CN107173395A - A kind of plant source bactericidal composition - Google Patents
A kind of plant source bactericidal composition Download PDFInfo
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- CN107173395A CN107173395A CN201710365510.2A CN201710365510A CN107173395A CN 107173395 A CN107173395 A CN 107173395A CN 201710365510 A CN201710365510 A CN 201710365510A CN 107173395 A CN107173395 A CN 107173395A
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- Prior art keywords
- matrine
- bactericidal composition
- plant source
- jia betel
- source bactericidal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of plant source bactericidal composition, the weight ratio that active ingredient constitutes , Jia Betel extracts and matrine You Jia Betel extracts and matrine is 20 1:1‑10.The plant source bactericidal composition that Yi Jia Betel extracts and matrine of the present invention are compounded is used to prevent and treat anthracnose of crop, and obvious synergy reduces cost accounting.The bactericidal composition of the present invention, which has, delays the germ resistance to the action of a drug to produce, pollution-free, the advantages of noresidue, meets agricultural sustainable development direction.And raw material are cheap and easily-available, there is preferable market application foreground.
Description
Technical field
The present invention relates to pesticides application technical field, and in particular to a kind of plant source bactericidal composition, for preventing and treating cucumber
Anthracnose.
Background technology
Anthracnose is the important disease in fruit tree, vegetables production, occurs seriously, to cause plant withered or fruit when condition is suitable
It is real to rot.In China, due to fruit tree, vegetables annually cultivating, cause the bacterium source of anthrax bacteria constantly to accumulate, there is that aggravates year by year to become
Gesture, constitutes serious threat to its normal production.The preventing and treating of anthracnose relies primarily on benzo miaow in relevant fruits and vegetables production at present
The medicament such as azole medicament carbendazim and thiophanate methyl, but the long-term use of single medicament is easy to make germ develop immunity to drugs.
Therefore, the preventing and treating that the active ingredient for screening the different mechanisms of action is compounded to anthracnose is significant.
Matrine (Matrine) is to be extracted by the dry root of legume kuh-seng, plant, fruit through organic solvents such as ethanol
It is made, is a kind of alkaloid, sterling outward appearance is off-white color to white powder.Matrine is a kind of botanical pesticide, with spy
The characteristics of qualitative, natural sex, only specific biology is acted, can be decomposed rapidly in the Nature, final product is dioxy
Change carbon and water；Matrine is the plant endogenous chemical substance active to harmful organism, and composition is not single, but chemical
The not close multigroup combination of the close multigroup and chemical constitution of structure, complements each other, plays a role jointly；Matrine is because more
Chemical substance collective effect is planted, it is not easily caused nuisance and develops immunity to drugs, can be used for a long time.Current country's kuh-seng alkali preparation
There are 0.3% aqueous matrine solution, 0.8% matrine lactone aqua, 1% kuh-seng aqueous slkali, 1.1% kuh-seng aqueous slkali, 1.1% kuh-seng
Ground caustic agent etc., it is good that these medicine based on herbs have been supplied in crop some insects acquirement such as preventing and treating vegetables, fruit tree, tealeaves, tobacco
Preventive effect.Also there is good preventive effect to vegetables downy mildew, epidemic disease, anthracnose in addition, merit attention exploitation.
Jia Betel (scientific names：Piper sarmentosum Roxb.), also known as clam beach wormwood, false beach wormwood, mountain beach wormwood etc., it is Piperaceae pepper
Platymiscium.False Betel originates in Chinese Guangdong, Guangxi, Fujian, Yunnan, Guizhou and Tibet (Motuo) each provinces and regions, is born in by sylvan life or village
On wetland.Jia Betel are pungent, warm-natured, nontoxic.It is pharmaceutically acceptable, radical cure treating rheumatic ostealgia, traumatic injury, cough due to wind-cold evil, gestation and postpartum water
Swell；Also edible, people are usually cooked with its leaf, and its delicious food is often mentioned in the same breath with purple perilla.False Betel stems, Ye Shui
Immersion liquid has inhibitory action to staphylococcus aureus, shigella flexneri etc.., Jia Betel also have antipyretic-antalgic, anti-inflammatory, drop simultaneously
The effect such as bone healing after hypoglycemia, anti-oxidant and promotion fracture.The report acted at present on Jia Betel extracts killing pests and suppressing bacteria
Road is less, and Chinese Patent Application No. 2009102358119 discloses a kind of Jia Betel extracts and its in killing pests and suppressing bacteria agent is prepared
Application, Jiang Jia Betel extracts be used for prepare pesticide-germicide microemulsion, it is to prodenia litura, roadway caving mining, radish aphid, bajiao banana
Anthrax bacteria and rubber anthrax bacteria have good prevention effect.At present using Jia Betel as raw material, it is applied to matrine compound
The research of preventing and treating fruit and vegetable anthracnose not yet has relevant report.
