CN107232092A - A kind of production method of loach pseudo-milter - Google Patents
A kind of production method of loach pseudo-milter Download PDFInfo
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- CN107232092A CN107232092A CN201710383539.3A CN201710383539A CN107232092A CN 107232092 A CN107232092 A CN 107232092A CN 201710383539 A CN201710383539 A CN 201710383539A CN 107232092 A CN107232092 A CN 107232092A
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- loach
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- 241000252185 Cobitidae Species 0.000 title claims abstract description 94
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 239000003163 gonadal steroid hormone Substances 0.000 claims abstract description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 230000008771 sex reversal Effects 0.000 claims abstract description 30
- 241001247197 Cephalocarida Species 0.000 claims abstract description 26
- 239000008188 pellet Substances 0.000 claims abstract description 20
- 239000012528 membrane Substances 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 16
- 238000012545 processing Methods 0.000 claims abstract description 15
- 230000033228 biological regulation Effects 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 241000251468 Actinopterygii Species 0.000 claims abstract description 9
- 238000011534 incubation Methods 0.000 claims abstract description 9
- 230000007704 transition Effects 0.000 claims abstract description 9
- 230000035479 physiological effects, processes and functions Effects 0.000 claims abstract description 7
- 230000000366 juvenile effect Effects 0.000 claims abstract description 5
- 238000012360 testing method Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 17
- GCKMFJBGXUYNAG-UHFFFAOYSA-N 17alpha-methyltestosterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C)(O)C1(C)CC2 GCKMFJBGXUYNAG-UHFFFAOYSA-N 0.000 claims description 13
- GCKMFJBGXUYNAG-HLXURNFRSA-N Methyltestosterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@](C)(O)[C@@]1(C)CC2 GCKMFJBGXUYNAG-HLXURNFRSA-N 0.000 claims description 13
- 229960001566 methyltestosterone Drugs 0.000 claims description 13
- 238000010129 solution processing Methods 0.000 claims description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 8
- 210000004907 gland Anatomy 0.000 claims description 8
- 230000001568 sexual effect Effects 0.000 claims description 8
- 230000004069 differentiation Effects 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 210000001672 ovary Anatomy 0.000 claims description 6
- 241000238582 Artemia Species 0.000 claims description 5
- 230000009027 insemination Effects 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 241000700141 Rotifera Species 0.000 claims description 4
- 230000009977 dual effect Effects 0.000 claims description 4
- 210000002969 egg yolk Anatomy 0.000 claims description 4
- 235000013601 eggs Nutrition 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 229960000583 acetic acid Drugs 0.000 claims description 3
- 239000012362 glacial acetic acid Substances 0.000 claims description 3
- 239000007928 intraperitoneal injection Substances 0.000 claims description 3
- 210000000006 pectoral fin Anatomy 0.000 claims description 3
- 150000002576 ketones Chemical class 0.000 claims description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- 210000001550 testis Anatomy 0.000 claims description 2
- 150000002746 methyltestosterones Chemical class 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 238000003822 preparative gas chromatography Methods 0.000 description 3
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000002149 gonad Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
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- 235000013372 meat Nutrition 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a kind of production method of loach pseudo-milter, comprise the following steps：1) hatch；2) temperature adjusting sex reversal：After loach fry incubation of membrane, water temperature is gradually risen to 27~32 DEG C and cultivates loach fry to juvenile fish by loach fry sex-ridge before being formed；3) male sex hormone regulation and control sex reversal：Gradually transition method is continuously fed for artemia larvae and the pellet mixing handled after loach fry incubation of membrane with male sex hormone solution；4) it is normal to raise：Water temperature is adjusted to normal water temperature, and feeds the pellet without the processing of male sex hormone solution, raising rejects XX ♀ rauns when can extremely distinguish male and female；5) test cross：XX ♂ physiology milter, XY ♂ milters are raised to sexal maturity and mated respectively with XX ♀ rauns, it is XX ♂ pseudo-milters to filter out the male parent that filial generation is 100%XX ♀ rauns.The present invention regulates and controls sex reversal by temperature and male sex hormone collective effect so that the survival rate and sex reversal rate of loach fry can reach optimum state simultaneously, and then available for large-scale production gynoecial loaches.
