CN207537459U - A kind of cell expansion apparatus - Google Patents

Abstract

The utility model discloses a kind of cell expansion apparatus, and including culture tank, tank mouth is set in the center of top of tank body；The cross section of tank body top inner surface is gradually reduced from side-walls to tank mouth, and is gradually risen from side wall to the junction of tank mouth；The side wall of piston body and the inner surface side wall of tank body are in sealing contact, and piston body can make translation lifting along the inner wall of tank body, and culture solution can discharge by when rising at tank mouth, and piston body upper surface can be contacted with the fitting of the top surface of the inner surface of tank body.Rotary connector is fixed on the bottom of tank body, for connecting rotary power unit, tank body to be driven to rotate.Tank body is driven to rotate by rotary connector, the cell in culture solution generates be layered under the action of the centrifugal force, the cell category in different layers is different because of quality difference；Since the top surface of tank body is gradually reduced from bottom to top, during promotion, internal layer liquid is first discharged, and is discharged after outer layer liquid, and relatively pure cell or other liquid are obtained by layering.

Description

A kind of cell expansion apparatus

Technical field

The utility model is related to cell preparation techniques fields, further relate to a kind of cell expansion apparatus.

Background technology

Patient needs to remove tumour cell over the course for the treatment of, and kill tumour cell is not selective, while can also kill Dead a large amount of healthy cell, because individual physique level is not quite similar, some in patient body healthy cell reproduction speed not The reproduction speed of tumour cell is fast, and final the result is that while tumour cell elimination finishes, useful healthy cell is also killed Light, patient finally can still face death.It is above-mentioned to solve the problems, such as, there is a kind of novel tumor therapeuticing method： Cell therapy, its main thought are exactly to extract healthy cell sample from the patient, by external device culture, are copied big Then patient's cell of amount is input in patient body, patient is helped to get well again.

Cell culture is a kind of method of research zooblast behavior founded earlier 1900s, is taken out from in-vivo tissue thin Born of the same parents, growing environment in analogue body are bred cell growth under proper temperature, acid-base value, a kind of sterile and nutritional condition, are tieed up Hold its structure and function.Cell preparation is cell end product that high-purity is produced with cell culture protocol, for scientific research with The process of clinical field.

The culture and preparation of cell need to meet appropriate external condition, in the cell augmentation apparatus generally used at present, Culture tank is common bottle, after shape leads to centrifugation, hierarchical operations, needs first to extract useful cell, is then fallen again useless Useful cell is finally refunded culture tank by liquid again, and cell passes in and out back and forth, is easily damaged, and inefficiency.

Utility model content

The utility model provides a kind of cell expansion apparatus, it is not necessary to and waste liquid is poured out, keeps cell pure, it is efficient and will not Damaging cells, concrete scheme are as follows：

A kind of cell expansion apparatus, including culture tank, the culture tank includes：

Tank body, for containing culture solution, center of top sets tank mouth, the cross section of the tank body top inner surface for inside It is gradually reduced from side-walls to the tank mouth, and is gradually risen from side wall to the junction of the tank mouth；

Piston body, the inner surface side wall of side wall and the tank body is in sealing contact, and the piston body can be along the tank body Inner wall make translation lifting；The piston body upper surface can be bonded with the top surface of the tank inner surface and contact；

Rotary connector is fixed on the bottom of the tank body, for connecting rotary power unit, the tank body to be driven to turn It is dynamic.

Optionally, the cover of fixed setting is further included, what setting can connect exterior line on the cover connects mouth；It is described The inner wall of cover is tightly connected with the tank mouth, and the cover keeps being rotatablely connected with the tank mouth by bearing.

Optionally, the tank mouth is step-like column structure, can be with the step structure of the inner surface of the cover Cooperation assembling；Gasket is set on the tank mouth to keep sealing with the cover.

Optionally, the gasket at least sets two, and the fin of tapered tilting is set on the gasket；Two The fin on the gasket is oppositely arranged, and is crimped on the cascaded surface of the cover.

Optionally, the week of the piston body sets up multiple mounting grooves, is sealed for installing with the tank inner surface The sealing ring of contact；The top surface integrated setting of the tank mouth and the tank body, and by clamp ring and the tank body Wall is threadably secured connection.

