CN107267389B

CN107267389B - Cell amplification device

Info

Publication number: CN107267389B
Application number: CN201710730044.3A
Authority: CN
Inventors: 张锴; 赵明; 宋志兵; 刘肖琳
Original assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Current assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Priority date: 2017-08-23
Filing date: 2017-08-23
Publication date: 2020-06-19
Anticipated expiration: 2037-08-23
Also published as: CN107267389A

Abstract

The invention discloses a cell amplification device, which comprises a culture tank, wherein a tank nozzle is arranged in the center of the top of a tank body; the cross section of the inner surface of the top of the tank body is gradually reduced from the side wall to the tank nozzle and gradually increased from the side wall to the connection part of the tank nozzle; the side wall of the piston body is in sealing contact with the side wall of the inner surface of the tank body, the piston body can be moved horizontally and lifted along the inner wall of the tank body, the culture solution can be discharged from the tank nozzle during lifting, and the upper surface of the piston body can be in contact with the top surface of the inner surface of the tank body in an attaching manner. The rotary connecting piece is fixed at the bottom of the tank body and is used for connecting the rotary power device so as to drive the tank body to rotate. The tank body is driven to rotate through the rotary connecting piece, cells in the culture solution are layered under the action of centrifugal force due to different masses, and the types of the cells in different layers are different; because the top surface of the tank body is gradually reduced from bottom to top, in the pushing process, the inner layer liquid is discharged firstly, the outer layer liquid is discharged later, and relatively pure cells or other liquids are obtained through layering.

Description

Cell amplification device

Technical Field

The invention relates to the technical field of cell preparation, and further relates to a cell amplification device.

Background

In the treatment process of a patient, tumor cells need to be eliminated, the tumor cells are killed without selectivity, a large number of healthy cells can be killed, the healthy cells in the body of some patients are not regenerated at high speed due to different individual constitution levels, and the final result is that the useful healthy cells are also killed while the tumor cells are eliminated, and the patient can still face death. In order to solve the existing problems, a novel tumor treatment method is provided: the cell therapy mainly aims at extracting a healthy cell sample from a patient, culturing the cell sample by an in-vitro device to reproduce a large amount of patient cells, and then inputting the cell sample into the patient to help the patient to recover the health.

Cell culture is a method for researching animal cell behaviors, which is established in the beginning of the twentieth century, and is characterized in that cells are taken out from in vivo tissues, the in vivo growth environment is simulated, and the cells are grown and propagated under the conditions of proper temperature, pH value, sterility and one kind of nutrition, so that the structure and the function of the cells are maintained. Cell preparation is a process of preparing a high-purity cell final product by using a cell culture scheme, and is used in scientific research and clinical fields.

The culture and preparation of cells need to meet proper external conditions, in the currently widely adopted cell amplification equipment, a culture tank is a common bottle, after centrifugation and layering operations are caused by the shape of the common bottle, useful cells need to be extracted firstly, then waste liquid is poured, finally the useful cells are poured back to the culture tank, the cells come in and go out back and forth, damage is easy to occur, and the efficiency is low.

Disclosure of Invention

The invention provides a cell amplification device, which can keep cells pure, has high efficiency and can not damage the cells without pouring waste liquid, and the specific scheme is as follows:

a cell expansion device comprising a culture tank, the culture tank comprising:

the culture medium tank comprises a tank body, a tank opening and a tank cover, wherein the tank body is used for containing culture medium, the center of the top of the tank body is provided with the tank opening, the cross section of the inner surface of the top of the tank body is gradually reduced from the side wall to the tank opening, and the cross section of the inner surface of the top of the tank body is gradually increased from the side wall to the joint;

the side wall of the piston body is in sealing contact with the side wall of the inner surface of the tank body, and the piston body can perform translational lifting along the inner wall of the tank body; the upper surface of the piston body can be in contact with the top surface of the inner surface of the tank body in a fitting manner;

and the rotary connecting piece is fixed at the bottom of the tank body and is used for connecting a rotary power device so as to drive the tank body to rotate.

