CN107216179A - A kind of cultivation soil of callistemon rigidus nursery stock - Google Patents
A kind of cultivation soil of callistemon rigidus nursery stock Download PDFInfo
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- CN107216179A CN107216179A CN201710519875.6A CN201710519875A CN107216179A CN 107216179 A CN107216179 A CN 107216179A CN 201710519875 A CN201710519875 A CN 201710519875A CN 107216179 A CN107216179 A CN 107216179A
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- callistemon rigidus
- saccharomycete
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to agricultural technology field, and in particular to a kind of cultivation soil of callistemon rigidus nursery stock, is prepared into according to percent by weight including following raw material：5% 10% yellow sands, 5% 10% peat, 5% 10% Extracted From Oil-tea-cakes, 5% 10% asters, 3% 8% ground phosphate rock, 5% 10% potato tubers, 10% 20% mushroom leftover bits and pieces, 5% 10% bone meal, 10% 20% maize leaves, 5% 10% orange peels, 1% 3% chitosans, 0.5% 1.5% saccharomycete, 2% 5% lactic acid bacterias, 1% 5% lactobacillus acidophilus, 3% 5% Bifidobacteriums, 2% 3% bacillus licheniformis.The present invention has nutrient complete, is easily bloomed after plantation, and flowering time is more lasting, and flowers are more close, improves its ornamental value, and peasant cultivates the callistemon rigidus nursery stock of soil cultivation with higher economic value using the application.
Description
【Technical field】
The present invention relates to agricultural technology field, and in particular to a kind of cultivation soil of callistemon rigidus nursery stock.
【Background technology】
Callistemon rigidus (Callistemon rigidus R.Br.) is also known as bottle brush subtree, red bottle brush, treasure tree etc., is peach gold
The evergreen shrubs or dungarunga of ma section, callistemon rigidus belong to heliophilous species, like warm, moist climate, are resistant to burning sun heat, originate in Australia big
Leah.Leaf lanceolar, does not wither throughout the year like Chinese podocarpus branchlet and leaf, evergreen all the year round.Spend dense, consor is in top, the month at florescence 6-8, flower-shape pole
For it is peculiar be in spike, it is and beautiful in colour, can arrange flowers, while being also that garden is seen flower, the preferred of trade landscape, subdistrict greening and set
Kind, high-grade potted landscape is made after can also making windbreak, afforestation forest, or potted plant trimming shaping.China introduces existing one-hundred-year history, China
Taiwan, Guangdong, Guangxi, Fujian, zhejiang and other places have cultivation.Red dried layer is adapted to yard beautification, be senior yard beautification see flower tree,
Shade tree, gardens tree, landscape tree, can also make windbreak, cut-flower or large-scale potted plant, and can pruning turn into noble potted landscape.
But if the cultivation of callistemon rigidus is bad, it is easy to there is malnutritive scarce nutrient, callistemon rigidus is difficult to bloom
Or flower amount is less or the phenomenon such as flower is small, influences ornamental value.
【The content of the invention】
The goal of the invention of the present invention is：For it is above-mentioned the problem of, the present invention provides a kind of cultivation soil of callistemon rigidus nursery stock,
It is complete with nutrient, easily bloomed after plantation, and flowering time is more lasting, flowers are more close, improve its ornamental value,
The callistemon rigidus nursery stock that peasant cultivates soil cultivation using the application has higher economic value.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of cultivation soil of callistemon rigidus nursery stock, is prepared into according to percent by weight including following raw material：5%-10% is yellow
Sand, 5%-10% peat, 5%-10% Extracted From Oil-tea-cakes, 5%-10% asters, 3%-8% ground phosphate rock, 5%-10% potato tubers,
10%-20% mushrooms leftover bits and pieces, 5%-10% bone meal, 10%-20% maize leaves, 5%-10% orange peels, 1%-3% shells gather
Sugar, 0.5%-1.5% saccharomycete, 2%-5% lactic acid bacterias, 1%-5% lactobacillus acidophilus, 3%-5% Bifidobacteriums, 2%-3%
Bacillus licheniformis.