Optionally, the tophan box for being set with the fixed culture tank is further included, the tophan box includes：

Can body is set in the outside of the culture tank, is fixedly connected with the cover；

Outer radiator is set to the outer surface of the can body, sets multigroup cooling fin；

Inner radiator is set to the inner surface of the can body, sets multigroup cooling fin, and be set to the outer radiator The relative position of the can body；

Semiconductor refrigerating module is set between the outer radiator and the inner radiator；

Temperature sensor, for detecting the fluid temperature inside the culture tank；

Motor is centrifuged, is set to the bottom of the can body, can drive and be rotated with the tank body.

Optionally, further include to connect the air inlet and air outlet inside and outside the can body, air-flow from the air inlet into Enter inside the can body, and discharged through the air outlet.

Optionally, the air inlet is vertically arranged two, and air outlet sets one, and the air outlet is set to described in two Between air inlet；Wind guide tank is set on the can body, and the air inlet and the air outlet are connected on the wind guide tank, described Baffle is set in wind guide tank, and for the air inlet and the air outlet is mutually isolated, wind enters from the air inlet to be surround The can body is discharged for one week from the air outlet.

Optionally, the removable cover being set at the top of the can body is further included, the centre of the removable cover opens up to fix The relief hole of the cover.

Optionally, the removable cover includes two and half domes for being spliced to form complete cover, and two and half domes are close to each other One side edge setting Rotational Cylindrical, two and half domes is enable to horizontally rotate opening；The position setting opposite with the Rotational Cylindrical Lock, for two pieces of semicircle cover locks to be fastened.

The utility model discloses a kind of cell expansion apparatus, and including culture tank, culture tank specifically includes tank body, piston The structures such as body, rotary connector, the inside of tank body set tank mouth for containing culture solution, in the center of top of tank body, for Outer output culture solution；The cross section of tank body top inner surface is gradually reduced from side-walls to tank mouth, and from side wall to the company of tank mouth The place of connecing gradually rises, and when culture solution moves upwards under the promotion of piston body, culture solution is made finally to collect at tank mouth and is discharged；It is living The side wall of cock body and the inner surface side wall of tank body are in sealing contact, and piston body can make translation lifting along the inner wall of tank body, during rising Culture solution can be discharged at tank mouth, piston body upper surface can be bonded with the top surface of the inner surface of tank body and contact, so as to Culture solution is discharged completely.Rotary connector is fixed on the bottom of tank body, for connecting rotary power unit, tank body to be driven to turn It is dynamic.

When needing to detach cell, tank body is driven to rotate by rotary connector, the cell in culture solution because of quality difference, It generates and is layered under the action of the centrifugal force, the cell category in different layers is different；Control piston body rises at this time, and culture solution is upward It pushes, since the top surface of tank body is gradually reduced from bottom to top, during promotion, innermost layer liquid is discharged at first, more outer The more late discharge of liquid of layer, so as to obtain relatively pure cell or other liquid.

The cell expansion apparatus of the utility model only sets a tank mouth for drain, therefore generated by centrifugal force Layered effect realizes the classification of cell, and cell and waste liquid are discharged from tank mouth, makes in environment of the cell always in sealing, avoids Cell is contaminated.

Description of the drawings

It in order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor Under, it can also be obtained according to these attached drawings other attached drawings.

Fig. 1 is the structure diagram of culture tank；

Assembling explosive views of the Fig. 2 between tank mouth and cover；

Fig. 3 is the structure diagram of tophan box；

Fig. 4 A are a kind of concrete structure diagram of wind guide tank；

Fig. 4 B are the perspective structure figure of wind guide tank.

Including：

Culture tank 1, tank body 11, tank mouth 12, piston body 13, mounting groove 131, clamp ring 14, rotary connector 15, cover 2, Bearing 21, fin 221, connects mouth 23, tophan box 3, can body 31, outer radiator 32, inner radiator 33, temperature sensing at gasket 22 Device 34, centrifugation motor 35, air inlet 36, air outlet 37, baffle 38, removable cover 39, relief hole 391, Rotational Cylindrical 392, lock 393。

Specific embodiment

The core of the utility model is to provide a kind of cell expansion apparatus, it is not necessary to pour out waste liquid, keep cell pure, effect Rate is high and will not damaging cells.

In order to which those skilled in the art is made to more fully understand the technical solution of the utility model, below in conjunction with attached drawing and Explanation is described in detail to the cell expansion apparatus of the utility model in specific embodiment.