Optionally, the device further comprises a fixedly arranged tank cover, wherein a nozzle capable of being connected with an external pipeline is arranged on the tank cover; the inner wall of the tank cover is connected with the tank nozzle in a sealing mode, and the tank cover is rotatably connected with the tank nozzle through a bearing.

Optionally, the can mouth is of a stepped columnar structure and can be matched and assembled with the stepped structure on the inner surface of the can cover; the can mouth is sleeved with a sealing gasket to keep sealing with the can cover.

Optionally, at least two sealing gaskets are arranged, and a conical and tilted fin is arranged on each sealing gasket; the two fins on the sealing gaskets are oppositely arranged and are pressed on the stepped surface of the tank cover.

Optionally, a plurality of mounting grooves are formed in the circumferential direction of the piston body and used for mounting a sealing ring which is in sealing contact with the inner surface of the tank body; the jar mouth with the top surface integration of the jar body sets up to through the check lock circle with the inner wall of the jar body passes through screw thread fixed connection.

Optionally, the culture tank further comprises a centrifuge tank for fixing the culture tank in a sleeving manner, wherein the centrifuge tank comprises:

the tank body is sleeved on the outer side of the culture tank and fixedly connected with the tank cover;

the outer radiator is arranged on the outer surface of the tank body and is provided with a plurality of groups of radiating fins;

the inner radiator is arranged on the inner surface of the tank body, is provided with a plurality of groups of radiating fins, and is arranged at the position opposite to the outer radiator on the tank body;

the semiconductor refrigeration module is arranged between the outer radiator and the inner radiator;

a temperature sensor for detecting the temperature of the liquid inside the culture tank;

the centrifugal motor is arranged at the bottom of the tank body and can drive the tank body to rotate.

Optionally, the air-conditioning tank further comprises an air inlet and an air outlet, the air inlet and the air outlet are used for communicating the inside and the outside of the tank body, and the air flow enters the inside of the tank body from the air inlet and is discharged through the air outlet.

Optionally, two air inlets are vertically arranged, one air outlet is arranged, and the air outlet is arranged between the two air inlets; the air guide tank is arranged on the tank body, the air inlet and the air outlet are connected to the air guide tank, a baffle is arranged in the air guide tank and used for isolating the air inlet and the air outlet from each other, and air enters from the air inlet and surrounds the tank body for a circle and is discharged from the air outlet.

Optionally, the tank cover further comprises a movable cover arranged at the top of the tank body, and a discharge hole for fixing the tank cover is formed in the middle of the movable cover.

Optionally, the movable cover comprises two semicircular covers spliced to form a complete cover body, and a rotating column is arranged at the edge of one side of each semicircular cover, which is close to each other, so that the two semicircular covers can be opened in a horizontal rotating manner; and a lock catch is arranged at the position opposite to the rotating column and used for locking and fixing the two semicircular covers.

The invention discloses a cell amplification device, which comprises a culture tank, wherein the culture tank specifically comprises a tank body, a piston body, a rotary connecting piece and other structures, the interior of the tank body is used for containing a culture solution, and a tank nozzle is arranged in the center of the top of the tank body and used for outputting the culture solution outwards; the cross section of the inner surface of the top of the tank body is gradually reduced from the side wall to the tank nozzle, and gradually rises from the side wall to the joint of the tank nozzle, and when the culture solution moves upwards under the pushing of the piston body, the culture solution is finally collected at the tank nozzle and is discharged; the side wall of the piston body is in sealing contact with the side wall of the inner surface of the tank body, the piston body can be moved horizontally and lifted along the inner wall of the tank body, the culture solution can be discharged from the tank nozzle when the piston body is lifted, and the upper surface of the piston body can be in contact with the top surface of the inner surface of the tank body in an attaching mode, so that the culture solution can be completely discharged. The rotary connecting piece is fixed at the bottom of the tank body and is used for connecting the rotary power device so as to drive the tank body to rotate.

