CN107236761A - 提高昆虫细胞瞬时转染和稳定表达蛋白表达量的方法 - Google Patents
提高昆虫细胞瞬时转染和稳定表达蛋白表达量的方法 Download PDFInfo
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- CN107236761A CN107236761A CN201710442870.8A CN201710442870A CN107236761A CN 107236761 A CN107236761 A CN 107236761A CN 201710442870 A CN201710442870 A CN 201710442870A CN 107236761 A CN107236761 A CN 107236761A
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- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/70—Invertebrates
- A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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- A01K2267/01—Animal expressing industrially exogenous proteins
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Abstract
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CN201710442870.8A CN107236761B (zh) | 2017-06-13 | 2017-06-13 | 提高昆虫细胞瞬时转染和稳定表达蛋白表达量的方法 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110129365A (zh) * | 2019-05-22 | 2019-08-16 | 北京景达生物科技有限公司 | 一种高效稳定瞬时表达重组蛋白的方法及其应用 |
CN110551754A (zh) * | 2019-07-26 | 2019-12-10 | 佛山汉腾生物科技有限公司 | Cho细胞的瞬时转染方法及重组cho细胞 |
CN113430227A (zh) * | 2020-03-23 | 2021-09-24 | 佛山汉腾生物科技有限公司 | 制备稳定细胞池的方法、蛋白表达方法及试剂盒 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105002132A (zh) * | 2015-08-19 | 2015-10-28 | 中国科学院武汉病毒研究所 | 一种在多种昆虫细胞表达外源蛋白的Ⅱ组甲型杆状病毒Bacmid及应用 |
CN106755093A (zh) * | 2016-11-30 | 2017-05-31 | 中山汉腾生物科技有限公司 | 果蝇细胞瞬时转染的工艺 |
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- 2017-06-13 CN CN201710442870.8A patent/CN107236761B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105002132A (zh) * | 2015-08-19 | 2015-10-28 | 中国科学院武汉病毒研究所 | 一种在多种昆虫细胞表达外源蛋白的Ⅱ组甲型杆状病毒Bacmid及应用 |
CN106755093A (zh) * | 2016-11-30 | 2017-05-31 | 中山汉腾生物科技有限公司 | 果蝇细胞瞬时转染的工艺 |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110129365A (zh) * | 2019-05-22 | 2019-08-16 | 北京景达生物科技有限公司 | 一种高效稳定瞬时表达重组蛋白的方法及其应用 |
CN110129365B (zh) * | 2019-05-22 | 2023-05-05 | 北京景达生物科技有限公司 | 一种高效稳定瞬时表达重组蛋白的方法及其应用 |
CN110551754A (zh) * | 2019-07-26 | 2019-12-10 | 佛山汉腾生物科技有限公司 | Cho细胞的瞬时转染方法及重组cho细胞 |
CN113430227A (zh) * | 2020-03-23 | 2021-09-24 | 佛山汉腾生物科技有限公司 | 制备稳定细胞池的方法、蛋白表达方法及试剂盒 |
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Effective date of registration: 20191203 Address after: Room 511, floor 5, building a, Central Europe center, No. 2, South Lingnan Avenue, Lecong Town, Shunde District, Foshan City, Guangdong Province Applicant after: Foshan hanteng Biotechnology Co., Ltd Applicant after: Cantonbio Co., Ltd. Address before: 510000, Guangdong International Biological Island, Guangzhou Development Zone, No. three, No. 12, road third, layer 302 Applicant before: Cantonbio Co., Ltd. |
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Denomination of invention: Methods of improving transient transfection and stable protein expression in insect cells Effective date of registration: 20210120 Granted publication date: 20200407 Pledgee: Shunde Guangdong rural commercial bank Limited by Share Ltd. Daliang branch Pledgor: Foshan hanteng Biotechnology Co.,Ltd.|CANTONBIO Co.,Ltd. Registration number: Y2021980000562 |