CN107211927A - A kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus - Google Patents
A kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus, seed selection step is：Build up lefteye flounder family, determine half lethal concentration, the disease-resistant second generation of seed selection lefteye flounder, the disease-resistant third generation of seed selection lefteye flounder.Have the beneficial effect that：Simple to operate, without highly difficult operation, reproducibility is strong, alleviates the workload of breeding；The bioactive peptide that injection amino acid sequence is SCASRCKSRRCRARRCGYFGICKCFRC in metainfective lefteye flounder water is cultivated, above-mentioned bioactive peptide will not can be directly reached small intestine, be digested and assimilated by small intestine by pepsin and trypsin digestion in lefteye flounder body；The formation speed of leucocyte and macrophage in lefteye flounder body can be significantly improved, the nospecific immunity of lefteye flounder is improved；The obtained disease-resistant third generation of lefteye flounder is significant and application value to the resistance of Vibrio anguillarum, breeding survival rate raising 30 ~ 55% to China's lefteye flounder cultivation.
Description
Technical field
Field is cultivated the present invention relates to the seed selection of lefteye flounder fish, and in particular to a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus.
Background technology
Selection and use is also known as line breeding, is a kind of most basic means of fish breeding work.Selection and use is more with growth
The economic characters such as rate, cultivation survival rate, premunition, resistance and qualitative character target are selected, and are the bases of fish breeding
Research method.System of selection mainly includes colony's selection, mixing selection, families selecting and comprehensive selection etc..This method simplicity,
It is effective, it is suitable for the strong animal of the such reproductive capacity of fish, so being the method commonly used during fish are cultivated.Lefteye flounder is cold temperature
Property, bottom is dwelt type seawater fish, is the important sea-farming economic fish of the countries such as China, Japan and South Korea.
But, long-term inbred and high-density breeding causes lefteye flounder germplasm to be degenerated, and grows slack-off, premunition decline,
Disease is caused to take place frequently, yield declines, and huge economic losses are brought to lefteye flounder aquaculture industry.Traditional fish disease treatment method mainly by
Using antibiotic, and a large amount of use of antibiotic can not only produce the problems such as medicament residue, drug resistance and environmental pollution, and right
There is also potential influence for the health of consumer.Vibrio anguillarum is one kind that maximum is endangered in lefteye flounder bacterial disease, lefteye flounder cultivating process
In, because of infection Vibrio anguillarum, the survival rate reduction of lefteye flounder brings larger economic loss to China's lefteye flounder cultivation.
Prior art such as Authorization Notice No. is CN101911918B Chinese invention patent, discloses a kind of lefteye flounder disease-resistant excellent
Non-defective unit system selective breeding method, including lefteye flounder unexpected mass incident；Lefteye flounder family selective breeding；Also the molecular labeling including the disease-resistant family of lefteye flounder is aided in
Seed selection；Or include the artificial gynogenesis seed selection of the disease-resistant family of lefteye flounder；Or include the inbreeding seed selection of the disease-resistant family of lefteye flounder.With lefteye flounder
Establish the disease-resistant excellent strain selection of China's seawater fish first for material, cultivate what anti-Eel vibriosises power was significantly improved
Lefteye flounder excellent strain, breeding survival rate improves 20 ~ 47%, and growth rate improves more than 20%.This method is selected for fish disease-resistant variety
Educate and open new technological approaches, popularization and application are suitably carried out in all cultured fishes, to the disease-resistant prevalent variety cultivation of cultured fishes
Significant and application value.But this method is cumbersome, workload is huge, and the breeding survival rate of lefteye flounder need to be carried
It is high.
The content of the invention
It is an object of the invention to provide one kind it is simple to operate, workload is less, the good tooth of obtained lefteye flounder disease-resistant performance
The disease-resistant excellent strain selection of flounder.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of disease-resistant superior strains of paralichthys olivaceus
Selection, including build up lefteye flounder family, determine half lethal concentration, the disease-resistant second generation of seed selection lefteye flounder, seed selection lefteye flounder the disease-resistant 3rd
Generation.The obtained disease-resistant third generation of lefteye flounder is to the resistance of Vibrio anguillarum, breeding survival rate raising 30 ~ 55%.