When cells need to be separated, the tank body is driven to rotate through the rotary connecting piece, the cells in the culture solution are layered under the action of centrifugal force due to different masses, and the cells in different layers are different in types; at the moment, the piston body is controlled to rise to push the culture solution upwards, and as the top surface of the tank body is gradually reduced from bottom to top, in the pushing process, the liquid at the innermost layer is discharged firstly, and the liquid closer to the outer layer is discharged later, so that relatively pure cells or other liquids are obtained.

According to the cell amplification device, only one tank nozzle is arranged for discharging liquid, so that the cells are classified by the layering effect generated by centrifugal force, and the cells and waste liquid are discharged from the tank nozzle, so that the cells are always in a sealed environment, and the cells are prevented from being polluted.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of the structure of a culture tank;

FIG. 2 is an exploded view of the assembly between the spout and the lid;

FIG. 3 is a schematic structural view of a centrifuge tank;

FIG. 4A is a detailed structural view of the air guiding groove;

fig. 4B is a perspective view of the air guide duct.

Which comprises the following steps:

the culture tank comprises a culture tank 1, a tank body 11, a tank mouth 12, a piston body 13, a mounting groove 131, a locking ring 14, a rotary connecting piece 15, a tank cover 2, a bearing 21, a sealing gasket 22, fins 221, a connecting mouth 23, a centrifugal tank 3, a tank body 31, an outer radiator 32, an inner radiator 33, a temperature sensor 34, a centrifugal motor 35, an air inlet 36, an air outlet 37, a baffle plate 38, a movable cover 39, a discharge hole 391, a rotating column 392 and a lock 393.

Detailed Description

The core of the present invention is to provide a cell expansion apparatus which can maintain the purity of cells without pouring out waste liquid, has high efficiency, and does not damage cells.

In order to make those skilled in the art better understand the technical solution of the present invention, the cell expansion device of the present invention will be described in detail with reference to the accompanying drawings and specific embodiments.

The cell amplification device provided by the invention comprises a culture tank 1, wherein the culture tank 1 further comprises a tank body 11, a tank nozzle 12, a piston body 13, a rotary connecting piece 15 and other structures, the tank body 11 is provided with a cylindrical side wall, and the top surface and the bottom surface are respectively connected with the side wall in a sealing way, so that a sealed cavity is formed inside the tank body 11.

FIG. 1 is a schematic view showing the structure of a culture tank. The inside of the tank body 11 is used for containing culture solution, the top part, namely the center of the top surface, of the tank body 11 is provided with a tank nozzle 12, the tank nozzle 12 is communicated with the inside and the outside of the tank body 11, the cross section of the inner surface of the top part of the tank body 1 is gradually reduced from the side wall to the tank nozzle 12, and is gradually increased from the side wall to the joint of the tank nozzle 12; preferably, the top surface is configured as a conical surface, and the spout 12 is configured as the vertex of the cone, however, the invention is not limited to the conical shape, and other structures tapering from bottom to top are also possible, and the specific forms are not described herein, and all of them should be included in the protection scope of the invention.

The piston body 13 is arranged in the tank body 11, the side wall of the piston body 13 is in sealing contact with the side wall of the inner surface of the tank body 11, so that the culture solution is positioned in a cavity between the upper surface of the piston body 13 and the tank body 11, the piston body 13 can move horizontally and ascend and descend along the inner wall of the tank body 11, and the culture solution is always pushed by the piston body 13 to move vertically due to the sealing contact between the piston body 13 and the inner wall of the tank body 11. The upper surface of the piston body 13 has the same shape and size as the top of the inner surface of the can body 11, and when the piston body 13 is lifted to the topmost part, the piston body 13 is in close contact with the top surface of the inner surface of the can body 11, thereby completely extruding the culture solution.

Preferably, the piston body 13 of the present invention is lifted by air pressure, the lower part of the piston body 13 is communicated with the outside air, when the piston body 13 is pressed onto the nozzle 23, the piston body 13 moves downwards to realize the liquid filling, and when the negative pressure is pumped onto the nozzle 23, the piston body 13 moves upwards to realize the liquid pumping. Of course, the piston body 13 can also be driven in other ways, for example, by a lifting structure arranged at the bottom.

