CN107212024A - A kind of bactericidal composition of parthenium extract containing oriental wormwood and epoxiconazole - Google Patents
A kind of bactericidal composition of parthenium extract containing oriental wormwood and epoxiconazole Download PDFInfo
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- CN107212024A CN107212024A CN201710481523.6A CN201710481523A CN107212024A CN 107212024 A CN107212024 A CN 107212024A CN 201710481523 A CN201710481523 A CN 201710481523A CN 107212024 A CN107212024 A CN 107212024A
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- epoxiconazole
- oriental wormwood
- parthenium extract
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Biotechnology (AREA)
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Abstract
The present invention relates to a kind of parthenium extract containing oriental wormwood and the bactericidal composition of epoxiconazole, active ingredient is made up of oriental wormwood parthenium extract and epoxiconazole, and the weight ratio of oriental wormwood parthenium extract and epoxiconazole is 20 1:1‑20.The present invention is compounded by oriental wormwood parthenium extract and epoxiconazole, and for the preventing and treating of rice green smut, preventive effect is significantly higher than the preventive effect of single dose.Bactericidal composition of the present invention has synergistic function substantially, and the lasting period is long, it is possible to reduce chemical pesticide usage amount, delays the pathogen resistance to the action of a drug to produce, the features such as to crop safety.
Description
Technical field
The present invention relates to pesticides application technical field, and in particular to a kind of sterilization group of parthenium extract containing oriental wormwood and epoxiconazole
Compound.
Background technology
False smut also known as pseudo- smut, green smut, paddy blossom disease and blue or green powder disease, China are also called " good harvest with Japan
Disease ", is caused by fungi.The disease only occurs in fringe portion, part grain of causing harm.Shape in the performance symptom grain of aggrieved rice green smut
Gradually expanded into mycelia block, it is interior it is coetonium split, expose faint yellow block, i.e. spore seat, after be wrapped in interior coetonium both sides, it is darkly green
Color, first outsourcing thin film, rear rupture, the flat bacterium of black is given birth in scattered blackish green powder, the i.e. chlamydospore of germ, some both sides
Core, exposes to wind and rain easy to fall off.Hebei, the Yangtze river basin and southern each province rice region happen occasionally.
Artemisia capillaris (Artemisia capillaries) is composite family, sagebruss.Early spring harvests seedling.Artemisia capillaris begins to carry
In《Sheng Nong's herbal classic》The tender seedling of Artemisia capillaris.Early spring harvests seedling, removes old stem and impurity is dried.Agglomerating shape is crimped seedling more,
Celadon, it is all close by white hair, it is soft such as suede.Artemisia capillaris has special fragrance, mildly bitter flavor.Be distributed in Jiangxi, Jiangsu, Anhui,
Hubei, Zhejiang, Shaanxi, Hebei, Shandong, Guangdong, Guangxi etc. are saved.Early spring two, the basal leaf of harvesting in March, tender seedling enter with spire
Medicine, Chinese medicine claims " because old ", " oriental wormwood " or " capillary wormwood ", containing chlorogenic acid (chlorogenic acid), coumarin
(scoparone), caffeic acid (caf feic acid) etc., for control liver, courage illness main component.Also contain volatile oil, mainly into
Dividing has family β-pinane child, the old dienone of bacterium (capillione), oriental wormwood stagnant (capillene) and capillarin (capillarin) etc..
Ancient herbal medicine is such as《Sheng Nong's herbal classic》And《Compendium of Materia Medica》Deng record:Main rheumatism, fever and chills, perverse trend thermojunction, jaundice etc..In vitro test
Prove, oriental wormwood decoction is to staphylococcus aureus, corynebacterium diphtheriae, bacillus anthracis, typhoid bacillus, paratyphoid bacillus A, green pus
Bacillus, EHEC, bacterium flexneri, diplococcus meningitidis etc. have different degrees of inhibitory action.