Description
Technical field
The present invention relates to the sex control technology of fish, belong to enetics-breeding in fish technical field, and in particular to one
The production method of loach pseudo-milter is planted, applied to scale gynoecial loaches Seedling production.
Background technology
Loach meat delicious, is of high nutritive value, wide market, is the important fish of freshwater aquiculture.Supported in identical
Under the conditions of growing, female loach is faster than the growth of milter born of the same parents, gain in weight is big.Cultivation gynoecial loaches will significantly improve cultured output and
Economic benefit.
In production application, the common rauns of XX ♀ and XX ♂ pseudo-milters can be used（It is female in heredity, physiologically male）
Artificial insemination, biological control obtains unisexuality raun.And XX ♂ pseudo-milters are obtained there is several methods that available：Such as temperature control,
Male sex hormone directly induces sex reversal, gynogenesis and male sex hormone induction to be combined, and every kind of fish use different methods
Caused survival rate and sex reversal rate are differed.The method for obtaining pseudo-milter on loach at present has gynogenesis to combine male
Hormone sex reversal, but survival rate and sex reversal rate can not reach optimum state simultaneously（Liu Tongming etc., 2004）；Some scholars are led to
Excess temperature controls the Gonad Differentiation of loach, and male ratio only reaches 86%-90% more than 30 DEG C（Nan Ping etc., 2007；Chen Yuhong etc.,
2007）, it is impossible to applied to full raun large-scale production.
The content of the invention
The purpose of the present invention is to overcome the survival rate and sex reversal rate of existing loach pseudo-milter production can not be while reaching most
The problem of good state.
Therefore, the embodiments of the invention provide a kind of production method of loach pseudo-milter, comprising the following steps：
1) hatch：The Fertilized Eggs of Loach of normal artificial insemination is selected, is placed in 24~26 DEG C of water temperatures and hatches；
2) temperature adjusting sex reversal：After loach fry incubation of membrane, loach fry sex-ridge formed before by water temperature gradually rise to
27~32 DEG C, and loach fry is cultivated to juvenile fish under this water temperature condition；
3) male sex hormone regulation and control sex reversal：First using yolk or wheel animalcule as open-mouthed bait after loach fry incubation of membrane, then adopt
Gradually transition method is continuously fed 60~80 days for artemia larvae and pellet mixing after being handled with male sex hormone solution；
4) it is normal to raise：Through step 2) and step 3) after dual regulation and control sex reversal processing, water temperature is adjusted to normal water temperature, and is thrown
The pellet without the processing of male sex hormone solution is fed, switchs to normal raise and rejects XX ♀ when male and female can be extremely distinguished by pectoral fin shape
Raun, preserves XX ♂ physiology milter, XY ♂ milters standby；
5) test cross：XX ♂ physiology milter, XY ♂ milters are raised to sexal maturity, then mated respectively with XX ♀ rauns, parent and get
To fry be separated raising, fry was supported to 2~3 months, and filial generation is filtered out for 100% XX by fry male ratio detection method
The colony of ♀ rauns, its male parent is XX ♂ pseudo-milters.
Further, the step 2) reclaimed water temperature rise at high speed for daily water temperature raise 0.5~1.5 DEG C, 5 sky and water temperature rises are extremely
27~32 DEG C.
Further, the step 3) in using the artemia larvae after the processing of male sex hormone solution and pellet mixing by
The detailed process that gradual transition method is continuously fed is as follows：The 8th~28 day after loach fry membrane, after being handled with male sex hormone solution
Artemia larvae is fed, and is fed daily 4 times, and 6 hours are separated by every time；The 29th~30 day after membrane, after being handled with male sex hormone solution
Artemia larvae and male sex hormone solution processing after feed mixing feed, feed daily 4 times, 6 hours be separated by every time；Use afterwards
Feedstuff feeding after the processing of male sex hormone solution, feeds 3 times daily.
Further, the artemia larvae after male sex hormone solution processing be use artemia larvae first 150~
Soaked 1 hour in 300IU Methyltestosterone；Feed after the processing of male sex hormone solution is to dissolve Methyltestosterone alcohol
After be sprayed on pellet and dry.