Cell expansion apparatus provided by the utility model includes culture tank 1, and culture tank 1 further comprises tank body 11, tank The structures such as mouth 12, piston body 13, rotary connector 15, tank body 11 are equipped with columnar side wall, top surface and bottom surface respectively with side wall It is tightly connected, the inside of tank body 11 is made to form the cavity of sealing.

As shown in Figure 1, the structure diagram for culture tank.The inside of tank body 11 for containing culture solution, top namely The center setting tank mouth 12 of top surface, tank mouth 12 connect the inside and outside of tank body 11, the cross section of 1 top inner surface of tank body from side-walls to Tank mouth 12 is gradually reduced, and is gradually risen from side wall to the junction of tank mouth 12；Preferably, top surface should be set as circular cone Face, tank mouth 12 are arranged on the vertex of taper, certainly, the utility model be also not limited to it is coniform, can also be other from it is lower to Upper tapered structure, no longer illustrates one by one herein, these specific forms should all be included in the protection model of the utility model Within enclosing.

Piston body 13 is arranged on the inside of tank body 11, and the inner surface side wall of side wall and tank body 11 is in sealing contact, makes culture Liquid is in the cavity between 13 upper surface of piston body and tank body 11, and piston body 13 can make translation along the inner wall of tank body 11 and rise Drop, since piston body 13 is contacted with 11 inner wall sealing of tank body, culture solution is pushed by piston body 13 move up and down always.It is living The upper surface of cock body 13 is identical with the top shape and size of 11 inner surface of tank body, living when piston body 13 rises to top Cock body 13 is contacted with the top surface fitting of 11 inner surface of tank body, so as to which culture solution be squeezed out completely.

Preferably, the piston body 13 in the utility model is realized by air pressure and lifted, and the lower section and the external world of piston body 13 are empty Gas phase is led to, when toward connect pressurize on mouth 23 when, piston body 13 moves down, and realizes that liquid pours into, when toward when connecing on mouth 23 pumping negative pressure, piston Body 13 moves up, and realizes liquid extraction.Certainly, other type of drive, such as bottom setting lifting knot can also be used in piston body 13 Structure.

Rotary connector 15 is fixed on the bottom of tank body 11, for connecting rotary power unit, tank body 11 to be driven to rotate, Rotary power unit can drive tank body 11 to rotate when rotating, and culture solution can also rotate therewith, and when reaching specific rotating speed, cultivate The substances such as the cell in liquid because quality difference is layered, quality it is big be located at outer layer.

When detachment is desired, rotate tank body 11, cell is layered after reaching corresponding rotating speed, controls piston body 13 at this time It moves upwards, culture solution is pushed to be discharged from the tank mouth 12 of top, because the top surface of tank body 11 is coniform, therefore in innermost layer Liquid can first be discharged, leave outer layer liquid, discharge part is to contain substantially no the waste liquid of cell, and the liquid left includes big Part cell adds in new culture solution to tank body 11 and continues to cultivate, and incubation is maintained at the suitable condition of cell growth.

The cell expansion apparatus of the utility model only sets a tank mouth 12 for drain, point generated by centrifugal force Layer effect realizes the classification of cell, and cell and waste liquid are discharged from tank mouth, the extraction efficiency higher of culture solution and cell；Cell begins In environment eventually in sealing, cell is avoided to be contaminated；In addition, selecting cell in a manner that centrifugation is layered, can obtain Relatively pure target cell.

On the basis of said program, in order to more preferably be connect with exterior line, the cell expansion apparatus of the utility model is also Including cover 2, cover 2 is fixed setting, is remained stationary as when tank body 11 rotates.Setting can connect exterior line on cover 2 Connect mouth 23, inner wall and the tank mouth 12 of cover 2 are tightly connected, and the culture solution discharged from tank mouth 12 can connect mouth from cover 2 It is discharged in 23.Cover 2 keeps being rotatablely connected with tank mouth 12 by bearing 21, and the outer ring of bearing 21 is fixed on the interior of cover 2 In chamber, the inner ring of bearing 21 is fixed on except tank mouth 12, is partly connect between the outer surface of tank mouth 12 and the inner wall of cover 2 It touches, contact portion keeps sealing.Cover 2 remains static during work, only rotates tank body 11, it can be achieved that tank inner cell point Layer.