Epoxiconazole (epoxiconazole), molecular formula C17H13ClFN3O.Epoxiconazole is a kind of absorbability triazole type sterilization
Agent, its active component epoxiconazole suppresses the synthesis of germ ergosterol, hinders the formation of germ cell membrane, and epoxiconazole molecule
There is strength compatibility to a kind of fungal enzyme (14-dencthylase), compared with the bactericide being currently known, can more effectively suppress
Germ fungal pathogenses.Epoxiconazole can improve all fourth matter enzymatic activitys of crop, cause the contraction of fungi haustorium, suppress germ intrusion, this
It is epoxiconazole unique characteristic in all triazole type products.Its to a series of cereal crops for example damping-off, powdery mildew,
The multiple diseases of eyeprint disease etc. ten have good preventive and therapeutic effect, and can prevent and treat sugar beet, peanut, rape, lawn, coffee, water
The disease such as rice and fruit tree.Not only have and protect, treat and root out activity well, and lived with interior suction with preferably residual
Property.
Either long-term single use of epoxiconazole or other bactericide is easier to make germ develop immunity to drugs, medicament
Field control effect declines to a great extent.The increase of formulation rate and spraying times causes peasant burden to aggravate and environment again after resistance is produced
Pollution aggravation.To reduce resistance risk and delaying the germ resistance to the action of a drug to produce, the active ingredient for screening the different mechanisms of action is answered
With being to solve one of effective method of this problem.At present using oriental wormwood parthenium extract, epoxiconazole as active ingredient composition
Not yet there is relevant report applied to the research for preventing and treating false smut.
The content of the invention
It is good the technical problem to be solved in the present invention is to provide a kind of preventive effect, chemical pesticide usage amount can be reduced, delays disease
The opportunistic pathogen resistance to the action of a drug is produced, the bactericidal composition of parthenium extract containing oriental wormwood and epoxiconazole to crop safety, for preventing and treating paddy rice rice
Bent disease.
The technical scheme that present invention solution above-mentioned technical problem is taken is as follows:A kind of parthenium extract containing oriental wormwood and epoxiconazole
Bactericidal composition, active ingredient is made up of oriental wormwood parthenium extract and epoxiconazole, the weight ratio of oriental wormwood parthenium extract and epoxiconazole
For 20-1:1-20.Preferably, the weight ratio of oriental wormwood parthenium extract and epoxiconazole is 10-1:1-10.
Described bactericidal composition active ingredient accounts for the 1-70% of bactericidal composition gross weight, and surplus is to be subjected on agricultural chemicals
Auxiliary element.
Described bactericidal composition adds corresponding auxiliary element and is prepared into according to method known in those skilled in the art
Wettable powder, water dispersible granules or suspending agent.
The auxiliary element that bactericidal composition of the present invention is used include dispersant, wetting agent, disintegrant, binding agent, stabilizer,
Antifreeze, defoamer, thickener, solvent, filler or it is other be beneficial to active ingredient in the formulation stablize and drug effect play
Know material, be all the various composition commonly used in field of pesticide preparation or allow to use.
The preparation method of the oriental wormwood parthenium extract:The tender seedling of Artemisia capillaris is cleaned, dries in the shade, is crushed with pulverizer, 30-60 is crossed
Mesh sieve;The Artemisia capillaris crushed is used into conventional Supercritical carbon dioxide extraction method, extraction conditions are:Extraction temperature is 40-50
DEG C, extracting pressure is 20-40MPa, and carbon dioxide flow is 40-60L/h, and extraction time is 1-2h, that is, Artemisia capillaris is made and extracts
Thing.
Beneficial effect of the present invention:The present invention is compounded by oriental wormwood parthenium extract and epoxiconazole, for rice green smut
Preventing and treating, preventive effect is significantly higher than the preventive effect of single dose.Bactericidal composition of the present invention has synergistic function substantially, and the lasting period is long,
Chemical pesticide usage amount can be reduced, delays the pathogen resistance to the action of a drug to produce, the features such as to crop safety.