The content of the invention
It is good the technical problem to be solved in the present invention is to provide a kind of prevention effect, the germ resistance to the action of a drug can be delayed to produce, no dirt
Dye, the plant source bactericidal composition of noresidue, applied to preventing and treating cucumber anthracnose.
The technical scheme that present invention solution above-mentioned technical problem is taken is as follows：A kind of plant source bactericidal composition, effectively
The weight ratio that composition constitutes , Jia Betel extracts and matrine You Jia Betel extracts and matrine is 20-1:1-10.Preferably, it is false
The weight ratio of Betel extracts and matrine is 5:1、1:1.
Described its active ingredient of plant source bactericidal composition accounts for the 1-50% of plant source bactericidal composition gross weight, surplus
For acceptable auxiliary element on agricultural chemicals.
Described bactericidal composition adds corresponding auxiliary element, auxiliary agent according to method known in those skilled in the art
The formulation being made is wettable powder or aqueous emulsion.
Plant source bactericidal composition of the present invention is subjected to auxiliary element, auxiliary agent for dispersant, wetting agent, emulsification
The conventional functionality auxiliary agent such as agent, antifreezing agent, thickener, stabilizer, solvent, filler, is commonly used or fair in field of pesticide preparation
Perhaps the various composition used, is not particularly limited, and specific composition and consumption are determined according to recipe requirements by simple experiment.
The dispersant, wetting agent and emulsifying agent include sodium metnylene bis-naphthalene sulfonate, the formaldehyde condensation of methyl naphthalene sulfonic acid sodium
Thing, sodium lignin sulfonate, sodium carboxymethylcellulose, polycarboxylate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, Negel, first
Formaldehyde condensation products, sldium lauryl sulfate, calcium dodecyl benzene sulfonate, alkyl benzene sulfonate calcium salt, the dodecyl of base sodium naphthalene sulfonate
Sodium sulphate, pull open powder, APES, aliphatic alcohol polyoxyvinethene phosphate, AEO, dibutyl naphthalene
Sodium sulfonate, NPE, styrylphenol polyoxyethylene ether, Nongru-700 #, agriculture breast 2201#, Span-60#, breast
Agent T-60, TX-10, agriculture breast 1601#, agriculture breast 600#, agriculture breast 400#.
The stabilizer includes sodium citrate, resorcinol.
The thickener includes xanthans, Arabic gum, Magnesiumaluminumsilicate.
The antifreezing agent includes ethylene glycol, propane diols, urea, ammonium sulfate.
The filler includes kaolin, attapulgite, white carbon, soil powder, diatomite, bentonite.
The solvent includes deionized water, toluene, dimethylbenzene, ethanol.
The Jia Betel extracts are prepared from by the following method：Jia Betel complete stools are cleaned, dries in the shade, is crushed with pulverizer, mistake
40-50 mesh sieves, obtain crushed material；5-10 times is added in gained crushed material to measure after 95% ethanol, soaking at room temperature 24h, ultrasonic extraction
30-40min, with filtered on buchner funnel, collects filtrate.Extracted 2-3 times with method, merge all filtrates, then use Rotary Evaporators
It is concentrated under reduced pressure into relative density and is 1.10-1.20 paste, then be freeze-dried into powder, that is, Jia Betel extracts is made.
Beneficial effect of the present invention：The plant source bactericidal composition that Yi Jia Betel extracts and matrine of the present invention are compounded is used
In preventing and treating cucumber anthracnose, obvious synergy reduces cost accounting.The bactericidal composition of the present invention, which has, delays germ
The resistance to the action of a drug is produced, pollution-free, the advantages of noresidue, meets agricultural sustainable development direction.And raw material are cheap and easily-available, have preferably
Market application foreground.
Embodiment
Technical scheme and technique effect are described further below in conjunction with specific embodiment, institute of the present invention
The percentage stated is weight percentage.
First, the preparation of Jia Betel extracts
The Jia Betel extracts are prepared from by the following method：Jia Betel complete stools are cleaned, dries in the shade, is crushed with pulverizer, mistake
40 mesh sieves, obtain crushed material；Added in gained crushed material after 8 times of 95% ethanol of amount, soaking at room temperature 24h, in 45 DEG C, 100Hz bars
Ultrasonic extraction 40min under part, with filtered on buchner funnel, collects filtrate.Extracted 3 times with method, merge all filtrates, then with rotation
Evaporation under reduced pressure is concentrated into relative density and is 1.15 paste, then is freeze-dried into powder, that is, Jia Betel extracts are made.
2nd, Toxicity Determination
Experimental subjects：Cucumber anthracnose (Colletotrichum orbiculare).
