CN107208150A - 泡状引物 - Google Patents

泡状引物 Download PDF

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Publication number
CN107208150A
CN107208150A CN201580074738.5A CN201580074738A CN107208150A CN 107208150 A CN107208150 A CN 107208150A CN 201580074738 A CN201580074738 A CN 201580074738A CN 107208150 A CN107208150 A CN 107208150A
Authority
CN
China
Prior art keywords
primer
sequence
nucleotides
template
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580074738.5A
Other languages
English (en)
Chinese (zh)
Inventor
布莱恩·詹姆斯·麦考恩
卡塔尔·约瑟夫·麦克尔冈
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DNAE Group Holdings Ltd
Original Assignee
DNAE Group Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DNAE Group Holdings Ltd filed Critical DNAE Group Holdings Ltd
Publication of CN107208150A publication Critical patent/CN107208150A/zh
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580074738.5A 2014-12-22 2015-12-22 泡状引物 Pending CN107208150A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201422982 2014-12-22
GB1422982.7 2014-12-22
PCT/GB2015/054125 WO2016102956A1 (en) 2014-12-22 2015-12-22 Bubble primers

Publications (1)

Publication Number Publication Date
CN107208150A true CN107208150A (zh) 2017-09-26

Family

ID=55025267

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580074738.5A Pending CN107208150A (zh) 2014-12-22 2015-12-22 泡状引物

Country Status (5)

Country Link
US (1) US20170349926A1 (https=)
EP (1) EP3237635B1 (https=)
JP (1) JP2018500936A (https=)
CN (1) CN107208150A (https=)
WO (1) WO2016102956A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111699269A (zh) * 2018-01-12 2020-09-22 纳特拉公司 新引物及其用途

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018009677A1 (en) * 2016-07-07 2018-01-11 Complete Genomics, Inc. Fast target enrichment by multiplexed relay pcr with modified bubble primers
CN107365769B (zh) * 2017-07-25 2021-05-04 深圳华大智造科技股份有限公司 一种鼓泡状引物及其组成的试剂盒和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101189336A (zh) * 2005-03-05 2008-05-28 视基因公司 使用双特异性寡核苷酸的方法及所述双特异性寡核苷酸
CN101484898A (zh) * 2006-07-04 2009-07-15 株式会社岛津制作所 核酸扩增用引物设计装置、引物设计程序、以及引物设计服务器装置
CN102952798A (zh) * 2012-11-07 2013-03-06 龙岩九健生物芯片技术研究所 一种pcr引物设计方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
EP1896617B1 (en) * 2005-05-31 2013-01-02 Life Technologies Corporation Multiplex amplification of short nucleic acids
WO2011001496A1 (ja) * 2009-06-29 2011-01-06 株式会社 東芝 検体解析方法およびそれに用いるアッセイキット
WO2012103154A1 (en) * 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
CN103060924B (zh) * 2011-10-18 2016-04-20 深圳华大基因科技有限公司 微量核酸样本的文库制备方法及其应用
US20130184165A1 (en) * 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
HK1211058A1 (en) * 2012-07-24 2016-05-13 纳特拉公司 Highly multiplex pcr methods and compositions
JP2014180278A (ja) * 2013-03-21 2014-09-29 Toshiba Corp 核酸の解析方法、そこにおいて使用されるアッセイキット
CN111118125B (zh) * 2013-11-26 2023-06-30 杭州联川基因诊断技术有限公司 一种纯化pcr产物的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101189336A (zh) * 2005-03-05 2008-05-28 视基因公司 使用双特异性寡核苷酸的方法及所述双特异性寡核苷酸
CN101484898A (zh) * 2006-07-04 2009-07-15 株式会社岛津制作所 核酸扩增用引物设计装置、引物设计程序、以及引物设计服务器装置
CN102952798A (zh) * 2012-11-07 2013-03-06 龙岩九健生物芯片技术研究所 一种pcr引物设计方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CAIFU CHEN ET AL: "Real-time quantification of microRNAs by stem loop RT-PCR", 《NUCLEIC ACIDS RESEARCH》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111699269A (zh) * 2018-01-12 2020-09-22 纳特拉公司 新引物及其用途

Also Published As

Publication number Publication date
EP3237635B1 (en) 2019-10-23
JP2018500936A (ja) 2018-01-18
WO2016102956A1 (en) 2016-06-30
US20170349926A1 (en) 2017-12-07
EP3237635A1 (en) 2017-11-01

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