Build up lefteye flounder family step to collect the lefteye flounder fry from country variant or marine site, select to advise in each geographical population
Lattice are neat, the fry of physique healthy and strong is cultivated and screened, and is selection intensity with 5% when fry growth is to 10 ~ 13 centimetres,
Individual 150 ~ 250 big tails are selected from 3000 ~ 5000 tail fries and carry out individually cultivation up to maturation, and are used as the disease-resistant choosing of lefteye flounder
Educate the first generation；Family is set up by intraspecific hybridization：By the raun of the milter or raun of disease-resistance population respectively with Different population
Or milter carries out pairing breeding and sets up family, or a geographical population and the hybridization of another geographical population are set up into family；In fish
When seedling closes on incubation of membrane, it is transferred into nursery cylinder and is cultivated, a family is cultivated per cylinder, and per cubic meter of water is trained
Educate 5000 ~ 10000 tail prelarvas.Abundant lefteye flounder parent is obtained with species hybridization by intraspecific hybridization and diverse geographic location,
Seed selection operation for after provides the gene pool of abundance.
It is, when fry rearing is to 3.5 ~ 5.5 monthly age, 150 ~ 250 tails to be chosen from each family to determine half lethal concentration step
Fry carries out Vibrio anguillarum immersion infection：The population mixture of the identical quantity fry from each family is chosen, it is dilute with physiological saline
It is 20 ~ 30OD to release cultured Vibrio anguillarum bacterium solution to concentration, above-mentioned bacterium solution is diluted to 50 with seawater, 100,200,400,
800 times of various concentrations, then 100 ~ 200 tail mixed family fries are placed in the seawater of various concentrations bacterium solution, immersion infection 2 ~ 6
Gone to after hour and observation is cultivated in fresh seawater, the fry death rate of various concentrations group is calculated in subsequent 5 ~ 15 days, will be drawn
The concentration for playing 40 ~ 50% fry death is defined as half lethal concentration.
The disease-resistant second generation step of seed selection lefteye flounder is that lefteye flounder is infected using the Vibrio anguillarum bacterium solution of half lethal concentration, is calculated
The survival rate of each family fry, calculates each according to the survival rate, mating pattern, Parent of family source and family setup time
The odds ratio of family premunition, chooses disease-resistant family fry and is used as the disease-resistant second generation of lefteye flounder.The disease-resistant second generation step of seed selection lefteye flounder
It is middle each to cultivate the infection of each family in the sink with fish, inject bioactive peptide in water, the concentration of bioactive peptide for 0.2 ~
0.6mg/L.The amino acid sequence of bioactive peptide is SCASRCKSRRCRARRCGYFGICKCFRC.Above-mentioned bioactive peptide will not be by
Pepsin and trypsin digestion in lefteye flounder body, can directly reach small intestine, be digested and assimilated by small intestine；Lefteye flounder body can be significantly improved
The formation speed of interior leucocyte and macrophage, improves the nospecific immunity of lefteye flounder.
The disease-resistant third generation step of seed selection lefteye flounder is, by the disease-resistant second generation inbreeding seed selection of lefteye flounder or artificial gynogenesis seed selection, to obtain
To the disease-resistant third generation of lefteye flounder.