It is preferred that, a kind of cultivation soil of callistemon rigidus nursery stock is prepared into according to percent by weight including following raw material：6%-
9% yellow sand, 6%-9% peat, 6%-8% Extracted From Oil-tea-cakes, 6%-8% asters, 4%-7% ground phosphate rock, 6%-8% potato balls
Stem, 12%-18% mushrooms leftover bits and pieces, 6%-9% bone meal, 12%-18% maize leaves, 7%-9% orange peels, 1.5%-2.5%
Chitosan, 0.7%-1.2% saccharomycete, 2.5%-4.5% lactic acid bacterias, 1.5%-4.0% lactobacillus acidophilus, 3.5%-4% are double
Discrimination bacillus, 2%-3% bacillus licheniformis.
More preferred, a kind of cultivation soil of callistemon rigidus nursery stock is prepared into according to percent by weight including following raw material：
7% yellow sand, 7% peat, 7% Extracted From Oil-tea-cake, 7% aster, 5% ground phosphate rock, 7% potato tubers, 15% mushroom leftover bits and pieces, 7%
The newborn bar of bone meal, 14% maize leaves, 8% orange peel, 2.0% chitosan, 1.0% saccharomycete, 3.5% lactic acid bacteria, 3.2% acidophilus
Bacterium, 3.8% Bifidobacterium, 2.5% bacillus licheniformis.
A kind of preparation method of the cultivation soil of callistemon rigidus nursery stock as described above, is comprised the following steps that：
A, saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis be respectively put into LB culture mediums
Activation 2-3 hours is carried out, progress culture 60-84h in fluid nutrient medium is then seeded to respectively again, carries out having detected respectively
It is that effective bacteria containing amount is 2.56 × 10 to imitate bacteria containing amount8-3.42×109After individual/mL；
B, take aster rhizome be dried after crush, by circulating ultrasonic extraction element according to comminuted powder and extraction solvent
Oil is 1 according to volume ratio:2 ratio mixing is extracted, and 50-60 degrees Celsius of Extracting temperature, supersonic frequency is 30-40KHz, extracts 2-
5 minutes, circulation was extracted 5 times, 5 liquid was merged after filtering, it is molten that liquid loads solvent recovery 50-60 degrees Celsius of recovery of enrichment facility
Agent, then carries out refined filtration and obtains aster extract solution；
C, orange peel is taken to add water and cellulase, volume ratio is 1:1:0.02-0.05, is digested, then go out enzyme it
Afterwards, enzymolysis liquid is obtained, the pH of enzymolysis liquid is adjusted to 4-6, is extracted with ethyl acetate, organic phase is taken, is added in organic phase
The 1-3wt.% of the weight sodium hydrate aqueous solution such as enter, stir, extracted, aqueous phase is sent into milipore filter by phase of fetching water
Filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtain tangeritin extraction
Thing；
D, potato tubers smashed to pieces, be subsequently added into peat, Extracted From Oil-tea-cake, ground phosphate rock, mushroom leftover bits and pieces, maize leaves and step
The aster that saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis and step B, C after A processing are obtained carries
Take after liquid and the mixing of tangeritin extract, build heap fermentation process and obtain the first fermentation material；
E, the first fermentation material and bone meal, chitosan mixed, obtain bed material, yellow sand is then spread on bed material.
Further illustrate, the condition of the fermentation is to control temperature at 30-32 degrees Celsius.
Further illustrate, the condition of the activation is temperature at 35-38 degrees Celsius.
Further illustrate, the condition of the refined filtration is that pressure is that 10-15MPa, temperature are 30-35 DEG C, CO2Flow 15kg/
H, time are 15 minutes.