Specifically, tank mouth 12 is step-like column structure in the utility model, can be with the ladder of the inner surface of cover 2 Shape respective outer side edges are assembled；It is set with gasket 22 on tank mouth 12 to keep sealing with cover 2, multilayer gasket, protection culture can be set Liquid can not be flowed out from gap between the two.

The designing scheme of cover and tank mouth so that cell expansion module is relatively independent in the utility model, can easily connect Enter any automatic culture device of existing cell, do not need to change other apparatus structures and the woven hose being connected with culture tank etc.； Facilitate plant maintenance and fault diagnosis.

In order to make to keep good sealing between tank mouth 12 and cover 2, gasket 22 at least sets two, and gasket 22 The fin 221 of the upper tapered tilting of setting, fin 221 is the cone-shaped structure for protruding from 22 ontology of gasket, and fin 221 has Elasticity, the fin 221 on two gaskets 22 are oppositely arranged, and the groove installation gasket of annular is set on the inner wall of cover 2 22, this groove forms a step surface, and the inside of cover 2 is stepped in itself, another gasket 22 be crimped on ladder namely The fin 221 of two gaskets 22 is oppositely arranged, and is crimped on respectively on two cascaded surfaces.Using close with fin 221 up and down Packing 22 can realize normal pressure, superpressure, the static state under negative pressure operating mode, dynamic sealing.

Multiple mounting grooves 131 are set up the week of piston body 13, for installing sealing ring, sealing ring and 11 inner surface of tank body It is in sealing contact.In order to facilitate processing and manufacturing, the top surface integrated setting of tank mouth 12 and tank body 11, as shown in Fig. 2, for tank mouth 12 with Assembling explosive view between cover 2, the surrounding of top surface is threadably secured by clamp ring 16 with the side wall of tank body 11 to be connect.Lock Collar 16 is cyclic structure, and cross section is L-shaped, is threaded in upright side walls, and top edge can be then pressed on by horizontal segment On the side wall of tank body 11, so as to fulfill sealing.

In any of the above-described technical solution and its on the basis of being combined with each other, the utility model further includes to be set with fixed training The tophan box 3 of tank 1 is supported, as shown in figure 3, the structure diagram for tophan box；Tophan box 3 is located at except culture tank 1, can drive training Tank 1 is supported to rotate.Tophan box 3 includes can body 31, outer radiator 32, inner radiator 33, semiconductor refrigerating module, temperature and passes The structures such as sensor 34, centrifugation motor 35.

Can body 31 is tubular, is set in the outside of culture tank 1, is fixedly connected with cover 2；Outer radiator 32 is set to can body 31 Outer surface, multigroup cooling fin is set, and outer radiator 32 is made of the good material of heat conductivility, increases and the contact surface of air Product accelerates heat conduction；Inner radiator 33 is set to the inner surface of can body 31, in the gap between can body 31 and culture tank 1, if Multigroup cooling fin is put, similar to the structure of outer radiator 32, inner radiator 33 is set to the opposite of can body 31 with outer radiator 32 Position, inner radiator 33 accelerates to absorb or diffusion heat, makes 1 fast cooling of culture tank or heating, inner radiator 33 and outer heat dissipation Device 32 is close to each other, with the conduction of accelerated heat.

Semiconductor refrigerating module is set between outer radiator 32 and inner radiator 33；Semiconductor refrigerating module is a kind of The electronic component of shape has huyashi-chuuka (cold chinese-style noodles) and hot face Liang Ge faces, when applying forward voltage, hot face fever, and huyashi-chuuka (cold chinese-style noodles) refrigeration；When to this When component applies backward voltage, huyashi-chuuka (cold chinese-style noodles) fever, hot face refrigeration.Two panels inner radiator 33 is installed respectively, outside two panels on can body 31 Radiator 32, totally four, a piece of outer radiator 32 is a pair with a piece of inner radiator, and each pair of radiator is sandwiched between semiconductor system Chill block (not shown), the heat of semiconductor refrigerating module are faced in tank.