Embodiment
Technical scheme and technique effect are described further below in conjunction with specific embodiment, institute of the present invention
The percentage stated is weight percentage.
First, the preparation of oriental wormwood parthenium extract
The preparation method of the oriental wormwood parthenium extract:The tender seedling of Artemisia capillaris is cleaned, dries in the shade, is crushed with pulverizer, 40 mesh are crossed
Sieve;The Artemisia capillaris crushed is used into conventional Supercritical carbon dioxide extraction method, extraction conditions are:Extraction temperature is 40 DEG C, extraction
Pressure power is 40MPa, and carbon dioxide flow is 50L/h, and extraction time is 1.5h, that is, oriental wormwood parthenium extract is made.
2nd, oriental wormwood parthenium extract and epoxiconazole compounding are to rice green smut Toxicity Determination
Subjects:The green pyrenomycetes of Ustilaginoidea virens rice (Ustilaginoidea oryzae).
After determining the effective inhibition concentration scope of each medicament through prerun, each medicament is set 5 agent by active constituent content respectively
Gradient processing is measured, if clear water is compareed.Reference《Farm-chemical indoor determination test rule bactericide》Carry out, using mycelial growth speed
Rate method determines virulence of the medicament to Ustilaginoidea virens.Colony diameter is measured with crossing method after 72h, each processing is calculated net raw
Long amount, mycelial growth inhibition rate.
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into logarithm value (x), with minimum
Square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC of every kind of medicament50Value.Simultaneously according to the abundant methods of Sun Yun
Co-toxicity coefficient method (CTC) evaluate the synergistic effect of pharmacy mix, i.e. CTC≤80, composition shows as antagonism, when 80
<CTC<120, composition shows as summation action, and when CTC >=120, composition shows as synergistic effect.Result of the test such as table 1.
The toxicity test analysis of results table of the oriental wormwood parthenium extract of table 1, epoxiconazole and its compounding to rice green smut
As shown in Table 1, the weight ratio of oriental wormwood parthenium extract and epoxiconazole is in 20-1:Compounded in the range of 1-20 bent to paddy rice rice
Disease shows obvious synergistic effect.Wherein, when oriental wormwood parthenium extract and epoxiconazole weight ratio are in 10-1:In the range of 1-10,
Synergistic effect is especially prominent;When the weight ratio of oriental wormwood parthenium extract and epoxiconazole is 1:When 25, composition shows as summation action;
When the weight ratio of oriental wormwood parthenium extract and epoxiconazole is 25:1、1:When 30, composition shows as antagonism.
3rd, the implementation of Formulation Example
Embodiment 1:30% oriental wormwood parthenium extract epoxiconazole wettable powder (1:1)
Weigh oriental wormwood parthenium extract (active ingredient) 15%, epoxiconazole (active ingredient) 15%, lignosulfonates (scattered
Agent) 5%, lauryl sodium sulfate (wetting agent) 4%, white carbon (filler) 10%, kaolin (filler) complement to 100%;Will
Each component is well mixed in mixed cylinder, is remixed after being crushed through airslide disintegrating mill uniform, you can 30% Artemisia capillaris is made and extracts
Thing epoxiconazole wettable powder.
Embodiment 2:21% oriental wormwood parthenium extract epoxiconazole wettable powder (1:20)
Oriental wormwood parthenium extract (active ingredient) 1%, epoxiconazole (active ingredient) 20%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt
(dispersant) 4%, pull open powder (wetting agent) 3.5%, bentonite (filler) complement to 100%;Each component is mixed in mixed cylinder
Close uniform, remixed after being crushed through airslide disintegrating mill uniform, you can 21% oriental wormwood parthenium extract epoxiconazole wettable powder is made
Agent.Embodiment 3:48% oriental wormwood parthenium extract epoxiconazole water dispersant (15:1)
Oriental wormwood parthenium extract (active ingredient) 45%, epoxiconazole (active ingredient) 3%, polycarboxylate (dispersant) 3%, alkane
Base benzene sulfonic acid calcium salt (dispersant) 3%, spaonin powder (wetting agent) 6%, starch (binding agent) 2.5%, sodium carbonate (disintegrant)
2.5%th, diatomite (filler) complements to 100%;The particle diameter that each component is needed through air-flow crushing together, obtains granulation and uses
Material, item is quantified in feeding fluidized bed granulating dryer after granulation and drying, i.e., obtained 48% oriental wormwood parthenium extract
Epoxiconazole water dispersant.
