CN107209190B - 用于富集循环肿瘤dna的方法 - Google Patents

用于富集循环肿瘤dna的方法 Download PDF

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CN107209190B
CN107209190B CN201580058989.4A CN201580058989A CN107209190B CN 107209190 B CN107209190 B CN 107209190B CN 201580058989 A CN201580058989 A CN 201580058989A CN 107209190 B CN107209190 B CN 107209190B
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nucleosomes
dna
histone
cancer
circulating
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CN107209190A (zh
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J.V.米卡莱夫
M.赫佐格
M.E.埃克莱斯顿
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Belgian Volition SPRL
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Priority claimed from GBGB1419225.6A external-priority patent/GB201419225D0/en
Priority claimed from GB201419299A external-priority patent/GB201419299D0/en
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Priority to CN202010018617.1A priority Critical patent/CN111505305B/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G01N27/622—Ion mobility spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/70—Mechanisms involved in disease identification
    • G01N2800/7023—(Hyper)proliferation
    • G01N2800/7028—Cancer

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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN201580058989.4A 2014-10-29 2015-10-29 用于富集循环肿瘤dna的方法 Active CN107209190B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010018617.1A CN111505305B (zh) 2014-10-29 2015-10-29 用于富集循环肿瘤dna的方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1419225.6A GB201419225D0 (en) 2014-10-29 2014-10-29 Method for the enrichment of circulating tumor DNA
GB1419225.6 2014-10-29
GB201419299A GB201419299D0 (en) 2014-10-30 2014-10-30 Method for the enrichment of circulating tumour DNA
GB1419299.1 2014-10-30
PCT/GB2015/053238 WO2016067029A1 (en) 2014-10-29 2015-10-29 Method for the enrichment of circulating tumor dna

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CN107209190A CN107209190A (zh) 2017-09-26
CN107209190B true CN107209190B (zh) 2021-07-16

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CN202010018617.1A Active CN111505305B (zh) 2014-10-29 2015-10-29 用于富集循环肿瘤dna的方法

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US (2) US20180024141A1 (https=)
EP (2) EP3809140A1 (https=)
JP (1) JP6721599B2 (https=)
CN (2) CN107209190B (https=)
AU (1) AU2015340378B2 (https=)
CA (1) CA2965752C (https=)
DK (1) DK3213084T4 (https=)
ES (1) ES2839973T3 (https=)
IL (1) IL251896A0 (https=)
PL (1) PL3213084T3 (https=)
SG (2) SG11201702978VA (https=)
WO (1) WO2016067029A1 (https=)

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HK1206055A1 (en) 2012-03-02 2015-12-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
HK1216655A1 (zh) 2013-03-13 2016-11-25 Sequenom, Inc. 用於dna甲基化分析的引物
US11365447B2 (en) 2014-03-13 2022-06-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN106544341A (zh) * 2017-01-17 2017-03-29 上海亿康医学检验所有限公司 高效检测样本中的ctDNA的方法
ES2913665T3 (es) * 2017-09-18 2022-06-03 Santersus Ag Método y dispositivo para la purificación de sangre a partir de ADN celular libre en circulación
CN109932224A (zh) * 2017-12-18 2019-06-25 中国科学院大连化学物理研究所 一种富集提取循环肿瘤dna的方法
CN112119166B (zh) * 2018-03-13 2025-08-22 耶路撒冷希伯来大学伊萨姆研发有限公司 无细胞dna染色质免疫沉淀的诊断应用
WO2019222657A1 (en) * 2018-05-18 2019-11-21 The Johns Hopkins University Cell-free dna for assessing and/or treating cancer
GB201818965D0 (en) 2018-11-21 2019-01-09 Belgian Volition Sprl Method for the detection of colorectal cancer
GB201818963D0 (en) 2018-11-21 2019-01-09 Belgian Volition Sprl Methof for the detection of prostate cancer
CN109841265B (zh) * 2019-02-22 2021-09-21 清华大学 使用片段化模式确定血浆游离核酸分子组织来源的方法和系统及应用
GB201906201D0 (en) 2019-05-02 2019-06-19 Belgian Voltion Sprl Method for the detection of protate cancer
GB201906199D0 (en) * 2019-05-02 2019-06-19 Belgian Volition Sprl Method for the detection of cancer
JP2022545821A (ja) * 2019-08-27 2022-10-31 ベルジアン ボリション エスアールエル 循環ヌクレオソームを単離する方法
EP4041914A1 (en) * 2019-10-04 2022-08-17 Santersus AG Method for isolating and analyzing cell free dna
JP7349104B2 (ja) * 2019-11-13 2023-09-22 株式会社島津製作所 ベロ毒素の検出方法
PL4070111T3 (pl) * 2019-12-02 2025-02-03 Belgian Volition Srl Zastosowanie wolnych pozakomórkowych nukleosomsów jako biomarkerów
CN116018646A (zh) * 2020-05-22 2023-04-25 阿克图尔公司 用于表征无细胞核酸片段的方法
GB202117799D0 (en) 2021-12-09 2022-01-26 Belgian Volition Srl Method for the detection of blood cancer
EP4453241A1 (en) * 2021-12-21 2024-10-30 Guardant Health, Inc. Methods and systems for combinatorial chromatin-ip sequencing
WO2023126672A1 (en) 2021-12-27 2023-07-06 Santersus Ag Method and device for removal of circulating cell free dna
WO2023170298A1 (en) 2022-03-11 2023-09-14 Belgian Volition Srl Differential diagnosis method
WO2024042210A1 (en) * 2022-08-25 2024-02-29 Belgian Volition Srl Method for measuring cell free chromatin
EP4646208A1 (en) 2023-01-06 2025-11-12 Ideaya Biosciences, Inc. Treatment of er+ breast cancer comprising homologous recombination deficiency using parg inhibitor
WO2024223947A1 (en) 2023-04-28 2024-10-31 Belgian Volition Srl Use of cell free nucleosomes as biomarkers of carcinomas
GB202307768D0 (en) 2023-05-24 2023-07-05 Belgian Volition Srl Use of cell free nucleosomes as biomarkers
GB202310137D0 (en) 2023-07-03 2023-08-16 Belgian Volition Srl Method for the detection of cancer
WO2025012440A1 (en) 2023-07-13 2025-01-16 Belgian Volition Srl Method for detection of the potential of a body fluid sample to produce extracellular traps
WO2025061853A1 (en) 2023-09-19 2025-03-27 Belgian Volition Srl Lateral flow test device
WO2025073909A1 (en) 2023-10-04 2025-04-10 Belgian Volition Srl Pcr method for detecting ctcf occupied cfdna fragments
WO2025093747A1 (en) 2023-11-02 2025-05-08 Belgian Volition Srl Novel rapid high-throughput method of netosis induction and inhibition with physiologically relevant molecules