The rotary connecting piece 15 is fixed at the bottom of the tank body 11 and is used for being connected with a rotary power device so as to drive the tank body 11 to rotate, the rotary power device can drive the tank body 11 to rotate when rotating, the culture solution can also rotate along with the rotary power device, when the specific rotating speed is reached, substances such as cells in the culture solution are layered due to different masses, and the substances with large masses are positioned on the outer layer.

When separation is needed, the tank body 11 is rotated, cells are layered after reaching the corresponding rotating speed, the piston body 13 is controlled to move upwards at the moment, the culture solution is pushed to be discharged from the tank nozzle 12 above, the top surface of the tank body 11 is conical, so that the liquid in the innermost layer is discharged firstly, the liquid in the outer layer is remained, the discharged part is waste liquid which basically does not contain the cells, the remained liquid contains most of the cells, new culture solution is added into the tank body 11 for continuous culture, and the culture process is kept in a proper condition for cell growth.

According to the cell amplification device, only one tank nozzle 12 is arranged for discharging liquid, the cells are classified by the layering effect generated by centrifugal force, the cells and waste liquid are discharged from the tank nozzle, and the culture solution and the cells are taken out more efficiently; the cells are always in a sealed environment, so that the cells are prevented from being polluted; in addition, the cells are selected by adopting a centrifugal layering mode, and relatively pure target cells can be obtained.

In addition to the above-described embodiments, the cell expansion apparatus of the present invention further includes a lid 2, and the lid 2 is fixedly installed and remains stationary when the tank 11 rotates, in order to be connected to an external pipe. The lid 2 is provided with a nozzle 23 to which an external pipe can be connected, the inner wall of the lid 2 is hermetically connected to the spout 12, and the culture solution discharged from the spout 12 can be discharged from the nozzle 23 of the lid 2. The tank cover 2 and the tank nozzle 12 are rotationally connected through a bearing 21, the outer ring of the bearing 21 is clamped and fixed in the inner cavity of the tank cover 2, the inner ring of the bearing 21 is clamped and fixed outside the tank nozzle 12, the outer surface of the tank nozzle 12 is partially contacted with the inner wall of the tank cover 2, and the contact part keeps sealed. During operation, the tank cover 2 is kept still all the time, only the tank body 11 is rotated, and cell layering in the tank can be realized.

Specifically, the can mouth 12 of the present invention is a stepped cylindrical structure, which can be fitted with the stepped structure of the inner surface of the can lid 2; the sealing gasket 22 is fitted on the spout 12 to maintain a seal with the lid 2, and a multi-layer sealing gasket may be provided to prevent the culture solution from flowing out through the gap between the two.

The design scheme of the tank cover and the tank nozzle ensures that the cell amplification components are relatively independent, can be conveniently connected into any existing automatic cell culture equipment, and does not need to change the structures of other devices and a liquid conveying pipe connected with the culture tank and the like; and equipment maintenance and fault diagnosis are facilitated.

In order to keep a good seal between the can mouth 12 and the can cover 2, at least two sealing gaskets 22 are arranged, the sealing gasket 22 is provided with conical and tilted fins 221, the fins 221 are of a conical structure protruding out of the body of the sealing gasket 22, the fins 221 have elasticity, the fins 221 on the two sealing gaskets 22 are arranged oppositely, an annular groove is arranged on the inner wall of the can cover 2 to install the sealing gasket 22, the groove forms a step surface, the interior of the can cover 2 is stepped, the other sealing gasket 22 is pressed on the step, namely the fins 221 of the two sealing gaskets 22 are arranged oppositely and respectively pressed on the two step surfaces. The static and dynamic sealing under the working conditions of normal pressure, overpressure and negative pressure can be realized by adopting the sealing gaskets 22 with the fins 221 at the upper part and the lower part.