Further, Methyltestosterone content is that 1kg pellets contain 20~40mg methyl testis in the pellet
Ball ketone.
Further, after the male sex hormone solution processing when artemia larvae and feed mixing is fed, both quality
Than pressing 7 successively:1、3:1、5:3、1:1、3:5、1:3、1:7 gradually feed.
Further, every morning, noon and afternoon respectively feed during feedstuff feeding after male sex hormone solution processing
Once, feeding volume was eaten up in 30 minutes and is defined.
Further, the step 5) in fry male ratio detection method detailed process it is as follows：Toward loach intraperitoneal injection
By methanol and glacial acetic acid in mass ratio 3:After 1 fixer prepared, dissection loach under anatomical lens or to visually observe differentiation male
Fish or raun, in thin-line-shaped, two sexual glands are evenly distributed in the spermary that internal both sides are male loach, thicker than spermary, are in
Bulk, two pieces of sexual glands connect together as the ovary of female loach.
Compared with prior art, beneficial effects of the present invention：
(1) production method for this loach pseudo-milter that the present invention is provided is inverse by temperature and male sex hormone collective effect control
Turn, the survival rate of loach fry is to more than 70%, and sex reversal rate reaches more than 97%, meanwhile, compared to existing gynogenesis
With reference to male sex hormone sex reversal, damage of the Artificial gynogenesis to embryonated egg inhereditary material is reduced, pseudo-milter is improved
The pick-up rate of raun.
(2) production method for this loach pseudo-milter that the present invention is provided uses male when male sex hormone regulates and controls sex reversal
The method that artemia larvae and feed after hormone solution processing mix gradually transition is fed, it is to avoid long-term male of only taking swashs
The artemia larvae of plain solution processing is it is possible that malnutrition, improves the survival rate of loach fry.
(3) in the production method of this loach pseudo-milter that the present invention is provided fry male ratio detection method compared to existing
Fry or sexual gland fixed with Bo Enshi liquid, do paraffin section, for dyeing observation, simple to operate, cost is low, can be in fry
Scene is completed, applied widely.
Brief description of the drawings
Fig. 1 is the step flow diagram of the production method of loach pseudo-milter of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in this real inventive embodiments, the technical scheme in the embodiment of the present invention is carried out it is clear,
It is fully described by, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art obtained under the premise of creative work is not made it is all its
Its embodiment, belongs to the scope of protection of the invention.
As shown in figure 1, present embodiments providing a kind of production method of loach pseudo-milter, comprise the following steps：
1) hatch：The Fertilized Eggs of Loach of normal artificial insemination is selected, is placed in 24~26 DEG C of water temperatures and hatches.
2) temperature adjusting sex reversal：After loach fry incubation of membrane, loach fry sex-ridge gradually rises water temperature before being formed
Up to 27~32 DEG C, and loach fry is cultivated to juvenile fish under this water temperature condition.
Because under 23~25 DEG C of water temperature conditions, loach fry the 25th day or so ovary after membrane starts after differentiation, membrane
33 days or so spermaries start differentiation.And the height of temperature also influences the time of Gonad Differentiation, water temperature is higher, PGCs (reproduction cell)
The time for migrating into sex-ridge is more early, under 27~32 DEG C of water temperature conditions, and the fry sex-ridge of 10 days has been formed after membrane, and
There is a small amount of PGCs to migrate into sex-ridge, PGCs enters after sex-ridge, and original sexual gland gradually starts differentiation, then buds into essence
Nest or ovary.Therefore, under above-mentioned cultivation water temperature condition, the effective initial time for inducing loach sex reversal should be after hatching 10
Before it (before i.e. fry sex-ridge is formed), preferred water temperature ramp-up rate is daily water temperature rise 0.5~1.5 in the present embodiment
DEG C, 5 sky and water temperature rises are to 27~32 DEG C.And the rise of water temperature can be suppressed the activity of the aromatizing enzyme of loach fry, it is impossible to
Catalysis testosterone changes into estradiol（Estrogen）, so as to prevent loach juvenile fish to break up to female, improve the male of loach fry
Rate.