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GB0319376D0 (en) † 2003-08-18 2003-09-17 Chroma Therapeutics Ltd Histone modification detection
US20060115482A1 (en) * 2004-04-27 2006-06-01 The Regents Of The University Of California Modifications of histone proteins as indicators of cell proliferation and differentiation
US20070243549A1 (en) * 2006-04-12 2007-10-18 Biocept, Inc. Enrichment of circulating fetal dna
EP2062912A1 (en) * 2007-11-26 2009-05-27 Koninklijke Philips Electronics N.V. Selective enrichment of post-translationally modified proteins and/or peptides
US20100240054A1 (en) * 2008-09-22 2010-09-23 Biocept, Inc. Identification and isolation of fetal cells and nucleic acid
BRPI1013546A2 (pt) * 2009-04-14 2019-04-09 The Regente Of The University Of California usos de um inibidor da timidilato sintase e de uma terapia anticâncer
US20140213475A1 (en) * 2011-07-14 2014-07-31 University Of Massachusetts Methods of diagnosing cancer using epigenetic biomarkers
GB201115098D0 (en) † 2011-09-01 2011-10-19 Belgian Volition Sa Method for detecting nucleosomes containing histone variants
GB201115095D0 (en) * 2011-09-01 2011-10-19 Singapore Volition Pte Ltd Method for detecting nucleosomes containing nucleotides
WO2013075237A1 (en) * 2011-11-21 2013-05-30 The Royal Institution For The Advancement Of Learning / Mcgill University Mutations of histone proteins associated with proliferative disorders
CN104067125B (zh) 2011-12-07 2017-12-26 比利时意志有限责任公司 用于检测核小体加合物的方法
HK1206055A1 (en) * 2012-03-02 2015-12-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9388213B2 (en) * 2012-09-10 2016-07-12 The Rockefeller University Polycomb repressive complex 2 (PRC2) inhibitors and uses thereof

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JP2017534300A (ja) 2017-11-24
SG10201903822XA (en) 2019-05-30
IL251896A0 (en) 2017-06-29
DK3213084T3 (da) 2021-01-18
EP3213084A1 (en) 2017-09-06
EP3809140A1 (en) 2021-04-21
US20180024141A1 (en) 2018-01-25
DK3213084T4 (da) 2024-03-18
SG11201702978VA (en) 2017-05-30
ES2839973T3 (es) 2021-07-06
CN111505305A (zh) 2020-08-07
AU2015340378B2 (en) 2021-08-12
CN111505305B (zh) 2023-10-17
CA2965752A1 (en) 2016-05-06
EP3213084B2 (en) 2023-12-13
CA2965752C (en) 2024-04-02
US20220334128A1 (en) 2022-10-20
PL3213084T3 (pl) 2021-07-26
JP6721599B2 (ja) 2020-07-15
NZ731116A (en) 2024-03-22
AU2015340378A1 (en) 2017-05-11
WO2016067029A1 (en) 2016-05-06
EP3213084B1 (en) 2020-11-25
HK1243764A1 (en) 2018-07-20
CN107209190A (zh) 2017-09-26

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