Embodiment 4:60% oriental wormwood parthenium extract epoxiconazole water dispersant (5:1)
Oriental wormwood parthenium extract (active ingredient) 50%, epoxiconazole (active ingredient) 10%, APES are (scattered
Agent) 7%, it is calcium dodecyl benzene sulfonate (wetting agent) 6.5%, polyvinyl alcohol (binding agent) 3%, ammonium carbonate (disintegrant) 3%, white
Carbon black (filler) 8%, attapulgite (filler) complement to 100%;The particle diameter that each component is needed through air-flow crushing together,
Obtain materials of pelletizing, item is quantified in feeding fluidized bed granulating dryer after granulation and drying, i.e., obtained 60% oriental wormwood
Parthenium extract epoxiconazole water dispersant.
Embodiment 5:22% oriental wormwood parthenium extract epoxiconazole suspension concentrate (10:1)
Oriental wormwood parthenium extract (active ingredient) 20%, epoxiconazole (active ingredient) 2%, AEO are (scattered
Agent) 4.5%, it is lauryl sodium sulfate (wetting agent) 4%, Arabic gum (thickener) 2%, propane diols (antifreezing agent) 2%, organic
Silicone oil (defoamer) 0.4%, deionized water (solvent) complement to 100%;First other auxiliary agents are mixed, sheared in cutter mixed
Close uniform, then add oriental wormwood parthenium extract and epoxiconazole, 3h is ground in ball crusher, you can 22% Artemisia capillaris is made and extracts
Thing epoxiconazole suspension concentrate.
Embodiment 6:11% oriental wormwood parthenium extract epoxiconazole suspension concentrate (1:10)
Oriental wormwood parthenium extract (active ingredient) 1%, epoxiconazole (active ingredient) 10%, APES phosphate
(dispersant) 4%, calcium dodecyl benzene sulfonate (wetting agent) 3.5%, xanthans (thickener) 2%, glycerine (antifreezing agent)
2%th, the aliphatic alcohols (defoamer) 0.1% of C8~10, deionized water (solvent) complement to 100%;First other auxiliary agents are mixed,
Shear-mixed is uniform in cutter, then adds oriental wormwood parthenium extract and epoxiconazole, 3h is ground in ball crusher, you can be made
11% oriental wormwood parthenium extract epoxiconazole suspension concentrate.
Comparative example 1:30% oriental wormwood parthenium extract wettable powder
Weigh oriental wormwood parthenium extract (active ingredient) 30%, lignosulfonates (dispersant) 5%, lauryl sodium sulfate
(wetting agent) 4%, white carbon (filler) 10%, kaolin (filler) complement to 100%;Each component is mixed in mixed cylinder
It is even, remixed after being crushed through airslide disintegrating mill uniform, you can 30% oriental wormwood parthenium extract wettable powder is made.
Comparative example 2:125 g/l of epoxiconazole suspension concentrates, registration card number:PD20151919, BASF plant protection (Jiangsu)
Co., Ltd, it is purchased in market.
4th, the implementation of Formulation Example is applied to preventing and treating rice green smut field control effectiveness test
Test method:This test arrangement is fallen ill heavier throughout the year in Guangxi Binyang County, field false smut.Separately clear water is set as blank
Control, experiment sets 9 processing altogether, often handles 4 repetitions, per plot area 66.7m2, 36 cells, random district's groups row are set altogether
Make ridge isolation between row, cell, prevent agricultural chemicals from interacting.Using spray pesticide, spraying is uniform thoughtful, before the paddy rice cut phase
Spray for the first time within 7 days, in second of spray of paddy rice full heading time, common dispenser is twice.