Temperature sensor 34 is arranged on can body 31, and for detecting the fluid temperature inside culture tank 1, infrared temperature can be used Spend inductor.Centrifugation motor 35 is set to the bottom of can body 31, and tank body 11 can be driven to rotate.During work, the placement of culture tank 1 Connector 15 is connected with centrifuging the chuck of motor 35 on tophan box 3, and cover 2 is fixedly connected with tophan box 3, is connect mouth 23 and is connect infusion Pipe.Turn together when centrifuging the tank body 11 that culture tank 1 is driven during the rotation of motor 35, tank mouth 12, the inner ring of bearing 21, gasket 22 etc. Dynamic, since cover 2 and can body 31 remain fixed, cover 2 does not rotate, and it is also not to be connected on the woven hose that cover 2 is connect on mouth 23 Rotation, it is rotated in this way, just realizing tank body 11, cover 2 and woven hose are static.Simultaneously as the wing of upper and lower two gaskets Piece 221 is tightly attached on the small stair of cover 2, and when the liquid filling body in tank body 11, the pressure in woven hose is more than external air Pressure, the fin 221 that upper gasket is set downward can be close to the step of cover 2, realize excess air pressure sealing；When drawing liquid body outward When, the interior pressure of woven hose is less than external air pressure, and the fin 221 that lower seal pad is set upward is just close to the platform of cover 2 Rank realizes means of press seals of doing something in a fit of pique.

It further includes for connecting the air inlet 36 and air outlet 37 inside and outside can body 31, air-flow enters can body 31 from air inlet 36 Inside, and discharged through air outlet 37.Air inlet 36 and air outlet 37 set docking structure respectively, for connecting blast pipe and outlet air Pipe.

The position and quantity of air inlet 36 and air outlet 37 can be adjusted correspondingly according to requirements, it is preferable that this Air inlet 36 is vertically arranged setting two in utility model, and air outlet 37 sets one, and air outlet 37 is set to two air inlets Between 36, two air inlets 36 and an air outlet 37 are vertically arranged setting.

Wind guide tank on can body 31 is set, is a kind of concrete structure diagram of wind guide tank, wind guide tank is is set to as shown in Figure 4 A Hollow sandwich on can body 31, air inlet 36 and air outlet 37 are connected on wind guide tank, and baffle 38 is set in wind guide tank, for inciting somebody to action Space interval separates between can body 31 and tank body 11, is the perspective structure figure of wind guide tank, baffle 38 is in multiple curved as shown in Figure 4 B The S type structures of folding, for air inlet 36 and air outlet 37 is mutually isolated, wind from air inlet 36 enter can body 31 and tank body 11 it Between gap in, due to the separation of baffle 38, wind can only discharge for 31 1 weeks around can body from air outlet 37.The air circulation of entrance It discharges within one week, heat transfer effect is more abundant.

By two air inlets 36 and an air outlet 37, totally three structures are divided into two parts for the inside of wind guide tank, air inlet Mouthfuls 36 towards the right side, and air outlet 37 is towards a left side.During work, the external closed pneumatic source of air inlet 36, guarantee always have gas from air inlet 36 into, Go out from air outlet 37, there is heat-insulated and sealing function, ensure not exchange with ambient atmos hair body heat.When infrared temperature sensor is examined When measuring the fluid temperature in culture tank 1 less than target temperature, just apply forward voltage in refrigeration module, inner radiator is allowed to send out Heat, and keep temperature in entire tank uniform by air flowing；The liquid temperature in culture tank 1 is detected when infrared temperature sensor When degree is higher than target temperature, just applies backward voltage in refrigeration module, inner radiator is allowed to freeze, to keep liquid in culture tank 1 Temperature is target temperature, achievees the purpose that constant temperature, samming.

The utility model further includes the removable cover 39 for being set to 31 top of can body, and the centre of removable cover 39 opens up to fix The relief hole 391 of cover 2.Removable cover 39 movable can be opened, and culture tank 1 is picked and placeed to facilitate.Cover 2 is inserted into relief hole 391, And fixation is realized by relief hole 391, provide stable support force to the circumferential of cover 2.

Specifically, removable cover 39 includes two and half domes for being spliced to form complete cover, and each semicircle covers to be set respectively Semicircular trepanning can form relief hole 391；Two and half domes one side edge corner setting Rotational Cylindrical close to each other 392, two and half domes is enable to horizontally rotate opening, certainly, the concrete structure and opening of removable cover 39 in the utility model Mode can make corresponding adjustment and improve, as long as can play fixed cover 2 realizes discharge, these improvement should all wrap Containing being within the protection scope of the utility model.In opposite with Rotational Cylindrical 392 position setting lock 393, for by two and half Dome is locked, and is can be realized after two pieces of semicircle cover lockings by 2 clamping of cover.