Compared with prior art, the advantage of the invention is that：1）Simple to operate, without highly difficult operation, reproducibility is strong, subtracts
The light workload of breeding；2）Injection amino acid sequence is in metainfective lefteye flounder water is cultivated
SCASRCKSRRCRARRCGYFGICKCFRC bioactive peptide, above-mentioned bioactive peptide will not be by pepsin and pancreas in lefteye flounder body
Protease hydrolyzed, can directly reach small intestine, be digested and assimilated by small intestine；Leucocyte and macrophage in lefteye flounder body can be significantly improved
Formation speed, improves the nospecific immunity of lefteye flounder；3）The obtained disease-resistant third generation of lefteye flounder is deposited to the resistance of Vibrio anguillarum, cultivation
Motility rate improves 30 ~ 55%, and China's lefteye flounder cultivation is significant and application value.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus, including build up lefteye flounder family, determine that half lethal concentration, seed selection lefteye flounder are disease-resistant
The second generation, the disease-resistant third generation of seed selection lefteye flounder.The obtained disease-resistant third generation of lefteye flounder is to the resistance of Vibrio anguillarum, breeding survival rate raising
48%。
Build up lefteye flounder family step to collect the lefteye flounder fry from country variant or marine site, select to advise in each geographical population
Lattice are neat, the fry of physique healthy and strong is cultivated and screened, and is selection intensity with 5% when fry growth is to 12 centimetres, from
200 tails of individual greatly are selected in 4000 tail fries and carry out individually cultivation up to maturation, and are used as the disease-resistant seed selection first generation of lefteye flounder；It is logical
Cross intraspecific hybridization and set up family：Raun or milter by the milter or raun of disease-resistance population respectively with Different population are matched somebody with somebody
Family is set up to breeding, or a geographical population and the hybridization of another geographical population are set up into family；Close on and hatch in fry
During film, it is transferred into nursery cylinder and is cultivated, a family is cultivated per cylinder, and per cubic meter of water cultivates 6000 tails son
Fish.Abundant lefteye flounder parent is obtained with species hybridization by intraspecific hybridization and diverse geographic location, the seed selection operation after being
Sufficient gene pool is provided.
It is, when fry rearing is to 4 monthly age, 200 tail fries to be chosen from each family and carry out eel to determine half lethal concentration step
Vibrios immersion infection：The population mixture of the identical quantity fry from each family is chosen, has been cultivated with normal saline dilution
Vibrio anguillarum bacterium solution to concentration be 25OD, above-mentioned bacterium solution is diluted to 50 with seawater, 100,200,400,800 times different dense
Degree, then 150 tail mixed family fries are placed in the seawater of various concentrations bacterium solution, immersion infection goes to fresh seawater after 4 hours
Middle cultivation observation, calculates the fry death rate of various concentrations group in subsequent 10 days, by dead dense of the fry for causing 45%
Degree is defined as half lethal concentration.
The disease-resistant second generation step of seed selection lefteye flounder is that lefteye flounder is infected using the Vibrio anguillarum bacterium solution of half lethal concentration, is calculated
The survival rate of each family fry, calculates each according to the survival rate, mating pattern, Parent of family source and family setup time
The odds ratio of family premunition, chooses disease-resistant family fry and is used as the disease-resistant second generation of lefteye flounder.The disease-resistant second generation step of seed selection lefteye flounder
It is middle each to cultivate the infection of each family in the sink with fish, bioactive peptide is injected in water, the concentration of bioactive peptide is 0.5mg/
L.The amino acid sequence of bioactive peptide is SCASRCKSRRCRARRCGYFGICKCFRC.Above-mentioned bioactive peptide will not be by lefteye flounder body
Interior pepsin and trypsin digestion, can directly reach small intestine, be digested and assimilated by small intestine；It can significantly improve white thin in lefteye flounder body
Born of the same parents and the formation speed of macrophage, improve the nospecific immunity of lefteye flounder.
The disease-resistant third generation step of seed selection lefteye flounder is, by the disease-resistant second generation inbreeding seed selection of lefteye flounder or artificial gynogenesis seed selection, to obtain
To the disease-resistant third generation of lefteye flounder.