Test method：With reference to《People's Republic of China (PRC) agricultural industry criteria NY/T 1156.2-2006》Mycelial growth rate
Method.After determining the effective inhibition concentration scope of each medicament through prerun, each medicament is set at 5 dosage respectively by active constituent content
Reason, if clear water is compareed.By pathogen PDA culture medium culture, when bacterium colony just covers with culture dish, with diameter 5mm card punch
Fungus block is broken into colony edge, fungus block is moved to the toxic PDA culture medium center being configured in advance with transfer needle, 25 are subsequently placed in
Cultivated in DEG C incubator, often processing is repeated 4 times.Each processing colony diameter is measured using crossing method after 72h, each processing is calculated
Net growth, mycelial growth inhibition rate.
Mycelial growth inhibition rate is converted into probit value (y), liquor strength (μ g/mL) is converted into logarithm value (x), with minimum
Square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC of every kind of medicament50Value.Simultaneously according to the abundant methods of Sun Yun
Co-toxicity coefficient method (CTC) evaluate the synergistic effect of pharmacy mix, i.e. CTC≤80, composition shows as antagonism, when 80
<CTC<120, composition shows as summation action, and when CTC >=120, composition shows as synergistic effect.Result of the test such as table 1.
The toxicity test analysis of results table of the Jia Betel extracts of table 1, matrine and its compounding to cucumber anthracnose
The weight ratio of , Jia Betel extracts and matrine is in 20-1 as shown in Table 1:Mixture is to cucumber anthracnose in the range of 1-10
Bacterium shows obvious synergistic effect.Wherein, Dang Jia Betel extracts and matrine weight ratio are 5:1、1:Synergistic effect shows when 1
Write；Dang Jia Betel extracts are 25 with matrine weight ratio:1、1:When 15, it shows as summation action；Dang Jia Betel extracts and hardship
It is 1 to join alkali weight ratio:When 18, it shows as antagonism.
3rd, product the implementation of Formulation Example
Embodiment 1：6% Jia Betel extracts aqueous matrine solution (5:1)
It is Jia Betel extracts (active ingredient) 5%, matrine (active ingredient) 1%, polycarboxylate (dispersant) 2%, wooden
Plain sodium sulfonate (dispersant) 1.5%, lauryl sodium sulfate (wetting agent) 3%, kaolin (filler) complement to 100%；Will be each
Component is well mixed, and 6% Jia Betel extract aqueous matrine solutions are made after being pulverized and mixed in size-reduced machine.
Embodiment 2：5.5% Jia Betel extracts aqueous matrine solution (10:1)
Jia Betel extracts (active ingredient) 5%, matrine (active ingredient) 0.5%, the formaldehyde condensation of methyl naphthalene sulfonic acid sodium
Thing (dispersant) 3.5%, calcium dodecyl benzene sulfonate (wetting agent) 3%, diatomite (filler) complement to 100%；By each component
Well mixed, 5.5% Jia Betel extract aqueous matrine solutions are made after being pulverized and mixed in size-reduced machine.
Embodiment 3：5% Jia Betel extract matrine waters emulsion (1:1)
Jia Betel extracts (active ingredient) 2.5%, matrine (active ingredient) 2.5%, sodium lignin sulfonate (dispersant)
3%th, NPE (emulsifying agent) 4%, sodium citrate (stabilizer) 1%, dimethylbenzene (solvent) 10%, mixed dissolution
Obtain oil phase；Ethylene glycol (antifreezing agent) 1%, xanthans (thickener) 0.2%, deionized water (solvent) complement to 100%, are made into
Aqueous phase.Oil phase adduction, which enters in aqueous phase, to be sufficiently mixed, after high-shear emulsifying, that is, 5% vacation Betel extract matrine water and milk is made
Agent.
Embodiment 4：3% Jia Betel extract matrine waters emulsion (1:5)
Jia Betel extracts (active ingredient) 0.5%, matrine (active ingredient) 2.5%, alkyl benzene sulfonate calcium salt are (scattered
Agent) 3%, aliphatic alcohol polyoxyvinethene phosphate (emulsifying agent) 3%, resorcinol (stabilizer) 1%, toluene (solvent) 8%, mix
Close dissolving and obtain oil phase；Propane diols (antifreezing agent) 1%, Arabic gum (thickener) 0.1%, deionized water (solvent) are complemented to
100%, it is made into aqueous phase.Oil phase adduction, which enters in aqueous phase, to be sufficiently mixed, after high-shear emulsifying, that is, 3% Jia Betel extracts are made
Matrine water emulsion.