Further illustrate, the formula of the fluid nutrient medium is：Sodium acetate 5-8g, sodium acid carbonate 4-6g, ammonium sulfate 6-8g,
Ammonium chloride 3-5g, potassium dihydrogen phosphate 4-7g, potassium phosphate 0.5-1.0g, sodium chloride 0.5-0.8g, magnesium sulfate 0.3-0.5g, calcium chloride
0.3-0.8g, white sugar 5-15g, yeast extract 10-12g, peptone 15-18g, water 1L, are 7.0 with second acid for adjusting pH value.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
The present invention's is native in cultivation, and saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis are through making a living
Change after culture and the culture of effective bacterium, with good capacity of decomposition, fermentation can be promoted to decompose the releases such as the organic matter in raw material big
Secondary element, micro- various amino acid etc., but after effective bacterium is cultivated, strain overlong time, majority, which has been bred, to be arrived
Stationary phase, is next exactly declining period, and effective bacterium will be substantially reduced when carrying out with raw material afterwards and build heap fermentation process
Activity, then the applicant carried out substantial amounts of experimental study from this angle, find to contain in the aster extract added
There are substantial amounts of acidic materials, can be again to saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, the effective bacterium of bacillus licheniformis
Activated, improve their activity, and potato tubers releases substantial amounts of lactic acid afterwards by fermentation, it is further to improve
The activity of the effective bacterium of saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis, and then further improve
Their capacity of decomposition, so that in fermentation process, various a great number of elements, trace element in raw material will be contracted by fast decoupled
The time of bob ferment, in order to, the nitrogen source content in bed material after handling by fermentation is 28.9-35.6%, and carbon source content is
Member in 32.4-41.5%, organic matter 58.6-66.7%, available nitrogen 0.55-0.88%, rapid available phosphorus 2.55-3.65%, bed material
Element can improve the flowering time of callistemon rigidus, cause the density increase of flowers, and the size increase of flower improves its ornamental value.
【Embodiment】
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Embodiment is closed, the present invention is expanded on further.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention,
It is not intended to limit the present invention.
Embodiment 1：
A kind of cultivation soil of callistemon rigidus nursery stock, is prepared into according to percent by weight including following raw material：5% yellow sand, 5%
Peat, 5% Extracted From Oil-tea-cake, 5% aster, 5% ground phosphate rock, 5% potato tubers, 17.5% mushroom leftover bits and pieces, 5% bone meal, 20%
Maize leaves, 5% orange peel, 3% chitosan, 1.5% saccharomycete, 5% lactic acid bacteria, 5% lactobacillus acidophilus, 5% Bifidobacterium,
3% bacillus licheniformis.
A kind of preparation method of the cultivation soil of callistemon rigidus nursery stock as described above, is comprised the following steps that：
A, saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis be respectively put into LB culture mediums
Temperature carried out under 38 degrees Celsius activation 2 hours, be then seeded to respectively again by sodium acetate 5g, sodium acid carbonate 4g, ammonium sulfate 6g,
Ammonium chloride 3g, potassium dihydrogen phosphate 4g, potassium phosphate 0.5g, sodium chloride 0.5g, magnesium sulfate 0.3g, calcium chloride 0.3-0.8g, white sugar 5g,
Yeast extract 10g, peptone 15g, water 1L, are to carry out culture 60h in 7.0 obtained fluid nutrient mediums with second acid for adjusting pH value, point
It Jin Hang not detect that it is that effective bacteria containing amount is 2.56 × 10 to obtain effective bacteria containing amount8After individual/mL；
B, take aster rhizome be dried after crush, by circulating ultrasonic extraction element according to comminuted powder and extraction solvent
Oil is 1 according to volume ratio:2 ratio mixing is extracted, 50 degrees Celsius of Extracting temperature, and supersonic frequency is 30KHz, extracts 2 minutes, follows
Ring is extracted 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery 50 degrees Celsius of recycling designs of enrichment facility, then carried out
Pressure is that 10MPa, temperature are 30 DEG C, CO2Flow 15kg/h, time obtained aster extract solution for the refined filtration of 15 minutes；
C, orange peel is taken to add water and cellulase, volume ratio is 1:1:0.02, digested, then after the enzyme that go out, obtain
To enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, is extracted with ethyl acetate, organic phase is taken, the weight such as addition in organic phase
1wt.% sodium hydrate aqueous solution, stir, extracted, fetch water phase, by aqueous phase feeding milipore filter filtered, then
The penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains tangeritin extract；
D, potato tubers smashed to pieces, be subsequently added into peat, Extracted From Oil-tea-cake, ground phosphate rock, mushroom leftover bits and pieces, maize leaves and step
The aster that saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis and step B, C after A processing are obtained carries
Take after liquid and the mixing of tangeritin extract, build heap control temperature and obtain the first fermentation material in 30 degrees Celsius of fermentation process；
E, the first fermentation material and bone meal, chitosan mixed, obtain bed material, yellow sand is then spread on bed material.