A plurality of mounting grooves 131 are formed in the circumferential direction of the piston body 13 for mounting a packing ring, which is in sealing contact with the inner surface of the can body 11. For convenience of manufacture, the spout 12 is integrally formed with the top surface of the can body 11, and as shown in fig. 2, the periphery of the top surface is fixedly connected with the side wall of the can body 11 through a locking ring 16 by screw threads, which is an assembly explosion diagram between the spout 12 and the can lid 2. The locking ring 16 is of an annular structure, the cross section of the locking ring is L-shaped, threads are arranged on the vertical side wall, and the horizontal section can tightly press the edge of the top surface on the side wall of the tank body 11, so that sealing is realized.

On the basis of any one of the technical schemes and the mutual combination thereof, the invention also comprises a centrifugal tank 3 for sleeving and fixing the culture tank 1, and as shown in figure 3, the centrifugal tank is a structural schematic diagram; the centrifugal tank 3 is positioned outside the culture tank 1 and can drive the culture tank 1 to rotate. The centrifugal tank 3 includes a tank body 31, an outer radiator 32, an inner radiator 33, a semiconductor refrigeration module, a temperature sensor 34, a centrifugal motor 35, and the like.

The tank body 31 is cylindrical and is sleeved outside the culture tank 1 and fixedly connected with the tank cover 2; the outer radiator 32 is arranged on the outer surface of the tank body 31 and is provided with a plurality of groups of radiating fins, and the outer radiator 32 is made of a material with good heat conducting property, so that the contact area with air is increased, and the heat conduction is accelerated; the inner radiator 33 is disposed on the inner surface of the tank body 31 in the gap between the tank body 31 and the culture tank 1, and has a plurality of sets of fins, similar to the structure of the outer radiator 32, the inner radiator 33 and the outer radiator 32 are disposed at opposite positions of the tank body 31, the inner radiator 33 absorbs or diffuses heat at an accelerated rate, so that the culture tank 1 is cooled or heated at a fast rate, and the inner radiator 33 and the outer radiator 32 are close to each other to accelerate heat conduction.

The semiconductor refrigeration module is arranged between the outer radiator 32 and the inner radiator 33; the semiconductor refrigeration module is a flaky electronic component and is provided with a cold surface and a hot surface, when a forward voltage is applied, the hot surface generates heat, and the cold surface refrigerates; when reverse voltage is applied to the component, the cold surface generates heat, and the hot surface refrigerates. The tank body 31 is provided with two inner radiators 33 and two outer radiators 32, four of which are provided, one outer radiator 32 and one inner radiator are paired, and a semiconductor refrigeration module (not shown) is sandwiched between each pair of radiators, and the heat surface of the semiconductor refrigeration module faces the tank.

The temperature sensor 34 is provided on the tank body 31 for detecting the temperature of the liquid inside the culture tank 1, and an infrared temperature sensor may be used. The centrifugal motor 35 is arranged at the bottom of the tank body 31 and can drive the tank body 11 to rotate. During operation, the placing connecting piece 15 of the culture tank 1 is connected with a chuck of a centrifugal motor 35 on the centrifugal tank 3, the tank cover 2 is fixedly connected with the centrifugal tank 3, and the connecting nozzle 23 is connected with a transfusion tube. When the centrifugal motor 35 rotates, the tank body 11, the tank nozzle 12, the inner ring of the bearing 21, the sealing gasket 22 and the like of the culture tank 1 are driven to rotate together, and the tank cover 2 and the tank body 31 are kept fixed, so that the tank cover 2 does not rotate, and the infusion tube connected to the nozzle 23 of the tank cover 2 does not rotate, and thus, the rotation of the tank body 11 and the static state of the tank cover 2 and the infusion tube are realized. Meanwhile, because the fins 221 of the upper sealing gasket and the lower sealing gasket are tightly attached to the small step of the tank cover 2, when liquid is filled into the tank body 11, the pressure in the infusion tube is greater than the external air pressure, and the downward fin 221 of the upper sealing gasket is tightly attached to the step of the tank cover 2, so that the super-air pressure sealing is realized; when liquid is pumped outwards, the pressure in the infusion tube is smaller than the external air pressure, and the fins 221 arranged upwards on the lower sealing gasket are tightly attached to the steps of the tank cover 2, so that negative air pressure sealing is realized.