Embodiment 2：
A kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus, including build up lefteye flounder family, determine that half lethal concentration, seed selection lefteye flounder are disease-resistant
The second generation, the disease-resistant third generation of seed selection lefteye flounder.The obtained disease-resistant third generation of lefteye flounder is to the resistance of Vibrio anguillarum, breeding survival rate raising
50%。
Build up lefteye flounder family step to collect the lefteye flounder fry from country variant or marine site, select to advise in each geographical population
Lattice are neat, the fry of physique healthy and strong is cultivated and screened, and is selection intensity with 5% when fry growth is to 10 centimetres, from
150 tails of individual greatly are selected in 3000 tail fries and carry out individually cultivation up to maturation, and are used as the disease-resistant seed selection first generation of lefteye flounder；It is logical
Cross intraspecific hybridization and set up family：Raun or milter by the milter or raun of disease-resistance population respectively with Different population are matched somebody with somebody
Family is set up to breeding, or a geographical population and the hybridization of another geographical population are set up into family；Close on and hatch in fry
During film, it is transferred into nursery cylinder and is cultivated, a family is cultivated per cylinder, and per cubic meter of water cultivates 6000 tails son
Fish.Abundant lefteye flounder parent is obtained with species hybridization by intraspecific hybridization and diverse geographic location, the seed selection operation after being
Sufficient gene pool is provided.
It is, when fry rearing is to 4.5 monthly age, the progress of 150 tail fries to be chosen from each family to determine half lethal concentration step
Vibrio anguillarum immersion infection：The population mixture of the identical quantity fry from each family is chosen, has been cultivated with normal saline dilution
Good Vibrio anguillarum bacterium solution to concentration is 26OD, above-mentioned bacterium solution is diluted into 50 with seawater, 100,200,400,800 times different dense
Degree, then 100 tail mixed family fries are placed in the seawater of various concentrations bacterium solution, immersion infection goes to fresh sea after 2 ~ 6 hours
Observation is cultivated in water, the fry death rate of various concentrations group is calculated in subsequent 10 days, the fry for causing 45% is dead
Concentration is defined as half lethal concentration.
The disease-resistant second generation step of seed selection lefteye flounder is that lefteye flounder is infected using the Vibrio anguillarum bacterium solution of half lethal concentration, is calculated
The survival rate of each family fry, calculates each according to the survival rate, mating pattern, Parent of family source and family setup time
The odds ratio of family premunition, chooses disease-resistant family fry and is used as the disease-resistant second generation of lefteye flounder.The disease-resistant second generation step of seed selection lefteye flounder
It is middle each to cultivate the infection of each family in the sink with fish, bioactive peptide is injected in water, the concentration of bioactive peptide is 0.5mg/
L.The amino acid sequence of bioactive peptide is SCASRCKSRRCRARRCGYFGICKCFRC.Above-mentioned bioactive peptide will not be by lefteye flounder body
Interior pepsin and trypsin digestion, can directly reach small intestine, be digested and assimilated by small intestine；It can significantly improve white thin in lefteye flounder body
Born of the same parents and the formation speed of macrophage, improve the nospecific immunity of lefteye flounder.
The disease-resistant third generation step of seed selection lefteye flounder is, by the disease-resistant second generation inbreeding seed selection of lefteye flounder or artificial gynogenesis seed selection, to obtain
To the disease-resistant third generation of lefteye flounder.
Embodiment 3：
A kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus, including build up lefteye flounder family, determine that half lethal concentration, seed selection lefteye flounder are disease-resistant
The second generation, the disease-resistant third generation of seed selection lefteye flounder.The obtained disease-resistant third generation of lefteye flounder is to the resistance of Vibrio anguillarum, breeding survival rate raising
43%。
Build up lefteye flounder family step to collect the lefteye flounder fry from country variant or marine site, select to advise in each geographical population
Lattice are neat, the fry of physique healthy and strong is cultivated and screened, and is selection intensity with 5% when fry growth is to 12 centimetres, from
200 tails of individual greatly are selected in 4000 tail fries and carry out individually cultivation up to maturation, and are used as the disease-resistant seed selection first generation of lefteye flounder；It is logical
Cross intraspecific hybridization and set up family：Raun or milter by the milter or raun of disease-resistance population respectively with Different population are matched somebody with somebody
Family is set up to breeding, or a geographical population and the hybridization of another geographical population are set up into family；Close on and hatch in fry
During film, it is transferred into nursery cylinder and is cultivated, a family is cultivated per cylinder, and per cubic meter of water cultivates 7000 tails son
Fish.Abundant lefteye flounder parent is obtained with species hybridization by intraspecific hybridization and diverse geographic location, the seed selection operation after being
Sufficient gene pool is provided.