Embodiment 2：
A kind of cultivation soil of callistemon rigidus nursery stock, is prepared into according to percent by weight including following raw material：10% yellow sand,
10% peat, 10% Extracted From Oil-tea-cake, 10% aster, 3% ground phosphate rock, 10% potato tubers, 10% mushroom leftover bits and pieces, 10% bone
Powder, 10% maize leaves, 7.5% orange peel, 1% chitosan, 0.5% saccharomycete, 2% lactic acid bacteria, 1% lactobacillus acidophilus, 3% pair
Discrimination bacillus, 2% bacillus licheniformis.
A kind of preparation method of the cultivation soil of callistemon rigidus nursery stock as described above, is comprised the following steps that：
A, saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis be respectively put into LB culture mediums
Temperature carried out under 38 degrees Celsius activation 3 hours, be then seeded to respectively again by sodium acetate 8g, sodium acid carbonate 6g, ammonium sulfate 8g,
Ammonium chloride 5g, potassium dihydrogen phosphate 7g, potassium phosphate 1.0g, sodium chloride 0.8g, magnesium sulfate 0.5g, calcium chloride 0.8g, white sugar 15g, ferment
Female cream 12g, peptone 18g, water 1L, are to carry out culture 84h in 7.0 obtained fluid nutrient mediums with second acid for adjusting pH value, respectively
Progress detects that it is that effective bacteria containing amount is 3.42 × 10 to obtain effective bacteria containing amount9After individual/mL；
B, take aster rhizome be dried after crush, by circulating ultrasonic extraction element according to comminuted powder and extraction solvent
Oil is 1 according to volume ratio:2 ratio mixing is extracted, 60 degrees Celsius of Extracting temperature, and supersonic frequency is 40KHz, extracts 5 minutes, follows
Ring is extracted 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery 60 degrees Celsius of recycling designs of enrichment facility, then carried out
Pressure is that 15MPa, temperature are 35 DEG C, CO2Flow 15kg/h, time obtained aster extract solution for the refined filtration of 15 minutes；
C, orange peel is taken to add water and cellulase, volume ratio is 1:1:0.05, digested, then after the enzyme that go out, obtain
To enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 6, is extracted with ethyl acetate, organic phase is taken, the weight such as addition in organic phase
3wt.% sodium hydrate aqueous solution, stir, extracted, fetch water phase, by aqueous phase feeding milipore filter filtered, then
The penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains tangeritin extract；
D, potato tubers smashed to pieces, be subsequently added into peat, Extracted From Oil-tea-cake, ground phosphate rock, mushroom leftover bits and pieces, maize leaves and step
The aster that saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis and step B, C after A processing are obtained carries
Take after liquid and the mixing of tangeritin extract, build heap control temperature and obtain the first fermentation material in 32 degrees Celsius of fermentation process；
E, the first fermentation material and bone meal, chitosan mixed, obtain bed material, yellow sand is then spread on bed material.
