connector 15 is connected with centrifuging the chuck of motor 35 on tophan box 3, and cover 2 is fixedly connected with tophan box 3, and it connects mouth 23 and connects transfusion
Pipe.Tank body 11, tank mouth 12, the inner ring of bearing 21, sealing gasket 22 of culture tank 1 etc. is driven to turn together when centrifuging motor 35 and rotating
Dynamic, because cover 2 and can body 31 keep fixing, cover 2 is not rotated, and it is also not to be connected on the woven hose that cover 2 connect on mouth 23
Rotate, so, just realize tank body 11 and rotate, cover 2 and woven hose are static.Simultaneously as the wing of upper and lower two sealing gaskets
Piece 221 is close on the small stair of cover 2, when toward liquid filling body in tank body 11, and the pressure in woven hose is more than outside air
Pressure, the fin 221 that upper sealing gasket is set down can be close to the step of cover 2, realize that excess air pressure is sealed；When drawing liquid body outward
When, the interior pressure of woven hose is less than external air pressure, and the fin 221 that lower seal pad is set upward is just close to the platform of cover 2
Rank, realizes means of press seals of doing something in a fit of pique.
Also include being used to connect the air inlet 36 and air outlet 37 inside and outside can body 31, air-flow enters can body 31 from air inlet 36
Inside, and discharged through air outlet 37.Air inlet 36 and air outlet 37 set docking structure respectively, for connecting blast pipe and air-out
Pipe.
The position and quantity of air inlet 36 and air outlet 37 can be adjusted correspondingly according to requirements, it is preferable that this
In invention air inlet 36 be vertically arranged setting two, air outlet 37 set one, air outlet 37 be arranged at two air inlets 36 it
Between, two air inlets 36 and an air outlet 37 are vertically arranged setting.
Wind guide tank is set on can body 31, is a kind of concrete structure diagram of wind guide tank as shown in Figure 4 A, wind guide tank is to be arranged at
Hollow sandwich on can body 31, air inlet 36 and air outlet 37 are connected on wind guide tank, and baffle plate 38 is set in wind guide tank, for inciting somebody to action
Space interval is separated between can body 31 and tank body 11, as shown in Figure 4 B, is the perspective structure figure of wind guide tank, baffle plate 38 is in multiple curved
The S type structures of folding, for air inlet 36 and air outlet 37 is mutually isolated, wind from air inlet 36 enter can body 31 and tank body 11 it
Between gap in, due to the separation of baffle plate 38, wind can only be discharged for 31 1 weeks around can body from air outlet 37.The air circulation of entrance
Discharge within one week, heat transfer effect is more abundant.
By two air inlets 36 and an air outlet 37, totally three structures are divided into two parts, air intake for the inner side of wind guide tank
Mouthfuls 36 towards the right side, and air outlet 37 is towards a left side.During work, the external closed pneumatic source of air inlet 36, it is ensured that always there is gas to enter from air inlet 36,
Go out from air outlet 37, with heat-insulated and sealing function, it is ensured that do not exchanged with ambient atmos hair body heat.When the inspection of infrared temperature inductor
When measuring the fluid temperature in culture tank 1 less than target temperature, just apply forward voltage in refrigeration module, allow inner radiator to send out
Heat, and keep temperature in whole tank uniform by air flow；The liquid temperature in culture tank 1 is detected when infrared temperature inductor
When degree is higher than target temperature, just applies backward voltage in refrigeration module, allow inner radiator to freeze, to keep liquid in culture tank 1
Temperature is target temperature, reaches the purpose of constant temperature, samming.
Present invention additionally comprises the removable cover 39 for being arranged at the top of can body 31, the centre of removable cover 39 is opened up for fixing cover
2 relief hole 391.Removable cover 39 movable can be opened, and culture tank 1 is picked and placeed to facilitate.In the insertion relief hole 391 of cover 2, and by
Relief hole 391, which is realized, to be fixed, and stable support force is provided to the circumferential of cover 2.
Specifically, removable cover 39 includes two and half domes for being spliced to form complete lid, and each semicircle is covered to be set respectively
Semicircular perforate, can form relief hole 391；Rotational Cylindrical is set at two and half domes side edge-corner close to each other
392, two and half domes is horizontally rotated opening, certainly, the concrete structure and unfolding mode of removable cover 39 in the present invention
Corresponding adjustment can be made and improved, as long as can play fixed cover 2 realizes discharge, these improvement should be included in
Within protection scope of the present invention.Lock 393 is set in the position relative with Rotational Cylindrical 392, for by two pieces of semicircle cover lockings
It is fixed, it can be achieved the clamping of cover 2 after two pieces of semicircle cover lockings.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using the present invention.
A variety of modifications to these embodiments will be apparent for those skilled in the art, as defined herein
General Principle, can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, this hair
It is bright to be not intended to be limited to the embodiments shown herein, and be to fit to and principles disclosed herein and features of novelty phase
Consistent most wide scope.