It is, when fry rearing is to 4 monthly age, 200 tail fries to be chosen from each family and carry out eel to determine half lethal concentration step
Vibrios immersion infection：The population mixture of the identical quantity fry from each family is chosen, has been cultivated with normal saline dilution
Vibrio anguillarum bacterium solution to concentration be 24OD, above-mentioned bacterium solution is diluted to 50 with seawater, 100,200,400,800 times different dense
Degree, then 120 tail mixed family fries are placed in the seawater of various concentrations bacterium solution, immersion infection goes to fresh seawater after 4 hours
Middle cultivation observation, calculates the fry death rate of various concentrations group in subsequent 10 days, by dead dense of the fry for causing 46%
Degree is defined as half lethal concentration.
The disease-resistant second generation step of seed selection lefteye flounder is that lefteye flounder is infected using the Vibrio anguillarum bacterium solution of half lethal concentration, is calculated
The survival rate of each family fry, calculates each according to the survival rate, mating pattern, Parent of family source and family setup time
The odds ratio of family premunition, chooses disease-resistant family fry and is used as the disease-resistant second generation of lefteye flounder.The disease-resistant second generation step of seed selection lefteye flounder
It is middle each to cultivate the infection of each family in the sink with fish, bioactive peptide is injected in water, the concentration of bioactive peptide is 0.4mg/
L.The amino acid sequence of bioactive peptide is SCASRCKSRRCRARRCGYFGICKCFRC.Above-mentioned bioactive peptide will not be by lefteye flounder body
Interior pepsin and trypsin digestion, can directly reach small intestine, be digested and assimilated by small intestine；It can significantly improve white thin in lefteye flounder body
Born of the same parents and the formation speed of macrophage, improve the nospecific immunity of lefteye flounder.
The disease-resistant third generation step of seed selection lefteye flounder is, by the disease-resistant second generation inbreeding seed selection of lefteye flounder or artificial gynogenesis seed selection, to obtain
To the disease-resistant third generation of lefteye flounder.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Culturing high-quality seedling election effects institute of Zhoushan Putuo Xinghai County
<120>A kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 27
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Arg Cys Arg Ala Arg Arg Cys
1 5 10 15
Gly Tyr Phe Gly Ile Cys Lys Cys Phe Arg Cys
20 25
Claims (6)
1. a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus, including build up lefteye flounder family, determine that half lethal concentration, seed selection lefteye flounder resist
The sick second generation, the disease-resistant third generation of seed selection lefteye flounder, it is characterised in that：The disease-resistant second generation step of described seed selection lefteye flounder is to be caused using half
The Vibrio anguillarum bacterium solution of dead concentration is infected lefteye flounder, calculates the survival rate of each family fry, according to the survival rate of family, mating
Mode, Parent source and family setup time calculate the odds ratio of each family premunition, choose disease-resistant family fry conduct
The disease-resistant second generation of lefteye flounder.
2. a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus according to claim 1, it is characterised in that：Described seed selection tooth
The infection of each family is each cultivated in the sink with fish in the disease-resistant second generation step of flounder, bioactive peptide is injected in water, activity is short
The concentration of peptide is 0.2 ~ 0.6mg/L.
3. a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus according to claim 2, it is characterised in that：Described is active short
The amino acid sequence of peptide is SCASRCKSRRCRARRCGYFGICKCFRC.