The air-conditioning tank also comprises an air inlet 36 and an air outlet 37 which are used for communicating the inside and the outside of the tank body 31, and air flow enters the inside of the tank body 31 from the air inlet 36 and is discharged through the air outlet 37. The air inlet 36 and the air outlet 37 are respectively provided with a butt joint structure for connecting an air inlet pipe and an air outlet pipe.

The positions and the number of the air inlets 36 and the air outlets 37 can be adjusted correspondingly according to the use requirements, preferably, two air inlets 36 are vertically arranged, one air outlet 37 is arranged, the air outlet 37 is arranged between the two air inlets 36, and the two air inlets 36 and the air outlet 37 are vertically arranged.

The tank body 31 is provided with an air guide groove, as shown in fig. 4A, the air guide groove is a specific structure diagram of the air guide groove, the air guide groove is a hollow interlayer arranged on the tank body 31, the air inlet 36 and the air outlet 37 are connected to the air guide groove, a baffle 38 is arranged in the air guide groove and used for separating the space between the tank body 31 and the tank body 11 at intervals, as shown in fig. 4B, the baffle 38 is a perspective structure diagram of the air guide groove, the baffle 38 is of a plurality of bent S-shaped structures and used for isolating the air inlet 36 and the air outlet 37 from each other, air enters a gap between the tank body 31 and the tank body 11 from the air inlet 36, and due to the separation of the baffle 38, the air can only be discharged from the. The air that gets into circulates a week and discharges, and the heat transfer effect is more abundant.

The inner side of the air guide groove divides three structures of two air inlets 36 and one air outlet 37 into two parts, wherein the air inlet 36 faces to the right, and the air outlet 37 faces to the left. When the air conditioner works, the air inlet 36 is externally connected with a sealed pneumatic source, so that air always enters from the air inlet 36 and exits from the air outlet 37, the air conditioner has heat insulation and sealing functions, and heat exchange with external air is avoided. When the infrared temperature sensor detects that the temperature of the liquid in the culture tank 1 is lower than the target temperature, positive voltage is applied to the refrigeration module to enable the inner radiator to generate heat, and the temperature in the whole tank is kept uniform through air flow; when the infrared temperature sensor detects that the temperature of the liquid in the culture tank 1 is higher than the target temperature, reverse voltage is applied to the refrigeration module to refrigerate the inner radiator, so that the temperature of the liquid in the culture tank 1 is kept as the target temperature, and the purposes of constant temperature and temperature equalization are achieved.

The invention also comprises a movable cover 39 arranged at the top of the can body 31, and a discharge hole 391 for fixing the can cover 2 is arranged in the middle of the movable cover 39. The movable cover 39 can be opened movably to facilitate taking and putting the culture tank 1. The tank cover 2 is inserted into the discharge hole 391 and fixed by the discharge hole 391, providing a stable supporting force to the circumferential direction of the tank cover 2.

Specifically, the movable cover 39 includes two semicircular covers spliced to form a complete cover body, each semicircular cover is provided with a semicircular opening, so that a discharge hole 391 can be formed; the turning column 392 is disposed at the corner of the edge of one side of the two semicircular covers close to each other, so that the two semicircular covers can be opened by turning horizontally, and of course, the specific structure and opening mode of the movable cover 39 of the invention can be adjusted and improved correspondingly, as long as the cover 2 can be fixed to discharge material, and the improvements are all included in the protection scope of the invention. The catch 393 is arranged at a position opposite to the rotating column 392 and used for locking and fixing the two semicircular covers, and the cover 2 can be clamped after the two semicircular covers are locked.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell expansion apparatus comprising a culture tank (1), the culture tank (1) comprising:

the culture medium tank comprises a tank body (11) for containing culture medium, wherein a tank nozzle (12) is arranged in the center of the top of the tank body (11), the cross section of the inner surface of the top of the tank body (11) is gradually reduced from the side wall to the tank nozzle (12), and is gradually increased from the side wall to the joint of the tank nozzle (12);

the side wall of the piston body (13) is in sealing contact with the side wall of the inner surface of the tank body (11), and the piston body (13) can be horizontally moved and lifted along the inner wall of the tank body (11); the upper surface of the piston body (13) can be in contact with the top surface of the inner surface of the tank body (11);

and the rotating connecting piece (15) is fixed at the bottom of the tank body (11) and is used for connecting a rotating power device so as to drive the tank body (11) to rotate.