Embodiment 3：
A kind of cultivation soil of callistemon rigidus nursery stock, is prepared into according to percent by weight including following raw material：7% yellow sand, 7%
Peat, 7% Extracted From Oil-tea-cake, 7% aster, 5% ground phosphate rock, 7% potato tubers, 15% mushroom leftover bits and pieces, 7% bone meal, 14% jade
Meter Ye, 8% orange peel, 2.0% chitosan, 1.0% saccharomycete, 3.5% lactic acid bacteria, 3.2% lactobacillus acidophilus, 3.8% bifid
Bacillus, 2.5% bacillus licheniformis
A kind of preparation method of the cultivation soil of callistemon rigidus nursery stock as described above, is comprised the following steps that：
A, saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis be respectively put into LB culture mediums
Temperature carried out under 35-38 degrees Celsius activation 2-3 hours, be then seeded to respectively again by sodium acetate 5-8g, sodium acid carbonate 4-6g,
Ammonium sulfate 6-8g, ammonium chloride 3-5g, potassium dihydrogen phosphate 4-7g, potassium phosphate 0.5-1.0g, sodium chloride 0.5-0.8g, magnesium sulfate 0.3-
0.5g, calcium chloride 0.3-0.8g, white sugar 5-15g, yeast extract 10-12g, peptone 15-18g, water 1L, be with second acid for adjusting pH value
Culture 60-84h is carried out in 7.0 obtained fluid nutrient mediums, carries out detecting that it is that effective bacteria containing amount is to obtain effective bacteria containing amount respectively
2.56×108-3.42×109After individual/mL；
B, take aster rhizome be dried after crush, by circulating ultrasonic extraction element according to comminuted powder and extraction solvent
Oil is 1 according to volume ratio:2 ratio mixing is extracted, and 50-60 degrees Celsius of Extracting temperature, supersonic frequency is 30-40KHz, extracts 2-
5 minutes, circulation was extracted 5 times, 5 liquid was merged after filtering, it is molten that liquid loads solvent recovery 50-60 degrees Celsius of recovery of enrichment facility
Agent, it is that 10-15MPa, temperature are 30-35 DEG C, CO then to carry out pressure2Flow 15kg/h, time obtained purple for the refined filtration of 15 minutes
Garden extract solution；
C, orange peel is taken to add water and cellulase, volume ratio is 1:1:0.02-0.05, is digested, then go out enzyme it
Afterwards, enzymolysis liquid is obtained, the pH of enzymolysis liquid is adjusted to 4-6, is extracted with ethyl acetate, organic phase is taken, is added in organic phase
The 1-3wt.% of the weight sodium hydrate aqueous solution such as enter, stir, extracted, aqueous phase is sent into milipore filter by phase of fetching water
Filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtain tangeritin extraction
Thing；
D, potato tubers smashed to pieces, be subsequently added into peat, Extracted From Oil-tea-cake, ground phosphate rock, mushroom leftover bits and pieces, maize leaves and step
The aster that saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis and step B, C after A processing are obtained carries
Take after liquid and the mixing of tangeritin extract, build heap control temperature and obtain the first fermentation material in 30-32 degrees Celsius of fermentation process；
E, the first fermentation material and bone meal, chitosan mixed, obtain bed material, yellow sand is then spread on bed material.
For the foster callistemon rigidus of the cultivation earth culture using embodiment 1-3, in flowering period, not only flowering time is more longlasting,
Have more compared with prior art 7-12 days, the density increase of flowers, the size increase of flower improves its ornamental value.
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (8)
1. a kind of cultivation soil of callistemon rigidus nursery stock, it is characterised in that：It is prepared into according to percent by weight including following raw material：
5%-10% yellow sands, 5%-10% peat, 5%-10% Extracted From Oil-tea-cakes, 5%-10% asters, 3%-8% ground phosphate rock, 5%-10%
Potato tubers, 10%-20% mushrooms leftover bits and pieces, 5%-10% bone meal, 10%-20% maize leaves, 5%-10% orange peels,
1%-3% chitosans, 0.5%-1.5% saccharomycete, 2%-5% lactic acid bacterias, 1%-5% lactobacillus acidophilus, 3%-5% bifid bars
Bacterium, 2%-3% bacillus licheniformis.
2. a kind of cultivation soil of callistemon rigidus nursery stock according to claim 1, it is characterised in that：According to percent by weight bag
Following raw material is included to be prepared into：6%-9% yellow sands, 6%-9% peat, 6%-8% Extracted From Oil-tea-cakes, 6%-8% asters, 4%-7% phosphorus
Miberal powder, 6%-8% potato tubers, 12%-18% mushrooms leftover bits and pieces, 6%-9% bone meal, 12%-18% maize leaves, 7%-
9% orange peel, 1.5%-2.5% chitosans, 0.7%-1.2% saccharomycete, 2.5%-4.5% lactic acid bacterias, 1.5%-4.0% are thermophilic
Lactobacillus lactis, 3.5%-4% Bifidobacteriums, 2%-3% bacillus licheniformis.
