CN107227374A - 一种基于荧光定量pcr鉴定啮齿类螺杆菌的引物、试剂盒及鉴定方法 - Google Patents
一种基于荧光定量pcr鉴定啮齿类螺杆菌的引物、试剂盒及鉴定方法 Download PDFInfo
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- CN107227374A CN107227374A CN201710646881.8A CN201710646881A CN107227374A CN 107227374 A CN107227374 A CN 107227374A CN 201710646881 A CN201710646881 A CN 201710646881A CN 107227374 A CN107227374 A CN 107227374A
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- helicobacter
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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CN201710646881.8A CN107227374A (zh) | 2017-08-01 | 2017-08-01 | 一种基于荧光定量pcr鉴定啮齿类螺杆菌的引物、试剂盒及鉴定方法 |
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CN201710646881.8A CN107227374A (zh) | 2017-08-01 | 2017-08-01 | 一种基于荧光定量pcr鉴定啮齿类螺杆菌的引物、试剂盒及鉴定方法 |
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CN107227374A true CN107227374A (zh) | 2017-10-03 |
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CN201710646881.8A Pending CN107227374A (zh) | 2017-08-01 | 2017-08-01 | 一种基于荧光定量pcr鉴定啮齿类螺杆菌的引物、试剂盒及鉴定方法 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014087055A1 (en) * | 2012-12-05 | 2014-06-12 | Amplidiag Oy | Method for detecting helicobacter pylori dna in a stool sample |
CN105039517A (zh) * | 2015-06-04 | 2015-11-11 | 广东省实验动物监测所 | 一种鉴别5种啮齿动物螺杆菌的pcr-hrm引物和方法 |
CN105154551A (zh) * | 2015-09-18 | 2015-12-16 | 广东省实验动物监测所 | 胆汁螺杆菌的实时荧光定量pcr检测方法 |
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2017
- 2017-08-01 CN CN201710646881.8A patent/CN107227374A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014087055A1 (en) * | 2012-12-05 | 2014-06-12 | Amplidiag Oy | Method for detecting helicobacter pylori dna in a stool sample |
CN105039517A (zh) * | 2015-06-04 | 2015-11-11 | 广东省实验动物监测所 | 一种鉴别5种啮齿动物螺杆菌的pcr-hrm引物和方法 |
CN105154551A (zh) * | 2015-09-18 | 2015-12-16 | 广东省实验动物监测所 | 胆汁螺杆菌的实时荧光定量pcr检测方法 |
Non-Patent Citations (3)
Title |
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NAGAMINE,C.M.等: "Helicobacter sp. MIT10-5753 16S ribosomal RNA gene,partial sequence,GenBank:KP289288.1", 《NCBI GENBANK》 * |
SUNLIAN FENG等: "Differential Detection of Five Mouse-Infecting Helicobacter Species by Multiplex PCR", 《CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY》 * |
李瑞娇等: "啮齿动物螺杆菌多重PCR检测方法的建立", 《中国比较医学杂志》 * |
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Inventor after: Zhang Quan Inventor after: Feng Jie Inventor after: Sun Jingtou Inventor after: Xie Lingzhi Inventor after: Yu Ning Inventor after: Qian Miao Inventor before: Zhang Quan Inventor before: Sun Jingtou Inventor before: Xie Lingzhi Inventor before: Yu Ning Inventor before: Qian Miao |
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Application publication date: 20171003 |