Claims (10)
1. a kind of cell expansion apparatus, it is characterised in that including culture tank (1), the culture tank (1) includes：
Tank body (11), inside is used to contain nutrient solution, and its center of top sets tank mouth (12), tank body (11) top inner surface
Cross section be gradually reduced from side-walls to the tank mouth (12), and gradually risen from side wall to the junction of the tank mouth (12)
It is high；
Piston body (13), the inner surface side wall of its side wall and the tank body (11) is in sealing contact, and the piston body (13) being capable of edge
The inwall of the tank body (11) makees translation lifting；Piston body (13) upper surface can be with the tank body (11) inner surface top
Face laminating contact；
Rotary connector (15), is fixed on the bottom of the tank body (11), for connecting rotary power unit, to drive the tank
Body (11) is rotated.
2. cell expansion apparatus according to claim 1, it is characterised in that described also including the cover (2) being fixedly installed
Set can connect exterior line to connect mouth (23) on cover (2)；The inwall of the cover (2) is sealed with the tank mouth (12) to be connected
Connect, the cover (2) keeps rotating and is connected with the tank mouth (12) by bearing (21).
3. cell expansion apparatus according to claim 2, it is characterised in that the tank mouth (12) is step-like column knot
Structure, can coordinate with the step structure of the inner surface of the cover (2) and assemble；Sealing gasket (22) is set with the tank mouth (12)
Keep sealing with the cover (2).
4. cell expansion apparatus according to claim 3, it is characterised in that the sealing gasket (22) at least provided with two,
And the fin (221) of tapered tilting is set on the sealing gasket (22)；The fin on two sealing gaskets (22)
(221) it is oppositely arranged, on the cascaded surface for being crimped on the cover (2).
5. cell expansion apparatus according to claim 1, it is characterised in that setting up in week for the piston body (13) is more
Individual mounting groove (131), for installing and the tank body (11) inner surface sealing ring in sealing contact；The tank mouth (12) with it is described
The top surface integrated setting of tank body (11), and company is threadably secured by the inwall of clamp ring (16) and the tank body (11)
Connect.
6. the cell expansion apparatus according to any one of claim 2 to 5, it is characterised in that also including for being set with fixation
The tophan box (3) of the culture tank (1), the tophan box (3) includes：
Can body (31), is set in the outside of the culture tank (1), is fixedly connected with the cover (2)；
Outer radiator (32), is arranged at the outer surface of the can body (31), sets multigroup fin；
Inner radiator (33), is arranged at the inner surface of the can body (31), sets multigroup fin, and with the outer radiator
(32) it is arranged at the relative position of the can body (31)；
Semiconductor refrigerating module, is arranged between the outer radiator (32) and the inner radiator (33)；
Temperature sensor (34), the fluid temperature internal for detecting the culture tank (1)；
Motor (35) is centrifuged, the bottom of the can body (31) is arranged at, can drive and be rotated with the tank body (11).
7. cell expansion apparatus according to claim 6, it is characterised in that also including for connecting in the can body (31)
Outer air inlet (36) and air outlet (37), air-flow enters the can body (31) inside from the air inlet (36), and through described
Air outlet (37) is discharged.
8. cell expansion apparatus according to claim 7, it is characterised in that the air inlet (36) is vertically arranged two,
Air outlet (37) sets one, and the air outlet (37) is arranged between two air inlets (36)；On the can body (31)
Wind guide tank is set, and the air inlet (36) and the air outlet (37) are connected on the wind guide tank, set in the wind guide tank
Baffle plate (38), for the air inlet (36) and the air outlet (37) is mutually isolated, wind enters from the air inlet (36)
Discharged around the can body from the air outlet (37) within (31) one weeks.
9. cell expansion apparatus according to claim 8, it is characterised in that also including being arranged at the top of the can body (31)
Removable cover (39), the centre of the removable cover (39) opens up the relief hole (391) for fixing the cover (2).
10. cell expansion apparatus according to claim 9, it is characterised in that the removable cover (39) includes being spliced to form
Two and half domes of complete lid, a two and half domes lateral edges close to each other set Rotational Cylindrical (392), make two and half domes
Opening can be horizontally rotated；The position relative with the Rotational Cylindrical (392) sets lock (393), for by two pieces of semicircle cover locks
Fasten.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710730044.3A CN107267389B (en) | 2017-08-23 | 2017-08-23 | Cell amplification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710730044.3A CN107267389B (en) | 2017-08-23 | 2017-08-23 | Cell amplification device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107267389A true CN107267389A (en) | 2017-10-20 |
| CN107267389B CN107267389B (en) | 2020-06-19 |
Family
ID=60076431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710730044.3A Active CN107267389B (en) | 2017-08-23 | 2017-08-23 | Cell amplification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107267389B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004031A (en) * | 2019-04-26 | 2019-07-12 | 南京比瑞生物科技有限公司 | Centrifugal classification bioreactor and micro-imaging method for separating |
| CN110016437A (en) * | 2019-04-26 | 2019-07-16 | 南京比瑞生物科技有限公司 | Vertical rotating tank bioreactor for large-scale culture cell |
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| Publication number | Publication date |
|---|---|
| CN107267389B (en) | 2020-06-19 |
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