2. The cell expansion apparatus according to claim 1, further comprising a lid (2) fixedly disposed, wherein the lid (2) is provided with a nozzle (23) capable of connecting with an external pipeline; the inner wall of the tank cover (2) is connected with the tank nozzle (12) in a sealing mode, and the tank cover (2) is rotatably connected with the tank nozzle (12) through a bearing (21).

3. The cell expansion apparatus according to claim 2, wherein the mouth (12) has a stepped cylindrical structure capable of fitting with the stepped structure of the inner surface of the lid (2); the can mouth (12) is sleeved with a sealing gasket (22) to keep sealing with the can cover (2).

4. The cell amplification device according to claim 3, wherein the number of the sealing gaskets (22) is at least two, and the sealing gaskets (22) are provided with conical tilted fins (221); the fins (221) on the two sealing gaskets (22) are oppositely arranged and are pressed on the stepped surface of the tank cover (2).

5. The cell expansion apparatus according to claim 4, wherein a plurality of mounting grooves (131) are formed in the circumferential direction of the piston body (13) for mounting a sealing ring in sealing contact with the inner surface of the can (11); the tank nozzle (12) and the top surface of the tank body (11) are integrally arranged and are fixedly connected with the inner wall of the tank body (11) through a locking ring (16) through threads.

6. The cell expansion apparatus according to any one of claims 2 to 5, further comprising a centrifuge tank (3) for collectively fixing the culture tank (1), wherein the centrifuge tank (3) comprises:

the tank body (31) is sleeved on the outer side of the culture tank (1) and fixedly connected with the tank cover (2);

the outer radiator (32) is arranged on the outer surface of the tank body (31) and is provided with a plurality of groups of radiating fins;

an inner radiator (33) which is arranged on the inner surface of the tank body (31), is provided with a plurality of groups of radiating fins, and is arranged at a position opposite to the outer radiator (32) on the tank body (31);

a semiconductor refrigeration module disposed between the outer radiator (32) and the inner radiator (33);

a temperature sensor (34) for detecting the temperature of the liquid inside the culture tank (1);

the centrifugal motor (35) is arranged at the bottom of the tank body (31) and can drive the tank body (11) to rotate.

7. The cell amplification apparatus according to claim 6, further comprising an air inlet (36) and an air outlet (37) for communicating the inside and the outside of the can body (31), wherein the air flow enters the inside of the can body (31) from the air inlet (36) and is discharged through the air outlet (37).

8. The cell expansion apparatus according to claim 7, wherein the air inlets (36) are vertically arranged in two, the air outlet (37) is arranged in one, and the air outlet (37) is arranged between the two air inlets (36); the air guide tank is characterized in that an air guide groove is formed in the tank body (31), the air inlet (36) and the air outlet (37) are connected to the air guide groove, a baffle (38) is arranged in the air guide groove and used for isolating the air inlet (36) and the air outlet (37) from each other, and air enters from the air inlet (36) and surrounds the tank body (31) for a circle and is discharged from the air outlet (37).

9. The cell expansion apparatus according to claim 8, further comprising a movable lid (39) provided on the top of the can body (31), wherein a discharge hole (391) for fixing the can lid (2) is formed in the middle of the movable lid (39).

10. The cell amplification device according to claim 9, wherein the movable cover (39) comprises two semicircular covers which are spliced to form a complete cover body, and a rotating column (392) is arranged at one side edge of the two semicircular covers which are close to each other, so that the two semicircular covers can be horizontally rotated and opened; and a lock catch (393) is arranged at the position opposite to the rotating column (392) and used for locking and fixing the two semicircular covers.