3) male sex hormone regulation and control sex reversal：First using yolk or wheel animalcule as open-mouthed bait after loach fry incubation of membrane,
Using the artemia larvae after the processing of male sex hormone solution and pellet mixing, gradually transition method is continuously fed 60~80 days again.
While temperature adjusting sex reversal, first after loach fry incubation of membrane 3 days using yolk or wheel animalcule as opening
Mouth bait is open, afterwards the 8th~28 day after loach fry membrane, and the artemia larvae after being soaked using Methyltestosterone is thrown
Hello, feed daily 4 times, 6 hours are separated by every time；The 29th~30 day after membrane, artemia larvae and spray after being soaked with Methyltestosterone
The feed of Methyltestosterone is spilt successively by both mass ratioes 7:1、3:1、5:3、1:1、1:1、3:5、1:3、1:7 are fed, often
It is fed 4 times, and 6 hours are separated by every time；Fed afterwards with the feed of sprinkling Methyltestosterone, daily 7:00、12:00、18:
00 feeds 3 times, and feeding volume was eaten up in 30 minutes and is defined.Wherein, Methyltestosterone immersion artemia larvae is specifically by artemia children
Body is first in 150~300 IU Methyltestosterone（MT）Middle immersion 1 hour, then suction filtration take out the artemia larvae of enrichment male sex hormone
Feed loach fry；And the feed for having sprayed Methyltestosterone is to be sprayed at pellet after Methyltestosterone is dissolved with alcohol
On dry after feed loach fry again, content is that 1kg pellets contain 20~40mgMT.
First fed in the present embodiment with male sex hormone immersion artemia larvae, be, because bore is smaller after loach opening, not have
Suitable pellet, and Mortality phenomenon is there may be due to having soaked the artemia larvae of male sex hormone, later stage loach
Nutritional need is also different, and the loach for the artemia larvae for having soaked male sex hormone is only taken for a long time it is possible that malnutritive existing
As, loach survival rate is influenceed, therefore, the present embodiment first feeds a period of time with the artemia larvae for having soaked male sex hormone, then slowly
The slow pellet for being transitioned into sprinkling male sex hormone, meanwhile, during loach fry turns food, using the artemia children of male sex hormone
The method that the feed mixing of body and sprinkling male sex hormone carries out gradually transition, makes loach fry gradually adapt to the conversion of feed, can
The survival rate of loach fingerling is effectively improved, and is shown experimentally that, is fed always using the artemia larvae for having soaked male sex hormone
The survival rate of loach fry be no more than 60%, it is middle that the artemia larvae for having soaked male sex hormone is directly converted into sprinkling male
The feed of hormone is fed, without artemia larvae and feed mixed transition, and the success rate that loach fry turns food is very low, loach
The survival rate of fry is no more than 20%.
Show additionally by experiment, through step 2) and step 3) temperature and male sex hormone dual regulation and control sex reversal processing
Afterwards, the survival rate of loach fry is to more than 70%, and sex reversal rate reaches more than 97%；And use single temperature adjusting sex reversal training
The sex reversal rate for educating loach fry is less than 90%, and the survival rate that loach fry is cultivated in single use male sex hormone regulation and control sex reversal is small
In 60%.
4) it is normal to raise：Through step 2) and step 3) after dual regulation and control sex reversal processing, water temperature is adjusted to normal water temperature
（22~25 DEG C）, and the pellet without the processing of male sex hormone solution is fed, switching to normal raise extremely can be by pectoral fin shape area
XX ♀ rauns are rejected when dividing male and female, XX ♂ physiology milter, XY ♂ milters are preserved standby.
5) test cross：XX ♂ physiology milter, XY ♂ milters are raised to sexal maturity, then mated respectively with XX ♀ rauns, parent
Raising is separated with obtained fry, fry was supported to 2~3 months, and filtering out filial generation by fry male ratio detection method is
The colony of 100% XX ♀ rauns, its male parent is loach XX ♂ pseudo-milters.