Embodiment 5：8% Jia Betel extract matrine waters emulsion (15:1)
Jia Betel extracts (active ingredient) 7.5%, matrine (active ingredient) 0.5%, polycarboxylate (dispersant)
3.5%th, styrylphenol polyoxyethylene ether (emulsifying agent) 2.5%, agriculture breast 600# (emulsifying agent) 2%, sodium citrate are (stable
Agent) 1%, dimethylbenzene (solvent) 15%, mixed dissolution obtains oil phase；Ethylene glycol (antifreezing agent) 1%, Magnesiumaluminumsilicate (thickener)
0.2%th, deionized water (solvent) complements to 100%, is made into aqueous phase.Oil phase adduction, which enters in aqueous phase, to be sufficiently mixed, through high shear breast
After change, that is, 8% Jia Betel extract matrine water emulsions are made.
Comparative example 1：5% Jia Betel extract wettable powders
Jia Betel extracts (active ingredient) 5%, polycarboxylate (dispersant) 2%, sodium lignin sulfonate (dispersant)
1.5%th, lauryl sodium sulfate (wetting agent) 3%, kaolin (filler) complement to 100%；Each component is well mixed, through powder
5% Jia Betel extract wettable powders are made after being pulverized and mixed in broken machine.
Comparative example 2：0.3% aqueous matrine solution, registration card number：PD20150393, Shaanxi Kang He found rich biotechnology medicine company
Co., Ltd, it is purchased in market.
4th, the implementation of Formulation Example is applied to preventing and treating cucumber anthracnose field control effectiveness test
Test method：This experimental arrangement is in Nanning suburbs.Experiment sets 7 processing altogether, often handles 4 repetitions, per small
Area area 40m2, totally 28 cells, random district's groups arrangement.Using Jacto-40 type knapsack hand sprayer spray pesticides, spray
Mist is uniformly thoughtful, using clear water as blank control, is 675L/hm2 with amount of liquid medicine.First time dispenser at the initial stage of a disease, second after 7 days
Secondary dispenser, second of dispenser carries out third time dispenser after 7 days, altogether dispenser three times.
Investigation method：Before dispenser, 7 days after third time dispenser, each cell is investigated within 14 days, per the 5 points of samplings of cell diagonal,
Every is investigated 3 plants, and the whole blades of every plant of investigation, the percentage for accounting for whole leaf area by scab on blade is classified, record total number of sheets,
The sick numbers of sheets at different levels and sick series, calculate disease index and prevent and treat preventive effect.
Grade scale：
0 grade：Disease-free spot；
1 grade：Scab accounts for less than the 5% of whole leaf area；
3 grades：Scab accounts for the 6-10% of whole leaf area；
5 grades：Scab accounts for the 11-25% of whole leaf area；
7 grades：Scab accounts for the 26-50% of whole leaf area；
9 grades：Scab accounts for more than the 50% of whole leaf area.
Disease index and prevention effect computational methods：
Disease index calculation formula：
In formula：
X --- disease index；
Ni--- the sick numbers of sheets at different levels；
I --- with respect to value of series；
N --- investigation total number of sheets.
Prevention effect formula：
In formula：
P --- prevention effect, unit is percentage (%)；
CK0--- disease index before blank district dispenser；
CK1--- disease index after blank district dispenser；
PT0--- disease index before chemicals treatment district dispenser；
PT1--- disease index after chemicals treatment district dispenser.
The Jia Betel extracts of table 2, matrine and its compounding preventing and treating cucumber anthracnose test of pesticide effectiveness
The plant source bactericidal composition of , Jia Betel extracts and matrine compound can effectively prevent and treat cucumber as can be seen from Table 2
Anthracnose, prevention effect is significantly higher than the preventive effect of alone Jia Betel extracts and matrine.Both mixtures can delay germ to resist simultaneously
The generation of the property of medicine, improves prevention effect.Plant source bactericidal composition of the present invention is equal to trial crops without obvious harmful effect, safety
Property is good, meets the security requirement of pesticidal preparations.
Claims (5)
1. a kind of plant source bactericidal composition, it is characterised in that active ingredient constitutes , Jia Betel You Jia Betel extracts and matrine and carried
The weight ratio for taking thing and matrine is 20-1:1-10.
2. plant source bactericidal composition according to claim 1, it is characterised in that the weight of Jia Betel extracts and matrine
Than for 5:1、1:1.
3. plant source bactericidal composition according to claim 1 or 2, it is characterised in that described active ingredient accounts for plant
The 1-50% of source bactericidal composition gross weight, surplus is acceptable auxiliary element on agricultural chemicals.
4. plant source bactericidal composition according to claim 3, it is characterised in that described plant source bactericidal composition system
It is standby into wettable powder or aqueous emulsion.
5. plant source bactericidal composition according to claim 1, it is characterised in that described plant source bactericidal composition is used
In preventing and treating cucumber anthracnose.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108271812A (en) * | 2017-12-29 | 2018-07-13 | 广西南宁乐蕊生物科技有限责任公司 | A kind of Synergistic bactericidal composition and its application |
-
2017
- 2017-05-22 CN CN201710365510.2A patent/CN107173395A/en active Pending
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Application publication date: 20170919 |