Investigation method:5 days investigation each cells in field, the point sampling of diagonal 5, every point are used per cell before rice harvesting
The connected 10 clumps of rice strain of investigation, totally 50 clumps, investigates total spike number, sick spike number and sick series, the classification in units of sick fringe calculates the state of an illness and referred to
Number and prevention effect.Water content in harvest, carries out cell production measure.
Disease scale standard:
0 grade:Complete stool is disease-free;
1 grade:Per Cong Pingjun 0.1-1.0 infected seeds;
3 grades:Per Cong Pingjun 1.1-2.0 infected seeds;
5 grades:Per Cong Pingjun 2.1-3.0 infected seeds;
7 grades:Per Cong Pingjun 3.1-4.0 infected seeds;
9 grades:Per more than 4 infected seeds of Cong Pingjun;
Disease index and prevention effect computational methods:
Disease index calculation formula:In formula:X- disease indexs;Ni- sick spike number at different levels;I- phases
To value of series;N- investigates total spike number.
Prevention effect formula:P=(CK-PT)/CK × 100;In formula:P- prevention effects, unit is percentage (%);CK-
Disease index after blank district dispenser;Disease index after PT- chemicals treatment district dispensers.
The oriental wormwood parthenium extract of table 2, epoxiconazole and its compounding preventing and treating rice green smut field control effectiveness test
As can be seen from Table 2, the sterilization synergistic composition that the present invention is compounded with oriental wormwood parthenium extract and epoxiconazole can be effective
Rice green smut is prevented and treated, prevention effect is superior to single dose.Both compoundings can delay the drug-fast generation of germ simultaneously, improve preventing and treating
Effect.And it is equal without obvious harmful effect to trial crops, security is good, meets the security requirement of pesticidal preparations.
Claims (5)
1. the bactericidal composition of a kind of parthenium extract containing oriental wormwood and epoxiconazole, it is characterised in that active ingredient is extracted by Artemisia capillaris
The weight ratio of thing and epoxiconazole composition, oriental wormwood parthenium extract and epoxiconazole is 20-1:1-20.
2. bactericidal composition according to claim 1, it is characterised in that the weight ratio of oriental wormwood parthenium extract and epoxiconazole is
10-1:1-10。
3. bactericidal composition according to claim 1 or 2, it is characterised in that it is total that the active ingredient accounts for bactericidal composition
The 1-70% of weight, surplus is acceptable auxiliary element on agricultural chemicals.
4. bactericidal composition according to claim 3, it is characterised in that described bactericidal composition is prepared into wettable powder
Agent, water dispersible granules or suspending agent.
5. the bactericidal composition described in claim 1 or 2 is used to prevent and treat rice green smut.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101884328A (en) * | 2010-07-07 | 2010-11-17 | 江苏省绿盾植保农药实验有限公司 | Epoxiconazole and validamycin-containing bactericide |
CN102150658A (en) * | 2010-12-06 | 2011-08-17 | 北京颖新泰康国际贸易有限公司 | Bactericide composition and preparation and application thereof |
-
2017
- 2017-06-22 CN CN201710481523.6A patent/CN107212024A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101884328A (en) * | 2010-07-07 | 2010-11-17 | 江苏省绿盾植保农药实验有限公司 | Epoxiconazole and validamycin-containing bactericide |
CN102150658A (en) * | 2010-12-06 | 2011-08-17 | 北京颖新泰康国际贸易有限公司 | Bactericide composition and preparation and application thereof |
Non-Patent Citations (2)
Title |
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薛广波: "《现代消毒学》", 31 July 2002, 人民军医出版社 * |
谢其洲: "50%氟环唑悬浮剂防治稻曲病效果试验初报", 《安徽农学通报》 * |
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