Fry male ratio detection method in the present embodiment：Toward loach intraperitoneal injection by methanol and glacial acetic acid in mass ratio 3:
After 1 fixer prepared, its spermary or ovary can gradually bleach, and under anatomical lens or be visually observed after dissection, distinguish milter
Or raun：The spermary of general male loach is in thin-line-shaped, and two sexual glands are evenly distributed in internal both sides, and the ovum of female loach
Nest is comparatively thicker than spermary, in bulk, and two pieces of left and right ovary connects together.The fry male ratio detection method compared to
Existing conventional method fixes fry or sexual gland with Bo Enshi liquid, does paraffin section, for dyeing observation, it is simple to operate, into
This is low, can be completed in fry.
In addition, the present embodiment to be produced to the loach pseudo-milter confirmed by group expanding and gynoecial loaches breeding population is set up
Large-scale production gynoecial loaches, detailed process is as follows：
Group expanding：Take the above-mentioned XX ♂ pseudo-milters filtered out and XX ♀ rauns 1:1 artificial insemination produces the 100% female fries of XX ♀, takes two
/ mono- female fries of XX ♀ press above-mentioned steps 2) and step 3) combination temperature and the common control of male sex hormone reversed as XX ♂
Pseudo-milter, the remaining female fries of XX ♀ continue to cultivate.It is wherein preferred, by different family gynoecial loaches in identical cultivating condition
Lower cultivation, carries out growth contrast, and the fireballing family of growth selection is used for the foundation of gynoecial loaches breeding population.
The foundation of gynoecial loaches breeding population：The XX ♀ rauns for the same family that group expanding of learning from else's experience is obtained and XX ♂ pseudo-milters are handed over
Match somebody with somebody, its filial generation bred is 100%XX ♀ rauns, can scale acquisition gynoecial loaches by common breeding way.
In summary, the production method for this loach pseudo-milter that the present invention is provided is made jointly by temperature and male sex hormone
With regulation and control sex reversal so that the survival rate and sex reversal rate of loach fry can reach optimum state simultaneously, and then available for scale
Metaplasia produces gynoecial loaches.
It is exemplified as above be only to the present invention for example, do not constitute the limitation to protection scope of the present invention, it is all
It is to be belonged to the same or analogous design of the present invention within protection scope of the present invention.
Claims (8)
1. a kind of production method of loach pseudo-milter, it is characterised in that：Comprise the following steps：
1) hatch：The Fertilized Eggs of Loach of normal artificial insemination is selected, is placed in 24~26 DEG C of water temperatures and hatches；
2) temperature adjusting sex reversal：After loach fry incubation of membrane, loach fry sex-ridge formed before by water temperature gradually rise to
27~32 DEG C, and loach fry is cultivated to juvenile fish under this water temperature condition；
3) male sex hormone regulation and control sex reversal：First using yolk or wheel animalcule as open-mouthed bait after loach fry incubation of membrane, then adopt
Gradually transition method is continuously fed 60~80 days for artemia larvae and pellet mixing after being handled with male sex hormone solution；
4) it is normal to raise：Through step 2) and step 3) after dual regulation and control sex reversal processing, water temperature is adjusted to normal water temperature, and is thrown
The pellet without the processing of male sex hormone solution is fed, switchs to normal raise and rejects XX ♀ when male and female can be extremely distinguished by pectoral fin shape
Raun, preserves XX ♂ physiology milter, XY ♂ milters standby；
5) test cross：XX ♂ physiology milter, XY ♂ milters are raised to sexal maturity, then mated respectively with XX ♀ rauns, parent and get
To fry be separated raising, fry was supported to 2~3 months, and filial generation is filtered out for 100% XX by fry male ratio detection method
The colony of ♀ rauns, its male parent is XX ♂ pseudo-milters.
2. the production method of loach pseudo-milter as claimed in claim 1, it is characterised in that：The step 2) reclaimed water temperature rise is at a high speed
Spend and raise 0.5~1.5 DEG C for daily water temperature, 5 sky and water temperature rises are to 27~32 DEG C.