4. a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus according to claim 1, it is characterised in that：Described builds up tooth
Flounder family step selects neat specification, constitution in each geographical population to be good for collect the lefteye flounder fry from country variant or marine site
Strong fry is cultivated and screened, and is selection intensity with 5%, from 3000 ~ 5000 tails when fry growth is to 10 ~ 13 centimetres
150 ~ 250 tails of individual greatly are selected in fry and carry out individually cultivation up to maturation, and are used as the disease-resistant seed selection first generation of lefteye flounder；Pass through
Family is set up in intraspecific hybridization：Raun or milter by the milter or raun of disease-resistance population respectively with Different population are matched
Family is set up in breeding, or a geographical population and the hybridization of another geographical population are set up into family；Incubation of membrane is closed in fry
When, it is transferred into nursery cylinder and is cultivated, a family is cultivated per cylinder, and per cubic meter of water cultivates 5000 ~ 10000 tails
Prelarva.
5. a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus according to claim 1, it is characterised in that：Described determination half
Lethasl concentration step is, when fry rearing is to 3.5 ~ 5.5 monthly age, 150 ~ 250 tail fries progress Vibrio anguillarum to be chosen from each family
Immersion infection：The population mixture of the identical quantity fry from each family is chosen, with normal saline dilution cultured eel
Vibrios bacterium solution to concentration is 20 ~ 30OD, above-mentioned bacterium solution is diluted into 50 with seawater, 100,200,400,800 times of various concentrations,
100 ~ 200 tail mixed family fries are placed in the seawater of various concentrations bacterium solution again, immersion infection goes to fresh sea after 2 ~ 6 hours
Observation is cultivated in water, the fry death rate of various concentrations group is calculated in subsequent 5 ~ 15 days, the fry for causing 40 ~ 50% is dead
The concentration died is defined as half lethal concentration.
6. a kind of selective breeding method for disease-resistant superior strains of paralichthys olivaceus according to claim 1, it is characterised in that：Described seed selection tooth
The disease-resistant third generation step of flounder is, by the disease-resistant second generation inbreeding seed selection of lefteye flounder or artificial gynogenesis seed selection, to obtain lefteye flounder the disease-resistant 3rd
Generation.
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Citations (5)
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|---|---|---|---|---|
| CN101810148A (en) * | 2010-04-28 | 2010-08-25 | 中国水产科学研究院黄海水产研究所 | Cross breeding method of fine variety of paralichthysolivaceus |
| CN101911918A (en) * | 2010-03-18 | 2010-12-15 | 中国水产科学研究院黄海水产研究所 | Selective breeding method for disease-resistant superior strains of paralichthys olivaceus |
| CN101699998B (en) * | 2009-11-03 | 2011-10-12 | 中国水产科学研究院黄海水产研究所 | Selection and culture method of excellent breed large-tooth flounder featuring fast growth and high survival rate |
| CN103005164A (en) * | 2012-12-29 | 2013-04-03 | 青岛农业大学 | Compound nucleotide immunoenhancer for paralichthys olivaceus |
| US20140298495A1 (en) * | 2011-03-23 | 2014-10-02 | Georges Balma | Method for obtaining a triploid interspecific hybrid of meagre and red drum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101699998B (en) * | 2009-11-03 | 2011-10-12 | 中国水产科学研究院黄海水产研究所 | Selection and culture method of excellent breed large-tooth flounder featuring fast growth and high survival rate |
| CN101911918A (en) * | 2010-03-18 | 2010-12-15 | 中国水产科学研究院黄海水产研究所 | Selective breeding method for disease-resistant superior strains of paralichthys olivaceus |
| CN101810148A (en) * | 2010-04-28 | 2010-08-25 | 中国水产科学研究院黄海水产研究所 | Cross breeding method of fine variety of paralichthysolivaceus |
| US20140298495A1 (en) * | 2011-03-23 | 2014-10-02 | Georges Balma | Method for obtaining a triploid interspecific hybrid of meagre and red drum |
| CN103005164A (en) * | 2012-12-29 | 2013-04-03 | 青岛农业大学 | Compound nucleotide immunoenhancer for paralichthys olivaceus |
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