CN107254467B - 常温保存核酸标本的方法及其产品与使用方法 - Google Patents
常温保存核酸标本的方法及其产品与使用方法 Download PDFInfo
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- CN107254467B CN107254467B CN201710573403.9A CN201710573403A CN107254467B CN 107254467 B CN107254467 B CN 107254467B CN 201710573403 A CN201710573403 A CN 201710573403A CN 107254467 B CN107254467 B CN 107254467B
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- 229910001928 zirconium oxide Inorganic materials 0.000 claims abstract description 11
- 239000012488 sample solution Substances 0.000 claims abstract description 8
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- 238000005406 washing Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 27
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 claims description 24
- 239000006285 cell suspension Substances 0.000 claims description 12
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN108410862A (zh) * | 2018-05-25 | 2018-08-17 | 安康(上海)生物科技有限公司 | 长期基因组dna的保存方法及试剂盒 |
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EP2118302A4 (en) * | 2007-01-16 | 2013-05-15 | Joseph G Utermohlen | PARTICLE MATRIX FOR STORING BIOMOLECULES |
CN103756998A (zh) * | 2014-01-23 | 2014-04-30 | 上海交通大学 | 一种动物核酸样本常温保存试剂及其应用 |
CN103966189A (zh) * | 2014-04-30 | 2014-08-06 | 厦门安普利生物工程有限公司 | 一种提取ung酶的方法 |
KR101777168B1 (ko) * | 2015-12-30 | 2017-09-11 | 주식회사 노블바이오 | 별도의 세포 용해 과정 없이 핵산을 추출하기 위한 검체 수송 배지 조성물 및 이의 용도 |
CN106244582A (zh) * | 2016-08-30 | 2016-12-21 | 苏州凡济医疗器械有限公司 | 一种口腔拭子核酸常温保存方法 |
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Address after: 065201 Fucheng four phase 6-2-1103 room, Sanhe Yanjiao Development Zone, Langfang, Hebei Applicant after: Beijing Langyou science and Technology Co., Ltd. Address before: 100176 Beijing Daxing District economic and Technological Development Zone, 18 South West Road, block C, 3 floor, 306 room. Applicant before: Beijing carbon element technology Co., Ltd. |
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Inventor after: Li Kun Inventor before: Liu Siyuan Inventor before: Li Kun |
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Effective date of registration: 20200820 Address after: 101100 - 044, 1 / F, building 18, 249 Anshun Road, Yongshun Town, Tongzhou District, Beijing Patentee after: Beijing Haimai Technology Co., Ltd Address before: 065201 Fucheng four phase 6-2-1103 room, Sanhe Yanjiao Development Zone, Langfang, Hebei Patentee before: Beijing Langyou science and Technology Co.,Ltd. |
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