3. a kind of cultivation soil of callistemon rigidus nursery stock according to claim 1, it is characterised in that：According to percent by weight bag
Following raw material is included to be prepared into：7% yellow sand, 7% peat, 7% Extracted From Oil-tea-cake, 7% aster, 5% ground phosphate rock, 7% potato tubers,
15% mushroom leftover bits and pieces, 7% bone meal, 14% maize leaves, 8% orange peel, 2.0% chitosan, 1.0% saccharomycete, 3.5% lactic acid
Bacterium, 3.2% lactobacillus acidophilus, 3.8% Bifidobacterium, 2.5% bacillus licheniformis.
4. a kind of preparation method of the cultivation soil of callistemon rigidus nursery stock according to claim 1, it is characterised in that：Specific steps
It is as follows：
A, by saccharomycete, lactic acid bacteria, lactobacillus acidophilus, Bifidobacterium, bacillus licheniformis be respectively put into LB culture mediums carry out
Activation 2-3 hours, is then seeded to progress culture 60-84h in fluid nutrient medium, is detected effectively contain respectively respectively again
Bacterium amount is that effective bacteria containing amount is 2.56 × 108-3.42×109After individual/mL；
B, take aster rhizome be dried after crush, pressed by circulating ultrasonic extraction element according to comminuted powder with solvent for extraction
It is 1 according to volume ratio:2 ratio mixing is extracted, and 50-60 degrees Celsius of Extracting temperature, supersonic frequency is 30-40KHz, extracts 2-5 points
Clock, circulation is extracted 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery 50-60 degrees Celsius of recycling design of enrichment facility,
Then carry out refined filtration and obtain aster extract solution；
C, orange peel is taken to add water and cellulase, volume ratio is 1:1:0.02-0.05, is digested, then after the enzyme that go out, is obtained
To enzymolysis liquid, the pH of enzymolysis liquid is adjusted to 4-6, is extracted with ethyl acetate, organic phase is taken, the weight such as addition in organic phase
The 1-3wt.% of amount sodium hydrate aqueous solution, is stirred, and is extracted, phase of fetching water, and aqueous phase feeding milipore filter was carried out
Filter, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains tangeritin extract；
D, potato tubers smashed to pieces, be subsequently added at peat, Extracted From Oil-tea-cake, ground phosphate rock, mushroom leftover bits and pieces, maize leaves and step A
The aster that saccharomycete, lactic acid bacteria, lactobacillus acidophilus after reason, Bifidobacterium, bacillus licheniformis and step B, C are obtained is extracted
After liquid and the mixing of tangeritin extract, build heap fermentation process and obtain the first fermentation material；
E, the first fermentation material and bone meal, chitosan mixed, obtain bed material, yellow sand is then spread on bed material.
5. a kind of preparation method of the cultivation soil of callistemon rigidus nursery stock according to claim 4, it is characterised in that：The fermentation
Condition be control temperature at 30-32 degrees Celsius.
6. a kind of preparation method of the cultivation soil of callistemon rigidus nursery stock according to claim 4, it is characterised in that：The activation
Condition be temperature at 35-38 degrees Celsius.
7. a kind of preparation method of the cultivation soil of callistemon rigidus nursery stock according to claim 4, it is characterised in that：The refined filtration
Condition be that pressure is that 10-15MPa, temperature are 30-35 DEG C, CO2Flow 15kg/h, time are 15 minutes.
8. a kind of preparation method of the cultivation soil of callistemon rigidus nursery stock according to claim 4, it is characterised in that：The liquid
The formula of culture medium is：Sodium acetate 5-8g, sodium acid carbonate 4-6g, ammonium sulfate 6-8g, ammonium chloride 3-5g, potassium dihydrogen phosphate 4-7g,
Potassium phosphate 0.5-1.0g, sodium chloride 0.5-0.8g, magnesium sulfate 0.3-0.5g, calcium chloride 0.3-0.8g, white sugar 5-15g, yeast extract
10-12g, peptone 15-18g, water 1L, are 7.0 with second acid for adjusting pH value.
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