The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or new using this practicality Type.A variety of modifications of these embodiments will be apparent for those skilled in the art, determine herein The General Principle of justice, can be realized in other embodiments without departing from the spirit or scope of the present utility model.Cause This, the utility model is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein The most wide range consistent with features of novelty.

Claims (10)

1. a kind of cell expansion apparatus, which is characterized in that including culture tank (1), the culture tank (1) includes：

Tank body (11), for containing culture solution, center of top sets tank mouth (12), tank body (11) top inner surface for inside Cross section be gradually reduced from side-walls to the tank mouth (12), and gradually risen from side wall to the junction of the tank mouth (12) It is high；

Piston body (13), the inner surface side wall of side wall and the tank body (11) is in sealing contact, and the piston body (13) being capable of edge The inner wall of the tank body (11) makees translation lifting；It piston body (13) upper surface can be with the top of the tank body (11) inner surface Face paste splice grafting touches；

Rotary connector (15) is fixed on the bottom of the tank body (11), for connecting rotary power unit, to drive the tank Body (11) rotates.

2. cell expansion apparatus according to claim 1, which is characterized in that the cover (2) of fixed setting is further included, it is described What setting can connect exterior line on cover (2) connects mouth (23)；The inner wall of the cover (2) connects with the tank mouth (12) sealing It connects, the cover (2) keeps being rotatablely connected with the tank mouth (12) by bearing (21).

3. cell expansion apparatus according to claim 2, which is characterized in that the tank mouth (12) is step-like column knot Structure can coordinate with the step structure of the inner surface of the cover (2) and assemble；Gasket (22) is set on the tank mouth (12) It keeps sealing with the cover (2).

4. cell expansion apparatus according to claim 3, which is characterized in that the gasket (22) at least sets two, And the fin (221) of tapered tilting is set on the gasket (22)；The fin on two gaskets (22) (221) it is oppositely arranged, is crimped on the cascaded surface of the cover (2).

5. cell expansion apparatus according to claim 4, which is characterized in that setting up in week for the piston body (13) is more A mounting groove (131), for installing and the tank body (11) inner surface sealing ring in sealing contact；The tank mouth (12) with it is described The top surface integrated setting of tank body (11), and company is threadably secured by the inner wall of clamp ring (16) and the tank body (11) It connects.

6. according to claim 2 to 5 any one of them cell expansion apparatus, which is characterized in that further include to be set with fixation The tophan box (3) of the culture tank (1), the tophan box (3) include：

Can body (31) is set in the outside of the culture tank (1), is fixedly connected with the cover (2)；

Outer radiator (32), is set to the outer surface of the can body (31), sets multigroup cooling fin；

Inner radiator (33) is set to the inner surface of the can body (31), sets multigroup cooling fin, and with the outer radiator (32) it is set to the relative position of the can body (31)；

Semiconductor refrigerating module is set between the outer radiator (32) and the inner radiator (33)；

Temperature sensor (34), for detecting the internal fluid temperature of the culture tank (1)；

Motor (35) is centrifuged, is set to the bottom of the can body (31), can drive and be rotated with the tank body (11).

7. cell expansion apparatus according to claim 6, which is characterized in that further include to connect in the can body (31) Outer air inlet (36) and air outlet (37), air-flow enter the can body (31) inside, and described in warp from the air inlet (36) Air outlet (37) is discharged.

8. cell expansion apparatus according to claim 7, which is characterized in that the air inlet (36) is vertically arranged two, Air outlet (37) sets one, and the air outlet (37) is set between two air inlets (36)；On the can body (31) Wind guide tank is set, and the air inlet (36) and the air outlet (37) are connected on the wind guide tank, are set in the wind guide tank Baffle (38), for the air inlet (36) and the air outlet (37) is mutually isolated, wind enters from the air inlet (36) It is discharged around the can body from the air outlet (37) within (31) one weeks.

9. cell expansion apparatus according to claim 8, which is characterized in that further include and be set at the top of the can body (31) Removable cover (39), the centre of the removable cover (39) opens up the relief hole (391) for fixing the cover (2).

10. cell expansion apparatus according to claim 9, which is characterized in that the removable cover (39) is including being spliced to form Two and half domes of complete cover, two and half domes one side edge setting Rotational Cylindrical (392) close to each other, make two and half domes Opening can be horizontally rotated；Opposite with the Rotational Cylindrical (392) position setting lock (393), for by two pieces of semicircle cover locks It fastens.