3. the production method of loach pseudo-milter as claimed in claim 1, it is characterised in that：The step 3) it is middle sharp using male
Artemia larvae and the pellet mixing detailed process that gradually transition method is continuously fed after plain solution processing is as follows：Loach fry
The 8th~28 day after membrane, the artemia larvae after being handled with male sex hormone solution is fed, and feeds daily 4 times, 6 hours are separated by every time；
The 29th~30 day after membrane, the feed after artemia larvae and the processing of male sex hormone solution after being handled with male sex hormone solution is mixed
Feed, feed daily 4 times, 6 hours are separated by every time；Afterwards with the feedstuff feeding after the processing of male sex hormone solution, 3 are fed daily
It is secondary.
4. the production method of loach pseudo-milter as claimed in claim 3, it is characterised in that：After the male sex hormone solution processing
Artemia larvae be use by artemia larvae first in 150~300IU Methyltestosterone soak 1 hour；Male sex hormone solution
Feed after processing is to be sprayed on pellet to dry after Methyltestosterone is dissolved with alcohol.
5. the production method of loach pseudo-milter as claimed in claim 4, it is characterised in that：Methyl testis in the pellet
Ketone content is that 1kg pellets contain 20~40mg Methyltestosterones.
6. the production method of loach pseudo-milter as claimed in claim 3, it is characterised in that：After the male sex hormone solution processing
Artemia larvae and feed mixing when feeding, both mass ratioes press 7 successively:1、3:1、5:3、1:1、3:5、1:3、1:7 gradually
Feed.
7. the production method of loach pseudo-milter as claimed in claim 3, it is characterised in that：After the male sex hormone solution processing
Feedstuff feeding when every morning, noon and afternoon respectively feed once, feeding volume was eaten up in 30 minutes and is defined.
8. the production method of loach pseudo-milter as claimed in claim 1, it is characterised in that：The step 5) in fry male ratio
Detection method detailed process is as follows：Toward loach intraperitoneal injection by methanol and glacial acetic acid in mass ratio 3:1 fixer prepared, solution
Differentiation milter or raun are cutd open after loach under anatomical lens or visually observe, in thin-line-shaped, two sexual glands are evenly distributed in body
Interior both sides are the spermary of male loach, and thicker than spermary, in bulk, two pieces of sexual glands connect together as the ovary of female loach.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710383539.3A CN107232092A (en) | 2017-05-26 | 2017-05-26 | A kind of production method of loach pseudo-milter |
Applications Claiming Priority (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107821244A (en) * | 2017-11-16 | 2018-03-23 | 浙江省海洋水产研究所 | A kind of ratio of female high little yellow croaker breeding method and feedstuff feeding device |
| CN113475433A (en) * | 2021-08-12 | 2021-10-08 | 江苏省淡水水产研究所 | Method for breeding channel catfish pseudo female fish |
| CN115024251A (en) * | 2022-07-04 | 2022-09-09 | 淮阴师范学院 | Rapid acquisition method for full-male Pseudobagrus ussuriensis |
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| CN106259089A (en) * | 2016-08-15 | 2017-01-04 | 安徽省农业科学院水产研究所 | A kind of method of large-scale production gynoecial loaches |
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| CN103548740A (en) * | 2013-11-07 | 2014-02-05 | 武汉百瑞生物技术有限公司 | Method for producing gynoecial loaches |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821244A (en) * | 2017-11-16 | 2018-03-23 | 浙江省海洋水产研究所 | A kind of ratio of female high little yellow croaker breeding method and feedstuff feeding device |
| CN107821244B (en) * | 2017-11-16 | 2019-09-24 | 浙江省海洋水产研究所 | A kind of little yellow croaker breeding method and feedstuff feeding device that ratio of female is high |
| CN113475433A (en) * | 2021-08-12 | 2021-10-08 | 江苏省淡水水产研究所 | Method for breeding channel catfish pseudo female fish |
| CN113475433B (en) * | 2021-08-12 | 2024-02-27 | 江苏省淡水水产研究所 | Method for cultivating channel catfish pseudo-female fish |
| CN115024251A (en) * | 2022-07-04 | 2022-09-09 | 淮阴师范学院 | Rapid acquisition method for full-male Pseudobagrus ussuriensis |
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